CN109781689A - A kind of fluorescent biochip detector - Google Patents

A kind of fluorescent biochip detector Download PDF

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Publication number
CN109781689A
CN109781689A CN201910102573.8A CN201910102573A CN109781689A CN 109781689 A CN109781689 A CN 109781689A CN 201910102573 A CN201910102573 A CN 201910102573A CN 109781689 A CN109781689 A CN 109781689A
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CN
China
Prior art keywords
loading
outer housing
fluorescent biochip
platform
light source
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Application number
CN201910102573.8A
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Chinese (zh)
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CN109781689B (en
Inventor
苏秀榕
周君
李妍妍
芦晨阳
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Zhejiang Zhenghegu Biotechnology Co ltd
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Ningbo University
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Priority to CN201910102573.8A priority Critical patent/CN109781689B/en
Priority claimed from CN201910102573.8A external-priority patent/CN109781689B/en
Publication of CN109781689A publication Critical patent/CN109781689A/en
Application granted granted Critical
Publication of CN109781689B publication Critical patent/CN109781689B/en
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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention discloses a kind of fluorescent biochip detectors, feature be include outer housing, fluorescent biochip loader mechanism and image capturing mechanism are provided in outer housing, fluorescent biochip loader mechanism includes a loading pedestal, loading pedestal can be arranged in outer housing to horizontal fore-aft travel, it loads horizontal to control on pedestal and is actively provided with loading bracket, load the loading platform that can be provided with up and downly vertically on bracket for putting fluorescent biochip, it is provided on outer housing and protrudes into mouth for protruding into outer housing and setting aside to for fluorescent biochip on loading platform, image capturing mechanism is arranged on loading platform, it is additionally provided in outer housing for providing the LED light source mechanism of lighting source to image capturing mechanism.Advantage is that 12V power supply power supply can be used in LED light source mechanism, so that the fluorescent biochip detector is suitable for using outdoors, can also be adapted to vehicle-mounted boat-carrying and use, good portability, to effectively increase detection efficiency.

Description

A kind of fluorescent biochip detector
Technical field
The present invention relates to a kind of biochip supplies analysis, more particularly, to a kind of fluorescent biochip detector.
Background technique
Biochip is according to the principle specifically to interact between biomolecule, by biochemical analysis process integration in chip list The high throughput quickly detection to biotic component is realized by marking the signaling molecule in sample to be tested in face.Fluorescence signal point Son is sensitivity highest, the mark molecule for being particularly suitable for highdensity micro-array biochip.Currently, the inspection of such biochip Measurement equipment is usually laser scanner, and this kind of equipment is designed based on laser co-focusing principle, using laser, photomultiplier transit Pipe and lens group provide excitation light source, by stepper motor driving precise guide rail realize progressive scan after, by combination of software by Row scanning image and after signal amplifies, form final detection image.The common feature of the quasi-instrument is that price is high It is expensive, volume is big and heavy, needs the exchange electric drive of 220V, when operation, need stable use environment, need to preheat before use Deng, cannot directly outdoors scene use, it is necessary to outdoors after field sampling, take back have laser scanner laboratory carry out Detection, causes detection efficiency low.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of structures it is simple, small in size, at low cost and be suitable for outdoors Scene directly carries out detection using the fluorescent biochip detector to improve detection efficiency.
The technical scheme of the invention to solve the technical problem is:
A kind of fluorescent biochip detector, including outer housing are provided with fluorescent biochip loading machine in the outer housing Structure and image capturing mechanism, the fluorescent biochip loader mechanism include a loading pedestal, and the loading pedestal can water It is arranged in the outer housing to flat fore-aft travel, horizontal can be controlled on the loading pedestal and actively be provided with loading branch Frame can be provided with the loading platform for putting fluorescent biochip on the loading bracket up and downly vertically, described Outer housing on be provided with for being protruded into the outer housing for the fluorescent biochip and setting aside to the loading Mouth is protruded on platform, the image capturing mechanism is arranged on the loading platform, also sets in the outer housing It is equipped with for providing the LED light source mechanism of lighting source to the image capturing mechanism.
The front and back for driving the loading pedestal to move forward and backward is provided between loading pedestal and the outer housing Linear moving mechanism, the tandem mobile mechanism include before the back-and-forth motion direction setting of the loading pedestal Adjusting screw rod afterwards, the front and back adjusting screw rod are screwed on the loading pedestal, one end of the front and back adjusting screw rod It coaxially is fixedly installed front and back adjusting knob, the outside of the outer housing is arranged in the front and back adjusting knob.Above-mentioned band The tandem mobile mechanism structure that dynamic loading pedestal level moves forward and backward is simple, easy to assembly, at low cost, and mobile stabilization, It is easy to operate using manually adjusting, and it is substantially better than from the lightweight of whole instrument consideration the instrument of motorized adjustment, convenient for taking Band.
It is provided between the loading bracket and the loading pedestal and drives the loading bracket side-to-side movement Left and right linear moving mechanism, the direct mobile mechanism in the left and right include being arranged along the direction that moves left and right of the loading bracket Left and right adjusting screw rod, the left and right adjusting screw rod is screwed on the loading bracket, the left and right adjusting screw rod One end is coaxially fixed with left and right adjusting knob, and the outside of the outer housing is arranged in the left and right adjusting knob.Above-mentioned band The dynamic left and right linear moving mechanism structure for loading support level side-to-side movement is simple, easy to assembly, at low cost, and mobile stabilization, It is easy to operate using manually adjusting, and it is substantially better than from the lightweight of whole instrument consideration the instrument of motorized adjustment, convenient for taking Band.
What the front end face of the loading bracket penetrated through before and after being provided with one slides chamber, the rear end face of the loading platform It is provided with one and the sliding block for sliding chamber and matching, the sliding block, which can slide up and down to be arranged in, described slides chamber It is interior, it is provided with the straight line up and down for driving the loading platform to move up and down between the sliding block and the loading bracket and moves Motivation structure, the linear moving mechanism up and down include with left and right adjusting screw rod adjusting shaft disposed in parallel, it is described Adjusting shaft be located on the left and right adjusting screw rod and be erected on the loading bracket, in the adjusting shaft It coaxially is fixedly installed an adjustment gear, one is provided on the rear end face of the sliding block and is meshed with the adjustment gear tooth Item, one end of the adjusting shaft are coaxially fixedly installed a up and down adjustment knob, and the up and down adjustment knob setting exists The outside of the outer housing.The structure of linear moving mechanism up and down that above-mentioned drive loading platform moves up and down vertically is simple, dress It is at low cost with convenient, and it is mobile stablize, drive adjustment gear to rotate by adjusting shaft rotation, then drive and nibble therewith The rack gear of conjunction moves up and down, thus drive the sliding block being attached thereto slide it is intracavitary slide up and down, realize loading platform up and down Movement;It is easy to operate using manually adjusting, and it is substantially better than from the lightweight of whole instrument consideration the instrument of motorized adjustment, It is easy to carry.
A mounting platform is provided in the outer housing, the mounting platform is located at the fluorescent biochip and fills On mounted mechanism, hollow installation room is provided on the mounting platform, the LED light source mechanism includes LED light source, institute The rear end of the described installation room is arranged in the LED light source stated and the LED light source is to front irradiation, the image capturing mechanism For camera, the camera is vertically provided on the installation room and protrudes into the installation room, the installation room On be provided with biological specimen information amplification on the fluorescent biochip for that will be seated on the loading platform Image enlarger, the image enlarger is arranged between the camera and the loading platform, described Installation interior be provided with the light for emitting the LED light source be projected to the image enlarger and The light projection system of the camera.Image capturing mechanism is camera, and structure is simple, easy to operate, is put by image Great institutions amplify the biological specimen information on fluorescent biochip, then capture the biological specimen information, LED by camera Light source is used to provide light source to the shooting of camera and image enlarger, to obtain relatively clear biological sample image.
The image enlarger is an eyepiece, and the eyepiece is mounted vertically on the installation room and described Eyepiece is co-axially located at the lower section of the camera, and the lower end of the eyepiece is stretched out the installation room and is located at described On loading platform.Image enlarger is an eyepiece, and structure is simple, easy to operate, and can steadily realize biological sample The amplification of information.
The light projection system includes convex lens, light transmitting sheet, refracting plates, the first optical filter and the second optical filter, institute The front of the LED light source is arranged in the convex lens stated, and the front of the convex lens is arranged in the light transmitting sheet, described Refracting plates the front of the light transmitting sheet is set, the refracting plates are obliquely installed upwards from front to back, the LED light The central light beam in source, the center of the convex lens, the center of the light transmitting sheet, the refracting plates center be in it is same On horizontal optical axis, first optical filter and second optical filter are between the upper and lower every setting and for positioned at the camera Between the eyepiece, the refracting plates are described between first optical filter and second optical filter The center of refracting plates, the center of first optical filter and second optical filter center be in same vertical optical axis On, the vertical optical axis and the eyepiece are coaxial.Above-mentioned light projection system structure is simple, and convex lens is used for LED light The light beam that source issues concentrates on horizontal optical axis, is then transmitted away by light transmitting sheet, by the refraction of refracting plates, by light point It does not project on camera and eyepiece, while being filtered out by the setting of the first optical filter and the second optical filter and not needed using arriving Light wave band so that camera can obtain clear reliable biological specimen information pattern, it is ensured that the accuracy of detection.
Control circuit, the camera, the LED light source and the control electricity are provided in the outer housing Road electrical connection, is provided with Datalink Interface and electric power connection interface on the outer housing, the Datalink Interface and The electric power connection interface is electrically connected with the control circuit.Control circuit is used to control shooting, the LED light source of camera Light, the transmission of data and the supply of power supply, be conventional control circuit, electric power connection interface is for external power supply to control Circuit power supply, Datalink Interface are used to connect external display and show the image absorbed by camera, it can also be used to intake figure The data of picture are transmitted.
The display shown for image is connected on the Datalink Interface.Display is for showing by camera The image of intake.
It is provided with placing platform on the loading platform, is provided in the placing platform for by the fluorescence Biochip imports the importing slot being plugged into the placing platform.By said structure design, so that biological core Piece can be easily placed on loading platform.
Compared with the prior art, the advantages of the present invention are as follows: the laser of traditional structure is replaced by LED light source mechanism Device, in conjunction with image capturing mechanism carry out it is Polaroid, fluorescent biochip loader mechanism can front and rear, left and right, it is up and down, be convenient for The viewing area for adjusting fluorescent biochip, to obtain accurate sample information image;12V electricity can be used in LED light source mechanism Source power supply can also be adapted to vehicle-mounted boat-carrying and use so that the fluorescent biochip detector is suitable for using outdoors, structure letter Single, small in size, at low cost and good portability can effectively improve detection efficiency.
Detailed description of the invention
Fig. 1 is the overall structure diagram of fluorescent biochip in inserting of the invention;
Fig. 2 is schematic cross-sectional view of the invention;
Fig. 3 is the forward sight schematic perspective view of fluorescent biochip loader mechanism in the present invention;
Fig. 4 is the rear view three-dimensional structure of fluorescent biochip loader mechanism in the present invention.
Specific embodiment
The present invention will be described in further detail below with reference to the embodiments of the drawings.
As shown in Figures 1 to 4, a kind of fluorescent biochip detector, including outer housing 1 are provided with fluorescence in outer housing 1 Biochip loader mechanism and image capturing mechanism, fluorescent biochip loader mechanism include a loading pedestal 2, load pedestal 2 It can be arranged in outer housing 1 to horizontal fore-aft travel, horizontal can be controlled on loading pedestal 2 and actively be provided with loading bracket 21, Loading platform 22 for putting fluorescent biochip 3 can be provided with up and downly vertically by loading on bracket 21, on outer housing 1 It is provided with and protrudes into mouth 11, image capture for protruding into outer housing 1 and setting aside to for fluorescent biochip 3 on loading platform 22 Mechanism is arranged on loading platform 22, is additionally provided in outer housing 1 for providing the LED of lighting source to image capturing mechanism Light source mechanism.
In this particular embodiment, it loads to be provided with to drive between pedestal 2 and outer housing 1 and loads what pedestal 2 moved forward and backward Tandem mobile mechanism, tandem mobile mechanism include adjusting along the front and back for being moved forward and backward direction setting for loading pedestal 2 Screw rod 201, front and back adjusting screw rod 201, which is screwed onto, to be loaded on pedestal 2, and one end of front and back adjusting screw rod 201 is coaxially fixedly installed The outside of outer housing 1 is arranged in front and back adjusting knob 202, front and back adjusting knob 202.
In this particular embodiment, it loads bracket 21 and loads and be provided with the drive loading fortune of bracket 21 or so between pedestal 2 Dynamic left and right linear moving mechanism, the direct mobile mechanism in left and right include along the left and right for moving left and right direction setting for loading bracket 21 Adjusting screw rod 203, left and right adjusting screw rod 203, which is screwed onto, to be loaded on bracket 21, and one end of left and right adjusting screw rod 203 is coaxially fixed with The outside of outer housing 1 is arranged in left and right adjusting knob 204, left and right adjusting knob 204.
In this particular embodiment, what the front end face of loading bracket 21 penetrated through before and after being provided with one slides chamber 211, loads flat The rear end face of platform 22 be provided with one with the sliding block 221 that slides chamber 211 and match, sliding block 221 can slide up and down to setting and slide In chamber 211, the linear movement machine up and down for driving loading platform 22 to move up and down is provided between sliding block 221 and loading bracket 21 Structure, upper and lower linear moving mechanism include being located at the adjusting shaft 205 disposed in parallel of left and right adjusting screw rod 203, adjusting shaft 205 It on left and right adjusting screw rod 203 and is erected on loading bracket 21, adjusts and be coaxially fixedly installed an adjustment gear in shaft 205 206, it is provided with one on the rear end face of sliding block 221 and is meshed with adjustment gear 206 rack gear 207, the one end for adjusting shaft 205 is coaxial It is fixedly installed a up and down adjustment knob 208, the outside of outer housing 1 is arranged in up and down adjustment knob 208.
In this particular embodiment, a mounting platform 12 is provided in outer housing 1, mounting platform 12 is located at biological core On piece loader mechanism, hollow installation room 13 is provided on mounting platform 12, LED light source mechanism includes LED light source 4, LED light The rear end of installation room 13 is arranged in source 4 and LED light source 4 is to front irradiation, and image capturing mechanism is camera 5, and camera 5 is vertically set Installation room 13 is set on installation room 13 and protruded into, it is raw that the fluorescence being provided on room 13 for that will be seated on loading platform 22 is installed The image enlarger of biological specimen information amplification on object chip 3, image enlarger are arranged in camera 5 and loading platform Between 22, the light being provided in room 13 for emitting LED light source 4 is installed and is projected to image enlarger and camera shooting First 5 light projection system.
In this particular embodiment, image enlarger is an eyepiece 6, and eyepiece 6 is mounted vertically on installation room 13 and mesh Mirror 6 is co-axially located at the lower section of camera 5, and the lower end of eyepiece 6 is stretched out installation room 13 and is located on loading platform 22.
In this particular embodiment, light projection system includes convex lens 101, light transmitting sheet 102, the filter of refracting plates 103, first The front of LED light source 4 is arranged in mating plate 104 and the second optical filter 105, convex lens 101, and light transmitting sheet 102 is arranged in convex lens 101 Front, the front of light transmitting sheet 102 is arranged in refracting plates 103, and refracting plates 103 are obliquely installed upwards from front to back, LED light source 4 Central light beam, the center of convex lens 101, the center of light transmitting sheet 102, refracting plates 103 center be on same level optical axis, First optical filter 104 and the second optical filter 105 every setting and are between the upper and lower the refracting plates 103 between camera 5 and eyepiece 6 Between the first optical filter 104 and the second optical filter 105, the center of refracting plates 103, the center of the first optical filter 104 and The center of two optical filters 105 is on same vertical optical axis, and vertical optical axis and eyepiece 6 are coaxial.
In this particular embodiment, control circuit (not shown), camera 5, LED light source 4 are provided in outer housing 1 It is electrically connected with control circuit, Datalink Interface 15 and electric power connection interface 16, Datalink Interface 15 is provided on outer housing 1 It is electrically connected with electric power connection interface 16 with control circuit.
In this particular embodiment, the display shown for image is connected on Datalink Interface 15 (not show in figure Show).
In this particular embodiment, it is provided with placing platform 2201 on loading platform 22, is provided in placing platform 2201 For fluorescent biochip 3 to be imported the importing slot 2202 being plugged into placing platform 2201.

Claims (10)

1. a kind of fluorescent biochip detector, it is characterised in that including outer housing, it is raw that fluorescence is provided in the outer housing Object chip loader mechanism and image capturing mechanism, the fluorescent biochip loader mechanism includes a loading pedestal, described Loading pedestal can be arranged in the outer housing to horizontal fore-aft travel, horizontal can control actively on the loading pedestal It is provided with loading bracket, the dress for putting fluorescent biochip can be provided on the loading bracket up and downly vertically Carrying platform is provided on the outer housing for protruding into the outer housing and setting aside to for the fluorescent biochip Mouth is protruded on the loading platform, the image capturing mechanism is arranged on the loading platform, and described is outer It is additionally provided in shell for providing the LED light source mechanism of lighting source to the image capturing mechanism.
2. a kind of fluorescent biochip detector as described in claim 1, it is characterised in that the loading pedestal with it is described The tandem mobile mechanism for driving the loading pedestal to move forward and backward is provided between outer housing, the tandem moves Motivation structure includes the front and back adjusting screw rod for being moved forward and backward direction setting along the loading pedestal, the front and back adjusting screw rod It is screwed on the loading pedestal, one end of the front and back adjusting screw rod is coaxially fixedly installed front and back adjusting knob, institute The outside of the outer housing is arranged in the front and back adjusting knob stated.
3. a kind of fluorescent biochip detector as claimed in claim 1 or 2, it is characterised in that the loading bracket and institute The left and right linear moving mechanism for driving the loading bracket side-to-side movement, the left and right are provided between the loading pedestal stated Direct mobile mechanism includes the left and right adjusting screw rod for moving left and right direction setting along the loading bracket, and the left and right is adjusted Section screw rod is screwed on the loading bracket, and one end of the left and right adjusting screw rod is coaxially fixed with left and right adjusting knob, The outside of the outer housing is arranged in the left and right adjusting knob.
4. a kind of fluorescent biochip detector as claimed in claim 3, it is characterised in that the front end of the loading bracket What face penetrated through before and after being provided with one slides chamber, and the rear end face of the loading platform is provided with one and matches with the chamber that slides Sliding block, the sliding block, which can slide up and down to be arranged in, described slides intracavitary, described sliding block and the loading bracket Between be provided with the linear moving mechanism up and down for driving the loading platform to move up and down, the linear moving mechanism up and down Including with the left and right adjusting screw rod adjusting shaft disposed in parallel, the adjusting shaft is located at the left and right adjusting spiral shell It on bar and is erected on the loading bracket, an adjustment gear is coaxially fixedly installed in the adjusting shaft, it is described Sliding block rear end face on be provided with one and be meshed with the adjustment gear rack gear, one end of the adjusting shaft is coaxially solid Surely it is provided with a up and down adjustment knob, the outside of the outer housing is arranged in the up and down adjustment knob.
5. a kind of fluorescent biochip detector as described in claim 1, it is characterised in that be provided in the outer housing One mounting platform, the mounting platform is located on the fluorescent biochip loader mechanism, on the mounting platform It is provided with hollow installation room, the LED light source mechanism includes LED light source, and the installation is arranged in the LED light source The rear end of room and the LED light source are camera to front irradiation, the image capturing mechanism, and the camera is vertically set The installation room is set on the installation room and protruded into, is provided on the installation room for the dress will to be seated in The image enlarger of biological specimen information amplification on the fluorescent biochip on carrying platform, the image amplification Mechanism is arranged between the camera and the loading platform, and the installation is indoor to be provided with for will be described The light that LED light source emits is projected to the light projection system of the image enlarger and the camera.
6. a kind of fluorescent biochip detector as claimed in claim 5, it is characterised in that the image enlarger is One eyepiece, the eyepiece is mounted vertically on the installation room and the eyepiece is co-axially located at the camera Lower section, the lower end of the eyepiece are stretched out the installation room and are located on the loading platform.
7. a kind of fluorescent biochip detector as claimed in claim 5, it is characterised in that the light projection system packet Convex lens, light transmitting sheet, refracting plates, the first optical filter and the second optical filter are included, the LED light source is arranged in the convex lens Front, the front of the convex lens is arranged in the light transmitting sheet, and the light transmitting sheet is arranged in the refracting plates Front, the refracting plates are obliquely installed upwards from front to back, the central light beam of the LED light source, the convex lens Center, the center of the light transmitting sheet, the refracting plates center be on same level optical axis, first optical filter And second optical filter every setting and is between the upper and lower the refraction between the camera and the eyepiece Piece is between first optical filter and second optical filter, the center of the refracting plates, first filter The center of mating plate and the center of second optical filter are on same vertical optical axis, the vertical optical axis and the mesh Mirror is coaxial.
8. a kind of fluorescent biochip detector as claimed in claim 5, it is characterised in that be provided in the outer housing Control circuit, the camera, the LED light source are electrically connected with the control circuit, are arranged on the outer housing There are a Datalink Interface and electric power connection interface, the Datalink Interface and the electric power connection interface and the control Circuit electrical connection processed.
9. a kind of fluorescent biochip detector as claimed in claim 8, it is characterised in that on the Datalink Interface It is connected with the display shown for image.
10. a kind of fluorescent biochip detector as described in claim 1, it is characterised in that be arranged on the loading platform There is placing platform, is provided in the placing platform and is plugged into the storing for importing the fluorescent biochip Importing slot on platform.
CN201910102573.8A 2019-02-01 Fluorescent biochip detector Active CN109781689B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910102573.8A CN109781689B (en) 2019-02-01 Fluorescent biochip detector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910102573.8A CN109781689B (en) 2019-02-01 Fluorescent biochip detector

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Publication Number Publication Date
CN109781689A true CN109781689A (en) 2019-05-21
CN109781689B CN109781689B (en) 2024-07-02

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111272724A (en) * 2020-04-02 2020-06-12 深圳华因康基因科技有限公司 Fluorescence detection optical system

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CN105181700A (en) * 2015-09-29 2015-12-23 宁波大学 Biochip detector
CN205607861U (en) * 2016-04-29 2016-09-28 华东医药(杭州)基因科技有限公司 Biochip image device
CN210090306U (en) * 2019-02-01 2020-02-18 宁波大学 Fluorescent biochip detector

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200263137Y1 (en) * 2001-11-08 2002-02-02 세호로보트산업 주식회사 Automatic biochip analysis machine
CN2739621Y (en) * 2004-05-18 2005-11-09 南京大渊生物技术工程有限责任公司 Biochip detecting and identifying instrument
CN204422420U (en) * 2015-02-15 2015-06-24 南京江南永新光学有限公司 A kind of fluorescence biosensor chip pick-up unit
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Publication number Priority date Publication date Assignee Title
CN111272724A (en) * 2020-04-02 2020-06-12 深圳华因康基因科技有限公司 Fluorescence detection optical system
CN111272724B (en) * 2020-04-02 2023-09-05 深圳华因康基因科技有限公司 Fluorescence detection optical system

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