CN109762857A - A kind of technique preparing xanthan gum - Google Patents
A kind of technique preparing xanthan gum Download PDFInfo
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- CN109762857A CN109762857A CN201711097912.5A CN201711097912A CN109762857A CN 109762857 A CN109762857 A CN 109762857A CN 201711097912 A CN201711097912 A CN 201711097912A CN 109762857 A CN109762857 A CN 109762857A
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- xanthan gum
- filtrate
- thallus
- ultrasonic treatment
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Abstract
The invention belongs to fermentation technical fields, disclose a kind of technique for preparing xanthan gum comprising following steps: step 1) will produce cultivates in glue Xanthomonas campestris seed liquor access fermentation medium, 30 DEG C of temperature, fermented incubation time 60 hours, obtains fermentation liquid；Step 2 is separated xanthan gum feed liquid and wet thallus using high-speed dish piece seperator；Wet thallus is ultrasonically treated by step 3), and hydrogen peroxide and carbide slag are added into the wet thallus after ultrasonic treatment, and stirring is then added in the Dialysis culture base of 3-5 times of weight, cultivates 6-8h, and micro-filtrate membrane filtration collects thallus and filtrate；Step 4) merges filtrate obtained by the gained xanthan gum feed liquid and step 3) of step 2, for extracting xanthan gum.The yield of xanthan gum of present invention process strong operability, preparation greatly improves.
Description
Technical field
The invention belongs to fermentation technical fields, and in particular to a kind of technique for preparing xanthan gum.
Background technique
Xanthan gum is also known as xanthan gum, is a kind of widely used micro- life produced as raw material through microbial fermentation using starch sugar
Object polysaccharide.Xanthan gum good water solubility sufficiently forms high viscosity solution after hydration, be the current thickening of collection in the world, suspend, emulsification,
It is stable at the biogum got in integrated best performance；It can be used as emulsifier, stabilizer, gelling thickener, size, film molding
Agent etc.；It is widely used in the fields such as food, medicine, chemical industry, petroleum.
It is fermented using Xanthomonas campestris to prepare xanthan gum, is fermented by bacterial strain at present, product secretion is extracellular, to hair
Zymotic fluid carries out processing and extracts acquisition purpose product.The critical function of cell membrane first is that permselectivity by selectively leading to
Thoroughly, cell can accept or reject, and retain or be discharged certain substance.Manually change permeability of cell membranes, thus it is possible to vary raw material
Input or metabolite rate of discharge, so that metabolite is in the accumulation of extracellular (in culture medium), make metabolism towards
It is desirable to direction progress, be realize metabolism artificial regulatory one of important method.Therefore, cell membrane leads to metabolite
Permeability fermentation industry be a particularly significant problem it is widely used at present be chemical method, the disadvantage is that low efficiency,
Intracellular organic matter release rate is low, and the processing time is long, and reagent dosage is big, at high cost, while post-processing also inconvenience, and due to chemical reagent
Toxicity, further separation when need to dialysis the methods of remove.After fermentation, degerming is gone by way of filtering or being centrifuged
Body, then fermentation liquid is purified to obtain product；However, discarded thallus still has certain acid producing ability, how to useless
Abandoning thallus again is our technical issues that need to address with the high yield of xanthan gum of benefit.
Summary of the invention
For overcome the deficiencies in the prior art, using the secondary generation xanthan gum of discarded thallus, the present invention provides a kind of systems
The technique of standby xanthan gum.
The present invention is realized by following scheme:
A kind of technique preparing xanthan gum comprising following steps:
Step 1) will produce cultivates in glue Xanthomonas campestris seed liquor access fermentation medium, and 30 DEG C of temperature, fermented incubation time 60 is small
When, obtain fermentation liquid；
Step 2 is separated xanthan gum feed liquid and wet thallus using high-speed dish piece seperator；
Wet thallus is ultrasonically treated by step 3), and hydrogen peroxide and carbide slag are added into the wet thallus after ultrasonic treatment,
Stirring, is then added in the Dialysis culture base of 3-5 times of weight, cultivates 6-8h, and micro-filtrate membrane filtration collects thallus and filtrate；
Step 4) merges filtrate obtained by the gained xanthan gum feed liquid and step 3) of step 2, for extracting xanthan gum.
Preferably,
It is 20% by adjusting speed of agitator and ventilatory capacity holding dissolved oxygen level, by flowing automatically during the fermented and cultured
Add glucose solution by residual sugar control not less than 1.5%.
Preferably,
The preparation method of the fermentation medium includes the following steps: to take glucose 9%, soybean protein according to weight percent
0.8%, corn pulp 2.5%, epsom salt 0.3%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, ferrous sulfate heptahydrate
0.001%, VB10.00001%, remaining is water, is stirred evenly, and pH6.5 is adjusted；
Preferably,
The revolving speed of the high-speed dish piece machine is 4000rpm, time 5min.
Preferably,
The parameter of the ultrasonic treatment: frequency power 400-600w, ultrasonic action time are each 3s, and interval time is
4s, the total time of ultrasonic treatment are 60-90s.
Preferably,
In the step 3), 1wt% hydrogen peroxide and 3wt% carbide slag are added into the wet thallus after ultrasonic treatment,
100rpm stirs 30-60min, is then added in the Dialysis culture base of 3-5 times of weight, 30 DEG C of cultivation temperature, 100rpm stirring
6-8h is cultivated, micro-filtrate membrane filtration collects thallus and filtrate.
Preferably,
The Dialysis culture base are as follows: potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate heptahydrate 0.02%, seven water sulfuric acid
Magnesium 0.02%, adjustment pH are 6.8, and the above are mass percents.
The xanthan gum prepared according to above-mentioned technique is also claimed in the present invention.
The beneficial effect that the present invention obtains mainly includes but is not limited to the following aspects:
The present invention is improved for microbial fermentation technology, avoids xanthan gum concentration buildup to causing the feedback inhibition to adjust,
Secondary treatment is carried out for discarded thallus, permeability of cell membranes is increased, improves the acid producing ability of bacterial strain；
The cavitation of low frequency ultrasound can lead to the non-thermal biological effect of cell, make cell membrane partial fracture in short-term, to change
Attenuate the permeability of cytoplasmic membrane, is discharged into intracellular organic matter extracellularly；
Tourmaline can discharge anion automatically, and anion has stronger oxidisability, and DC static also persistently occurs, and discharge mine
Substance and microelement, play a driving role to microbial reproduction；By cheap natural minerals tourmaline and hydrogen peroxide with it is micro-
Biology mixing, discharges oxygen and negative ion element is that microorganism provides breeding and metabolic conditions；
The present invention cultivates bacterial strain using Dialysis culture base, and feedback inhibition adjusting substantially reduces, and produces sour efficiency and improves, and
Subsequent residual sugar is few, not will cause bacterial strain stick together flocculation it is agglomerating, have and be separated by filtration using subsequent film；
Use centrifugal separation technology first after fermentation, removal generates the substance of inhibiting effect, since inhibiting effect releases, carefully
Born of the same parents' enzyme activity is restored, and bacterial strain produces glue ability and is restored.
Figure of description
Fig. 1: the influence of ultrasound intensity, time to yield of xanthan gum.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, specifically real below in conjunction with the application
Example is applied, the present invention is more clearly and completely described, it is clear that described embodiment is only that the application a part is real
Example is applied, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making creation
Property labour under the premise of every other embodiment obtained, should fall within the scope of the present invention.
Embodiment 1
A kind of technique preparing xanthan gum comprising following steps:
Glue Xanthomonas campestris seed liquor (1 × 10 will be produced8Cfu/ml) according in the inoculum concentration access fermentation medium of 5% (volume ratio)
Culture, fermented incubation time 60 hours, obtains fermentation liquid by 30 DEG C of temperature；During fermented and cultured, by adjust speed of agitator with
It is 20% that ventilatory capacity, which keeps dissolved oxygen level, controls residual sugar by auto-feeding glucose solution and is being not less than 1.5%,
The preparation method of the fermentation medium includes the following steps: to take glucose 9%, soybean protein according to weight percent
0.8%, corn pulp 2.5%, epsom salt 0.3%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, ferrous sulfate heptahydrate
0.001%, VB10.00001%, remaining is water, is stirred evenly, and pH6.5 is adjusted；
Xanthan gum feed liquid and wet thallus are separated using high-speed dish piece seperator；The revolving speed of high-speed dish piece machine is 4000rpm, time
For 5min；Wet thallus is ultrasonically treated, the parameter of ultrasonic treatment: frequency power 400w, ultrasonic action time are every
Secondary 3s, interval time 4s, the total time of ultrasonic treatment are 90s, and into the wet thallus after ultrasonic treatment, addition 1wt% is bis-
Oxygen water and 3wt% carbide slag, 100rpm stir 30min, are then added in the Dialysis culture base of 5 times of weight, cultivation temperature 30
DEG C, 100rpm stir culture 8h, micro-filtrate membrane filtration collects thallus and filtrate；The Dialysis culture base is (mass percent): phosphorus
Acid dihydride potassium 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate heptahydrate 0.02%, epsom salt 0.02%, adjustment pH are 6.8；
Xanthan gum feed liquid and filtrate are merged, for extracting xanthan gum.
Embodiment 2
A kind of technique preparing xanthan gum comprising following steps:
Glue Xanthomonas campestris seed liquor (1 × 10 will be produced8Cfu/ml) according in the inoculum concentration access fermentation medium of 5% (volume ratio)
Culture, fermented incubation time 60 hours, obtains fermentation liquid by 30 DEG C of temperature；During fermented and cultured, by adjust speed of agitator with
It is 20% that ventilatory capacity, which keeps dissolved oxygen level, controls residual sugar by auto-feeding glucose solution and is being not less than 1.5%,
The preparation method of the fermentation medium includes the following steps: to take glucose 9%, soybean protein according to weight percent
0.8%, corn pulp 2.5%, epsom salt 0.3%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, ferrous sulfate heptahydrate
0.001%, VB10.00001%, remaining is water, is stirred evenly, and pH6.5 is adjusted；
Xanthan gum feed liquid and wet thallus are separated using high-speed dish piece seperator；The revolving speed of high-speed dish piece machine is 4000rpm, time
For 5min；Wet thallus is ultrasonically treated, the parameter of ultrasonic treatment: frequency power 600w, ultrasonic action time are every
Secondary 3s, interval time 4s, the total time of ultrasonic treatment are 60s, and into the wet thallus after ultrasonic treatment, addition 1wt% is bis-
Oxygen water and 3wt% carbide slag, 100rpm stir 30min, are then added in the Dialysis culture base of 5 times of weight, cultivation temperature 30
DEG C, 100rpm stir culture 6-8h, micro-filtrate membrane filtration collects thallus and filtrate；The Dialysis culture base is (mass percent):
Potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate heptahydrate 0.02%, epsom salt 0.02%, adjustment pH are 6.8；
Xanthan gum feed liquid and filtrate are merged, for extracting xanthan gum.
Embodiment 3
A kind of technique preparing xanthan gum comprising following steps:
Glue Xanthomonas campestris seed liquor (1 × 10 will be produced8Cfu/ml) according in the inoculum concentration access fermentation medium of 5% (volume ratio)
Culture, fermented incubation time 60 hours, obtains fermentation liquid by 30 DEG C of temperature；During fermented and cultured, by adjust speed of agitator with
It is 20% that ventilatory capacity, which keeps dissolved oxygen level, controls residual sugar by auto-feeding glucose solution and is being not less than 1.5%,
The preparation method of the fermentation medium includes the following steps: to take glucose 9%, soybean protein according to weight percent
0.8%, corn pulp 2.5%, epsom salt 0.3%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, ferrous sulfate heptahydrate
0.001%, VB10.00001%, remaining is water, is stirred evenly, and pH6.5 is adjusted；
Xanthan gum feed liquid and wet thallus are separated using high-speed dish piece seperator；The revolving speed of high-speed dish piece machine is 4000rpm, time
For 5min；Wet thallus is ultrasonically treated, the parameter of ultrasonic treatment: frequency power 500w, ultrasonic action time are every
Secondary 3s, interval time 4s, the total time of ultrasonic treatment are 80s, and into the wet thallus after ultrasonic treatment, addition 1wt% is bis-
Oxygen water and 3wt% carbide slag, 100rpm stir 50min, are then added in the Dialysis culture base of 4 times of weight, cultivation temperature 30
DEG C, 100rpm stir culture 7h, micro-filtrate membrane filtration collects thallus and filtrate；The Dialysis culture base is (mass percent): phosphorus
Acid dihydride potassium 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate heptahydrate 0.02%, epsom salt 0.02%, adjustment pH are 6.8；
Xanthan gum feed liquid and filtrate are merged, for extracting xanthan gum.
Comparative example 1
A kind of technique preparing xanthan gum comprising following steps:
Glue Xanthomonas campestris seed liquor (1 × 10 will be produced8Cfu/ml) according in the inoculum concentration access fermentation medium of 5% (volume ratio)
Culture, fermented incubation time 68 hours, obtains fermentation liquid by 30 DEG C of temperature；During fermented and cultured, by adjust speed of agitator with
It is 20% that ventilatory capacity, which keeps dissolved oxygen level, controls residual sugar by auto-feeding glucose solution and is being not less than 1.5%；Utilize height
Fast disc separator separates xanthan gum feed liquid and wet thallus.
Comparative example 2
A kind of technique preparing xanthan gum comprising following steps:
Glue Xanthomonas campestris seed liquor (1 × 10 will be produced8Cfu/ml) according in the inoculum concentration access fermentation medium of 5% (volume ratio)
Culture, fermented incubation time 60 hours, obtains fermentation liquid by 30 DEG C of temperature；During fermented and cultured, by adjust speed of agitator with
It is 20% that ventilatory capacity, which keeps dissolved oxygen level, controls residual sugar by auto-feeding glucose solution and is being not less than 1.5%,
Xanthan gum feed liquid and wet thallus are separated using high-speed dish piece seperator；The revolving speed of high-speed dish piece machine is 4000rpm, time
For 5min；Wet thallus is ultrasonically treated, the parameter of ultrasonic treatment: frequency power 400w, ultrasonic action time are every
Secondary 3s, interval time 4s, the total time of ultrasonic treatment are 90s, and the wet thallus after ultrasonic treatment is added to 5 times of weight
Dialysis culture base in, 30 DEG C of cultivation temperature, 100rpm stir culture 8h, micro-filtrate membrane filtration collects thallus and filtrate；It is described
Analysing culture medium is (mass percent): potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate heptahydrate 0.02%, seven water sulphur
Sour magnesium 0.02%, adjustment pH are 6.8；
Xanthan gum feed liquid and filtrate are merged, for extracting xanthan gum.
Embodiment 4
Influence of each factor to Fungal biodiversity, production xanthan glue amount
1, comparative example 1-2 and the Fungal biodiversity of embodiment 1 and production acid amount comparative test, are the fermentation of 100L with fermentation medium
Scale compares, and calculates total Fungal biodiversity and total yield acid amount.Specifically it is shown in Table 1:
Table 1
| Group | Fungal biodiversity kg | Yield of xanthan gum kg |
| Embodiment 1 | 8.39 | 2.76 |
| Comparative example 1 | 6.78 | 2.11 |
| Comparative example 2 | 7.62 | 2.48 |
As shown in table 1, compared with comparative example 1-2, the embodiment of the present invention 1 finally obtains Fungal biodiversity and yield of xanthan gum most
It is high, wherein by calculating, the quality of Fungal biodiversity is the 1.24 of comparative example 1-2,1.1 times respectively, and yield of xanthan gum is respectively
1.31,1.2 times of comparative example 1-2.
2, the influence of ultrasound intensity, time to yield of xanthan gum:
It is 200w, 400w, 600w that ultrasonic power, which is set separately, and tetra- groups of 800w, ultrasonic action time is each 3s, interval
Time is 4s, is set as 30s, 60s, 90s, 120s total time.It is compared with fermentation medium for the fermentation-scale of 100L,
His mode of operation is referring to embodiment 2.As shown in Figure 1, yield of xanthan gum is declined with the increase of ultrasound intensity, prompted
Strong ultrasonic wave can produce glue vigor to bacterial strain and have an impact, and therefore, control is 400-600w in ultrasound intensity, and total sonication time is
The ultrasonic treatment condition of 60-90s.
Listed above is only best specific embodiment of the invention.It is clear that the invention is not restricted to which above embodiments, can also have
Many deformations.All deformations that those skilled in the art directly can export or associate from present disclosure,
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of technique for preparing xanthan gum comprising following steps:
Step 1) will produce cultivates in glue Xanthomonas campestris seed liquor access fermentation medium, and 30 DEG C of temperature, fermented incubation time 60 is small
When, obtain fermentation liquid；
Step 2 is separated xanthan gum feed liquid and wet thallus using high-speed dish piece seperator；
Wet thallus is ultrasonically treated by step 3), and hydrogen peroxide and carbide slag are added into the wet thallus after ultrasonic treatment,
Stirring, is then added in the Dialysis culture base of 3-5 times of weight, cultivates 6-8h, and micro-filtrate membrane filtration collects thallus and filtrate；
Step 4) merges filtrate obtained by the gained xanthan gum feed liquid and step 3) of step 2, for extracting xanthan gum.
2. technique according to claim 1, which is characterized in that during the fermented and cultured, by adjusting speed of agitator
Keeping dissolved oxygen level with ventilatory capacity is 20%, controls residual sugar by auto-feeding glucose solution and is being not less than 1.5%.
3. technique according to claim 1, which is characterized in that the preparation method of the fermentation medium includes following step
It is rapid: to take glucose 9%, soybean protein 0.8%, corn pulp 2.5%, epsom salt 0.3%, phosphoric acid hydrogen two according to weight percent
Potassium 0.3%, potassium dihydrogen phosphate 0.1%, ferrous sulfate heptahydrate 0.001%, VB10.00001%, remaining is water, is stirred evenly,
Adjust pH6.5.
4. technique according to claim 1, which is characterized in that the revolving speed of the high-speed dish piece machine is 4000rpm, and the time is
5min。
5. technique according to claim 1, which is characterized in that the parameter of the ultrasonic treatment: frequency power 400-
600w, ultrasonic action time are each 3s, and interval time 4s, the total time of ultrasonic treatment is 60-90s.
6. technique according to claim 1, which is characterized in that in the step 3), to the wet bacterium after ultrasonic treatment
1wt% hydrogen peroxide and 3wt% carbide slag are added in body, 100rpm stirs 30-60min, is then added to the dialysis training of 3-5 times of weight
It supports in base, 30 DEG C of cultivation temperature, 100rpm stir culture 6-8h, micro-filtrate membrane filtration collects thallus and filtrate.
7. technique according to claim 1, which is characterized in that the Dialysis culture base are as follows: potassium dihydrogen phosphate 0.5%, phosphoric acid
Hydrogen dipotassium 0.5%, ferrous sulfate heptahydrate 0.02%, epsom salt 0.02%, adjustment pH are 6.8, and the above are mass percents.
8. according to claim 1-7 be allowed to one described in technique preparation xanthan gum.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87106960A (en) * | 1987-10-21 | 1988-04-27 | 中国农业科学院土壤肥料研究所 | The production method of low cost xanthocyte gum |
| WO2012030651A1 (en) * | 2010-08-31 | 2012-03-08 | Cp Kelco U.S., Inc. | Xanthan gum with fast hydration and high viscosity |
| CN104805050A (en) * | 2015-02-23 | 2015-07-29 | 泉州市奈斯材料科技有限公司 | Method for accelerating microbial fermentation by negative ion powder |
| CN105463050A (en) * | 2015-12-18 | 2016-04-06 | 内蒙古阜丰生物科技有限公司 | Natural food additive xanthan gum and preparing process thereof |
-
2017
- 2017-11-09 CN CN201711097912.5A patent/CN109762857A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87106960A (en) * | 1987-10-21 | 1988-04-27 | 中国农业科学院土壤肥料研究所 | The production method of low cost xanthocyte gum |
| WO2012030651A1 (en) * | 2010-08-31 | 2012-03-08 | Cp Kelco U.S., Inc. | Xanthan gum with fast hydration and high viscosity |
| CN104805050A (en) * | 2015-02-23 | 2015-07-29 | 泉州市奈斯材料科技有限公司 | Method for accelerating microbial fermentation by negative ion powder |
| CN105463050A (en) * | 2015-12-18 | 2016-04-06 | 内蒙古阜丰生物科技有限公司 | Natural food additive xanthan gum and preparing process thereof |
Non-Patent Citations (4)
| Title |
|---|
| RONG CHENG,ET AL.: "Hydrogen peroxide (H2O2) supply significantly improves xanthan gum production mediated by Xanthomonas campestris in vitro", 《JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY》 * |
| 冯文钦等: "《神奇的矿晶宝石》", 30 September 2015, 地质出版社 * |
| 周建飞: "无细胞体系黄原胶合成机理的初步探索", 《中国优秀硕士学位论文全文数据库•工程科技Ⅰ辑》 * |
| 战佳宇等: "《固体废物协同处置与综合利用》", 31 December 2014, 中国建材工业出版社 * |
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Application publication date: 20190517 |