CN109799189A - A kind of centrifugal cycle detection pond and its application - Google Patents
A kind of centrifugal cycle detection pond and its application Download PDFInfo
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- CN109799189A CN109799189A CN201910068403.2A CN201910068403A CN109799189A CN 109799189 A CN109799189 A CN 109799189A CN 201910068403 A CN201910068403 A CN 201910068403A CN 109799189 A CN109799189 A CN 109799189A
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- 238000001514 detection method Methods 0.000 title claims abstract description 85
- 210000002421 cell wall Anatomy 0.000 claims description 29
- 238000005119 centrifugation Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 abstract description 69
- 239000012472 biological sample Substances 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 5
- 230000002906 microbiologic effect Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 17
- 238000013461 design Methods 0.000 description 12
- 238000001237 Raman spectrum Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 102000004330 Rhodopsin Human genes 0.000 description 7
- 108090000820 Rhodopsin Proteins 0.000 description 7
- NCYCYZXNIZJOKI-IOUUIBBYSA-N 11-cis-retinal Chemical compound O=C/C=C(\C)/C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-IOUUIBBYSA-N 0.000 description 6
- 238000001069 Raman spectroscopy Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009659 non-destructive testing Methods 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000007616 round robin method Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000005469 synchrotron radiation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Centrifugal Separators (AREA)
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Abstract
The invention discloses a kind of centrifugal cycle detection ponds, including bottom of pond and side wall, side wall to be carved with annular groove.Above-mentioned centrifugal cycle detection pond is able to satisfy the testing requirements of various micro biological samples and expensive rare sample, suitable for detecting micro sensibility solution example characteristic signal using probing waves such as laser or rays, it can avoid sample when detection to be damaged, structure is simple, easy to operate.The invention also discloses a kind of application of above-mentioned centrifugal cycle detection pond in probing wave detection micro-example, sample is added in centrifugal cycle detection pond, centrifugal cycle detection pond is at the uniform velocity rotated using central axis as rotary shaft, because centrifugal action is distributed in annular groove, probing wave reflects to obtain echo signal sample through the sample in annular groove.
Description
Technical field
The present invention relates to detection device technical fields, and in particular to a kind of centrifugal cycle detection pond and its application.
Background technique
With the development of science and technology, various instrument and equipments and detection technique emerge one after another.Synchrotron radiation and high energy particle
The application of stream, the requirement to test sample are also increasingly harsher.Such as it is drawn in the resonance for collecting biological sample using ultraviolet laser
When graceful spectrum, sample is needed to be dissolved in a large amount of solvents, is flowed using mechanical pump by cycles samples, otherwise ultraviolet laser it is long when
Between irradiation can destroy the activity of biological sample.It is very expensive or very rare which limits the extensive use of the detection technique
Sample, or the sample of recycle stream momentum cannot be provided cannot all detect.
The patent specification of Publication No. CN101806726A discloses a kind of for the absorption of micro liquid ultraviolet double-wavelength
Photometric detection device passes through optical path by light source, liquid core waveguide sample detection cell, photoelectric detector, control four part of detection circuit
It is constituted with connection.Light source is made of the UV LED of two different wave lengths.Sample detection cell is by a wick-containing wave
Conduit is constituted, and two nozzles of liquid core waveguide pipe open introducing and extraction for being analyzed solution.There are two liquid core waveguide pipe is set
Incident photo-coupler and outgoing photo-coupler of the turn region as liquid core waveguide sample detection cell.Control detection circuit timesharing point
Bright two LED sources are respectively lighting constantly light intensity of the detection through liquid core waveguide detection cell respectively by detector
Degree obtains absorbance of the fluid to be measured under two wavelength.Above-mentioned apparatus realizes the ultraviolet double-wavelength absorbance of micro liquid
Detection, sample consumption is few, be easy to be miniaturized and it is integrated.
The patent specification of Publication No. CN107607446A discloses a kind of internal-circulation type micro-example pond, including driving
Sample window upper cover and sample window bottom is respectively set in device, agitating device, sample window and fixed bracket, sample window upper and lower ends
Seat, sample window pedestal are fixed on fixed bracket, and counter sample window central location is equipped with light hole, the sample on fixed bracket
The flow deflector of L-type is equipped between the front and back two sides of window, the flow deflector of L-type is integrally divided into mutually perpendicular riser and transverse sheet, described
Riser and transverse sheet are vertical with optical path, and riser is parallel with sample window side, and agitating device is located at the cavity of resorption of flow deflector transverse sheet lower part
In vivo, and driven device driving rotates clockwise.Above-mentioned sample cell takes the mode of interior circulation to recycle solution, fits
It is easy to operate for the detection of micro-example.
Device disclosed in above-mentioned patent specification is more complicated, thus design it is a kind of simply suitable for micro-example but
The detection method and detection device of energy loop test are a vital problems again, have also attracted more and more experiment skills
The professional of art related fields and the interest of researcher.
Summary of the invention
For shortcoming existing for this field, the present invention provides a kind of centrifugal cycle detection ponds (referred to as to detect
Pond), the testing requirements of various micro biological samples and expensive rare sample are able to satisfy, are suitable for utilizing the detection such as laser or ray
Wave detects micro sensibility solution example characteristic signal, can avoid sample when detection and is damaged, structure is simple, easy to operate.
A kind of centrifugal cycle detection pond, including bottom of pond and side wall, the side wall are carved with annular groove.
Preferably, the angle of the bottom of pond and side wall is 90 °~160 °, and the Chi Kou for the detection cell being designed in this way is not less than
Bottom of pond, detection cell can make when rotating sample in detection cell under the action of the centrifugal more easily on move up into annular groove.
Preferably, the slot bottom of the annular groove is horizontal by 5 °~170 °.
Preferably, the upper cell wall of the annular groove and the angle of slot bottom are 10 °~170 °, and the angle of lower cell wall and slot bottom is
10 °~180 °.Upper cell wall, lower cell wall and slot bottom form the sample that certain angle is conducive to when detection cell rotates and store.
Preferably, the upper cell wall of the annular groove is not less than the 3/4 of Sidewall Height away from the height of bottom of pond.Ring groove position design
It is higher, it is possible to reduce the order of reflection of probing wave when detection, convenient for the reflection and acquisition of probing wave.Preferably, groove width is side wall
The 1/40~1/10 of height, the depth of upper cell wall are the 1/5~2/3 of sidewall thickness, and the depth of lower cell wall is not more than sidewall thickness
4/5.Annular groove design is excessive, and the sample needed just will increase.Annular groove design is smaller, may be implemented micro when detection cell rotation
Sample can be uniformly distributed full annular groove.The groove width and depth of annular groove can be specifically designed according to actual needs.
Preferably, the annular groove is arc groove, and radius of curvature is 0.05~200cm, height of the edge away from bottom of pond on annular groove
Not less than the 3/4 of Sidewall Height.For arc groove there is no sample flow dead angle, sample flow is more preferable, is more evenly distributed.Annular groove
Position Design is higher, it is possible to reduce the order of reflection of probing wave when detection, convenient for the reflection and acquisition of probing wave.Preferably, ring
Groove depth is the 1/5~4/5 of sidewall thickness, and groove width is the 1/40~1/10 of Sidewall Height.Annular groove designs excessive, the sample needed
Just it will increase.Annular groove design is smaller, and micro sample can be uniformly distributed full annular groove when detection cell rotation may be implemented.Specifically may be used
The groove width and depth of annular groove are designed according to actual needs.
Preferably, the bottom of pond is circle, and centrifugal cycle detection pond is up big and down small truncated cone-shaped structure, is conducive to inspection
Sample moves up into annular groove when surveying pond rotation under the action of the centrifugal, and is uniformly distributed in annular groove.
The pond mouthful in centrifugal cycle detection pond can be open design, pond mouthful 1~30cm of diameter.
Preferably, the bottom of pond 1~20cm of diameter, 0.1~1cm of sidewall thickness, 1~20cm of Sidewall Height.Described
Bottom of pond thickness can be 0.1~1cm.The detection cell being designed in this way detects micro-example enough, does not waste material.Moreover, inspection
Survey that pond is smaller, when centrifugal rotation it is required power and energy it is also less.The upper cell wall of the annular groove away from the height of Chi Kou not
Greater than 5cm, the angle of upper cell wall and slot bottom is 10 °~170 °, and the angle of lower cell wall and slot bottom is 10 °~180 °, slot bottom and water
Plane is in 5 °~170 °.Annular groove groove width be 0.1~2cm, upper cell wall depth be 0.02~0.5cm, lower cell wall depth be 0.02~
0.8cm。
The annular groove can also be arc groove, and radius of curvature is 0.05~200cm, and groove depth is 0.02~0.8cm, and groove width is
0.1~2cm.
The present invention also provides the centrifugal cycle detection ponds described in one kind to detect the application in micro-example in probing wave,
Sample is added in centrifugal cycle detection pond, centrifugal cycle detection pond is at the uniform velocity rotated using central axis as rotary shaft, sample
Because centrifugal action is distributed in annular groove, probing wave reflects to obtain echo signal product through the sample in annular groove.
Centrifugal cycle detection pond, which quickly rotates, is distributed in sample centrifugation in annular groove, by adjusting rotation angle, revolving speed
And probing wave incoming position, so that probing wave is irradiated to sample.Since sample is with detection cell high speed rotation, visited so that sample is in
It surveys the circulation of wave, be spaced irradiation, rather than prolonged exposure, guarantee that sample there are enough relaxation times and will not be destroyed.
The revolving speed of the centrifugal cycle detection pond rotation can be 100~6000rpm, can be determined according to properties of samples
Suitable revolving speed.Revolving speed is too small, and the centrifugal action that sample is subject to is too small and cannot be introduced into annular groove, or is unevenly distributed in annular groove
It is even.Revolving speed is excessive, and the centrifugal action that sample is subject to is too big, is easy to splash, leads to the waste of sample.
Rotary shaft and horizontal angle when the centrifugal cycle detection pond rotates can be 0 °~90 °.Some
In the case of, detection cell needs to rotate after tilting, so that probing wave does not need the accessories such as reflecting mirror, can shine directly into sample, make
Sample reflection signal obtains echo signal.Component can be saved in this way, and is easier adjustment probing wave route.
Compared with prior art, the present invention major advantage includes:
(1) the centrifugal cycle detection pond described in is able to satisfy various micro biological samples and the detection of expensive rare sample is wanted
It asks, is suitable for detecting micro sensibility solution example characteristic signal using probing waves such as laser or rays, can avoid sample when detection
Product are impaired, and structure is simple, easy to operate.
(2) application described in is simple and efficient, and the circulation that sample can be made to be in incidence wave is irradiated rather than prolonged exposure, is protected
It demonstrate,proves the enough relaxation times of sample and will not be destroyed.
(3) have a wide range of application, such as the Raman spectrum of the detection of micro or even trace samplings, photosensitive sample is detected and synchronized
The application of irradiating biological sample detection etc..
Detailed description of the invention
Fig. 1 is the structural schematic diagram in the centrifugal cycle detection pond of embodiment 1；
Fig. 2 is that the centrifugal cycle detection pond of embodiment 1 is applied to the photo in kind of the micro non-destructive testing of Raman spectrum；
Fig. 3 is the Raman signal (a) of embodiment 1 and Raman signal (b) figure of comparative example 2；
Fig. 4 is the structural schematic diagram in the centrifugal cycle detection pond of embodiment 2；
Fig. 5 is the structural schematic diagram in the centrifugal cycle detection pond of embodiment 3；
Fig. 6 is the structural schematic diagram in the centrifugal cycle detection pond of embodiment 4；
In figure: 1- reflecting mirror；2- sample；3- probing wave；4- rotary shaft；The centrifugal cycle detection pond 5-；6- convex lens.
Specific embodiment
With reference to the accompanying drawing and specific embodiment, the present invention is further explained.It should be understood that the embodiment of the present invention is only used for
Illustrate the present invention rather than limits the scope of the invention.The method that actual conditions are not specified in the following example, usually according to
Normal condition, or according to the normal condition proposed by manufacturer.
Embodiment 1
Centrifugal cycle detection pond 5 as shown in Figure 1 is the up big and down small hollow round table knot surrounded by bottom of pond and side wall
Structure, Chi Kou are open design.Bottom of pond and side wall angle are 100 °, bottom of pond diameter 4.6cm, pond mouthful diameter 6cm, Sidewall Height 4cm,
Side thickness 0.3cm.
Side wall is carved with annular groove, and the upper cell wall of annular groove is away from pond mouthful 0.2cm, and annular groove and pond mouthful plane, bottom of pond are parallel, annular groove slot
Horizontal by 90 °, upper cell wall and slot bottom angle are 90 ° at bottom, and lower cell wall and slot bottom angle are 90 °.Annular groove groove width is 0.3cm,
Upper cell wall depth is 0.15cm, lower cell wall depth 0.15cm.
When using above-mentioned centrifugal cycle detection pond 5, using rotary table central axis as 4 uniform rotation of rotary shaft, revolving speed 1800
Rev/min.Rotary table rotary shaft and horizontal line angle are 90 °.Probing wave 3 is irradiated to sample 2 in annular groove by reflecting mirror 1, through sample
Signal wave after product 2 reflect is collected by reflecting mirror 1 and the convergence of convex lens 6 and obtains echo signal.
As shown in Fig. 2, above-mentioned centrifugal cycle detection pond 5 is detected applied to Raman spectrum, the rhodopsin albumen of 0.2mL
It is put in detection cell, drives detection cell with 1800 revs/min of high speed rotations by motor, sample is distributed to ring under the action of the centrifugal
Is rotated together with detection cell in slot, using wavelength for 196nm laser as probing wave, be incident to sample in annular groove, collection is through sample
The signal that product scatter, the as Raman spectrum of rhodopsin albumen are collected and obtain within 20 minutes Raman letter as shown in Figure 3a
Number.
Comparative example 1
Rhodopsin albumen Raman spectrum is detected using traditional static quartz colorimetric utensil, takes 2mL sample in quartz cuvette
In ware, using wavelength for 196nm laser as probing wave, be incident to cuvette, collect the signal scattered through sample, as
The Raman spectrum of rhodopsin albumen is collected and observes within 5 minutes sample discoloration coagulation phenomenon, is unable to get Raman signal.
Comparative example 2
Sample solution round-robin method detection rhodopsin albumen Raman spectrum is moved using peristaltic pump, takes 50mL sample in taper
Bottle in, using wavelength for 196nm laser be used as probing wave, be incident to solution circulation stable state liquid stream in, collect scattered through sample
The signal arrived, the as Raman spectrum of rhodopsin albumen are collected 20 minutes and obtain Raman signal as shown in Figure 3b.
Since this method can only survey the solution of a large amount of samples, and the sources such as rhodopsin protein mutant are extremely limited
Sample, cannot accomplish to circulate and testing goal is not achieved.
Embodiment 2
Centrifugal cycle detection pond 5 as shown in Figure 4 is the up big and down small hollow round table knot surrounded by bottom of pond and side wall
Structure, Chi Kou are open design.Bottom of pond and side wall angle are 95 °, bottom of pond diameter 5.3cm, pond mouthful diameter 6cm, Sidewall Height 4cm,
Side thickness 0.3cm.
Side wall is carved with annular groove, and the upper cell wall of annular groove is away from pond mouthful 0.2cm, and annular groove and pond mouthful plane, bottom of pond are parallel, annular groove slot
Horizontal by 100 °, upper cell wall and slot bottom angle are 110 ° at bottom, and lower cell wall and slot bottom angle are 180 °.Annular groove groove width is
3.8cm, upper cell wall depth are 0.2cm, lower cell wall depth 0cm.
When using above-mentioned centrifugal cycle detection pond 5, using rotary table central axis as 4 uniform rotation of rotary shaft, revolving speed 1700
Rev/min.Rotary table rotary shaft and horizontal line angle are 90 °.Probing wave 3 is irradiated to sample 2 in annular groove by reflecting mirror 1, through sample
Signal wave after product 2 reflect is collected by reflecting mirror 1 and the convergence of convex lens 6 and obtains echo signal.
Embodiment 3
Centrifugal cycle detection pond 5 as shown in Figure 5 is the up big and down small hollow round table knot surrounded by bottom of pond and side wall
Structure, Chi Kou are open design.Bottom of pond and side wall angle are 95 °, bottom of pond diameter 5.3cm, pond mouthful diameter 6cm, Sidewall Height 4cm,
Side thickness 0.3cm.
Side wall is carved with annular groove, and the upper cell wall of annular groove is away from pond mouthful 0.1cm, and annular groove and pond mouthful plane, bottom of pond are parallel, annular groove slot
Horizontal by 60 °, upper cell wall and slot bottom angle are 120 ° at bottom, and lower cell wall and slot bottom angle are 70 °.Annular groove groove width is 0.3cm,
Upper cell wall depth is 0.15cm, lower cell wall depth 0.15cm.
When using above-mentioned centrifugal cycle detection pond 5, using rotary table central axis as 4 uniform rotation of rotary shaft, revolving speed 2000
Rev/min.Rotary table rotary shaft and horizontal line angle are 70 °.Probing wave 3 is irradiated to sample 2 in annular groove by reflecting mirror 1, through sample
Signal wave after product 2 reflect is collected by reflecting mirror 1 and the convergence of convex lens 6 and obtains echo signal.
Embodiment 4
Centrifugal cycle detection pond 5 as shown in FIG. 6 is the up big and down small hollow round table knot surrounded by bottom of pond and side wall
Structure, Chi Kou are open design.Bottom of pond and side wall angle are 100 °, bottom of pond diameter 4.6cm, pond mouthful diameter 6cm, Sidewall Height 4cm,
Side thickness 0.3cm.
Side wall is carved with annular groove, and annular groove is arc groove, radius of curvature 0.5cm.Edge is away from pond mouthful 0.1cm, depth on annular groove
0.2cm, groove width 0.4cm.
When using above-mentioned centrifugal cycle detection pond 5, using rotary table central axis as 4 uniform rotation of rotary shaft, revolving speed 1800
Rev/min.Rotary table rotary shaft and horizontal line angle are 90 °.Probing wave 3 is irradiated to sample 2 in annular groove by reflecting mirror 1, through sample
Signal wave after product 2 reflect is collected by reflecting mirror 1 and the convergence of convex lens 6 and obtains echo signal.
In addition, it should also be understood that, those skilled in the art can be to this hair after having read foregoing description content of the invention
Bright to make various changes or modifications, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (10)
1. a kind of centrifugal cycle detection pond, including bottom of pond and side wall, which is characterized in that the side wall is carved with annular groove.
2. centrifugal cycle detection pond according to claim 1, which is characterized in that the angle of the bottom of pond and side wall is
90 °~160 °.
3. centrifugal cycle detection pond according to claim 1, which is characterized in that the upper cell wall and slot bottom of the annular groove
Angle be 10 °~170 °, the angle of lower cell wall and slot bottom is 10 °~180 °.
4. centrifugal cycle detection pond according to claim 1, which is characterized in that the upper edge of the annular groove is away from bottom of pond
Height is not less than the 3/4 of Sidewall Height.
5. centrifugal cycle detection pond according to claim 1 or 4, which is characterized in that the annular groove is arc groove, bent
Rate radius is 0.05~200cm.
6. centrifugal cycle detection pond according to claim 1 or 2, which is characterized in that the bottom of pond is circle, centrifugation
Formula cycle detection pond is up big and down small truncated cone-shaped structure.
7. centrifugal cycle detection pond according to claim 6, which is characterized in that the bottom of pond 1~20cm of diameter, side
0.1~1cm of wall thickness, 1~20cm of Sidewall Height.
8. centrifugal cycle detection pond described in a kind of any claim according to claim 1~7 is detected micro in probing wave
Application in sample, which is characterized in that sample is added in centrifugal cycle detection pond, centrifugal cycle detection pond is in itself
Mandrel is that rotary shaft at the uniform velocity rotates, and because centrifugal action is distributed in annular groove, probing wave reflects to obtain sample through the sample in annular groove
Echo signal.
9. application according to claim 8, which is characterized in that the revolving speed of the described centrifugal cycle detection pond rotation is
100~6000rpm.
10. application according to claim 8 or claim 9, which is characterized in that rotation when the centrifugal cycle detection pond rotates
Shaft and horizontal angle are 0 °~90 °.
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| CN201910068403.2A CN109799189B (en) | 2019-01-24 | 2019-01-24 | Centrifugal circulation detection pool and application thereof |
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|---|---|---|---|---|
| JPS5654355A (en) * | 1979-10-09 | 1981-05-14 | Olympus Optical Co Ltd | Cohesion reaction measuring method and reacting vessel used for it |
| EP0123178A2 (en) * | 1983-04-13 | 1984-10-31 | INSTRUMENTATION LABORATORY S.p.A. | Use of an analytical photometer, of centrifugal type, for the practically simultaneous determination of the presence of different substances in a certain number of discrete samples |
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| CN109799189B (en) | 2021-10-08 |
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