CN109793931A - It is a kind of reduce biological tissue's thickness method and its biological tissue - Google Patents
It is a kind of reduce biological tissue's thickness method and its biological tissue Download PDFInfo
- Publication number
- CN109793931A CN109793931A CN201910196814.XA CN201910196814A CN109793931A CN 109793931 A CN109793931 A CN 109793931A CN 201910196814 A CN201910196814 A CN 201910196814A CN 109793931 A CN109793931 A CN 109793931A
- Authority
- CN
- China
- Prior art keywords
- tissue
- thickness
- biological tissue
- freezing
- reducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a kind of methods for reducing biological tissue's thickness, it is briefly exactly under frozen section mode, the rough surface of biological tissue is sliced, retain ontology, and then achieve the purpose that reduce tissue thickness, it is coated with again with adhesive to biological tissue's rough surface after cutting at this time and forms one layer of adhesive face, the features such as medical bio adhesive promotes cell absorption, water-fast, good biocompatibility can be played, promote endothelialization, due to its high viscosity can also cementation rough surface fracture fiber, so that rough surface is become smooth, reduces thrombus.
Description
Technical field:
The invention belongs to biological organization material field, in particular to a kind of method for reducing biological tissue's thickness, to be applied to people
Work heart valve.
Background technique:
The leaflet original adoption porcine aorta valve of biovalve, but valve, there are the limitation of geometry, bovine pericardium valve goes out
After now, manufacture leaflet that can be more convenient, careful.Valve made of cattle pericardium experienced as the intracardiac implantation material of cardiac surgery operation
The test of long-term clinical application has and is chronically implanted that material is more long, better clinical verification than any one.
Intervention biovalve is the rapid development with Interventional cardiology and the minimally invasive intervention heart valve generated, they are logical
It crosses suture valve is sewn on bracket, then bracket is compressed in conduit, catheterization procedure is applied in realization to be set in valve
It changes.Pipe size is smaller, and it is smaller that influence of the valve art to patient is changed in intervention, more advantageously reduces vascular complication.These are new
The relatively thin valvular tissue of valve devices call is intervened, makes size of the valve compression by conduit, however the thickness of bovine pericardial tissue
Degree limits always selecting for valve.
Pericardium is the thin film wrapped up outside heart, can be divided inside and outside two layers, and outer layer is fiber pericardium, and surface is large stretch of
Fat covering, removing fat after, by the intertextures such as the tough and tensile collagenous fibres of multilayer and elastomer intert it is compound form, internal layer be slurry
Film pericardium, surface is smooth, is wrapped in pericardium slurries.Typically, the bovine pericardial tissue thickness range of acquisition be 0.20mm extremely
0.80mm, and most of material thickness is between 0.35-0.70mm.But for intervention heart valve, only sub-fraction is full
The pericardium of sufficient thickness requirement can be used for preparing leaflet, cause the utilization rate of pericardium lower.
To reduce pericardium thickness, pericardium utilization rate is improved, there are many processing modes, such as handle using dry state, machinery pressure
Contracting, cutting etc. reduce leaflet tissue thickness.U.S. Patent number US 7141064 proposes compression bovine pericardium, reduces its thickness
About 50%, to be used to prepare heart valve leaflet, but compression will lead to the irreversible variation of institutional framework, shape, flexibility.It is public
The number of opening is the patent of 102811681 B of CN, is disclosed using laser or such as mechanical dress of razor or planing device shearing tissue
The removal for directly organizationally carrying out fibrous face material is set, to reduce film thickness and keep fibrous face smooth, but due to pericardium
Fiber is to intert compound weave in, forms the fibre morphology of multi-level different orientation, and this laser or mechanical skiving side
Formula does not consider that caused fiber is loose, be broken and high temperature high fever causes irreversible damage to fiber after cutting, subsequent to choose
Select, use etc. that there are still some problems.
The information disclosed in the background technology section is intended only to increase the understanding to general background of the invention, without
It should be considered as recognizing or implying in any form that the information constitutes existing skill already known to those of ordinary skill in the art
Art.
Summary of the invention:
The purpose of the present invention is to provide a kind of methods for reducing biological tissue's thickness, to overcome above-mentioned in the prior art
Defect.
To achieve the above object, the present invention provides it is a kind of reduce biological tissue's thickness method, according to the following steps into
Row:
A, the biological tissue of certain shapes is put into cryoprotector freezing, forms frozen block；
B, entire frozen block is put into freezing-microtome and carries out photo fix；
C, the frozen block after removing photo fix is thawed, and the tissue of suitable thickness is obtained after cleaning；
D, required tissue is obtained with medical bio adhesive cementation trimming face.
The technical solution that the present invention further limits are as follows:
Preferably, in above-mentioned technical proposal, step A, treated that tissue thickness can reduce by B, C, can also be without any change
Change, the processing without any variation refers to the surface flotation fiber in only trim fiber face or tissue fibers face is made to polish.
Preferably, in above-mentioned technical proposal, biological tissue is that the pericardium of mammal or lactation are moved in step A
The biomembrane at the other positions of object.
Preferably, in above-mentioned technical proposal, tissue can be tissue before crosslinking Treatment or after crosslinking Treatment, at crosslinking
Reason can be using the processing methods such as chemical method or other synthetic cross-linking agents or two methods mixing crosslinking.
Preferably, in above-mentioned technical proposal, chemical method be glutaraldehyde, carbonization imines, oneself different dicyanate, Geniposide,
Anthocyanidin or other synthetic cross-linking agent processing methods, physical method are severe dehydration, ultraviolet light processing method.
Preferably, in above-mentioned technical proposal, freezing is put into biological tissue is smooth in freeze box in the step A, is added
Enter cryoprotector, is freezed at -20 DEG C--80 DEG C.Frozen section is that one kind is cooled fast to tissue under cryogenic
Certain degree of hardness, the method being then sliced, the rapid pathological diagnosis more being applied in operation.Preferably, above-mentioned technical proposal
In, cryoprotector is the mixed liquor of macromolecule polymer solution or several macromolecule polymer solutions in the step A, described
Macromolecule polymer solution is the water-soluble mixing of poly-vinyl alcohol solution, polyvinylpyrrolidone, polyethylene glycol and polyvinyl alcohol
The water soluble mixt of object, polyethylene glycol and polyvinyl alcohol and methylcellulose, B ultrasound couplant, other are colorless or colored
Embed reagent.
Preferably, in above-mentioned technical proposal, freeze box refer to the plastic casing of flexibility or rigidity compatible with tissue profile/
Mold or stainless steel box.
Preferably, in above-mentioned technical proposal, freezing-microtome refers to commercially available all kinds of freezing-microtomes in step B, can
Continuous cutting sheet or small pieces tissue.
Preferably, in above-mentioned technical proposal, photo fix refers to freezing-microtome by the different photo fix thickness pair of setting in step B
The fibrous face of tissue is cut.
Preferably, in above-mentioned technical proposal, tissue is cut to the cutting property of can choose, obtaining has uniform but different-thickness
The tissue in region.
Preferably, in above-mentioned technical proposal, cementation trimming face refers to one or more medical bio adhesives in step D
Pass through the fiber disconnected section of fibrous face exposure after painting type or atomizing or the closing cementation trimming such as instillation or push type.
Preferably, in above-mentioned technical proposal, medical bioadhesive is soft tissue medical adhesive in step D, is alpha-cyano third
The secondary monooctyl ester of olefin(e) acid N-butyl, isobutyl alpha-cyanoacrylate, alpha-cyanoacrylate, medical grade high viscosity sea-mussel mucin and its
One of derivative, imitative mussel viscosity macromolecule.Sea-mussel mucin, also referred to as mussel byssus protein are that seashells purple is made a gift of
Shellfish etc. generates and stores a kind of basic protein glue in special body of gland, rich in 3,4-dihydroxyphenyl-L-alanine (DOPA, DOPA) and
Lysine.Due to its superpower adhesion property, all solids substrate surface can be almost adhered to, in surface chemistry, biology doctor
Etc. fields have a wide range of applications.Sea-mussel mucin adherency does not have toxicity and immunogenicity, binding ability and service life to people
It is not influenced by water.In addition, the presence of lysine makes albumen with high carrying capacity positive charge, and the various cells of human body are negatively charged
Lotus, sea-mussel mucin promote cell adherence, growth by electrostatic interaction and cell combination.
Preferably, in above-mentioned technical proposal, cementation can also be cemented in one or more medical bio adhesives due to
Fiber tearing between tissue and the tissue space being layered.In valve production process, materials, fixation, cutting and seam that early period organizes
The processes such as conjunction will cause the fiber tearing at organization internal, especially cut edge, and then lead to edge organizational hierarchy.Pass through
The tissue of bioadhesive closing layering, to improve output capacity.
A kind of sheet biological tissue that thickness reduces, the biological tissue obtained using any of the above-described the method.
Under frozen section mode, the rough surface of pericardial tissue is sliced, retains pericardium ontology, and then reach reduction
The purpose of tissue thickness is coated with to the pericardium rough surface after cutting again as raw material using sea-mussel mucin forms one layer of adherency at this time
Face can play the features such as sea-mussel mucin promotes cell absorption, water-fast, good biocompatibility, promote endothelialization, due to its height
Viscosity can also cementation rough surface fracture fiber, so that rough surface is become smooth, reduce thrombus.
Compared with prior art, the invention has the following beneficial effects:
(1) lamellar structure is trimmed by the way of freezing cutting, traditional mode cut tissue longitudinal section and retain slice of forgoing,
Precisely quantization cutting directly is carried out to the cross section of tissue and retains tissue matrix, thickness of cutting is more controllable, reaches reduction group
Knit the purpose of thickness, cutting convenience is greatly improved, avoid the direct calcination of laser cause fibrous face surface fiber to burn,
Fibre modification and calcination thickness disadvantage not easy to control, and avoid dermatotome directly planing leads to thickness on tissue fibers surface
Inhomogeneities and to the scuffing of fibrous face, tear, be broken equivalent damage.
(2) cryoprotector selected freezes speed with tissue and soft or hard toughness is close, and when freezing solidifies together, and has
The effects of supporting filling, reducing gauffer and fracture, can fixing organization shape rapidly, protect the knot of tissue to a greater extent
Structure.
(3) the fiber disconnected section generated after cementation cutting is carried out using medical bio adhesive especially sea-mussel mucin,
Guarantee the flexibility of tissue, while improving the utilization rate and durability of tissue, keep fibrous face more smooth, and sea-mussel mucin has
Have and promote the functions such as endothelial cell adherency, leaflet endothelialization can be made, reduce thrombotic risk, improves valve whole service life.
Detailed description of the invention:
Fig. 1 is freezing cutting schematic diagram.
Fig. 2 is the sample schematic diagram of freezing cutting.
Fig. 3 is the schematic diagram of the tissue dropwise addition medical bio adhesive cementation fibrous face after freezing cutting.
The meaning respectively marked in figure are as follows: 1- freezes cutting machine, 2- refrigeration operation room, 3- blade holder, 4- blade, 5- knife
Holder connection device, 6- control tool carrier jointing device handle, 7- freezing tissue block sample, 8- sample stage, 9- Bit andits control dress
It sets, 10- medical bio adhesive packaging bottle, 71- has cut the fibrous face of tissue, the non-cutting tip freezing tissue of 72-, 101-
Medical bio adhesive, tissue fibers face when 711- cementation.
Specific embodiment:
Specific embodiments of the present invention will be described in detail below, it is to be understood that protection scope of the present invention not by
The limitation of specific embodiment.
Unless otherwise explicitly stated, otherwise in entire disclosure and claims, term " includes " or its change
Changing such as "comprising" or " including " etc. will be understood to comprise stated element or component, and not exclude other
Element or other component parts.
Embodiment 1:
Bovine pericardial tissue is obtained in local slaughterhouse, 0.625% glutaraldehyde fixes 3 days after being stripped, trim and cleaning, physiology salt
It is rectangular that the bovine pericardial tissue that average thickness is 0.45mm is cut into 60x60mm after water cleaning, tissue fibers are smooth downwards
It is put into the smooth 80x80x5mm plastic casing in bottom surface, poly-vinyl alcohol solution is added, is totally submerged tissue, then by entire box body one
It rises and is put into -80 DEG C of refrigerator freezing 0.5h.It will freeze in the refrigeration operation room 2 that box body is transferred to rapidly in freezing cutting machine 1, remove group
Block is knitted, a little poly-vinyl alcohol solution is added dropwise in sample stage 8,7 smooth surface of freezing tissue block sample presses rapidly downwards upper poly- second
Enolate solution makes tissue block cryofixation in sample stage 8, the fixed blade 4 in blade holder 3, adjusts displacement control
Device 9 processed makes freezing tissue block sample 7 close to blade 4, sets photo fix with a thickness of 10 μm, rotation control knife rest connects dress manually
Handle 6 is set, by the rotation of tool carrier jointing device 5, the movement of 3 cutter 4 of blade holder is driven, thus to freezing tissue block
Sample 7 carries out the excision of 10 μ m thicks, and multiple rotational handle cuts off the cryoprotector that non-cutting tip freezing tissue 72 has more,
Make the exposure of tissue fibers face, cut the fibrous face 71 of tissue, then continuous 15 rotations control tool carrier jointing device handle
6, the thickness of 150 μm of excision makes bovine pericardium thickness be reduced to 0.30mm.Freezing after removing sample stage 8 and cutting together
Tissue block is placed on and thaws at room temperature, then with physiological saline cleansing tissue, then will tissue smooth surface is smooth downwards is placed on clean bench
On, medical bio adhesive packaging bottle 10 is opened, 101 sea-mussel mucin of medical bio adhesive is added dropwise, makes to cut with stick smearing is applied
Rear fiber cementation is cut, obtains the tissue fibers face 711 when cementation, fibrous face is smooth.
Embodiment 2:
Pig pericardial tissue is obtained in local slaughterhouse, with 1% carbonization imines and 0.625% glutaraldehyde after being stripped, trim and cleaning
3 days or more are fixed, it is 50mm circle that the pig pericardial tissue that average thickness is 0.28mm, which is cut into diameter, after physiological saline cleaning,
By tissue fibers, the smooth smooth diameter in bottom surface that is put into is 60mm, in a height of 3mm round plastic basin downwards, be added polyethylene glycol and
The water soluble mixt of polyvinyl alcohol is totally submerged tissue, then entire box body is put into togerther -20 DEG C in freezing cutting machine 1
In refrigeration operation room 2, tissue block is removed, the water solubility that a little polyethylene glycol and polyvinyl alcohol are added dropwise in sample stage 8 is mixed
Object is closed, 7 smooth surface of freezing tissue block sample presses rapidly downwards the water soluble mixt of upper polyethylene glycol and polyvinyl alcohol, makes tissue block
Cryofixation is in sample stage 8, the fixed blade 4 in blade holder 3, adjusts displacement control device 9, makes freezing tissue
Block sample 7 sets photo fix close to blade 4 with a thickness of 5 μm, rotation control tool carrier jointing device handle 6, is connected by knife rest manually
The rotation of device 5 drives the movement of 3 cutter 4 of blade holder, cuts to carry out 5 μ m thicks to freezing tissue block sample 7
It removes, multiple rotational handle cuts off the cryoprotector that non-cutting tip freezing tissue 72 has more, and makes the exposure of tissue fibers face, obtains
The fibrous face 71 for having cut tissue, is rotated further by 1 secondary control tool carrier jointing device handle 6, and the thickness of 5 μm of excision wipes out pig substantially
The surface of pericardium fibrous face overhangs fiber.Freezing tissue block after removing sample stage 8 and cutting together, is placed on and solves at room temperature
Freeze, then use physiological saline cleansing tissue, then will tissue smooth surface downwards it is smooth be placed on clean bench, open medical bio bond
101 sea-mussel mucin of medical bio adhesive is added dropwise in agent Packaging Bottle 10, makes fiber cementation after cutting with stick smearing is applied, is glued
Gu when tissue fibers face 711, fibrous face is smooth.
Embodiment 3:
Bovine pericardial tissue is obtained in local slaughterhouse, 0.5% oneself different dicyanate fixes 5 days after being stripped, trim and cleaning, raw
It is 100mm circle that the bovine pericardial tissue that average thickness is 0.50mm, which is cut into diameter, after reason salt water cleaning, by tissue fibers face
The smooth smooth diameter in bottom surface that is put into is 110mm, in a height of 3mm circular flexible plastic tub downwards, and the freezing of Neg-50 green is added
Embedding medium is totally submerged tissue, then entire box body is put into togerther in -40 DEG C of refrigeration operation rooms 2 in freezing cutting machine 1, takes
Undertissue's block, is added dropwise a little Neg-50 green frozen embedding agent in sample stage 8, and freezing tissue block 7 smooth surfaces of sample are downward
Upper Neg-50 green frozen embedding agent is pressed rapidly, makes tissue block cryofixation in sample stage 8, in blade holder 3
Fixed blade 4 adjusts displacement control device 9, makes freezing tissue block sample 7 close to blade 4, sets photo fix with a thickness of 10 μm, hand
The dynamic control tool carrier jointing device handle 6 of turn drives 3 cutter 4 of blade holder by the rotation of tool carrier jointing device 5
Mobile, to carry out the excision of 10 μ m thicks to freezing tissue block sample 7, multiple rotational handle cuts off non-cutting tip freezing group
72 cryoprotectors having more are knitted, the exposure of tissue fibers face is made, have been cut the fibrous face 71 of tissue, then continuous 15 rotations
Tool carrier jointing device handle 6 is controlled, 150 μm of half Regional resection of thickness of bovine pericardium circular disc is made.More allowing blade replacement 4, again
Displacement control device 9 is adjusted, makes the other half bovine pericardium region close to blade 4, resets photo fix with a thickness of 20 μm, rotate manually
Tool carrier jointing device handle 6 is controlled, by the rotation of tool carrier jointing device 5, drives the movement of 3 cutter 4 of blade holder, from
And the excision of 20 μ m thicks is carried out to the other half bovine pericardium region, then continuous 9 rotations control tool carrier jointing device handle 6, make
The thickness of 200 μm of bovine pericardium half Regional resection, and then obtaining half area thickness is 0.35mm, the other half area thickness is
The bovine pericardium disk of 0.30mm.Freezing tissue block after removing sample stage 8 and cutting together, is placed on and thaws at room temperature, then
With physiological saline cleansing tissue, then will tissue smooth surface is smooth downwards is placed on clean bench, open medical bio adhesive packet
Bottling 10 makes fiber cementation after cutting using the secondary monooctyl ester of atomizing alpha-cyanoacrylate, obtains tissue fibers face when cementation
711, fibrous face is smooth.
Embodiment 4:
Bovine pericardial tissue is obtained in local slaughterhouse, after being stripped, trim and cleaning, the bovine pericardium for being 0.35mm by average thickness
Tissue is cut into that 80x60mm is rectangular, is put into the smooth 90x80x4mm flexiplast box in bottom surface for tissue fibers are smooth downwards
In, the water soluble mixt of polyethylene glycol and polyvinyl alcohol is added, is totally submerged tissue, then entire box body is put into togerther -20
DEG C refrigerator freezing 1h.It will freeze in the refrigeration operation room 2 that box body is transferred to rapidly in freezing cutting machine 1, tissue block removed, in sample
The water soluble mixt of a little polyethylene glycol and polyvinyl alcohol is added dropwise on objective table 8, freezing tissue block 7 smooth surfaces of sample are rapid downwards
The water soluble mixt of polyethylene glycol, polyvinyl alcohol and polyvinylpyrrolidone in pressure carries tissue block cryofixation in sample
On object platform 8, the fixed blade 4 in blade holder 3 adjusts displacement control device 9, makes freezing tissue block sample 7 close to blade
4, photo fix is set with a thickness of 5 μm, and rotation control tool carrier jointing device handle 6, passes through the rotation of tool carrier jointing device 5, band manually
The movement of 3 cutter 4 of cutting blade fixed frame repeatedly rotates hand to carry out the excision of 5 μ m thicks to freezing tissue block sample 7
Handle cuts off the cryoprotector that non-cutting tip freezing tissue 72 has more, and makes the exposure of tissue fibers face, has been cut tissue
Fibrous face 71, then continuous 14 rotations control tool carrier jointing device handle 6, and the thickness of 70 μm of excision makes the reduction of bovine pericardium thickness
For 0.28mm.Freezing tissue block after removing sample stage 8 and cutting together, is placed on and thaws at room temperature, then use physiological saline
Cleansing tissue, then will organize smooth surface is smooth to be downwards placed on clean bench, medical bio adhesive packaging bottle 10 is opened, is added dropwise
α-cyanoacrylaten-butyl and dopamine mixed solution make fiber cementation after cutting with stick smearing is applied, obtain group when cementation
Textured fiber face 711, fibrous face is smooth.
Above embodiment is can not to be interpreted as in order to illustrate embodiment disclosed by the invention to limit of the invention
System.Although a variety of particular preferred embodiments of the invention has been combined to carry out specific description to the present invention, it is to be understood that, this
Invention should not be limited only to these specific embodiments.In fact, various show for those skilled in the art as described above
And the modification being clear to is intended to be included within the scope of this invention to obtain invention.
The aforementioned description to specific exemplary embodiment of the invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining specific principle of the invention and its actually answering
With so that those skilled in the art can be realized and utilize a variety of different exemplary implementation schemes of the invention and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (15)
1. a kind of method for reducing biological tissue's thickness, which is characterized in that follow the steps below:
A, the biological tissue of certain shapes is put into cryoprotector freezing, forms frozen block；
B, entire frozen block is put into freezing-microtome and carries out photo fix；
C, the frozen block after removing photo fix is thawed, and the tissue of suitable thickness is obtained after cleaning；
D, required tissue is obtained with medical bio adhesive cementation trimming face.
2. the method according to claim 1 for reducing biological tissue's thickness, which is characterized in that step A, treated by B, C
Tissue thickness can reduce, and can also refer to the surface in only trim fiber face without the processing of any variation without any variation
Floating fiber makes tissue fibers face polish.
3. the method according to claim 1 for reducing biological tissue's thickness, which is characterized in that biological group in the step A
It is woven to the pericardium of mammal or the biomembrane at the other positions of mammal.
4. the method according to claim 2 for reducing biological tissue's thickness, which is characterized in that tissue can be crosslinking Treatment
Tissue after preceding or crosslinking Treatment, crosslinking Treatment can use the processing methods such as chemical method or other synthetic cross-linking agents, or
Two methods of the mixing crosslinking of person's.
5. it is according to claim 3 reduce biological tissue's thickness method, which is characterized in that chemical method be glutaraldehyde,
Be carbonized imines, oneself different dicyanate, Geniposide, anthocyanidin or other synthetic cross-linking agent processing methods, and physical method is de- for severe
Water, ultraviolet light processing method.
6. the method according to claim 1 for reducing biological tissue's thickness, which is characterized in that freezing in the step A is
It is put into biological tissue is smooth in freeze box, cryoprotector is added, is freezed at -20 DEG C--80 DEG C.
7. reducing the method for biological tissue's thickness according to claim 1 or 5, which is characterized in that freezed in the step A
Protective agent is the mixed liquor of macromolecule polymer solution or several macromolecule polymer solutions, and the macromolecule polymer solution is
Poly-vinyl alcohol solution, polyvinylpyrrolidone, polyethylene glycol and polyvinyl alcohol water soluble mixt, polyethylene glycol and polyethylene
The water soluble mixt of alcohol and methylcellulose, B ultrasound couplant, other colorless or colored embedding reagents.
8. the method according to claim 5 for reducing biological tissue's thickness, which is characterized in that the freeze box refers to and group
Knit the plastic casing/mold or stainless steel box of the compatible flexibility or rigidity of shape.
9. the method according to claim 1 for reducing biological tissue's thickness, which is characterized in that freeze and cut in the step B
Piece machine refers to commercially available all kinds of freezing-microtomes, can continuously cut large stretch of or small pieces tissue.
10. the method according to claim 1 for reducing biological tissue's thickness, which is characterized in that photo fix is in the step B
Refer to that freezing-microtome cuts the fibrous face of tissue by the different photo fix thickness of setting.
11. the method according to claim 10 for reducing biological tissue's thickness, which is characterized in that the cutting property of can choose
Tissue is cut, the tissue with uniform but different-thickness region is obtained.
12. the method according to claim 1 for reducing biological tissue's thickness, which is characterized in that cementation trims face in step D
Refer to and is repaired with one or more medical bio adhesives by painting type or atomizing or the closing cementation such as instillation or push type
Cut the fiber disconnected section of rear fibrous face exposure.
13. reducing the method for biological tissue's thickness according to claim 1 or described in 12, which is characterized in that medical life in step D
Object adhesive is soft tissue medical adhesive, secondary for α-cyanoacrylaten-butyl, isobutyl alpha-cyanoacrylate, alpha-cyanoacrylate
One of monooctyl ester, the sea-mussel mucin of medical grade high viscosity and its derivative, imitative mussel viscosity macromolecule.
14. the method according to claim 12 for reducing biological tissue's thickness, which is characterized in that cementation can also use one kind
Or a variety of medical bio adhesives cement in the tissue space being layered due to fiber tearing between tissue.
15. the sheet biological tissue that a kind of thickness reduces, which is characterized in that using any claim institute in claim 1-14
State the biological tissue of method acquisition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910196814.XA CN109793931A (en) | 2019-03-15 | 2019-03-15 | It is a kind of reduce biological tissue's thickness method and its biological tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910196814.XA CN109793931A (en) | 2019-03-15 | 2019-03-15 | It is a kind of reduce biological tissue's thickness method and its biological tissue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109793931A true CN109793931A (en) | 2019-05-24 |
Family
ID=66562860
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910196814.XA Pending CN109793931A (en) | 2019-03-15 | 2019-03-15 | It is a kind of reduce biological tissue's thickness method and its biological tissue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109793931A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112206350A (en) * | 2020-09-23 | 2021-01-12 | 张飞 | Pretreatment method of bovine pericardial tissue for manufacturing biological valve |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750922A (en) * | 2013-12-31 | 2014-04-30 | 金仕生物科技（常熟）有限公司 | Artificial heart valve and valve leaflet manufacturing method |
| CN108472140A (en) * | 2016-01-07 | 2018-08-31 | 美敦力瓦斯科尔勒公司 | The reparation and reinforcing of artificial creature's tissue |
-
2019
- 2019-03-15 CN CN201910196814.XA patent/CN109793931A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750922A (en) * | 2013-12-31 | 2014-04-30 | 金仕生物科技（常熟）有限公司 | Artificial heart valve and valve leaflet manufacturing method |
| US10149755B2 (en) * | 2013-12-31 | 2018-12-11 | Kingstronbio (Changshu) Co., Ltd. | Method for preparing an artificial heart valve leaflet |
| CN108472140A (en) * | 2016-01-07 | 2018-08-31 | 美敦力瓦斯科尔勒公司 | The reparation and reinforcing of artificial creature's tissue |
Non-Patent Citations (7)
| Title |
|---|
| 吕申: "《病理检验技术》", 31 August 2014, 人民军医出版社 * |
| 张奇: "《国防特色教材 军用药物制剂工程学》", 30 April 2012, 北京理工大学出版社 * |
| 张德华: "《蛋白质与酶工程》", 30 September 2015, 合肥工业大学出版社 * |
| 来茂德: "《病理学高级教程 精装珍藏本》", 28 February 2015, 人民军医出版社 * |
| 杨力等: "《组织修复生物力学》", 31 December 2017, 上海交通大学出版社 * |
| 杨勇骥: "《医学生物电子显微镜技术》", 31 August 2012, 第二军医大学出版社 * |
| 顾其胜等: "《胶原蛋白与临床医学》", 31 March 2003, 第二军医大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112206350A (en) * | 2020-09-23 | 2021-01-12 | 张飞 | Pretreatment method of bovine pericardial tissue for manufacturing biological valve |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2678945B2 (en) | Artificial blood vessel, method for producing the same, and substrate for artificial blood vessel | |
| US20200360039A1 (en) | Method and apparatus for tissue grafting and copying | |
| US10653520B2 (en) | Method of preparing a tissue swatch for a bioprosthetic device | |
| US10149755B2 (en) | Method for preparing an artificial heart valve leaflet | |
| CA1335432C (en) | Tissue graft composition and method | |
| Graham et al. | Endothelial cell seeding of prosthetic vascular grafts: early experimental studies with cultured autologous canine endothelium | |
| US6352708B1 (en) | Solution and method for treating autologous tissue for implant operation | |
| US4956178A (en) | Tissue graft composition | |
| GB1602906A (en) | Heart valve prosthesis and method for the production thereof | |
| Simionescu et al. | Mapping of glutaraldehyde‐treated bovine pericardium and tissue selection for bioprosthetic heart valves | |
| CA2493535A1 (en) | Valved prostheses with preformed tissue leaflets | |
| AU2016324162B2 (en) | Compositions derived from placenta and methods of producing the same | |
| JP2017512544A (en) | Membrane support and package | |
| JPWO2004073761A1 (en) | Regenerated corneal endothelial cell sheet, production method and use thereof | |
| CN109793931A (en) | It is a kind of reduce biological tissue's thickness method and its biological tissue | |
| CN106563173B (en) | Acellular biological dermis material and preparation method and application thereof | |
| CN103961752A (en) | Guided tissue regeneration membrane and preparation method thereof | |
| Maeda et al. | Thoracoscopic cell sheet transplantation with a novel device | |
| CN201333112Y (en) | Non-support heterologous bioprosthetic heart valve | |
| CN1117586C (en) | Process for preparing biological material as artificial heart valve | |
| Ichikawa et al. | A new antithrombogenic RV-PA valved conduit | |
| RU2797632C1 (en) | Method of manufacturing a vascular graft from the vein of the human umbilical cord | |
| CN109363801A (en) | A kind of method that femtosecond laser technology auxiliary prepares porcine cornea endothelium plant piece | |
| CN117085183B (en) | In-situ curing and seamless transplanting material and preparation method and application thereof | |
| CN106491237A (en) | Compound cross linked porous breast sticking patch and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190524 |
|
| RJ01 | Rejection of invention patent application after publication |