CN109717870A - The multi-modal MR imaging method of Autologous Chondrocyte transplant patient under 3.0T field strength - Google Patents
The multi-modal MR imaging method of Autologous Chondrocyte transplant patient under 3.0T field strength Download PDFInfo
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- CN109717870A CN109717870A CN201811592921.6A CN201811592921A CN109717870A CN 109717870 A CN109717870 A CN 109717870A CN 201811592921 A CN201811592921 A CN 201811592921A CN 109717870 A CN109717870 A CN 109717870A
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Abstract
The invention discloses the multi-modal MR imaging methods of Autologous Chondrocyte transplant patient under 3.0T field strength, method includes the following steps: S1: cultivating Autologous Chondrocyte, S2: patient is anaesthetized, S3: the transplanting of Autologous Chondrocyte is carried out to patient, S4: the scanning of structure picture is carried out to patient, S5: diffusion tensor scanning is carried out to patient, S6: proton MR spectroscopy study scanning is carried out to patient, compared with prior art, the beneficial effects of the present invention are: the present invention first cultivates Autologous Chondrocyte, then Autologous Chondrocyte is implanted in the position that cartilage cell with patient is accordingly damaged, the multi-modal magnetic resonance imaging of the realization Autologous Chondrocyte transplant patient under 3.0T field strength can be passed through.
Description
Technical field
The present invention relates to multi-modal MR imaging method fields, specially Autologous Chondrocyte transplantation disease under 3.0T field strength
The multi-modal MR imaging method of people.
Background technique
Currently, 3.0T magnetic resonance scanner has been widely applied, possess preferable soft tissue resolution, can use well
In the research and diagnosis of central nervous system disease, but at present, the country is not transplanted to Autologous Chondrocyte under 3.0T field strength
Multi-modal MR imaging method technology after with patient, so that domestic diagnosis and research for central nervous system disease
There are a qualitative technical problems.
Summary of the invention
The purpose of the present invention is to provide the multi-modal magnetic resonance imagings of Autologous Chondrocyte transplant patient under 3.0T field strength
Method, to solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme: Autologous Chondrocyte transplant patient under 3.0T field strength
Multi-modal MR imaging method, method includes the following steps:
S1: cultivating Autologous Chondrocyte, after self cartilage is digested with TI Collagen Type VI, is carried out with CM culture medium former
It is commissioned to train feeding, after the cell after originally culture is with trypsin digestion, is resuspended with sodium alginate soln, the cell of resuspension is connected to bright
Glue coated culture vessel, adds CaCl2 solution by 0-4 DEG C after placement 4-8 minutes, 0-4 DEG C placements 4-8 minute, formation cell coagulates
Cellular gels are transferred to the coated culture vessel of gelatin by glue, with CC culture medium culture 15-30 days, every 2-3 days in incubation
Fresh culture is replaced, Autologous Chondrocyte is obtained；
S2: anaesthetizing patient, and patient is placed on seat by postanesthetic patient using prone position；
S3: the transplanting of Autologous Chondrocyte is carried out to patient, Autologous Chondrocyte cultured in S1 is transplanted to patient
With；
S4: carrying out the scanning of structure picture to patient, and fast acquisition interleaved spin echo is first selected to carry out routine to the cranium brain of patient
Cross section T2WI scanning, sagittal plane T2WI scanning obtain axle position structural images, sagittal plain structural images, then expand wide-field hat
Shape face T2WI scanning, obtains and expands wide-field coronal bit image, carries out the scanning of brain functional mri to patient；
S5: carrying out diffusion tensor scanning to patient, and wherein disperse sensitising gradient is 32 different directions；
S6: carrying out proton MR spectroscopy study scanning to patient, be included in expand wide-field coronal bit image determine hippocampus and
Thalamus maximum level and as scanning center's level, the centre of the center level is region of interest, then to rat model
Hippocampus and the acquisition of thalamus row two dimension multi-voxel proton pop.
Preferably, in the step S1, TI Collagen Type VI is powdered collagen, and TI Collagen Type VI adds when digesting self cartilage
Some water are for needed for enzymatic hydrolysis.
Preferably, in the step S3, when autologous transplanting cartilage cell, the Autologous Chondrocyte in S1 is transplanted to patient
The impaired position of cartilage.
Preferably, in the S5, the design parameter of diffusion tensor scanning is TR=3300ms, TE=86ms, FOV=
41mm*41mm, acquisition matrix 130*130. sweep 18 layers altogether, and b value is respectively 0 and 1000s/mm2 in diffusion-weighted coefficient.
Preferably, in the S6, proton MR spectroscopy study scanning, which is used, to be carried out assessing MRS in body in frequency domain, carries out frequency
The resolution ratio for needing to increase by zero insertion processing image when the assessment in domain filters by digitlization and improves signal-to-noise ratio, phase tune
It is whole to obtain pure echo absorption signal, the signal of artifact and fixed member contribution is removed by baseline correction, passes through mathematical model
Carry out parameter fitting, spectrum peak position, area under peak.
Compared with prior art, the beneficial effects of the present invention are: the present invention first cultivates Autologous Chondrocyte, then
Autologous Chondrocyte is implanted in the position that cartilage cell with patient is accordingly damaged, it can be self by being realized under 3.0T field strength
The multi-modal magnetic resonance imaging of chondrocyte cell transplantation patient.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment one
The present invention provides a kind of technical solution: under 3.0T field strength the multi-modal magnetic resonance of Autologous Chondrocyte transplant patient at
Image space method, method includes the following steps:
S1: cultivating Autologous Chondrocyte, after self cartilage is digested with TI Collagen Type VI, is carried out with CM culture medium former
It is commissioned to train feeding, after the cell after originally culture is with trypsin digestion, is resuspended with sodium alginate soln, the cell of resuspension is connected to bright
The coated culture vessel of glue after 0 DEG C is placed 4 minutes, adds CaCl2 solution, and 0 DEG C is placed 4 minutes, and Cellular gels are formed, will be thin
Born of the same parents' gel is transferred to the coated culture vessel of gelatin, with CC culture medium culture 15 days, every 2 days replacement fresh cultureds in incubation
Base obtains Autologous Chondrocyte；
S2: anaesthetizing patient, and patient is placed on seat by postanesthetic patient using prone position；
S3: the transplanting of Autologous Chondrocyte is carried out to patient, Autologous Chondrocyte cultured in S1 is transplanted to patient
With；
S4: carrying out the scanning of structure picture to patient, and fast acquisition interleaved spin echo is first selected to carry out routine to the cranium brain of patient
Cross section T2WI scanning, sagittal plane T2WI scanning obtain axle position structural images, sagittal plain structural images, then expand wide-field hat
Shape face T2WI scanning, obtains and expands wide-field coronal bit image, carries out the scanning of brain functional mri to patient；
S5: carrying out diffusion tensor scanning to patient, and wherein disperse sensitising gradient is 32 different directions；
S6: carrying out proton MR spectroscopy study scanning to patient, be included in expand wide-field coronal bit image determine hippocampus and
Thalamus maximum level and as scanning center's level, the centre of the center level is region of interest, then to rat model
Hippocampus and the acquisition of thalamus row two dimension multi-voxel proton pop.
Wherein, in step S1, TI Collagen Type VI is powdered collagen, and TI Collagen Type VI adds some water when digesting self cartilage
For needed for enzymatic hydrolysis.
Wherein, in step S3, when autologous transplanting cartilage cell, by the Autologous Chondrocyte in S1 be transplanted to patient's cartilage by
The position of damage.
Wherein, in S5, the design parameter of diffusion tensor scanning is TR=3300ms, TE=86ms, FOV=41mm*
41mm, acquisition matrix 130*130. sweep 18 layers altogether, and b value is respectively 0 and 1000s/mm2 in diffusion-weighted coefficient.
Wherein, in S6, proton MR spectroscopy study scanning, which is used, to be carried out assessing MRS in body in frequency domain, carries out commenting for frequency domain
The resolution ratio for needing to increase by zero insertion processing image when estimating, filters by digitlization and improves signal-to-noise ratio, and phase adjustment obtains
Pure echo absorption signal is removed the signal of artifact and fixed member contribution by baseline correction, is joined by mathematical model
Number is fitted, spectrum peak position, area under peak.
Embodiment two
The present invention provides a kind of technical solution: under 3.0T field strength the multi-modal magnetic resonance of Autologous Chondrocyte transplant patient at
Image space method, method includes the following steps:
S1: cultivating Autologous Chondrocyte, after self cartilage is digested with TI Collagen Type VI, is carried out with CM culture medium former
It is commissioned to train feeding, after the cell after originally culture is with trypsin digestion, is resuspended with sodium alginate soln, the cell of resuspension is connected to bright
The coated culture vessel of glue, 2 DEG C are placed after five minutes, and CaCl2 solution is added, and 2 DEG C are placed 5 minutes, and Cellular gels are formed, will be thin
Born of the same parents' gel is transferred to the coated culture vessel of gelatin, with CC culture medium culture 20 days, every 3 days replacement fresh cultureds in incubation
Base obtains Autologous Chondrocyte；
S2: anaesthetizing patient, and patient is placed on seat by postanesthetic patient using prone position；
S3: the transplanting of Autologous Chondrocyte is carried out to patient, Autologous Chondrocyte cultured in S1 is transplanted to patient
With；
S4: carrying out the scanning of structure picture to patient, and fast acquisition interleaved spin echo is first selected to carry out routine to the cranium brain of patient
Cross section T2WI scanning, sagittal plane T2WI scanning obtain axle position structural images, sagittal plain structural images, then expand wide-field hat
Shape face T2WI scanning, obtains and expands wide-field coronal bit image, carries out the scanning of brain functional mri to patient；
S5: carrying out diffusion tensor scanning to patient, and wherein disperse sensitising gradient is 32 different directions；
S6: carrying out proton MR spectroscopy study scanning to patient, be included in expand wide-field coronal bit image determine hippocampus and
Thalamus maximum level and as scanning center's level, the centre of the center level is region of interest, then to rat model
Hippocampus and the acquisition of thalamus row two dimension multi-voxel proton pop.
Wherein, in step S1, TI Collagen Type VI is powdered collagen, and TI Collagen Type VI adds some water when digesting self cartilage
For needed for enzymatic hydrolysis.
Wherein, in step S3, when autologous transplanting cartilage cell, by the Autologous Chondrocyte in S1 be transplanted to patient's cartilage by
The position of damage.
Wherein, in S5, the design parameter of diffusion tensor scanning is TR=3300ms, TE=86ms, FOV=41mm*
41mm, acquisition matrix 130*130. sweep 18 layers altogether, and b value is respectively 0 and 1000s/mm2 in diffusion-weighted coefficient.
Wherein, in S6, proton MR spectroscopy study scanning, which is used, to be carried out assessing MRS in body in frequency domain, carries out commenting for frequency domain
The resolution ratio for needing to increase by zero insertion processing image when estimating, filters by digitlization and improves signal-to-noise ratio, and phase adjustment obtains
Pure echo absorption signal is removed the signal of artifact and fixed member contribution by baseline correction, is joined by mathematical model
Number is fitted, spectrum peak position, area under peak.
Embodiment three
The present invention provides a kind of technical solution: under 3.0T field strength the multi-modal magnetic resonance of Autologous Chondrocyte transplant patient at
Image space method, method includes the following steps:
S1: cultivating Autologous Chondrocyte, after self cartilage is digested with TI Collagen Type VI, is carried out with CM culture medium former
It is commissioned to train feeding, after the cell after originally culture is with trypsin digestion, is resuspended with sodium alginate soln, the cell of resuspension is connected to bright
The coated culture vessel of glue after 3 DEG C are placed 6 minutes, adds CaCl2 solution, and 3 DEG C are placed 6 minutes, and Cellular gels are formed, will be thin
Born of the same parents' gel is transferred to the coated culture vessel of gelatin, with CC culture medium culture 25 days, every 2 days replacement fresh cultureds in incubation
Base obtains Autologous Chondrocyte；
S2: anaesthetizing patient, and patient is placed on seat by postanesthetic patient using prone position；
S3: the transplanting of Autologous Chondrocyte is carried out to patient, Autologous Chondrocyte cultured in S1 is transplanted to patient
With；
S4: carrying out the scanning of structure picture to patient, and fast acquisition interleaved spin echo is first selected to carry out routine to the cranium brain of patient
Cross section T2WI scanning, sagittal plane T2WI scanning obtain axle position structural images, sagittal plain structural images, then expand wide-field hat
Shape face T2WI scanning, obtains and expands wide-field coronal bit image, carries out the scanning of brain functional mri to patient；
S5: carrying out diffusion tensor scanning to patient, and wherein disperse sensitising gradient is 32 different directions；
S6: carrying out proton MR spectroscopy study scanning to patient, be included in expand wide-field coronal bit image determine hippocampus and
Thalamus maximum level and as scanning center's level, the centre of the center level is region of interest, then to rat model
Hippocampus and the acquisition of thalamus row two dimension multi-voxel proton pop.
Wherein, in step S1, TI Collagen Type VI is powdered collagen, and TI Collagen Type VI adds some water when digesting self cartilage
For needed for enzymatic hydrolysis.
Wherein, in step S3, when autologous transplanting cartilage cell, by the Autologous Chondrocyte in S1 be transplanted to patient's cartilage by
The position of damage.
Wherein, in S5, the design parameter of diffusion tensor scanning is TR=3300ms, TE=86ms, FOV=41mm*
41mm, acquisition matrix 130*130. sweep 18 layers altogether, and b value is respectively 0 and 1000s/mm2 in diffusion-weighted coefficient.
Wherein, in S6, proton MR spectroscopy study scanning, which is used, to be carried out assessing MRS in body in frequency domain, carries out commenting for frequency domain
The resolution ratio for needing to increase by zero insertion processing image when estimating, filters by digitlization and improves signal-to-noise ratio, and phase adjustment obtains
Pure echo absorption signal is removed the signal of artifact and fixed member contribution by baseline correction, is joined by mathematical model
Number is fitted, spectrum peak position, area under peak.
Example IV
The present invention provides a kind of technical solution: under 3.0T field strength the multi-modal magnetic resonance of Autologous Chondrocyte transplant patient at
Image space method, method includes the following steps:
S1: cultivating Autologous Chondrocyte, after self cartilage is digested with TI Collagen Type VI, is carried out with CM culture medium former
It is commissioned to train feeding, after the cell after originally culture is with trypsin digestion, is resuspended with sodium alginate soln, the cell of resuspension is connected to bright
The coated culture vessel of glue after 4 DEG C are placed 8 minutes, adds CaCl2 solution, and 4 DEG C are placed 8 minutes, and Cellular gels are formed, will be thin
Born of the same parents' gel is transferred to the coated culture vessel of gelatin, with CC culture medium culture 30 days, every 3 days replacement fresh cultureds in incubation
Base obtains Autologous Chondrocyte；
S2: anaesthetizing patient, and patient is placed on seat by postanesthetic patient using prone position；
S3: the transplanting of Autologous Chondrocyte is carried out to patient, Autologous Chondrocyte cultured in S1 is transplanted to patient
With；
S4: carrying out the scanning of structure picture to patient, and fast acquisition interleaved spin echo is first selected to carry out routine to the cranium brain of patient
Cross section T2WI scanning, sagittal plane T2WI scanning obtain axle position structural images, sagittal plain structural images, then expand wide-field hat
Shape face T2WI scanning, obtains and expands wide-field coronal bit image, carries out the scanning of brain functional mri to patient；
S5: carrying out diffusion tensor scanning to patient, and wherein disperse sensitising gradient is 32 different directions；
S6: carrying out proton MR spectroscopy study scanning to patient, be included in expand wide-field coronal bit image determine hippocampus and
Thalamus maximum level and as scanning center's level, the centre of the center level is region of interest, then to rat model
Hippocampus and the acquisition of thalamus row two dimension multi-voxel proton pop.
Wherein, in step S1, TI Collagen Type VI is powdered collagen, and TI Collagen Type VI adds some water when digesting self cartilage
For needed for enzymatic hydrolysis.
Wherein, in step S3, when autologous transplanting cartilage cell, by the Autologous Chondrocyte in S1 be transplanted to patient's cartilage by
The position of damage.
Wherein, in S5, the design parameter of diffusion tensor scanning is TR=3300ms, TE=86ms, FOV=41mm*
41mm, acquisition matrix 130*130. sweep 18 layers altogether, and b value is respectively 0 and 1000s/mm2 in diffusion-weighted coefficient.
Wherein, in S6, proton MR spectroscopy study scanning, which is used, to be carried out assessing MRS in body in frequency domain, carries out commenting for frequency domain
The resolution ratio for needing to increase by zero insertion processing image when estimating, filters by digitlization and improves signal-to-noise ratio, and phase adjustment obtains
Pure echo absorption signal is removed the signal of artifact and fixed member contribution by baseline correction, is joined by mathematical model
Number is fitted, spectrum peak position, area under peak.
The present invention first cultivates Autologous Chondrocyte, and it is thin that Autologous Chondrocyte is then implanted in cartilage with patient
The position that cell phase should be damaged, can by under 3.0T field strength realize Autologous Chondrocyte transplant patient multi-modal magnetic resonance at
Picture.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1.3.0T under field strength Autologous Chondrocyte transplant patient multi-modal MR imaging method, it is characterised in that: this method
The following steps are included:
S1: cultivating Autologous Chondrocyte, after self cartilage is digested with TI Collagen Type VI, carries out primary training with CM culture medium
It supports, after the cell after originally culture is with trypsin digestion, is resuspended with sodium alginate soln, the cell of resuspension is connected to gelatin packet
The culture vessel of quilt, adds CaCl2 solution by 0-4 DEG C after placement 4-8 minutes, 0-4 DEG C placements 4-8 minutes, formation Cellular gels,
Cellular gels are transferred to the coated culture vessel of gelatin, every 2-3 days more with CC culture medium culture 15-30 days, in incubation
Fresh culture is changed, Autologous Chondrocyte is obtained；
S2: anaesthetizing patient, and patient is placed on seat by postanesthetic patient using prone position；
S3: the transplanting of Autologous Chondrocyte is carried out to patient, Autologous Chondrocyte cultured in S1 is transplanted to patient；
S4: carrying out the scanning of structure picture to patient, and fast acquisition interleaved spin echo is first selected to carry out the transversal of routine to the cranium brain of patient
Face T2WI scanning, sagittal plane T2WI scanning obtain axle position structural images, sagittal plain structural images, then expand wide-field coronal-plane
T2WI scanning, obtains and expands wide-field coronal bit image, carries out the scanning of brain functional mri to patient；
S5: carrying out diffusion tensor scanning to patient, and wherein disperse sensitising gradient is 32 different directions；
S6: carrying out proton MR spectroscopy study scanning to patient, is included in the wide-field coronal bit image of expansion and determines hippocampus and thalamus
Maximum level and as scanning center's level, the centre of the center level is region of interest, then to the hippocampus of rat model
And thalamus row two dimension multi-voxel proton pop acquisition.
2. the multi-modal magnetic resonance imaging side of Autologous Chondrocyte transplant patient under 3.0T field strength according to claim 1
Method, it is characterised in that: in the step S1, TI Collagen Type VI is powdered collagen, and TI Collagen Type VI adds when digesting self cartilage
Some water are for needed for enzymatic hydrolysis.
3. the multi-modal magnetic resonance imaging side of Autologous Chondrocyte transplant patient under 3.0T field strength according to claim 1
Method, it is characterised in that: in the step S3, when autologous transplanting cartilage cell, the Autologous Chondrocyte in S1 is transplanted to patient
The impaired position of cartilage.
4. the multi-modal magnetic resonance imaging side of Autologous Chondrocyte transplant patient under 3.0T field strength according to claim 1
Method, it is characterised in that: in the S5, the design parameter of diffusion tensor scanning is TR=3300ms, TE=86ms, FOV=
41mm*41mm, acquisition matrix 130*130. sweep 18 layers altogether, and b value is respectively 0 and 1000s/mm2 in diffusion-weighted coefficient.
5. the multi-modal magnetic resonance imaging side of Autologous Chondrocyte transplant patient under 3.0T field strength according to claim 1
Method, it is characterised in that: in the S6, proton MR spectroscopy study scanning, which is used, to be carried out assessing MRS in body in frequency domain, carries out frequency domain
Assessment when need to increase by zero insertion processing the resolution ratio of image, filtered by digitlization and improve signal-to-noise ratio, phase adjustment
Obtain pure echo absorption signal, by baseline correction remove artifact and fixed member contribution signal, by mathematical model into
Row parameter fitting, spectrum peak position, area under peak.
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| CN111598864A (en) * | 2020-05-14 | 2020-08-28 | 北京工业大学 | Hepatocellular carcinoma differentiation assessment method based on multi-modal image contribution fusion |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111598864A (en) * | 2020-05-14 | 2020-08-28 | 北京工业大学 | Hepatocellular carcinoma differentiation assessment method based on multi-modal image contribution fusion |
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Application publication date: 20190507 |