CN101361659A - Cynomolgus monkey magnetic resonance scanning method - Google Patents
Cynomolgus monkey magnetic resonance scanning method Download PDFInfo
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- CN101361659A CN101361659A CNA2008102226399A CN200810222639A CN101361659A CN 101361659 A CN101361659 A CN 101361659A CN A2008102226399 A CNA2008102226399 A CN A2008102226399A CN 200810222639 A CN200810222639 A CN 200810222639A CN 101361659 A CN101361659 A CN 101361659A
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Abstract
The invention aims at improving cynomolgus magnetic resonance scanning methods and consequently providing legible brain magnetic resonance images. The invention provides a cynomolgus magnetic resonance scanning method that utilizes a Philips Intera A chiva 3.0T magnetic resonance machine for scanning, a T1 scanning sequence is IR with the visual field of 120mm, the TR/TE of 2153ms/13ms, the seam thickness/interval of 3mm/0.3mm and the matrix of 256 multiplied by 256, while a T2 weighted image scanning sequence is SE with the visual field of 12cm multiplied by 12cm, the TR/TE of 3200ms/102ms, the seam thickness/interval of 3mm/0.3mm and the matrix of 256 multiplied by 256. The cynomolgus magnetic resonance scanning method can provide legible brain magnetic resonance images, and has greater significance in the displaying of cynomolgus craniocerebral anatomical structure and cytological marking and magnetic resonance tracing after brain transplantation.
Description
Technical field
The present invention relates to cynomolgus monkey magnetic resonance scanning method.Need to prove that the present invention does not belong to the diagnostic method of disease, but a kind of method that presents laboratory animal cranium brain anatomical structure or experiment labelling.
Background technology
The cardinal principle of MRI (magnetic resonance imaging) is that radio wave accepter that the magnetic field that utilizes magnetic resonance machine to produce makes health to be placed detects the tiny signal from body interior, and computer transforms these tiny signals, thereby obtains image.The effective imaging time of MRI is long, but the dynamic migration course of observation of cell, space, temporal resolution height, good contrast, so in the live body spike promise well.The live body spike is one of the most effective means in the modern medicine study, can pass through the MRI imaging, the laboratory animal of live body spike is carried out direct observation, thereby obtain the most directly pathological data.In the described laboratory animal, primates and people's physiological feature is the most similar, and machin is the most frequently used a kind of.Yet, after being provided with according to the common operation parameter of existing magnetic resonance machine, after machin scanned, can not form the image that effectively can distinguish.
Summary of the invention
The objective of the invention is to improve cynomolgus monkey magnetic resonance scanning method, thereby brain magnetic resonance image (MRI) clearly can be provided.
Cynomolgus monkey magnetic resonance scanning method provided by the invention scans for utilizing Philips Intera Achiva3.0T magnetic resonance machine, the T1 scanning sequence is IR, the visual field: 120mm, TR/TE:2153ms/13ms, bed thickness/at interval: 3mm/0.3mm, matrix: 256 * 256; The T2 weighting is SE as scanning sequence, the visual field: 12cm * 12cm, TR/TE:3200ms/102ms, bed thickness/spacing: 3mm/0.3mm, matrix: 256 * 256.
Need before scanning machin is anaesthetized, used anesthetis can be ketamine and the new II mixed liquor of speed dormancy of 1:3 for volume ratio, and the consumption of described mixed liquor is about 0.1mL/kg, promptly every kg body weight medication 0.1mL.
Utilize cynomolgus monkey magnetic resonance scanning method provided by the invention, brain magnetic resonance image (MRI) clearly can be provided, the magnetic resonance spike has big meaning after showing machin cranium brain anatomical structure and cell marking intracerebral transplantation.
Description of drawings
Fig. 1 is the crown position of a normal cynomolgus monkey magnetic resonance T1WI image;
Fig. 2 is a normal cynomolgus monkey magnetic resonance axle position T2WI image.
Among the figure: 1-caudatum (caudate nucleus); 2-shell nuclear (putamen); 3-dorsal thalamus (dorsalthalamus); 4-anterior limb of internal capsule (anterior limb of internal capsule); 5-posterior limb of internal capsule (posterior limb of internal capsule); 6-island leaf (insula); 7-tricorn (lateralventricle); 8-corpus callosum (corpus callosum).
The specific embodiment
Machin, male, about 3.5 years old, the about 4.2kg of body weight was provided by Military Medical Science Institute's Experimental Animal Center.Regular grade, credit number: SCXK (army) 2002-001.Entering animal housing quarantined for 2 weeks.Animal Lab. is the non-rodent facility of Institute of Experimental Animals, Chinese Academy of Medical Sciences, regular grade environment, credit number: SYXK (capital) 2005-0042, temperature 18-28 ℃, relative humidity: 40-70%, mechanical supply and exhaust, illumination 150-300Lux, lighting hours 12h/12h.The cage tool carries out disinfection before input, and single cage is raised.Morning and afternoon every day, each was reinforced once, and give fruit once every day.One-level monkey material, regular grade, supplier is a Test Animal Centre, Academy of Military Medical Sciences, P.L.A, quality certification SCXK-(army) 2002-018.Ad lib, the municipal pipeline tap water, automatic water-drinking system is freely drunk water.
Agents useful for same is as follows
The magnetic resonance imaging operational approach is as follows:
Anesthesia: ketamine/new II of speed dormancy presses 1:3 and mixes back 0.1mL/kg, intramuscular injection.
Philips Intera Achiva 3.0T magnetic resonance machine, 8 passage head coils are adopted in scanning.With being fixed on the headstock of exempting from magnetic after the anesthesia of machin monkey, insert in the head coil.The T1 scanning sequence is IR, the visual field: 120mm, and TR/TE:2153ms/13ms, bed thickness/at interval: 3mm/0.3mm, matrix: 256 * 256.The T2 weighting is SE as scanning sequence, the visual field: 12cm * 12cm, TR/TE:3200ms/102ms, bed thickness/spacing: 3mm/0.3mm, matrix: 256 * 256.
Remove anesthesia: art finish with and the chlorine of anesthetics equal-volume (0.1mL/kg) rather wake up, intramuscular injection is anaesthetized to remove.
Fig. 1 is the crown position of a normal cynomolgus monkey magnetic resonance T1WI image, and Fig. 2 is the crown position of a normal cynomolgus monkey magnetic resonance T2WI image.From this two width of cloth image, can pick out caudatum 1 clearly, shell nuclear 2, dorsal thalamus 3, anterior limb of internal capsule 4, posterior limb of internal capsule 5, island leaf 6, tricorn 7 and corpus callosum 8.
Altogether 24 machins have been carried out brain magnetic resonance surface sweeping according to the method described above, the gained image is all clear effectively.
Claims (2)
1, cynomolgus monkey magnetic resonance scanning method is characterized in that: this method utilizes Philips InteraAchiva 3.0T magnetic resonance machine to scan, and the T1 scanning sequence is IR, the visual field: 120mm, TR/TE:2153ms/13ms, bed thickness/at interval: 3mm/0.3mm, matrix: 256 * 256; The T2 weighting is SE as scanning sequence, the visual field: 12cm * 12cm, TR/TE:3200ms/102ms, bed thickness/spacing: 3mm/0.3mm, matrix: 256 * 256.
2, cynomolgus monkey magnetic resonance scanning method according to claim 1 is characterized in that: before scanning, be that the ketamine of 1:3 and the speed new II mixed liquor of sleeping is anaesthetized machin with volume ratio, the consumption of described mixed liquor is 0.1mL/kg.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008102226399A CN101361659B (en) | 2008-09-19 | 2008-09-19 | Cynomolgus monkey magnetic resonance scanning method |
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| CN2008102226399A CN101361659B (en) | 2008-09-19 | 2008-09-19 | Cynomolgus monkey magnetic resonance scanning method |
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| CN101361659A true CN101361659A (en) | 2009-02-11 |
| CN101361659B CN101361659B (en) | 2011-01-12 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102670203A (en) * | 2012-05-24 | 2012-09-19 | 丰县人民医院 | Rapid scanning method for low field magnetic resonance cerebral general sequence |
| CN103006219A (en) * | 2012-12-25 | 2013-04-03 | 广西玮美生物科技有限公司 | Magnetic resonance scanning method of gastrocnemius muscles of machins |
| CN105193391A (en) * | 2015-10-26 | 2015-12-30 | 成都华西海圻医药科技有限公司 | Detecting method for monkey body neurovirulence test |
-
2008
- 2008-09-19 CN CN2008102226399A patent/CN101361659B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102670203A (en) * | 2012-05-24 | 2012-09-19 | 丰县人民医院 | Rapid scanning method for low field magnetic resonance cerebral general sequence |
| CN102670203B (en) * | 2012-05-24 | 2013-10-30 | 丰县人民医院 | Rapid scanning method for low field magnetic resonance cerebral general sequence |
| CN103006219A (en) * | 2012-12-25 | 2013-04-03 | 广西玮美生物科技有限公司 | Magnetic resonance scanning method of gastrocnemius muscles of machins |
| CN105193391A (en) * | 2015-10-26 | 2015-12-30 | 成都华西海圻医药科技有限公司 | Detecting method for monkey body neurovirulence test |
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| CN101361659B (en) | 2011-01-12 |
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Granted publication date: 20110112 Termination date: 20110919 |