CN102604843A - Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases - Google Patents
Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases Download PDFInfo
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- CN102604843A CN102604843A CN2012100953198A CN201210095319A CN102604843A CN 102604843 A CN102604843 A CN 102604843A CN 2012100953198 A CN2012100953198 A CN 2012100953198A CN 201210095319 A CN201210095319 A CN 201210095319A CN 102604843 A CN102604843 A CN 102604843A
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Abstract
The invention relates to a preparation method of a fungus fermentation product and application of the fungus fermentation product in prevention and treatment of rice diseases, which belong to the field of biological prevention and treatment. The preparation method of the fungus fermentation product comprises liquid culture of fungus of Agrocybe sp. YB2005, treatment of the fermentation product, and separation of the fermentation product. The fungus fermentation product prepared by the method disclosed by the invention has better effects of prevention and treatment of rice bacterial leaf streak, rice sheath blight, and rice blast, and can be prepared by fermentation of a strain of the Agrocybe sp. YB2005, wherein culture medium raw materials have extensive sources. The preparation method of the fungus fermentation product is simple and easy for realization of industrial production.
Description
Technical field
The present invention relates to a kind of preparation method of fungus fermentation products, can be used for the control of rice disease, belong to the biological control field.
Background technology
Macro fungi is not only of a great variety, and is " creation coefficient " very high biological group.The natural product structure of finding in the macro fungi is various, and have antibacterium, promote that NGFF is synthetic, enzyme inhibitors, antimycotic, cell toxicant, receptor antagonist and multiple biological activity such as anti-oxidant.
Basidiomycetes is very close with human relation.Found some plant to antitumor with prevent effect is arranged aspect the coronary heart disease after, received the attention of the world of medicine and caused great interest.The physiologically active substance that basidiomycetes produces has polyose, polypeptide and protein, antibiotics, vitamins etc.; Wherein antibiotics comprises the polyacetylene compounds; Like agrocybin (Agrocybin); HOCH2 (C ≡ C) 3CONH2 derives from Agrocybe cylindracea, and gram-positive microorganism, negative bacterium and fungi are had antagonistic action.
Microbial pesticide comprises agricultural antibiotic and living microorganism two big classes.Wherein agricultural antibiotic is the secondary metabolites with agricultural chemicals function that is produced by the antibiotic bacteria fermentation, and it is the chemical substance that clear and definite molecular structure is arranged.Agricultural antibiotic is undoubtedly an important composition in the agricultural chemicals.Because it comes from biology, so more and more receive people's concern.At present, aspect the biological control of insect, fungi is research, produces and use before maximum biological groups.The Mycophyta biological pesticide mainly is living fungi, and that puts down in writing in the world at present has 100 to belong to approximately, and kind more than 800, major part are facultative or obligate pathogen.The research of fungus insecticide has long historical in China, the researchs such as security, control pine moth and Pyrausta nubilalis (Hubern). of preparation are all done a lot of work, but the suitability for industrialized production technology is unresolved so far, thereby does not have formal commodity to come out all the time.
Exploitation has the environment friendly agricultural of great diseases such as control rice blast, hypochnus and bacterial stripe; To protecting rice field wetland function and reducing the rice field, also can promote the development of the ecological agriculture and green agriculture because chemical pesticide uses the pollution of area source that causes significant.Based on above background, carry out research of the present invention.
Summary of the invention
The object of the present invention is to provide a kind of preparation method and the application in the rice disease control of fungus fermentation products.
The present invention at first provides a kind of preparation method of fungus fermentation products, and said preparation method comprises:
1) liquid culture and tunning are handled: with fungi
Agrocybe sp.Carry out liquid submerged fermentation after the YB2005 activation, the culture medium prescription of liquid submerged fermentation is by weight being: yam 16-25%, glucose 1.5-2.5%; Peptone 0.3-0.7%; Surplus is a water, pH nature, 0.1Mpa, 121 ℃ of sterilization 30min; Place 25-28 ℃, cultivated 7-12 days in the 180-230r/min constant temperature shaking table; After the fermentation ends, with centrifuging mycelium is separated with fermented liquid, fermented liquid is used ethyl acetate extraction, and extraction liquid obtains organic crude extract medicinal extract through dehydration, concentrated;
2) product separation: with 1) described organic crude extract medicinal extract is used dissolve with methanol, and through gel filtration chromatography, methyl alcohol is eluent; Elutriant is measured antibacterial substance with the TLC-bioautography, merges to have active component, and active ingredient is used dissolve with methanol; Carry out reversed-phase silica gel column chromatography, with different gradient methanol-water wash-outs, elutriant is followed the trail of with the TLC-bioautography is active; Collect active ingredient; Then go up silicagel column, with sherwood oil-acetone gradient elution, the product that TLC-bioautography collection active ingredient obtains having anti-microbial activity is fungus fermentation products;
The fungi that is set forth in
Agrocybe sp.YB2005 has been preserved in Chinese typical culture collection center on March 12nd, 2012
, deposit number CCTCC NO:2012076.
The culture medium prescription of said liquid submerged fermentation is by weight being: yam 20%, and glucose 2%, peptone 0.5%, surplus is a water.
In the said TLC-bioautography, TLC adopts chloroform: the methyl alcohol volume ratio is for being the developping agent of 10:1; Concrete operations are: in developping agent, launch behind the point sample, cover on the culture plate that is mixed with paddy rice slice disease pathogen bacterium thin layer plate is counter then, after 20 minutes, remove thin layer plate, culture plate is placed 28 ℃ of cultivations, observe antibacterial situation after 3 days.
Step 2) with different gradient methanol-water wash-outs, wherein the shared volume ratio of methyl alcohol is respectively described in: 10%, 20%, 30%, 40%, 50%.
Step 2) silicagel column described in adopt sherwood oil-acetone volume score Wei 7:1, the gradient elution of 6:1,5:1.
The present invention also provides the application of described fungus fermentation products in the rice disease control.
Wherein said rice disease is that rice blast, rice sheath blight disease or paddy rice slice are sick.
The fungus fermentation products of the present invention's preparation has the better prevention effect to paddy rice slice disease, rice sheath blight disease and rice blast, can utilize bacterial strain of the present invention
Agrocybe sp.The YB2005 fermentation makes, the culture medium raw material wide material sources, and the preparation method is simple, easy realization of industrial production.
Description of drawings
Fig. 1 compound 8-hydroxy-2,4, the monocrystalline X-Ray diffractogram of 6-octatriynamide.
Fig. 2 compound 8-hydroxy-2,4,6-octatriynamide's
1H-NMR figure.
Fig. 3 compound 8-hydroxy-2,4,6-octatriynamide's
13C-NMR figure.
Fig. 4 compound 8-hydroxy-2,4, the anti-rice blast effect of 6-octatriynamide; Wherein A is that the stripped nutritive medium of blade adds 50ug/mL 8-hydroxy-2,4,6-octatriynamide; B is that blade exsomatizes nutritive medium interpolation 50ug/mL DMSO as solvent control, and C is that the stripped nutritive medium interpolation of blade pure water is contrast, and arrow is represented the rice blast scab.
Fig. 5 is compound 8-hydroxy-2,4, and 6-octatriynamide is to the restraining effect of paddy rice slice disease pathogen bacterium; Annotate: 1-9 representes that respectively compound concentration is respectively 250 μ g/mL, 125 μ g/mL, 62.5 μ g/mL, 31.3 μ g/mL, 15.6 μ g/mL, 7.81 μ g/mL, 3.91 μ g/mL, 1.95 μ g/ mL and 0.98 μ g/mL; 10 expressions supply examination bacterium+substratum to be contrast, and 11 expression equivalent methyl alcohol+confession examination bacterium+substratum is contrast.
Fig. 6 compound 8-hydroxy-2,4,6-octatriynamide is to the restraining effect of rice sheath blight disease pathogenic bacteria; Annotate: A is a control group: the rice sheath blight disease mycelia is not adding said compound 8-hydroxy-2, and 4, the PDA solid culture basal growth of 6-octatriynamide; B is an experimental group: the rice sheath blight disease mycelia is adding said compound 8-hydroxy-2, and 4, the growth of the PDA solid medium of 6-octatriynamide is suppressed.
Embodiment
Fungus fermentation products provided by the invention the preparation method, the steps include:
1) liquid culture and tunning are handled:With fungi
Agrocybe sp.YB2005 (be preserved on March 12nd, 2012 Chinese typical culture collection center (CCTCC), address: Wuhan Wuhan University, deposit number CCTCC NO:2012076) slant strains is inoculated in activation in the triangular flask that 150mL yam liquid nutrient medium is housed earlier; Activation condition is rotating speed 230 r/min, 28 ℃ of culture temperature, incubation time 7 days; Carry out large batch of liquid submerged fermentation again, adopt culture medium prescription to be: to contain yam 200g in every premium on currency, glucose 20g; Peptone 5 g, pH nature, 0.1Mpa; 121 ℃ of sterilization 30min place 25-28 ℃, cultivate in the 180-230 r/min constant temperature shaking table.Ferment after 7-12 days, mycelium is separated with fermented liquid with centrifuging.Fermented liquid is used equal volume of ethyl acetate, and the gained extract is used anhydrous sodium sulfate dehydration, uses Rotary Evaporators vacuum concentration under 40 ℃ of conditions again, obtains organic crude extract medicinal extract.
2) compound separation:With 1) described organic crude extract medicinal extract uses dissolve with methanol, and through gel filtration chromatography, gel adopts Sephadex LH-20; Consumption is applied sample amount 70-700 times, and methyl alcohol is eluent, and flow velocity is approximately 10-15 s/drop; Every test tube is collected 4-8 mL, measures antibacterial substance (Zhao Jianglin etc., anti-microbial activity composition in TLC-bioautography-mtt assay detection Rhizoma Paridis endogenetic fungus with the TLC-bioautography; Research and development of natural products, 2008 01 phases) (concrete operations are: with each test tube sample point sample on the GF254 silica gel thin-layer plate, at chloroform: methyl alcohol is to launch in the developping agent of 10:1; After drying up solvent, the anti-lid of this thin layer plate is being mixed with on the paddy rice slice pathogenetic bacteria culture plate, after 20 minutes; Remove thin layer plate, culture plate is put 28 ℃ of cultivations, observe antibacterial situation after 3 days; Merge active substance according to antibacterial situation) each collection test tube is suppressed paddy rice slice disease pathogen bacterium activity experiment, merging has active component.Active ingredient is used an amount of dissolve with methanol, carry out reversed-phase silica gel column chromatography (reverse phase silica gel is used RP18, and consumption is applied sample amount 40-800 times); With different gradient methanol-water eluent wash-outs, said methanol-water gradient is: 10%, 20%, 30%, 40%, 50%, follow the trail of with the TLC-bioautography is active; Collect active ingredient, then use silica gel column chromatography, with sherwood oil-acetone (7:1; 6:1; 5:1) gradient elution, the TLC-bioautography is collected active ingredient and is obtained compound, is further purified the pure article that obtain.
Pure article are carried out monocrystalline X-Ray (monocrystalline X-ray diffraction) and NMR (nucleus magnetic resonance) analysis, and carry out biological activity test.
According to X-Ray diffraction data and NMR (nucleus magnetic resonance) data, compound is carried out structure identify, can confirm the structure of compound.
Compound of the present invention is three acetylene compound 8-hydroxy-2,4, and 6-octatriynamide, molecular formula is C
8H
5NO
2, structural formula is following:
According to compound 8-hydroxy-2; 4, the biological activity determination of 6-octatriynamide, finding all has control effect to rice blast, slice germ and hypochnus;, rice leaf adds compound 8-hydroxy-2 in exsomatizing nutritive medium; 4,6-octatriynamide can suppress the generation of rice blast during for 50ug/mL at compound concentration.On 96 porocyte culture plates, compound 8-hydroxy-2,4,6-octatriynamide is 7.81 ug/mL (embodiment 4) to the sick minimum inhibition concentration of paddy rice slice.When being inoculated in, the rice sheath blight disease pathogenic bacteria contains the said compound 8-hydroxy-2 of 0.2mg/mL, and 4, the PDA substratum of 6-octatriynamide (culture medium prescription: yam 200g; Glucose 20g, peptone 5 g, agar 10 g; Water 1000mL, pH nature) on the flat board, the rice sheath blight disease growth of pathogenic bacteria is suppressed fully; When control group hypochnus pathogenic bacteria hyphal diameter was 8.1cm, the hypochnus pathogenic bacteria diameter of experimental group was merely 0.8cm (embodiment 5).
With potato glucose substratum (containing yam 200g in every premium on currency, glucose 20g, peptone 5 g, pH nature, 0.1Mpa, 121 ℃ of sterilization 30min), bacterial strain YB2005 liquid fermentation and culture 10 L with after the activation cultivate 9 d in 28 ℃ of shaking tables.After thalline filtered, fermented liquid was concentrated into 0.5 L, and fermented liquid is used ethyl acetate extraction, and the ETHYLE ACETATE part is filtered with dissolve with methanol with concentrating behind the anhydrous sodium sulfate dehydration again, gets the solvable crude extract of methyl alcohol (1.2 g, brown medicinal extract).
To go up 1.2 g methanol crude extract in the step, fully dissolve with an amount of methyl alcohol, through gel (140 g) column chromatography, methanol-eluted fractions, flow velocity is approximately 10-15 s/drop, and every test tube is collected 5 mL, and similar component merging obtains component Fr.4 (111 mg) respectively.
Get 50 mg among the Fr.4 from a last step (111 mg) and use an amount of dissolve with methanol, through reverse phase silica gel (10 g) column chromatography, 10% methanol-eluted fractions TLC detects and obtains main ingredient 38 mg; Then through gel column (40 g) chromatography, methanol-eluted fractions gets main ingredient 37 mg, then through the purification on normal-phase silica gel column chromatography; Sherwood oil-acetone (7:1,6:1,5:1) wash-out; Obtain than pure component 33 mg, further get pure article 16 mg of said compound through reverse phase silica gel (10 g) column chromatography and gel (40 g) column chromatography.Part of compounds is carried out biological activity test.
The compound of a last step gained is got 5 mg with acetone water 4:1 dissolving, put in the sample bottle, be placed in 4 ℃ of refrigerators, obtain the crystal of said compound after 2 weeks.Get suitable crystal and carry out the X-Ray diffraction.
The measuring method of compound structure adopts the X-Ray diffraction analysis, can confirm the structure of compound.Its monocrystalline X-Ray diffractogram is seen accompanying drawing 1.
Similar with embodiment 1, its difference is that compound structure evaluation employing NMR (nucleus magnetic resonance) resolves, and according to compound N MR and MS data, can confirm compound structure.
From compound
138 carbon are arranged in its structure of C-NMR data presentation, infer that according to chemical displacement value it is 6 alkynes carbon, 1 even oxygen methylene radical and 1 acyl ammonia carbon (d 153.9s).With MeOD tritium for reagent
1H-NMR shows has 2 same carbonaceous that chemical displacement value is identical in its structure, be tritium with DMSO for reagent
1H-NMR also shows same carbonaceous that has 2 chemical displacement values identical in its structure, and ((s, 1H), (s is 1H) with d 5.63 (t, 6.1,1H)) for d 7.97 for d 8.42 to also have three reactive hydrogens.Long-range relevant according to H-C (8) with the HMBC of C (7), C (6), C (5) and C (4), confirmed the structure of compound, long-range relevant this structure of also supporting of the HMBC of three reactive hydrogens.
The quasi-molecular ion peak that ESI-MS records compound does
M/z148.3 [
M+ H]
+With
M/z170.1 [
M+ Na]
+In conjunction with the NMR data, confirm compound molecule formula C
8H
5NO
2
In sum,, confirm that compound is 8-hydroxy-2 in conjunction with the three-dimensional arrangement of monocrystalline X-Ray diffraction (Fig. 1) assay determination, 4,6-octatriynamide.Compound
1H-NMR with
13The C-NMR data are seen table 1 and Fig. 2-3.
Table 1. compound 8-hydroxy-2,4,6-octatriynamide NMR spectral data
Getting fresh rice leaf cultivates respectively at substrate nutritive medium (substrate nutrient solution composition: benzoglyoxaline 200mg; Pure water 1L) and to add in the substrate nutritive medium of 50ug/mL DMSO solvent be control group; Other gets fresh rice leaf and cultivates at interpolation 50ug/mL compound 8-hydroxy-2; 4, the substrate nutrient solution of 6-octatriynamide is an experimental group, and each treatment group is all inoculated the former bacterium of rice blast respectively.Through cultivating, the result shows that typical rice blast scab appears in (Fig. 4) control group, adds the compound group and has only nature withered and yellow, and no scab occurs, and shows disease resistance.Explanation adds compound 8-hydroxy-2 in rice leaf exsomatizes nutritive medium, and 4,6-octatriynamide can suppress the generation of rice blast when compound concentration is 50 ug/mL.
With described compound 8-hydroxy-2,4,6-octatriynamide is dissolved in the methyl alcohol earlier; Be added to beef extract-peptone liquid nutrient medium (prescription: Carnis Bovis seu Bubali cream 3 g again; Peptone 10 g, sodium-chlor 5 g, water 1000 mL; PH 7.0-7.2) in; Making compound concentration is 0.25 mg/mL, adopts 96 porocyte plates to carry out half coubling dilution and dilutes said compound, makes every hole concentration be respectively 250 μ g/mL, 125 μ g/mL, 62.5 μ g/mL, 31.3 μ g/mL, 15.6 μ g/mL, 7.81 μ g/mL, 3.91 μ g/mL, 1.95 μ g/ mL, 0.98 μ g/mL; Each concentration gradient is established three repetitions, and (bacterial concentration is 10 to sterilization back inoculation paddy rice slice disease pathogen bacterium 10 μ L
6Individual/mL), and establish equivalent methyl alcohol+confession examination bacterium+substratum and supply examination bacterium+substratum to be contrast.96 orifice plates are put 28 ℃ cultivate 36 h.1-6 nutrient solution clear wherein, asepsis growth; The 7-11 nutrient solution is muddy, and bacteria growing is arranged.What experiment showed said compound is 7.81 μ g/mL (Fig. 5) to the minimum inhibition concentration of paddy rice slice disease pathogen bacterium.
With said compound 8-hydroxy-2; 4,6-octatriynamide joins in the PDA solid medium, and making its concentration is 0.2 mg/mL; With the PDA solid medium that does not add described compound is contrast; All make culture plate after the sterilization, in the middle of dull and stereotyped, inoculate the rice sheath blight disease pathogenic bacteria, cultivated 5 days for 28 ℃.Experimental result shows that the rice sheath blight disease growth of pathogenic bacteria is suppressed fully, when control group hypochnus pathogenic bacteria hyphal diameter is 8.1cm, and the hypochnus pathogenic bacteria of experimental group do not grow (Fig. 6).
Claims (7)
1. the preparation method of a fungus fermentation products, it is characterized in that: said preparation method comprises:
1) liquid culture and tunning are handled: with fungi
Agrocybe sp.Carry out liquid submerged fermentation after the YB2005 activation, the culture medium prescription of liquid submerged fermentation is by weight being: yam 16-25%, glucose 1.5-2.5%; Peptone 0.3-0.7%; Surplus is a water, pH nature, 0.1Mpa, 121 ℃ of sterilization 30min; Place 25-28 ℃, cultivated 7-12 days in the 180-230r/min constant temperature shaking table; After the fermentation ends, with centrifuging mycelium is separated with fermented liquid, fermented liquid is used ethyl acetate extraction, and extraction liquid obtains organic crude extract medicinal extract through dehydration, concentrated;
2) product separation: with 1) described organic crude extract medicinal extract is used dissolve with methanol, and through gel filtration chromatography, methyl alcohol is eluent; Elutriant is measured antibacterial substance with the TLC-bioautography, merges to have active component, and active ingredient is used dissolve with methanol; Carry out reversed-phase silica gel column chromatography, with different gradient methanol-water wash-outs, elutriant is followed the trail of with the TLC-bioautography is active; Collect active ingredient; Then go up silicagel column, with sherwood oil-acetone gradient elution, the product that TLC-bioautography collection active ingredient obtains having anti-microbial activity is fungus fermentation products;
The fungi that is set forth in
Agrocybe sp.YB2005 has been preserved in Chinese typical culture collection center, deposit number CCTCC NO:2012076 on March 12nd, 2012.
2. the preparation method of fungus fermentation products according to claim 1, it is characterized in that: the culture medium prescription of said liquid submerged fermentation is by weight being: yam 20%, glucose 2%, peptone 0.5%, surplus is a water.
3. the preparation method of fungus fermentation products according to claim 1, it is characterized in that: in the said TLC-bioautography, TLC adopts chloroform: the methyl alcohol volume ratio is for being the developping agent of 10:1; Concrete operations are: in developping agent, launch behind the point sample, cover on the culture plate that is mixed with paddy rice slice disease pathogen bacterium thin layer plate is counter then, after 20 minutes, remove thin layer plate, culture plate is placed 28 ℃ of cultivations, observe antibacterial situation after 3 days.
4. the preparation method of fungus fermentation products according to claim 1 is characterized in that: step 2) described in different gradient methanol-water wash-outs, wherein the shared volume ratio of methyl alcohol is respectively: 10%, 20%, 30%, 40%, 50%.
5. the preparation method of fungus fermentation products according to claim 1 is characterized in that: step 2) described in silicagel column adopt sherwood oil-acetone volume score Wei 7:1, the gradient elution of 6:1,5:1.
6. like arbitrary the application of described fungus fermentation products in the rice disease control among the claim 1-5.
7. the application of fungus fermentation products according to claim 6 is characterized in that: said rice disease is that rice blast, rice sheath blight disease or paddy rice slice are sick.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102993030A (en) * | 2012-12-05 | 2013-03-27 | 福建师范大学 | Benzyne compound and preparation method thereof, as well as application in control of rice pathogens |
| CN115381928A (en) * | 2022-10-27 | 2022-11-25 | 山东中医药大学附属医院 | Application of fungus extract in preparation of medicine for treating ulcerative colitis |
-
2012
- 2012-03-31 CN CN2012100953198A patent/CN102604843A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 20081231 赵江林等 TLC-生物自显影-MTT法检测滇重楼内生真菌中抗菌活性成分 28-32,51 1－7 , 第1期 * |
| 赵江林等: "TLC-生物自显影-MTT法检测滇重楼内生真菌中抗菌活性成分", <天然产物研究与开发> * |
| 郑永标: "5种食药用大型真菌天然产物的研究", <中国博士学位论文全文数据库 农业科技辑> * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102993030A (en) * | 2012-12-05 | 2013-03-27 | 福建师范大学 | Benzyne compound and preparation method thereof, as well as application in control of rice pathogens |
| CN102993030B (en) * | 2012-12-05 | 2014-03-12 | 福建师范大学 | Benzyne compound and preparation method thereof, as well as application in control of rice pathogens |
| CN115381928A (en) * | 2022-10-27 | 2022-11-25 | 山东中医药大学附属医院 | Application of fungus extract in preparation of medicine for treating ulcerative colitis |
| CN115381928B (en) * | 2022-10-27 | 2022-12-27 | 山东中医药大学附属医院 | Application of fungus extract in preparation of medicine for treating ulcerative colitis |
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Application publication date: 20120725 |