CN1117586C - Process for preparing biological material as artificial heart valve - Google Patents
Process for preparing biological material as artificial heart valve Download PDFInfo
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- CN1117586C CN1117586C CN 99112286 CN99112286A CN1117586C CN 1117586 C CN1117586 C CN 1117586C CN 99112286 CN99112286 CN 99112286 CN 99112286 A CN99112286 A CN 99112286A CN 1117586 C CN1117586 C CN 1117586C
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- bovine pericardium
- tanning
- retanning
- artificial heart
- biological material
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Abstract
The present invention provides the technical scheme of a process for preparing biological materials as artificial heart valves, which is characterized in that a calf pericardium is taken; under the state of freshness, fat and connective tissues on the surface of the calf pericardium are removed; firstly, the calf pericardium is pre-tanned in glutaraldehyde solution, is curried and re-tanned for the first time in cationic oil, curried and re-tanned in Cr2O3 solution, immersed into aseptic medicinal glycerine and curried for the second time to complete the tanning operation of the calf pericardium simultaneously; the tanned calf pericardium can be stored in glycerine for a long period of time; after being processed into the finished product of a biological valve, the calf pericardium can be stored in alcohol solution for standby application.
Description
What the present invention relates to is in the artificial method of heart material medically, especially a kind of processing method of biological material as artificial heart valve.
Cardiac valve prosthesis is applied to clinical in nearly 40 years, and clinical confirmation though present mechanical valve prosthesis is durable, needs lifelong anticoagulant, thromboembolism incidence rate height, and shortcoming such as dysfunction easily takes place, still can not solve so far.Biovalve has caused the short defective of life-span of biovalve because reason such as the tanning agent of calcification, degeneration, valve designs and biomaterial is undesirable.In the prior art, the biovalve of normal application is the bovine pericardium that adopts glutaraldehyde and chromic oxide tanning, and the problem that this biovalve exists is that biovalve is easy to generate calcification and phenomenon of rupture occurs.
Purpose of the present invention is exactly at the existing in prior technology deficiency, and provides a kind of processing method technical scheme of biological material as artificial heart valve, this scheme to adopt twice stuffing to handle, and the flexibility of biovalve is strengthened, and can prolong its service life.
This programme is realized by following technical measures.Mainly be the biovalve material that adopts bovine pericardium to form through glutaraldehyde tanning, its concrete grammar is:
A. get the bovine pericardium of biennial calf, under fresh state in 12 hours, remove the fat and the connective tissue on bovine pericardium surface clean, after the normal saline rinsing, wipe away dried with soft gauze, under the room temperature resting state, the immersion pH value is that 4.5-5.0, concentration are in the glutaraldehyde solution of 0.25-0.35% pre-tanning 45-50 hour;
B. after the bovine pericardium of pretan being finished uses the normal saline rinsing clean, immerse the 1.0-2.0% cation oil at normal temperatures and carried out the stuffing retanning first time in interior 45-50 hour;
C. with the bovine pericardium after the stuffing retanning with the normal saline rinsing clean after, immerse pH value at normal temperatures and be 3.5-4.5, concentration and be in the chromic oxide solution of 0.1-0.2% and to carry out the chromatize retanning in 45-50 hour;
D. with the bovine pericardium after the chromatize retanning with the normal saline rinsing clean after, immerse at normal temperatures and carry out the stuffing second time in the medicinal sterile glycerol, finished the tanning work of bovine pericardium simultaneously, but the good bovine pericardium long-term storage of tanning is in glycerol;
E. the bovine pericardium that tanning is finished, after being processed into the refined biometric lobe, immerse concentration and be preserve in 75% the alcoholic solution stand-by.
According to as can be known to the narration of such scheme, owing in this scheme, at first adopt glutaraldehyde pretan, mainly to be glutaraldehyde combine with amino in the bovine pericardium this process, impel the tropocollagen molecule interlinkage, thereby its immunogenicity is reduced, stability and durability increase, but the life-span that the easy calcification of bovine pericardium of this moment can't improve biovalve.Carrying out the stuffing retanning first time through cation oil again, because of cation oil is nontoxic, is the quaternary ammonium compound that fatty acid and amine form, and for a kind of cationic emulsifier does not react with calcium salt and magnesium salt, therefore has the effect of calcification.Oils and fats is filled out between the bovine pericardium construction unit after the stuffing, has improved the fastness of bovine pericardium, has played the quick bonded effect of prevention chromium and collagen fiber surface molecular simultaneously.The 3rd step was to adopt chromic oxide to carry out retanning, made chromium and carboxyl be combined with the effect of calcification, made bovine pericardium more firm, but handled badly, made chromium and quick combination of bovine pericardium collagen fiber molecular surface, made bovine pericardium become crisp and hard easily.Because have stuffing for the first time to handle, cation oil forms thin film at molecular surface, has hindered chromaking speed, makes chromium be entered intramolecule lentamente, has guaranteed that carboxyl combines more uniformly with chromium.But chromium combines tempering easily with carboxyl, and the bovine pericardium after the chromium retanning is also more crisp and hard, therefore, also needs to carry out the stuffing second time.Glycerol is adopted in stuffing for the second time, and the water absorption of glycerol is strong, can increase the metabolism of bovine pericardium, can protect the cell on original bovine pericardium surface, can make pericardium cover the bovine pericardium surface again behind implant into body, has prevented calcification.Strengthen through the bovine pericardium softness of glycerol retanning, anti-tortuous performance, prolonged the service life of biovalve.This shows that the present invention compared with prior art has outstanding substantive distinguishing features and obvious improvement, its implementation result also is conspicuous.
For clearly demonstrating the technical characterstic of this programme,, this programme is set forth below by a specific embodiment.
Mainly be the biological material of Cardiac valve prosthesis that adopts bovine pericardium to form through glutaraldehyde tanning, its concrete grammar is:
A. get the bovine pericardium of biennial calf, under fresh state in 12 hours, remove the fat and the connective tissue on bovine pericardium surface clean, after the normal saline rinsing, wipe away dried with soft gauze, under the room temperature resting state, immerse pH value and be 4.5, concentration is pre-tanning 48 hours in 0.30% the glutaraldehyde solution;
B. after the bovine pericardium of pretan being finished uses the normal saline rinsing clean, immerse 1.0% cation oil at normal temperatures and carried out the stuffing retanning first time in interior 48 hours;
C. with the bovine pericardium after the stuffing retanning with the normal saline rinsing clean after, immerse pH value at normal temperatures and be 4.0, concentration is to carry out the chromatize retanning in 0.1% the chromic oxide solution in 48 hours;
D. with the bovine pericardium after the chromatize retanning with the normal saline rinsing clean after, immerse at normal temperatures and carry out the stuffing second time in the medicinal sterile glycerol, finished the tanning work of bovine pericardium simultaneously, but the good bovine pericardium long-term storage of tanning is in glycerol;
E. the bovine pericardium that tanning is finished, after being processed into the refined biometric lobe, immerse concentration and be preserve in 75% the alcoholic solution stand-by.
With the biovalve of prior art tanning and the biovalve comparison clinically of this programme method tanning:
The village * *: man, 57 years old, replaced the Bicuspid valve made from the bovine pericardium of glutaraldehyde tanning merely because of the two pathological changes of Bicuspid valve in 1988, serious because of calcification, the lobe leaf is torn, and performs the operation once more in December, 98 and replaces with mechanical valve prosthesis.
Liu * *: woman, 34 years old, replaced the Bicuspid valve made from the bovine pericardium of the inventive method tanning because of the two pathological changes of Bicuspid valve in 1989.After people's endocardium transition hypertrophy due to illness, a leaf biovalve is fixed, caused valvular insufficiency, the discovery of performing the operation once more in January, 99, the lobe leaf made from artificial biomaterial does not have yet not damaged of calcification.
Claims (4)
1. the processing method of a biological material as artificial heart valve mainly is to adopt bovine pericardium to form through glutaraldehyde tanning, and its concrete grammar is:
A. get the bovine pericardium of biennial calf, under fresh state in 12 hours, remove the fat and the connective tissue on bovine pericardium surface clean, after the normal saline rinsing, wipe away dried with soft gauze, under the room temperature resting state, the immersion pH value is that 4.5-5.0, concentration are in the glutaraldehyde solution of 0.25-0.35% pre-tanning 45-50 hour;
B. after the bovine pericardium of pretan being finished uses the normal saline rinsing clean, immerse the 1.0-2.0% cation oil at normal temperatures and carried out the stuffing retanning first time in interior 45-50 hour;
C. with the bovine pericardium after the stuffing retanning with the normal saline rinsing clean after, immerse pH value at normal temperatures and be 3.5-4.5, concentration and be in the chromic oxide solution of 0.1-0.2% and to carry out the chromatize retanning in 45-50 hour;
D. with the bovine pericardium after the chromatize retanning with the normal saline rinsing clean after, immerse at normal temperatures and carry out the stuffing second time in the medicinal sterile glycerol, finished the tanning work of bovine pericardium simultaneously, but the good bovine pericardium long-term storage of tanning is in glycerol;
E. the bovine pericardium that tanning is finished, after being processed into the refined biometric lobe, immerse concentration and be preserve in 75% the alcoholic solution stand-by.
2. the processing method of biological material as artificial heart valve according to claim 1 is characterized in that its optium concentration of described glutaraldehyde solution is 0.3%.
3. the processing method of biological material as artificial heart valve according to claim 1, the optium concentration that it is characterized in that described cation oil solution is 1.0%.
4. the processing method of biological material as artificial heart valve according to claim 1, the optium concentration that it is characterized in that described chromic oxide solution is 0.1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99112286 CN1117586C (en) | 1999-06-28 | 1999-06-28 | Process for preparing biological material as artificial heart valve |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99112286 CN1117586C (en) | 1999-06-28 | 1999-06-28 | Process for preparing biological material as artificial heart valve |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1279114A CN1279114A (en) | 2001-01-10 |
| CN1117586C true CN1117586C (en) | 2003-08-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99112286 Expired - Fee Related CN1117586C (en) | 1999-06-28 | 1999-06-28 | Process for preparing biological material as artificial heart valve |
Country Status (1)
| Country | Link |
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| CN (1) | CN1117586C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2666485C (en) * | 2006-10-27 | 2015-10-06 | Edwards Lifesciences Corporation | Biological tissue for surgical implantation |
| US7651387B2 (en) | 2008-03-28 | 2010-01-26 | Southern Lights Ventures 2002 Limited | Apparatus and method for processing bovine pericardium |
| CN101721745B (en) * | 2009-11-09 | 2015-04-01 | 山东省千佛山医院 | Method for processing donkey pericardium for artificial heart valve and other biological repairing material |
| CN104436339A (en) * | 2013-09-25 | 2015-03-25 | 李温斌 | No-power-property artificial ventricle device |
| WO2015143611A1 (en) * | 2014-03-24 | 2015-10-01 | 金仕生物科技（常熟）有限公司 | Biological material treating method, and biological material treated by using same |
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1999
- 1999-06-28 CN CN 99112286 patent/CN1117586C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1279114A (en) | 2001-01-10 |
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