CN109734936A - A kind of chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion and its preparation - Google Patents
A kind of chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion and its preparation Download PDFInfo
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Abstract
The invention belongs to the technical field of High Internal Phase Emulsion, discloses a kind of chitosan-casein phosphopeptide composite nanometer particle and stablize Pickering High Internal Phase Emulsion and its preparation.The method: 1) chitosan wiring solution-forming is obtained by chitosan solution using aqueous acetic acid；Using water by casein phosphopeptide wiring solution-forming, casein phosphopeptide solution is obtained；2) chitosan solution is mixed with casein phosphopeptide solution, and carries out shearing homogeneous, obtain the system comprising nano particle；3) pH for adjusting the system comprising nano particle is 4~6, then is mixed with grease, emulsification pretreatment, obtains chitosan-casein phosphopeptide composite nanometer particle and stablizes Pickering High Internal Phase Emulsion.Stability of emulsion of the invention is high, and storage time, which reaches, does not occur anti-cream demulsification oil leakage phenomenon for six months or more, and antioxygenic property is good.
Description
Technical field
The present invention relates to a kind of Pickering lotions, and in particular to a kind of chitosan-casein phosphopeptide composite Nano
Grain stablizes Pickering High Internal Phase Emulsion and preparation method thereof.
Background technique
Pickering lotion is a kind of lotion that traditional organic surface active agent stabilising system is replaced by solids, tool
There are many type, such as o/w, w/o or even compound.Its stable mechanism is mainly that solid particle is adsorbed in oil-water interfaces and is formed
Solid particle single-layer or multi-layer film is to stable emulsion.Pickering lotion possesses the lotion stable with conventional surfactant
Identical nature and characteristic, the replacer that can be used as conventional emulsion to a certain extent are applied in each field.Conventional emulsion uses
Surfactant often have certain side effect, the use in fields such as cosmetics, drug, food is restricted.And by
The stable Pickering lotion of solid particle is comparatively safe, less toxic.Therefore Pickering lotion cosmetics, food, pharmacy,
The industries such as petroleum and wastewater treatment have been widely used.
Chitosan CS is a kind of alkaline kation polysaccharide, is obtained by chitin by alkaline deacetylation.Its source is rich
Richness has the characteristics that good biocompatibility, biodegradability, biocidal property have been widely cited in many fields.Casein
The casein phosphopeptide CPP containing phosphorylation serine residue is obtained after trypsin digestion, with a variety of physiological activity
Minerals are such as complexed in function, improve the absorption of Ca, Fe, Zn etc., improve the immunity of the human body, and inhibit cancer cell to increase, as edible
Use antioxidant.
The present invention is by the compound stable structure that is prepared into of positively charged chitosan CS and electronegative casein phosphopeptide CPP
Then nano particle is used to prepare stable Pickering lotion by nano particle.Lotion of the invention is not added with surface-active
Agent, but obtained stability of emulsion is high, storage time is long, and antioxygenic property is good.
Summary of the invention
In order to overcome the shortcomings and deficiencies of the prior art, it is an object of that present invention to provide a kind of chitosan-casein phosphoric acid
Peptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion and preparation method thereof.Fluid oil is transformed by lotion of the invention
Solid oil, and no added surfactant can be still stable for up to six months or more；In thermal acceleration enzymatic oxidation, chitosan-junket
The stable Pickering High Internal Phase Emulsion of protein phosphatase polypeptide composite nanometer particle has good antioxygenic property.
The purpose of the present invention is achieved through the following technical solutions:
A kind of chitosan-casein phosphopeptide composite nanometer particle stablizes the preparation method of Pickering High Internal Phase Emulsion,
The following steps are included:
(1) chitosan wiring solution-forming is obtained chitosan solution (CS solution) using aqueous acetic acid；Using water by junket egg
White phosphoeptide wiring solution-forming obtains casein phosphopeptide solution (CPP solution)；
(2) chitosan solution is mixed with casein phosphopeptide solution, and carries out shearing homogeneous, obtaining includes nano particle
System；
(3) pH for adjusting the system comprising nano particle is 4~6, then is mixed with grease, emulsification pretreatment, and chitosan-is obtained
The stable Pickering High Internal Phase Emulsion of casein phosphopeptide composite nanometer particle.
The mass concentration of aqueous acetic acid described in step (1) is 1%~3%；The dosage of chitosan is acetum matter
The 0.6%~1.2% of amount.
The dosage of casein phosphopeptide described in step (1) is the 0.1%~0.6% of water quality.
The volume ratio of chitosan solution and casein phosphopeptide solution is 1:1 in step (2).
Described in step (2) shear homogeneous revolving speed 3000rpm~6000rpm, shear homogeneous time be 3min~
5min。
Grease described in step (3) is corn oil or linseed oil.
The volume ratio of system and grease that pH is adjusted in step (3) is 1:3~1:5；Emulsification pretreatment described in step (3)
Revolving speed is 8000rpm~12000rpm, and the time of emulsification pretreatment is 30s~80s.
PH described in step (3) is adjusted using sodium hydroxide solution, and the mass concentration of sodium hydroxide solution is 0.5%~
2%.
The chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion and passes through above-mentioned side
Method is prepared.
Compared with prior art, the present invention has the following advantages and beneficial effects:
(1) the present invention is based on chitosan-casein phosphopeptide composite nanometer particles to stablize Pickering High Internal Phase Emulsion,
Fluid oil is successfully changed into solid oil, without any surfactant.
(2) the stable Pickering High Internal Phase Emulsion of chitosan of the invention-casein phosphopeptide composite nanometer particle is steady
Qualitative height, storage time, which reaches, does not occur the oil leakage phenomenon that is demulsified for six months or more.
(3) for stablizing chitosan-casein phosphopeptide composite Nano of Pickering High Internal Phase Emulsion in the present invention
Grain is natural materials, and has a variety of excellent biochemical functions properties, green, natural, health.
(4) the stable Pickering High Internal Phase Emulsion of chitosan of the invention-casein phosphopeptide composite nanometer particle exists
There is good antioxygenic property in thermal acceleration enzymatic oxidation.
Detailed description of the invention
Fig. 1 is that the Pickering High Internal Phase Emulsion that brand-new CS-CP composite nanometer particle is stable in embodiment 1 is inverted appearance
Figure；Respectively be from left to right 0.3%CS-0.05%CPP, 0.3%CS-0.1%CPP, 0.3%CS-0.15%CPP,
0.3%CS-0.2%CPP, 0.3%CS-0.25%CPP, 0.3%CS-0.3%CPP composite nanometer particle are stable
Pickering High Internal Phase Emulsion；
Fig. 2 is that the stable Pickering High Internal Phase Emulsion of CS-CP composite nanometer particle placed in embodiment 16 months falls
Set outside drawing；It respectively is 0.3%CS-0.05%CPP, 0.3%CS-0.1%CPP, 0.3%CS-0.15% from left to right
CPP, 0.3%CS-0.2%CPP, 0.3%CS-0.25%CPP, 0.3%CS-0.3%CPP composite nanometer particle are stable
Pickering High Internal Phase Emulsion；
Fig. 3 is that the Pickering High Internal Phase Emulsion that brand-new 0.3%CS is stable in comparative example 1 is inverted outside drawing；
Fig. 4 is the stable Pickering High Internal Phase Emulsion outside drawing of 0.3%CS placed in comparative example 1 24 hours；
Fig. 5 is in the stable Pickering high of the CS-CPP Nano composite granules of difference CPP mass concentration in embodiment 2
The centrifugation outside drawing of phase emulsion；It respectively is 0.3%CS-0.05%CPP, 0.3%CS-0.1%CPP, 0.3% from left to right
CS-0.15%CPP, 0.3%CS-0.2%CPP, 0.3%CS-0.25%CPP, 0.3%CS-0.3%CPP composite nanometer particle
Outside drawing after stable Pickering High Internal Phase Emulsion centrifugation；
Fig. 6~11 are respectively 0.3%CS-0.05%CP, 0.3%CS-0.1%CP, 0.3%CS-0.15% in embodiment 3
CP, 0.3%CS-0.2%CP, 0.3%CS-0.25%CP, 0.3%CS-0.3%CP composite nanometer particle stablize Pickering
High Internal Phase Emulsion stress scans figure；G' indicates that elasticity modulus, G " indicate sticky mould；
Figure 12 is that 0.3%CS-0.1%CP, 0.3%CS-0.2%CP composite nanometer particle are stable in embodiment 4
Pickering High Internal Phase Emulsion and simple linseed oil are heat-treated hydroperoxides in 10 days greases in 60 DEG C of constant temperature and contain quantitative change
Change curve；
Figure 13 is that 0.3%CS-0.1%CP, 0.3%CS-0.2%CP composite nanometer particle are stable in embodiment 4
Pickering High Internal Phase Emulsion and simple linseed oil are heat-treated mda content in 10 days greases in 60 DEG C of constant temperature and change song
Line.
Specific embodiment
To be best understood from the present invention, the present invention is described further below with reference to embodiment, but implementation of the invention
Mode is without being limited thereto.
Embodiment 1
A kind of chitosan-casein phosphopeptide stablizes the preparation method of Pickering High Internal Phase Emulsion, including following step
It is rapid:
(1) it accurately weighs 0.06g chitosan CS and is dissolved in the acetum that 10ml mass concentration is 1%, it is dense to obtain quality
The CS solution that degree is 0.6%；
(2) 0.02g casein phosphopeptide CPP is dissolved in 10ml ultrapure water, obtains the CPP that mass concentration is 0.2%
Solution；
(3) CS solution obtained by step (1) is added in CPP solution obtained by step (2), is sheared under revolving speed 4000r/min
3min obtains the composite nanometer particle that mass concentration is 0.3%CS-0.1%CPP；
(4) regulating step (3) acquired solution pH value is 5.0；
(5) 1.2ml step (4) acquired solution and 4.8ml corn oil is taken to mix, emulsification pretreatment revolving speed is 8000r/min, when
Between be 60s, obtain chitosan-casein phosphopeptide and stablize Pickering High Internal Phase Emulsion.
The present embodiment has carried out gradient test to the mass concentration of CPP in step (2), mass concentration is respectively as follows: 0.1%,
0.2%, 0.3%, 0.4%, 0.5%, 0.6%, the CS-CP composite nanometer particle for obtaining different CPP mass concentrations is stable
Pickering High Internal Phase Emulsion (is denoted as 0.3%CS-0.05%CP, 0.3%CS-0.1%CP, 0.3%CS-0.15% respectively
CP, 0.3%CS-0.2%CP, 0.3%CS-0.25%CP, 0.3%CS-0.3%CP).Lotion is observed in brand-new and after 6 months
Inversion appearance respectively as shown in (brand-new lotion be inverted outside drawing), Fig. 2 (place 6 months lotions and be inverted outside drawing).Either
In the inversion of brand-new still after six months, High Internal Phase Emulsion forms gel, is changed into solid-state from liquid, and lotion can be with
Bottle is inverted before i.e. inversion in the lower section of bottle, in the top of bottle after inversion.
Comparative example 1
Chitosan stablizes the preparation method of Pickering High Internal Phase Emulsion, comprising the following steps:
(1) it accurately weighs 0.03g chitosan CS and is dissolved in the acetum that 10ml mass concentration is 1%, it is dense to obtain quality
The CS solution that degree is 0.3%；
(2) regulating step (1) acquired solution pH value is 5.0；
(3) 1.2ml step (2) acquired solution and 4.8ml corn oil is taken to mix, emulsification pretreatment revolving speed is 8000r/min, when
Between be 60s, obtain chitosan stablize Pickering High Internal Phase Emulsion.
The lotion of this comparative example preparation carries out inversion experiment in brand-new, is inverted outside drawing and sees Fig. 3；Brand-new 0.3%CS is steady
After fixed Pickering High Internal Phase Emulsion is inverted, lotion is liquid, and inverted front and back lotion is constantly under bottle
Side；It places 24 hours, after the emulsion appearance figure of comparative example preparation is as shown in figure 4, place 24 hours, 0.3%CS is stable
Pickering High Internal Phase Emulsion is demulsified completely.In short, by 0.3%CS when pH value is 5.0 phase in the Pickering high for preparing
Lotion is simultaneously unstable, and the lotion of brand-new can not achieve inversion, and lotion is demulsified completely after placing for 24 hours.
The emulsion types (o/w or w/o) that embodiment 1 is prepared are the dispersion feelings dripped in water and oil by observing lotion
Condition judges.Hair after chitosan-casein phosphopeptide nanoparticles stable Pickering High Internal Phase Emulsion is dripped in water
Existing lotion is dispersible to be opened, and is dripped in oil and is conglobulated, illustrates that the lotion is o/w type lotion, and steady in the emulsion preparation process
Determining agent is chitosan-casein phosphopeptide composite nanometer particle, so lotion of the invention belongs to o/w type Pickering lotion.
By Fig. 1 and Fig. 2 it is found that respectively by 0.3%CS-0.05%CP, 0.3%CS-0.1%CP,
0.3%CS-0.15%CP, 0.3%CS-0.2%CP, 0.3%CS-0.25%CP, 0.3%CS-0.3%CP are in pH
It the Pickering High Internal Phase Emulsion brand-new of nanoparticles stable when value is 5.0 and places storage and monthly is able to achieve down up to 6
It sets, which forms gel, is changed into solid-state from liquid.By Fig. 3 and 4 it is found that being existed in comparative example 1 by 0.3%CS
The Pickering High Internal Phase Emulsion for preparing and unstable when pH value is 5.0, the lotion of brand-new can not achieve inversions, placement for 24 hours
Lotion is demulsified completely afterwards.It can thus be seen that the CS that mass concentration is 0.3% is used alone cannot form when pH value is 5.0
Pickering High Internal Phase Emulsion, and a small amount of CPP is added, stabilization can be prepared using the composite nanometer particle that CS and CPP is formed
Pickering High Internal Phase Emulsion.
Embodiment 2
A kind of chitosan-casein phosphopeptide composite nanometer particle stablizes the preparation method of Pickering High Internal Phase Emulsion,
The following steps are included:
(1) it accurately weighs 0.06g chitosan CS and is dissolved in the acetum that 10ml mass concentration is 1%, it is dense to obtain quality
The CS solution that degree is 0.6%；
(2) 0.02g casein phosphopeptide CPP is dissolved in 10ml ultrapure water, obtains the CPP that mass concentration is 0.2%
Solution；
(3) CS solution obtained by step (1) is added in CPP solution obtained by step (2), is sheared under revolving speed 4000r/min
3min obtains the composite nanometer particle that mass concentration is 0.3%CS-0.1%CP；
(4) regulating step (3) acquired solution pH value is 5.0；
(5) 1.2ml step (4) acquired solution and 4.8ml corn oil is taken to mix, emulsification pretreatment revolving speed is 8000r/min, when
Between be 60s, obtain chitosan-casein phosphopeptide and stablize Pickering High Internal Phase Emulsion.
The present embodiment has carried out gradient test to the mass concentration of CPP in step (2), mass concentration is respectively as follows: 0.1%,
0.2%, 0.3%, 0.4%, 0.5%, 0.6%, the CS-CP composite nanometer particle for obtaining different CPP mass concentrations is stable
Pickering High Internal Phase Emulsion (is denoted as 0.3%CS-0.05%CP, 0.3%CS-0.1%CP, 0.3%CS-0.15% respectively
CP, 0.3%CS-0.2%CP, 0.3%CS-0.25%CP, 0.3%CS-0.3%CP), 1.5ml lotion 10000rpm is taken respectively
Lower centrifugation 10min.
The present embodiment characterizes its centrifugal stability by the state of lotion after observation centrifugation.
Fig. 5 is in the stable Pickering high of the CS-CPP Nano composite granules of difference CPP mass concentration in the present embodiment
The centrifugation outside drawing of phase emulsion.All lotions have elutriation to go out in bottom after being centrifuged 10min under 10000rpm.In addition to 0.3%CS-
The Pickering High Internal Phase Emulsion of 0.15%CP, 0.3%CS-0.2%CP nanoparticles stable is kept other than integrality, remaining
There is oil droplet appearance on lotion surface, shows that the CS-CP composite nanometer particle of different CPP mass concentrations is stable to its
Pickering High Internal Phase Emulsion stability has certain influence.
Embodiment 3
A kind of chitosan-casein phosphopeptide composite nanometer particle stablizes the preparation method of Pickering High Internal Phase Emulsion,
The following steps are included:
(1) it accurately weighs 0.06g chitosan CS and is dissolved in the acetum that 10ml mass concentration is 1%, it is dense to obtain quality
The CS solution that degree is 0.6%；
(2) 0.02g casein phosphopeptide CPP is dissolved in 10ml ultrapure water, obtains the CPP that mass concentration is 0.2%
Solution；
(3) CS solution obtained by step (1) is added in CPP solution obtained by step (2), is sheared under revolving speed 4000r/min
3min obtains the composite nanometer particle that mass concentration is 0.3%CS-0.1%CP；
(4) regulating step (3) acquired solution pH value is 5.0；
(5) 1.2ml step (4) acquired solution and 4.8ml corn oil is taken to mix, emulsification pretreatment revolving speed is 8000r/min, when
Between be 60s, obtain chitosan-casein phosphopeptide and stablize Pickering High Internal Phase Emulsion.1ml lotion is taken to carry out rheology special
Property test, temperature be 25 DEG C, stress scans frequency is set as 1Hz.
The present embodiment has carried out gradient test to the mass concentration of CPP in step (2), mass concentration is respectively as follows: 0.1%,
0.2%, 0.3%, 0.4%, 0.5%, 0.6%, the CS-CP composite nanometer particle for obtaining different CPP mass concentrations is stable
Pickering High Internal Phase Emulsion (is denoted as 0.3%CS-0.05%CP, 0.3%CS-0.1%CP, 0.3%CS-0.15% respectively
CP, 0.3%CS-0.2%CP, 0.3%CS-0.25%CP, 0.3%CS-0.3%CP), take 1ml lotion to carry out rheology respectively
Characteristic test, temperature are 25 DEG C, and stress scans frequency is set as 1Hz, obtains stress scans figure.
Fig. 6~11 are respectively 0.3%CS-0.05%CP, 0.3%CS-0.1%CP, 0.3%CS-0.15%CP, 0.3%
CS-0.2%CP, 0.3%CS-0.25%CP, 0.3%CS-0.3%CP composite nanometer particle are stablized mutually newborn in Pickering high
Liquid stress scans figure.As can be seen from Figure, elastic modulus G ' is greater than viscous modulus G ", shows that High Internal Phase Emulsion under this state is solidifying
The gelling properties based on viscoplasticity are presented in colloidal emulsion.Rheology test shows that chitosan-casein phosphopeptide is stable
Pickering High Internal Phase Emulsion has extraordinary gelling properties, realizes the transformation from liquid fat to solid grease, may replace
Solid grease with trans acids has application value very much, all has in food, medicine field and cosmetic field etc. huge
Application prospect.
Embodiment 4
A kind of chitosan-casein phosphopeptide composite nanometer particle stablizes the preparation method of Pickering High Internal Phase Emulsion,
The following steps are included:
(1) it accurately weighs 0.06g chitosan CS and is dissolved in the acetum that 10ml mass concentration is 1%, it is dense to obtain quality
The CS solution that degree is 0.6%；
(2) 0.02g, 0.04g casein phosphopeptide CPP are dissolved in 10ml ultrapure water respectively, obtaining mass concentration is
0.2%, 0.4% CPP solution；
(3) CS solution obtained by step (1) is added in CPP solution obtained by step (2), is sheared under revolving speed 4000r/min
3min respectively obtains the composite nanometer particle that mass concentration is 0.3%CS-0.1%CP, 0.3%CS-0.2%CP；
(4) regulating step (3) acquired solution pH value is 5.0；
(5) 1.2ml step (4) acquired solution and 4.8ml linseed oil is taken to mix, emulsification pretreatment revolving speed is 8000r/min,
Time is 60s, respectively obtains the stable Pickering of 0.3%CS-0.1%CP, 0.3%CS-0.2%CP composite nanometer particle
High Internal Phase Emulsion.
(6) step (5) Pickering High Internal Phase Emulsion and 4.8ml linseed oil are placed in constant temperature in 60 DEG C of baking ovens and promote oxygen
Change processing 10 days, it is anti-oxidant to probe into the stable Pickering High Internal Phase Emulsion of chitosan-casein phosphopeptide composite nanometer particle
Performance.
Figure 12,13 are respectively that 0.3%CS-0.1%CP, 0.3%CS-0.2%CP composite nanometer particle are stable
Pickering High Internal Phase Emulsion and simple linseed oil 60 DEG C of constant temperature enzymatic oxidations handle hydroperoxides in 10 days greases,
Mda content variation.
Linseed oil contains many unsaturated fatty acids, 60 DEG C of constant temperature enzymatic oxidations 0.3%CS-0.1%CP, 0.3%CS-
0.2%CP composite nanometer particle stable Pickering High Internal Phase Emulsion and simple linseed oil, probe into that CS-CP is compound to be received
The antioxygenic property of the stable High Internal Phase Emulsion of rice grain.Before enzymatic oxidation between 5d each group hydroperoxides content without significance difference
Different, because only surface is exposed to air to pure linseed oil, inside does not have interface, and CS-CP composite nanometer particle is stable
Pickering High Internal Phase Emulsion is in gel state, does not have mobility, and grease is wrapped in inside and prevents partial oxidation.60℃
After constant temperature enzymatic oxidation 5 days, simple linseed oil hydroperoxides and mda content are significantly increased, CS-CP composite nanometer particle
After 10 days 60 DEG C of constant temperature enzymatic oxidations, hydroperoxides, mda content are substantially less than stable Pickering High Internal Phase Emulsion
Simple linseed oil.CPP has certain by the serine residue chelated metal ions containing phosphate group, removing free radical
Antioxygenic property.In addition, CS-CP composite nanometer particle forms interface network barrier in oil droplets, prevent prooxidant with
The contact of grease, inhibits Oxidation of Fat and Oils to a certain extent.Therefore, in the stable Pickering high of CS-CP composite nanometer particle
Phase emulsion imparts a degree of inoxidizability of grease on the transformation basis realized from liquid fat to solid grease
Energy.
Finally, it should be noted that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, to the greatest extent
Pipe has been described in detail the present invention by above preferred embodiment, however, those skilled in the art should understand that, can
To make various changes to it in the form and details, without departing from model defined by claims of the present invention
It encloses.
Claims (8)
1. a kind of chitosan-casein phosphopeptide composite nanometer particle stablizes the preparation method of Pickering High Internal Phase Emulsion,
It is characterized in that: the following steps are included:
(1) chitosan wiring solution-forming is obtained by chitosan solution using aqueous acetic acid；Casein phosphopeptide is made into using water
Solution obtains casein phosphopeptide solution；
(2) chitosan solution is mixed with casein phosphopeptide solution, and carries out shearing homogeneous, obtain the body comprising nano particle
System；
(3) pH for adjusting the system comprising nano particle is 4~6, then is mixed with grease, emulsification pretreatment, and chitosan-junket egg is obtained
White phosphoeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion.
2. chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion according to claim 1
Preparation method, it is characterised in that: the dosage of chitosan described in step (1) be acetum quality 0.6%~1.2%；
The dosage of casein phosphopeptide described in step (1) is the 0.1%~0.6% of water quality；
The volume ratio of chitosan solution and casein phosphopeptide solution is 1:1 in step (2).
3. chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion according to claim 1
Preparation method, it is characterised in that:
Revolving speed 3000rpm~6000rpm that homogeneous is sheared described in step (2), the time for shearing homogeneous is 3min~5min；
Grease described in step (3) is corn oil or linseed oil.
4. chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion according to claim 1
Preparation method, it is characterised in that: the volume ratio of system and grease that pH is adjusted in step (3) is 1:3~1:5.
5. chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion according to claim 1
Preparation method, it is characterised in that: the revolving speed of emulsification pretreatment described in step (3) be 8000rpm~12000rpm, emulsification pretreatment
Time be 30s~80s.
6. chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion according to claim 1
Preparation method, it is characterised in that: the mass concentration of aqueous acetic acid described in step (1) be 1%~3%.
7. a kind of chitosan-casein phosphopeptide composite Nano obtained by any one of claim 1~6 preparation method
Grain stablizes Pickering High Internal Phase Emulsion.
8. chitosan-casein phosphopeptide composite nanometer particle stablizes Pickering High Internal Phase Emulsion according to claim 7
Application in food, medicine field and cosmetic field.
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| CN113116820A (en) * | 2021-04-30 | 2021-07-16 | 中国海洋大学 | Astaxanthin pickering emulsion and preparation method thereof |
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| CN113461968B (en) * | 2021-08-04 | 2024-04-12 | 河南工业大学 | Carboxymethyl chitosan stable high internal phase emulsion and preparation method thereof |
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