CN109846921B - A method for preparing Chinese medicinal extract with antiviral effect by high-speed countercurrent chromatography - Google Patents
A method for preparing Chinese medicinal extract with antiviral effect by high-speed countercurrent chromatography Download PDFInfo
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Abstract
The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a method for preparing a traditional Chinese medicine extract with an antiviral effect by using a high-speed countercurrent chromatography technology. The method comprises the following steps: (1) extracting the traditional Chinese medicine material of the zornia gibbosa with alcohol, concentrating the extracting solution and removing the solvent to obtain zornia gibbosa alcohol extract; (2) adding water to the alcohol extract of the zornia gibbosa for suspension, sequentially extracting with chloroform and ethyl acetate for 1-3 times respectively, taking an ethyl acetate extraction part, and concentrating to remove an ethyl acetate solvent to obtain an ethyl acetate extract; (3) separating the ethyl acetate extract by high-speed counter-current chromatography to obtain the Chinese medicinal extract. The herba Setariae extract has effects of resisting respiratory syncytial virus and influenza virus.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, in particular to a method for preparing a traditional Chinese medicine extract with an antiviral effect by utilizing a high-speed counter-current chromatography technology.
Background
The zornia gibbosa, named as traditional Chinese medicine, is the whole herb of zornia gibbosa of leguminosae. Collected in summer, the main functions are as follows: clearing away heat and toxic material, removing blood stasis, and treating common cold, hyperpyrexia, convulsion, diarrhea, jaundice, dysentery, infantile malnutrition, laryngalgia, conjunctival congestion, furuncle, toxic swelling, and mastitis.
At present, the existing literature has few research reports on the active ingredients with antiviral effect in the zornia gibbosa. The zornia gibbosa contains numerous chemical components, and research personnel need to further research and develop the effective parts with antiviral effects; in addition, since the types of viruses are numerous, whether the effective part in the zornia gibbosa has an antiviral effect on all viruses or on part of the viruses still needs to be researched and conquered through a large number of experiments by developers. Therefore, the traditional Chinese medicine of the zornia gibbosa is used as a raw material, and the development of a new antiviral drug has important significance for treating viral infection diseases.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for preparing a traditional Chinese medicine extract with antiviral effect by using a high-speed countercurrent chromatography technology in order to overcome the defects of the prior art in developing an antiviral effective part in zornia gibbosa. The herba Setariae extract has effects of resisting respiratory syncytial virus and influenza virus.
The technical problem to be solved by the invention is realized by the following technical scheme:
a method for preparing Chinese medicinal extract with antiviral effect by high-speed countercurrent chromatography comprises the following steps:
(1) extracting the traditional Chinese medicine material of the zornia gibbosa with alcohol, concentrating the extracting solution and removing the solvent to obtain zornia gibbosa alcohol extract;
(2) adding water to the alcohol extract of the zornia gibbosa for suspension, sequentially extracting with chloroform and ethyl acetate for 1-3 times respectively, taking an ethyl acetate extraction part, and concentrating to remove an ethyl acetate solvent to obtain an ethyl acetate extract;
(3) separating the ethyl acetate extract by high-speed counter-current chromatography to obtain the Chinese medicinal extract;
the specific method of the high-speed countercurrent chromatography in the step (3) comprises the following steps: the adopted solvent consists of normal hexane, ethyl acetate, methanol and water; wherein the volume ratio of the n-hexane to the ethyl acetate to the methanol to the water is 1-2: 3-4: 1-2; the high-speed counter-current chromatography host rotates clockwise at the rotating speed of 700-1000 rpm; the stationary phase retention value is: 60-80%, and the flow rate of the mobile phase is 1-2 ml/min; the separation temperature is 15-30 ℃; the ultraviolet detection wavelength is 280-330 nm; and (3) sampling and collecting the eluted part for 15-21 min to obtain the traditional Chinese medicine extract.
Most preferably, the volume ratio of the n-hexane to the ethyl acetate to the methanol to the water is 1:1.5:3.5: 1.5.
Most preferably, the high-speed counter-current chromatography host rotates clockwise at 855 rpm; the stationary phase retention value is: 72 percent, and the flow rate of the mobile phase is 1.5 ml/min; the separation temperature is 25 ℃; the ultraviolet detection wavelength is 305 nm.
Preferably, the extraction in step (1) is a heating reflux extraction.
Preferably, the alcohol extraction in the step (1) is ethanol water with a volume fraction of 50-80%.
Most preferably, the alcohol extraction in step (1) is 70% by volume of an aqueous ethanol solution.
Most preferably, the heating reflux extraction times are 1-3 times, and the extraction time of each time is 1-2 hours; wherein the dosage ratio of the traditional Chinese medicinal material of the zornia gibbosa to the ethanol water solution is 1 g: 12-18 mL.
Most preferably, the heating reflux extraction times are 1 time, and the extraction time of each time is 1.5 h; wherein the dosage ratio of the traditional Chinese medicinal material of the zornia gibbosa to the ethanol water solution is 1 g: 15 mL.
Preferably, the ratio of the alcohol extract of the zornia gibbosa to the water in the step (2) is 1 g: 15-30 mL; the volume usage ratio of chloroform to ethyl acetate to water in each extraction process is 1: 0.5-1.5.
Most preferably, the ratio of the alcohol extract of the zornia gibbosa to the water in the step (2) is 1 g: 20 mL; the volume usage of chloroform and ethyl acetate to the volume usage of water in each extraction process is 1:1.
Preferably, the virus is respiratory syncytial virus or influenza virus.
The invention also provides a traditional Chinese medicine extract prepared by the method.
The invention also provides application of the traditional Chinese medicine extract in preparation of antiviral drugs.
Preferably, the virus is respiratory syncytial virus or influenza virus.
Has the advantages that: the invention takes the traditional Chinese medicine of the zornia gibbosa as the raw material, and provides a brand new preparation method of the antiviral traditional Chinese medicine extract; the method adopts a traditional Chinese medicine modernization technology, and prepares a traditional Chinese medicine extract with respiratory syncytial virus or influenza virus resistance from a traditional Chinese medicine, namely zornia gibbosa, by utilizing a high-speed countercurrent chromatography technology for the first time; the experiments of the embodiments prove that the traditional Chinese medicine extract has the effect of resisting the respiratory syncytial virus or the influenza virus, wherein the effect of resisting the respiratory syncytial virus is most obvious.
Detailed Description
The present invention is further explained below with reference to specific examples, which are not intended to limit the present invention in any way.
Example 1 preparation of herbal extracts
Taking a traditional Chinese medicine, namely, zornia gibbosa, and carrying out heating reflux extraction for 1 time by using ethanol with the volume fraction of 70%, wherein the extraction time is 1.5 h; concentrating the extract, removing the solvent to obtain an alcohol extract of the zornia gibbosa; wherein the dosage ratio of the traditional Chinese medicinal material of the zornia gibbosa to the ethanol water solution is 1 g: 15 mL;
(2) suspending the alcohol extract of the zornia gibbosa with water, sequentially extracting with chloroform and ethyl acetate for 2 times, respectively, combining ethyl acetate extraction parts obtained by 2-time extraction, and concentrating to remove ethyl acetate solvent to obtain an ethyl acetate extract; wherein the dosage ratio of the alcohol extract of the zornia gibbosa to the water is 1 g: 20 mL; the volume usage ratio of chloroform to ethyl acetate to water in each extraction process is 1: 1;
(3) separating the ethyl acetate extract by high-speed counter-current chromatography to obtain the Chinese medicinal extract;
the specific operation of the high-speed counter-current chromatography is as follows:
(3.1) mixing n-hexane, ethyl acetate, methanol and water according to a volume ratio of 1:1.5:3.5:1.5, fully shaking and uniformly mixing the mixture, standing the mixture for layering to obtain an upper phase solution and a lower phase solution, separating the upper phase solution from the lower phase solution, ultrasonically degassing the upper phase solution for 30min, taking the upper phase solution as a mobile phase, taking the lower phase solution as a stationary phase, starting a sample injection pump to pump the stationary phase into and fill a countercurrent chromatographic column at the maximum flow rate of equipment, starting a high-speed countercurrent chromatographic host, a detector and a chromatographic workstation, and pumping the mobile phase at a rotation speed of 855rpm and a flow rate of 1.5 ml/min;
(3.2) dissolving the ethyl acetate extract in the lower phase solution to obtain a sample solution, and then filling the sample solution into a sample injection test tube; wherein the dosage ratio of the ethyl acetate extract to the lower phase solution is 2mg:1 mL;
(3.3) placing the sample injection filter head into a test tube, pumping the sample solution into a countercurrent chromatography pipeline at the flow rate of 1.5ml/min, continuously pumping the mobile phase at the flow rate of 1.5ml/min after the sample solution is completely fed, and collecting the elution part for 15-21 min after sample injection to obtain the traditional Chinese medicine extract;
wherein the high-speed counter-current chromatography host rotates clockwise, the retention value of the stationary phase is 72%, and the detection wavelength is 305 nm; the separation temperature was 25 ℃.
Comparative example 1 preparation of Chinese medicine extract
Taking a traditional Chinese medicine, namely, zornia gibbosa, and carrying out heating reflux extraction for 1 time by using ethanol with the volume fraction of 70%, wherein the extraction time is 1.5 h; concentrating the extract, removing the solvent to obtain an alcohol extract of the zornia gibbosa; wherein the dosage ratio of the traditional Chinese medicinal material of the zornia gibbosa to the ethanol water solution is 1 g: 15 mL;
(2) suspending the alcohol extract of the zornia gibbosa with water, sequentially extracting with chloroform and ethyl acetate for 2 times, respectively, combining ethyl acetate extraction parts obtained by 2-time extraction, and concentrating to remove ethyl acetate solvent to obtain an ethyl acetate extract; wherein the dosage ratio of the alcohol extract of the zornia gibbosa to the water is 1 g: 20 mL; the volume usage ratio of chloroform to ethyl acetate to water in each extraction process is 1: 1;
(3) separating the ethyl acetate extract by high-speed counter-current chromatography to obtain the Chinese medicinal extract;
the specific operation of the high-speed counter-current chromatography is as follows:
(3.1) mixing n-hexane, ethyl acetate, methanol and water according to a volume ratio of 2:3.5:2.5:1.5, fully shaking and uniformly mixing the mixture, standing the mixture for layering to obtain an upper phase solution and a lower phase solution, separating the upper phase solution from the lower phase solution, ultrasonically degassing the upper phase solution for 30min, taking the upper phase solution as a mobile phase, taking the lower phase solution as a stationary phase, starting a sample injection pump to pump the stationary phase into and fill a countercurrent chromatographic column at the maximum flow rate of equipment, starting a high-speed countercurrent chromatographic host, a detector and a chromatographic workstation, and pumping the mobile phase at the rotation speed of 855rpm and the flow rate of 1.5 ml/min;
(3.2) dissolving the ethyl acetate extract in the lower phase solution to obtain a sample solution, and then filling the sample solution into a sample injection test tube; wherein the dosage ratio of the ethyl acetate extract to the lower phase solution is 2mg:1 mL;
(3.3) placing the sample injection filter head into a test tube, pumping the sample solution into a countercurrent chromatography pipeline at the flow rate of 1.5ml/min, continuously pumping the mobile phase at the flow rate of 1.5ml/min after the sample solution is completely fed, and collecting the elution part for 15-21 min after sample injection to obtain the traditional Chinese medicine extract;
wherein the high-speed counter-current chromatography host rotates clockwise, the retention value of the stationary phase is 72%, and the detection wavelength is 305 nm; the separation temperature was 25 ℃.
The difference between the comparative example 1 and the example 1 is that the ratio of each component in the solvent in the high-speed counter-current chromatography is different, and the other steps and condition parameters are the same. The proportion of each solvent in comparative example 1 is: the volume ratio of the n-hexane to the ethyl acetate to the methanol to the water is 2:3.5:2.5: 1.5. In example 1, the volume ratio of n-hexane, ethyl acetate, methanol and water was 1:1.5:3.5: 1.5.
Comparative example 2 preparation of Chinese medicine extract
Taking a traditional Chinese medicine, namely, zornia gibbosa, and carrying out heating reflux extraction for 1 time by using ethanol with the volume fraction of 70%, wherein the extraction time is 1.5 h; concentrating the extract, removing the solvent to obtain an alcohol extract of the zornia gibbosa; wherein the dosage ratio of the traditional Chinese medicinal material of the zornia gibbosa to the ethanol water solution is 1 g: 15 mL;
(2) suspending the alcohol extract of the zornia gibbosa with water, sequentially extracting with chloroform and ethyl acetate for 2 times, respectively, combining ethyl acetate extraction parts obtained by 2-time extraction, and concentrating to remove ethyl acetate solvent to obtain an ethyl acetate extract; wherein the dosage ratio of the alcohol extract of the zornia gibbosa to the water is 1 g: 20 mL; the volume usage ratio of chloroform to ethyl acetate to water in each extraction process is 1: 1;
(3) separating the ethyl acetate extract by high-speed counter-current chromatography to obtain the Chinese medicinal extract;
the specific operation of the high-speed counter-current chromatography is as follows:
(3.1) mixing n-hexane, ethyl acetate, methanol and water according to a volume ratio of 1:1.5:3.5:1.5, fully shaking and uniformly mixing the mixture, standing the mixture for layering to obtain an upper phase solution and a lower phase solution, separating the upper phase solution from the lower phase solution, ultrasonically degassing the upper phase solution for 30min, taking the upper phase solution as a mobile phase, taking the lower phase solution as a stationary phase, starting a sample injection pump to pump the stationary phase into and fill a countercurrent chromatographic column at the maximum flow rate of equipment, starting a high-speed countercurrent chromatographic host, a detector and a chromatographic workstation, and pumping the mobile phase at a rotation speed of 855rpm and a flow rate of 1.5 ml/min;
(3.2) dissolving the ethyl acetate extract in the lower phase solution to obtain a sample solution, and then filling the sample solution into a sample injection test tube; wherein the dosage ratio of the ethyl acetate extract to the lower phase solution is 2mg:1 mL;
(3.3) placing the sample injection filter head into a test tube, pumping the sample solution into a countercurrent chromatography pipeline at the flow rate of 1.5ml/min, continuously pumping the mobile phase at the flow rate of 1.5ml/min after the sample solution is completely fed, and collecting the elution part for 25-33 min after sample injection to obtain the traditional Chinese medicine extract;
wherein the high-speed counter-current chromatography host rotates clockwise, the retention value of the stationary phase is 72%, and the detection wavelength is 305 nm; the separation temperature was 25 ℃.
Comparative example 2 is different from example 1 in that elution sites collected after sample injection in high-speed countercurrent chromatography are different, and the rest of the steps and condition parameters are the same. Collecting the elution part of 25-33 min after the middle sample injection of the comparative example 2; and the elution part of 15-21 min is collected after middle sample injection in the embodiment 1.
Experimental example: antiviral activity test of Chinese medicinal extract
Collecting 96-well cell culture plate full of single MDCK cell layer, discarding supernatant, washing with PBS twice, and adding 100TCID50After adsorbing 100. mu.L of virus solution per well for 1 hour, 100. mu.L of the diluted MEM solution was added to each well, and a cell control group, a virus control group, and a blank control group were set. Placing at 37 deg.C and saturated humidity with 5% CO2And (5) incubation in an incubator. After 48h, the old solution in the 96-well plate is discarded, and 100. mu.L of CCK8 original solution is respectively added into the tested hole after ten-fold dilution. Incubating in incubator for 1h, and culturingMeasuring absorbance at 450nm by enzyme-linked immunosorbent assay (ELISA) instrument, and calculating IC50. The test results are shown in Table 1, IC in the test results50Smaller values indicate better anti-herpes virus effect.
The virus solution is prepared into virus solution containing Respiratory Syncytial Virus (RSV) or influenza virus (H1N1) according to detection requirements. The liquid medicine respectively contains the traditional Chinese medicine extracts of example 1, comparative example 1 and comparative example 2 and is used for testing the antiviral effect. IC (integrated circuit)50The calculation method is as follows: the cell survival rate is (drug adding well OD value-virus control group OD value)/(cell control group OD value-virus control group OD value) × 100%; the concentration corresponding to 50% cell viability was determined as IC by plotting the drug concentration on the X-axis and the cell viability on the Y-axis50。
TABLE 1 antiviral activity test results of Chinese medicinal extracts
IC from Table 150The data show that the traditional Chinese medicine extract of the zornia gibbosa prepared under the specific conditions of the high-speed countercurrent chromatography has the effect of resisting respiratory syncytial virus and influenza virus; the effect of resisting the respiratory syncytial virus is the most remarkable and is far better than that of the influenza virus.
Furthermore, IC by comparative example 1 and example 150The data show that the anti-respiratory syncytial virus and anti-influenza virus effect of the traditional Chinese medicine extract of the zornia gibbosa prepared in the comparative example 1 is greatly reduced, which indicates that the proportion of each component in the solvent in the high-speed countercurrent chromatography is a key factor influencing the enrichment of the anti-virus effective components in the traditional Chinese medicine extract of the zornia gibbosa; and under the solvent ratio of the embodiment 1, a large amount of anti-respiratory syncytial virus and influenza virus components, especially anti-respiratory syncytial virus components can be enriched. IC by comparison of example 2 and example 150As can be seen from the data, the extract of the traditional Chinese medicine of the zornia gibbosa prepared in the comparative example 2 does not have the antiviral effect, which indicates that the elution part is opposite to the effective part in the high-speed counter-current chromatographyThe method plays a decisive role, and a large amount of components resisting respiratory syncytial virus and influenza virus, particularly components resisting respiratory syncytial virus, are enriched at an elution part which is collected for 15-21 min after sample injection in the embodiment 1; the antiviral component cannot be enriched at other elution sites.
Claims (7)
1. A method for preparing a traditional Chinese medicine extract with antiviral effect by using a high-speed counter-current chromatography technology is characterized by comprising the following steps:
(1) extracting the traditional Chinese medicine material of the zornia gibbosa with alcohol, concentrating the extracting solution and removing the solvent to obtain zornia gibbosa alcohol extract;
(2) adding water to the alcohol extract of the zornia gibbosa for suspension, sequentially extracting with chloroform and ethyl acetate for 1-3 times respectively, taking an ethyl acetate extraction part, and concentrating to remove an ethyl acetate solvent to obtain an ethyl acetate extract;
(3) separating the ethyl acetate extract by high-speed counter-current chromatography to obtain the Chinese medicinal extract;
the specific method of the high-speed countercurrent chromatography in the step (3) comprises the following steps: the adopted solvent consists of normal hexane, ethyl acetate, methanol and water; wherein the volume ratio of the n-hexane to the ethyl acetate to the methanol to the water is 1:1.5:3.5: 1.5; the high-speed counter-current chromatography host rotates clockwise at the rotating speed of 700-1000 rpm; the stationary phase retention value is: 60-80%, and the flow rate of the mobile phase is 1-2 ml/min; the separation temperature is 15-30 ℃; the ultraviolet detection wavelength is 280-330 nm; sampling and collecting the eluted part for 15-21 min to obtain the traditional Chinese medicine extract;
the extraction in the step (1) is heating reflux extraction; the alcohol extraction refers to the use of ethanol water solution with the volume fraction of 70%.
2. The method for preparing herbal extracts with antiviral effect by high speed countercurrent chromatography as claimed in claim 1, wherein the high speed countercurrent chromatography host is rotated clockwise at 855 rpm; the stationary phase retention value is: 72 percent, and the flow rate of the mobile phase is 1.5 ml/min; the separation temperature is 25 ℃; the ultraviolet detection wavelength is 305 nm.
3. The method for preparing herbal extract with antiviral effect by high speed countercurrent chromatography as claimed in claim 1, wherein the number of times of heating reflux extraction is 1, and the extraction time per time is 1.5 h; wherein the dosage ratio of the traditional Chinese medicinal material of the zornia gibbosa to the ethanol water solution is 1 g: 15 mL.
4. The method for preparing herbal extracts having antiviral effects according to claim 1, wherein the ratio of the alcohol extract of bucarb to water in step (2) is 1 g: 20 mL; the volume usage of chloroform and ethyl acetate to the volume usage of water in each extraction process is 1:1.
5. The method for preparing herbal extract with antiviral effect according to claim 1, wherein the virus is respiratory syncytial virus or influenza virus.
6. A herbal extract prepared according to any one of claims 1 to 5.
7. The use of the herbal extract of claim 6 in the preparation of an antiviral medicament.
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