CN109706101A - The thermophilic cold sporosarcina of one kind and its application - Google Patents
The thermophilic cold sporosarcina of one kind and its application Download PDFInfo
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Abstract
The present invention relates to a kind of thermophilic cold sporosarcina and its applications, are thermophilic cold sporosarcina (Sporosarcina psychrophila) KX-1, and deposit number is CGMCC No.16770, and nucleotide sequence is as shown in SEQ ID NO.1.For 3- chlorine carbazole of degrading.The optimum degradation condition of bacterial strain of the present invention are as follows: 3- chlorine carbazole concentration is 50mg/L, inoculum concentration 10%, pH 6.0, temperature is 30 DEG C, and additional 1g/L soluble starch solution is supplementary carbon source, with this condition, after 5d, thermophilic cold sporosarcina reaches 90.0% to the degradation rate of 3- chlorine carbazole.
Description
Technical field
The invention belongs to the microbial technology field for organic pollutant, in particular to a kind of thermophilic cold gemma eight is folded
Coccus and its application.
Background technique
Carbazole (9H-carbazole) and its derivative are a kind of important azaaromatics, are widely used in light
The numerous areas such as electric material, dyestuff, medicine and Supramolecular Recognition.Halogenated carbazole (PHCs) refers to the hydrogen atom on carbazole ring by halogen
A kind of compound replaced plain atom (Cl, Br or I).It is chemically seen in structure, PHCs and polychlorinated dibenzo (PCDFs)
With similar structure.Different according to halogen atom the position of substitution sum number purpose, the PHCs that single halogen replaces is same with 135 kinds
It is object.
Halogenated carbazole is a kind of nitrogen-containing heterocycle aromatic compound, is the important source material of modern daily chemical industry, has extremely
It is widely applied.Halogenated carbazole has teratogenesis, mutagenicity and potential carcinogenicity.Halogenated carbazole structure is stablized, it is difficult to drop
Solution has stronger Environmental Toxicological effect.As a kind of novel lasting organic pollutant, in the environment from the 1980s
It finds for the first time so far, existing more than 20 kinds of halogenated carbazoles are detected in soil and rivers and lakes deposit.But at present about halogen
Research for carbazole environmental behaviour is fewer and fewer, and related data also lacks very much.
Toxicological experiment preliminary studies have shown that, PHCs have persistence, class dioxin toxicity and bioaccumulation.Correlative study
Show that PHCs is similar with PCDFs, the compound of 1,3,6,8 substitution has biggish bio-toxicity.With class dioxin toxicity
That is, PHCs is possible to cause the skin diseases such as chloracne, immunosupress is caused to body, just to endocrine system
Chang Gongneng causes to upset, and influences the sex hormone of bion, leads to the dysplasia of embryo and fetus and leads to cancer.Separately
Outside, soil degrading experiment shows 3- chlorine carbazole and 3, and 6- dibromo carbazole all has no obvious degradation in 450d, shows difficult biology
Degradation characteristic.Cumulative bad and there can be the characteristics such as toxic effect with environmental persistence, biology just because of it to human body, and make
Environment and behavior study for a kind of emerging pollutant, PHCs is relatively fewer at present, therefore, understands environment distribution, the source of PHCs
It is of great significance with eco-toxicology effects to the environmental risk for correctly recognizing this kind of compound.Since most PHCs are not
The chemicals of mankind's synthesis, therefore a possibility that PHCs in environment is from industrial source direct emission, is smaller, and other are related
The production of carbazole derivates uses the important artificial source that then may be PHCs.Such as the production of halogenated bipseudoindoxyl dye is given birth to
At by-product, application as the intermediate of photoelectric material polymer and the herbicide of structure containing parachloroanilinum.In fact, from
There is also some biological enzyme reactions for generating PHCs in right boundary.For example, from fungi (Caldariomyces fumago)
The chloroperoxidase (CPO) of extraction just has this special ability.
Since halogenated carbazole is difficult to be degraded in the natural environment, the persistency organic contaminant for seriously endangering the mankind is formed
Object.The main denitrogenation method industrially used at present is pickling plus the technologies such as hydrogen and absorption, but all cannot efficiently remove these
Nitrogenous compound.Also, compared with other processing methods, microbial degradation organic matter has unrivaled advantage: (1) micro- life
Organic matter can be completely decomposed into carbon dioxide and water by object, for good and all eliminate pollutant, without secondary pollution；(2) degradation process is fast
Speed, expense are low, are the 30%-50% of conventional physical, chemical method expense；(3) degradation process low-carbon energy-saving meets present energy conservation
The environmental protection concept of emission reduction.Therefore, many factors such as comprehensive degradation effect, processing cost, range of applicability consider, microorganism drop
Solution has great superiority.Existing report bromo carbazole such as 3- bromine carbazole can be in Stenotrophomonas category
(Stenotrophomonas sp.) pH 7.0, under the conditions of 30 DEG C of temperature, after 8d, the 3- bromine carbazole that initial concentration is 50mg/L drops
Solution rate only reaches 70% or so.And 3- chlorine carbazole is a kind of relatively conventional chloro carbazole in halogenated carbazole, therefore is easy to sample
Research, has supply in major chemical plant, has the characteristics that application range is wider, therefore, the biology drop of research 3- chlorine carbazole
Solution is of great significance, but existing degradation 3- chlorine carbazole technology yet there are no report in terms of biodegrade.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of thermophilic cold sporosarcina and its applications, have filled up micro- life
The blank of object degradation 3- chlorine carbazole.
The thermophilic cold sporosarcina of one kind of the invention, deposit number are CGMCC No.16770.
The thermophilic cold sporosarcina is thermophilic cold sporosarcina (Sporosarcina psychrophila) KX-
1, nucleotide sequence is as shown in SEQ ID NO.1.
The thermophilic cold sporosarcina, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms
The heart, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, and the deposit date is 2018
November 22.
The bacterium colony of the thermophilic cold sporosarcina is circle, and neat in edge, yellow is smooth, is moistened, protrusion.
The present invention also provides application of the above-mentioned thermophilic cold sporosarcina in degradation 3- chlorine carbazole.
The present invention also provides a kind of methods of 3- chlorine carbazole of degrading, comprising: thermophilic cold sporosarcina is inoculated in 3-
In chlorine carbazole degradation culture medium, cultivated in constant-temperature table.
The present invention also provides the 3- chlorine carbazole degradation culture medium of above-mentioned thermophilic cold sporosarcina, compositions are as follows: (NH4)2SO41.00g、NaCl 1.00g、K2HPO4 1.50g、KH2PO4 0.50g、MgSO4·7H2O 0.20g, 1mL microelement,
1000mL distilled water, 25-200mg/L 3- chlorine carbazole, pH=5.0-9.0；The composition of the microelement are as follows: NaMoO4·2H2O
0.15g、MnSO4·H2O 0.13g、AlCl3·6H2O 0.05g、ZnCl2 0.23g、CuSO4·H2O 0.03g、CoCl2·
6H2O 0.42g, deionized water are settled to 1L, pH=7.0.
The 3- chlorine carbazole degradation culture medium, composition are as follows: (NH4)2SO4 1.00g、NaCl 1.00g、K2HPO4 1.50g、
KH2PO4 0.50g、MgSO4·7H2O 0.20g, 1mL microelement, 1000mL distilled water, 25-200mg/L 3- chlorine carbazole,
1g/L carbon source, pH=5.0-9.0；The composition of the microelement are as follows: NaMoO4·2H2O 0.15g、MnSO4·H2O 0.13g、
AlCl3·6H2O 0.05g、ZnCl2 0.23g、CuSO4·H2O 0.03g、CoCl2·6H2O 0.42g, deionized water are settled to
1L, pH=7.0.
The carbon source is glucose, sucrose, yeast or starch.Preferably starch.
The 3- chlorine carbazole degradation culture medium preferably constitutes are as follows: (NH4)2SO4 1.00g、NaCl 1.00g、K2HPO4
1.50g、KH2PO4 0.50g、MgSO4·7H2O 0.20g, 1mL microelement, 1000mL distilled water, 50mg/L 3- chlorine carbazole,
1g/L starch, pH=6.0；The composition of the microelement are as follows: NaMoO4·2H2O 0.15g、MnSO4·H2O 0.13g、
AlCl3·6H2O 0.05g、ZnCl2 0.23g、CuSO4·H2O 0.03g、CoCl2·6H2O 0.42g, deionized water are settled to
1L, pH=7.0.
The inoculum concentration of the thermophilic cold sporosarcina KX-1 is 10%.
The condition of the culture are as follows: 20-45 DEG C, shaking speed 150rpm, cultivate 120h.
The condition of the culture are as follows: 30 DEG C, shaking speed 150rpm, cultivate 120h.
The present invention still further provides a kind of application of above-mentioned culture medium.
The present invention still further provides a kind of water body purification microorganism formulation, includes above-mentioned thermophilic cold sporosarcina.
Beneficial effect
The optimum degradation item of thermophilic cold sporosarcina (Sporosarcina psychrophila) KX-1 of the invention
Part are as follows: 3- chlorine carbazole concentration is 50mg/L, and inoculum concentration 10%, pH 6.0, temperature is 30 DEG C, and additional 1g/L soluble starch is molten
Liquid is supplementary carbon source, and with this condition, after 5d, thermophilic cold sporosarcina reaches 90.0% to the degradation rate of 3- chlorine carbazole.
Detailed description of the invention
Fig. 1 is the thermophilic cold affiliated Population System chadogram of sporosarcina KX-1 bacterial strain 16SrDNA gene order；
Fig. 2 is the growth curve of thermophilic cold sporosarcina KX-1 bacterial strain；
Fig. 3 is influence of the temperature to thermophilic cold sporosarcina KX-1 degradation 3- chlorine carbazole efficiency；
Fig. 4 is influence of the pH to thermophilic cold sporosarcina KX-1 degradation 3- chlorine carbazole efficiency；
Fig. 5 is influence of the initial concentration to thermophilic cold sporosarcina KX-1 degradation 3- chlorine carbazole efficiency；
Fig. 6 is influence of the additional different carbon source to thermophilic cold sporosarcina KX-1 degradation 3- chlorine carbazole efficiency；
Fig. 7 is to be not added with additional carbon and be added to thermophilic cold sporosarcina KX-1 in the degradation culture medium of additional carbon
To the degradation curve of 3- chlorine carbazole.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, those skilled in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
1, culture medium:
(1) LB culture medium is purchased from raw work bioengineering Shanghai limited liability company.
(2) minimal medium (MSM): (NH4)2SO4 1.00g、NaCl 1.00g、K2HPO4 1.50g、KH2PO4
0.50g、MgSO4·7H2O 0.20g, 1mL microelement, distilled water 1000mL, pH=6.0~8.0.
(3) microelement: NaMoO4·2H2O 0.15g、MnSO4·H2O 0.13g、AlCl3·6H2O 0.05g、ZnCl2
0.23g、CuSO4·H2O 0.03g、CoCl2·6H2O 0.42g adds deionized water to be settled to 1L, pH 7.0.
2, in fluid nutrient medium 3- chlorine carbazole detection: the liquid medium of the 10mL carbazole of chlorine containing 3- is transferred to 250mL's
In separatory funnel, extracted 3 times with 10mL methylene chloride respectively, lower layer's organic phase is incorporated in after anhydrous sodium sulfate absorbs water
In 250mL boiling flask, it is concentrated under reduced pressure into 1mL or so on the rotary evaporator, is then settled to 10mL with chromatography grade acetone, supplies
Efficient liquid phase chromatographic analysis.
1. high performance liquid chromatograph: the silent winged Ultimate 3000 of match
2. mobile phase: acetonitrile: water (80:20, V/V)
3. analytical column: Grace Alltima C18 Column (4.6mm × 250mm, 5 μm)
4. Detection wavelength: 345nm
5. flow velocity 0.5m L/min
6. sample volume: 20 μ L
7. column temperature: 25 DEG C
8. input mode: hand sampling
3,3- chlorine carbazole residual quantity calculation formula is as follows:
Wherein X is the concentration (mg/L) of 3- chlorine carbazole in sample to be tested；AXFor the peak area of 3- chlorine carbazole in sample；A0For
3- chlorine carbazole standard sample peak area；VXFor sample volume (mL)；V0For last constant volume (mL)；C0For 3- chlorine carbazole standard
The concentration (mg/L) of sample.
4, it the preparation of 3- chlorine carbazole stock solution: weighs 0.5g 3- bromine carbazole raw medicine and is dissolved in 100mL chromatography grade acetone, be made into
Concentration is the mother liquor of 5000mg/L, is placed in brown bottle, 4 DEG C of refrigerators save backup.
Embodiment 1
1, strain separating:
(1) activated sludge for acquiring Jiangsu Lan Bo sewage treatment plant, is placed in a long 31.2cm, wide 22.0cm, high
The glass domesticating device of 22.5cm is being not added nutriment, is uninterruptedly being exposed on the basis of water coke slurry of not intaking out with air compressor machine
Gas 3 days.Then it carries out changing water, the mud mixture of about 10L in discharger, nutrient solution is added and is uninterruptedly aerated.
3- chlorine carbazole-acetone soln is initially added into after 10 days while nutrient solution is added having cultivated, 3- chlorine carbazole in holding meanss
Concentration carries out domestication culture in 10mg/L or so, and according to SBR technique (water inlet aeration, stands, drains and leaves unused), changes every other day
Water, culture domestication one month.The preparation of culture solution: 0.6g/L glucose, 0.8g/L anhydrous sodium acetate, 0.3g/L yeast powder,
0.283g/L NH4Cl、0.07g/L K2HPO4·3H2O、0.022g/L KH2PO4.It is configured to about 10 liters or so of nutrient solution.
(2) after precipitating the water sample after domestication 30 minutes, aseptically take supernatant 1mL in being equipped with liquid-transfering gun
In the 250mL sterilizing conical flask of 100mL enriched medium, it is placed on shaking table and cultivates.Shaking speed is 150r/min, temperature 30
℃.The main component of enriched medium EM (Enrichment Medium) are as follows: 10g/L peptone, 5g/L yeast powder, 5g/
LNaCl, distilled water 1000mL, pH=6.8~7.2.
(3) the muddy bacterium solution 1ml of enriched medium is aseptically pipetted in equipped with 100ml domestication culture medium
250ml sterilizes in conical flask, is put into shaking table (conical flask is wrapped up with light-shielding sheet, avoids influence of the illumination to experiment), utilizes 3-
Chlorine carbazole carries out carrying out domestication culture with 5 days for the acclimation method for gradually increasing pollutant concentration of a cycle as carbon source.
Shaking speed is 150r/min, and temperature is 30 DEG C.In minimal medium MSM ((NH4)2SO4 1.00g、NaCl 1.00g、
K2HPO4 1.50g、KH2PO4 0.50g、MgSO4·7H2O 0.20g, 1mL microelement, distilled water 1000mL, pH=6.0~
8.0；Microelement: NaMoO4·2H2O 0.15g、MnSO4·H2O 0.13g、AlCl3·6H2O 0.05g、ZnCl2 0.23g、
CuSO4·H2O 0.03g、CoCl2·6H2O 0.42g adds deionized water to be settled to 1L, pH 7.0.) in be added 3- chlorine carbazole-
Acetone soln prepares the domestication culture medium that 3- chlorine carbazole concentration is 10,20,30,40,50mg/L.
(4) it takes the liquid in the last one period to tame culture medium 1ml, bacterium solution is diluted to 10 by 10 times of dilution methods-1, 10-2, 10-3The bacteria suspension of gradient takes 1ml to be coated on the inorganic salts solid medium of the chlorine carbazole of 3- containing 50mg/L, with sterile painting
The coating of cloth device is uniform.It is placed in 30 DEG C of biochemical cultivation case and cultivates.After growing up to independent clones, the form of each bacterium colony is observed, is used
The bacterium colony of oese picking different shape, separation of crossing in the paved plate of LB solid medium.5-7 is isolated and purified repeatedly
It is secondary, and observe under the microscope, it is ensured that it is to be named as KX-1 after pure single bacterial strain.The single colonie that will be obtained, with 30%
Glycerol saves in -80 DEG C of refrigerators.
2, colony morphology characteristic:
The bacterium colony of thermophilic cold sporosarcina (Sporosarcina psychrophila) KX-1 is round, neat in edge,
Yellow, it is smooth, it moistens, protrusion.
The 16S rDNA gene order of thermophilic cold sporosarcina (Sporosarcina psychrophila) KX-1 is such as
Shown in SEQ ID NO.1；Its affiliated Population System chadogram of 16S rDNA gene order is as shown in Figure 1.
3, the measurement of thalli growth curve:
Bacterial strain KX-1 is inoculated into after expanding culture on LB solid medium, the LB liquid medium after being inoculated in sterilizing
In, in 30 DEG C, constant-temperature table culture under the conditions of 150r/min is sampled every 2h, is measured at 600nm with ultraviolet specrophotometer
Absorbance (OD600), the growth curve for measuring thermophilic cold sporosarcina KX-1 bacterial strain is as shown in Figure 2.
Embodiment 2
The present embodiment chooses temperature, pH, 3- chlorine carbazole concentration, additional carbon, studies the optimum condition of its single factors, sentences
Influence of each factor of breaking to bacterial strain KX-1 degradation 3- chlorine carbazole effect.Each factor is respectively provided with different influence gradients, studies it
In a certain factor influence when, only horizontal to factor setting Different Effects, other factors remain unchanged, behind factor with front
It is carried out premised on each factor optimum condition that experiment obtains.All experiments are respectively provided with three groups of parallel laboratory tests.
The result shows that: the optimum degradation of thermophilic cold sporosarcina (Sporosarcina psychrophila) KX-1
Condition are as follows: 3- chlorine carbazole concentration is 50mg/L, and inoculum concentration 10%, pH 6.0, temperature is 30 DEG C, additional 1g/L soluble starch
Solution is supplementary carbon source, and with this condition, after 5d, thermophilic cold sporosarcina reaches 90.0% to the degradation rate of 3- chlorine carbazole.
It is embodied as follows:
(1) influence of the temperature to 3- chlorine carbazole degradation
100mL minimal medium is added in 250mL conical flask, accesses bacteria suspension to connect bacterium amount 10%, adds
The 3- chlorine carbazole of 50mg/L while being respectively set 3 as degradation solution, while bacterium solution is not added under identical circumstances as control
Group is parallel.It cultivates in 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C and 45 DEG C, pH=6.0,150r/min shaking table, is taken after 5d respectively
Sample detects the residual quantity of 3- chlorine carbazole, is repeated 3 times record average value.To calculate the degradation rate of 3- chlorine carbazole, as a result such as Fig. 3
It is shown, it is known that when temperature is 30 DEG C, degradation effect is best.
(2) influence of the pH to 3- chlorine carbazole degradation
100mL minimal medium is added in 250mL conical flask, accesses bacteria suspension to connect bacterium amount 10%, adds
The 3- chlorine carbazole of 50mg/L while being respectively set 3 as degradation solution, while bacterium solution is not added under identical circumstances as control
Group is parallel.Using hydrochloric acid and sodium hydroxide as the regulator of pH, adjustment pH value is respectively 5.0,6.0,7.0,8.0 and 9.0, simultaneously
Bacterium is not added under identical circumstances as control, while 3 Duplicate Samples are respectively set.In most suitable temperature CMC model, 5d
Sample the residual quantity that 3- chlorine carbazole is detected in HPLC afterwards, to calculate the degradation rate of 3- chlorine carbazole, as a result as shown in figure 4,
It knows as pH=6.0, degradation effect is best.
(3) influence of the 3- chlorine carbazole concentration to degradation
100mL minimal medium is added in 250mL conical flask, accesses bacteria suspension to connect bacterium amount 10%, adds 3-
Chlorine carbazole makes its concentration be respectively 25mg/L, 50mg/L, 100mg/L, 150mg/L and 200mg/L, as degradation solution, simultaneously
3 Duplicate Samples are respectively set.It is cultivated under the conditions of most suitable temperature and pH, the residual quantity of 5d sample detection 3- chlorine carbazole, thus
The degradation rate of 3- chlorine carbazole is calculated, as a result as shown in Figure 5, it is known that when 3- chlorine carbazole concentration is 50mg/L, degradation effect is most
It is good.
(4) influence that additional glucose degrades to 3- chlorine carbazole degradation bacterium
100mL minimal medium is added in 250mL conical flask, accesses bacteria suspension to connect bacterium amount 10%, adds
In addition as degradation solution the glucose of 1g/L is added, in most suitable temperature and pH item in the 3- chlorine carbazole of 50mg/L in conical flask
Under part, be placed in 150r/min shaking table and cultivate, at the same using be only added 3- chlorine carbazole as control, sample detection 3- chlorine click after 5d
The residual quantity of azoles, to calculate the degradation rate of 3- chlorine carbazole, as a result as shown in Figure 6.
(5) influence that additional sucrose degrades to 3- chlorine carbazole degradation bacterium
100mL minimal medium is added in 250mL conical flask, accesses bacteria suspension to connect bacterium amount 10%, adds
In addition as degradation solution the sucrose of 1g/L is added, in most suitable temperature and pH condition in the 3- chlorine carbazole of 50mg/L in conical flask
Under, be placed in 150r/min shaking table and cultivate, while using be only added 3- chlorine carbazole as control, sample detection 3- chlorine carbazole after 5d
Residual quantity, to calculate the degradation rate of 3- chlorine carbazole, as a result as shown in Figure 6.
(6) influence that additional yeast degrades to 3- chlorine carbazole degradation bacterium
100mL minimal medium is added in 250mL conical flask, accesses bacteria suspension to connect bacterium amount 10%, adds
In addition as degradation solution the yeast of 1g/L is added, in most suitable temperature and pH condition in the 3- chlorine carbazole of 50mg/L in conical flask
Under, be placed in 150r/min shaking table and cultivate, while using be only added 3- chlorine carbazole as control, sample detection 3- chlorine carbazole after 5d
Residual quantity, to calculate the degradation rate of 3- chlorine carbazole, as a result as shown in Figure 6.
(7) influence that additional starch degrades to 3- chlorine carbazole degradation bacterium
100mL minimal medium is added in 250mL conical flask, accesses bacteria suspension to connect bacterium amount 10%, adds
In addition as degradation solution the starch solution of 1g/L is added, in most suitable temperature and pH in the 3- chlorine carbazole of 50mg/L in conical flask
Under the conditions of, be placed in 150r/min shaking table and cultivate, while using be only added 3- chlorine carbazole as control, sample detection 3- chlorine after 5d
The residual quantity of carbazole, to calculate the degradation rate of 3- chlorine carbazole, as a result as shown in Figures 6 and 7, it is known that when outer carbon source is starch,
Degradation effect is best, and degradation rate is up to 90% after the 5d that degrades.
SEQUENCE LISTING
<110>Donghua University
<120>a kind of thermophilic cold sporosarcina and its application
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1300
<212> DNA
<213>artificial sequence
<400> 1
tgacgggcgg tgtgtacaag acccgggaac gtattcaccg tggcatgctg atccacgatt 60
actagcgatt ccggcttcat ggaggcgagt tgcagcctcc aatccgaact gggaatgatt 120
ttatgggatt ggctccccct cgcgggttgg caaccctctg tatcatccat tgtagcacgt 180
gtgtagccca ggtcataagg ggcatgatga tttgacgtca tccccacctt cctccggttt 240
atcaccggca gtcaccttag agtgcccaac tgaatgctgg caactaagat caagggttgc 300
gctcgttgcg ggacttaacc caacatctca cgacacgagc tgacgacaac catgcaccac 360
ctgtcaccac tgtccccgaa gggaaaggcg tatctctaca ccggtcagtg ggatgtcaag 420
acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca tgctccaccg cttgtgcggg 480
tccccgtcaa ttcctttgag tttcagcctt gcggccgtac tccccaggcg gagtgcttaa 540
tgcgttagct gcagcactaa ggggcggaaa ccccctaaca cttagcactc atcgtttacg 600
gcgtggacta ccagggtatc taatcctgtt tgctccccac gctttcgcgc ctcagcgtca 660
gttacagacc agaaagccgc cttcgccact ggtgttcctc cacatctcta cgcatttcac 720
cgctacacgt ggaattccgc tttcctcttc tgtactcaag ttctccagtt tccaatgacc 780
ctccacggtt gagccgtggg ctttcacatc agacttaaag aaccgcctgc gcgcgcttta 840
cgcccaataa ttccggacaa cgcttgccac ctacgtatta ccgcggctgc tggcacgtag 900
ttagccgtgg ctttctaata aggtaccgtc atggcacggg cagttactcc cgtacgtgtt 960
cttcccttac aacagagctt tacgatccga aaaccttctt cgctcacgcg gcattgctcc 1020
atcagacttt cgtccattgt ggaagattcc ctactgctgc ctcccgtagg agtctgggcc 1080
gtgtctcagt cccagtgtgg ccgatcaccc tctcaggtcg gctacgcatc gttgccttgg 1140
taggccatta ccccaccaac tagctaatgc gccgcgggcc catcctacag tgacagccga 1200
aaccgtcttt cagagtttgt ccatgcggac aaactgatta ttcggtatta gccccggttt 1260
cccggagtta tccccatctg tagggcaggt tgcccacgtg 1300
Claims (9)
1. a kind of thermophilic cold sporosarcina, deposit number is CGMCC No.16770.
2. thermophilic cold sporosarcina according to claim 1, which is characterized in that the thermophilic cold sporosarcina is thermophilic
Cold sporosarcina (Sporosarcina psychrophila) KX-1, nucleotide sequence is as shown in SEQ ID NO.1.
3. application of the thermophilic cold sporosarcina as described in claim 1 in degradation 3- chlorine carbazole.
4. a kind of method for 3- chlorine carbazole of degrading, comprising: thermophilic cold sporosarcina described in claim 1 is inoculated in 3- chlorine
In carbazole degradation culture medium, cultivated in constant-temperature table.
5. a kind of 3- chlorine carbazole degradation culture medium of thermophilic cold sporosarcina described in claim 1, composition are as follows: (NH4)2SO41.00g、NaCl 1.00g、K2HPO4 1.50g、KH2PO4 0.50g、MgSO4·7H2O 0.20g, 1mL microelement,
1000mL distilled water, 25-200mg/L 3- chlorine carbazole, pH=5.0-9.0；The composition of the microelement are as follows: NaMoO4·
2H2O0.15g、MnSO4·H2O 0.13g、AlCl3·6H2O 0.05g、ZnCl2 0.23g、CuSO4·H2O 0.03g、
CoCl2·6H2O0.42g, deionized water are settled to 1L, pH=7.0.
6. culture medium according to claim 5, composition are as follows: (NH4)2SO4 1.00g、NaCl 1.00g、K2HPO4 1.50g、
KH2PO4 0.50g、MgSO4·7H2O 0.20g, 1mL microelement, 1000mL distilled water, 25-200mg/L 3- chlorine carbazole,
1g/L carbon source, pH=5.0-9.0；The composition of the microelement are as follows: NaMoO4·2H2O 0.15g、MnSO4·H2O 0.13g、
AlCl3·6H2O 0.05g、ZnCl2 0.23g、CuSO4·H2O 0.03g、CoCl2·6H2O 0.42g, deionized water are settled to
1L, pH=7.0.
7. culture medium according to claim 5, composition are as follows: (NH4)2SO4 1.00g、NaCl 1.00g、K2HPO4 1.50g、
KH2PO4 0.50g、MgSO4·7H2O 0.20g, 1mL microelement, 1000mL distilled water, 50mg/L 3- chlorine carbazole, 1g/L form sediment
Powder, pH=6.0；The composition of the microelement are as follows: NaMoO4·2H2O 0.15g、MnSO4·H2O 0.13g、AlCl3·6H2O
0.05g、ZnCl2 0.23g、CuSO4·H2O 0.03g、CoCl2·6H2O 0.42g, deionized water are settled to 1L, pH=7.0.
8. a kind of application of culture medium described in claim 5.
9. a kind of water body purification microorganism formulation includes thermophilic cold sporosarcina described in claim 1.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111534458A (en) * | 2020-04-13 | 2020-08-14 | 浙江工业大学 | Achromobacter TBC-1 and application thereof in degradation of 1,3,6,8-tetrabromocarbazole |
| CN117229961A (en) * | 2023-09-21 | 2023-12-15 | 黑龙江八一农垦大学 | Balanococcus, microbial inoculum and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5616162A (en) * | 1990-04-11 | 1997-04-01 | Idaho Research Foundation, Inc. | Biological system for degrading nitroaromatics in water and soils |
| CN102388064A (en) * | 2009-03-27 | 2012-03-21 | 高裘企业公司 | Compositions and methods for screening and using compounds antagonizing spore-surface interactions |
-
2019
- 2019-02-01 CN CN201910105489.1A patent/CN109706101B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5616162A (en) * | 1990-04-11 | 1997-04-01 | Idaho Research Foundation, Inc. | Biological system for degrading nitroaromatics in water and soils |
| CN102388064A (en) * | 2009-03-27 | 2012-03-21 | 高裘企业公司 | Compositions and methods for screening and using compounds antagonizing spore-surface interactions |
Non-Patent Citations (4)
| Title |
|---|
| YING ZHAO，等: "A psychrophilic leucine dehydrogenase from Sporosarcina psychrophila: Purification, characterization, gene sequencing and crystal structure analysis：web of science，Sporosarcina psychrophile", 《JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC》 * |
| 宋泉颖，等: "球形赖氨酸芽孢杆菌(Lysinibacillus sphaericus)和嗜冷芽孢八", 《微生物学通报》 * |
| 张飞飞，等: "咔唑类化合物研究新进展", 《有 机 化 学》 * |
| 李志伟: "3-溴咔唑降解菌的筛选、鉴定、降解特性及降解途径研究", 《中国硕士学位论文全文数据库工程技术I辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111534458A (en) * | 2020-04-13 | 2020-08-14 | 浙江工业大学 | Achromobacter TBC-1 and application thereof in degradation of 1,3,6,8-tetrabromocarbazole |
| CN111534458B (en) * | 2020-04-13 | 2022-01-14 | 浙江工业大学 | Achromobacter TBC-1 and application thereof in degradation of 1,3,6,8-tetrabromocarbazole |
| CN117229961A (en) * | 2023-09-21 | 2023-12-15 | 黑龙江八一农垦大学 | Balanococcus, microbial inoculum and application thereof |
| CN117229961B (en) * | 2023-09-21 | 2024-03-08 | 黑龙江八一农垦大学 | Balanococcus, microbial inoculum and application thereof |
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