CN109827989B - Domestic blood fluorine concentration detect reagent box - Google Patents
Domestic blood fluorine concentration detect reagent box Download PDFInfo
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- CN109827989B CN109827989B CN201910112291.6A CN201910112291A CN109827989B CN 109827989 B CN109827989 B CN 109827989B CN 201910112291 A CN201910112291 A CN 201910112291A CN 109827989 B CN109827989 B CN 109827989B
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- blood
- fluorine
- iron complex
- fluorine concentration
- concentration
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- 239000011737 fluorine Substances 0.000 title claims abstract description 51
- 229910052731 fluorine Inorganic materials 0.000 title claims abstract description 51
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 title claims abstract description 49
- 239000008280 blood Substances 0.000 title claims abstract description 49
- 210000004369 blood Anatomy 0.000 title claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 title description 3
- 150000004698 iron complex Chemical class 0.000 claims abstract description 30
- 238000001514 detection method Methods 0.000 claims abstract description 29
- 239000000243 solution Substances 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 13
- 239000011259 mixed solution Substances 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 claims description 15
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- 229910052742 iron Inorganic materials 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- -1 fluorine ions Chemical class 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- DCYOBGZUOMKFPA-UHFFFAOYSA-N iron(2+);iron(3+);octadecacyanide Chemical compound [Fe+2].[Fe+2].[Fe+2].[Fe+3].[Fe+3].[Fe+3].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] DCYOBGZUOMKFPA-UHFFFAOYSA-N 0.000 claims description 2
- 229960003351 prussian blue Drugs 0.000 claims description 2
- 239000013225 prussian blue Substances 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 208000004042 dental fluorosis Diseases 0.000 description 8
- 206010016818 Fluorosis Diseases 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229940074391 gallic acid Drugs 0.000 description 2
- 235000004515 gallic acid Nutrition 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 206010074268 Reproductive toxicity Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001201 calcium accumulation Effects 0.000 description 1
- 238000013170 computed tomography imaging Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000002595 magnetic resonance imaging Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000007696 reproductive toxicity Effects 0.000 description 1
- 231100000372 reproductive toxicity Toxicity 0.000 description 1
- 239000010421 standard material Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a household blood fluorine concentration detection kit. Consists of a temperature measuring device and an iron complex solution. The method for quantitatively measuring the concentration of the fluorine in the blood by using the kit comprises the following steps: 1) drawing a standard curve: gathering sunlight by using a condensing lens, and measuring the temperature change of a series of iron complex solutions containing different fluorine concentrations under the irradiation of the sunlight within the same time; performing linear fitting to obtain a standard curve; 2) and (3) detection of blood fluorine concentration: taking a blood sample to be detected, carrying out mixing reaction with the iron complex solution to obtain a mixed solution, measuring the temperature change of the mixed solution in the same time as that in the step 1) under the same sunlight irradiation, and comparing with the standard curve in the step 1) to obtain the concentration of the blood fluorine. The invention utilizes a household thermometer to measure the temperature signal change of the iron complex solution under the irradiation of sunlight, and realizes the rapid and sensitive quantitative detection of the concentration of the blood fluorine.
Description
Technical Field
The invention belongs to the technical field of analysis and detection, and particularly relates to a household blood fluorine concentration detection kit.
Background
Fluorine is an important constant element in the human body and exists mainly in the form of fluoride ions. However, excessive fluoride ion can lead to physiological phenomena such as reproductive toxicity, skeletal embrittlement, abnormal calcium accumulation and dental fluorosis, which are commonly referred to as manifestations of fluorosis. Fluorine ions can be taken in by human bodies in various ways such as inhalation, ingestion, skin contact and the like, so that the disease of fluorosis has higher morbidity in a plurality of countries including China. The traditional fluorine poisoning diagnosis means mainly rely on molecular imaging technology, such as magnetic resonance imaging and computed tomography imaging. However, the molecular imaging technology has the problems of long testing time, high price and the like, and is not suitable for daily fluorosis diagnosis.
Recent studies have shown that blood fluorine concentration is one of the important markers in the diagnosis of fluorosis. Therefore, quantifying blood fluorine concentration can effectively diagnose fluorosis. However, conventional fluorescence or colorimetric kits are difficult to achieve accurate quantification of blood fluorine concentrations at home due to the need to use expensive specialized instruments.
Disclosure of Invention
The invention aims to provide a household blood fluorine concentration detection kit. The invention utilizes a household thermometer to measure the temperature signal change of the iron complex solution under the irradiation of sunlight, and realizes the rapid and sensitive quantitative detection of the concentration of the blood fluorine.
The invention provides a household blood fluorine concentration detection kit, which consists of a temperature measuring device and an iron complex solution.
The solute of the iron complex solution is iron complex, and the solvent is water.
The iron complex can be an iron complex with strong absorption in the near infrared (750-1500nm) wavelength range, and includes but is not limited to prussian blue, gallic acid-iron (III) complex, catechin-iron (III) complex, 1,2, 3-pyrogallol-iron (III) complex and the like, and can be particularly gallic acid-iron (III) complex.
The mass fraction of the iron complex solution can be 5-55%, and specifically can be 20%.
When the iron complex solution is gallic acid-iron (III) complex solution, the preparation method comprises the following steps: mixing aqueous solution of gallic acid with FeCl3Mixing the aqueous solutions, reacting under stirring, distilling under reduced pressure, collecting solid, and dissolving the obtained solid in water to obtain gallic acid-iron (III) complex solution.
The temperature measuring device can be a thermometer, in particular a household thermometer, and the measuring range can be 0-100 ℃.
The domestic blood fluorine concentration detection kit can further comprise a condenser lens.
The application of the domestic blood fluorine concentration detection kit in the preparation of the detection kit for quantitatively determining the blood fluorine concentration also belongs to the protection scope of the invention.
The invention also provides a method for quantitatively determining the blood fluorine concentration by using the household blood fluorine concentration detection kit.
The method for quantitatively measuring the blood fluorine concentration by using the household blood fluorine concentration detection kit provided by the invention comprises the following steps:
1) drawing a standard curve: gathering sunlight by using a condensing lens, and measuring the temperature change of a series of iron complex solutions containing different fluorine concentrations under the irradiation of the sunlight within the same time; taking the temperature change as a vertical coordinate, taking the fluoride ion concentration contained in the series of iron complex solutions containing different fluoride concentrations as a horizontal coordinate, and performing linear fitting to obtain a standard curve;
2) and (3) detection of blood fluorine concentration: taking a blood sample to be detected, carrying out mixing reaction with a blank iron complex solution to obtain a mixed solution, measuring the temperature change of the mixed solution under the same sunlight irradiation in the same time as the step 1), and finally comparing with the standard curve obtained in the step 1) to obtain the blood fluorine concentration of the blood sample to be detected.
In the method, the temperature change of a series of iron complex solutions with different fluorine concentrations in the step 1) and the temperature change of the mixed solution in the step 2) are measured by adopting a temperature monitoring method.
The temperature monitoring method adopts a thermometer as a temperature measuring instrument.
The same time in step 1) and 2) can be 0.5-60min, specifically 4 min.
In the step 2), the reaction temperature can be 0-40 ℃, the reaction time can be 0.5-60min, and specifically the reaction can be carried out at 25 ℃ for 10 min.
The fluoride ion concentration in the series of iron complex solutions containing different fluorine concentrations can be 0-100 mu mol/L, and specifically can be 0 mu mol/L, 20 mu mol/L, 35 mu mol/L and 50 mu mol/L.
In the step 2), the volume ratio of the blood sample to be detected to the iron complex solution can be 0.5-5: 1, specifically 500. mu.L: 1000. mu.L.
The invention realizes sensitive and accurate quantitative detection of the concentration of the blood fluorine by using the temperature signal change of the iron complex under the irradiation of sunlight. Specifically, a linear graph (regression coefficient R) of the temperature signal change and the fluorine ion concentration of a series of iron complex solutions with known fluorine ion concentrations under the irradiation of sunlight is measured2Not less than 0.99), obtaining a standard linear map; and measuring the temperature of the sample to be measured with unknown fluoride ion concentration, and comparing the temperature with the standard linear map to obtain the fluorine ion concentration measuring sample.
Compared with the prior art, the invention has the following beneficial effects: the kit can realize signal reading by using a household thermometer, can use sunlight as an excitation light source, does not need any expensive professional instrument, and is more suitable for daily blood fluorine concentration detection used at home.
Drawings
FIG. 1 is a standard curve obtained on sunny days using the kit according to example 1 of the present invention.
FIG. 2 is a standard curve obtained on cloudy days using the kit in example 1 of the present invention.
FIG. 3 is a graph showing the blood fluorine concentrations of normal mice and fluorosis mice measured on a sunny day using the kit of example 1 of the present invention.
FIG. 4 is a graph showing the blood fluorine concentrations of normal mice and fluorosis mice measured on a cloudy day using the kit in example 1 of the present invention.
Detailed Description
The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, materials and the like used in the following examples are commercially available unless otherwise specified.
The gallic acid-iron (III) complex used in the following example 1 was prepared as follows: mixing aqueous solution of gallic acid with a molar concentration of 6mmol/L and FeCl with a molar concentration of 1mmol/L3Mixing the water solutions in equal volume, stirring at 25 deg.C for 120min, distilling under reduced pressure, collecting solid, and dissolving in deionized water to obtain 20% gallic acid-iron (III) complex water solution.
The embodiment 1, the domestic blood fluorine concentration detection kit of the invention comprises the following components and a using method thereof:
composition of domestic blood fluorine concentration detection kit
Detection kit (50 parts):
secondly, the use method of the kit comprises the following steps:
blood is collected as a detection sample, and the fluorine concentration of the blood is detected according to the following method:
1) drawing a standard curve: the sunlight is collected by the collecting lens and irradiated by the sunlightNext, the temperature change of the standard solution in 4 minutes was measured, and the data was processed to obtain a standard curve of the fluoride ion concentration and the temperature change. As can be seen from fig. 1 and 2: the standard map has good linearity in the concentration range of 0-50 mu mol/L in sunny days and cloudy days, and the correlation coefficient R2Reaching 0.9994 and 0.9996 respectively.
2) And (3) detection of blood fluorine concentration: uniformly mixing 500 mu L of a detection sample with 1000 mu L of gallic acid-iron (III) complex aqueous solution with the mass fraction of 20%, standing at room temperature (25 ℃) for reaction for 10min, measuring the temperature change of the mixed solution within 4min under the irradiation of sunlight to obtain a temperature change value, and substituting the temperature change value into the standard curve obtained in the step 1) to obtain the accurate fluoride ion concentration of the detection sample. As can be seen from FIGS. 3 and 4, the relative error of the fluoride ion concentration of the sample to be detected measured by using the kit of the present invention is less than 0.05%, which is negligible, compared with the standard electrochemical method (magic Bettinelli, Determination of fluoride in environmental standard materials with a fluoride ion-selective electrode, analysis, 1983,108,404-407), indicating that the measurement result of the present invention is sensitive and accurate.
Claims (8)
1. A domestic blood fluorine concentration detection kit comprises a temperature measuring device and an iron complex solution;
the solute of the iron complex solution is iron complex, and the solvent is water;
the iron complex is at least one of prussian blue, a gallic acid-iron III complex, a catechin-iron III complex and a 1,2, 3-pyrogallol-iron III complex;
the temperature measuring device is a thermometer.
2. The kit for detecting blood fluorine concentration for home use according to claim 1, wherein: the mass fraction of the iron complex solution is 5-55%.
3. The kit for detecting blood fluorine concentration for home use according to claim 1 or 2, characterized in that: the domestic blood fluorine concentration detection kit further comprises a condenser lens.
4. Use of the home-use blood fluorine concentration detection kit according to any one of claims 1 to 3 for the preparation of a detection kit for the quantitative determination of blood fluorine concentration.
5. A method for quantitative determination of blood fluorine concentration using the home-use blood fluorine concentration detection kit according to any one of claims 1 to 3, comprising the steps of:
1) drawing a standard curve: gathering sunlight by using a condensing lens, and measuring the temperature change of a series of iron complex solutions containing different fluorine concentrations under the irradiation of the sunlight within the same time; taking the temperature change as a vertical coordinate, taking the fluoride ion concentration contained in the series of iron complex solutions containing different fluoride concentrations as a horizontal coordinate, and performing linear fitting to obtain a standard curve;
2) and (3) detection of blood fluorine concentration: taking a blood sample to be detected, carrying out mixing reaction with a blank iron complex solution to obtain a mixed solution, measuring the temperature change of the mixed solution under the same sunlight irradiation in the same time as the step 1), and comparing with the standard curve obtained in the step 1) to obtain the blood fluorine concentration of the blood sample to be detected.
6. The method of claim 5, wherein: in the step 1), the concentration of fluorine ions in the series of iron complex solutions with different fluorine concentrations is 0-100 mu mol/L.
7. The method according to claim 5 or 6, characterized in that: in the step 2), the reaction temperature is 0-40 ℃, and the reaction time is 0.5-60 min.
8. The method according to claim 5 or 6, characterized in that: in the step 2), the volume ratio of the blood sample to be detected to the iron complex solution is 0.5-5: 1.
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| CN201910112291.6A CN109827989B (en) | 2019-02-13 | 2019-02-13 | Domestic blood fluorine concentration detect reagent box |
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| CN201910112291.6A CN109827989B (en) | 2019-02-13 | 2019-02-13 | Domestic blood fluorine concentration detect reagent box |
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| CN109827989B true CN109827989B (en) | 2021-07-09 |
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| JP3781023B2 (en) * | 2003-08-21 | 2006-05-31 | 日立電線株式会社 | ELECTROLYTE MEMBRANE FOR FUEL CELL AND METHOD FOR PRODUCING THE SAME |
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| CN101391979A (en) * | 2008-10-24 | 2009-03-25 | 中南民族大学 | Unsymmetrical bis(imino)pyridines iron and cobalt complexes containing halogen, preparation method and use |
| CN102633840A (en) * | 2012-04-05 | 2012-08-15 | 吉林大学 | Clamp-type iron complex and preparation method and application thereof |
| CN105412059A (en) * | 2015-12-02 | 2016-03-23 | 江苏省中国科学院植物研究所 | New application of ethyl gallate to osteosarcoma treatment |
| CN106442430A (en) * | 2015-08-05 | 2017-02-22 | 首都师范大学 | Solution concentration detection method based on photothermal conversion nano-material temperature change |
-
2019
- 2019-02-13 CN CN201910112291.6A patent/CN109827989B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3781023B2 (en) * | 2003-08-21 | 2006-05-31 | 日立電線株式会社 | ELECTROLYTE MEMBRANE FOR FUEL CELL AND METHOD FOR PRODUCING THE SAME |
| CN101182299A (en) * | 2006-07-31 | 2008-05-21 | 赛德玛公司 | Mydrial derivates,preparation method of mydrial derivates and cosmetics or skin medicinal composition |
| CN101246148A (en) * | 2007-11-19 | 2008-08-20 | 中国海洋大学 | Method for detecting external activity of angiotonin enzyme inhibition peptide |
| CN101391979A (en) * | 2008-10-24 | 2009-03-25 | 中南民族大学 | Unsymmetrical bis(imino)pyridines iron and cobalt complexes containing halogen, preparation method and use |
| CN102633840A (en) * | 2012-04-05 | 2012-08-15 | 吉林大学 | Clamp-type iron complex and preparation method and application thereof |
| CN106442430A (en) * | 2015-08-05 | 2017-02-22 | 首都师范大学 | Solution concentration detection method based on photothermal conversion nano-material temperature change |
| CN105412059A (en) * | 2015-12-02 | 2016-03-23 | 江苏省中国科学院植物研究所 | New application of ethyl gallate to osteosarcoma treatment |
Non-Patent Citations (1)
| Title |
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