CN109806922A - A kind of the pipe lid and pipe lid adaptive device used in conjunction with of suitable puncture - Google Patents

A kind of the pipe lid and pipe lid adaptive device used in conjunction with of suitable puncture Download PDF

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Publication number
CN109806922A
CN109806922A CN201711161137.5A CN201711161137A CN109806922A CN 109806922 A CN109806922 A CN 109806922A CN 201711161137 A CN201711161137 A CN 201711161137A CN 109806922 A CN109806922 A CN 109806922A
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CN
China
Prior art keywords
pipe lid
pipe
lid
sealing structure
adaptive device
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711161137.5A
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Chinese (zh)
Inventor
白宇
苏世圣
王博
荆高山
郭永
王勇斗
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Xinyi Biotechnology Co Ltd
Tsinghua University
Original Assignee
Beijing Xinyi Biotechnology Co Ltd
Tsinghua University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Beijing Xinyi Biotechnology Co Ltd, Tsinghua University filed Critical Beijing Xinyi Biotechnology Co Ltd
Priority to CN201711161137.5A priority Critical patent/CN109806922A/en
Publication of CN109806922A publication Critical patent/CN109806922A/en
Pending legal-status Critical Current

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Abstract

The present invention provides the pipe lid and pipe lid adaptive device used in conjunction with of a kind of suitable puncture, and the pipe lid is integrally machined molding by rubber material;The pipe lid includes the upper sealing structure positioned at pipe lid external upper, inner wall sealing structure and outer wall sealing structure and thin-walled paracentesis area inside the pipe lid.The pipe lid adaptive device includes substrate, the puncture needle and circumferential seal set of setting on the substrate.It is convenient that the Guan Gai and the close fastening of pipe, Guan Gai and pipe open and close;Pipe lid is punctured using metal, plastic ware, sample it is convenient.

Description

A kind of the pipe lid and pipe lid adaptive device used in conjunction with of suitable puncture
Technical field
The present invention relates to rubber processings, and in particular to a kind of the pipe lid and pipe lid used in conjunction with of suitable puncture Adaptive device.
Background technique
Micro centrifugal pipe, also known as EP (eppendorf) pipe, match with micro centrifuge, for trace reagent Centrifugation and separation.EP pipe had become dedicated generation of the indispensable small size synthetic resin quality tubular container in laboratory and claimed today.Mesh Preceding common EP pipe has 5ml round bottom centrifuge tube, 2ml scale round bottom centrifuge tube, 1.5ml scale conical centrifuge tube, 0.5ml scale point Bottom centrifuge tube, 0.5ml thin-walled conical centrifuge tube, 0.2ml scale point bottom flat cover centrifuge tube and the centrifugation of 0.2ml scale point bottom spherical cap Pipe etc..
In general, managing matching used pipe lid function demand with EP includes: (1) pipe lid and the close fastening of EP pipe.EP Guan Zhongtong It is often equipped with biological reagent, needs to react (such as needing 95 DEG C of denaturation of experience in PCR reaction process) by high-temperature biological.Pyroreaction Under, it manages interior gas and expands.Therefore EP pipe pipe lid needs under heating state, and the tolerance bulbs of pressure are without leaking.(2) It is convenient that pipe lid is opened and closed with EP pipe.User opens pipe lid, and when extracting the biological reagent in EP pipe, required power is small, it is not easy to occur Biological reagent splash phenomena in EP pipe.(3) using metal, plastic ware or pipe lid adaptive device to EP pipe pipe Gai Jinhang Puncture, sample it is convenient.It is managed when user pierces through EP using metal, plastic ware (syringe, liquid-transfering gun) or pipe lid adaptive device Pipe lid extracts the biological reagent in EP pipe, and it is convenient to need to puncture, and air-tightness is good at metal, plastic ware and pipe lid puncture.
Managing matching used pipe lid material with EP at present is the rigid plastics as managing material itself with EP.Plastic conduit Lid can manage close fastening, tolerance high-temperature biological reaction (such as PCR reaction) with EP.Close button is covered tightly in order to reach EP pipe with EP pipe The purpose of conjunction, conventional EP pipe nozzle outer are a cyclic structures.But common stereoplasm tube lid the problem is that: (1) pipe lid with The folding of EP pipe is more difficult.User opens pipe lid, and required power is big, the biological reagent splash phenomena being easy to happen in EP pipe.(2) it uses Metal, plastic ware puncture EP pipe pipe lid, sample difficulty.Metal, plastic ware pierce through rigid plastics difficulty;Even if wearing It pierces successfully, air-tightness is bad at metal, plastic ware and pipe lid puncture.
Summary of the invention
For the deficiency of existing pipe lid, the present invention provides a kind of pipe lid, and the pipe lid is integrally formed by rubber material;It is described Pipe lid includes the upper sealing structure positioned at pipe lid external upper, and the inner wall sealing structure and outer wall inside the pipe lid are close Seal structure and thin-walled paracentesis area;It is located within region defined by the upper sealing structure with thin-walled paracentesis area upper surface, Thin-walled paracentesis area lower surface is located within region defined by the inner wall sealing structure.The thin-walled paracentesis area is covered in pipe The region for being suitable for puncturing being arranged, the pipe lid thickness in the region is smaller than peripheral region thickness under normal circumstances, in pipe Gai Yiti In molding, cover to form this thin-walled paracentesis area thinner than peripheral thickness in pipe using pipe lid processing mold, be suitable for into The puncture of the hand-manipulating of needle.Since the pipe lid is made of rubber material, rubber material refers to the strong flexible polymer with reversible deformation Material, at room temperature high resilience can generate larger deformation under the external force of very little, remove resilient after external force. After puncture needle punctures thin-walled paracentesis area, it can restore to the original state faster.
In a kind of mode, the pipe lid includes the circumferential closed structure outside pipe lid.
In one embodiment, the rubber material is silicon rubber.Certain rubber material is also possible to natural rubber, closes At natural rubber, styrene rubber, butadiene rubber, neoprene, butyl rubber, nitrile rubber, the third glue of second, chlorosulfonated polyethylene glue, Acrylic adhesive, polyurethane rubber, fluorubber, polysulfide rubber, chlorohydrin rubber, epichlorohydrin rubber and chlorinated polyethylene rubber etc..
In a kind of mode, the upper sealing structure is projecting annular structure and/or the institute of the pipe lid external upper Stating circumferential closed structure is the projecting annular structure around the pipe lid outer lower.
In a kind of mode, the inner wall sealing structure and outer wall sealing structure are carried out with inside pipe wall and pipe outer wall respectively The cyclic structure of cooperation.
In a kind of mode, the present invention provides a kind of pipe lid arrangement, and the pipe lid arrangement includes above-mentioned multiple pipe lids, described It is attached between multiple pipe lids by attachment device.
In a kind of mode, the circumferential closed structure of adjacent tubes lid is connected to each other integrally.
In one embodiment, the pipe lid adaptive device include substrate, setting puncture needle on the substrate and Circumferential seal set.
In one embodiment, the puncture needle is hollow.
In one embodiment, the pipe lid adaptive device is integrated injection molding.
Pipe lid provided by the invention has the advantage that
(1) Guan Gai and the close fastening of pipe, tolerance high-temperature biological reaction (such as PCR reaction).
(2) Guan Gai is opened and closed convenient with pipe.User opens pipe lid, and required power is small, it is not easy to the biological reagent in generator tube Splash phenomena.
(3) pipe lid is punctured using metal, plastic ware, sample it is convenient.When user uses metal, plastic ware (syringe, liquid-transfering gun) or pipe lid adaptive device are easy to pierce through pipe lid, facilitate the biological reagent in extraction tube.Metal, modeling Glassware tool is good with air-tightness at pipe lid puncture.
Detailed description of the invention
It in order to more clearly explain the technical solutions in the embodiments of the present application, below will be to needed in the embodiment Attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only some embodiments as described in this application, right For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings Its attached drawing.
Fig. 1 is to manage matching used pipe lid arrangement outer surface structure schematic diagram with general 8 platoon EP;
Fig. 2 is to manage matching used pipe lid arrangement inner surface structure schematic diagram with general 8 platoon EP;
Fig. 3 is the structural schematic diagram that single pipe lid and EP pipe thread close;
The pipe lid adaptive device structural schematic diagram that Fig. 4 is matched with the 8 platoon EP pipe pipe lids of Fig. 1;With
Fig. 5 is the schematic diagram that Guan Gai is connect with pipe lid adaptive device.
Specific embodiment
In order to make art technology field personnel more fully understand the technical solution in the application, below in conjunction with following knot Closing embodiment, the invention will be further described, it is clear that and described embodiments are only a part of embodiments of the present application, without It is whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not before making creative work All other embodiment obtained is put, shall fall within the protection scope of the present application.It is right with reference to the accompanying drawings and embodiments The present invention is further described.
In one embodiment, as illustrated in fig. 1 and 2, matching used pipe lid, each pipe lid are managed with general 8 platoon EP Including with flowering structure: carrying out the upper sealing structure 11 of top seal with pipe lid adaptive device, carried out with pipe lid adaptive device circumferential The circumferential closed structure 12 of sealing, thin-walled paracentesis area 13, EP inside pipe wall sealing structure 14 and EP pipe outer wall sealing structure 15.On Sealing structure 11 and circumferential closed structure 12 are located at the outside of pipe lid, and EP inside pipe wall sealing structure 14 and the sealing of EP pipe outer wall are tied Structure 15 is located inside pipe lid, and 13 upper surface of thin-walled paracentesis area is the upper surface and the thin-walled paracentesis area 13 outside pipe lid Upper surface is located within region defined by the upper sealing structure 11, and 13 lower surface of thin-walled paracentesis area is located at inner wall sealing Within region defined by structure 14.
It in some embodiments, is integrated molding for managing matching used pipe lid arrangement with general 8 platoon EP, such as Using the method for compression moulding;It contains 8 pipe lids, and the circumferential closed structure 12 of each pipe lid links together, and is adapted to dress with pipe lid Set carry out circumferential seal.Outer lower is connected together by connector between each pipe lid.
In some embodiments, upper sealing structure 11 is the upper surface projecting annular structure outside pipe lid, and circumferential close Closing structure 12 is the projecting annular structure around pipe lid outer lower.In some embodiments, inner wall sealing structure 14 and outer Wall sealing structure 15 is the cyclic structure cooperated respectively with EP inside pipe wall and outer wall.
In one embodiment, as shown in figure 3, single pipe lid and the connection that EP pipe thread closes are as follows.The inner wall 21 of EP pipe, The EP inside pipe wall sealing structure 14 and EP pipe outer wall sealing structure 15 of outer wall 22 and single pipe lid closely fasten, and are resistant to high-temperature biological Reaction (such as PCR reaction).It is convenient that pipe lid is opened and closed with EP pipe, it is not easy to the biological reagent splash phenomena in EP pipe occur.
In one embodiment, the present invention provides a kind of pipe matched with the 8 platoon EP pipe pipe lid arrangements of Fig. 1 and 2 Lid adaptive device 3.As shown in figure 4, being fixed with 8 plastic puncture needles 32 in the pipe lid adaptive device substrate 31, each plastics are worn Pricker corresponds to the thin-walled paracentesis area 13 of each pipe lid of 8 platoon EP pipes, punctures to it;With 8 plastic puncture needle weeks Be provided in the substrate 31 enclosed annular circumferential seal cover 33, with Fig. 2 and 3 in 8 platoon EP pipes circumferential closed structure 12 into Row cooperation realizes that sealing is fixed.In some embodiments, plastic puncture needle 31 is hollow.
As shown in figure 5, the connection schematic diagram that 8 platoon pipe lid arrangements and pipe lid adaptive device 3 fasten.Plastic puncture needle can be with Easily the thin-walled paracentesis area 13 of pipe lid is realized and is punctured.Pipe covers sealing structure 11 and the top of pipe lid adaptive device 3 is tight Close fastening.Since pipe lid material is silica gel, plastic puncture needle 31 and air-tightness at pipe lid puncture are good.
It should be understood that the present invention disclosed is not limited only to specific method, scheme and the substance of description, because these It is alterable.It will also be understood that purpose of the terminology used here just for the sake of the specific embodiment scheme of description, rather than It is intended to limit the scope of the invention, the scope of the present invention is limited solely by the attached claims.
Those skilled in the art, which will also be appreciated that or be able to confirm that, uses no more than routine experiment, institute herein The many equivalents for the specific embodiment of the invention stated.These equivalents are also contained in the attached claims.

Claims (10)

1. a kind of pipe lid, it is characterised in that: the pipe lid is integrally formed by rubber material;The pipe lid includes being located at outside pipe lid The upper sealing structure of upper surface, inner wall sealing structure and outer wall sealing structure and thin-walled paracentesis area inside the pipe lid; It is located within region defined by the upper sealing structure with thin-walled paracentesis area upper surface, thin-walled penetrating area lower surface position Within the region defined by the inner wall sealing structure.
2. pipe lid according to claim 1, it is characterised in that: the pipe lid includes the closed knot of circumferential direction outside pipe lid Structure.
3. pipe lid according to claim 1, it is characterised in that: the rubber material is silica gel.
4. pipe lid according to claim 1, it is characterised in that: the upper sealing structure is the pipe lid external upper Projecting annular structure and/or the circumferential closed structure are the projecting annular structures around the pipe lid outer lower.
5. pipe lid according to claim 1, it is characterised in that: the inner wall sealing structure and outer wall sealing structure are respectively The cyclic structure cooperated with inside pipe wall and pipe outer wall.
6. a kind of pipe lid arrangement containing multiple -5 any pipe lids according to claim 1, it is characterised in that: the multiple It is attached between pipe lid by attachment device.
7. pipe lid arrangement according to claim 6, it is characterised in that: the circumferential closed structure of adjacent tubes lid is connected to each other to One.
8. a kind of cooperated with any any pipe lid arrangement of the pipe lid and claim 6-7 of claim 1-5 The pipe lid adaptive device used, the pipe lid adaptive device include substrate, setting puncture needle on the substrate and circumferential close Big envelope.
9. pipe lid adaptive device according to claim 8, the puncture needle are hollow.
10. the pipe lid adaptive device is integrated injection molding according to claim 8-9 any pipe lid adaptive device.
CN201711161137.5A 2017-11-20 2017-11-20 A kind of the pipe lid and pipe lid adaptive device used in conjunction with of suitable puncture Pending CN109806922A (en)

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Application Number Priority Date Filing Date Title
CN201711161137.5A CN109806922A (en) 2017-11-20 2017-11-20 A kind of the pipe lid and pipe lid adaptive device used in conjunction with of suitable puncture

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Publication Number Publication Date
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112619718A (en) * 2020-11-17 2021-04-09 浙江省海洋水产研究所 Exempt from to uncap centrifuging tube
CN113897277A (en) * 2021-10-15 2022-01-07 圣湘生物科技股份有限公司 Reaction apparatus and control method thereof

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JPH0824313A (en) * 1994-07-21 1996-01-30 Otsuka Pharmaceut Factory Inc Container for transfusion
JP2003111819A (en) * 2001-07-30 2003-04-15 Otsuka Pharmaceut Factory Inc Sealing body, cap for medicine container using the sealing body, and medicine container
US20070187353A1 (en) * 2006-02-13 2007-08-16 Tripath Imaging, Inc. Container assembly and pressure-responsive penetrable cap for the same
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CN202226864U (en) * 2011-09-23 2012-05-23 安徽农业大学 Microcentrifugal tube
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WO2014165559A2 (en) * 2013-04-02 2014-10-09 Raindance Technologies, Inc. Systems and methods for handling microfluidic droplets
CN204529859U (en) * 2015-01-21 2015-08-05 钟灿秋 PCR pipe lid
WO2016003543A1 (en) * 2014-07-02 2016-01-07 Taunk Dale Singh Ergonomic numbered connector to hold tubes with improved cap
CN105498879A (en) * 2016-03-05 2016-04-20 张洪美 Centrifugal tube
CN207722821U (en) * 2017-11-20 2018-08-14 北京天健惠康生物科技有限公司 A kind of pipe lid of suitable puncture and the pipe lid adaptive device being used cooperatively with it

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2069116U (en) * 1990-02-09 1991-01-09 俞志飞 Centrifugal tube for physical analysis of biological material
JPH0824313A (en) * 1994-07-21 1996-01-30 Otsuka Pharmaceut Factory Inc Container for transfusion
JP2003111819A (en) * 2001-07-30 2003-04-15 Otsuka Pharmaceut Factory Inc Sealing body, cap for medicine container using the sealing body, and medicine container
US20070187353A1 (en) * 2006-02-13 2007-08-16 Tripath Imaging, Inc. Container assembly and pressure-responsive penetrable cap for the same
CN101415615A (en) * 2006-02-13 2009-04-22 三路影像公司 Container assembly and pressure-responsive penetrable cap for the same
US20100113976A1 (en) * 2007-05-25 2010-05-06 Roche Diagnostics Operations, Inc. Sealing cap for a body fluid container and a blood collection device
CN202226864U (en) * 2011-09-23 2012-05-23 安徽农业大学 Microcentrifugal tube
CN102836753A (en) * 2012-09-26 2012-12-26 无锡耐思生物科技有限公司 Centrifuge tube structure
WO2014165559A2 (en) * 2013-04-02 2014-10-09 Raindance Technologies, Inc. Systems and methods for handling microfluidic droplets
WO2016003543A1 (en) * 2014-07-02 2016-01-07 Taunk Dale Singh Ergonomic numbered connector to hold tubes with improved cap
CN204529859U (en) * 2015-01-21 2015-08-05 钟灿秋 PCR pipe lid
CN105498879A (en) * 2016-03-05 2016-04-20 张洪美 Centrifugal tube
CN207722821U (en) * 2017-11-20 2018-08-14 北京天健惠康生物科技有限公司 A kind of pipe lid of suitable puncture and the pipe lid adaptive device being used cooperatively with it

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112619718A (en) * 2020-11-17 2021-04-09 浙江省海洋水产研究所 Exempt from to uncap centrifuging tube
CN112619718B (en) * 2020-11-17 2022-06-14 浙江省海洋水产研究所 Exempt from to uncap centrifuging tube
CN113897277A (en) * 2021-10-15 2022-01-07 圣湘生物科技股份有限公司 Reaction apparatus and control method thereof
CN113897277B (en) * 2021-10-15 2023-12-01 圣湘生物科技股份有限公司 Reaction apparatus and control method thereof

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