CN109769820A - The purposes of pink neck Cerambycid beetles attractant and (2R, 5R)-theaspirane - Google Patents
The purposes of pink neck Cerambycid beetles attractant and (2R, 5R)-theaspirane Download PDFInfo
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- CN109769820A CN109769820A CN201910178907.XA CN201910178907A CN109769820A CN 109769820 A CN109769820 A CN 109769820A CN 201910178907 A CN201910178907 A CN 201910178907A CN 109769820 A CN109769820 A CN 109769820A
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- attractant
- theaspirane
- pink neck
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- longicorn
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Abstract
The invention discloses the purposes that a kind of pink neck Cerambycid beetles attractant and (2R, 5R)-theaspirane are used to prepare pink neck longicorn attractant.Pink neck Cerambycid beetles attractant of the present invention, the attractant are the hexane solution of (2R, 5R)-theaspirane of 0.15 ~ 1.5 μ g/ μ L of concentration；Or, the attractant is the hexane solution of the theaspirane of 0.5 ~ 5 μ g/ μ L of concentration.Present invention discover that (2R, 5R)-Theaspirane is female to pink neck longicorn, male imago has attracting action, the problems such as human cost caused by attractant effective solution is made into due to manual catching and killing adult, punching injection or medicine puree smear prevention and treatment larva spends larger, influence fruit quality and environmental pollution, can provide practical nuisanceless monitoring, Prevention Technique means for the generation phase of monitoring inforests adult, occurrence quantity or extensive trapping.
Description
Technical field
The present invention relates to the prevention and treatment of longicorn class pest, specifically pink neck Cerambycid beetles attractant and (2R, 5R)-tea spiral shell
The purposes of alkane.
Background technique
Pink neck longicorn (Aromia bungiiFald) belong to coleoptera (Coleoptera) Cerambycidae
It (Cerambycidae), is the important trunk borer of many fruit trees, forest and ornamental trees.Since its harm is hidden, prevention and treatment is tired
Difficulty often results in destructive disaster.In China in addition to the Tibet of Xinjiang, pink neck longicorn is distributed, in South Korea, Vietnam, Russia sieve
This and also have been reported that elsewhere.There is invasion in Japan and several European countries discovery including Italy and Germany recently
Phenomenon.And be also trapped in Britain, the U.S. and Australia, it has attracted worldwide attention.
Current control method mainly takes manual catching and killing and chemical pesticide control.Wherein, it punches injection or medicine puree applies
The method for smearing prevention and treatment larva easily causes potentially to influence on fruit quality；Manual catching and killing has certain effect, but due to its adult
Sprout wings irregular, therefore spends human cost larger.Carrying out control of insect and monitoring using botanical attractant has specificity
By force, high-efficient, the advantages that being not easy to produce resistance, is pollution-free, therefore, suitable and there is effect attractive activity to pink neck longicorn
Substance will have very big help to the prevention and treatment of pink neck longicorn.
Summary of the invention
The object of the present invention is to provide a kind of substance --- (2R, 5R)-tea spiral shells to pink neck longicorn with attracting action
Alkane (i.e. (2R, 5R)-Theaspirane), while a kind of attractant for containing (2R, 5R)-theaspirane being provided, to solve existing peach
Effectively lure means due to lacking during the prevention and treatment of red neck longicorn, so as to cause pink neck longicorn manual catching and killing higher cost,
There is adverse effect to fruit quality and ecological environment in time-consuming and laborious problem and chemical pesticide control.
An object of the present invention is achieved in that
A kind of pink neck Cerambycid beetles attractant contains (2R, 5R)-theaspirane in the attractant.
Further, pink neck Cerambycid beetles attractant of the present invention, the attractant are 0.15 ~ 1.5 μ g/ of concentration
The hexane solution of (2R, 5R)-theaspirane of μ L；Or,
The attractant is the hexane solution of the theaspirane of 0.5 ~ 5 μ g/ μ L of concentration.
The second object of the present invention is to what is be achieved:
A kind of (2R, 5R)-theaspirane is used to prepare the purposes of pink neck longicorn attractant.
Using botanical attractant carry out control of insect and monitoring have high specificity, it is high-efficient, be not easy to produce resistance, nothing
The advantages that pollution, present invention discover that (2R, 5R)-Theaspirane has female to pink neck longicorn, male imago attracting action,
Being made into attractant effective solution manual catching and killing spends human cost larger, punches injection or medicine puree smears prevention and treatment larva
Easily fruit quality is caused the problems such as potentially influencing, and can be the generation phase of monitoring inforests adult, occurrence quantity or big rule
Mould trapping provides practical nuisanceless monitoring, Prevention Technique means.
Detailed description of the invention
Fig. 1 is GC-EAD response diagram of the pink neck longicorn male worm feeler to peach fruit volatile matter.
Fig. 2 is GC-EAD response diagram of the pink neck longicorn female adult feeler to peach fruit volatile matter.
Fig. 3 is the total ion current figure of peach fruit volatile matter.
Fig. 4 is EAG response diagram of the pink neck longicorn feeler of male to the Theaspirane of various dose.
Fig. 5 is EAG response diagram of the pink neck longicorn feeler of female to the Theaspirane of various dose.
Specific embodiment
More detailed elaboration is carried out to technical solution of the present invention below by specific embodiment.
Embodiment 1-3
With n-hexane (Ke Miou, chromatographically pure) for solvent, with theaspirane (standard items) for solute, prepares theaspirane concentration and be followed successively by
The theaspirane solution of 50 μ g/ μ L, 5 μ g/ μ L, 0.5 μ g/ μ L (respectively embodiment 1/2/3), according to (2R, 5R)-tea in theaspirane
The content of spirane calculates, and the concentration of (2R, 5R)-theaspirane is followed successively by 15 μ g/ μ L, 1.5 μ g/ μ L, 0.15 μ g/ μ L.
Selected insect source:
It is that parasitic larva from aggrieved peach log waits for that it is sprouted wings as experiment worm sources that tentaculum electric potential, which tests adult used,；It is Y-Shaped
Olfactometer, which tests adult used, directly to be acquired in peach garden in the emergence peak period of pink neck longicorn.
One, tentaculum electric potential is tested
Experiment 1: utilizing solid phase microextraction-gas-chromatography-tentaculum electric potential technology, identifies in peach fruit to the pink neck longicorn feeler of male
There is the substance of electrophysiologic activity, as a result as shown in Figure 1.
Experiment 2: it utilizes in solid phase microextraction-gas-chromatography-tentaculum electric potential technical appraisement peach fruit to the pink neck longicorn of female
Feeler has the substance of electrophysiologic activity, as a result as shown in Figure 2.
Experiment 3: there is electro physiology living pink neck longicorn feeler by solid phase microextraction-Gas chromatographyMass spectrometry
The substance of property identified, as a result as shown in Figure 3.
Experiment 4: the minimum of electrophysiologic response is generated to the pink longicorn of male using tentaculum electric potential instrument test Theaspirane
Dosage, as a result as shown in Figure 4.
Experiment 5: the minimum of electrophysiologic response is generated to the pink longicorn of female using tentaculum electric potential instrument test Theaspirane
Dosage, as a result as shown in Figure 5.
By Fig. 1, Fig. 2 and Fig. 3 is combined it is recognised that the pink neck longicorn of male and female has electro physiology living (2R, 5R)-theaspirane
Property.
(2R, 5R)-theaspirane has the function of luring pink neck longicorn it can be seen from identifying and testing above, (2R,
5R)-theaspirane hexane solution has extraordinary attracting action to pink neck longicorn in 1.5 μ g/ μ L concentration, can be used for
Prepare pink neck longicorn attractant.
The analysis of embodiment data:
Two, behavior reaction is tested
Pink neck Cerambycid beetles are tested to the behavior reaction of Theaspirane using Y-Shaped olfactometer.
Test environment: Y-Shaped pipe is made of glass, principal arm pipe range 40cm, two side arms a length of 30cm, internal diameter 5.5cm, side arm
Between angle be 75 °.There is the trap ball being made into round flask on two sidewalls arm top respectively, and two ball volumes are 2000
ML, long 35cm are fallen when climbing to herein for examination adult, and anti-non-return is climbed.Two balls are separately connected container, flow equipped with odor source
Meter, active carbon, the air generator for loading active carbon and the silica gel that changes colour.All devices are all made of the connection of Teflon pipe.Y type
Olfactometer is placed in darkroom, and for two points of arms towards lamp, illuminance is about 4.80 × 106lux.Test the odor source of arm
It is with the n-compound hexane solution of the Theaspirane of 10 μ L various concentrations, control arm is 10 μ L n-hexanes.
Test condition: we test the reaction of male or female individuals to Theaspirane.Pure air flows through each
The flow for dividing arm is 0.4 L/min.The principal arm end that test worm single head is introduced to Y-Shaped pipe when test, if test worm is creeped more in 5 min
The half of excessive arm falls into one's trap, that is, is considered as and makes a choice.It does not make a choice in 10min, then abandons the test worm, be not counted in
Statistical analysis.Every test worm is only tested once, and every group of test, which at least needs 30, reaction adult.5 test worms of every test, will test
Arm and control arm exchange, to eliminate influence of the position effect to performance testing.Every time before experiment, with dehydrated alcohol by Y-Shaped smell
The all parts of instrument clean, and distilled water flushing is clean, high hot-air seasoning.Behaviour bioassay room is maintained at 20 ~ 25 DEG C, relative humidity
45%~50%.Need for adult single head to be placed in front of test in the PE box of 18 × 10.5 × 18cm it is hungry for 24 hours (26 ± 0.5 DEG C, it is relatively wet
Degree 55% ± 5%).
Behavior selection analysis is all made of the progress of 21 software of SPSS, relevant using bivariate in non-parametric test
Wilcoxon method, the result that more pink neck longicorn female, male insect select the Theaspirane of various concentration.
Test 1: the odor source for testing arm is that (n-hexane of the theaspirane of 50 μ g/ μ L is molten for attractant prepared by embodiment 1
Liquid) 10 μ L do the experiment of Y-Shaped olfactometer, and control arm is 10 μ L n-hexanes.
The result shows that: the pink neck Cerambycid beetles of 32 females are used for Y-Shaped olfactometer experiment, wherein 10 have selected reality
Test arm, 22 selected control arm (Z=-2.121,P< 0.05).The pink neck Cerambycid beetles of 39 males are used for Y-Shaped smell
Instrument experiment, wherein 16 males have selected experiment arm, 23 selected control arm (Z=-1.121,P> 0.05).
Test 2: the odor source for testing arm is that (n-hexane of the theaspirane of 5 μ g/ μ L is molten for attractant prepared by embodiment 2
Liquid) 10 μ L do the experiment of Y-Shaped olfactometer, and control arm is 10 μ L n-hexanes.
The result shows that: the pink neck Cerambycid beetles of 33 females are used for Y-Shaped olfactometer experiment, wherein 23 have selected reality
Test arm, 10 selected control arm (Z=-2.263,P< 0.05).The pink neck Cerambycid beetles of 32 males are used for Y-Shaped smell
Instrument experiment, wherein 18 males have selected experiment arm, 14 selected control arm (Z=-0.707,P> 0.05).
Test 3: the odor source for testing arm is the (n-hexane of the theaspirane of 0.5 μ g/ μ L of attractant prepared by embodiment 3
Solution) 10 μ L do the experiment of Y-Shaped olfactometer, and control arm is 10 μ L n-hexanes.
The result shows that: the pink neck Cerambycid beetles of 47 females are used for Y-Shaped olfactometer experiment, wherein 33 have selected reality
Test arm, 14 selected control arm (Z=-2.771,P< 0.05).The pink neck Cerambycid beetles of 56 males are used for Y-Shaped smell
Instrument experiment, wherein 38 males have selected experiment arm, 18 selected control arm (Z=-2.405,P <0.05)。
In summary test result and Fig. 4/Fig. 5 can be seen that the pink neck Cerambycid beetles attractant that the present invention is identified,
Within the scope of 0.5 ~ 5 μ g/ μ L, low concentration is relatively good to the attractant effect of pink neck longicorn female and male, therefore in field
The theaspirane hexane solution of 0.5 μ g/ μ L is preferably used to do lure when trapping.
Claims (3)
1. a kind of pink neck Cerambycid beetles attractant, which is characterized in that contain (2R, 5R)-theaspirane in the attractant.
2. pink neck Cerambycid beetles attractant according to claim 1, which is characterized in that the attractant is concentration 0.15
The hexane solution of (2R, 5R)-theaspirane of ~ 1.5 μ g/ μ L；Or,
The attractant is the hexane solution of the theaspirane of 0.5 ~ 5 μ g/ μ L of concentration.
3. the purposes that a kind of (2R, 5R)-theaspirane is used to prepare pink neck longicorn attractant.
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| CN201910178907.XA CN109769820B (en) | 2019-03-11 | 2019-03-11 | Adult peach-red-neck longicorn attractant and application of (2R,5R) -theaspirane |
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| CN201910178907.XA CN109769820B (en) | 2019-03-11 | 2019-03-11 | Adult peach-red-neck longicorn attractant and application of (2R,5R) -theaspirane |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112293418A (en) * | 2020-11-09 | 2021-02-02 | 中国林业科学研究院华北林业实验中心 | Peach red neck longicorn attractant, slow release bottle and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101072501A (en) * | 2004-10-13 | 2007-11-14 | Efal化学工业有限公司 | Agents for control of codling moth in fruit orchards |
| WO2018142145A1 (en) * | 2017-02-03 | 2018-08-09 | Rothamsted Research Ltd | Pest attractant compositions comprising theaspirane |
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2019
- 2019-03-11 CN CN201910178907.XA patent/CN109769820B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101072501A (en) * | 2004-10-13 | 2007-11-14 | Efal化学工业有限公司 | Agents for control of codling moth in fruit orchards |
| WO2018142145A1 (en) * | 2017-02-03 | 2018-08-09 | Rothamsted Research Ltd | Pest attractant compositions comprising theaspirane |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112293418A (en) * | 2020-11-09 | 2021-02-02 | 中国林业科学研究院华北林业实验中心 | Peach red neck longicorn attractant, slow release bottle and preparation method thereof |
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Effective date of registration: 20230807 Address after: No. 388, Nongda Third Branch, Baoshuo Road, Wuyao Township, Lianchi District, Baoding City, Hebei Province, 071000 Patentee after: Baoding Wojia Agricultural Technology Co.,Ltd. Address before: 071002 Hebei University, 54 East 180 Road, Hebei, Baoding Patentee before: HEBEI University |
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