CN109769860A - Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application - Google Patents
Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application Download PDFInfo
- Publication number
- CN109769860A CN109769860A CN201910186476.1A CN201910186476A CN109769860A CN 109769860 A CN109769860 A CN 109769860A CN 201910186476 A CN201910186476 A CN 201910186476A CN 109769860 A CN109769860 A CN 109769860A
- Authority
- CN
- China
- Prior art keywords
- ippmhbc
- metarhizium anisopliae
- locust
- composite bacteria
- bacteria agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to field of biotechnology, and in particular to a kind of Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application.Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC-009, deposit number are CGMCC No.16000, have good LD50 time and bombys batryticatus rate for Oedaleus asiaticus B, Dasyhippus barbipes.Green muscardine fungus IPPMHBC-009 and chlopyrifos are administered in combination the present invention, can significantly improve the quick-acting and killing rate of desinsection.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and
Using.
Background technique
Locust is the disastrous insect of Orthoptera locust section, and the plague of locusts frequently occurs in China, causes to China's agricultural huge
Injury.Currently, China control grassland grasshopper harm means mainly use chemical prevention method, have quickly, efficiently, make
With it is flexible the advantages that, still, as people increasingly pay attention to the sustainable development of environmental economy, biological locust elimination method is developed
Come and applies in real work.Biology goes out locust method, especially green muscardine fungus, muscardine etc., has certain specificity, to people
It raises harmless, while also having many advantages, such as that free from environmental pollution, noresidue, pest will not develop drug resistance.
Although disinsection fungal, which prevents and treats locust, has certain control efficiency, fungi is knocked down, killing off the insect pests acts on slowly,
It needs just be worked after a period of time, is suitble to general generation time control and application, and in locust outbreak year part, or part
When regional serious generation, disinsection fungal cannot quickly reduce insect density in the short time.Therefore, how to utilize disinsection fungal short
The quantity of control locust is agriculturally urgent problem to be solved in time.
Bacterial strain and agriculture chemical compounding are that raising locust control effect explores approach, but bacterial strain and pesticide at present
Compounding effect is unstable, and compounding is improper to fail to improve instead even reduction locust control effect.
Summary of the invention
In order to obtain effective bacterial strain and agriculture chemical compounding formula, the present invention provides a kind of Metarhizium anisopliae IPPMHBC-
009 composite bacteria agent and its application.
The object of the present invention is to provide the composite bacteria agents comprising Metarhizium anisopliae IPPMHBC-009 and chlopyrifos.
A further object of the present invention is to provide the applications of Metarhizium anisopliae IPPMHBC-009 composite bacteria agent.
A further object of the present invention is to provide use Metarhizium anisopliae IPPMHBC-009 composite bacteria agent to carry out anti-locust elimination
The method of worm.
Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC- of specific embodiment according to the present invention
The deposit number CGMCC No.16000 of 009 bacterial strain, the bacterial strain were stored in China General Microbiological bacterium on 08 02nd, 2018
Kind collection, collection address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
The Metarhizium anisopliae IPPMHBC-009 composite bacteria agent of specific embodiment according to the present invention, it includes chafers
Green muscardine fungus IPPMHBC-009 and chlopyrifos.
The Metarhizium anisopliae IPPMHBC-009 composite bacteria agent of specific embodiment according to the present invention, Metarhizium anisopliae
IPPMHBC-009 and the mass ratio of chlopyrifos are 1:1000~2000.
The Metarhizium anisopliae IPPMHBC-009 composite bacteria agent of specific embodiment according to the present invention, the concentration of chlopyrifos
For 0.004~0.1ppm, the mass concentration of green muscardine fungus IPPMHBC-009 is 0.0064%~0.16%.When with bacteriostat, poison with poison
Tick is diluted to normal concentration with acetone, and green muscardine fungus IPPMHBC-009 conidia powder and wheat bran are hybridly prepared into regulation according to mass ratio
Concentration.
Green muscardine fungus IPPMHBC-009 of the invention is 3.87d to the median lethal time of Dasyhippus barbipes, and bombys batryticatus rate is
91.67%, the median lethal time to Oedaleus asiaticus B is 3.55d, and bombys batryticatus rate is 93.33%, therefore, green muscardine fungus of the invention
IPPMHBC-009 is with good application prospect.
Metarhizium anisopliae IPPMHBC-009 and chlopyrifos combination can be proposed the quick-acting for killing locust by the present invention significantly
And killing rate, it is lethal slow not only to solve the problems, such as fungus insecticide, and largely reduce pollution, alleviates pest pair
Chemical pesticide develops drug resistance problem.
Detailed description of the invention
Fig. 1 shows 9 kinds of green muscardine fungus to the correction cumulative mortality curve of Dasyhippus barbipes 15d；
Fig. 2 shows 9 kinds of green muscardine fungus to the correction cumulative mortality curve of Oedaleus asiaticus B 15d；
Fig. 3 shows the cumulative mortality of Oedaleus asiaticus B after processing, F: only application chlopyrifos；B: only application green muscardine fungus
IPPMHBC-009；G: mixing chlopyrifos and green muscardine fungus IPPMHBC-009.
The deposit number CGMCC of Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC-009 bacterial strain
No.16000, the bacterial strain were stored in China General Microbiological Culture Collection Center on 08 02nd, 2018, collection address:
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
Specific embodiment
The source of test worm of the present invention:
1. Dasyhippus barbipes (Dasyhippus barbipes Fischer-Waldheim): 2~3 age nymphs of a locust, method of beating
It is collected in In Xilingol League In Inner Mongolia white clouds coulomb locust area.
2. Oedaleus asiaticus B (Oedaleus decorus asiaticus B.Bienko): 2~3 age nymphs of a locust, the method for beating are adopted
Combine in the In Xilingol League In Inner Mongolia west northern 7 kilometers of areas Chu Huang of black flag Bayan Gol.
The locust raising of acquisition feeds suitable fresh sheep's hay in the big frame in experiment station dedicated for supporting locust daily
Blade.For guarantee test effect, test the previous day Nature enemy.
Bioassay of the 1 Metarhizium anisopliae IPPMHBC-009 of embodiment to Dasyhippus barbipes nymph of a locust's pathogenicity
Metarhizium anisopliae IPPMHBC-009 is isolated from soil, and deposit number is CGMCC No.16000.
9 kinds of Metarhizium Strains are measured to the virulence effect of locust using stomach toxicity method.
0.2g muscardine spore powder is weighed first to be added in 2.0g wheat bran, adds 0.1ml edible oil, is sufficiently stirred
It is even.Then it weighs the green muscardine fungus wheat bran bait formulation that 0.2g is prepared to be put into culture dish, is put into togerther cover glass plate together with culture dish
Worm basket, number are supported, then is put into 20 3 age Dasyhippus barbipes nymphs of a locust of the same size in feeding worm basket.Blank control group wheat bran bait formulation
In muscardine spore powder is not added.The present invention selects 9 plants of muscardine spore powder and a control CK, every kind of conidia powder to be arranged 3 times
It repeats, i.e., totally 33 processing, in being raised in insectary.Bait formulation was taken out in second day, feeds suitable fresh sheep's hay daily later,
And the death condition of every basket of nymph of a locust is investigated, it makes a record respectively.
The dead nymph of a locust is sorted out and is respectively put into the culture dish for being lined with filter paper of reference numeral, certain humidity is kept, makes
Grow bombys batryticatus.Every kind of bacterium is investigated after the nymph of a locust to be tested is all dead treated the bombys batryticatus rate of the nymph of a locust, 15 days after combination processing
Interior daily cumulative mortality and median lethal time (LT50) pick out to the preferable strain of locust lethal effect.
9 plants of Metarhizium Strains are shown in figure l, table 1 to the bioassay results of 3 age Dasyhippus barbipes nymph of a locust pathogenicities.
Comparison of the 19 plants of green muscardine fungus of table to Dasyhippus barbipes pathogenicity
Analyzed by table 1 it is found that above-mentioned 9 kinds of strains testeds have to the nymph of a locust it is pathogenic, median lethal time 3.87d~
Between 8.83d, bombys batryticatus rate is between 43.33%~91.67%, the wherein median lethal time of Metarhizium Strains IPPMHBC-009
For 3.87d, bombys batryticatus rate is 91.67%, and 803,200614, the bacterial strains median lethal time such as 2010-10 is slightly long, bombys batryticatus rate is lower.
Therefore, compared with the bacterial strains such as 803,200614, there are significant differences by Metarhizium Strains IPPMHBC-009, to Dasyhippus barbipes
There is higher pathogenicity.
Bioassay of the 2. Metarhizium anisopliae IPPMHBC-009 of embodiment to Oedaleus asiaticus B pathogenicity
9 plants of bacterial strains are measured to the power of curing the disease of Oedaleus asiaticus B, method of the measuring method with embodiment 1.
9 plants of Metarhizium Strains are shown in Fig. 2, table 2 to the bioassay results of Oedaleus asiaticus B pathogenicity.
Comparison of the 29 plants of green muscardine fungus of table to Oedaleus asiaticus B pathogenicity
Analyzed by table 2 it is found that strains tested is to the nymph of a locust have it is pathogenic, median lethal time between 3.55d~10.67d,
Bombys batryticatus rate is between 38.33%~93.33%.Wherein the median lethal time of Metarhizium Strains IPPMHBC-009 is 3.55d, stiff
Worm rate is 93.33%.Compared with bacterial strain 200614 etc., Metarhizium Strains IPPMHBC-009 has significant difference, shows it to Asia
Trolley locust has higher pathogenicity in continent.
Embodiment 3 measures Metarhizium anisopliae IPPMHBC-009 and is compounded with chemical pesticide to Oedaleus asiaticus B pathogenicity
Test sets 3 processing, and every processing includes 3 groups of the 3 age nymph of a locust, every group of 3 repetitions.1st processing low quality concentration
97.5% chlopyrifos (0.004,0.02,0.1ppm), the 2nd processing with green muscardine fungus IPPMHBC-009 processing (0.0064%,
0.032%, 0.16%), the 3rd processing mixed liquor of low concentration 97.5% chlopyrifos and green muscardine fungus IPPMHBC-009
(0.004ppm+0.0064%, 0.02ppm+0.032%, 0.1ppm+0.16%).
Processing method: the first processing group dilutes 97.5% chlopyrifos with acetone, obtains 0.004,0.02,0.1ppm；The
Two processing groups dilute green muscardine fungus IPPMHBC-009 conidia powder with wheat bran, obtain 0.0064%, 0.032%, 0.16% 3 it is dense
Degree；Third processing group compounds the first processing processing group with second processing group, obtains.
Test uses stomach toxicity method.It takes the bait formulation 0.2g prepared to be put into culture dish, is respectively put into together with culture dish each
It supports in worm basket, number.Each processing puts 15 for trying the 3 age nymphs of a locust, is placed in a feeding worm basket, feeds fresh sheep's hay daily.
Every processing is repeated 3 times.Daily observation 1 time, and observe and record.
The accumulative death of Oedaleus asiaticus B when chlopyrifos, green muscardine fungus IPPMHBC-009 and the mixing application of bacterium medicine is administered alone
Rate is as shown in Fig. 3, table 3.
Comparison of the 3 green muscardine fungus microbial inoculum of table to Oedaleus asiaticus B pathogenicity
As a result as shown in table 3, Fig. 3, after green muscardine fungus IPPMHBC-009 is used in mixed way with chlopyrifos, in quick-acting and desinsection
It all improves a lot in terms of rate, not only reduces the spore germination rate of green muscardine fungus to a certain extent, and greatly reduce pest
Immunity, be allowed to more easily by green muscardine fungus parasitism.Therefore, green muscardine fungus IPPMHBC-009 mixes application with chlopyrifos and can tie
The advantages of closing both sides, on the one hand can solve the lethal slow problem of fungus insecticide, on the other hand can also largely subtract
Of low pollution alleviates pest and develops drug resistance problem to chemical pesticide, to play synergistic purpose.
After green muscardine fungus IPPMHBC-009 of the invention is compounded with other chemical pesticides in addition to chlopyrifos, locust control
Effect is not simultaneously significant, or even the case where not rising anti-drop occurs.
Claims (4)
1. Metarhizium anisopliae IPPMHBC-009 composite bacteria agent, which is characterized in that the composite bacteria agent includes chlopyrifos and cockchafer
The deposit number of sub- green muscardine fungus IPPMHBC-009, the green muscardine fungus IPPMHBC-009 are CGMCC No.16000.
2. Metarhizium anisopliae IPPMHBC-009 composite bacteria agent according to claim 1, which is characterized in that described compound
The mass ratio of microbial inoculum Chlorpyrifos and green muscardine fungus IPPMHBC-009 are 1:1000~2000.
3. application of the Metarhizium anisopliae IPPMHBC-009 composite bacteria agent described in claim 1 in terms of preventing and treating locust.
4. a kind of method for preventing and treating locust, which is characterized in that the method includes to use the green deadlock of chafer described in claim 1
Bacterium IPPMHBC-009 composite bacteria agent prevents and treats the step of locust.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910186476.1A CN109769860A (en) | 2019-03-12 | 2019-03-12 | Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910186476.1A CN109769860A (en) | 2019-03-12 | 2019-03-12 | Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109769860A true CN109769860A (en) | 2019-05-21 |
Family
ID=66489084
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910186476.1A Pending CN109769860A (en) | 2019-03-12 | 2019-03-12 | Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109769860A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109337823A (en) * | 2018-10-11 | 2019-02-15 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBC-009 and its application |
-
2019
- 2019-03-12 CN CN201910186476.1A patent/CN109769860A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109337823A (en) * | 2018-10-11 | 2019-02-15 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBC-009 and its application |
Non-Patent Citations (2)
| Title |
|---|
| 张英才 等: "18种化学与绿僵菌相容性研究", 《中国生物防治学报》 * |
| 林超文 等: "《种草养畜：牧草栽培利用实用技术》", 30 September 2015, 四川科学技术出版社，第1版 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102864100B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN102838389B (en) | Fertilizer-and-drug double-effect bio-organic fertilizer for preventing and treating crop soil-borne diseases and manufacturing method of bio-organic fertilizer | |
| CN104286032B (en) | The preparation method of a kind of yellow basket bacterium spore powder, yellow basket bacterium wettable powder and preparation method thereof | |
| CN102851210A (en) | Livestock and poultry excrement microbial deodorizing agent, and preparation method and application thereof | |
| CN101463332A (en) | Antibiosis streptomycete | |
| CN102337237A (en) | Functional organic material decomposing agent and preparation method thereof | |
| CN105316243A (en) | Preparing method and application of composite biological agent for agricultural root-knot nematodes | |
| CN1316899C (en) | Mixed insecticide of green muscardine fungus and pyrethrum group and its preparation method | |
| CN101781565B (en) | Multifunctional biological agent containing Trichoderma pseudokiningii | |
| CN105039184A (en) | Beauveria bassiana strain having pathogenicity on cyclophragma undans and application thereof | |
| CN103749542B (en) | Bacillusamyloliquefaciens and validamycin compound biological bactericide and application thereof | |
| CN103749546B (en) | A kind of rose dark brown Isaria and beta-cypermethrin oil-suspending agent and application thereof | |
| CN109769861A (en) | Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application | |
| CN109730094A (en) | Metarhizium anisopliae IPPMHBHC-7 composite bacteria agent and its application | |
| CN109769859A (en) | Metarhizium anisopliae IPPMHBHC-7 composite bacteria agent and its application | |
| CN108913627B (en) | A kind of leavening of aflatoxin degradation, preparation method and application | |
| CN109769860A (en) | Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application | |
| CN102038004A (en) | Composite microorganism live bacterium preparation for killing nematodes | |
| CN105018395A (en) | Bacillus pumilus strain and application thereof in apple alternaria leaf spot prevention and control | |
| CN102648713A (en) | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein | |
| CN106260502A (en) | A kind of method prepared containing surface element feed additive | |
| CN105601358A (en) | Microbial organic fertilizer for preventing and controlling strawberry red core disease, and production method thereof | |
| CN106962408B (en) | A kind of gradually narrow Verticillium dahliae and the compounded pesticides of Osthole | |
| CN106083343A (en) | One seed manure medicine economic benefits and social benefits microbial manure and production method thereof | |
| CN105219650A (en) | A kind of tangerine green trichoderma bacterium and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190521 |