CN109337823A - Metarhizium anisopliae IPPMHBC-009 and its application - Google Patents

Metarhizium anisopliae IPPMHBC-009 and its application Download PDF

Info

Publication number
CN109337823A
CN109337823A CN201811181953.7A CN201811181953A CN109337823A CN 109337823 A CN109337823 A CN 109337823A CN 201811181953 A CN201811181953 A CN 201811181953A CN 109337823 A CN109337823 A CN 109337823A
Authority
CN
China
Prior art keywords
ippmhbc
locust
metarhizium anisopliae
metarhizium
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201811181953.7A
Other languages
Chinese (zh)
Other versions
CN109337823B (en
Inventor
王广君
郭隆隆
李贝贝
王晔青
涂雄兵
农向群
张泽华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Original Assignee
Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Plant Protection of Chinese Academy of Agricultural Sciences filed Critical Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Priority to CN201811181953.7A priority Critical patent/CN109337823B/en
Publication of CN109337823A publication Critical patent/CN109337823A/en
Application granted granted Critical
Publication of CN109337823B publication Critical patent/CN109337823B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to field of biotechnology, and in particular to a kind of Metarhizium anisopliae IPPMHBC-009 and its application.Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC-009, deposit number are CGMCC No.16000, have good LD50 time and bombys batryticatus rate for Oedaleus asiaticus B, Dasyhippus barbipes.

Description

Metarhizium anisopliae IPPMHBC-009 and its application
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of Metarhizium anisopliae IPPMHBC-009 and its application.
Background technique
Locust is the disastrous insect of Orthoptera locust section, and the plague of locusts frequently occurs in China, causes to China's agricultural huge Injury.Currently, China control grassland grasshopper harm means mainly use chemical prevention method, have quickly, efficiently, make With it is flexible the advantages that, still, as people increasingly pay attention to the sustainable development of environmental economy, biological locust elimination method is developed Come and applies in real work.Biology goes out locust method, especially green muscardine fungus, muscardine etc., has certain specificity, to people It raises harmless, while also having many advantages, such as that free from environmental pollution, noresidue, pest will not develop drug resistance.
Although disinsection fungal, which prevents and treats locust, has certain control efficiency, fungi is knocked down, killing off the insect pests acts on slowly, It needs just be worked after a period of time, is suitble to general generation time control and application, and in locust outbreak year part, or part When regional serious generation, disinsection fungal cannot quickly reduce insect density in the short time.Meanwhile in production practices and application process It was found that a degree of degeneration often occur in some bacterial strains, show as after several generations is manually cultivated, bacterium colony is unstable, aerial hyphae The features such as excessive growth, sporulation quantity are reduced, to influence control efficiency.
Summary of the invention
It is knocked down to solve fungi existing in the prior art, killing off the insect pests acts on the problems such as slow, present invention offer one Kind Metarhizium anisopliae IPPMHBC-009 and its application.
The purpose of the present invention is to provide a kind of Metarhizium anisopliae IPPMHBC-009.
A further object of the present invention is to provide the applications of Metarhizium anisopliae IPPMHBC-009.
A further object of the present invention is to provide the biological prevention and control agents containing Metarhizium anisopliae IPPMHBC-009.
A further object of the present invention is to provide the methods using Metarhizium anisopliae IPPMHBC-009 prevention and treatment locust.
Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC- of specific embodiment according to the present invention The deposit number CGMCC No.16000 of 009 bacterial strain, the bacterial strain were stored in China General Microbiological bacterium on 08 02nd, 2018 Kind collection, collection address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
Spore can be made in Metarhizium anisopliae IPPMHBC-009 by the biological prevention and control agent of specific embodiment according to the present invention Powder is directly applied, or is combined and is used with other chemicals.
The method of the prevention and treatment locust of specific embodiment according to the present invention, including sow or spray to field and contain green deadlock The Agrotechnical formulation of bacterium IPPMHBC-009.
Metarhizium anisopliae IPPMHBC-009 of the invention is 3.87d, bombys batryticatus rate to the median lethal time of Dasyhippus barbipes It is 91.67%, the median lethal time to Oedaleus asiaticus B is 3.55d, and bombys batryticatus rate is 93.33%, therefore, chafer of the invention Green muscardine fungus IPPMHBC-009 is with good application prospect.
Detailed description of the invention
Fig. 1 shows 9 kinds of green muscardine fungus to the cumulative mortality curve of Dasyhippus barbipes 15d;
Fig. 2 shows 9 kinds of green muscardine fungus to the cumulative mortality curve of Oedaleus asiaticus B 15d.
The deposit number CGMCC of Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC-009 bacterial strain No.16000 was stored in China General Microbiological Culture Collection Center, collection address: Beijing on 08 02nd, 2018 The Institute of Microorganism, Academia Sinica of institute of Chaoyang District North Star West Road 1.
Specific embodiment
Bacterium source of the present invention:
The present invention tests 9 kinds of green muscardine fungus (Metarhizium anisopliae) bacterial strain, numbers respectively are as follows:
①IPPBHC-5;
②IPPMHBC-009;
③IPPMHK-7;
④IPPM189;
⑤IPPM803;
⑥IPPM200614;
⑦IPPM2010-10;
⑧IPPB054;
⑨IPPMC384;
The source of test worm of the present invention:
1. Dasyhippus barbipes (Dasyhippus barbipes Fischer-Waldheim): 2~3 age nymphs of a locust, method of beating It is collected in In Xilingol League In Inner Mongolia white clouds coulomb locust area.
2. Oedaleus asiaticus B (Oedaleus decorus asiaticus B.Bienko): 2~3 age nymphs of a locust, the method for beating are adopted Combine in the In Xilingol League In Inner Mongolia west northern 7 kilometers of areas Chu Huang of black flag Bayan Gol.
The locust raising of acquisition feeds suitable fresh sheep's hay in the big frame in experiment station dedicated for supporting locust daily Blade.For guarantee test effect, test the previous day Nature enemy.
The screening of 1 green muscardine fungus IPPMHBC-009 of embodiment
Method is trapped from the soil of collection using greater wax moth and traps Metarhizium Strains.By with the scrap rubber of disinfection volume it is box-packed enter confession Soil sample is tried, every box is embedded to 1 greater wax moth, is repeated 3 times.Soil sample box is placed on 24~27 DEG C, after moisturizing traps 1 week, sorts out dead worm, After cultivating 7~10 days in 25~26 DEG C of constant temperature, relative humidity 100, aimed strain, strain isolation culture are separated from bombys batryticatus Base is PDAY.
The green muscardine fungus being separated to is inoculated on PDAY solid medium, culture to production spore, 0.1% tween water of conidia powder It suspends, vortex oscillator shaken well, adjustment spore concentration is 1 × 108A/mL.1mL spore suspension is taken to be inoculated into 100mL Cultural hypha base is produced, 28 DEG C, 200r/min shaking table shaken cultivation 3d to logarithmic phase are set.It is filtered to obtain mycelia with vacuum pump, takes 1g Mycelia liquid nitrogen grinding to powder extracts genomic DNA.Using genomic DNA as template, primer amplified is designed The ITS1-5.8SrRDNA-ITS2 sequence of IPPMHBC-009 bacterial strain.PCR product is separated through 1.5% agarose gel electrophoresis, is used Plastic recovery kit recycles purpose segment, is cloned into pGEM-T Easy Vector carrier, conversion to Trans-T1Phage In Resistant competent cell, transformant is screened by blue hickie on ampicillin plate, after PCR is detected, is surveyed Sequence.The sequence of measurement is subjected to BLAST analysis in GenBank, determines that bacterial strain is Metarhizium Strains, and name as IPPMHBC- 009。
Bioassay of the 2. green muscardine fungus deep blue 09 of embodiment to Dasyhippus barbipes nymph of a locust's pathogenicity
Above-mentioned 9 kinds of Metarhizium Strains are measured to the virulence effect of locust using stomach toxicity method.
0.2g muscardine spore powder is weighed first to be added in 2.0g wheat bran, adds 0.1ml edible oil, is sufficiently stirred It is even.Then it weighs the green muscardine fungus wheat bran bait formulation that 0.2g is prepared to be put into culture dish, is put into togerther cover glass plate together with culture dish Worm basket, number are supported, then is put into 20 3 age Dasyhippus barbipes nymphs of a locust of the same size in feeding worm basket.Blank control group wheat bran bait formulation In muscardine spore powder is not added.3 weights are arranged in totally 9 kinds of muscardine spore powder and a control CK, every kind of conidia powder for this experiment It is multiple, in being raised in insectary.Bait formulation was taken out in second day, feeds suitable fresh sheep's hay daily later, and investigate every basket of nymph of a locust Death condition, make a record respectively.
The dead nymph of a locust is sorted out, being lined in the culture dish of filter paper for reference numeral is put into, keeps certain humidity, be allowed to long Bombys batryticatus out.Investigate every kind of bacterium after the nymph of a locust to be tested is all dead treated the bombys batryticatus rate of the nymph of a locust, it is every in 15 days after combination processing The cumulative mortality and median lethal time (LT of day50) pick out to the preferable strain of locust lethal effect.
9 plants of Metarhizium Strains are shown in figure l, table 1 to the bioassay results of 3 age Dasyhippus barbipes nymph of a locust pathogenicities.
Comparison of the 19 kinds of green muscardine fungus of table to Dasyhippus barbipes pathogenicity
Analyzed by table 1 it is found that above-mentioned 9 kinds of strains testeds have to the nymph of a locust it is pathogenic, median lethal time 3.87d~ Between 8.83d, bombys batryticatus rate is between 43.33%~91.67%.Wherein the median lethal time of green muscardine fungus IPPMHBC-009 is 3.87d, bombys batryticatus rate is 91.67%, and the bacterial strains such as IPPM803, IPPM200614, IPPM2010-10 median lethal time is slightly long, stiff Worm rate is lower.Therefore, Metarhizium Strains IPPMHBC-009 exists significant compared with Metarhizium Strains IPPM803, IPPM200614 Difference shows that bacterial strain IPPMHBC-009 has higher pathogenicity to Dasyhippus barbipes.
Biometric of 3. green muscardine fungus of embodiment to Oedaleus asiaticus B pathogenicity
9 plants of bacterial strains are measured to the power of curing the disease of Oedaleus asiaticus B, method of the measuring method with embodiment 2.
9 plants of Metarhizium Strains are shown in Fig. 2, table 2 to the bioassay results of Oedaleus asiaticus B pathogenicity.
Comparison of the 29 plants of green muscardine fungus of table to Oedaleus asiaticus B pathogenicity
Analyzed by table 2 it is found that strains tested is to the nymph of a locust have it is pathogenic, median lethal time between 3.55d~10.67d, Bombys batryticatus rate is between 38.33%~93.33%, and wherein the median lethal time of green muscardine fungus IPPMHBC-009 is 3.55d, bombys batryticatus rate It is 93.33%.Therefore, Metarhizium Strains IPPMHBC-009 exists aobvious compared with Metarhizium Strains IPPM803, IPPM200614 Difference is write, shows that bacterial strain IPPMHBC-009 has higher pathogenicity to Oedaleus asiaticus B.
4. green muscardine fungus different strains growth rate of embodiment and sporulation quantity measurement
Different Metarhizium Strains spores is diluted to identical 2.5x108Spore/gram, take 10ul to be transferred to PDAY respectively On solid plate, plate is placed in 27 DEG C of incubators and is cultivated, since the 4th day, the colony diameter of measurement in every two days, as a result It is shown in Table 3.
The growth rate of the different Metarhizium Strains of table 3 compares
As shown in table 3, the growth rate of green muscardine fungus IPPMHBC-009 is apparently higher than control strain IPPM189 and other 4 Green muscardine fungus wild strain.
Different Metarhizium Strains are transferred on PDAY solid plate, continuously cultivated for 5 generations indoors, is often commissioned to train and supports to 15 days (about 10 days after production spore), measures its sporulation quantity, the results are shown in Table 4.
The sporulation quantity of the different Metarhizium Strains of table 4 compares
As shown in table 4, after culture mostly generation, IPPMHBC-009 still has preferable sporulation quantity, and it is steady to produce spore character It is fixed, it is not significantly different with control strain.

Claims (5)

  1. Metarhizium anisopliae 1. (Metarhizium anisopliae) IPPMHBC-009, which is characterized in that the chafer is green The deposit number of stiff bacterium IPPMHBC-009 is CGMCC No.16000.
  2. 2. Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC-009 according to claim 1 is anti- Application in locust elimination worm.
  3. 3. Metarhizium anisopliae (Metarhizium anisopliae) IPPMHBC-009 according to claim 2 is anti- Application in locust elimination worm, which is characterized in that the locust includes Oedaleus asiaticus B and/or Dasyhippus barbipes.
  4. 4. a kind of locust biocontrol agent, which is characterized in that the locust biocontrol agent includes cockchafer described in claim 1 Sub- green muscardine fungus (Metarhizium anisopliae) IPPMHBC-009.
  5. 5. a kind of method for preventing and treating locust, which is characterized in that comprising green using chafer described in claim 1 in the method The step of stiff bacterium (Metarhizium anisopliae) IPPMHBC-009 prevention and treatment locust.
CN201811181953.7A 2018-10-11 2018-10-11 Metarhizium anisopliae IPPMHBC-009 and application thereof Active CN109337823B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811181953.7A CN109337823B (en) 2018-10-11 2018-10-11 Metarhizium anisopliae IPPMHBC-009 and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811181953.7A CN109337823B (en) 2018-10-11 2018-10-11 Metarhizium anisopliae IPPMHBC-009 and application thereof

Publications (2)

Publication Number Publication Date
CN109337823A true CN109337823A (en) 2019-02-15
CN109337823B CN109337823B (en) 2020-05-22

Family

ID=65308995

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811181953.7A Active CN109337823B (en) 2018-10-11 2018-10-11 Metarhizium anisopliae IPPMHBC-009 and application thereof

Country Status (1)

Country Link
CN (1) CN109337823B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109769861A (en) * 2019-03-13 2019-05-21 中国农业科学院植物保护研究所 Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application
CN109769860A (en) * 2019-03-12 2019-05-21 中国农业科学院植物保护研究所 Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application
CN110876387A (en) * 2019-12-05 2020-03-13 中国农业科学院植物保护研究所 Compound bait for preventing and controlling locust and application thereof
CN112646735A (en) * 2021-01-21 2021-04-13 慕恩(广州)生物科技有限公司 Metarhizium anisopliae, microbial insecticide, preparation method and application
CN112970783A (en) * 2021-03-02 2021-06-18 中国农业科学院植物保护研究所 Composite bait agent of artemisia sieversiana crude extract and metarhizium anisopliae for preventing and treating locusts asiaticus and application of composite bait agent
CN114437936A (en) * 2021-12-02 2022-05-06 中国农业科学院植物保护研究所 Metarhizium anisopliae IPPMHB614 and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1542123A (en) * 2003-11-07 2004-11-03 重庆大学 Metarhizium anisopliae
EP3234097A1 (en) * 2014-12-16 2017-10-25 Bayer CropScience AG Method for prolonging the viability of fungal spores in liquid formulations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1542123A (en) * 2003-11-07 2004-11-03 重庆大学 Metarhizium anisopliae
EP3234097A1 (en) * 2014-12-16 2017-10-25 Bayer CropScience AG Method for prolonging the viability of fungal spores in liquid formulations

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CAO GUANGCHUN等: "Different Effects of Metarhizium anisopliae Strains IMI330189 and IBC200614 on Enzymes Activities and Hemocytes of Locusta migratoria L.", 《PLOS ONE》 *
麦迪•库尔曼等: "绿僵菌防治山地草原蝗虫的试验与示范", 《草食家畜》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109769860A (en) * 2019-03-12 2019-05-21 中国农业科学院植物保护研究所 Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application
CN109769861A (en) * 2019-03-13 2019-05-21 中国农业科学院植物保护研究所 Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application
CN110876387A (en) * 2019-12-05 2020-03-13 中国农业科学院植物保护研究所 Compound bait for preventing and controlling locust and application thereof
CN112646735A (en) * 2021-01-21 2021-04-13 慕恩(广州)生物科技有限公司 Metarhizium anisopliae, microbial insecticide, preparation method and application
CN112970783A (en) * 2021-03-02 2021-06-18 中国农业科学院植物保护研究所 Composite bait agent of artemisia sieversiana crude extract and metarhizium anisopliae for preventing and treating locusts asiaticus and application of composite bait agent
CN114437936A (en) * 2021-12-02 2022-05-06 中国农业科学院植物保护研究所 Metarhizium anisopliae IPPMHB614 and application thereof
CN114437936B (en) * 2021-12-02 2023-06-20 中国农业科学院植物保护研究所 Metarhizium anisopliae IPPMHB614 and application thereof

Also Published As

Publication number Publication date
CN109337823B (en) 2020-05-22

Similar Documents

Publication Publication Date Title
CN109337823A (en) Metarhizium anisopliae IPPMHBC-009 and its application
CN109401981A (en) Metarhizium anisopliae IPPMHBHC-7 and its application
Pereira et al. Endophytic fungi from Musa acuminata and their reintroduction into axenic plants
CN105219654B (en) One plant of aspergillus flavus strain for not producing aflatoxin and its application in aflatoxin pollution of peanuts biological control
Rombach et al. Production of Beauveria bassiana [Deuteromycotina: Hyphomycetes] in different liquid media and subsequent conidiation of dry mycelium
CN104087510A (en) Beauveria bassiana and application thereof
CN104073439A (en) Beauveria bassiana(Bals.-Criv.)Vuill and application thereof
CN108315267A (en) Short close trichoderma GSAAMLSHU-1 and its application
Shah et al. Method to immobilize the aphid-pathogenic fungus Erynia neoaphidis in an alginate matrix for biocontrol
Tansey Isolation of thermophilic fungi from alligator nesting material
CN103725628B (en) The Endophyte of walnut bacterial strain of the one anti-walnut Peptic Ulcers of strain and application thereof
CN107760630B (en) Bacillus methylotrophicus B18, microbial inoculum and application thereof
CN109971658B (en) Aspergillus fungus strain and application thereof
Abdullaev et al. Role of Beauveria tenella (Del) Siem BD-85 in the control of the populations of termites from the genus Anacanthotermes Jacobson.
Bhattacharyya et al. Fungal endophytes associated with the ethnomedicinal plant Meyna spinosa Roxb
CN108085275B (en) Enterobacter ludwigii for inhibiting pathogenic fungi of white muscardine silkworm and application thereof
CN107254414B (en) Biocontrol strain AW2017 for preventing and treating rice root-knot nematode and application thereof
Gardner et al. Tolypocladium cylindrosporum (Deuteromycotina: Moniliales), a fungal pathogen of the mosquito Aedes australis: II. Methods of spore propagation and storage
CN104073441A (en) Metarhizium acridum and application thereof
Ward Jr et al. Thermophilic fungi of some central South Carolina forest soils
CN103704074B (en) Green muscardine fungus sprays the method for ground, compost place control white star brevitarsis larva
CN109055236B (en) Isaria pinicola WSWM1171 and application thereof in prevention and control of potato corm moth pupae
Bhuyan et al. Phyllosphere microflora of Muga silkworm host plant Persea bombycina Kost (Som) leaves in Jorhat District of Assam, India
CN105176835A (en) Beauveria bassiana(balsamo) vuillemin strain for controlling eucalyptus locates moths, and screening and identifying method of beauveria bassiana(balsamo) vuillemin strain
CN107325973A (en) One plant there is High pathogenicity to hazel stinkbug muscardine bacterial strain and its application

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant