CN109734169A - A kind of microbial flocculation method of wheat preparing alcohol waste water - Google Patents
A kind of microbial flocculation method of wheat preparing alcohol waste water Download PDFInfo
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- CN109734169A CN109734169A CN201910057387.7A CN201910057387A CN109734169A CN 109734169 A CN109734169 A CN 109734169A CN 201910057387 A CN201910057387 A CN 201910057387A CN 109734169 A CN109734169 A CN 109734169A
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- culture
- preparing alcohol
- waste water
- flocculant
- klebsiella
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Abstract
The invention discloses the preparations and application of a kind of wheaten starch preparing alcohol wastewater biological flocculant, and the biological flocculant is dedicated for the suspended matter in removal wheaten starch preparing alcohol waste water, the citric acid bacteria strain including being able to produce biological flocculant；Advantages of the present invention are as follows: the biological flocculant has flocculation to wheaten starch preparing alcohol waste water, and tool is significantly reduced the effect of wheaten starch preparing alcohol waste water suspension；Microbial flocculant of the invention has many advantages, such as efficient, safe and nontoxic, biodegradable, without secondary pollution.
Description
Technical field
The present invention relates to a kind of flocculants more particularly to a kind of processing wheaten starch preparing alcohol wastewater biological to wad a quilt with cotton
The preparation method and application of solidifying agent.
Background technique
China is liquor production big country, and numerous as the enterprise of raw material production alcohol using wheat, enterprise produces Alcohol Dregs Wastewater amount
It is huge, and handle difficult.By taking Anhui Ruifuxiang Food Co., Ltd. as an example, it is the raw by raw material of wheat of Largest In China
One of the enterprise of alcohol is produced, produces about 3000 tons of wastewater flow rate daily, COD (Chemical Oxygen Demand, COD) is dense in waste water
Degree is up to 50000mg/L, ss suspended solid (Suspended Solid, SS) 10000mg/L, and total nitrogen concentration reaches 1000mg/L.It is outstanding
Floating object SS main component is total solid 6-7%, protein about 3%, pentosan 1.5-2.5%, yeast thallus 2%-3%, acid
Spend pH 3.2-4.Such waste water has the characteristics that high-temperature, highly acidity, high concentration, high viscosity, suspended particle are tiny.At present
The said firm mainly uses chemical floc --- the process waste water such as polyacrylamide combination air bearing, anaerobic fermentation, polypropylene
Amide dosage about 10-15ppm, polyacrylamide easily remain, and residual monomer acrylamide is the agent of nerve intoxicating, Ke Nengyin
The effect for playing neurotoxicity and cancer shows human body inability, the symptoms such as ataxia after poisoning.Therefore, exploitation safety, height
Effect, " green " flocculant without secondary pollution are particularly necessary.Microbial flocculant becomes because of nontoxic, efficient, " green " environmental protection
Research hotspot in recent years, and have application in the various wastewaters such as food, printing and dyeing, mineral products, dairy products, but have no for wheat
The report of the bioflocculation bacteria research of preparing alcohol waste water, and the specificity of flocculated bacteria is stronger.
Summary of the invention
The present invention in order to overcome the deficiencies of the prior art, provide a kind of wheat preparing alcohol wastewater biological flocculant preparation and
Using new method, to avoid the secondary pollution of chemical flocculation product.
The present invention is achieved by the following technical solutions:
A kind of microbial flocculant, the Klebsiella M-1 bacterial strain including producing biological flocculant.
Described, Klebsiella M-1, Klebsiella sp.M-1 is preserved in Chinese Typical Representative culture on March 1st, 2018
Object collection (abbreviation CCTCC；Address: Wuhan, China, Wuhan University；Postcode: 430072), the culture is in 2018 3
The detection of the moon 15 finishes, and result is survival, and deposit number is CCTCC NO:M2018098, and Klebsiella M-1 bacterium colony is
Obvious wire drawing when transparent, sticky, lawn shape, picking.Gram-positive, rod-shaped, both ends blunt circle has gemma.With generation
The ability of biological flocculant, polyoses content is up to 65% or more in flocculant, protein content only 2%, for typical biology
Flocculant, produced flocculant being capable of efficient flocculating wheaten starch preparing alcohol sewage.
A kind of preparation method of mentioned microorganism flocculant, comprising the following steps:
(1) Klebsiella is seeded in seed culture medium, it is big by 1 × 10 to bacterial strain concentration expands culture8A/above number
Magnitude obtains Klebsiella seed liquor；
(2) seed liquor of step (1) is seeded in fermentation medium, the inoculum concentration of seed liquor is every 100mL fermentation training
Support base be inoculated with 1mL seed liquor, cultivation temperature be 30 DEG C, culture pH be 5, culture revolving speed be 150r/min, incubation time 48h,
Obtain the fermentation liquid with flocculation activity.
(3) seed culture medium compositing formula described in step (2): glucose 20g, yeast extract 0.5g, K2HPO4 5.0g、
KH2PO4 2.0g、 MgSO40.2g, NaCl 0.1g, ammonium sulfate 0.1g, urea 0.5g；Fermentation medium group of formula are as follows: by weight
Meter, main composition are as follows: glucose 15g/L, peptone 2g/L, KH2PO41g/L, K2HPO42.5g/L, NaCl 0.1g；
The temperature of the culture of step (1) or step (2) is 30 DEG C, and the time of culture is 24-48 hours, pH5.
The present invention has the advantage that the present invention provides a kind of processing wheaten starch preparing alcohol waste water compared with prior art
The preparation of biological flocculant and application method, the biological flocculant have flocculation to wheat Alcohol Dregs Wastewater, can reduce
Concentration of suspension in wheat preparing alcohol lees wastewater, concentration of suspension have dropped 80.2%, have significant flocculating effect.This
The flocculant preparation method of invention is simple, and effect is good, at low cost, does not generate any environmental pollution.
Specific embodiment
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
The present embodiment it is a kind of for removing the biological flocculant of hoggery sewerage, preparation method includes following step
It is rapid:
(1) Klebsiella M-1 is first activated, is then seeded in seed culture medium, expand culture extremely at 30 DEG C
Bacterial strain concentration is up to 1 × 108A/ml or more order of magnitude obtains the seed liquor of Klebsiella M-1；
(2) seed liquor of step (1) is seeded in fermentation medium respectively, inoculum concentration is every 100ml fermentation medium
It is inoculated with the seed liquor of 1ml, fermentation culture conditions are as follows: 30 DEG C of temperature, pH5, cultivate revolving speed 150r/min, culture 48h obtains biology
Flocculant；
(3) by weight, the formula of the seed culture medium of above-mentioned steps (1) are as follows: glucose 20g, yeast extract 0.5g,
K2HPO4 5.0g、KH2PO4 2.0g、MgSO40.2g, NaCl 0.1g, ammonium sulfate 0.1g, urea 0.5g；The fermentation of step (2)
The formula of culture medium are as follows: main composition is as follows: glucose 15g/L, peptone 2g/L, KH2PO41g/L, K2HPO42.5g/L
NaCl 0.1g；
(4) according to the kind of 8% addition Klebsiella M-1 of wheaten starch preparing alcohol grain liquid first time centrifuge waste water weight
Sub- liquid stands 30 minutes after stirring 3min.One layer of limpid hyaline layer is presented above at this time, is purified through M1 fermented liquid
Treated SS, COD, BOD and turbidity compared with being significantly improved before purified treatment, removal rate respectively reaches 71.9%,
61.5%, 48.6% and 72.7%.
Claims (2)
1. a kind of microbial flocculation of wheat preparing alcohol waste water, which is characterized in that being capable of the preparing alcohol dirt of flocculation sedimentation wheaten starch
The microbial flocculant of aqueous suspension, microbial flocculant is fermented by citric acid bacteria strain M-1 to be generated,
The Klebsiella M-1, Klebsiella sp.M-1, biological deposits number are CCTCC NO:M 2018098.
2. a kind of preparation method of microbial flocculant described in claim 1, which comprises the following steps:
(1) by Klebsiella strain inoculated into seed culture medium, it is big by 1 × 10 to bacterial strain concentration to expand culture8A/above number
Magnitude obtains bacillus amyloliquefaciens seed liquor；
(2) seed liquor of step (1) is seeded in fermentation medium, the inoculum concentration of seed liquor is every 100mL fermentation medium
It is inoculated with 1mL seed liquor, cultivation temperature is 30 DEG C, and culture pH is 5, and culture revolving speed is 150r/min, incubation time 48h, is obtained
Fermentation with flocculation activity；
(3) fermentation medium group of formula described in step (2) are as follows: by weight, glucose 15g/L, peptone 2g/L, KH2PO4
1g/L, K2HPO42.5g/L, NaCl 0.1g；The temperature of the culture of step (1) or step (2) is 30 DEG C, and the time of culture is
24-48 hours, pH 5.
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| CN201910057387.7A CN109734169A (en) | 2019-01-22 | 2019-01-22 | A kind of microbial flocculation method of wheat preparing alcohol waste water |
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| CN201910057387.7A CN109734169A (en) | 2019-01-22 | 2019-01-22 | A kind of microbial flocculation method of wheat preparing alcohol waste water |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176877A (en) * | 2015-10-12 | 2015-12-23 | 青岛美能达生物科技有限公司 | Klebsiella and method for preparing microbial flocculant by using same |
| CN107794238A (en) * | 2017-11-13 | 2018-03-13 | 中国科学院成都生物研究所 | The bacterial strains of BFX 01 of high yield biological flocculant and the biological flocculant of acquisition |
| US20180312850A1 (en) * | 2014-07-03 | 2018-11-01 | Ut-Battelle, Llc | Tnt cloning system |
-
2019
- 2019-01-22 CN CN201910057387.7A patent/CN109734169A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180312850A1 (en) * | 2014-07-03 | 2018-11-01 | Ut-Battelle, Llc | Tnt cloning system |
| CN105176877A (en) * | 2015-10-12 | 2015-12-23 | 青岛美能达生物科技有限公司 | Klebsiella and method for preparing microbial flocculant by using same |
| CN107794238A (en) * | 2017-11-13 | 2018-03-13 | 中国科学院成都生物研究所 | The bacterial strains of BFX 01 of high yield biological flocculant and the biological flocculant of acquisition |
Non-Patent Citations (2)
| Title |
|---|
| DIAO, H: "Screening of High-performance Flocculant-producing Bacteria and Optimization of the Conditions for Flocculation of Wheat Distillery Wastewater", 《BIORESOURCES》 * |
| 吴金鹏: "《食品微生物学》", 31 October 1992, 农业出版社 * |
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