CN109776550A - A kind of new bio alkali cpd in marine fungi source and its preparing the application in food preservative - Google Patents
A kind of new bio alkali cpd in marine fungi source and its preparing the application in food preservative Download PDFInfo
- Publication number
- CN109776550A CN109776550A CN201910045696.2A CN201910045696A CN109776550A CN 109776550 A CN109776550 A CN 109776550A CN 201910045696 A CN201910045696 A CN 201910045696A CN 109776550 A CN109776550 A CN 109776550A
- Authority
- CN
- China
- Prior art keywords
- new bio
- alkali cpd
- bio alkali
- marine fungi
- food
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to food antiseptics and antibacterial field, disclose the new bio alkali cpd in a kind of marine fungi source and preparation method thereof and are preparing the application in food preservative.The structural formula of new bio alkali cpd such as formula (I) or (II) are shown.Such compound has significant antibacterial activity, can be used for preparing food preservative.Therefore, new bio alkali cpd provided by the invention has corrosion-resistant food applications prospect.
Description
Technical field
The invention belongs to food antiseptic and antibacterial field, in particular to the new bio in a kind of marine fungi source, which alkalizes, to be closed
Object and its preparing the application in food preservative.
Background technique
Food apoilage, which is primarily referred to as the effect based on microorganism, causes food quality to decline or lose edible value
All variation.The nutritional ingredient abundant that food itself contains most easily makes microbial growth and mass propagation, and eventually leads to
Food it is putrid and deteriorated.Therefore people attempt with various methods go prevent food spoilage, such as cryo-conservation, isolation air, drying,
Using preservative etc., wherein the most commonly and effectively method is exactly to add preservative to inhibit or kill microorganism, to reach
To corrosion-resistant purpose.Common anti-corrosion measure is addition food preservative, and food preservative is divided into chemical preservative, natural anticorrosion
Agent and compound preservative, wherein the good antimicrobial effect, performance of chemical preservative be stable, the reasons such as cheap, using the most
Extensively.
Novel Food Chemistry preservative is developed in the world, and the effect disquisition of preservative focuses mostly in its bacteriostasis.
With the fast development of national economy and the continuous improvement of living standards of the people, food additives tend to develop novel resist
The preservative that bacterium property is strong, security performance is high, cheap is just at inevitable trend.The present invention is generated by microbe fermentation method
Preservative raw material, new Type of Preservatives is further obtained in manually modified mode, by bacteriostatic experiment the results show that with
The related a variety of Grain-positives of food and negative bacteria have stronger killing effect, inhibit the growth of microorganism very fast,
It has no toxic side effect after people, animal food, and activity is very strong in acid condition, is suitable for most of acid foods, especially beverage
Anti-corrosion has good dissolubility and stability.And new food preservative is greatly mostly from natural products or preservative guide's chemical combination
The important sources of object.The natural products in marine fungi metabolite source has sustainability, environmentally friendly, metabolite
The features such as rich and varied is the novel source of novel organic molecule or anti-corrosion small molecule.The metabolite of fungi has extensive
The various actives such as physiological activity, such as antibacterial, antitumor, immunological regulation is anti-inflammatory, and enzyme inhibits.What is therefrom found is novel with antibacterial
Active metabolite is that the food preservative for developing marine source and application have important practical significance.
Summary of the invention
In order to overcome shortcoming and deficiency existing in the prior art, the primary purpose of the present invention is that it is true to provide a kind of ocean
The new bio alkali cpd in bacterium source.
Another object of the present invention is to provide a kind of applications of the new bio alkali cpd in above-mentioned marine fungi source.
The object of the invention is achieved through the following technical solutions:
A kind of new bio alkali cpd in marine fungi source, the structural formula such as formula of the new bio alkali cpd
(I) or shown in (II):
The new bio alkali cpd is to separate to obtain from the fermentation liquid of marine fungi Penicillium sp.JX-2-1
?；Marine fungi Penicillium sp.JX-2-1 was deposited in Guangdong Province's Microbiological Culture Collection on January 4th, 2019
The heart (GDMCC), deposit number are GDMCC No:60525.
A kind of preparation method of the new bio alkali cpd in above-mentioned marine fungi source, including following operating procedure:
S1. marine fungi Penicillium sp.JX-2-1 is accessed into seed culture medium, shaking table culture obtains seed training
Nutrient solution；
S2. seed culture fluid is accessed in fermentation medium, stationary culture, obtains tunning；
S3. thallus is obtained by filtration in tunning, thallus is extracted three times with methanol, and concentrated extracting solution obtains medicinal extract, medicinal extract
Crude extract is extracted with ethyl acetate to obtain, ethyl acetate extract is chromatographed with silica gel normal-phase chromatography and is separated；With petroleum ether/second
Acetoacetic ester is eluted, and is collected 10%~30% ethyl acetate/petroleum ether fraction, then use column chromatography for separation technology, is tied
Structure formula new bio alkali cpd as shown in formula (I) or (II).
Preferably, average every liter of seed culture fluid described in S1 includes following components: 30 grams of glucose, 1 gram of yeast extract,
2-5 grams of peptone, 1-3 grams of agar, 1-5 grams of sodium chloride, water 1L.
Preferably, the condition of shaking table culture described in S1 is 80~150rpm of shaking speed, and 25~30 DEG C are cultivated 5~15 days.
Preferably, fermentation medium described in S2 is solid rice fermentation culture medium, consisting of rice and seawater are according to 1:
1.2 volume ratio mixing.
Preferably, the condition of stationary culture described in S2 is 25~30 DEG C of standings 25~50 days.
Preferably, column chromatography for separation technology silica gel column chromatography, gel filtration chromatography, C-18 reversed phase column chromatography described in S3.
A kind of new bio alkali cpd in above-mentioned marine fungi source is preparing the application in food preservative.
Compared with prior art, the present invention has the following advantages and beneficial effects:
The present invention provides the alkaloid compound in a kind of marine fungi source, such compound, which has, significantly inhibits antibacterial work
Property, there is significant suppression to staphylococcus aureus, bacillus subtilis, Candida albicans, Escherichia coli or salmonella etc. respectively
System activity can be used for preparing preservative for food antiseptic.Therefore, alkaloid compound provided by the invention has food antiseptic
Application potential.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.
Unless stated otherwise, the present invention uses reagent, method and apparatus for the art conventional reagent, method and are set
It is standby.
Unless stated otherwise, agents useful for same and material of the present invention are commercially available.
Embodiment 1: the separation and identification of fungi Penicillium sp.JX-2-1
1, material: the endogenetic fungus sample of the mangrove autumn eggplant leaf acquired from Guangzhou Nansha District seaside park.
2, by the culture of bacterial strain, separation, identification, the endogenetic fungal bacterial strain of pure mangrove autumn eggplant leaf is obtained, by mirror
It is set to mould fungi Penicillium sp.JX-2-1.Mould fungi Penicillium sp.JX-2-1 was on January 4th, 2019
It is deposited in Guangdong Province's Culture Collection (GDMCC), deposit number is GDMCC No:60525, and classification naming is
Penicilliumsp..The address of depositary institution is the compound the 59th of Xianlie Middle Road, Guangzhou City 100 5 building, building.
Embodiment 2: the preparation of new bio alkali cpd
1, separation prepares new bio alkali cpd from mould fungi Penicillium sp.JX-2-1 metabolite,
Steps are as follows:
(1) seed culture of mould fungi Penicillium sp.JX-2-1:
Culture medium composition includes following components by average every liter: 30 grams of glucose, 1 gram of yeast extract, and peptone 2-5
Gram, 1-3 grams of agar, 1-5 grams of sodium chloride, water 1L；
Culture medium is made test tube slant, and picking bacterial strain accesses inclined-plane, 30 DEG C shaking table culture 3 days, obtain seed culture fluid.
(2) fermented and cultured of mould fungi Penicillium sp.JX-2-1:
It is 1:1.2 mixing that solid rice fermentation culture medium prescription, which is rice and seawater according to volume ratio,；
Bacterial strain in seed liquor is transferred in solid rice fermentation culture medium, controls 25~30 DEG C of temperature always, culture
Time 25~50 days.
(3) above-mentioned hypha,hyphae methanol of having cultivated is extracted three times, concentrated extracting solution utilizes the concentrated extract of acquisition
Column chromatography chromatogram separation；10~30% ethyl acetate/petroleum ether eluents are collected, then with columns layers such as silica gel, gel, C-18 reverse phases
Analysis isolation technics is separated, and new bio alkali cpd is obtained.
2, the structured testing parsing of new bio alkali cpd
Structured testing parsing is carried out to new bio alkali cpd obtained above, obtains following tests data:
New bio alkali cpd: C18H14N2O5；HRESIMS:338.0907 [M+H]+(calculated value 338.0903)；
m.p.174–175℃；One-dimensional nuclear magnetic resonance data:1HNMR (400MHz, CDCl3) and carbon spectrum13CNMR(100MHz,CDCl3) such as
The following table 1.
By identification, which is new bio alkali cpd, and structural formula is as follows:
1 Novel antibacterial active alkaloid nuclear magnetic data table of table
Embodiment 3: the bacteriostatic experiment of new bio alkali cpd
1, material:
Strain: Escherichia coli type strain (ATCC29522), staphylococcus aureus type strain (ATCC29213), withered grass bud
The false silk beads coccus type strain (ATCC10231) of spore bacillus, salmonella, white；
Positive control: Ciprofloxacin (bacterial controls object) and Fluconazole (fungi reference material)；
Solvent prepare: dimethyl sulfoxide (dimethyl smLfoxide, DMSO) be solvent (Sigma, St.Lo uis,
Mo.)。
2, experimental method --- measurement minimum inhibitory concentration (MIC)
(1) preparation of bacterial suspension:
Staphylococcus aureus, Escherichia coli, bacillus subtilis, salmonella are inoculated in meat soup and LB culture medium respectively
In, 37 DEG C are shaken bacterium for 24 hours；
One bacterium colony of Candida albicans picking, be added 1mL sand Borrow's culture medium, after mixing 35 DEG C shake bacterium for 24 hours~48h；Leaching
Picking several bacterium colonies MH broth dilution, Maxwell adjust than turbid instrument (DENSIMAT, Bio-Merieux company) and try coccus before use
Bacterial concentration is 0.5 maxwell reduced turbidity in managing, and is equivalent to 5 × 107,8Colony forming number (CFU)/mL bacteria containing amount, 1:10 dilution
It is spare, inoculation in 10min.
(2) it uses doubling dilution to carry out vitro Drug bacteriostatic activity in 96 orifice plates to determine with sterile broth dilution sample and sun
Property control drug to 4 times of final detectable concentrations of greatest hope, be added in detection hole, then carrying out 2 times to drug is serially diluted, and
Equipped with no drug control well, the bacterium solution diluted is added in all detection holes and in no drug control well, every hole institute bacteria containing amount is about
It is 5 × 105The hole cfu/.
3, experimental result
As a result the antibacterial MIC of new bio alkali cpd is measured50Value (g/mL) is shown in Table 2.
Bacteriostatic test result (the MIC of 2 new bio alkali cpd (I) of table50Value g/mL)
Bacteriostatic test result (the MIC of 3 new bio alkali cpd (II) of table50Value g/mL)
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (3)
1. a kind of new bio alkali cpd in marine fungi source, it is characterised in that: the knot of the new bio alkali cpd
Structure formula such as formula (I) or (II) are shown:
2. a kind of new bio alkali cpd in marine fungi source according to claim 1, it is characterised in that: described new
Type alkaloid compound is to separate to obtain from the fermentation liquid of marine fungi Penicillium sp.JX-2-1；Marine fungi
Penicillium sp.JX-2-1 is deposited in Guangdong Province's Culture Collection on January 4th, 2019, and deposit number is
GDMCC No:60525.
3. a kind of new bio alkali cpd in marine fungi source according to claim 1 or 2 is preparing food antiseptic
Application in agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910045696.2A CN109776550B (en) | 2019-01-17 | 2019-01-17 | Alkaloid compound derived from marine fungi and application thereof in preparation of food preservative |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910045696.2A CN109776550B (en) | 2019-01-17 | 2019-01-17 | Alkaloid compound derived from marine fungi and application thereof in preparation of food preservative |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109776550A true CN109776550A (en) | 2019-05-21 |
| CN109776550B CN109776550B (en) | 2021-08-10 |
Family
ID=66501633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910045696.2A Active CN109776550B (en) | 2019-01-17 | 2019-01-17 | Alkaloid compound derived from marine fungi and application thereof in preparation of food preservative |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109776550B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114957401A (en) * | 2021-01-28 | 2022-08-30 | 广东轻工职业技术学院 | Peptide compound derived from marine fungi as well as preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1088209A (en) * | 1992-07-20 | 1994-06-22 | 惠乐康基金会集团公司 | Heterogeneous ring compound |
| WO2006059400A1 (en) * | 2004-12-03 | 2006-06-08 | The Kitasato Institute | Antibiotics fki-2140, method of producing the same and strain producing the same |
| WO2008127663A1 (en) * | 2007-04-11 | 2008-10-23 | Teva Gyógyszergyár Zártköruen Muködo Részvénytársaság | Method for reducing impurity level in mycophenolic acid fermentation |
| CN107721990A (en) * | 2017-08-25 | 2018-02-23 | 中山大学 | The iso-indole ketone compounds in a kind of marine fungi source and preparation method thereof and the application in anti-inflammatory drug is prepared |
-
2019
- 2019-01-17 CN CN201910045696.2A patent/CN109776550B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1088209A (en) * | 1992-07-20 | 1994-06-22 | 惠乐康基金会集团公司 | Heterogeneous ring compound |
| WO2006059400A1 (en) * | 2004-12-03 | 2006-06-08 | The Kitasato Institute | Antibiotics fki-2140, method of producing the same and strain producing the same |
| WO2008127663A1 (en) * | 2007-04-11 | 2008-10-23 | Teva Gyógyszergyár Zártköruen Muködo Részvénytársaság | Method for reducing impurity level in mycophenolic acid fermentation |
| CN107721990A (en) * | 2017-08-25 | 2018-02-23 | 中山大学 | The iso-indole ketone compounds in a kind of marine fungi source and preparation method thereof and the application in anti-inflammatory drug is prepared |
Non-Patent Citations (2)
| Title |
|---|
| HONGJU LIU ET AL.: ""Polyketides with Immunosuppressive Activities from Mangrove Endophytic Fungus Penicillium sp. ZJ-SY2"", 《MARINE DRUGS》 * |
| T. O. DZHASHI ET AL.: ""A NEW ROUTE FOR THE SYNTHESIS OF ISOMERIC BENZO[b]FUROINDOLES"", 《CHEMISTRY QF HETEROCYCLIC COMPOUNDS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114957401A (en) * | 2021-01-28 | 2022-08-30 | 广东轻工职业技术学院 | Peptide compound derived from marine fungi as well as preparation method and application thereof |
| CN114957401B (en) * | 2021-01-28 | 2023-04-28 | 广东轻工职业技术学院 | Peptide compound from marine fungi and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109776550B (en) | 2021-08-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Corrado et al. | Antimicrobial evaluation of fungal extracts produced by endophytic strains of Phomopsis sp. | |
| CN106434783B (en) | A kind of chromone compound and preparation method thereof and the application in preparation antibacterials | |
| Arunachalam et al. | Studies on bioprospecting of endophytic bacteria from the medicinal plant of Andrographis paniculata for their antimicrobial activity and antibiotic susceptibility pattern | |
| CN101153287B (en) | Streptomycete antimicrobial production and method of preparing the same | |
| Wu et al. | Two new alkylated furan derivatives with antifungal and antibacterial activities from the plant endophytic fungus Emericella sp. XL029 | |
| CN110684064B (en) | Compound with antibacterial activity and preparation method and application thereof | |
| CN105130963A (en) | Separation and extraction and structure identification of novel quorum sensing inhibitor and application thereof | |
| CN103255061A (en) | Penicillium griseofulvum, antibacterial active compound generated thereby and application | |
| CN108315264A (en) | A kind of polyketide in sea paint endogenetic fungus source and its application in preparing anti-inflammatory drug | |
| CN103834585B (en) | The rhizosphere pseudomonas of high yield phenazine-1-carboxylic acid and azophenlyene-1-acid amides | |
| US20230175033A1 (en) | Production of jasmonates in filamentous fungi | |
| CN109776550A (en) | A kind of new bio alkali cpd in marine fungi source and its preparing the application in food preservative | |
| US20230313247A1 (en) | Kind of antimycin compound and methods of making and using it | |
| CN109400444B (en) | Sesquiterpenoids for inhibiting plant pathogenic fungi and preparation method thereof | |
| CN104059044B (en) | A kind of Xanthone derivative and preparation method thereof and application | |
| CN103408550A (en) | 2,5-diketopiperazine dipeptide derived from lysobacter enzymogenes as well as preparation method and application thereof | |
| CN102659547B (en) | Ophiobolin sesterterpene compound and preparation and application thereof | |
| CN103232940B (en) | Bionectria ochroleuca Bo-1 strain, cultures thereof, and applications of the strain and the cultures thereof in resisting pathogenic bacteria | |
| CN102659734B (en) | Triene antibiotic, preparation method thereof and application thereof | |
| CN108794502B (en) | Trichothecene compound and preparation method and application thereof | |
| Srivastava et al. | Enhanced production of phytotoxic polyketides isolated from Curvularia lunata by applying chemical stresses | |
| CN106472572A (en) | A kind of microbial bactericide and preparation method and application | |
| CN107739740A (en) | A kind of preparation method and application of the Lasiodiplodins compounds in marine fungi source | |
| CN113773966B (en) | Alternaria tenuissima and application thereof | |
| CN102604843A (en) | Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |