CN109797185A - Application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis - Google Patents
Application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis Download PDFInfo
- Publication number
- CN109797185A CN109797185A CN201910066687.1A CN201910066687A CN109797185A CN 109797185 A CN109797185 A CN 109797185A CN 201910066687 A CN201910066687 A CN 201910066687A CN 109797185 A CN109797185 A CN 109797185A
- Authority
- CN
- China
- Prior art keywords
- methyl phenyl
- carbinyl acetate
- phenyl carbinyl
- bacillus
- catalysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis.The present invention is using the full cell of the Bacillus sp.DL-2 separated from Western Pacific's halmeic deposit as catalyst, it can be catalyzed (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis, can be used for preparing chiral (R) -1- benzyl carbinol and (S)-methyl phenyl carbinyl acetate.The present invention is using the full cell of Bacillus sp.DL-2 as catalyst, reaction system and condition are optimized, be prepared optical purity be 96% (R) -1- benzyl carbinol and optical purity be up to 99.8% (S)-methyl phenyl carbinyl acetate.The full cell of Bacillus sp.DL-2 of the invention has the advantages that simple production process, environmental-friendly, high catalytic efficiency as biocatalyst, has biggish potentiality to be exploited in the industrial production of fine chemicals.
Description
Technical field:
The invention belongs to biochemical industries and field of biotechnology, and in particular to a kind of from Western Pacific's halmeic deposit sample
The full cell of middle isolated bacillus sp.DL-2 is in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis
Using.
Background technique:
The preparation of chiral 1- benzyl carbinol and its derivative is in the industrial production in occupation of critical role, because of its optically pure list
Body molecule is the key intermediate for synthesizing many drugs and chemicals.Traditional chemical synthesizes 1- benzyl carbinol and its derivative, tool
Have expensive reagents, severe reaction conditions, it is at high cost, pollution it is big, time-consuming the problems such as.In contrast, biological catalysis has specifically
The advantages that property is strong, reaction condition is mild, easy to operate, at low cost, environmentally friendly.Use microbe whole-cell as catalyst
There is unique advantage in industrial applications.On the one hand, microorganism is directly participated in catalysis reaction, point of enzyme can be saved
From cumbersome steps such as, purifying, immobilizations, production cost is greatly reduced, while caused by also avoiding in those steps
Enzyme activity loss.On the other hand, cell is the natural carrier of enzyme, can in non-aqueous media protective enzyme, reduce non-aqueous media to enzyme
Caused vigor loss, the stability of enzyme will greatly improve.It is urged in addition, being conducive to multienzyme as biocatalyst using full cell
Change or need the biotransformation of coenzyme.
Summary of the invention:
The purpose of the present invention is overcoming defect in the prior art, provide in a kind of Western Pacific's halmeic deposit sample point
From bacillus sp.DL-2 full cell answering in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis
With.
Bacillus sp.DL-2 of the invention was preserved in Guangdong Province microorganism fungus kind on September 12nd, 2018
Collection (GDMCC), address: 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, deposit number are as follows: GDMCC1.1556.
The bacterial strain is open preservation, and in Guangdong Province, Culture Collection is for sale.
The present invention is separated to a bacillus Bacillus sp.DL-2 from Western Pacific's halmeic deposit, utilizes
The full cell of Bacillus sp.DL-2 can be catalyzed (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis, for making as catalyst
Standby chirality (R) -1- benzyl carbinol and (S)-methyl phenyl carbinyl acetate.
Therefore, the purpose of the present invention is to provide the full cells of bacillus sp.DL-2 in catalysis (±)-second
Application in sour styracin asymmetric hydrolysis.
It is preferred that the full cell of the bacillus sp.DL-2 splits (±)-acetic acid storax in catalysis
Ester obtains the application in (R) -1- benzyl carbinol.
It is preferred that it is in metal ion, surface that the catalysis, which splits (±)-methyl phenyl carbinyl acetate to obtain (R) -1- benzyl carbinol,
Activating agent or organic solvent, which are deposited, at ambient to carry out.
The metal ion is preferably Na+、K+、Co2+、Cu2+、Mg2+、Mn2+Or Ni2+；The surfactant is preferred
For cetyl trimethylammonium bromide or sodium carboxymethylcellulose；The organic solvent is preferably acetone, ethyl alcohol, methanol or N,
Dinethylformamide.
The reaction system that described catalysis fractionation (±)-methyl phenyl carbinyl acetate obtains (R) -1- benzyl carbinol is preferred are as follows: including
Bacillus sp.DL-2,5mM substrate (±)-acetic acid Soviet Union that cell concentration is calculated as 32mg/mL with wet cell weight closes
The n,N-Dimethylformamide of fragrant ester and volume fraction 5%, remaining is the sodium phosphate buffer of pH6.0；Reaction condition are as follows: 40 DEG C
React 4h.
It is preferred that the full cell of the bacillus sp.DL-2 splits (±)-acetic acid storax in catalysis
Ester obtains the application in (S)-methyl phenyl carbinyl acetate.
It is preferred that the catalysis split (±)-methyl phenyl carbinyl acetate obtain (S)-methyl phenyl carbinyl acetate be metal ion,
Surfactant or organic solvent, which are deposited, at ambient to carry out.
The metal ion is preferably K+、Ca2+、Co2+、Mg2+、Mn2+、Zn2+、Al3+Or Fe3+；The surface-active
Agent is preferably Tween-20, TritonX-100, Span 65, cetyl trimethylammonium bromide or sodium carboxymethylcellulose；Institute
The organic solvent stated is preferably dimethyl sulfoxide.
The reaction system that described catalysis fractionation (±)-methyl phenyl carbinyl acetate obtains (S)-methyl phenyl carbinyl acetate is preferred are as follows:
Bacillus sp.DL-2,2.5mM substrate (±)-second of 32mg/mL is calculated as with wet cell weight including cell concentration
Sour styracin and 2mM Al3+, remaining is the sodium phosphate buffer of pH7.5；Reaction condition are as follows: 40 DEG C of reaction 4h.
The present invention is separated to one plant of marine microorganism bacillus from Western Pacific's halmeic deposit
Sp.DL-2 can be catalyzed (±)-acetic acid Soviet Union and close using the full cell of bacillus sp.DL-2 as catalyst
Fragrant ester asymmetric hydrolysis can be used for preparing chiral (R) -1- benzyl carbinol and (S)-methyl phenyl carbinyl acetate.The present invention utilizes gemma bar
The full cell of bacterium Bacillus sp.DL-2 optimizes reaction system and reaction condition, is prepared as catalyst
(R) -1- benzyl carbinol (conversion ratio 21%, yield 41%) and optical purity that optical purity is 96% are up to 99.8% (S)-second
Sour styracin (conversion ratio 68%, yield 65%).The full cell of Bacillus sp.DL-2 of the invention is as biocatalysis
Agent has the advantages that simple production process, environmental-friendly, high catalytic efficiency, has in the industrial production of fine chemicals larger
Potentiality to be exploited.
Detailed description of the invention:
Fig. 1 is the growth curve of Bacillus sp.DL-2.
Fig. 2 is the influence that pH splits that (±)-methyl phenyl carbinyl acetate prepares (R) -1- benzyl carbinol to full cell.
Fig. 3 is the influence that temperature splits that (±)-methyl phenyl carbinyl acetate prepares (R) -1- benzyl carbinol to full cell.
Fig. 4 is e.e.pWith C with the change curve of cell concentration.
Fig. 5 is e.e.pWith C with the change curve of concentration of substrate.
Fig. 6 is e.e.pWith C with the change curve in reaction time.
Fig. 7 is the influence that pH splits that (±)-methyl phenyl carbinyl acetate prepares (S)-methyl phenyl carbinyl acetate to full cell.
Fig. 8 is the influence that temperature splits that (±)-methyl phenyl carbinyl acetate prepares (S)-methyl phenyl carbinyl acetate to full cell.
Fig. 9 is e.e.sWith C with the change curve of cell concentration.
Figure 10 is e.e.sWith C with the change curve of concentration of substrate.
Figure 11 is e.e.sWith C with the change curve in reaction time.
Figure 12 is the gas chromatogram of (±) methyl phenyl carbinyl acetate He (±) -1- benzyl carbinol.
Figure 13 is that full cell generates high optical voidness to the selective hydrolysis of (±)-methyl phenyl carbinyl acetate under optimum reaction conditions
(R) -1- benzyl carbinol gas chromatogram.
Figure 14 is that solve bloom to the optional water of (±)-methyl phenyl carbinyl acetate pure for full cell under optimum reaction conditions
(S)-methyl phenyl carbinyl acetate gas chromatogram.
Figure 15 is that full cell Hydrolysis Resolution (±)-methyl phenyl carbinyl acetate of Bacillus sp.DL-2 prepares (R) -1- benzene second
Alcohol 1) and (S)-methyl phenyl carbinyl acetate 2).
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Bacillus sp.DL-2 of the invention is screened from Western Pacific's halmeic deposit and is got and save
In Chinese Academy of Science Nanhai Ocean Research Institute's torrid zone living marine resources and ecological key lab.
Embodiment 1: the identification of Bacillus
The bacillus sp.DL-2 bacterial strain of screening after purification utilizes its conservative 16S rRNA gene order
Its strain is identified.With 16S rRNA universal primer 27F:5'-AGAGTTTATCCTGGCTCAG-3'；And 1492R:5'-
GGTTACCTTGTTACGACTT-3' carries out PCR expansion to it using bacillus sp.DL-2 bacterium solution as DNA profiling
Increase.Establish reaction system as shown in table 1:
Table 1PCR reaction system
PCR amplification condition: 94 DEG C of initial denaturation 10min, then 94 DEG C of denaturation 30s, 56 DEG C of annealing 30s, 72 DEG C of extensions
2min30s is recycled 30 times；Last 72 DEG C of extensions 10min, is cooled to 18 DEG C.Amplified production through 1% agarose gel electrophoresis, into
Row detection is then sent to Mei Ji Bioisystech Co., Ltd and carries out bidirectional sequencing.The 16S rRNA gene order for obtaining the bacterium is spelled
(its nucleotide sequence is as shown in SEQ ID NO.1) identifies the bacterial strain using BLAST and is bacillus and is named as after connecing
Bacillus sp.DL-2, the bacterium were preserved in Guangdong Province's Culture Collection (GDMCC), ground on September 12nd, 2018
Location: 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100, deposit number are as follows: GDMCC 1.1556.The bacterial strain is open preservation,
In Guangdong Province, Culture Collection is for sale.
The growth curve of embodiment 2:Bacillus sp.DL-2
By the bacillus sp.DL-2 of preservation be crossed to LB solid medium (1% tryptone, 0.5%
Yeast powder, 1%NaCl, 1.5% agar powder) on, 37 DEG C of culture 16h, then picking single colonie is inoculated into the LB liquid training of 100mL
It supports in base (1% tryptone, 0.5% yeast powder, 1%NaCl), 200r/min, 37 DEG C of culture 12h obtain seed liquor, by 1%
Inoculum concentration be inoculated in LB liquid medium and cultivate, 200r/min, 37 DEG C of cultures measure its biomass OD- every 2h
600nm, as a result as shown in Figure 1.
The preparation of the full cell of embodiment 3:Bacillus sp.DL-2
The seed liquor of Bacillus sp.DL-2 is inoculated in skimmed milk power fluid nutrient medium by 1% inoculum concentration to send out
Ferment culture, 200r/min, 37 DEG C of cultures for 24 hours (culture medium becomes clarification), obtain fermentation liquid, and supernatant is removed in centrifugation, and cell precipitation is used
It is complete up to Bacillus sp.DL-2 that cell precipitation is collected by centrifugation three times in Tris/HCl buffer washing after pH=7.2 sterilizing
Cell.
Embodiment 4: full cell splits (±)-methyl phenyl carbinyl acetate and prepares (R) -1- benzyl carbinol
4.1pH splits the influence of (±)-methyl phenyl carbinyl acetate to full cell
In the full cell of Bacillus sp.DL-2 that cell concentration is 32mg/mL (wet cell weight, WCW), 50mM, pH are
(±)-methyl phenyl carbinyl acetate that 5mM is added in the buffer solution of sodium phosphate of 6.0-8.0 reacts 4h at 40 DEG C with 200rpm, uses
Gas chromatography chiral post detection, according to calculated by peak area product alcohol enantiomeric excess value (e.e.p) and the substrate transformation rate (C), knot
Fruit sees Fig. 2.
Formula 1:Formula 2:
RnolAnd SnolRespectively indicate the peak area of (R) -1- benzyl carbinol He (S) -1- benzyl carbinol.R0And S0Respectively indicate reaction
The concentration of substrate of preceding corresponding configuration；R and S corresponds to the concentration of substrate of configuration after respectively representing reaction.
As can be seen from Figure 2 in the sodium phosphate buffer of 50mM (PB) pH 6.0, the enantiomeric excess value highest of product
It is 95.4%, the substrate transformation rate is 18.9% at this time.Therefore the 50mM PB of pH 6.0 is suitble to cell to split the conjunction of 5mM (±)-acetic acid Soviet Union
Fragrant ester obtains (R) -1- benzyl carbinol of high-optical-purity.
4.2 temperature split the influence of (±)-methyl phenyl carbinyl acetate to full cell
It is anti-in the PB (50mM) of the pH 6.0 for the full cell of Bacillus sp.DL-2 that cell concentration is 32mg/mL (WCW)
Addition 5mM substrate (±)-methyl phenyl carbinyl acetate in system is answered, in different temperatures (20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C, 45
DEG C, 50 DEG C) under with 200rpm react 4h, with gas chromatography chiral post detection and calculate e.e.pAnd C, as a result see Fig. 3.Show temperature
When degree is 40 DEG C, full cell is best to the fractionation effect of (±)-methyl phenyl carbinyl acetate, the enantiomeric excess value of product alcohol and bottom
Object conversion ratio is respectively 95.2% and 20.7%.The enantiomeric excess value of product reduces when higher or lower than 40 DEG C.Therefore temperature is
It is suitble to full cell to split (±)-methyl phenyl carbinyl acetate at 40 DEG C and obtains (R) -1- benzyl carbinol of high-optical-purity.
4.3 cell concentrations split the influence of (±)-methyl phenyl carbinyl acetate to full cell
It is 8mg/mL (WCW), 16mg/mL (WCW), 24mg/mL (WCW), 32mg/mL (WCW), 40mg/ in cell concentration
ML (WCW), 48mg/mL (WCW), 56mg/mL (WCW), 64mg/mL (WCW), 72mg/mL (WCW), 80mg/mL (WCW)
5mM substrate (±)-acetic acid Soviet Union is separately added into the pH 6.0 of the full cell of Bacillus sp.DL-2,50mM PB reaction system to close
Fragrant ester reacts 4h at 40 DEG C with 200rpm, with gas chromatography chiral post detection and calculates product alcohol enantiomeric excess value and bottom
Object conversion ratio, is as a result shown in Fig. 4.The substrate transformation rate increases with cell concentration and is increased, and product alcohol enantiomeric excess value is first with cell
The increase of concentration and increase, to cell concentration be 32mg/mL (WCW) when reach maximum value, then with cell concentration increase and subtract
It is small, when showing that cell concentration is 32mg/mL (WCW), product alcohol pair best to the fractionation effect of (±)-methyl phenyl carbinyl acetate
It reflects body excessive value and the substrate transformation rate is respectively 95.2% and 25.6%.
4.4 concentration of substrate split the influence of (±)-methyl phenyl carbinyl acetate to full cell
In pH 6.0,50mM the PB reaction for the full cell of Bacillus sp.DL-2 that cell concentration is 32mg/mL (WCW)
Substrate (±)-acetic acid Soviet Union that concentration is 2.5mM, 5mM, 10mM, 15mM, 20mM, 30mM, 40mM, 50mM is separately added into system
Blending ester reacts 4h at 40 DEG C with 200rpm, with gas chromatography chiral post detection and calculates e.e.pAnd C, as a result see Fig. 5.Table
Bright concentration of substrate be 5mM when, it is best to the fractionation effect of (±)-methyl phenyl carbinyl acetate, the enantiomeric excess value of product alcohol and
The substrate transformation rate is respectively 95.3% and 21.4%.E.e. when concentration of substrate is lower than 5mMpValue decline, substrate converts when being higher than 5mM
Rate is substantially reduced.
4.5 reaction time split the influence of (±)-methyl phenyl carbinyl acetate to full cell
The Bacillus that cell concentration is 32mg/mL (WCW) is added in the buffer solution system of pH 6.0 (50mM PB)
The full cell of sp.DL-2 and 5mM substrate (±)-methyl phenyl carbinyl acetate, reacted respectively at 40 DEG C with 200rpm 1h, 2h, 4h, 6h,
Then 8h, 10h, 12h with gas chromatographic detection and calculate product alcohol enantiomeric excess value e.e.pWith the substrate transformation rate C, as a result
See Fig. 6.When showing that the reaction time is 4h, product alcohol enantiomeric excess value is up to 95.4%, and the substrate transformation rate is at this time
21.0%.Cross that short reaction is incomplete, and the substrate transformation rate is low the reaction time, the enantiomeric excess value drop of reaction time too long product
It is low.4.6 metal ions and surfactant split the influence of (±)-methyl phenyl carbinyl acetate to full cell
The Bacillus that cell concentration is 32mg/mL (WCW) is added in the 50mM PB reaction system of pH 6.0
The full cell of sp.DL-2,5mM substrate (±)-methyl phenyl carbinyl acetate, the surface-active of 2mM metal chlorination salt or 0.05% (w/v)
Agent, not plus metal salt or surfactant are control, not react 4h at 40 DEG C, with gas chromatography chiral post detection, according to peak
Areal calculation e.e.pAnd C, it the results are shown in Table 2.
2 metal ion of table and surfactant split the influence of (±)-methyl phenyl carbinyl acetate to full cell
The presence of most metal ions and surfactant reduces entirely carefully compared with the control group as can be seen from Table 2
The stereoselectivity or the substrate transformation rate of born of the same parents, Na+, K+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, CTAB (cetyl trimethyl bromine
Change ammonium) and CMC-Na (sodium carboxymethylcellulose) to obtained (the R) -1- benzyl carbinol of full cell fractionation (±)-methyl phenyl carbinyl acetate
Three-dimensional selection have facilitation slightly, Cu2+Presence make e.e.pIt has been increased to 95.8%, Fe3+With surfactant SDBS
(neopelex) has obviously inhibiting effect to fractionation.
4.7 organic solvents split the influence of (±)-methyl phenyl carbinyl acetate to full cell
Cell concentration be 32mg/mL (WCW) the full cell of Bacillus sp.DL-2 pH 6.0 (50mM PB) it is anti-
It answers and 5mM substrate (±)-methyl phenyl carbinyl acetate and 5% (v/v) organic solvent is added in system, to be control not added with solvent,
200rpm reacts 4h at 40 DEG C, with gas chromatography chiral post detection, according to calculated by peak area e.e.pAnd C, it the results are shown in Table 3.
3 organic solvent of table splits the influence of (±)-methyl phenyl carbinyl acetate to full cell
The presence of most organic solvents reduces the stereoselectivity and substrate conversion of full cell as can be seen from Table 3
Rate.The presence of acetone, ethyl alcohol, methanol and DMF (N,N-dimethylformamide) improves the enantiomer of product to a certain extent
The addition of excessive value, especially DMF make e.e.pIt has been increased to 95.8%.(reaction system are as follows: dense including cell under optimum condition
Degree is the full cell of Bacillus sp.DL-2,5mM substrate (±)-methyl phenyl carbinyl acetate and the volume fraction of 32mg/mL (WCW)
5% DMF, remaining is the sodium phosphate buffer (PB) of pH6.0；Reaction condition are as follows: at 40 DEG C 200rpm react 4h) reaction before
Gas chromatogram is shown in Figure 12, Figure 13 afterwards.(the R) -1- benzyl carbinol (conversion that optical purity is 96% has been prepared under optimum condition
Rate 21%, yield 41%) (see Figure 15).
Embodiment 5: full cell splits (±)-methyl phenyl carbinyl acetate and prepares (S)-methyl phenyl carbinyl acetate
Different cell concentration or concentration of substrate are added in reaction system has large effect to effect is split, this cell is excellent
First hydrolysis (R)-methyl phenyl carbinyl acetate generates (R) -1- benzyl carbinol, and reduces when cell concentration increases to certain value or concentration of substrate
When to certain value (R)-methyl phenyl carbinyl acetate by basic hydrolysis completely, be further added by cell concentration or reduce concentration of substrate and just start water
Solve (S)-methyl phenyl carbinyl acetate.Therefore, to obtain (S)-methyl phenyl carbinyl acetate of high-optical-purity, increase certain cell concentration
Or concentration of substrate is reduced, carry out the optimization of reaction condition.Enantiomeric excess value (the e.e. of substrate is finally obtaineds) up to
99.8% (S)-methyl phenyl carbinyl acetate.
5.1pH splits the influence of (±)-methyl phenyl carbinyl acetate to full cell
Cell is separately added into the sodium phosphate buffer reaction system of the pH 50mM for being 6.0,6.5,7.0,7.5 and 8.0
Concentration is the full cell of Bacillus sp.DL-2 and 2.5mM substrate (±)-methyl phenyl carbinyl acetate of 32mg/mL (WCW), at 40 DEG C
Under 4h reacted with 200rpm, with gas chromatography chiral post detection, according to calculated by peak area substrate ester enantiomeric excess value e.e.sWith
The substrate transformation rate C, is as a result shown in Fig. 7.
Formula 3:
SacAnd RacRespectively indicate the peak area of (S)-methyl phenyl carbinyl acetate He (R)-methyl phenyl carbinyl acetate.
The enantiomeric excess value of substrate first increases with the increase of pH as can be seen from Figure 7, reaches maximum when to pH being 7.5
Value 99.1%, and have preferable conversion ratio 64.7%, when pH continue to increase the substrate transformation rate continue to increase just be unfavorable for splitting it is anti-
It answers.Therefore the PB of pH 7.5 is suitble to full cell fractionation 2.5mM (±)-methyl phenyl carbinyl acetate to obtain (S)-acetic acid Soviet Union of high-optical-purity
Blending ester.
5.2 temperature split the influence of (±)-methyl phenyl carbinyl acetate to full cell
The Bacillus that cell concentration is 32mg/mL (WCW) is added in the 50mM PB reaction system of pH 7.5
The full cell of sp.DL-2 and 2.5mM substrate (±)-methyl phenyl carbinyl acetate, in different temperatures (20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40
DEG C, 45 DEG C, 50 DEG C) under with 200rpm react 4h, with gas chromatography chiral post detection and calculate e.e.sAnd C, as a result see Fig. 8.Knot
Fruit shows to be gradually increased with the raising substrate enantiomer excessive value of reaction temperature, reaches highest when to 40 DEG C, at this time substrate ester
Enantiomeric excess value and the substrate transformation rate be respectively 98.9% and 67.5%, temperature increases e.e. againsJust it is gradually reduced.Therefore it is warm
Degree is suitble to full cell fractionation (±)-methyl phenyl carbinyl acetate to obtain (S)-methyl phenyl carbinyl acetate of high-optical-purity when being 40 DEG C.
5.3 cell concentrations split the influence of (±)-methyl phenyl carbinyl acetate to full cell
It is 8mg/mL (WCW), 16mg/mL that cell concentration is separately added into the 50mM PB reaction system of pH 7.5
(WCW)、24mg/mL(WCW)、32mg/mL(WCW)、40mg/mL(WCW)、48mg/mL(WCW)、56mg/mL(WCW)、64mg/
The full cell of Bacillus sp.DL-2 and 2.5mM substrate (±)-acetic acid of mL (WCW), 72mg/mL (WCW), 80mg/mL (WCW)
Styracin reacts 4h at 40 DEG C with 200rpm, with gas chromatography chiral post detection and calculates e.e.sAnd C, as a result see Fig. 9.
When showing that cell concentration is 32mg/mL (WCW), the mapping of substrate ester best to the fractionation effect of (±)-methyl phenyl carbinyl acetate
Body excessive value and the substrate transformation rate are respectively 99.7% and 61.8%.Cell concentration is too high or too low all to reduce fractionation effect.
5.4 concentration of substrate split the influence of (±)-methyl phenyl carbinyl acetate to full cell
In the pH 7.5 (50mM PB) added with the full cell of Bacillus sp.DL-2 that cell concentration is 32mg/mL (WCW)
Reaction system in be separately added into concentration be 2.5mM, 5mM, 10mM, 15mM, 20mM, 30mM, 40mM, 50mM substrate (±)-
Methyl phenyl carbinyl acetate reacts 4h at 40 DEG C with 200rpm, with gas chromatography chiral post detection and calculates e.e.sAnd C, as a result see
Figure 10.With the increase e.e. of concentration of substratesIt gradually decreases, when showing that concentration of substrate is 2.5mM, to (±)-acetic acid storax
The fractionation effect of ester is best, and the enantiomeric excess value of substrate ester and conversion ratio are respectively 99.2% and 69.0% at this time.
5.5 reaction time split the influence of (±)-methyl phenyl carbinyl acetate to full cell
The Bacillus that cell concentration is 32mg/mL (WCW) is added in the reaction system of pH 7.5 (50mM PB)
The full cell of sp.DL-2 and 2.5mM substrate (±)-methyl phenyl carbinyl acetate, reacted respectively at 40 DEG C with 200rpm 1h, 2h, 4h,
Then 6h, 8h, 10h, 12h with gas chromatographic detection and calculate e.e.sAnd C, the result is shown in Figure 11.When showing that the reaction time is 4h,
Best to the fractionation effect of (±)-methyl phenyl carbinyl acetate, the enantiomeric excess value and conversion ratio of substrate ester are respectively 99.2%
With 66.7%.With the extension of reaction time, substrate e.e.sValue gradually rises, and when 4h reaches maximum, and the time is further added by, substrate
Conversion ratio continues to increase, and is unfavorable for resolution reaction.
5.6 metal ions and surfactant split the influence of (±)-methyl phenyl carbinyl acetate to full cell
The Bacillus that cell concentration is 32mg/mL (WCW) is added in the reaction system of pH 7.5 (50mM PB)
The surface of the full cell of sp.DL-2,2.5mM substrate (±)-methyl phenyl carbinyl acetate and 2mM metal chlorination salt or 0.05% (w/v) are living
Property agent, with not plus metal salt or surfactant are control, 200rpm does not react 4h at 40 DEG C, is examined with gas chromatography chiral column
It surveys, according to calculated by peak area e.e.sAnd C, it the results are shown in Table 4.
4 metal ion of table and surfactant split the influence of (±)-methyl phenyl carbinyl acetate to full cell
From table 4, it can be seen that the presence of most metal ions and surfactant is in certain journey compared with the control group
The stereoselectivity of cell is increased on degree, wherein metal ion K+, Ca2+, Co2+, Mg2+, Mn2+, Zn2+, Al3+, Fe3+The surface and
Activating agent Tween-20, TritonX-100, Span 65, CTAB, CMC-Na obtain full cell fractionation (±)-methyl phenyl carbinyl acetate
The e.e. of (the S)-methyl phenyl carbinyl acetate arrivedsIt is increased to 99% or more, especially Al3+With TritonX-100 to resolution reaction
Stereoselectivity has biggish facilitation e.e.s> 99.5%；Cu2+, Ni2+Have with surfactant SDBS to cell fractionation non-
Normal apparent inhibiting effect.
5.7 organic solvents split the influence of (±)-methyl phenyl carbinyl acetate to full cell
The Bacillus that cell concentration is 32mg/mL (WCW) is added in the reaction system of pH 7.5 (50mM PB)
The full cell of sp.DL-2,2.5mM substrate (±)-methyl phenyl carbinyl acetate and 5% (v/v) organic solvent, to be pair not added with solvent
According to 200rpm reacts 4h at 40 DEG C, with gas chromatography chiral post detection, according to calculated by peak area e.e.sAnd C, it the results are shown in Table
5。
5 organic solvent of table splits the influence of (±)-methyl phenyl carbinyl acetate to full cell
The presence of organic solvent all reduces e.e. substantially as can be seen from Table 5s, the presence of methanol is to fractionation influential effect
Less, DMSO promotes the stereoselectivity that fractionation prepares (S)-methyl phenyl carbinyl acetate.(reaction system are as follows: packet under optimum condition
Include cell concentration be the full cell of Bacillus sp.DL-2 of 32mg/mL (WCW), 2.5mM substrate (±)-methyl phenyl carbinyl acetate and
2mM Al3+, remaining is the sodium phosphate buffer (PB) of pH7.5；Reaction condition are as follows: 40 DEG C of reaction 4h) reaction front and back gas-chromatography
Figure is shown in Figure 12, Figure 14.(S)-methyl phenyl carbinyl acetate (conversion ratio that optical purity is up to 99.8% has been prepared under optimum condition
68%, yield 65%) (see Figure 15).
Sequence table
<110>Chinese Academy of Science Nanhai Ocean Research Institute
<120>application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1450
<212> DNA
<213>bacillus DL-2 (Bacillus sp. DL-2)
<400> 1
tttggtccac cttaggcggc tagctcctta cggttactcc accgacttcg ggtgttacaa 60
actctcgtgg tgtgacgggc ggtgtgtaca aggcccggga acgtattcac cgcggcatgc 120
tgatccgcga ttactagcga ttccagcttc atgtaggcga gttgcagcct acaatccgaa 180
ctgagaatgg ttttatggga ttggcttgac ctcgcggtct tgcagccctt tgtaccatcc 240
attgtagcac gtgtgtagcc caggtcataa ggggcatgat gatttgacgt catccccacc 300
ttcctccggt ttgtcaccgg cagtcacctt agagtgccca actaaatgct ggcaactaag 360
atcaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga gctgacgaca 420
accatgcacc acctgtcact ctgtcccccg aaggggaacg ctctatctct agagttgtca 480
gaggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca catgctccac 540
cgcttgtgcg ggcccccgtc aattcctttg agtttcagtc ttgcgaccgt actccccagg 600
cggagtgctt aatgcgttag ctgcagcact aaagggcgga aaccctctaa cacttagcac 660
tcatcgttta cggcgtggac taccagggta tctaatcctg tttgctcccc acgctttcgc 720
gcctcagcgt cagttacaga ccaaaaagcc gccttcgcca ctggtgttcc tccacatctc 780
tacgcatttc accgctacac gtggaattcc gcttttctct tctgcactca agttccccag 840
tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa gaaaccgcct 900
gcgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct 960
gctggcacgt agttagccgt ggctttctgg ttaggtaccg tcaaggtacg agcagttact 1020
ctcgtacttg ttcttcccta acaacagagt tttacgaccc gaaagccttc atcactcacg 1080
cggcgttgct ccgtcagact ttcgtccatt gcggaagatt ccctactgct gcctcccgta 1140
ggagtctggg ccgtgtctca gtcccagtgt ggccgatcac cctctcaggt cggctatgca 1200
tcgttgcctt ggtgagccgt tacctcacca actagctaat gcaccgcggg cccatctgta 1260
agtgatagcc gaaaccatct ttcaatcatc tcccatgaag gagaagatcc tatccggtat 1320
tagcttcggt ttcccgaagt tatcccagtc ttacaggcag gttgcccacg tgttactcac 1380
ccgtccgccg ctaacgtcat agaagcaagc ttctaatcag tcgctcgact gcatgtatag 1440
cacccgccac 1450
Claims (9)
1. the full cell of bacillus sp.DL-2 answering in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis
With；The bacillus sp.DL-2, deposit number are as follows: GDMCC 1.1556.
2. application according to claim 1, which is characterized in that the bacillus sp.DL-2's is complete thin
Born of the same parents split (±)-methyl phenyl carbinyl acetate in catalysis and obtain the application in (R) -1- benzyl carbinol.
3. application according to claim 2, which is characterized in that the catalysis splits (±)-methyl phenyl carbinyl acetate and obtains
(R) -1- benzyl carbinol is to deposit to carry out at ambient in metal ion, surfactant or organic solvent.
4. application according to claim 3, which is characterized in that the metal ion is Na+、K+、Co2+、Cu2+、Mg2+、
Mn2+Or Ni2+；The surfactant is cetyl trimethylammonium bromide or sodium carboxymethylcellulose；Described is organic molten
Agent is acetone, ethyl alcohol, methanol or N,N-dimethylformamide.
5. application according to claim 3, the catalysis splits (±)-methyl phenyl carbinyl acetate and obtains (R) -1- benzyl carbinol
Reaction system are as follows: bacillus sp.DL-2,5mM of 32mg/mL are calculated as with wet cell weight including cell concentration
The n,N-Dimethylformamide of substrate (±)-methyl phenyl carbinyl acetate and volume fraction 5%, remaining is the sodium phosphate buffer of pH6.0
Liquid；Reaction condition are as follows: 40 DEG C of reaction 4h.
6. application according to claim 1, which is characterized in that the bacillus sp.DL-2's is complete thin
Born of the same parents split (±)-methyl phenyl carbinyl acetate in catalysis and obtain the application in (S)-methyl phenyl carbinyl acetate.
7. application according to claim 6, which is characterized in that the catalysis splits (±)-methyl phenyl carbinyl acetate and obtains
(S)-methyl phenyl carbinyl acetate is to deposit to carry out at ambient in metal ion, surfactant or organic solvent.
8. application according to claim 7, which is characterized in that the metal ion is K+、Ca2+、Co2+、Mg2+、Mn2+、
Zn2+、Al3+Or Fe3+；The surfactant is Tween-20, TritonX-100, Span 65, cetyl trimethyl bromine
Change ammonium or sodium carboxymethylcellulose；The organic solvent is dimethyl sulfoxide.
9. application according to claim 7, the catalysis splits (±)-methyl phenyl carbinyl acetate and obtains the conjunction of (S)-acetic acid Soviet Union
The reaction system of fragrant ester are as follows: including cell concentration with wet cell weight be calculated as 32mg/mL bacillus sp.DL-2,
2.5mM substrate (±)-methyl phenyl carbinyl acetate and 2mM Al3+, remaining is the sodium phosphate buffer of pH7.5；Reaction condition are as follows:
40 DEG C of reaction 4h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910066687.1A CN109797185A (en) | 2019-01-24 | 2019-01-24 | Application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910066687.1A CN109797185A (en) | 2019-01-24 | 2019-01-24 | Application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109797185A true CN109797185A (en) | 2019-05-24 |
Family
ID=66560185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910066687.1A Pending CN109797185A (en) | 2019-01-24 | 2019-01-24 | Application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109797185A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030148481A1 (en) * | 2001-12-19 | 2003-08-07 | Asca Gmbh Angewandte Synthesechemie Adlershof | Method for kinetic resolution of racemates of alcohols having one or several stereogenic centers |
| CN101338287A (en) * | 2008-07-25 | 2009-01-07 | 华东理工大学 | Bacillus subtilis esterase and application thereof for producing 1-menthol |
| CN104962533A (en) * | 2015-06-30 | 2015-10-07 | 中国科学院南海海洋研究所 | Novel esterase, encoding gene and application thereof in splitting (+/-)-1-phenethyl alcohol and (+/-)-styralyl acetate |
-
2019
- 2019-01-24 CN CN201910066687.1A patent/CN109797185A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030148481A1 (en) * | 2001-12-19 | 2003-08-07 | Asca Gmbh Angewandte Synthesechemie Adlershof | Method for kinetic resolution of racemates of alcohols having one or several stereogenic centers |
| CN101338287A (en) * | 2008-07-25 | 2009-01-07 | 华东理工大学 | Bacillus subtilis esterase and application thereof for producing 1-menthol |
| CN104962533A (en) * | 2015-06-30 | 2015-10-07 | 中国科学院南海海洋研究所 | Novel esterase, encoding gene and application thereof in splitting (+/-)-1-phenethyl alcohol and (+/-)-styralyl acetate |
Non-Patent Citations (1)
| Title |
|---|
| 公颜慧: "新颖深海微生物酯酶EstC11的酶学性质及其在手性拆分乙酸苏合香酯中的应用", 《微生物学通报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105671010B (en) | A kind of aldehyde Ketoreductase mutant, gene, engineering bacteria and its application | |
| CN101429514B (en) | Di-carbonyl reduction enzyme, its gene and uses thereof | |
| CN102559520B (en) | Method for preparing (S)-(4-chlorphenyl)-(pyridine-2-yl)-methanol by utilizing microbial catalysis | |
| US10196660B2 (en) | Xylene monooxygenase-producing strain Arthrobacter woluwensis and its application | |
| CN103497911A (en) | Application of Chryseobacterium sp. and carbonyl reductase thereof in production of aprepitant chiral intermediate | |
| CN101717745B (en) | Method for efficiently preparing (S)-styrene glycol from carbonyl reductase recombinant bacterium | |
| CN112143764A (en) | Method for preparing intermediate compound of brivaracetam by using biological enzyme catalysis | |
| CN103421823B (en) | Come from the short-chain dehydrogenase of Lei Fusong Salmonella, encoding gene, carrier, engineering bacteria and application | |
| CN109762832B (en) | Carboxylesterase gene, recombinant plasmid, recombinant engineering bacterium, encoding protein and application | |
| CN101407780B (en) | Method for preparing (R)-styrene glycol by changing coenzyme specificity and stereoselectivity via site-directed mutagenesis | |
| US20230287333A1 (en) | Production method of recombinant escherichia coli and high-purity ursodeoxycholic acid | |
| CN109797185A (en) | Application of the full cell of bacillus DL-2 in catalysis (±)-methyl phenyl carbinyl acetate asymmetric hydrolysis | |
| CN105950595B (en) | (-)-gamma-lactam enzyme, gene, mutant, carrier and its preparation and application | |
| CN106086090B (en) | A kind of method that two-step microbial conversion method prepares R-MA | |
| CN102174422B (en) | Lipase producing strain tolerant to organic solvent as well as genes and applications of lipase | |
| CN105602913B (en) | Recombinate carbonyl reduction enzyme mutant ReCR-Mut, encoding gene, engineering bacteria and application | |
| CN106119272A (en) | A kind of efficient coproduction L phenylglycine and the strategy of gluconic acid | |
| CN110982757B (en) | Enterobacter cloacae ZJPH1903 and application | |
| CN108441433B (en) | Rhodotorula mucilaginosa NQ1 and application thereof in preparation of chiral alcohol | |
| CN101182484B (en) | Viscous Serratieae and acetonic acid produced by biotransformation of DL-lactic acid | |
| JP4551978B1 (en) | Genes involved in the growth promoting function of acetic acid bacteria, acetic acid bacteria bred using the genes, and vinegar producing method using the acetic acid bacteria | |
| CN101555460A (en) | Bacillus licheniformis and application thereof | |
| CN110016444A (en) | Acinetobacter calcoaceticus ZJPH1806 and its application for preparing Miconazole chiral intermediate | |
| CN113249348B (en) | Carbonyl reductase, gene thereof, recombinant expression transformant containing the gene and use thereof | |
| CN110699278A (en) | Application of whole cells of bacillus DL-1 in catalytic resolution of styracin acetate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: No.1119 Haibin Road, Nansha District, Guangzhou City, Guangdong Province Applicant after: SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY, CHINESE ACADEMY OF SCIENCES Address before: 510301 No. 164 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant before: SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY, CHINESE ACADEMY OF SCIENCES |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190524 |