CN109738627B - A kind of immune method for treating material and its digitization detection tumour Dynamic Mechanism of Chinese medicine - Google Patents
A kind of immune method for treating material and its digitization detection tumour Dynamic Mechanism of Chinese medicine Download PDFInfo
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- CN109738627B CN109738627B CN201811550340.6A CN201811550340A CN109738627B CN 109738627 B CN109738627 B CN 109738627B CN 201811550340 A CN201811550340 A CN 201811550340A CN 109738627 B CN109738627 B CN 109738627B
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Abstract
The present invention relates to Chinese herbal medicine Material Fields, more particularly to the immune method treated material and its detect tumour Dynamic Mechanism of kind Chinese medicine, and the method that related Chinese herbal medicine extracts pick detection, a kind of specific Chinese medicine is immune to treat material, its composed structure are as follows: including centerbody: nano magnetic particle, the nano magnetic particle is equipped with gap, and the centerbody whole installation includes the substance of structural identification digitization measuring mechanism function；The gap of the method Selection Center body magnetic-particle of offer detection tumour Dynamic Mechanism of the invention accounts for the immune treatment material of magnetic-particle certain volume Chinese medicine, after the immune treatment material of Chinese medicine of the above-mentioned selection in the test group injection present invention, it includes current potential, pH value, temperature, nitric oxide parameter that sensor, which is arranged, to test it at test group tumor stomach；A kind of method of fine accurate fast onset immune detection preventive effect ingredient of extraction fructus lycii Radix Astragali Chinese herbal medicine is also provided, and a kind of method that pure natural hippophae brass and sea-buckthorn VC are chosen in calibrating detection is provided.
Description
Technical field
The present invention relates to Chinese herbal medicine Material Fields, more particularly to the immune treatment material of kind Chinese medicine and its digitization detection tumour to move
The method of state mechanism, and the method that related Chinese herbal medicine extracts pick detection.
Background technique
Tumour threatens always the health of the mankind, especially malignant tumour, not only brings great pain to sufferers themselves
Hardship, while also having taken heavy burden to household.Report that (IARC) data are shown according to international cancer research institution, the whole world is altogether
New cancer cases nearly ten million, death has reached million, and existing illness example nearly ten million, developing country's new cases account for
62%, death accounts for 68%, existing illness example and accounts for 58%, and the new cases of cancer in China about 4,000,000, death is about
Have 3,000,000, analyzes 20.12 and 20.18 global pathogenesis of cancer and dead data, trend obviously rise, new cases
78.3% is increased, death increases 69.9%.China is in the whole world and developing country's new cancer cases and death disease
Example accounts for very big ratio.With the rising of Cancer Mortality, efficiently treatment is to get over the attention by everybody.
Doctor trained in Western medicine in terms of mechanism malignant tumour often using operation, chemicotherapy as dominant mechanism means, but in mechanism tumour
While also produce some complication, such as often there are the complication such as pain, Pleural effusions in advanced tumor patient, takes to patient
Pain it is often more serious than tumour itself, radiation mechanism is a kind of local mechanism method using radioactive ray mechanism tumour, mesh
Before have become clinically one of the most frequently used, most effective malignant tumour mechanism means.But radiate mechanism there are still dose of radiations it is high,
It is big to health tissues side effect, the disadvantages of especially tumour cell radioresistant is strong dosage too small not only cannot effectively kill absolutely
Most of cancer cell will increase the radioresistance of residual cancer cells instead, or even induce the mad proliferation and transfer of cancer cell,
Excite the rapid deterioration of patient, at the same doctor trained in Western medicine and its radiochemotherapy to generation act in and its development mechanism of quality cannot slap
It holds and controls, reaction and the deterioration for resulting in treatment are sudden, lack systematic detection Dynamic Mechanism at this stage
Method and shortage obtain the material and process of the lower system dynamical correlation parameter of immune system running, do not formed dynamically
The method that mechanism and its burst procedures burst reason detect under system, does not join the stage mechanism of malignant development and its data
Number dynamically tests and analyzes providing method under system and prejudges with reference to mechanism, is detached from immune system and participates in, and shortage tumour is dynamically
Mechanism Detection of Process Parameters system method is drilled without process and is prejudged, and also just causes the deterioration in effect especially western medical treatment prominent
Hair property, one of side effect disadvantage method process factors outstanding.
Chinese medicine effective stabilization in terms of mechanism tumour promotes malignant development and its sudden especially for malignant tumour
It is smaller with the side effect to entirety, but Chinese medicine is good at long term, quickly controls to tumour and pinpoints eradicating efficacy not
Obviously, while the preferable therapeutic effect of Chinese medicine mainly carries out discriminatory analysis according to effect at this stage, and shortage moves advantage curative effect
The method that state mechanism is tested and analyzed lacks the method for the carry out analysis detection by its therapeutic mechanism supplemental characteristic of system
And material, lack and test and analyze mechanism dynamic parameter to contain that tumour Power evelopment provides support with reference to anticipation, it is swollen to lack grasp
The dynamic of tumor crosses feature journey physical and chemical parameter digitization grasp, and Chinese medicine is also just caused further not refine development by high-efficiency precision,
So that advantages of TCM is not had considerable performance, traditional Chinese medicine and other therapies do not obtained cooperateing with optimal combination yet, it is existing in
Herbal medicine has effect on improving immunity and its prevention of lesion detection analysis mechanism, but in existing Chinese herbal medicine use process
It is more extensive, there is the substance preferably acted at using less, the substance of the faint or even side effect of effect or impact effect is more or deposits
And relevant effect accurate to the detection method of tumour is being influenced, existing technology shortage religion is quasi- accurately quickly to be obtained having accurately
The method and process of the medicinal herb components of accurate impression, at the same also lack quick pick detection good action medicinal ingredient method and
Technique.
Summary of the invention
A series of concept of reduced forms is introduced in Summary, this will in the detailed description section into
One step is described in detail.Summary of the invention is not meant to attempt to limit technical solution claimed
Key feature and essential features do not mean that the protection scope for attempting to determine technical solution claimed more, hereafter
Present invention is described for illustrating technical solution method and step.
In order to which solving the above problems for system of systems treats material and its digitization inspection the present invention provides a kind of Chinese medicine is immune
The method for surveying tumour Dynamic Mechanism, a kind of Chinese medicine is immune to treat material, and composed structure are as follows: including centerbody: it includes nano magnetics
Property particle, the nano magnetic particle be equipped with gap, the centerbody whole installation include structural identification digitization test
The substance of mechanism function.
Further, further include around body: further including around body: the surrounding body and centerbody solid arrangement and two
The corresponding substance equipped with structural identification digitization measuring mechanism function on person's ontology, it includes poly- Paederia scandens that surrounding body, which is constituted,
Chinese medicine immune substance is combined on glycosides methacrylate polymer nano molecular chain, the partial size of the surrounding body is described not less than 25 nanometers
The partial size of centerbody is not more than 10 nanometers, is coordinated jointly by the corresponding substance of the size structure of centerbody and surrounding body
Specific position plays a role, and the quantity of the surrounding body and the quantity ratio of the centerbody are (80-150): 1, the material hair
Body is present in around centerbody and is distributed or is present in 3 D stereo space surface around it when waving effect, the corresponding center
Body exists among the 3 D stereo constituted with the center of body around or surrounding body, and the polymer nanocomposite strand further includes using second
Acyl woodruff acid glycosides methyl esters, glycine betaine polymerization, the molecular weight 10 of the polymer nanocomposite molecule4-106, Chinese medicine immune substance includes
Polysaccharides, asperuloside, sea-buckthorn brass, Tanshinone II, danshensu, sea-buckthorn VC, the nano magnetic particle surface is equipped with
Gap, the nano magnetic particle surface are provided with bismuth manganese Ce-Al alloy or its oxide while the gap being made to constitute coating sky
Gap, the gap of the coating account for the 60%-80% of magnetic particle size, at least provided with basic oxide in the coating gap
Salt, to provide the material of the detection tumour mechanism of dynamic system digitization.
Further, the magnetic-particle is nano oxidized iron particle, and the basic oxide salt is percarbonic acid ammonium or mistake
Sodium carbonate.
In order to solve the above problems that invention also provides the materials described in one kind with above-mentioned material system of systems
Manufacturing method comprising:
Have in the pressure-bearing filter cylinder of 5-7 nanofiltration membrane step 1: choosing 6-8 nano magnetic particle and filling Single port, it is described to hold
The preferred 500ml of filters pressing cylinder, is then passed through pressure 0.3-0.6Mpa from the other end of filter cylinder, and the hydrochloric acid mist 8 of temperature 30-40 degree arrives
20 hours, containing percentage by volume it is 15-25% nitrogen and vapor in the hydrochloric acid mist, is then put into magnetic-particle super
After sonication is cleaned and dried, one layer 0.5-1 nanometers bismuth manganese Ce-Al alloy or its oxidation are then connect in its electroplating surface or painting
Magnetic-particle after coating is finally soaked into after the SODIUM PERCARBONATE of full high concentration first and impregnates high concentration percarbonic acid again by object coating
Ammonium salt solution makes gap fill centerbody described in the anhydrous formation of flat rear low-temperature air-drying；
Step 2: with 10-20g Paederia scandens time glycosides methyl esters and including using acetyl woodruff acid glycosides methyl esters 5-8g or glycine betaine 3-
5g incorporates in 70-100g water, and 22-35 DEG C of initiation polymerization, regular oxidation initiator causes, and amount of initiator is aqueous solution weight percent
The 0.2-0.6% of ratio, molecular weight are controlled 103-104Afterwards, Qi polysaccharide, asperuloside, danshensu is added and sea-buckthorn VC is added simultaneously,
Sea-buckthorn VC amount is amount of initiator 5/10ths to ten/10th, to adjust the molecular structure and protect immune object that reaction generates
Matter, being warming up to 38-42 degree to react 10-30 minute postscripts is A liquid, then will be in other including sea-buckthorn brass, Tanshinone II
Medicine immune substance is added including in the organic solvent including ethyl acetate, the amount of the organic solvent is the 1.1-1.4 of water
Times, 35-40 is heated with stirring to after the sorbester p17 and Tween20 of the total 0.2-0.6g of weight is then added in the organic solvent
Degree postscript is B liquid, and A liquid addition B liquid 15-30 minutes removing organic solvents of mechanical oscillation emulsion reaction under 35-40 degree are formed
Surrounding body, the addition quality including Qi polysaccharide, asperuloside, danshensu, sea-buckthorn brass, Tanshinone II is Immune discrimination knot
Structure instrumentality includes Paederia scandens time glycosides methyl esters and includes the 80- for using acetyl woodruff acid glycosides methyl esters or betaine polymer quality
120 times, the ratio of the weight of the sorbester p17 and Tween20 is 15-5 than 1, so that the corresponding molecular weight of surrounding body generation and space
Structure nano substance；
Step 3: by the first step centerbody with the 1:72--150 by mass percentage of the middle surrounding body in second step
Ratio form the immune one kind for treating material of the Chinese medicine after mixing and be sealed.
The above material is that the dynamic detection of the mechanism parameter of immune system and effect has step to have being combined into for timing
Indispensable entity plays a role.
The material is also applied in the method for digitization detection tumour Dynamic Mechanism by the present invention simultaneously, specifically,
The first step selects suitable experiment mice to be randomly divided into several groups, straight to the mixing food of its feeding nitrite and coal tar
Neoplastic lesion is generated to its stomach, observation test passes through and sensor is arranged at its tumor stomach when tumour growth to diameter 3cm
Testing it includes current potential, pH value, temperature, nitric oxide parameter；
Step 2: wherein the first group and second group radiation for awarding multiple micro- dosage, test are observed its tumor size and are become for choosing
It changes until generating the malignant change of diameter 1-2cm or starting to shift.
Step 3: the gap of Selection Center body magnetic-particle accounts for, magnetic-particle certain volume Chinese medicine is immune to treat material, to the
After the immune treatment material of Chinese medicine of above-mentioned selection in one group of injection present invention, while being lower than by the heating of electromagnetic wave irradiation centerbody
40 degree make percarbonate in its gap all decompose and go out by decomposing the gas generated, pass through center described in Imaging Study
After body is distributed in tumour cell center, and surrounding body is distributed in the effect a period of time of the surrounding centered on tumour cell,
It includes current potential, pH value, temperature, nitric oxide parameter that sensor is arranged at its tumor stomach to test it, is judged by sound wave image
When in centerbody gap percarbonic acid object decompose complete when, and at its tumor stomach be arranged sensor test it include current potential,
PH value, temperature, nitric oxide parameter, and pass through the size and its tumour dynamic machine of parameter and Imaging Study judgement record tumour
It manages, while determining the amount and tumour of corresponding basic oxide salt or oxide or percarbonate according to above-mentioned tumour dynamic change
Identification object sensitivity is to instruct detection or Analysis on Mechanism, while as a control group with second group, corresponding in its tumor stomach
It includes current potential, pH value, temperature, nitric oxide parameter that place's setting sensor, which tests it, and records corresponding sound wave image parameters, will
The stage situation of parameter information record system chemical conversion data and corresponding reaction kinetic tumor development in the above process, for precisely inspection
Observing and controlling dynamic tumour providing method.
Above-mentioned material and its material of detection mechanism method having the beneficial effect that through the invention and see detection method entirety
Providing method is dynamically tested and analyzed under system to the stage mechanism and its data parameters of malignant development under coupled system effect
It is prejudged with reference to mechanism, medicinal material and its method promote that immune system is combined to participate in, and method system is embodied in tumour dynamic machine
Reason phase process parameter detecting and digitization are shown, realize the rehearsal anticipation of integral system process, especially for lesion detection control
The sudden reason system Dynamic Mechanism of deterioration in western medical treatment, side effect disadvantage outstanding provide detection method and digitization
For parameter as reference, the Dynamic Mechanism process feature physical and chemical parameter data for also grasping tumour turn to the further efficient fine of Chinese medicine
Development offer Physico-chemical tests method is provided and foundation is cooperateed with traditional Chinese medicine with other therapies to play advantages of TCM
Optimal combination provides method material.In addition the method for above-mentioned material detection mechanism is organically matched in whole real-time collaborative system
Close lower realize.
In order to solve the problems, such as one of nonsystematic system in above-mentioned background, the present invention provides a kind of fructus lyciis or astragalus polyose to mention
Taking technique method；Step 1: using new fresh fructus lycii or Radix Astragali as raw material, by mass fraction 60-79% ethyl alcohol and matter after being crushed
Amount score 1-5% citric acid solution 60:1 adds mass fraction 10-20% positive charge resin after being added and impregnates under 30-50 degree
2-8h blasts methane microbubble 10-30min；
Step 2: static 5-20min is cooled to 4-12 degree simultaneously, and filtrate then is collected by filtration with mocromembrane, is warming up to 30-50
Degree adds 5-10g sodium bicarbonate nanofiltration membrane.Step 3: and then simultaneously with the hot water backwash filter membrane under 0.2-0.4Mpa
Rinse solution is recycled, mass percent 0.05-0.5% vinyl alcohol, mass percent is added in the solution after recycling is rinsed
0.1-0.3% acetic acid and few drops of potassium permanganate, while center is added and is enclosed with nano aperture magnetic powder alkalescent azanol styrene resin
It is first to heat to after 30-45 degree after being kept for 20-50 minutes and freezes again to 3-8 degree after rouge, then attracted with magnetic sucking tube and separate the resin,
Then will inhale have resin magnetic sucking tube be put into normal saline solution by switch magnetic and adjust magnetic force size accurately quickly divide
From the small molecular polysaccharide in the resin, solvent flashing obtains polyose needed for paste.
Process for extracting of the invention also allows it finely accurately quickly to send out with accurately selection Chinese herbal medicine and its ingredient
It waves it and is improving the effect in immunity and its prevention of lesion detection analysis mechanism, this method has to be obtained under limited working condition
The small molecular polysaccharide with immune function is quick and precisely obtained, working condition facility environment is simple and direct.
One of above-mentioned in order to solve the problems, such as, the present invention also provides a kind of method that high-purity sea-buckthorn brass VC detection is chosen；
Step 1: therapeutic medical Hippophate flavone and sea-buckthorn VC to be made to thin crystal light transmitting sheet, pass through its light transmittance of ultraviolet optical tests, choosing
Numerical value percentage of the light transmittance compared with the light transmittance of corresponding standard Hippophate flavone or corresponding standard sea-buckthorn VC is selected to be not less than
Substance corresponding to 96%, step 2: then obtaining its Electronic Speculum by choosing the structure of substance in the above-mentioned first step of electron-microscope scanning
It is similar to standard picture to choose subject image by it in standard Hippophate flavone or sea-buckthorn VC sem image comparative analysis for image
Degree is not less than the corresponding substance of 99.5% image, while needing the 99% nonbody image and mark of the total image area of subject image Zhan
The similarity of quasi- image is not above substance corresponding to 30% image.Step 3: by the sea-buckthorn chosen in above-mentioned second step Huang
Copper and sea-buckthorn VC carry out the infrared spectrum for going out corresponding infrared spectrum, choosing in sea-buckthorn brass and sea-buckthorn VC of examination of infrared spectrum
In secondary peak peak type connecting structure at least three is consistent or spectrogram secondary peak peak type and the corresponding substance of the similar spectrogram of connecting structure
For the substance being selected.
It further include that the peak on the spectrogram is corresponding with the ordinate value of peak type curve in the further third step
Length and peak type curve be open closure after abscissa distance calculate peak type constitute area process, by peak type area value
It is the peak of the 0.5-5% of maximum peak type area figures is determined as secondary peak, the peak type and connecting structure are similar including peak area
Size is in 1% and the numerical value and graphic feature at the feature of peak curvilinear figure and close peak connected to it are close, described identical
Under the conditions of carried out in parallel as the sea-buckthorn be similar or sea-buckthorn that ambient growth conditions are similar, the environmental condition tested,
Parameter is identical, and described peak type connecting structure at least three unanimously includes following characteristics absorption peak 3523cm- 1, 3412cm- 1,
3378cm- 1,2927cm- 1,1564cm- 1,1695cm- 1,1631cm- 1,821cm- 1Occur, the construction of above-mentioned peak type includes absorbing
At least one peak valley is connected between peak every two.
Further, the above method can choose work using the quick detection with flavones and polysaccharide in Radix Salviae Miltiorrhizae and Radix Notoginseng
Skill, it is characterised in that: including sample preparation is surveyed in faintly acid low temperature environment by therapeutic medical natural Radix Salviae Miltiorrhizae or Radix Notoginseng flavones and polysaccharide
Try is rapid.
Further, the preferred pH of the weak acid environment be 5-6, by under the conditions of temperature 3-5 degree by 1-3g ice vinegar
Acid is mixed with 20-30g potassium bromide to be produced the sampling to be checked of Radix Notoginseng or Radix Salviae Miltiorrhizae and carries out detection selection.
The detection of above-mentioned pick choose pure Chinese herbal medicine method pass through more than choosing method to fast implement selection inspection not natural
Pure Hippophate flavone and sea-buckthorn VC, and detected and chosen come effectively simple and direct realization by autologous correlation compatibility, prevent simultaneously
The only control of non-natural artificial synthesized VC, polysaccharide and flavonoids object, while reducing the cumbersome step process of traditional chemical pick,
With the operating instruction and experiment condition of stringent duration, have the characteristics that quick pick detects good pure effect medicinal ingredient effect.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon: the following drawings of embodiment of the present invention is in this as a part of the invention for managing
The solution present invention.Shown in the drawings of embodiments of the present invention and its description, principle used to explain the present invention.In the accompanying drawings,
Fig. 1: the infrared light map of natural Chinese wolfberry fruit or Radix Astragali standard polysaccharide；
Fig. 2: natural standard Hippophate flavone or natural standard sea-buckthorn VC electron-microscope scanning image.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
Attached drawing, the technical solution of the embodiment of the present invention is clearly and completely described.Obviously, described embodiment is this hair
Bright a part of the embodiment, instead of all the embodiments.Based on described the embodiment of the present invention, ordinary skill
Personnel's every other embodiment obtained, shall fall within the protection scope of the present invention.
A kind of Chinese medicine of the invention is immune to treat material, and composed structure includes the following: centerbody: including nano-magnetic
Grain, the magnetic-particle are nano oxidized iron particle, and the ferric oxide nano magnetic-particle is equipped with gap, and the centerbody is whole
Substance of the body setting comprising structural identification digitization measuring mechanism function；Further include around body, the surrounding body and it is described in
The corresponding substance equipped with structural identification digitization measuring mechanism function in heart body solid arrangement and the two ontology；Solid arrangement
And the corresponding substance concrete condition equipped with structural identification digitization measuring mechanism function on the two ontology are as follows: surrounding body is constituted
Including being combined with Chinese medicine immune substance on poly- Paederia scandens time glycosides methacrylate polymer nano molecular chain, the partial size of the surrounding body is not small
In 25 nanometers, the partial size of the centerbody is not more than 10 nanometers, the quantity and the quantity number of the centerbody of the surrounding body
Than for (80-150): 1, item is provided by the corresponding measuring mechanism that is set as of the entirety of the partial size and quantity of body around and centerbody
Part, body is present in around centerbody and is distributed or is present in 3 D stereo space surface around it when the materials serve acts on,
The corresponding centerbody, which exists, has constituted centerbody and week among the 3 D stereo constituted with the center of body around or surrounding body
The containment body structure composition that Coordinated Play acts under system space, the polymer nanocomposite strand further include with acetyl woodruff
Sour glycosides methyl esters, glycine betaine polymerization, the molecular weight 10 of the polymer nanocomposite molecule4-106, Chinese medicine immune substance includes that fructus lycii is more
Sugar, asperuloside, sea-buckthorn brass, Tanshinone II, danshensu, sea-buckthorn VC, the sea-buckthorn VC are used to protect and coordinate described
Chinese medicine immune substance synthesis plays a role, and the nano magnetic particle surface is equipped with gap, the nano magnetic particle surface
It is provided with bismuth manganese Ce-Al alloy or its oxide while the gap being made to constitute coating gap, the gap of the coating accounts for magnetic
The 60%-80% of plastochondria product, the basic oxide at least provided with basic oxide salt in the coating gap, in the gap
Salt is able to cooperate under magnetic particle conditions control and plays a role, such as real in the case where magnetic material structures design is by long-range light-operated heat
It now decomposes and generates alkaline gas, oxidizing substance, the alkaline gas and oxidizing substance are to identify to provide ring with antitumor
Border and substance, and generate quantization void space and detect the acousto-optic iconography point tracer that mechanism judges stage instruction,
The nano magnetic particle material the Nomenclature Composition and Structure of Complexes provides heating, position, reaction condition control and data for Composition analyzed
The condition for changing detection is controlled by the above material, structure, positional relationship, condition, the cooperation of parameter setting combined system and in real time association
With the lower detection tumour mechanism for completing dynamic system digitization, the preferably described basic oxide salt is percarbonic acid ammonium or percarbonic acid
Sodium, to provide the material of the detection tumour mechanism of dynamic system digitization.
Illustrate the manufacturing method and action principle of material of the present invention below, specifically technique includes the following steps:
Embodiment one；
There is the pressure-bearing of 5 nanofiltration membranes to filter step 1: the preferred ferric oxide particles of magnetic-particle for choosing 6 nanometers fill Single port
In cylinder, it then is passed through pressure 0.3Mpa from the other end of filter cylinder, the hydrochloric acid mist that 30 degree of temperature 8 hours, in the hydrochloric acid mist
It is 15% nitrogen and vapor containing percentage by volume, above-mentioned acid mist work carries out in the ventilating kitchen of closed recyclable recycling,
Gap has been formed, after magnetic-particle is then put into cleaned by ultrasonic vibration drying, magnetic-particle is then put into ultrasonic wave vibration
After swinging cleaning-drying, gap coating then is formed in the bismuth manganese Ce-Al alloy coating that its electroplating surface or painting connect one layer 0.5 nanometer
Or coating gap,
Finally the preferred ferric oxide particles of magnetic-particle after coating are soaked into after the SODIUM PERCARBONATE of full high concentration again first
Impregnating high concentration percarbonic acid ammonium salt solution makes gap fill centerbody described in the anhydrous formation of flat rear low-temperature air-drying；
Step 2: with 10g Paederia scandens time glycosides methyl esters and including being incorporated with acetyl woodruff acid glycosides methyl esters 5g or glycine betaine 3g
In 70g water, 22 DEG C of initiation polymerizations, the initiation of regular oxidation initiator, such as ammonium persulfate, amount of initiator is aqueous solution weight percent
0.2%, molecular weight control 103Afterwards, Qi polysaccharide, asperuloside, danshensu is added and sea-buckthorn VC is added simultaneously, sea-buckthorn VC amount is
Amount of initiator 1/10th is warming up to 38 degree and reacts 10 points to adjust the molecular structure and protection immune substance that reaction generates
Clock postscript is A liquid, then it includes using ethyl acetate that other Chinese medicine immune substances including sea-buckthorn brass, Tanshinone II, which are added,
In organic solvent inside, the amount of the organic solvent is 1.1-1.4 times of water, and matter then is added in the organic solvent
35 degree of postscripts are heated with stirring to as B liquid after measuring the ratio sorbester p17 and Tween20 of 0.2g, and the mechanical vibration under 35 degree of B liquid is added in A liquid
It swings 15 minutes removing organic solvents of emulsion reaction and forms surrounding body, described includes Qi polysaccharide, asperuloside, danshensu, sea-buckthorn Huang
Copper, Tanshinone II addition quality be Immune discrimination structure instrumentality include Paederia scandens time glycosides methyl esters and include with acetyl woodruff
The ratio of 80 times of sour glycosides methyl esters or betaine polymer quality, the sorbester p17 and Tween20 are 15 to 1, so that surrounding body
Generate corresponding molecular weight and space structure nano-substance；
Step 3: by the centerbody in the first step and the ratio with the 1:72 by mass percentage of the middle surrounding body in second step
The immune one kind for treating material of the Chinese medicine is formed after mixing to be sealed, in addition can also with centerbody or the mixing of surrounding body or
The two is constituted with the mixing of other ratios, and various Chinese medicines are immune to treat material.
Embodiment two: step 1: choosing 8 nanometers of ferric oxide particles fills the pressure-bearing filter cylinder that Single port has 7 nanofiltration membranes
In, it then is passed through pressure 0.6Mpa from the other end of filter cylinder, the hydrochloric acid mist that 40 degree of temperature 20 hours contains in the hydrochloric acid mist
Having percentage by volume is 25% nitrogen and vapor, and above-mentioned acid mist work carries out in the ventilating kitchen of closed recyclable recycling,
Gap is formed, after magnetic-particle is then put into cleaned by ultrasonic vibration drying, then one layer 1 is connect in its electroplating surface or painting and receives
The bismuth manganese Ce-Al alloy oxide coating of rice forms gap coating or coating gap, finally by the preferred oxygen of magnetic-particle after coating
Change iron particle be soaked into after the SODIUM PERCARBONATE of full high concentration first impregnate again high concentration percarbonic acid ammonium salt solution make gap fill it is flat after
Centerbody described in the anhydrous formation of low-temperature air-drying；
Step 2: with 20g Paederia scandens time glycosides methyl esters and including being incorporated with acetyl woodruff acid glycosides methyl esters 8g or glycine betaine 5g
35 DEG C of initiation polymerizations in 100g water, the initiation of regular oxidation initiator, such as potassium peroxydisulfate, amount of initiator are aqueous solution weight percent
0.6%, molecular weight control 104Afterwards, Qi polysaccharide, asperuloside, danshensu is added and sea-buckthorn VC is added simultaneously, sea-buckthorn VC amount is
Amount of initiator, to adjust the molecular structure and protection immune substance that reaction generates, is warming up to 42 degree of reactions 30 to 5/10ths
Minute postscript is A liquid, then it includes using acetic acid second that other Chinese medicine immune substances including sea-buckthorn brass, Tanshinone II, which are added,
In organic solvent including ester, the amount of the organic solvent is 1.1-1.4 times of water, is then added in the organic solvent
Being heated with stirring to 40 degree of postscripts after the sorbester p17 and Tween20 of the total 0.2-0.6g of weight is B liquid, and A liquid is added under 40 degree of B liquid
30 minutes removing organic solvents of mechanical oscillation emulsion reaction form surrounding body, it is described include Qi polysaccharide, asperuloside, danshensu,
Sea-buckthorn brass, Tanshinone II addition quality be Immune discrimination structure instrumentality include Paederia scandens time glycosides methyl esters and include using acetyl
The ratio of 120 times of woodruff acid glycosides methyl esters or betaine polymer quality, the sorbester p17 and Tween20 are 5 to 1, so that
Surrounding body generates corresponding molecular weight and space structure nano-substance；
Step 3: by the centerbody in the first step and the ratio with the 1:150 by mass percentage of the middle surrounding body in second step
Example forms the immune one kind for treating material of the Chinese medicine after mixing and is sealed.
The method and action principle of the material dataization detection tumour Dynamic Mechanism of the invention is illustrated below, including
Step: step 1: selecting suitable experiment mice to be randomly divided into several groups such as F344 (CDF) mouse is divided at least two groups, every group 5
Only, for the mixing food to its feeding nitrite and coal tar until its stomach generates neoplastic lesion, detailed process is to give daily
The mixing food 1:1 of 2 feeding sodium nitrites of mouse and coal tar amounts to 1.5g continuous two months to tumour is generated, and observation test is worked as
Tumour growth is to diameter 3cm, and by selecting IMFI type (current potential/temperature/pH) comprehensive tester sensor and NO-A4 is high score
It includes current potential, pH value, temperature, nitric oxide parameter etc. at its tumor stomach that the nitric oxide sensor probe of resolution, which detect,
The average value of parameter, the above-mentioned parameter of two group of ten mouse is respectively current potential: 135MV, pH:5.8, temperature: 38.5 degree, an oxidation
Nitrogen: 58 μm of ol/L；
Step 2: the choosing wherein first group and second group radiation for awarding multiple micro- dosage, preferred dose is that 36 gray(Gy)s are each,
Continuous one week, test observed the transformation of its tumor size until generating the malignization lesion of diameter 2cm or starting to shift, with IMFI type
(current potential/temperature/pH) comprehensive tester sensor and NO-A4 are that high-resolution nitric oxide sensor probe carries out detecting it
It include the parameters such as current potential, pH value, temperature, nitric oxide parameter, the average value of the above-mentioned parameter of two group of ten mouse at tumor stomach
Respectively, current potential: 138MV, pH:5.9, temperature: 39 degree, nitric oxide: 50 μm of ol/L；
Step 3: treating material, the specific course for the treatment of to the Chinese medicine in first group of injection present invention in above-mentioned second step is immune
It is continuous 3 weeks 3 times a day, the gap of Selection Center body magnetic-particle accounts for the immune treatment material of 60% Chinese medicine of magnetic particle size,
The dose of material is 60-110mg/m2, and it is thin to be then distributed in tumour by centerbody described in CT or nuclear-magnetism sound wave Imaging Study
In born of the same parents center, and after surrounding body is distributed in the effect a period of time of the surrounding centered on tumour cell, the preferably two Monday courses for the treatment of
Afterwards, sensor testing parameter data is set at its tumor stomach comprising current potential 139MV, pH:5.9, temperature: 38 degree, an oxygen
Change nitrogen: 49 μm of ol/L, is contrasted by test forming process detection and with subsequent dynamic process, is conducive to immune body
The grasp of the dynamic analysis and change direction of the process of system's effect and physical and chemical effect mechanism, at the same by the magnetron of electromagnetic wave with
The frequency of 1500-2600MHZ, which launches the heating of microwave irradiation centerbody, decomposes percarbonate in its gap all
And by decompose generate gas go out, wherein the gas and substance of the generation of ammonium salt in whole installation of the present invention for identification and
Detection index substance, is judged by sound wave image when the percarbonic acid object in centerbody gap decomposes completion, in its tumor stomach
It is a high-resolution oxygen that it includes with IMFI type (current potential/temperature/pH) comprehensive tester and NO-A4 that place's setting sensor, which tests it,
Change the average result that the test of nitrogen sensor obtains following first group of 5 each parameter, current potential: 130MV, pH:6.2, temperature: 38 degree,
Nitric oxide: 45 μm of ol/L reduce 1-0.8cm by acoustic imaging and CT and magnetic resonance imaging comprehensive descision diameter of tumor, in the middle
When percarbonic acid object in heart body gap decomposes completion, since the structural material setting of centerbody is determined when non-in centerbody gap
Percarbonate or the significantly different vascular tissue's situation that may determine that around tumour of its sound wave image parameter and fixed when thering is water to enter
Position tumour intrusion tissue is depth and direction, and it includes current potential, pH value, temperature that sensor, which is arranged, to test it at its tumor stomach
Degree, nitric oxide parameter, and pass through the size and its mobile knot of tumor image variation of parameter and Imaging Study judgement record tumour
Close corresponding current potential, ph, nitric oxide, temperature, correlation when becoming smaller that the Parameters variations such as current potential, pH, nitric oxide judge tumour
The judge of the Dynamic Mechanism of the systems such as tissue and depth of invasion of connection variation and the result that accurately quantizes is quantitative, while according to upper
State amount and tumour the identification object sensitivity journey that tumour dynamic change determines corresponding basic oxide salt or oxide or percarbonate
Degree is to instruct detection or Analysis on Mechanism, such as by above-mentioned can determine Chinese medicine and alkali formula peroxide as cooperative drug system while saying
Recognition reaction and ammonium salt product and Paederia scandens time glycosides methyl esters and acetyl woodruff acid glycosides that bright ammonium material and Chinese medicine have had
Methyl esters or glycine betaine etc. identify that object to the identity and sensibility of tumour, such as judges above-mentioned object by acousto-optic image and parametric synthesis
Matter tumor vicinity assemble and act on after Parameters variation it is big, illustrate that identity and sensitivity are preferable, it is subsequent can be comprehensive according to parameter etc.
It closes variation and further instructs mechanism detection and analysis, peroxidating can be calculated additionally by the gap of centerbody and the variable quantity of tumour
Object or oxide act on how many relationship, and other oxidation numerical value are calculated such as the alkali formula percarbonate in the present embodiment, specifically may be used
It is determined by injection volume harmony optical image magnetic by centerbody after positioning participates in the immune treatment material and centerbody that play a role
The numerical value meter (such as gap account for particle volume 60%) that the gap of particle accounts for magnetic particle size calculates the amount of percarbonate, into one
Step show that the parameter of oxidation material or oxidation state and the corresponding state for obtaining tumour under the oxidation too parameter such as size, current potential become
Change etc., while as a control group with second group in above-mentioned second step, the immune treatment material of Chinese medicine in the present invention is not injected, it is corresponding
Second group of 5 middle tumor stomach at setting sensor test the average value of its each parameter, including parameter is current potential: 147MV, pH
Value 5.2, temperature: 39.6, nitric oxide: 58 μm of ol/L, and record corresponding sound wave, CT images image parameters show tumour become larger
1.5cm, tumour invade tissue and go deep into, developing direction surrounding, and the information such as liquid viscosity is larger are entered in centerbody gap.
The method beneficial effect data of specific material and its digitization detection tumour Dynamic Mechanism are as follows from the above
Table 1, the average deviation calculated value of test data is 1-2 in table, with the identity of any two mouse in group mouse under the same terms
Can parameter phase absolute value of the difference be 0.5-1.5, by tumor size in the third line in conditions and data comparison and table, generation
Material and usage thereof of the invention known to nitrogen oxide and current potential ph has tumour dynamic after acting on the tumour of deterioration or transfer
Apparent effect has obtained the parameter of the Dynamic Mechanism in tumour each stage by this detection method, as each tumour is dynamic in table 1
State changes phase parameter corresponds to its Dynamic Mechanism process, and the comprehensive variation such as characteristic parameter such as current potential, nitric oxide represents mechanism
Moment, can for tumour control and mutation analysis presentation mode guide for method, the mode of this detection tumour Dynamic Mechanism
Effect is also embodied in the rank of parameter information record system chemical conversion data and corresponding reaction kinetic tumor development in the above process
The dynamic parameter of corresponding various burst mechanism during tumour became larger and becomes smaller in the 1-2cm stage in the section situation such as present invention
Data in table are such as made into data interval by the parameters relationship between information and medication tumour variable quantity, as tumour current potential exists
By other data in table and the comprehensive confirmation of above process mode is detectable show that tumour is in benign development when 133-130
Situation interval parameter information, the above-mentioned section of result effect after effect and anticipation survival dose can also be estimated by this detection mode
Under parameter information, can further be mentioned by the detection mode by computer data information for precisely detection control dynamic tumor
For method or the dynamic trend parameter information of anticipation reference is provided, the present invention is the Dynamic Mechanism number of clear observing and controlling tumour in a word
Characteristic parameter or the information for the mechanism process that happens suddenly under tumor architecture are grasped according to process providing method is changed to realize detection tumour dynamic
The method of Dynamic Mechanism process under mechanism and its system.
Table 1
In order to obtain being suitable for quick activating immune system under limited working condition and for acting on tumour cell
The polysaccharides of traditional Chinese medicine of detection, and Chinese herbal medicine brass and VC are obtained, illustrate the preparation method of polysaccharide by taking fructus lycii or Radix Astragali as an example below,
Illustrate the method for quickly realizing that its system inspection is integrated by taking sea-buckthorn brass and VC as an example.
The present invention also provides a kind of fructus lycii or astragalus polyose process for extracting, embodiments one: including step 1: with fresh
Fructus lycii or Radix Astragali are raw material, by mass fraction 60-79% ethyl alcohol and mass fraction 1-5% citric acid solution 60:1 after being crushed
Mass fraction 10-20% positive charge resin is added after addition and impregnates 2-8h under 30-50 degree, and the positive charge resin is preferred
Polyurethane or carbonic ester, blasting methane microbubble (assembles macro-molecular protein and rouge egg interface occurs) 10-30min, step
Two: static 5-20min is cooled to 4-12 degree simultaneously, and filtrate then is collected by filtration with 0.1-2 μm of mocromembrane, to realize except grease and
Protein structure composition is adjusted, 30-50 degree is warming up to and adds 5-10g sodium bicarbonate nanofiltration membrane, with regulatory protein matter
It is matched with the structural constituent of polysaccharide, step 3: and then backwash filter membrane with the hot water under 0.2-0.4Mpa and recycle rinse solution,
Recycling rinse after solution in be added mass percent 0.05-0.5% vinyl alcohol, mass percent 0.1-0.3% acetic acid and
Few drops of potassium permanganate allow the aggregation of sized molecules albumen and the easily inspection of plastic bulk precipitating not to separate, while center is added is enclosed with and receiving
Metre hole diameter magnetic powder alkalescent azanol styrene resin, magnetic powder make magnetic force and resin charge effect keep resin adsorption power stronger simultaneously,
Styrene type low pole copolymer, the resin described in nano aperture, which prevents from reacting insufficient albumen and is mixed into, causes separation drop effect,
The nonacid polysaccharide of small molecular, the sugar for surveying tumour mechanism, after be first to heat to after 30-45 degree kept for 20-50 minutes after make again
Be cooled to 3-8 degree, then attracted with magnetic sucking tube and separate the resin, by above can precisely high-purity protein isolate and required polysaccharide,
Equipment is few, good in economic efficiency, then will inhale the magnetic sucking tube for having resin, is provided with current control suction in the magnetic sucking tube
The electromagnet of size, magnetic force size control the weight in production containers half volume magnetic powder, above-mentioned magnetic sucking tube are put into physiology salt
Aqueous solution is by switch magnetic and adjusts magnetic force size or is completed in 10 minutes by small to maximum magnetic force with accurately quick point
From the small molecular polysaccharide in the resin, destroying group performance to prevent polysaccharide molecule influences, and solvent flashing obtains needed for paste
Polysaccharides product.
Embodiment two:: including step 1: using fresh Radix Astragali as raw material, by mass fraction 60-79% ethyl alcohol after being crushed
Mass fraction 10-20% positive charge resin is added under 30-50 degree after being added with mass fraction 1-5% citric acid solution 60:1
2-8h is impregnated, blasting methane microbubble (assembles macro-molecular protein and rouge egg interface occur) 10-30min, step 2: static
5-20min is cooled to 4-12 degree simultaneously, and filtrate (except grease and adjustment protein structure composition) then is collected by filtration with mocromembrane, rises
Temperature to 30-50 degree adds 5-10g sodium bicarbonate nanofiltration membrane (regulatory protein matter and the structural constituent of polysaccharide proportion), step
Rapid three: and then with the hot water backwash filter membrane under 0.2-0.4Mpa and rinse solution is recycled, add in the solution after recycling is rinsed
Enter mass percent 0.05-0.5% vinyl alcohol, mass percent 0.1-0.3% acetic acid and few drops potassium permanganate (allow sized molecules
Albumen aggregation and the easily inspection of plastic bulk precipitating not Fen Li), while center is added and is enclosed with nano aperture magnetic powder alkalescent azanol benzene
(styrene type low pole copolymer, the resin described in nano aperture prevents from reacting insufficient albumen and is mixed into be caused vinyl
Separation drop effect, the nonacid polysaccharide of small molecular, the sugar is for surveying tumour mechanism) after be first to heat to 30-45 degree after keep 20-50 to divide
Zhong Houzai freezes to 3-8 degree, is then attracted with magnetic sucking tube and separates the resin, (by above can precisely high-purity protein isolate and
Required polysaccharide, equipment is few, good in economic efficiency), the magnetic sucking tube for having resin then will be inhaled, is provided with electricity in the magnetic sucking tube
The electromagnet of flow control suction size, magnetic force size controls the weight in production containers half volume magnetic powder, by above-mentioned magnetic sucking tube
Be put into normal saline solution by switch magnetic and adjust magnetic force size or in 10 minutes complete by it is small to maximum magnetic force with
Small molecular polysaccharide (destroying group performance to prevent polysaccharide molecule influences) in resin described in accurate quick separating, solvent flashing obtains
To astragalus polyose product needed for paste.
By fructus lycii obtained by the above method or the extraction polyose IR-960 Fourier Transform Infrared Spectrometer of Radix Astragali
(Chinese Tianjin company), the accumulative scanning of each sample 10 times, when scanning, deducts the interference of air vapor, as shown in Figure 1, Fig. 1
Middle a is the infrared Absorption curve of wolfberry fruit extract, and b is the infrared Absorption curve of Astragalus Root P.E, has in figure a and b simultaneously
Survey and there is related 3382cm- 1、2929cm- 1、1648cm- 1、1412cm- 1、1056cm- 1、829cm- 1It is medium-height grass Deng one
The column characteristic peak of medicine polysaccharide, and peak type is consistent with the peak type of polysaccharide, other peaks are less, thus may determine that being polysaccharide.
Simultaneously by the extraction polyose of fructus lycii obtained by the above method or Radix Astragali Series Molecules amount standard sample HPGFC
Retention time is analyzed, respective distribution coefficient Kav is calculated with retention time and makees abscissa, relative molecular mass logarithm makees ordinate
Obtaining equation of linear regression is y=-1.9789x+5.1021.Polysaccharides GK-1, astragalus polyose GK-2 are surveyed under the same conditions
It is fixed, by sample retention time by calculating, by equation of linear regression, Qi polysaccharide GK-1 is obtained, astragalus polyose GK-2's is opposite
Molecular mass (Mw) is respectively 8161,9124. to have obtained small molecular polysaccharide, and this method has to be obtained under limited working condition
Quick and precisely obtain the small molecular polysaccharide with immune function.
In addition to quickly detection selects the rapid verification detection being integrated to choose pure natural hippophae brass and sea-buckthorn VC,
The present invention also provides a kind of methods that pure sea-buckthorn brass VC detection is chosen, step 1: by therapeutic medical natural hippophae flavones and
Thin crystal light transmitting sheet or solution is made in natural hippophae VC, specifically takes 5-10 Hippophate flavone or sea-buckthorn VC and enough potassium bromide
It is fitted into absorption vessel after grinding or is dissolved in solvent with 5-10g and is packed into absorption vessel and with UV Portable ultraviolet light photometer tests its light transmission
Rate, while being respectively under equal conditions 68% He with the light transmittance that natural standard Hippophate flavone and natural standard sea-buckthorn VC are surveyed
98%, select numerical value percentage of the light transmittance compared with the light transmittance of corresponding standard Hippophate flavone or corresponding standard sea-buckthorn VC
Substance corresponding to not less than 96%, as the medical Hippophate flavone chosen in the first step test in have the same item of flavones a kind of
UV transmittance under part is 61%, then the percentage of itself and standard Hippophate flavone is 66/68*100=97%, and standard
The ratio of Hippophate flavone is greater than 96%, can be used as selection object；Step 2: then by being selected in the above-mentioned first step of electron-microscope scanning
The structure of substance is taken, the scanning electron microscope of preferably SS series obtains its sem image, by it in natural standard Hippophate flavone or day
Right sea-buckthorn VC sem image comparative analysis, as shown in Fig. 2, the right is the mark of natural hippophae flavones and left side natural hippophae VC in figure
Quasi- electron-microscope scanning image is chosen subject image substance corresponding not less than 99.5% image with standard picture similarity, is needed simultaneously
The similarity of the 99% nonbody image and standard picture of wanting the total image area of subject image Zhan is right not above 30% image institute
The comparative analysis of the substance answered, above-mentioned all sem images preferably passes through image analysis software；Step 3: by above-mentioned second step
The sea-buckthorn brass of the selection corresponding infrared spectrum that goes out for carrying out examination of infrared spectrum parallel under sea-buckthorn VC the same terms, selection are husky
Spine brass is consistent with peak type connecting structure at least three of secondary peak in the infrared spectrum in sea-buckthorn VC or spectrogram secondary peak peak type and company
Connecing the corresponding substance of the similar spectrogram of construction is the substance being selected, and described peak type connecting structure at least three unanimously includes following
Characteristic absorption peak 3523cm- 1, 3412cm- 1,3378cm- 1,2927cm- 1,1564cm- 1,1695cm- 1,1631cm- 1,821cm- 1
Occur, at least one peak valley is connected between above-mentioned absorption peak every two, by it is above-mentioned include between homologous plant of the same race may be implemented
The confirmation of heterogeneity interindustry relation and calibrating embodied by the physical chemistry characteristic numeralization of its common excessive substance.Institute
State in third step, further include by the peak on the spectrogram with the ordinate value of peak type curve correspond to length and peak type curve by its
Opening closure after abscissa distance calculate peak type constitute area process, as shown in figure 1 b curve wave number 4000-3000 it
Between peak opening closure after the face of virtual triangle that calculates of the distance of abscissa and the height number of ordinate at the peak 3378
Product, is determined as secondary peak, the peak type for the peak that peak type area value in a spectrum curve is the 0.5-5% of maximum peak type area figures
And the similar size including peak area of connecting structure is in 1% and the number of the feature of peak curvilinear figure and close peak connected to it
Value and graphic feature are close, and close graphic feature includes that shape tendency is close, carry out being described in parallel under described the same terms
Sea-buckthorn is that similar or similar ambient growth conditions sea-buckthorn, the environmental condition tested, parameter are identical.Pass through above selection
Method can fast implement selection and examine other natural hippophae flavones and sea-buckthorn VC, and by autologous correlation compatibility come effectively simple and direct
Realization detect and choose, while preventing the artificial synthesized VC of non-natural and flavonoids object, while it is cumbersome to reduce traditional chemical pick
Step process and stringent duration operating instruction and experiment condition.In addition pure natural sand is chosen in above-mentioned rapid verification detection
The method of spine brass and sea-buckthorn VC can also be used in Radix Salviae Miltiorrhizae or the quick detection of flavones and polysaccharide in Radix Notoginseng is chosen, with this method
Unlike, when testing sample preparation, the interference of alkaloid in order to prevent influences quick pick, need to be by therapeutic medical natural Radix Salviae Miltiorrhizae
Or the sample preparation in faintly acid low temperature environment of Radix Notoginseng flavones and polysaccharide is tested, the preferred pH of the acidic environment is 5-6, by temperature
1-3g glacial acetic acid under the conditions of degree 3-5 degree is mixed with 20-30g potassium bromide to be produced the sampling to be checked of Radix Notoginseng or Radix Salviae Miltiorrhizae and carries out detection choosing
The environmental condition for taking the instrument occurred in above method technique detection to use is synchronization or carries out test point in the state of parallel
Analysis.
The above is only a preferred embodiment of the present invention, for those of ordinary skill in the art, according to the present invention
Thought, there will be changes in the specific implementation manner and application range, and the content of the present specification should not be construed as to the present invention
Limitation.
Claims (4)
1. a kind of Chinese medicine is immune to treat material, it is characterised in that: its composed structure are as follows: including centerbody: it includes nano-magnetics
Grain, the nano magnetic particle are equipped with gap, and the centerbody whole installation includes structural identification digitization measuring mechanism
The substance of function；
It further include around body: corresponding on the surrounding body and centerbody solid arrangement and the two ontology to be equipped with structural knowledge
The substance of other digitization measuring mechanism function, it includes tying on poly- Paederia scandens time glycosides methacrylate polymer nano molecular chain that surrounding body, which is constituted,
Conjunction has Chinese medicine immune substance, and the partial size of the surrounding body is not less than 25 nanometers, and the partial size of the centerbody is not more than 10 nanometers, institute
The quantity ratio for stating the quantity of body around and the centerbody is (80-150): 1, body exists around it when the materials serve acts on
It is distributed or is present in 3 D stereo space surface around centerbody, the corresponding centerbody has the center with body around
Or among the 3 D stereo of surrounding body composition, the polymer nanocomposite strand further includes with acetyl woodruff acid glycosides methyl esters, sweet tea
The polymerization of dish alkali, the molecular weight 10 of the polymer nanocomposite molecule4-106, Chinese medicine immune substance include polysaccharides, asperuloside,
Sea-buckthorn brass, Tanshinone II, danshensu, sea-buckthorn VC, the nano magnetic particle surface are equipped with gap, the nano-magnetic
Grain surface is provided with bismuth manganese Ce-Al alloy or its oxide while the gap being made to constitute coating gap, and the gap of the coating accounts for
The 60%-80% of magnetic particle size, at least provided with basic oxide salt in the coating gap, to provide dynamic body coefficient
According to the material of the detection tumour mechanism of change.
2. material as described in claim 1, it is characterised in that: the magnetic-particle is nano oxidized iron particle, the alkali formula
Oxide salt is percarbonic acid ammonium or SODIUM PERCARBONATE.
3. the manufacturing method of the material as described in any one in claim 1-2, it is characterised in that: received step 1: choosing 6-8
The magnetic-particle of rice, which fills Single port, to be had in the pressure-bearing filter cylinder of 5-7 nanofiltration membrane, is then passed through pressure from the other end of filter cylinder
0.3-0.6Mpa, hydrochloric acid mist 8 to 20 hours of temperature 30-40 degree, containing percentage by volume be 15- in the hydrochloric acid mist
25% nitrogen and vapor, after magnetic-particle is then put into cleaned by ultrasonic vibration drying, then in its electroplating surface or painting
Magnetic-particle after coating, is finally soaked by the bismuth manganese Ce-Al alloy or its oxide coating for connecing one layer 0.5-1 nanometers first
Impregnating high concentration percarbonic acid ammonium salt solution after the SODIUM PERCARBONATE of full high concentration again makes gap fill the flat rear anhydrous formation institute of low-temperature air-drying
The centerbody stated；
Step 2: with 10-20g Paederia scandens time glycosides methyl esters with include being melted with acetyl woodruff acid glycosides methyl esters 5-8g or glycine betaine 3-5g
Enter in 70-100g water, 22-35 DEG C of initiation polymerization, regular oxidation initiator causes, and amount of initiator is aqueous solution weight percent
0.2-0.6%, molecular weight are controlled 103-104Afterwards, Qi polysaccharide, asperuloside, danshensu is added and sea-buckthorn VC, sea-buckthorn is added simultaneously
VC amount is amount of initiator 5/10ths to ten/10th, to adjust the molecular structure and protection immune substance that reaction generates, is risen
It is A liquid that temperature to 38-42 degree, which reacts 10-30 minutes postscripts, then other Chinese medicines including sea-buckthorn brass, Tanshinone II are immunized
Substance is added including in the organic solvent including ethyl acetate, the amount of the organic solvent is 1.1-1.4 times of water, then
35-40 degree postscript is heated with stirring to after the sorbester p17 and Tween20 of the total 0.2-0.6g of weight is added in the organic solvent
For B liquid, B liquid body around the removing organic solvent formation in 15-30 minute of mechanical oscillation emulsion reaction under 35-40 degree is added in A liquid,
The addition quality including Qi polysaccharide, asperuloside, danshensu, sea-buckthorn brass, Tanshinone II is that Immune discrimination structure is adjusted
Object includes Paederia scandens time glycosides methyl esters and includes 80-120 times for using acetyl woodruff acid glycosides methyl esters or betaine polymer quality, institute
The ratio for stating sorbester p17 and the weight of Tween20 is 15-5 than 1, so that surrounding body generates corresponding molecular weight and space structure nanometer
Substance；
Step 3: by the centerbody in the first step and the ratio with the 1:72--150 by mass percentage of the middle surrounding body in second step
Example forms the immune one kind for treating material of the Chinese medicine after mixing and is sealed.
4. the non-diagnostic purpose of material described in any one detects tumour Dynamic Mechanism for digitization in application claim 1-2
Method, it is characterised in that: step 1: suitable experiment mice is selected to be randomly divided into several groups, to its feeding nitrite and
For the mixing food of coal tar until its stomach generates neoplastic lesion, observation test passes through its stomach when tumour growth to diameter 3cm
It includes current potential, pH value, temperature, nitric oxide parameter that sensor is arranged at portion's tumour to test it；
Step 2: the choosing wherein first group and second group radiation for awarding multiple micro- dosage, it is straight that the transformation of its tumor size is observed in test
To the malignant change for generating diameter 1-2cm or start to shift,
Step 3: the gap of Selection Center body magnetic-particle accounts for, magnetic-particle certain volume Chinese medicine is immune to treat material, gives first group
After the immune treatment material of the Chinese medicine of above-mentioned selection in the injection present invention, while being lower than 40 degree by the heating of electromagnetic wave irradiation centerbody
So that percarbonate in its gap is all decomposed and is gone out by decomposing the gas generated, passes through centerbody described in Imaging Study point
It is distributed in tumour cell center, and after surrounding body is distributed in the effect a period of time of the surrounding centered on tumour cell, in its stomach
It includes current potential, pH value, temperature, nitric oxide parameter that sensor is arranged at portion's tumour to test it, in the judgement of sound wave image
Percarbonic acid object in heart body gap decomposes when completing, and be arranged at its tumor stomach sensor test it include current potential, pH value,
Temperature, nitric oxide parameter, and by the size and its tumour Dynamic Mechanism of parameter and Imaging Study judgement record tumour, simultaneously
Determine that the amount and tumour of corresponding basic oxide salt or oxide or percarbonate identify object according to above-mentioned tumour dynamic change
Sensitivity is to instruct detection or Analysis on Mechanism, while as a control group with second group, the corresponding setting at its tumor stomach
It includes current potential, pH value, temperature, nitric oxide parameter that sensor, which tests it, and records corresponding sound wave image parameters, by above-mentioned mistake
The stage situation of parameter information record system chemical conversion data and corresponding reaction kinetic tumor development in journey, for precisely detection control
Dynamic tumor providing method.
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| CN201910658170.1A CN110376137B (en) | 2018-12-18 | 2018-12-18 | Method for detecting and selecting high-purity seabuckthorn flavone and seabuckthorn VC |
| CN201811550340.6A CN109738627B (en) | 2018-12-18 | 2018-12-18 | A kind of immune method for treating material and its digitization detection tumour Dynamic Mechanism of Chinese medicine |
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| CN101121118A (en) * | 2007-04-27 | 2008-02-13 | 天津德欧特生物医药科技有限公司 | Modified macroporous polyvinyl alcohol resin and method for separating and purifying Chinese medicinal herb polysaccharide |
| CN104600358A (en) * | 2015-01-21 | 2015-05-06 | 长沙宝锋能源科技有限公司 | All-solid-state polymer electrolyte for surface chemical grafting inorganic particles and preparation method of all-solid-state polymer electrolyte |
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| CN102188720A (en) * | 2010-03-08 | 2011-09-21 | 大连大学 | Method for studying base of medicinal effect materials |
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| CN110218266A (en) | 2019-09-10 |
| CN110376137B (en) | 2020-12-08 |
| CN109738627A (en) | 2019-05-10 |
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