CN109813889A - The time-resolved fluoroimmunoassay for detecting progesterone chromatographs quantitative testing test paper item - Google Patents
The time-resolved fluoroimmunoassay for detecting progesterone chromatographs quantitative testing test paper item Download PDFInfo
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- CN109813889A CN109813889A CN201711150978.6A CN201711150978A CN109813889A CN 109813889 A CN109813889 A CN 109813889A CN 201711150978 A CN201711150978 A CN 201711150978A CN 109813889 A CN109813889 A CN 109813889A
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Abstract
The present invention relates to progesterone rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper preparation method and applications.The test strips are made of Fusion5 film, nitrocellulose filter and blotting paper three parts, by competition law principle, using time-resolved fluorescence microballoon as immune marker, carry out quickly and accurately quantitative detection to determined antigen.
Description
Technical field
The invention belongs to field of detection of food safety, and in particular to the detection method of the Harmful Residue in food, especially
Time-resolved fluoroimmunoassay chromatograph test strip of progesterone quantitative detection and its preparation method and application.
Background technique
Progesterone is also known as progesterone, progesterone, corpus luteum sterone, corpus luteum hormone, progesterone, gestogen or to help progesterone be one
Kind it is related to menstrual cycle of female, gestation and on the mankind's also influential steroids of the embryo of other animals again.Progesterone is a kind of female
Sex hormone participates in the female menstrual cycle of the mankind and other animals, supports pregnancy and embry ogenesis.Progesterone is to belong to a kind of title
For the hormone of progestational hormone and the most important progestational hormone of the mankind.The feed conversion rate of poultry food can be improved in hormone medicine, in turn
Achieve the purpose that weight gain, but will lead to a series of problems because progesterone remains in vivo, if sex character changes, precocity etc., to children and
Teenager's effect becomes apparent.Currently, hormonal substance is forbidden using in aquaculture by most of country, and must not eat
It is detected in product.But domestic detection remaining to forbidden drugs such as progesterone in livestock products is almost blank, according to China to animal sources
Product residue monitoring regulation and the requirement in relation to importer, study various detection methods and have become a top priority.Currently, detection is pregnant
The method of ketone residual quantity is mainly high performance liquid chromatography (HPLC), due to complicated instrument and equipment and cumbersome process and right
The high professional qualification requirement of reviewer, is not suitable for the screening of on-site supervision and great amount of samples.
Current immunochromatography (lateral flow immunoassay, LFIA) Rapid detection test strip is mostly with colloid
Gold, color latex microballoon or fluorescein are as marker.Based on the quick testing product of colloidal gold-labeled method exploitation, exist
The problems such as qualitative or sxemiquantitative, differences between batches are larger；Although color latex microballoon difference between batch improves to some extent, sensitivity is still
It is lower, it also can only qualitative or sxemiquantitative；Immunochromatography sensitivity based on fluorescein labelling technique is greatly improved, also can be into
Row quantitative detection, but due to containing higher autofluorescent background signal in sample, and stock displacement is smaller, can generate to detection
Large effect.
Time-resolved fluorescence (Time-resolved Fluorescence, TRF) is a kind of heterotope fluorescent marker,
Compared with common fluorescent, have stock displacement big, the features such as fluorescence lifetime is long, can effectively avoid the background in sample glimmering
The influence of the stray lights such as light and exciting light, therefore there is higher sensitivity and anti-interference ability compared to common fluorescent.
The present invention substitutes traditional sample pad, blood filter membrane, bonding pad using Fusion5, by original four layers even five layers
Membranous system simplifies, and develops the fast quantification immunity chromatography detection test paper for only needing trilamellar membrane structure, not only obtains production technology
Simplification has been arrived, and has effectively reduced batch internal difference and the difference between batch of product, has improved detection sensitivity, great practical value.
Summary of the invention
The purpose of the invention is to provide novel, energy rapid quantitative detection progesterone time-resolved fluoroimmunoassay chromatography
Test strip and its preparation method and application.
One aspect of the present invention discloses progesterone time-resolved fluoroimmunoassay chromatography quantitative testing test paper item, which includes
Fusion5 film, nitrocellulose filter and blotting paper three parts.Nitrocellulose filter is located at centre, Fusion5 film and blotting paper
It is overlapped in nitrocellulose filter left and right ends respectively.Wherein, Fusion5 film is equipped with sample application zone and microballoon area, and microballoon area uploads
Having time resolved fluorometric microballoon；Nitrocellulose filter is equipped with detection line (T line) and nature controlling line (C line), and T line connects coating progesterone
Antigen, C line are coated with anti-rabbit antibody.
It is an advantage of the invention that the content of progesterone in tissue can be quickly detected.
The progesterone time-resolved fluorescence microballoon, including detection microballoon and Quality Control microballoon, detection microballoon have for pan coating
The fluorescent microsphere of progesterone monoclonal antibody, Quality Control microballoon are the fluorescent microsphere that pan coating has rabbit-anti labelled protein.
Bright-coloured series elements compound is filled in the fluorescent microsphere；Preferably, which is europium chelating object；
Optimal, which can be Eu(TTA)3/ TOPO or Eu(TTA)3/Phen 。
The albumen can be cow's serum Y-globulin (BGG) or balf serum albumin (BSA).
The time-resolved fluorescence microspherulite diameter range is 100-1000 nm.
On the nitrocellulose filter, coated antigen is progesterone antigen, coated anti-rabbit antibody on C line on T line.
The progesterone time-resolved fluoroimmunoassay quantitative testing test paper bottom is equipped with plastic bottom board.
Second aspect of the present invention discloses the preparation method of progesterone time-resolved fluoroimmunoassay chromatography quantitative testing test paper item,
The following steps are included:
(1) prepared by Quality Control microballoon:
1. using biotinylated protein；
2. being coated with aldehyde group modified fluorescent microsphere using above-mentioned labelled protein；
(2) detects microballoon preparation
Aldehyde group modified fluorescent microsphere is coated with using the monoclonal antibody of progesterone；
(3) blank kilocalorie is pasted
Using overlapped mode, first stickup nitrocellulose filter on the plastic bottom board with gum, then in cellulose nitrate
Blotting paper and Fusion5 film are pasted respectively in plain film left and right ends；
(4) sprays film
By the fluorescent microsphere of the fluorescent microsphere of rabbit-anti labelled protein and progesterone monoclonal antibody using release buffer mixed diluting to centainly
Concentration is sprayed onto the microballoon area of Fusion5 film；After progesterone antigen and the dilution of anti-rabbit antibody, it is sprayed onto the T line of nitrocellulose filter respectively
With C line position；
(5) is dry and cuts.
By the above-mentioned kilocalorie drying for having sprayed reagent, slitting.
Step (4) in the release buffer used contain 10-15% sucrose, 3-10% trehaloses, 0. 5-1%N, 0-
Double trimethylsilyl acetamides (BSA), 0.1 one 0.5% gentamicins.
Step detects final concentration of 0.5-3 mg/mL of microballoon, Quality Control microballoon final concentration 0.05-0.2 after (4) diluting
mg/mL；0.5-3 mg/mL of T line antigen final concentration, 0.5-23mg/mL of C line antigen final concentration；It is 0.5 that C, T line, which spray film liquid amount,
- 1.5 μ 1/cm, it is 2-10 μ l/cm that microballoon, which sprays film amount,.
Step (5) described in drying can in constant temperature oven or drying chamber, 37 DEG C dry 12-16 hours.
Third aspect present invention discloses the application of progesterone time-resolved fluoroimmunoassay chromatography quantitative testing test paper item.
Test strips of the invention can measure biomolecule by competition law principle, use competition law mode suitable for all
Immunochromatography detection.
Progesterone time-resolved fluoroimmunoassay chromatograph test strip high sensitivity of the invention, withinrun precision up to 10% or so,
Betweenrun precision can detect whole blood, serum, blood plasma, urine specimen, 250 mg/dL hemoglobins, 500 mg/ up to 15% simultaneously
DL triglyceride, 10 mg/dL bilirubin on the detection of this test strips without influence, it is fast far more than most of immunochromatographies in the market
Fast diagnostic products.
Detailed description of the invention
Fig. 1 test strips structure schematic diagram of the present invention (1. plastic bottom boards, 2. Fusion5 films, 3. nitrocellulose filters, 4.
Blotting paper, 5. sample application zones, 6. microballoon areas, 7. T line areas, 8. C line areas).
Specific embodiment
1. the preparation of each ingredient of test strips
The preparation for preparing the coated Quality Control microballoon of biotin labeling gamma globulin (BGG) of 1.1 Quality Control microballoons
(1) the preparation of the BGG of biotin labeling
With 0.1 M NaCNBH3BGG(is purchased from Pel-Freez Biological) it is configured to l0 mg/mL solution, it uses
DMSO(dimethylformamide) configuration Biotin-X-X-NHS(N- HOSu NHS modified biological element, manufacturer: SIGMA,
Production number: B3295) solution is to 16.2 mg/mL, according to the amount general of 1 mg BBG albumen addition, 5.4 μ, 1 Biotin-X-X-NHS
Biotin-X-X-NHS liquid is added in BGG solution, is uniformly mixed and is stood overnight at 4 DEG C.It is removed using dialysis free
Unreacted biotin, dialyzate are biotinylated protein elution buffer (0.1 M Tris, 0.3 M Nacl, 0.005 M
EDTA-Na-2H20, pH8.0).Dialysis finishes, and BCA method measures protein concentration.
(2) aldehyde group modified fluorescent microsphere is coated with using above-mentioned labelled protein
The above-mentioned (1) middle dialysis of Tween-20 solution, 2 mg that 6 μ l 20% are added in the fluorescent microsphere aldehyde group modified to 10mL obtains
Biotinylated protein and 16 μ l NaCNBH3, (25 mg/mL, the MES buffer of 0.05 M pH6.0, now match
It is current), adding 0.1 M pH6.0 MES to total volume is 400 μ l, and 37 DEG C are protected from light 48 h of incubation.The Gly solution of 40 μ l is added
(75 mg/mL, the MES buffer of 0.05 M pH6.0), 37 DEG C are protected from light 2 h of revolving reaction.Add 250 μ l N, 0- bis- three
First silicon substrate acetamide (200 mg/mL, the MES buffer of 0.05 M pH6.0) solution.37 DEG C are protected from light 16 h of revolving reaction.
4 DEG C of 13000 rpm is centrifuged 30 minutes.Supernatant is abandoned, is washed again twice with the MES buffer of 1 mL pH6.0.With 1 mL, 0.05 M
HEPES buffer solution (the 0.05 M HEPES, 0.3 M Nacl, 0.025 M EDTA-Na-2H of pH8.020,1.6% N, 0-is bis-
Trimethylsilyl acetamide (BSA), 0.1% Dextran, 0.1%Tween-20,0.3745% Triton X- 405,0.0l% celebrating are big
Mycin, 0.05 % Proclin) suspend (its final concentration of l0 mg/mL).
(3) Quality Control microballoon working solution is prepared
According to need of work, (2) respective concentration will be diluted to by middle suspension using the HEPES buffer solution of 0.05M pH 8.0, packing is protected
It deposits.
The preparation of 1.2 detection microballoons
Be added in the fluorescent microsphere aldehyde group modified to 10 mg Tween-20 solution, the 2 mg progesterone monoclonal antibodies of 6 μ l 20% with
And 16 μ l NaCNBH3(25 mg/mL, the MES buffer of 0.05 M pH6.0 are ready-to-use), adds 0.1 M
PH6.0 MES is 400 μ l to total volume, and 37 DEG C are protected from light 48 h of incubation.Gly solution (75 mg/mL, 0.05 M of 40 μ l are added
The MES buffer of pH6.0), 37 DEG C are protected from light 2 h of revolving reaction.Add 250 μ l BSA(200 mg/mL, 0.05 M pH6.0
MES buffer) solution.37 DEG C are protected from light 16 h of revolving reaction.4 DEG C of 13000 rpm is centrifuged 30 minutes.Supernatant is abandoned, with 1
The MES buffer of mL pH6.0 is washed twice again.With the HEPES buffer solution of 1 mL, 0.05 M pH8.0 (0.05 M HEPES, 0.3
M NaC1,0.25 M EDTA-Na-2H20,1.6% BSA, 0.1% Dextran, 0.1% Tween-20,0.3745% Triton
X-405,0.01% gentamicin, 0.05% Proclin) suspend (its final concentration of 10 mg/mL).
The configuration of 1.3 microspheres solutions
With release buffer (contain 20% sucrose, 5% trehalose, 0.5% BSA, 0.2% gentamicin) by Quality Control microballoon and detection
Microballoon is configured to time-resolved fluorescence microballoon mixed liquor, and final concentration of 0.2 mg/mL of Quality Control microballoon detects the final concentration of l of microballoon
mg/mL。
The preparation of 1.4 detection lines (T line) solution
Progesterone antigenic compound is diluted to 0.5 mg/mL with 0.01 M PB solution.
The preparation of 1.5 nature controlling lines (C line) solution
Streptavidin is diluted to 0.5 mg/mL with 0.01 M PB solution.
2. the preparation of test strips
2.1 blank kilocalories are pasted
According to the film combination of attached drawing 1, nitrocellulose filter 3 is located at centre, and Fusion5 film 2 is overlapped in respectively with blotting paper 4
Nitrocellulose filter left and right ends are pasted on the plastic bottom board with gum.
2.2 spray films
T is sprayed in the T line 7 in Fig. 1 respectively, 8 position of C line, and C line solution, C, it is 0.8 μ l/cm that T line, which sprays film liquid amount, 6 in Fig. 1
It sets and sprays microspheres solution, it is 4 μ l/cm that microspheres solution, which sprays film amount,.
2.3 drying
By step (2) in sprayed the progesterone of reagent and be stuck in constant temperature oven 37 DEG C greatly and dry 16 hours.
2.4 slitting
The progesterone kilocalorie of drying is cut into the paper slip of 4 mm width to get progesterone test strips are arrived.
3. detecting instrument principle and application
3.1 measuring instrument principles: condenser lens is located at exciting light into 90 degree of directions, generates the sample in sample detection pond
Fluorescence signal line focus lens focus, then photomultiplier tube is reached after grating beam splitting, photomultiplier tube converts light signals into
Electric signal is finally sent to A/D converter and is handled.The laser light source uses diode pumped solid state laser, output
Power is 20 mW, and launch wavelength is 375 ± 5 nm.Fiber power of the exciting light after conducting fiber coupling is 5 mW or more.
Ultraviolet quartz glass is selected in sample detection pond.Grating is set as that the light for obtaining that wavelength is 440 ± 5nm can be divided.
3.1 detector applications: the extract after pretreatment of the edible animal tissue containing progesterone is packed into sample detection
Chi Zhong, laser light source issue the laser that wavelength is 375 ± 5nm, conduct after conducting fiber coupling to sample detection pond, and pass through
Sample to be tested, the sample in sample detection pond, which absorbs, generates fluorescence after exciting light, the fluorescence signal line focus lens focus of generation,
Again through grating beam splitting, the light that wavelength is 440 ± 5 nm is obtained, photomultiplier tube is reached, photomultiplier tube converts light signals into
Electric signal, while input a/d converter after electric signal is amplified finally export and give reading plotter processing, be by reading plotter
Result can directly be read.
4. the quantitative detection of test strips
4.1 draw standard curve
Be added in the progesterone test strips sample application zone prepared various concentration progesterone antigen standard (take five it is different dense
Degree, respectively 0,10,30,90,270ng/mL, each concentration sets 5 repetitions), sample-loading buffer is added dropwise, and (PBS contains 1.6%
BSA, 0.1% Tween-20, preservative), after film layer is analysed 10 minutes, instrument reading C, T line signal,
Experimental result and analysis are shown in Table 1:
1 progesterone standard items testing result of table
Standard curve is drawn with antigen standard concentration and the signal averaging of measurement, using four parameter fitting modes, standard is bent
Line number evidence is shown in Table 2.
2 progesterone quantitative measurement standard curve data of table
| SPE standard items (ng/mL) | 0 | 10 | 30 | 90 | 270 |
| Reading | 18.918 | 12.48 | 8.536 | 3.706 | 1.44 |
4.2 sample detection
Sample to be tested is sequentially added in the sample application zone of progesterone test strips, sample-loading buffer is added dropwise, after film layer is analysed 10 minutes, instrument
Read C, T line signal.According to step (1) in standard curve calculate sample to be tested in progesterone antigen concentration.
5. test strips performance test
10 times crowdes of internal difference CV of 2 concentration samples are measured, experimental result is shown in Table 3, the experimental results showed that, batch interior precision of test strips
Degree is respectively less than 11%.
3 test strips of table batch internal difference
0-500 ng/mL can reach using progesterone quantitative testing test paper detection range prepared by the method, sensitivity is 10
For ng/mL hereinafter, withinrun precision is up to 11% or so, betweenrun precision can detect tissue, whole blood, serum, blood up to 15% simultaneously
Sample, 250 mg/dL hemoglobins, 500 mg/dL triglycerides are starched, 10 mg/dL bilirubin without influence, far surpass this detection
Cross most of immunochromatography quick diagnosis products in the market.
Claims (10)
1. progesterone rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper, it is characterised in that: including Fusion5 film, nitre
Acid cellulose film and blotting paper three parts, nitrocellulose filter are located at centre, and Fusion5 film and blotting paper are overlapped in nitre respectively
There are sample application zone and microballoon area in acid cellulose film left and right ends on Fusion5 film, and microballoon area is loaded with the progesterone monoclonal antibody time
Resolved fluorometric microballoon；There are detection line and nature controlling line on nitrocellulose filter, progesterone antigen is coated in detection line, is coated on nature controlling line
Anti-rabbit antibody.
2. test strips as described in claim 1, it is characterised in that: the time-resolved fluorescence microballoon includes detection microballoon and matter
Microballoon is controlled, detection microballoon is the fluorescent microsphere that pan coating has progesterone monoclonal antibody, and Quality Control microballoon is that pan coating has rabbit-anti to mark egg
White fluorescent microsphere.
3. test strips as claimed in claim 1 or 2, it is characterised in that: the particle size range of the time-resolved fluorescence microballoon is
100 - 1000 nm 。
4. test strips as described in claim 1, it is characterised in that: being coated with progesterone detection in the detection line with antigen is progesterone
The conjugates formed with carrier mass；Two anti-rabbit antibody are coated in detection line.
5. the preparation method of test strips as described in claim 1, preparation step are as follows:
(1) prepared by Quality Control microballoon:
1. using biotinylated protein；
2. being coated with aldehyde group modified fluorescent microsphere using above-mentioned labelled protein；
(2) detects microballoon preparation: aldehyde group modified fluorescent microsphere is coated with using the monoclonal antibody of progesterone；
(3) blank kilocalorie is pasted: nitrocellulose filter being pasted among plastic bottom board, Fusion5 film is taken respectively with blotting paper
It is connected to nitrocellulose filter left and right ends；
(4) sprays film: the fluorescent microsphere of the fluorescent microsphere of rabbit-anti labelled protein and progesterone monoclonal antibody is dilute using release buffer mixing
A certain concentration is released, the microballoon area of Fusion5 film is sprayed onto；After progesterone antigen and the dilution of anti-rabbit antibody, it is sprayed onto cellulose nitrate respectively
The T line and C line position of plain film；
(5) dry and slitting.
6. preparation method as claimed in claim 5, it is characterised in that: (4) middle release buffer group becomes 10-15% sugarcanes to step
Sugar, 3-10% trehaloses, 0. 5-1% N, the bis- trimethylsilyl acetamides (BSA) of O-, 0.1-0.5% gentamicin.
7. preparation method as claimed in claim 5, it is characterised in that: step detects microballoon final concentration of 0.5-after (4) diluting
3mg/mL, 0.05-0.3 mg/mL of Quality Control microballoon final concentration；0.5-3mg/mL of T line antigen final concentration, C line antigen final concentration
0. 5 - 3 mg/mL；It is 0.5-1.5 μ 1/cm that C, T line, which spray film liquid amount, and it is 2-10 μ l/cm that microballoon, which sprays film amount,.
8. preparation method as claimed in claim 5, it is characterised in that: (5) middle drying condition is 37 DEG C of drying 12-16 to step
Hour.
9. the progesterone rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper as described in claim 5 claim,
Be characterized in that: test strips bottom is equipped with plastic bottom board.
10. the application of test strips as described in claim 1, it is characterised in that: the test strips are used for using competition law mode
The quantitative detection of immunochromatography.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711150978.6A CN109813889A (en) | 2017-11-18 | 2017-11-18 | The time-resolved fluoroimmunoassay for detecting progesterone chromatographs quantitative testing test paper item |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326976A (en) * | 2020-11-04 | 2021-02-05 | 瑞莱生物科技江苏有限公司 | Fluorescence quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin |
| CN112326976B (en) * | 2020-11-04 | 2024-04-26 | 瑞莱生物科技江苏有限公司 | Fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotrophin |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2402147A1 (en) * | 2000-03-15 | 2001-09-20 | Merck & Co., Inc. | Melanin concentrating hormone receptor chimeric and fusion proteins |
| US20100069298A1 (en) * | 2008-09-16 | 2010-03-18 | Robert Penny | Methods for Detecting Overexpression of SPARC and Therapeutic and Diagnostic Methods Relating to Same |
| CN102192983A (en) * | 2011-05-19 | 2011-09-21 | 博阳生物科技（上海）有限公司 | Time-resolved immunoflourometric chromatographic test strip for quantitative detection as well as preparation method and application thereof |
| CN103792357A (en) * | 2012-11-05 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip |
| CN104655846A (en) * | 2013-11-20 | 2015-05-27 | 镇江先创生物科技有限公司 | Enzyme linked immunosorbent assay kit for detecting progesterone and detection method thereof |
| CN105572345A (en) * | 2014-10-16 | 2016-05-11 | 镇江亿特生物科技发展有限公司 | Fluorescence assay method for detecting neomycin in chicken meat tissue |
| CN106596949A (en) * | 2015-10-15 | 2017-04-26 | 南京亿特生物科技有限公司 | Time resolved fluorescence immunoassay kit for detecting butachlor |
| CN106706931A (en) * | 2016-12-15 | 2017-05-24 | 威海纽普生物技术有限公司 | Progesterone measurement kit and preparation method |
| CN107110860A (en) * | 2014-08-30 | 2017-08-29 | 新加坡科技研究局 | The test-strips determined for paper substrate |
-
2017
- 2017-11-18 CN CN201711150978.6A patent/CN109813889A/en not_active Withdrawn
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2402147A1 (en) * | 2000-03-15 | 2001-09-20 | Merck & Co., Inc. | Melanin concentrating hormone receptor chimeric and fusion proteins |
| US20100069298A1 (en) * | 2008-09-16 | 2010-03-18 | Robert Penny | Methods for Detecting Overexpression of SPARC and Therapeutic and Diagnostic Methods Relating to Same |
| CN102192983A (en) * | 2011-05-19 | 2011-09-21 | 博阳生物科技（上海）有限公司 | Time-resolved immunoflourometric chromatographic test strip for quantitative detection as well as preparation method and application thereof |
| CN103792357A (en) * | 2012-11-05 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip |
| CN104655846A (en) * | 2013-11-20 | 2015-05-27 | 镇江先创生物科技有限公司 | Enzyme linked immunosorbent assay kit for detecting progesterone and detection method thereof |
| CN107110860A (en) * | 2014-08-30 | 2017-08-29 | 新加坡科技研究局 | The test-strips determined for paper substrate |
| CN105572345A (en) * | 2014-10-16 | 2016-05-11 | 镇江亿特生物科技发展有限公司 | Fluorescence assay method for detecting neomycin in chicken meat tissue |
| CN106596949A (en) * | 2015-10-15 | 2017-04-26 | 南京亿特生物科技有限公司 | Time resolved fluorescence immunoassay kit for detecting butachlor |
| CN106706931A (en) * | 2016-12-15 | 2017-05-24 | 威海纽普生物技术有限公司 | Progesterone measurement kit and preparation method |
Non-Patent Citations (3)
| Title |
|---|
| GEERTRUIDA A 等: ""Development of a competitive lateral flow immunoassay for progesterone: influence of coating conjugates and buffer components"", 《ANALYTICAL AND BIOANALYTICAL CHEMISTRY》 * |
| 冯晓群 等: "奶牛乳汁孕酮免疫检测方法研究进展"", 《动物医学进展》 * |
| 高平 等: ""新型固相竞争性时间分辨荧光免疫检测血清孕酮方法的建立"", 《中国免疫学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326976A (en) * | 2020-11-04 | 2021-02-05 | 瑞莱生物科技江苏有限公司 | Fluorescence quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin |
| CN112326976B (en) * | 2020-11-04 | 2024-04-26 | 瑞莱生物科技江苏有限公司 | Fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotrophin |
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