CN109821668B - Centrifuge for separating wheat seedling ice structure protein and use method thereof - Google Patents

Abstract

The invention discloses a centrifuge for separating wheat seedling ice structure protein, which belongs to the technical field of centrifuges and comprises a machine body, wherein a display screen and a control panel are arranged at the upper end of the front side of the machine body, heat dissipation holes are formed in the side wall of the machine body, a cavity is rotatably connected in the machine body, a machine cover is hinged to the top of the machine body, a locking mechanism is arranged on the machine cover, a rotating head is installed in the cavity and comprises an outer barrel and an inner barrel, a first clamping groove, a first sliding groove and a second sliding groove are formed in the inner wall of the outer barrel, the bottom of the second sliding groove is connected with a spring and a support ring, a top disc is connected to the upper end of the outer; the upper end and the lower end of the inner cylinder are provided with internal threads, the bottom of the cavity is rotatably connected with a first screw, and the outer wall of the inner cylinder is connected with a first clamping block and a clamping ring. The invention can automatically adjust the weight of the symmetrical position on the rotating head, so that the rotation is more stable, and the centrifugal effect is improved.

Description

Centrifuge for separating wheat seedling ice structure protein and use method thereof

Technical Field

The invention belongs to the technical field of centrifuges, and particularly relates to a centrifuge for separating wheat seedling ice structure protein and a using method thereof.

Background

Unfrozen protein, also known as ice structuring protein or ice histon (ISP), acts to prevent the formation of ice crystals and to minimize the growth of ice crystals. At present, the effect of controlling the size of ice crystals by ISP is being applied to ice cream production, and even at very low concentrations of ISP, ice crystals in ice cream or confectionery can be inhibited from growing and the shape of the ice crystals can be variously changed. Class 5 ISPs distributed in plants, marine fish, insects and microorganisms have now been discovered and more ISPs of different origin have been discovered and identified. Due to the limited freezing point depression range and small production volumes of ISP, its high cost limits its widespread use in food products compared to traditional edible antifreeze agents. The wheat seedling is a cheap and easily-obtained plant and can generate ISP (internet service provider) under the induction of low-temperature stress. In the experimental process, in order to separate the ice structural protein of the wheat seedlings, a high-speed freezing centrifuge is required, the rotating speed of the high-speed freezing centrifuge can reach more than 10000rpm, the high-speed freezing centrifuge generally comprises a shell, a centrifugal chamber, a rotating head and a freezing device, wherein the rotating head is mostly made of titanium alloy or aluminum alloy.

When the existing high-speed refrigerated centrifuge is used, a plurality of symmetrical test tubes can be arranged on a rotating head, in order to enable the rotation to be stable, the total weight of each test tube and the liquid filled in the test tube must be almost equal, so that an experimenter must accurately weigh the weight of the liquid needing centrifugation, and the operation difficulty is improved.

Disclosure of Invention

The invention aims to: the centrifugal machine for separating the ice structure protein of the wheat seedlings is provided, and during centrifugation, the weight of the symmetrical positions on the rotating head can be automatically adjusted, so that the rotation is more stable, and the centrifugation effect is improved.

In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:

the centrifuge for separating wheat seedling ice structure protein comprises a machine body, a display screen and a control panel are arranged at the upper end of the front side of the machine body, the side wall of the machine body is provided with heat dissipation holes, a cavity is arranged in the machine body, the top of the machine body is hinged with a machine cover, the cover is provided with a locking mechanism, a rotating head is arranged in the cavity and comprises an outer cylinder and an inner cylinder, the outer barrel is of a hollow structure, the inner wall of the outer barrel is provided with a first clamping groove, the lower end of the first clamping groove is connected with a first sliding groove, a second sliding groove is arranged on the inner wall of the outer barrel below the first sliding groove, the bottom of the second sliding groove is connected with a spring, the upper end of the spring is connected with a support ring, the upper end of the outer barrel is connected with a top disc, the bottom of the top disc is connected with at least two symmetrically arranged hanging plates, a balance mechanism is installed on each hanging plate, and a test tube sleeve is hung on each balance mechanism;

the upper end and the lower end of the inner cylinder are respectively provided with an internal thread, the bottom of the cavity is rotatably connected with a first screw, the outer wall of the inner cylinder is connected with a first clamping block and a clamping ring, the clamping ring is located below the first clamping block, and the clamping ring is rotatably connected in the second sliding groove and located on the support ring.

When the device is used, firstly, six parts of ultralow-temperature wheat seedling extracting solution with equal weight are weighed and loaded into each test tube, then, each test tube is sequentially placed into the corresponding tube sleeve, the test tube sleeves are hung on the corresponding hanging blocks, then, the rotating head is placed into the cavity through the lifting and placing mechanism and is connected with the first screw rod, the lifting and placing mechanism is taken down, the cover is closed, the cover is locked through the locking mechanism, then, the motor is started, the motor drives the rotating head to rotate at high speed, the ultralow-temperature wheat seedling extracting solution is centrifuged, and after the centrifugation is finished, the motor is closed, and the test tubes are taken out.

Further inject, the junction of first draw-in groove and first spout is equipped with the inclined plane structure, also be equipped with the inclined plane structure on the first fixture block. The inclined plane structure is convenient for the first clamping block to slide into the first clamping groove from the first sliding groove, and the guiding effect is good.

Further, the number of the first clamping blocks and the number of the first clamping grooves are six. When the clamping device is installed, the first clamping block can conveniently slide into the first clamping groove.

Further inject, balance mechanism includes the link block again, link block sliding connection is in the link plate that the symmetry set up, still be equipped with the sliding frame in the link plate, link block afterbody sliding connection is in the sliding frame, be equipped with the liquid bag between link block afterbody and the sliding frame inner wall, be connected with the liquid pipe between the liquid bag of symmetry, the liquid pipe passes in link plate and the overhead pan. During centrifugation, the test tube cover becomes the horizontal direction by vertical direction, the centrifugal force that the heavier test tube cover of weight received is great, make the outside motion of test tube cover, the hanger outwards slides on the link plate, the hanger extrudees the liquid bag, pass through in the liquid bag of liquid pipe inflow symmetric position with the liquid in the liquid bag, thereby reduce the weight of this side liquid bag interior liquid, increase the weight of the interior liquid of symmetry side liquid bag, make the mass distribution who turns round even, thereby play better centrifugation to the ultralow temperature wheat seedling extract in the test tube

Further inject, locking mechanism includes dwang and lockhole again, the dwang rotates to be connected on the cover, and is connected with the torsional spring between dwang and the cover, the dwang upper end is connected with the knob, be connected with the second fixture block on the lateral wall of dwang lower extreme, the lockhole is seted up on the organism, open the lockhole bottom has the second draw-in groove. During locking, two knobs are respectively held by two hands, the knobs are rotated to enable the second fixture block to rotate 90 degrees, then the cover is covered, the rotating rod is inserted into the locking hole, then the knobs are released, the two rotating rods reset under the action of the torsional spring, and the second fixture block is clamped into the second clamping groove, so that the cover is locked.

Further limit, the number of the hanging plates is six. Thus, the ultralow-temperature wheat seedling extracting solutions in six test tubes can be centrifuged at one time, and the working efficiency is improved.

Further inject, the test tube box includes the tube cap again, the tube cap top is connected with the link, the tube cap bottom is connected with the boss, the boss outer wall is equipped with the external screw thread, boss threaded connection has the pipe box. Such structure, easy operation, simple to operate can play better guard action to the test tube.

Further inject, still including carrying and putting the mechanism, carry and put the mechanism and include the handle again, be connected with the soft cover on the handle, be connected with the stock on the handle, the stock lower extreme is connected with and keeps off the ring, it is connected with the second screw rod to keep off the ring bottom, the internal thread of both ends revolves to the homogeneous phase about second screw rod, first screw rod and inner tube. Through carrying put the mechanism, can comparatively conveniently mention the bull head and be connected it with first screw rod, and need not directly to carry the bull head with the hand, convenient operation.

The invention also provides a use method of the centrifuge for separating the wheat seedling ice structure protein, which mainly comprises the following steps:

step one, installation.

Firstly, filling equal amounts of ultralow-temperature wheat seedling extracting solution into each test tube, then sequentially putting each test tube into a corresponding tube sleeve, connecting a tube cover to the tube sleeve through threaded connection, and then hanging each test tube sleeve on a hanging block through a hanging ring;

then, connecting a second screw rod with the internal thread at the upper end of the inner cylinder until the baffle ring abuts against the upper end of the inner cylinder, and placing the rotating head into the cavity through the lifting mechanism to enable the internal thread at the lower end of the inner cylinder to be positioned on the first screw rod;

then the handle is pressed, the outer barrel is lifted upwards, the clamping ring presses the support ring downwards, meanwhile, the spring is compressed, the first clamping block slides downwards to the first sliding groove from the first clamping groove, then the handle is rotated, the first clamping block slides in the first sliding groove, the clamping ring rotates on the support ring, meanwhile, the inner thread at the lower end of the inner barrel is connected with the first screw, the handle continues to be rotated until the inner thread at the lower end of the inner barrel is completely connected with the first screw, the outer barrel is released, the outer barrel moves downwards relative to the inner barrel under the action of the spring, the bottom of the outer barrel abuts against the bottom of the cavity, the first clamping block is clamped into the first clamping groove again, then the handle is rotated reversely, the second screw is taken out of the inner thread.

And step two, locking.

Two knobs are held by two hands respectively, the knobs are rotated to enable the second fixture blocks to rotate 90 degrees, then the cover is covered, the rotating rods are inserted into the locking holes, then the knobs are released, the two rotating rods reset under the action of the torsional springs, and the second fixture blocks are clamped into the second clamping grooves, so that the cover is locked.

And step three, centrifuging.

Start the motor in the organism, the motor drives through first screw rod and turns round high-speed the rotation, the test tube cover becomes the horizontal direction by vertical orientation, the test tube cover, the centrifugal force that the heavier one side of total weight of test tube and ultra-low temperature wheat seedling extract received is great, make the test tube cover outwards move, the hanging block outwards slides on the link plate, hanging block extrusion liquid bag, pass through in the liquid bag of liquid pipe inflow symmetric position with liquid in the liquid bag, thereby reduce the weight of liquid in this side liquid bag, increase the weight of liquid in the liquid bag of symmetry side, make turn round and turn round the whole mass distribution that the article that hangs constitute more even, thereby play better centrifugal action to the ultra-low temperature wheat seedling extract in the test tube.

And step four, disassembling.

And after the centrifugal process is finished, the motor is turned off, and the test tube is taken out according to the steps opposite to the steps.

Compared with the prior art, the invention mainly has the following advantages:

1. the balance mechanism is arranged, so that the mass distribution of the rotary head can be automatically adjusted, the rotating stability is improved, the centrifugal effect is enhanced, and the operation difficulty of experimenters is reduced;

2. the cover can be quickly locked by the locking mechanism, and the operation is simple and convenient.

Drawings

The invention is further illustrated by the non-limiting examples given in the accompanying drawings;

FIG. 1 is a schematic structural view of a lid closed according to an embodiment of the present invention;

FIG. 2 is a first schematic structural diagram illustrating the opening of the lid according to the embodiment of the present invention;

FIG. 3 is a second structural diagram illustrating the opening of the lid according to the embodiment of the present invention;

FIG. 4 is a schematic view, partly in section, of a locking mechanism in an embodiment of the invention;

FIG. 5 is a schematic view of a connection structure between a rotor and a test tube sleeve according to an embodiment of the present invention;

FIG. 6 is a schematic view of a partial cross-sectional structure of a rotor connected to a test tube sleeve according to an embodiment of the present invention;

FIG. 7 is a schematic view of a portion of the structure of FIG. 6 at A;

FIG. 8 is a schematic structural diagram of a test tube holder according to an embodiment of the present invention;

FIG. 9 is a partial sectional view schematically showing the outer tub in the embodiment of the present invention;

FIG. 10 is a schematic view of a portion of the structure of FIG. 9 at B;

FIG. 11 is a schematic structural view of an inner barrel in an embodiment of the present invention;

FIG. 12 is a schematic structural diagram of a lifting mechanism according to an embodiment of the present invention;

the main element symbols are as follows:

the liquid crystal display comprises a machine body 1, a display screen 11, a control panel 12, a heat dissipation hole 13, a first screw rod 14, a cavity 15, a machine cover 2, a rotary head 3, an outer cylinder 31, a first clamping groove 32, a first sliding groove 33, a second sliding groove 34, a spring 35, a support ring 36, a top plate 37, a hanging plate 38, an inner cylinder 4, a first clamping block 41, a clamping ring 42, a hanging block 51, a sliding frame 52, a liquid bag 53, a liquid pipe 54, a pipe cover 61, a hanging ring 62, a boss 63, a pipe sleeve 64, a rotating rod 71, a locking hole 72, a knob 73, a second clamping block 74, a second clamping groove 75, a handle 81, a long rod 82, a retaining ring 83 and a second.

Detailed Description

In order that those skilled in the art can better understand the present invention, the following technical solutions are further described with reference to the accompanying drawings and examples.

As shown in fig. 1 to 12, the centrifuge for separating ice structural protein of wheat seedlings according to the present invention is the same as the existing centrifuge in that: including organism 1, 1 front side upper end of organism is equipped with display screen 11 and the control panel 12 that is equipped with operating button that shows operational data such as temperature, rotational speed, is equipped with louvre 13 on the 1 lateral wall of organism, is equipped with cavity 15 in the organism 1, and 1 top of organism articulates there is cover 2, installs the turn round 3 in the cavity 15.

The centrifuge of the present invention is different from the existing centrifuge in that: in order to install the test tube, the test tube sleeve is hung on the rotary head 3 and comprises a tube cover 61, the top of the tube cover 61 is connected with a hanging ring 62, the bottom of the tube cover 61 is connected with a boss 63, the outer wall of the boss 63 is provided with an external thread, the top of the tube sleeve 64 is provided with an internal thread, the boss 63 is in threaded connection with the tube sleeve 64, and the inner diameter of the tube sleeve 64 is just matched with the outer diameter of the test tube.

Before the experiment, the same amount of ultralow-temperature wheat seedling extracting solution is weighed and filled into each test tube, then each test tube is sequentially placed into the corresponding tube sleeve 64, and the tube cover 61 is connected onto the tube sleeve 64 through threaded connection.

The rotary head 3 comprises an outer cylinder 31 and an inner cylinder 4 which are of a hollow structure, the inner cylinder 4 is installed in the outer cylinder 31, in order to hang the test tube sleeve on the rotary head 3, the upper end of the outer cylinder 31 is connected with a top disc 37 of a disc structure, and the bottom of the top disc 37 is connected with six symmetrically-arranged hanging plates 38 used for hanging the test tube sleeve along the circumferential direction.

In order to carry the rotor 3 conveniently, the invention further comprises a lifting mechanism, the lifting mechanism further comprises a handle 81, the handle 81 is connected with a soft sleeve, the handle 81 is connected with a long rod 82, the lower end of the long rod 82 is connected with a baffle ring 83, the bottom of the baffle ring 83 is connected with a second screw 84, the upper end of the inner cylinder 4 is provided with an internal thread, and the rotating directions of the second screw 84 and the internal thread at the upper end of the inner cylinder 4 are the same. When the rotary head is carried, the upper end of the inner cylinder 4 is slightly lower than the upper end of the outer cylinder 31, the second screw 84 is connected with the internal thread at the upper end of the inner cylinder 4 through threads until the stop ring 83 abuts against the upper end of the inner cylinder 4, and then the whole rotary head 3 can be lifted up by lifting the handle 81 without directly contacting the rotary head 3 with hands, so that the rotary head is convenient to carry.

In order to connect the rotor 3 with the cavity 15, the bottom of the cavity 15 is rotatably connected with a first screw 14, the inner wall of the outer cylinder 31 is provided with six first clamping grooves 32, the lower end of each first clamping groove 32 is connected with a first sliding chute 33 with an annular structure, the inner wall of the outer cylinder 31 is provided with a second sliding chute 34 below the first sliding chute 33, the second sliding chute 34 is also in an annular structure, the bottom of the second sliding chute 34 is connected with a spring 35, the upper end of the spring 35 is connected with a support ring 36, in order to match the inner cylinder 4 with the outer cylinder 31, six first clamping blocks 41 and clamping rings 42 are connected on the outer wall of the inner cylinder 4, wherein, the first clipping block 41 can be just clipped into the first clipping groove 32, the clipping ring 42 is rotationally connected in the second sliding groove 34 and is positioned on the supporting ring 36, in addition, the thickness of the snap ring 42 is smaller than the height of the second sliding groove 34, the lower end of the inner cylinder 4 is provided with internal threads, the screwing directions of the internal threads at the upper end and the lower end of the inner cylinder 4 are the same, and the screwing direction of the internal threads at the lower end of the inner cylinder 4 is the same as that of the first screw 14.

When the device is installed, the rotating head 3 is placed into the cavity 15 through the lifting mechanism, the lower end of the inner cylinder 4 is lower than the lower end of the outer cylinder 31, the internal thread at the lower end of the inner cylinder 4 is positioned on the first screw 14, then the handle 81 is pressed, the outer cylinder 31 is lifted up slightly by hand, the snap ring 42 presses down the support ring 36, the spring 35 is compressed, the first fixture block 41 slides down from the first clamping groove 32 to the first sliding groove 33, then the handle 81 is rotated, the second screw 84 drives the inner cylinder 4 to rotate in the outer cylinder 31, the first fixture block 41 slides in the first sliding groove 33, the snap ring 42 rotates on the support ring 36, the internal thread at the lower end of the inner cylinder 4 is connected with the first screw 14, the handle 81 is rotated continuously until the internal thread at the lower end of the inner cylinder 4 is completely connected with the first screw 14, the outer cylinder 31 is released, the outer cylinder 31 moves downwards relative to the inner cylinder 4 under the, first fixture block 41 is blocked in first draw-in groove 32 again, in this embodiment, in order to make first fixture block 41 block first draw-in groove 32 better, first draw-in groove 32 is equipped with the inclined plane structure with the junction of first spout 33, also be equipped with the inclined plane structure on first fixture block 41, the inclined plane structure direction is effectual, thereby make things convenient for first fixture block 41 to block in first draw-in groove 32, then reverse rotation handle 81, because inner tube 4 is blocked by urceolus 31, so inner tube 4 can not rotate, thereby make second screw 84 take out from the internal thread of inner tube 4 upper end, accomplish the installation.

After the installation is accomplished, close cover 2, in order to lock cover 2, be equipped with locking mechanism on cover 2, locking mechanism includes dwang 71 and lockhole 72 again, dwang 71 rotates to be connected on cover 2, and be connected with the torsional spring (not drawn in the picture) between dwang 71 and the cover 2, the torsional spring can play the effect that resets to dwang 71, dwang 71 upper end is connected with knob 73, be connected with second fixture block 74 on the lateral wall of dwang 71 lower extreme, lockhole 72 is seted up on organism 1, open lockhole 72 bottom has second draw-in groove 75.

During locking, two knobs 73 are respectively held by two hands, the knobs 73 are rotated, the second fixture blocks 74 are rotated for 90 degrees (namely, the left knob 73 rotates for 90 degrees in the anticlockwise direction and the right knob 73 rotates for 90 degrees in the clockwise direction in the view of fig. 2), the positions of the second fixture blocks 74 are just matched with the locking holes 72, then the cover 2 is covered, the rotating rods 71 are inserted into the locking holes 72, then the knobs 73 are released, under the action of the torsion springs, the two rotating rods 71 are reset, the second fixture blocks 74 are clamped into the second clamping grooves 75, and therefore the cover 2 is locked.

During centrifugation, a motor in the machine body 1 is started, and the motor drives the first screw 14 to rotate through a transmission mechanism, which is the prior art and is not described herein again. The first screw 14 drives the rotary head 3 to rotate at a high speed, the test tube sleeve is changed from the vertical direction to the horizontal direction, and after the test tube sleeve runs for a proper time, the ultralow-temperature wheat seedling extracting solution in the test tube can be separated.

During the separation, in order to make the mass distribution of bull head 3 even, have better centrifugal action, install balance mechanism on link plate 38, balance mechanism includes link block 51 again, link block 51 sliding connection is in link plate 38, still be equipped with sliding frame 52 in link plate 38, link block 51 afterbody sliding connection is in sliding frame 52, be equipped with liquid bag 53 between link block 51 afterbody and the sliding frame 52 inner wall, be connected with liquid pipe 54 between symmetrical liquid bag 53, liquid pipe 54 passes in link plate 38 and the overhead pan 37, liquid pipe 54 at overhead pan 37 middle part is the annular, can make liquid pipe 54 bypass inner tube 4 like this, avoid taking place the interference.

During centrifugation, the test tube sleeve is changed from the vertical direction to the horizontal direction, and the calculation formula F of the centrifugal force is mrW²It can be seen that, when the angular velocity and radius are constant, the larger the mass is, the larger the centrifugal force is, and when weighing is performed, the total weight of the ultralow temperature wheat seedling extracting solution in each test tube and each test tube cannot be completely equal to each other, so that the condition that the ultralow temperature wheat seedling extracting solution in each test tube is completely equal to the total weight of the ultralow temperature wheat seedling extracting solution in each testCertain errors, together with the weight of each test tube sleeve, may not be completely equal, so that during rotation, the side with the heavier total weight of the test tube sleeve, the test tube and the ultralow temperature wheat seedling extract is subjected to larger centrifugal force, so that the test tube sleeve moves outwards, the hanging block 51 slides outwards on the hanging plate 38, the hanging block 51 extrudes the liquid bag 53, the liquid in the liquid bag 53 flows into the liquid bags 53 at symmetrical positions through the liquid pipe 54, thereby reducing the weight of the liquid in the side liquid sac 53 and increasing the weight of the liquid in the symmetrical side liquid sac 53, because the weight difference of the ultra-low temperature wheat seedling extracting solution in each test tube is not very large, the weight of the transferred liquid is enough to make up the weight difference, so that the whole mass distribution formed by the rotary head 3 and the articles hung on the rotary head 3 is more uniform, and the ultra-low temperature wheat seedling extracting solution in the test tube has better centrifugation effect.

The invention also provides a use method of the centrifuge for separating the ice structure protein of the wheat seedling, before the experiment, 150g of the ultralow temperature wheat seedling is weighed, 600ML precooling buffer solution 25mmol/LTris-HCL, pH7.8,0.1mmol/LEDTA,1mmol/L PMSF and 0.1mmol/LKCL are added, and stirring and crushing are carried out to obtain the ultralow temperature wheat seedling extracting solution. During the experiment, the method mainly comprises the following steps:

step one, installation.

Firstly, filling the substantially equal amount of ultralow-temperature wheat seedling extracting solution into each test tube, then sequentially putting each test tube into the corresponding tube sleeve 64, connecting the tube cover 61 to the tube sleeve 64 through threaded connection, and then hanging each test tube on the hanging block 51 through the hanging ring 62;

then, the second screw rod 84 is connected with the internal thread at the upper end of the inner cylinder 4 through threads until the retaining ring 83 abuts against the upper end of the inner cylinder 4, the rotating head 3 is lifted through the lifting mechanism, and the rotating head 3 is placed in the cavity 15, so that the internal thread at the lower end of the inner cylinder 4 is positioned on the first screw rod 14;

then the handle 81 is pressed, the outer cylinder 31 is lifted upwards, the snap ring 42 presses the support ring 36 downwards, at the same time, the spring 35 is compressed, and the first latch 41 slides down from the first latch groove 32 to the first slide groove 33, then, the handle 81 is rotated, the second screw 84 drives the inner cylinder 4 to rotate in the outer cylinder 31, so that the first fixture block 41 slides in the first sliding slot 33, the clamp ring 42 rotates on the support ring 36, while the internal thread of the lower end of the inner cylinder 4 is connected to the first screw 14, the handle 81 is continuously rotated until the internal thread of the lower end of the inner cylinder 4 is completely connected to the first screw 14, the outer cylinder 31 is released, under the action of the spring 35, the outer cylinder 31 moves downward relative to the inner cylinder 4, the bottom of the outer cylinder 31 abuts against the bottom of the cavity 15, the first latch 41 is re-latched in the first latch groove 32, then, the handle 81 is rotated in the reverse direction to remove the second screw 84 from the internal thread at the upper end of the inner cylinder 4, thereby completing the installation.

And step two, locking.

Two knobs 73 are respectively held by two hands, the knobs 73 are rotated to enable the second fixture blocks 74 to rotate 90 degrees, then the cover 2 is covered, the rotating rods 71 are inserted into the lock holes 72, then the knobs 73 are released, the two rotating rods 71 reset under the action of the torsion springs, and the second fixture blocks 74 are clamped into the second clamping grooves 75, so that the cover 2 is locked.

And step three, centrifuging.

The motor in the starter body 1, the motor passes through first screw rod 14 and drives the high-speed rotation of bull 3, the test tube cover becomes the horizontal direction by vertical direction, the test tube cover, the centrifugal force that the heavier one side of total weight of test tube and ultra-low temperature wheat seedling extract received is great, make the test tube cover outwards move, hanger 51 outwards slides on link plate 38, hanger 51 extrusion liquid bag 53, pass through liquid pipe 54 with the liquid in the liquid bag 53 and flow into the liquid bag 53 of symmetric position, thereby reduce the weight of liquid in this side liquid bag 53, increase the weight of liquid in the symmetrical side liquid bag 53, make the holistic mass distribution that article that hang on bull 3 and the bull 3 constitute more even, thereby play better centrifugal action to the ultra-low temperature wheat seedling extract in the test tube.

And step four, disassembling.

And after the centrifugal process is finished, the motor is turned off, and the test tube is taken out according to the steps opposite to the steps.

The centrifuge for separating ice structural protein of wheat seedlings and the use method thereof provided by the invention are described in detail above. The description of the specific embodiments is only intended to facilitate an understanding of the method of the invention and its core ideas. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.

Claims (10)

1. A centrifuge for wheat seedling ice structure protein separation, including organism (1), organism (1) front side upper end is equipped with display screen (11) and control panel (12), be equipped with louvre (13) on organism (1) lateral wall, be equipped with cavity (15) in organism (1), organism (1) top articulates there is cover (2), install in cavity (15) and turn round (3), its characterized in that: the rotating head (3) comprises an outer barrel (31) and an inner barrel (4), the outer barrel (31) is of a hollow structure, a first clamping groove (32) is formed in the inner wall of the outer barrel (31), the lower end of the first clamping groove (32) is connected with a first sliding groove (33), a second sliding groove (34) is formed in the inner wall of the outer barrel (31) below the first sliding groove (33), the bottom of the second sliding groove (34) is connected with a spring (35), the upper end of the spring (35) is connected with a support ring (36), the upper end of the outer barrel (31) is connected with a top disc (37), the bottom of the top disc (37) is connected with at least two symmetrically-arranged hanging plates (38), a balance mechanism is installed on each hanging plate (38), a test tube sleeve is hung on the balance mechanism, and a locking mechanism is arranged on the cover (;

the upper end and the lower end of the inner cylinder (4) are respectively provided with an internal thread, the bottom of the cavity (15) is rotatably connected with a first screw (14), the outer wall of the inner cylinder (4) is connected with a first clamping block (41) and a clamping ring (42), the clamping ring (42) is located below the first clamping block (41), and the clamping ring (42) is rotatably connected in the second sliding groove (34) and located on the support ring (36).

2. The centrifuge for separation of ice structural protein of wheat seedlings according to claim 1, wherein: the connecting part of the first clamping groove (32) and the first sliding groove (33) is provided with an inclined plane structure, and the first clamping block (41) is also provided with an inclined plane structure.

3. The centrifuge for separation of ice structural protein of wheat seedlings according to claim 1, wherein: the number of the first clamping blocks (41) and the number of the first clamping grooves (32) are six.

4. The centrifuge for separation of ice structural protein of wheat seedlings according to claim 1, wherein: balance mechanism includes link block (51) again, link block (51) sliding connection is in link plate (38) that the symmetry set up, still be equipped with sliding frame (52) in link plate (38), link block (51) afterbody sliding connection is in sliding frame (52), be equipped with liquid bag (53) between link block (51) afterbody and sliding frame (52) inner wall, be connected with liquid pipe (54) between liquid bag (53) of symmetry, pass in link plate (38) and head dish (37) liquid pipe (54).

5. The centrifuge for separation of ice structural protein of wheat seedlings according to claim 1, wherein: locking mechanism includes dwang (71) and lockhole (72) again, dwang (71) are rotated and are connected on cover (2), and are connected with the torsional spring between dwang (71) and cover (2), dwang (71) upper end is connected with knob (73), be connected with second fixture block (74) on the lateral wall of dwang (71) lower extreme, set up on organism (1) lockhole (72), lockhole (72) bottom is opened there is second draw-in groove (75).

6. The centrifuge for separation of ice structural protein of wheat seedlings according to claim 1, wherein: the number of the hanging plates (38) is six.

7. The centrifuge for separation of ice structural protein of wheat seedlings according to claim 1, wherein: the test tube sleeve comprises a tube cover (61), the top of the tube cover (61) is connected with a hanging ring (62), the bottom of the tube cover (61) is connected with a boss (63), the outer wall of the boss (63) is provided with external threads, and the boss (63) is in threaded connection with a tube sleeve (64).

8. The centrifuge for separation of ice structural protein of wheat seedlings according to any one of claims 1 to 7, wherein: still including carrying and putting the mechanism, carry and put the mechanism and include handle (81) again, be connected with stock (82) on handle (81), stock (82) lower extreme is connected with and keeps off ring (83), keep off ring (83) bottom and be connected with second screw rod (84), the internal screw thread at both ends revolves to the homogeneous phase about second screw rod (84), first screw rod (14) and inner tube (4).

9. The centrifuge for separation of ice structural protein of wheat seedlings according to claim 8, wherein: the handle (81) is connected with a soft sleeve.

10. The use method of the centrifuge for the separation of ice structural protein of wheat seedlings according to claim 9, wherein: mainly comprises the following steps of (1) carrying out,

step one, installation;

firstly, filling equal amounts of ultralow-temperature wheat seedling extracting solution into each test tube, then sequentially putting each test tube into a corresponding tube sleeve (64), connecting a tube cover (61) to the tube sleeve (64) through threaded connection, and then hanging each test tube on a hanging block (51) through a hanging ring (62);

then, a second screw rod (84) is connected with the inner cylinder (4), the rotating head (3) is placed into the cavity (15) through a lifting mechanism, and the lower end of the inner cylinder (4) is positioned on the first screw rod (14);

then the handle (81) is pressed, the outer cylinder (31) is lifted upwards, then the handle (81) is rotated, the second screw (84) drives the inner cylinder (4) to rotate in the outer cylinder (31) until the internal thread at the lower end of the inner cylinder (4) is completely connected with the first screw (14), the outer cylinder (31) is released, the outer cylinder (31) moves downwards relative to the inner cylinder (4) under the action of the spring (35), the bottom of the outer cylinder (31) abuts against the bottom of the cavity (15), then the handle (81) is rotated reversely, and the second screw (84) is taken out of the internal thread at the upper end of the inner cylinder (4) to complete installation;

step two, locking;

two knobs (73) are respectively held by two hands, the knobs (73) rotate for 90 degrees, then the cover (2) is covered, the rotating rods (71) are inserted into the lock holes (72), then the knobs (73) are released, the two rotating rods (71) reset under the action of the torsion springs, the second clamping blocks (74) are clamped into the second clamping grooves (75), and the cover (2) is locked;

step three, centrifuging;

starting a motor in the machine body (1), wherein the motor drives the rotary head (3) to rotate at a high speed through the first screw (14), the test tube sleeve is changed from the vertical direction to the horizontal direction, the centrifugal force on the heavier side of the total weight of the test tube sleeve, the test tube and the ultralow-temperature wheat seedling extracting solution is larger, so that the test tube sleeve and the hanging block (51) slide outwards, the hanging block (51) extrudes the liquid bag (53), the liquid in the liquid bag (53) flows into the liquid bag (53) at the symmetrical position through the liquid pipe (54), the whole mass distribution formed by the rotary head (3) and articles hung on the rotary head (3) is more uniform, and the ultralow-temperature wheat seedling extracting solution in the test tube is better centrifuged;

step four, disassembling;

and after the centrifugal process is finished, the motor is turned off, and the test tube is taken out according to the steps opposite to the steps.

Images