CN109810152A - A kind of glycosides displayed biology extraction method - Google Patents
A kind of glycosides displayed biology extraction method Download PDFInfo
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- CN109810152A CN109810152A CN201711165090.XA CN201711165090A CN109810152A CN 109810152 A CN109810152 A CN 109810152A CN 201711165090 A CN201711165090 A CN 201711165090A CN 109810152 A CN109810152 A CN 109810152A
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- glycosides displayed
- extraction method
- ginkgo leaf
- glycosides
- displayed
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Abstract
The invention discloses a kind of glycosides displayed biology extraction methods, using ginkgo leaf as raw material, include the following steps: that (1) crushes ginkgo leaf；(2) complex enzyme is added, is digested 36 hours, 35 DEG C of hydrolysis temperature；(3) obtained enzymolysis liquid filtering removal filter residue is extracted, obtains filtrate, filtrate is glycosides displayed crude product；(4) crystallisation obtains glycosides displayed sterling.Advantages of the present invention: being using biological extraction process method simplicity, and recovery rate is high, and recovery rate reaches 80% or more.
Description
Technical field
The invention belongs to field of biotechnology more particularly to a kind of glycosides displayed biology extraction methods.
Background technique
Biotechnology (biotechnology) refers to people based on modern life science, in conjunction with other basic science
The principles of science using advanced scientific and technological means, according to preparatory design improvement organism or processing biological raw material, be people
Class produces required product or reaches certain purpose.
Biotechnology is that people process raw material of substance using microorganism, animal and plant body, is come with providing product for society
The technology that can be serviced.It mainly includes fermentation technique and modern biotechnology.Therefore, biotechnology is one emerging, comprehensive
The subject of property.
Modern biotechnology comprehensive gene engineering, molecular biology, biochemistry, science of heredity, cell biology, embryo
, immunology, microbiology, chemical biology, organic chemistry, inorganic chemistry, physical chemistry, physics, informatics and calculating
The multidisciplinary technology such as machine science, can be used for studying the movable rule of life and provide product is community service etc..
Biofermentation purification be exactly utilize microorganism specific trait and function, by modernization project technology and equipment come
Microorganism is directly used in the technical system that industrialized production obtains purpose product by production utility.Including fermenting and purifying
Two processes.Purification is the process for separating required product from fermentation liquid, is also referred to as post-processed.It mainly include cell
It is broken, separation, mash conveying, filtering and impurity removing, ion exchange electrodialysis, reverse osmosis, ultrafiltration, gel filtration (chromatography), precipitating point
From solvent extraction evaporates, and crystallizes, distillation, the processes such as dry packing and unit operation.
The chemical component of ginkgo leaf is extremely complex, and type is more, and there are many ingredients to have physiological activity.According to medicine,
The disciplinary studies such as chemistry and molecular biology show that ginkgo leaf main chemical compositions have: flavonoids, terpene, ginnol, thick grass
Acid, sitosterol, in addition, there are also alkaloid, polyisoprene, a variety of amino acid, minerals, polysaccharide, procyanidine, tannins, silver
Apricot phenolic acid, wax, chlorophyll etc..Glycosides displayed (flavonoid glycosides) is also known as flavone class, is to be widely present in plant
A kind of natural pigment on boundary.Content is very high in ginkgo leaf, but there is presently no specially biology extracts glycosides displayed from ginkgo leaf
Method.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of glycosides displayed biology extraction methods, can be effective
Extract glycosides displayed.
The present invention is realized by the following scheme:
A kind of glycosides displayed biology extraction method includes the following steps: using ginkgo leaf as raw material
(1) ginkgo leaf is crushed；
(2) complex enzyme is added, is digested 36 hours, 35 DEG C of hydrolysis temperature；
(3) obtained enzymolysis liquid filtering removal filter residue is extracted, obtains filtrate, filtrate is glycosides displayed crude product；
(4) crystallisation obtains glycosides displayed sterling.
Further, ginkgo leaf is crushed 100 mesh by step (1).
Further, step (2) complex enzyme is the mixture of lignin-degrading enzymes, cellulase and pectase.
Further, the mass ratio of the lignin-degrading enzymes, cellulase and pectase is 2:1:1.
Further, step (3) filtering is filtered out using film, and filtering is at least three times.
Lignin is the biopolymer that the content being present in higher plant cell wall is only second to cellulose, is accounted for about woody
The 25% of plant plays a part of reinforcing plant tissue.In plant tissue, lignin, cellulose and hemicellulose are with covalent
The engagement of key-shaped formula, cellulosic molecule is embedded in and wherein forms firm natural barrier, general microorganism is made to hardly enter it
Can middle decomposition of cellulose, therefore, the degradation of lignin be the key that efficiently use one of cellulose.In the carbon of nature
In circulation, the degradation and utilization of lignin are the speed limit links of most critical.
Cellulase (beta-1,4-glucan -4- glucan hydrolase) is the glucogenic one group of enzyme of degraded cellulose
General name, its not instead of monomeric enzyme play the multicomponent enzyme system of synergistic effect, are a kind of complex enzymes, mainly by circumscribed beta glucan
The composition such as enzyme, Endo-β-glucanase and beta-glucosidase, there are also the zytases of very high vigor.Act on cellulose and
The product being derived from cellulose.Microbial cellulase is in conversion insoluble fibrin at glucose and in juice
Cell wall is destroyed to have very important significance to improve fruit juice yield etc..
Advantages of the present invention: being using biological extraction process method simplicity, and recovery rate is high, and recovery rate reaches 80% or more.
Specific embodiment
It, below will be to embodiments of the present invention in order to keep features described above and advantage of the present invention more clear and be readily appreciated that
It is described in further detail.
Embodiment 1
A kind of glycosides displayed biology extraction method includes the following steps: using ginkgo leaf as raw material
(1) ginkgo leaf is crushed；
(2) complex enzyme is added, is digested 36 hours, 35 DEG C of hydrolysis temperature；
(3) obtained enzymolysis liquid filtering removal filter residue is extracted, obtains filtrate, filtrate is glycosides displayed crude product；
(4) crystallisation obtains glycosides displayed sterling.
Further, ginkgo leaf is crushed 100 mesh by step (1).
Further, step (2) complex enzyme is the mixture of lignin-degrading enzymes, cellulase and pectase.
Further, the mass ratio of the lignin-degrading enzymes, cellulase and pectase is 2:1:1.
Further, step (3) filtering is filtered out using film, and filtering is at least three times.
The preferred embodiment for stating only the invention is not intended to limit the invention creation, all to create in the present invention
Within the spirit and principle made, any modification, equivalent replacement, improvement and so on should be included in the protection of the invention
Within the scope of.
Claims (5)
1. a kind of glycosides displayed biology extraction method, using ginkgo leaf as raw material, characterized by the following steps:
(1) ginkgo leaf is crushed；
(2) complex enzyme is added, is digested 36 hours, 35 DEG C of hydrolysis temperature；
(3) obtained enzymolysis liquid filtering removal filter residue is extracted, obtains filtrate, filtrate is glycosides displayed crude product；
(4) crystallisation obtains glycosides displayed sterling.
2. a kind of glycosides displayed biology extraction method according to claim 1, which is characterized in that step (1) crushes ginkgo leaf
100 mesh.
3. a kind of glycosides displayed biology extraction method according to claim 1, which is characterized in that step (2) described complex enzyme is
The mixture of lignin-degrading enzymes, cellulase and pectase.
4. a kind of glycosides displayed biology extraction method according to claim 3, which is characterized in that the lignin-degrading enzymes, fibre
The mass ratio for tieing up plain enzyme and pectase is 2:1:1.
5. a kind of glycosides displayed biology extraction method according to claim 1, which is characterized in that step (3) filtering uses
Film filters out, and filtering is at least three times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711165090.XA CN109810152A (en) | 2017-11-21 | 2017-11-21 | A kind of glycosides displayed biology extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711165090.XA CN109810152A (en) | 2017-11-21 | 2017-11-21 | A kind of glycosides displayed biology extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109810152A true CN109810152A (en) | 2019-05-28 |
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| CN201711165090.XA Pending CN109810152A (en) | 2017-11-21 | 2017-11-21 | A kind of glycosides displayed biology extraction method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022166839A1 (en) * | 2021-02-05 | 2022-08-11 | 汤臣倍健股份有限公司 | Use of ginkgo leaf extract in preparation of drug for targeting senescent cells, inhibiting tumors or prolonging lifetime |
-
2017
- 2017-11-21 CN CN201711165090.XA patent/CN109810152A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022166839A1 (en) * | 2021-02-05 | 2022-08-11 | 汤臣倍健股份有限公司 | Use of ginkgo leaf extract in preparation of drug for targeting senescent cells, inhibiting tumors or prolonging lifetime |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190528 |
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| WD01 | Invention patent application deemed withdrawn after publication |