CN109813844A - A method of identification and evaluation blueberry salt tolerance - Google Patents
A method of identification and evaluation blueberry salt tolerance Download PDFInfo
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- CN109813844A CN109813844A CN201910033997.3A CN201910033997A CN109813844A CN 109813844 A CN109813844 A CN 109813844A CN 201910033997 A CN201910033997 A CN 201910033997A CN 109813844 A CN109813844 A CN 109813844A
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Abstract
The present invention relates to agricultural technology field, the method for specifically a kind of identification and evaluation blueberry salt tolerance.It the described method comprises the following steps: (1) using 2 years raw blueberry Potted orchards as material, growth and Determination of Physiological And Biochemical Indices are carried out after being handled 40 days with various concentration NaCl；(2) membership function value and average value of blueberry salt tolerant index of correlation are calculated using subordinate functional analysis method；(3) compare the average membership function value of different cultivars blueberry salt-tolerance index.Average membership function value is bigger, and blueberry kind salt tolerance is stronger.The method of the present invention establishes the blueberry salt tolerance overall evaluation system of multi objective, and evaluation result is accurate and reliable, the identification and evaluation suitable for different cultivars blueberry salt tolerance.
Description
Technical field
The present invention relates to agricultural technology field, the method for specifically a kind of identification and evaluation blueberry salt tolerance.
Background technique
Currently, Chinese about 36,000,000 hm of the salinized soil gross area2, the 4.48% of land area can be utilized by accounting for the whole nation, intertillage
Ground medium salting area reaches 920.9hm2, Zhan Quanguo cultivated area 6.62%.And saline alkali land area in year by year it is widened become
Gesture.With industrial development, cultivated area reduce, the saline-alkali soil land reserved resources important as one and be taken seriously.Thus
Economic plants Mechanisms of Salt Resistance, breeding and exploitation salt tolerant economic plants kind are explored, is rationally and effectively to be increased using salinization land
Add economic benefit, the important channel of developing rural economy.
Blueberry is Ericaceae (Ericaceae) genus vaccinium (Vaccinium spp.) plant, the anthocyanin tool in fruit
There are anti-oxidant, removing free radical, anticancer and improve the bioactivity such as eyesight, there is huge healthcare function.In recent years, the whole world
Blueberry production scale is increasing, and important function is played in developing rural economy as novel economizer tree kind.Research shows that
Height has certain salt tolerance from blueberry, but different blueberry kind salt tolerance difference degrees are unclear, need to establish science
Identification and appraisement system.Currently, the morphological indexs such as upgrowth situation had usually both been considered in plant salt endurance evaluation, it is further contemplated that physiology
Biochemical indicator.This method is relatively scientific.If Zhu Yan virtue and Li Yumei are respectively to different cultivars apple and Portugal under salt stress
Grape growth indexes and physical signs carry out Subordinate Function and are analyzed, determine its salt-resistant type size (Zhu Yanfang, Wang Yanxiu,
Chen Baihong, the physiological responses for waiting tetra- kinds of apple rootstock seedling of to coerce NaCl, root growth and Evaluation of Salt Tolerance [J] Gansu agriculture
Industry college journal, 2017,52 (2): 48-53；Physiology of the tetra- raspberry cultivars of Li Yumei, Jiang Yuntian to mixed salt stress
Response and its north Evaluation of Salt Tolerance [J] gardening, 2018,15:18-26).Establish scientific and effective blueberry Salt-Tolerance Identification and
Appraisement system provides foundation for the selection and determining reasonable cultivation management measure of salt-soda soil region salt tolerant blueberry kind.
Summary of the invention
The object of the present invention is to provide a kind of methods of identification and evaluation blueberry salt tolerance, with 2 years raw different cultivars blueberries
Blueberry salt tolerance is carried out using Subordinate Function to growth and physiological index determining under various concentration salt stress for expert evidence
Overall merit, the purpose of the present invention is what is be achieved through the following technical solutions:
A method of identification and evaluation blueberry salt tolerance the described method comprises the following steps:
(1) by the blueberry material that need to be identified use respectively various concentration NaCl handle after, measure it is relevant to salt tolerant grow and
Physiological and biochemical index, the growth relevant to salt tolerance and physiological and biochemical index are as follows: salt gypsum rock, soluble sugar content, dried meat
Histidine content, mda content, relative conductivity, chlorophyll content；
(2) growth obtained step (1) and physiological and biochemical index carry out membership function value calculating, then each kind
Membership function value of each index under various concentration salt stress is cumulative to average, and obtains being subordinate to letter under the kind index
Numerical value, then the membership function value of each index of each kind is added up and is averaged, as the salt tolerance of the kind blueberry is subordinate to
Membership fuction average value；The calculation formula of the membership function value are as follows:
Membership function value calculation formula Uij=(Xij-Xjmin)/(Xjmax-Xjmin)
Anti- membership function value calculation formula Uij=1- (Xij-Xjmin)/(Xjmax-Xjmin)
Wherein, i indicates some blueberry kind；J indicates some salt-tolerance index, UijIndicate that the salt tolerant of i kind j index is subordinate to letter
Numerical value；XijIndicate i kind j index determining value；XjminIndicate the minimum value of all kind j indexs, XjmaxIndicate that all kind j refer to
Target maximum value；
(3) the salt tolerance membership function average value size for the different cultivars blueberry that comparison step (2) obtains, evaluates its salt tolerant
Property it is strong and weak, membership function average value is bigger, and salt tolerance is stronger, and membership function average value is smaller, and salt tolerance is weaker.
A kind of method of above-mentioned identification and evaluation blueberry salt tolerance, the blueberry material are that 2 years raw potting high clump blueberries are " double
Enlightening ", " Ao Ni'er " and " Lan Feng " cuttage seeding.
A kind of method of above-mentioned identification and evaluation blueberry salt tolerance, in step (1) NaCl concentration used be 0,100,200,
300mmol·L-1, handle 40 days.
A kind of method of above-mentioned identification and evaluation blueberry salt tolerance, salt gypsum rock=∑ (salt damage series described in step (1)
× corresponding salt damage grade plant number)/(total strain number × salt damage highest series) × 100%；
The soluble sugar content measurement uses anthrone method；The proline content measurement uses acid ninhydrine colorimetric
Method；The mda content measurement uses thiobarbituricacidα- method；The blade relative conductivity measurement uses conductivity gauge method；Institute
State measuring chlorophyll content acetone extraction.
A kind of method of above-mentioned identification and evaluation blueberry salt tolerance, the salt damage series is according to salt stress extent of injury weight
It is divided into 5 ranks and gives every seedlings score and comments, 0 grade: without salt stress hazard symptoms, 1 grade: small part blade tip, leaf margin and leaf
Arteries and veins turns yellow, and blade tip, the leaf margin of 2 grades: about 1/2 blades are shrivelled, and 3 grades: most of blade has a shrivelled phenomenon of blade tip, leaf margin, and 4 grades: branch
Withered, leaf is fallen until dead.
Compared with prior art, the invention has the following beneficial effects: the present invention provides a kind of blueberry Salt-Tolerance Identifications and evaluation
Method, this method by Subordinate function value method analyze blueberry it is relevant to salt tolerant growth and physiological and biochemical index and blueberry it is resistance to
The membership of salt establishes the salt tolerant integrated evaluating method of blueberry multi objective.The integrated evaluating method can accurately objectively
Identify blueberry kind salt tolerances different from evaluation.
Specific embodiment
The present invention is described in more detail below in conjunction with specific embodiment.
Embodiment 1
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
The method of the blueberry Salt-Tolerance Identification and evaluation of the present embodiment, comprising the following steps:
(1) test carries out in the greenhouse of University Of Dalian's modern agriculture proving ground in the 14-8 month 23 in July, 2015.Test
Material is life in 2 years " double enlightening ", " Ao Ni'er " and " Lan Feng " potting cuttage seeding.It is equipped with 0,100,200,300mmolL- altogether1NaCl
4 processing, 3 repetitions of each processing, each 3 basin of repetition are that is, each to handle totally 9 basin.Every 5 days salting liquids with respective concentration
It pours, every basin pours 0.5L.Plastic pallet is put at flowerpot bottom, if there is solution outflow, is rewound in basin.Deionization is used before pouring every time
Water sufficiently elutes matrix, to ensure the accuracy of experimental design.Stress treatment 40 days altogether.3 kind indigo plants are measured after the test
Certain kind of berries blade and the significant relevant growth of salt tolerance and physiological and biochemical index, the index are as follows: salt gypsum rock, soluble sugar content,
Proline content, mda content, relative conductivity and chlorophyll content.It is divided into 5 grades according to salt stress extent of injury weight
Not and give the evaluation of every seedlings score, 0 grade: without salt stress hazard symptoms；1 grade: small part blade tip, leaf margin and vein turn yellow；2
Grade: about 1/2 blade tip, leaf margin are shrivelled；3 grades: most of blade has blade tip, leaf margin shrivelled and fallen leaves phenomenon；4 grades: branch is withered, Ye Luo
Until dead.Salt gypsum rock calculation formula are as follows:
Salt gypsum rock=∑ (salt damage series × corresponding salt damage grade plant number)/(total strain number × salt damage highest series) ×
100%
Soluble sugar content is measured using anthrone method；Using acid ninhydrine colorimetric method for determining proline content；Using sulphur
MDA content is measured for barbital acid system；Branch relative conductivity is measured using conductivity gauge method；It is green using acetone extraction measurement leaf
Cellulose content.Measurement result is shown in Table 1.
Growth and physiological and biochemical index after the different blueberry kind various concentration salt stress of table 1
(2) it carries out the growth of step (1) acquisition and physiological and biochemical index to carry out membership function value calculating, then each
Membership function value of each index of kind under various concentration salt stress is cumulative to average, and obtains the person in servitude under the kind index
Membership fuction value, then average the membership function value of each index of each kind is cumulative, the as salt tolerant of the kind blueberry
Property membership function average value；The calculation formula of the membership function value are as follows:
Membership function value calculation formula Uij=(Xij-Xjmin)/(Xjmax-Xjmin)
Anti- membership function value calculation formula Uij=1- (Xij-Xjmin)/(Xjmax-Xjmin)
Wherein, i indicates some blueberry kind；J indicates some salt-tolerance index, UijIndicate that the salt tolerant of i kind j index is subordinate to letter
Numerical value；XijIndicate i kind j index determining value；XjminIndicate the minimum value of all kind j indexs, XjmaxIndicate that all kind j refer to
Target maximum value；Different blueberry kind membership function values and membership function average value are shown in Table 2.
(3) the salt tolerance membership function average value size for the different cultivars blueberry that comparison step (2) obtains, evaluates its salt tolerant
Property it is strong and weak, average value is bigger, and salt tolerance is stronger, conversely, salt tolerance is weaker.The result shows that 3 blueberry kind salt tolerances by by force to
Weak sequence is double enlightening > > Lan Feng > Ao Ni'ers.
The membership function value and average value of 2 blueberry salt tolerance index of table
| Kind | Salt gypsum rock | Soluble sugar | Proline | Malonaldehyde | Conductivity | Chlorophyll | Mean value | Sequence |
| Double enlightening | 0.652 | 0.666 | 0.597 | 0.623 | 0.617 | 0.563 | 0.620 | 1 |
| Ao Ni'er | 0.505 | 0.145 | 0.547 | 0.439 | 0.429 | 0.34 | 0.401 | 3 |
| Lan Feng | 0.609 | 0.242 | 0.631 | 0.588 | 0.521 | 0.468 | 0.510 | 2 |
Claims (5)
1. a kind of method of identification and evaluation blueberry salt tolerance, which is characterized in that the described method comprises the following steps:
(1) after using various concentration NaCl to handle respectively the blueberry material that need to be identified, growth relevant to salt tolerant and physiology are measured
Biochemical indicator, the growth relevant to salt tolerance and physiological and biochemical index are as follows: salt gypsum rock, soluble sugar content, proline
Content, mda content, relative conductivity, chlorophyll content；
(2) growth obtained step (1) and physiological and biochemical index carry out membership function value calculating, then that each kind is each
Membership function value of the index under various concentration salt stress is cumulative to average, and obtains the membership function under the kind index
Value, then the membership function value of each index of each kind is added up and is averaged, the salt tolerance of the as kind blueberry is subordinate to
Mean value functions；The calculation formula of the membership function value are as follows:
Membership function value calculation formula Uij=(Xij-Xjmin)/(Xjmax-Xjmin)
Anti- membership function value calculation formula Uij=1- (Xij-Xjmin)/(Xjmax-Xjmin)
Wherein, i indicates some blueberry kind；J indicates some salt-tolerance index, UijIndicate the salt tolerant membership function of i kind j index
Value；XijIndicate i kind j index determining value；XjminIndicate the minimum value of all kind j indexs, XjmaxIndicate all kind j indexs
Maximum value；
(3) the salt tolerance membership function average value size for the different cultivars blueberry that comparison step (2) obtains, it is strong to evaluate its salt tolerance
Weak, membership function average value is bigger, and salt tolerance is stronger, and membership function average value is smaller, and salt tolerance is weaker.
2. a kind of method of identification and evaluation blueberry salt tolerance as described in claim 1, which is characterized in that the blueberry material
For 2 years raw potting high clump blueberries " double enlightening ", " Ao Ni'er " and " Lan Feng " cuttage seedings.
3. a kind of method of identification and evaluation blueberry salt tolerance as described in claim 1, which is characterized in that institute in step (1)
It is 0,100,200,300mmolL with NaCl concentration-1, handle 40 days.
4. a kind of method of identification and evaluation blueberry salt tolerance as described in claim 1, which is characterized in that institute in step (1)
State salt gypsum rock=∑ (salt damage series × corresponding salt damage grade plant number)/(total strain number × salt damage highest series) × 100%；
The soluble sugar content measurement uses anthrone method；The proline content measurement uses acid ninhydrine colorimetric method；Institute
Mda content measurement is stated using thiobarbituricacidα- method；The blade relative conductivity measurement uses conductivity gauge method；The leaf
Chlorophyll contents measurement acetone extraction.
5. a kind of method of identification and evaluation blueberry salt tolerance as claimed in claim 4, which is characterized in that the salt damage series
It is divided into 5 ranks according to salt stress extent of injury weight and gives every seedlings score and comments, 0 grade: without salt stress hazard symptoms, 1
Grade: small part blade tip, leaf margin and vein turn yellow, and blade tip, the leaf margin of 2 grades: about 1/2 blades are shrivelled, and 3 grades: most of blade has leaf
Point, the shrivelled phenomenon of leaf margin, 4 grades: branch is withered, leaf is fallen until dead.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110476635A (en) * | 2019-09-03 | 2019-11-22 | 南通大学 | The sticking device and its screening technique of forest screening salt-enduring cultivars difficult to take root |
| CN111855920A (en) * | 2020-08-05 | 2020-10-30 | 中国农业科学院棉花研究所 | Method for identifying alkali stress degree of cotton |
| CN111855751A (en) * | 2020-08-14 | 2020-10-30 | 南通大学 | Method for rapidly evaluating salt tolerance of willow variety based on resistance value |
| CN114009326A (en) * | 2021-11-05 | 2022-02-08 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of in-vitro water culture of plant |
| CN116895327A (en) * | 2023-07-12 | 2023-10-17 | 中国热带农业科学院热带作物品种资源研究所 | Comprehensive evaluation method for zoysia japonica germplasm salt tolerance |
| CN117054609A (en) * | 2023-08-14 | 2023-11-14 | 中国热带农业科学院热带作物品种资源研究所 | Shadow tolerance evaluation method for blunt leaf grass germplasm resources |
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| CN107966434A (en) * | 2017-07-03 | 2018-04-27 | 扬州大学 | A kind of method of salt tolerance of eggplant Rapid identification |
Non-Patent Citations (2)
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476635A (en) * | 2019-09-03 | 2019-11-22 | 南通大学 | The sticking device and its screening technique of forest screening salt-enduring cultivars difficult to take root |
| CN111855920A (en) * | 2020-08-05 | 2020-10-30 | 中国农业科学院棉花研究所 | Method for identifying alkali stress degree of cotton |
| CN111855751A (en) * | 2020-08-14 | 2020-10-30 | 南通大学 | Method for rapidly evaluating salt tolerance of willow variety based on resistance value |
| CN111855751B (en) * | 2020-08-14 | 2021-02-19 | 南通大学 | Method for rapidly evaluating salt tolerance of willow variety based on resistance value |
| CN114009326A (en) * | 2021-11-05 | 2022-02-08 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of in-vitro water culture of plant |
| CN116895327A (en) * | 2023-07-12 | 2023-10-17 | 中国热带农业科学院热带作物品种资源研究所 | Comprehensive evaluation method for zoysia japonica germplasm salt tolerance |
| CN117054609A (en) * | 2023-08-14 | 2023-11-14 | 中国热带农业科学院热带作物品种资源研究所 | Shadow tolerance evaluation method for blunt leaf grass germplasm resources |
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