CN109825539A - A kind of method of biosynthesis Clary sage lactone - Google Patents
A kind of method of biosynthesis Clary sage lactone Download PDFInfo
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- CN109825539A CN109825539A CN201910275371.3A CN201910275371A CN109825539A CN 109825539 A CN109825539 A CN 109825539A CN 201910275371 A CN201910275371 A CN 201910275371A CN 109825539 A CN109825539 A CN 109825539A
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- clary sage
- sage lactone
- culture medium
- biosynthesis
- yeast culture
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Abstract
A kind of method of biosynthesis Clary sage lactone takes yeast culture medium and is separately added into the sclareol of yeast culture medium quality 3%~8% thereto and 4%~10% shallow white Cryptococcus plasm body fluid obtains mixture；Mixture is placed in glass container, then obtains Clary sage lactone within shake flask fermentation 10 days on 23-28 DEG C, the shaking table of 140~180r/min.The present invention utilizes microbe transformation method, using sclareol as substrate, conversion, which is carried out, using shallow white Cryptococcus prepares Clary sage lactone, the problems such as solving the unfriendly environment occurred in chemical method synthesis Clary sage lactone technical process, complex steps, low safety, provides new way for the synthesis of Clary sage lactone.
Description
Technical field
The invention belongs to field of food, and in particular to a kind of method of biosynthesis Clary sage lactone.
Background technique
Clary sage lactone is a kind of important food additives, belongs to ambergris-like perfume, is the weight for synthesizing ambrox
Want intermediate.Clary sage lactone is capable of increasing and improves the organoleptic attribute of food, is widely used for food industry.It can also be extensive
Applied to industries such as tobacco and cosmetics, meanwhile, Clary sage lactone is also the intermediate of some pharmaceutical synthesis, is applied to medical row
Industry can synthesize the drug with anticancer function.Therefore, the synthesis of Clary sage lactone has very high value.Currently, domestic close
What is generallyd use at Clary sage lactone is chemical synthesis, needs to aoxidize to obtain by sclareol with oxidant potassium permanganate etc..
In this process, the use of a large amount of oxidant and catalyst can cause very big harm to environment, and chemical method synthesis
Clary sage lactone is used for food, and purity is not high enough, is used for cosmetics, fragrance component is easy impacted.
Summary of the invention
The purpose of the present invention is to provide a kind of are able to solve in chemical method synthesis Clary sage lactone technical process to occur
The method of the biosynthesis Clary sage lactone for the problems such as environment is unfriendly, complex steps, low safety.
In order to achieve the above objectives, the technical solution adopted by the present invention is that:
1) take yeast culture medium and be separately added into thereto yeast culture medium quality 3%~8% sclareol and 4%~
10% shallow white Cryptococcus plasm body fluid obtains mixture；
2) mixture is placed in glass container, then the shake flask fermentation on 23-28 DEG C, the shaking table of 140~180r/min
Obtain Clary sage lactone within 10 days.
The pH value of the yeast culture medium is 6.0.
The Tween 80 of its quality 1.5%~4% is additionally added in the yeast culture medium.
The shallow white Cryptococcus is through 3~4min of ultraviolet mutagenesis.
The present invention utilizes microbe transformation method, using sclareol as substrate, is turned using shallow white Cryptococcus
Change prepares Clary sage lactone, solve the unfriendly environment occurred in chemical method synthesis Clary sage lactone technical process, complex steps,
The problems such as safety is low provides new way for the synthesis of Clary sage lactone.
Specific embodiment
Embodiment 1:
1) take yeast culture medium and be separately added into thereto yeast culture medium quality 3% sclareol and 6% it is shallow white
Color Cryptococcus plasm body fluid obtains mixture；
2) mixture is placed in glass container, is then obtained within shake flask fermentation 10 days on 25 DEG C, the shaking table of 140r/min
Clary sage lactone.
Embodiment 2:
1) take pH value be 6.0 yeast culture medium and be separately added into thereto yeast culture medium quality 5% sclareol,
1.5% Tween 80 and the 4% shallow white Cryptococcus plasm body fluid through ultraviolet mutagenesis 3min obtain mixture；
2) mixture is placed in glass container, is then obtained within shake flask fermentation 10 days on 23 DEG C, the shaking table of 160r/min
Clary sage lactone.
Embodiment 3:
1) take pH value be 6.0 yeast culture medium and be separately added into thereto yeast culture medium quality 8% sclareol,
3% Tween 80 and the 8% shallow white Cryptococcus plasm body fluid through ultraviolet mutagenesis 4min obtain mixture；
2) mixture is placed in glass container, is then obtained within shake flask fermentation 10 days on 26 DEG C, the shaking table of 150r/min
Clary sage lactone.
Embodiment 4:
1) take pH value be 6.0 yeast culture medium and be separately added into thereto yeast culture medium quality 6% sclareol,
4% Tween 80 and the 10% shallow white Cryptococcus plasm body fluid through ultraviolet mutagenesis 3.5min obtain mixture；
2) mixture is placed in glass container, is then obtained within shake flask fermentation 10 days on 28 DEG C, the shaking table of 180r/min
Clary sage lactone.
The present invention passes through ultraviolet mutagenesis shallowly white Cryptococcus, the bacterial strain of breeding high-yield Clary sage lactone.By embodiment 4
Preparation method, the yield highest of Clary sage lactone, be 35.2g/L.By to shallow white Cryptococcus ultraviolet mutagenesis processing 3
~4min, obtains two plants of Clary sage lactone high-yielding mutants, and yield improves 18.2% than starting strain respectively.
Claims (4)
1. a kind of method of biosynthesis Clary sage lactone, it is characterised in that the following steps are included:
1) it takes yeast culture medium and is separately added into the sclareol and 4%~10% of yeast culture medium quality 3%~8% thereto
Shallow white Cryptococcus plasm body fluid obtain mixture；
2) mixture is placed in glass container, then shake flask fermentation 10 days on 23-28 DEG C, the shaking table of 140~180r/min
Obtain Clary sage lactone.
2. the method for biosynthesis Clary sage lactone according to claim 1, it is characterised in that: the yeast culture medium
PH value be 6.0.
3. the method for biosynthesis Clary sage lactone according to claim 1, it is characterised in that: the yeast culture medium
In be additionally added the Tween 80 of its quality 1.5%~4%.
4. the method for biosynthesis Clary sage lactone according to claim 1, it is characterised in that: the shallow hidden ball of white
Yeast is through 3~4min of ultraviolet mutagenesis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910275371.3A CN109825539A (en) | 2019-04-08 | 2019-04-08 | A kind of method of biosynthesis Clary sage lactone |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910275371.3A CN109825539A (en) | 2019-04-08 | 2019-04-08 | A kind of method of biosynthesis Clary sage lactone |
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| CN109825539A true CN109825539A (en) | 2019-05-31 |
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| CN201910275371.3A Pending CN109825539A (en) | 2019-04-08 | 2019-04-08 | A kind of method of biosynthesis Clary sage lactone |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646737A (en) * | 2021-01-22 | 2021-04-13 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | Cryptococcus albidus strain YN14 capable of producing aroma substances at high yield and application thereof |
| CN113293106A (en) * | 2021-07-12 | 2021-08-24 | 江南大学 | Fungus of genus Filobasidium of class Ascomycetes and application thereof |
| CN113502306A (en) * | 2021-07-12 | 2021-10-15 | 江南大学 | Method for producing sclareolide by catalyzing sclareol |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4970163A (en) * | 1989-08-28 | 1990-11-13 | International Flavors & Fragrances Inc. | Process for producing diol and lactone and microorganisms capable of same |
| EP2546244A1 (en) * | 2011-07-13 | 2013-01-16 | Koste Biochemicals | Supercritical process for manufacturing ambradiol, sclareolide and (-)-ambrafuran from sclareol |
-
2019
- 2019-04-08 CN CN201910275371.3A patent/CN109825539A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4970163A (en) * | 1989-08-28 | 1990-11-13 | International Flavors & Fragrances Inc. | Process for producing diol and lactone and microorganisms capable of same |
| EP2546244A1 (en) * | 2011-07-13 | 2013-01-16 | Koste Biochemicals | Supercritical process for manufacturing ambradiol, sclareolide and (-)-ambrafuran from sclareol |
Non-Patent Citations (2)
| Title |
|---|
| MICHEL SCHALK等: "Toward a Biosynthetic Route to Sclareol and Amber Odorants", 《JOURNAL OF THE AMERICAN CHEMICAL SOCIETY》 * |
| 陈驰: "香紫苏醇合成降龙涎醚和香紫苏内酯的研究进展", 《天津化工》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646737A (en) * | 2021-01-22 | 2021-04-13 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | Cryptococcus albidus strain YN14 capable of producing aroma substances at high yield and application thereof |
| CN113293106A (en) * | 2021-07-12 | 2021-08-24 | 江南大学 | Fungus of genus Filobasidium of class Ascomycetes and application thereof |
| CN113502306A (en) * | 2021-07-12 | 2021-10-15 | 江南大学 | Method for producing sclareolide by catalyzing sclareol |
| CN113293106B (en) * | 2021-07-12 | 2022-09-09 | 江南大学 | Fungus of genus Filobasidium of class Ascomycetes and application thereof |
| CN113502306B (en) * | 2021-07-12 | 2023-06-23 | 江南大学 | Method for producing sclareolide by catalyzing sclareol |
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