CN103409340B - A kind of microorganism strains and bacterium agent thereof the application in phenolic wastewater is carried out a biological disposal upon - Google Patents
A kind of microorganism strains and bacterium agent thereof the application in phenolic wastewater is carried out a biological disposal upon Download PDFInfo
- Publication number
- CN103409340B CN103409340B CN201310285990.3A CN201310285990A CN103409340B CN 103409340 B CN103409340 B CN 103409340B CN 201310285990 A CN201310285990 A CN 201310285990A CN 103409340 B CN103409340 B CN 103409340B
- Authority
- CN
- China
- Prior art keywords
- phenol
- bacterium
- phe
- bacterial strain
- bacterium agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention discloses a kind of microorganism strains and bacterium agent thereof the application in phenolic wastewater is carried out a biological disposal upon, and described microorganism strains is degraded phenol bacterial strain<i>corynebacterium? lubricantis</i>phe-05, does is deposit number CCTCC? NO:M? 2012031, its bacterium agent can be directly used in containing phenol Industrial Waste Water Treatments, efficiency height, and cost is low, environment can not be caused secondary pollution. Embody rule mode has: (1) provides special efficacy dephenolize bacterium agent for relevant sewage disposal enterprise, turns out novel active mud, extensively processes for all kinds of water body, reduces phenol residual; (2) special efficacy dephenolize bacterium agent is provided for relevant sewage produces enterprise, it is to increase phenol degrading bacterium population quantity in its Sewage treatment systems, it is achieved high-level efficiency, low cost, simple operations, simple management, does not cause the phenolic wastewater biological treatment process of secondary environmental pollution.
Description
Technical field
The present invention relates to the application of efficient phenol degrading bacterium on phenolic wastewater treatment, belong to environmental protection technical field, also belong to the category of biological reinforcing technology.
Background technology
Phenolic wastewater is one of important pollutent of water body. The method of current domestic and international industrial improvement phenolic wastewater is generally divided into physico-chemical process, chemical method, biochemical process three major types. Show according to long-term trial effect, physico-chemical process, chemical method and biochemical process achieve considerable theoretical result and actual effect in the process of industry phenolic wastewater, as: the aldehydes matter in trade effluent is all had removal effect by stripping method, tower extraction process, absorption method, chemical precipitation method, the ordinary activated sludge method of purification, electrolytic cleaning method etc. But, also there are the problems that can not be ignored, often cost is higher in the input disposed of sewage for physico-chemical process, chemical method, and environment can be caused secondary pollution; For biochemical process, the sewage lower containing phenol concentration only can be processed, thus the industrial phenolic wastewater now still unrealized less investment at present to high density, effective process object.
Be directed to above problem, in conjunction with physics, chemistry and biological treatment containing the relative merits of phenol method for industrial waste water, show that there is wide development space in bioremediation through decades of research. The biological pathway of current own basic understanding microbiological deterioration phenol mainly contains two: 1. aerobic microbiological utilizes phenol hydroxylase to be pyrocatechol by oxidation of phenol, acetyl-CoA, succsinic acid, pyruvic acid etc. can enter tricarboxylic acid cycle, continue to be utilized by microorganism; 2. it is 4-HBA by anaerobion by phenol carboxylation, then through the effect phenylformic acid approach of relevant enzyme, generates acetyl-CoA, finally change into acetic acid, continue to be utilized by microorganism. From this path analysis it may be seen that adopt the phenol microbiological deterioration sewage can carry out under anaerobism or aerobic conditions, therefore, microorganism falls phenol and possesses wider Technological adaptability from principle.
However, set up an efficient microorganism phenolic wastewater treatment technique, still needing to carry out a large amount of previous works, how by the naturalized strain being separated, screening acquired character is excellent, and the microbiobacterial agent developing adaptation production technique further is crucial. And at present, the falling phenol bacterium and can tolerate the concentration of phenol mostly lower (< 1500mg/L) of great majority report; Falling phenol rate and be generally no more than 90%, the research report for high-concentration phenolic wastewater microorganism is then few.
Summary of the invention
The present invention is intended to screen, tame and develop bacterial classification, the bacterium agent of microorganism AgromycessoliPhe-03 and the CorynebacteriumlubricantisPhe-05 of aldehydes matter in relevant efficient degradation industrial sewage, and the process for industry phenolic wastewater, to realize the high-level efficiency processed, low cost, pollutes little effect.
The present invention is achieved through the following technical solutions above-mentioned technological invention object.
(1) chemical environment waste water and neighbouring soil extract microbial bacterial group is utilized, by only phenol being cultivation on the substratum of carbon source, then through obtaining bacterial strain Phe-03 and Phe-05 after screening for several times and domestication in high-concentration industrial phenol-containing wastewater. Through molecular biology identification, Phe-03 is accredited as Agromycessoli bacterial strain; Phe-05 is accredited as Corynebacteriumlubricantis bacterial strain. Two bacterial strains have carried out effective preservation on February 22nd, 2012 in " China typical culture collection center " all, and (China typical culture collection center is called for short CCTCC, also Wuhan University's preservation center is cried, address is: life science institute of Wuhan University of Wuhan City, postcode: 430072). Deposit number is respectively: AgromycessoliPhe-03CCTCCNO:M2012030, CorynebacteriumlubricantisPhe-05CCTCCNO:M2012031; By the content of Artificial Control phenol, for the experimental model of bacterium AgromycessoliPhe-03 and CorynebacteriumlubricantisPhe-05 degradation of phenol ability, efficiency and related detecting method Erecting and improving.
(2) by adopting the domestication of actual industrial phenolic wastewater further, obtain the mixed bacterium agent of suitable sewage environment, and process for pilot scale. Taking common Sewage treatment systems result as blank, compare the effect of bacterium agent on industry phenolic wastewater treatment.
Embodiment
The strain improvement of example 1AgromycessoliPhe-03, CorynebacteriumlubricantisPhe-05 and bacterial preparation process
(1) minimal medium preparation:
By 0.5gKH2PO4��0.5gK2HPO4��0.2gMgSO4��0.2gCaCL2, 0.2gNaCl, micro Fe SO4It is placed in beaker with water to mix, and adjusts between its pH to 7.4-7.6 with NaoH, stir evenly i.e. obtained minimal medium.
(2) preliminary screening of phenol bacterium:
Liquid for 1000ml minimal medium is dispensed into (without the need to sterilizing) in the triangular flask of 5 500ml with 200ml/ bottle, and is numbered: P5��P10��P15��P20��P25, and to P5��P10��P15��P20��P25The middle phenol adding 0.5g/l, 1.0g/l, 1.5g/l, 2.0g/l, 2.5g/l respectively, subsequently to the mud sample adding 5ml in each triangular flask, is positioned over 28 DEG C, cultivates 48h in the shaking table of 210rmp, then respectively from P5��P10��P15��P20��P25Respectively getting a sample is applied on slide, dyes with methylene blue after drying, and observes bacterial growth situation in each triangular flask under the microscope. Observations shows: incrementally observe with phenol concentration, P10��P15Bacterial growth quantity is more, and is evenly distributed; And P25Upper substantially without bacterial growth.
(3) separation and purification of phenolic wastewater bacterium
The inorganic salt nutrient solution prepared and culture dish it is placed at high-pressure sterilizing pot 121 DEG C sterilizing 30min, takes out nutrient solution and plate, respectively to P10��P15��P20��P25Add the phenol solution of 1ml, 1.5ml, 2ml, 2.5ml50%, and mix even with nutrient solution, be down flat ware with 30ml/ ware, take out P10��P15��P20��P25On bacterial strain carry out separation and purification. With 200ml sterilized water respectively by P10��P15��P20��P25Bacterium liquid is diluted to 10-3��10-4��10-5Three concentration gradients, and coat plate according to each concentration gradient 4 ware, amount to 4 �� 3 �� 4=48 plate, 28 DEG C, 48h cultivated by 210rmp temperature case. Show through observations:
1. bacterial strain kind, growing way, quantity, color, shape are all different along with the difference of phenol concentration and extension rate, and along with the rising of phenol concentration, bacterial strain kind is more single, and quantity is more few; Also along with extension rate rising and bacterial strain quantity is more few.
2. by isolation medium plate observed and recorded, by the difference of phenol concentration and extension rate, selected 38 strain bacterium colonies carry out next step separation and purification, it is respectively phenol concentration is 30 strains cultivated under 1.0mg/ml, phenol concentration is 3 strains cultivated under 1.5mg/ml, phenol concentration is 4 strains cultivated under 2.0mg/ml, and phenol concentration is 1 strain cultivated under 2.5mg/ml.
(4) the further optimal screening of phenolic wastewater bacterium
Method, equipment, reagent and step are identical with " separation and purification of phenol degrading bacterium ", the 38 strain bacterium that " separation and purification of phenol degrading bacterium " is selected afterwards adopt higher phenol concentration substratum carry out adaptability domestication and cultivates. Through observing, result shows:
1. phenol concentration is when 2000mg/ml, P10Concentration gradient is 10-3With 10-2Bacterial strain growing way good compared with other concentration gradients, and bacterial classification is identical, and apparent is orange one-tenth sheet, and Uniform Name is phe-01;
2. phenol concentration is when 2000mg/ml, p15Concentration gradient is 10-3Bacterial strain growing way good compared with other concentration gradients, apparent is orange one-tenth sheet, called after phe-02;
3. phenol concentration is when 2500mg/ml, p20Concentration gradient is 10-2Bacterial strain growing way good compared with other concentration gradients, apparent become sheet for white light yellow complexion, called after AgromycessoliPhe-03;
4. phenol concentration is when 2000mg/ml, P20Concentration gradient is 10-2With 10-4Bacterial strain growing way good compared with other concentration gradients, and bacterial classification is identical, apparent becomes sheet for white particle, and Uniform Name is phe-04;
5. phenol concentration is when 2000mg/ml, P20Concentration gradient is 10-2Have a bacterial strain with 4. in bacterium for of the same race, and growing way is good compared with other concentration gradients, apparent becomes sheet called after CorynebacteriumlubricantisPhe-05 for white light yellow complexion.
(5) phenolic wastewater bacterium phenol degrading quantitative screening
1. phenol Standard curve is set up
The volumetric flask that 5.00g phenol is placed in 500ml is weighed with electronics sky chessboard, add the fixed molten 500ml that arrives of pure water dilution as mother liquor, and then accurately draw in the volumetric flask that mother liquor 0.5ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml, 3.0ml, 3.5ml, 4.0ml, 4.5ml, 5.0ml join 10 50ml cleanings respectively with moving liquid rifle, and it is dissolved to 50ml with pure water, it is numbered 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, 500mg/ml, 600mg/ml, 700mg/ml, 800mg/ml, 900mg/ml, 1000mg/ml; Surely solvent phenol solution is placed in after refrigerator refrigerates 24h and measures with high performance liquid chromatograph, do phenol concentration and the typical curve of peak area relation. The HPLC solvent systems of high performance liquid chromatography is second eyeball: pure water=8:2; Chromatographic column model is Japan YMC phenol dedicated analysis post (hydrosphereC18. 250 �� 4.6mmI.D.S-5um, 12nm), post temperature is 37 DEG C. Result draws typical curve equation: y=4382.7X+24055, R2=0.9969; " y " is phenol HPLC mensuration peak area, and " X " is phenol concentration.
2. phenol screening quantitatively falls in phe-01 ~ phe-05 shake-flask culture
Minimal medium compound method is the same. Add phenol mother liquor after medium sterilization, make phenol concentration in culture system reach 1500mg/ml. Aseptically, from the inclined-plane kept, provoke phe-01 ~ phe-05 bacterium bacterium tongue respectively in shaking flask with inoculating needle, it is placed in 28.5 DEG C, cultivate when 200rpm. In the process, (phenol concentration is 1500mg/ml to arrange phenol nutrient solution blank; Not inoculated bacteria). Starting sampling after cultivating 8h, sample 100ul with standard sampling container every time, then use 100ul distilled water diluting, phenol concentration in HPLC quantitative assay culture system, measuring method is the same, and calculates phenol degrading rate. Sampling time interval 8h, i.e. 8h, 16h, 24h, 32h, 40h and 48h sampling. Sampling determination result shows: in 32h, phe-03 falls phenol rate and reaches 81.08%, phe-05 and fall phenol rate and reach 83.02%; Phe-01, phe-02, phe-04 fall phenol rate and are respectively 37.06%, 36.51% and 48.13%; The phenol nature evaporation rate of blank is only 1.28%. By above work, it is determined that phe-03 and phe-05 is best selected bacterial strain.
(6) selected bacterial strain electron-microscope scanning is observed
In every centrifuge tube, pipette 5ml glutaraldehyde with transfer pipet, and it is numbered phe-03 and phe-05. Choose under spirit lamp conditions for sterilization between ultraviolet is inoculated and get appropriate bacterium tongue and be placed in the centrifuge tube that 2 are equipped with glutaraldehyde, and mixed even, build lid and seal up for safekeeping, after spending the night, pipette appropriate bacterium liquid on slide with transfer pipet, after drying, the process of spray gold, then uses electron microscope observation. Observations shows: two strain bacterium are spherical bacterium. The about 0.5um of thalline diameter, length 0.5-1.0um are not etc.
(7) phe-03, phe-05 identification of strains
Bacterial strain phe-03 and phe-05 passes through total DNA extraction, the general primer amplification of bacterial 16 S rDNA. Amplification object fragment is by utilizing Blast software to carry out similarity searching in GenBank database at GenBank etc. after order-checking, and the 16SrDNA sequence of the typical strain selecting tetraploid rice high than object, then uses CLUSTALX software as ginseng]Carry out multisequencing comparison and calculate examination bacterial strain and join than the sequence similarity between bacterial strain, adopt adjacent method (Neighbor-joining) MAGE4 software building supplying examination bacterium and joining than the phylogenetic tree between bacterium based on 16SrDNA sequence, analytical results shows: phe-03 belongs to Agromycessoli bacterial strain, and phe-05 is that Corynebacteriumlubricantis belongs to bacterial strain.
Two strain bacterium have carried out effective preservation on February 22nd, 2012 in " China typical culture collection center " all. Deposit number is respectively: AgromycessoliPhe-03CCTCCNO:M2012030, CorynebacteriumlubricantisPhe-05CCTCCNO:M2012031.
The single bacterial strain of example 2phe-03, phe-05 is used on industry high-concentration phenolic wastewater
(1) coal chemical industrial waste water water quality measures
First adopt water sample from coal chemical industrial waste water source respectively and amount to 50L, measure the indexs such as the phenol content in coal chemical industry sewage, CODcr, ammonia nitrogen, sulfide after mixing respectively through the method such as high performance liquid chromatograph, potassium dichromate process, result is shown as: C(phenol)=750.0mg/L, CODcr=5035.10mg/L, C (ammonia nitrogen)=800.0mg/L, C(sulfide)=40.0mg/L, ph=9.5, SS=650.0mg/L, oils=200mg/L.
(2) the single bacterial strain domestication of phe-03, phe-05
Coal chemical industrial waste water 2000ml, after adopting sterilised membrane filter to filter, is distributed into 20 bottles (100ml/ bottles). Every 10 bottles are accessed phe-03 and phe-05 strain cultured solution (nutrient solution cultivates 48h in advance, cultivates salt+1000mg/ml phenol based on composition) respectively in 10% ratio, and are placed in shaking table, 28.5 DEG C, cultivate when 200rpm. Timing sampling and observation. Sampling is started, sampling time interval 24h, i.e. 24h, 48h, 72h, 96h, 108h and 132h sampling after cultivating 24h. Observe and sampling determination result shows: phe-03 bacterial strain growing way after 96h is fine, occurs a large amount of flocks bottom shaking flask, and bacterium liquid is muddy, fall phenol rate in 108h ~ 132h and reach and be increased to rapidly 87.15% from 32.34%. Phe-05 bacterial strain growing way after 96h is fine, and bacterium liquid is muddy, falls phenol rate and reach and be increased to rapidly 90.05% from 24.51% in 96h ~ 132h.
(3) the single bacterial strain of phe-03, phe-05 is tamed further
Coal chemical industrial waste water 1000ml, after adopting sterilised membrane filter to filter, is distributed into 10 bottles (100ml/ bottles). Every 5 bottles are accessed phe-03 and phe-05 bacterial strain 96h domestication liquid (acclimation process is the same) respectively in 10% ratio, add inorganic salt in proportion, be placed in shaking table, 28.5 DEG C, cultivate when 200rpm. Timing sampling and observation. Sampling is started, sampling time interval 24h, i.e. 24h, 48h, 72h, 96h, 108h and 132h sampling after cultivating 24h. Observation and sampling determination result show: phe-03 bacterial strain continues domestication through 7 generations, and growing way is fine after 24h, and bacterium liquid is muddy, falls phenol rate and reach 86.15% in 48h. Phe-05 bacterial strain continues domestication through 11 generations, and growing way is fine after 24h, and bacterium liquid is muddy, falls phenol rate and reach 80.78% in 48h.
(4) the single bacterial strain active bacteria agent of phe-03, phe-05 is cultivated
By the pattern amplified step by step, phe-03, phe-05 tame bacterium liquid and are amplified in the open aerated culture tank of 200l from 1000ml cultivation amount. Culture tank adopts hot water circulation heated, and general gas blower ventilates, and general-purpose machine stirs. 28 DEG C, 120rpm cultivation, air flow is 50L/min. Incubation time 48h.
Example 3phe-03, phe-05 hybrid bacterial strain is applied on industry high-concentration phenolic wastewater treatment
(1) Sewage treatment systems is set up
Produce enterprise's production area Sewage treatment systems technical process according to phenolic wastewater and build a set of day process 5m3Pilot scale Sewage treatment systems model, that is: production waste is through grid, enters coagulation basin, then to MESB (acid adding), then enters efficient sedimentation tank, flows into hydrolysis acidification pool, then to MBBR pond, PAC pond, then flows into oxidative decoloration pond, last water outlet.
(2) phenolic wastewater produces enterprise's phenolic wastewater water quality mensuration
Selecting the indexs such as phenol content that coal chemical industrial waste water source measures in coal chemical industry sewage through the method such as high performance liquid chromatograph, potassium dichromate process respectively, CODcr, ammonia nitrogen, sulfide, result is shown as: C(phenol)=810.0mg/L, CODcr=5416.05mg/L, C (ammonia nitrogen)=810.21mg/L, C(sulfide)=51mg/L, ph=9.13, SS=712.0mg/L, oils=152mg/L.
(3) practical application of phe-03, phe-05 mixt bacteria
Phe-03, phe-05 are mixed the MBBR pond that thin agent (each 100L) joins in pilot scale Sewage treatment systems by every 168h, and after the bacterium adaptive process in early stage of 30 days, microorganism is placed in Sewage treatment systems surely.
After microorganism has been put surely, carry out mix bacterium agent degradation of phenol test. Every 8h samples once. Sampling time interval 8h, i.e. 8h, 16h, 24h, 32h, 40h and 48h sampling. The effluent quality producing enterprise practical sink drainage with phenolic wastewater contrasts. Result shows: the degraded containing the aldehydes matter in phenol processing wastewater is reached expected effects by phe-03, phe-05 mixt bacteria in pilot scale model, containing phenol concentration in the wastewater treatment effluent quality of pilot scale model 24h is 0.05mg/l, phenol clearance reaches more than 95%, than comparison raising 23%. Sink drainage water quality reaches one-level sewage drainage standard from three grades of discharges.
Claims (2)
1. a deposit number is the degraded phenol bacterial strain CorynebacteriumlubricantisPhe-05 of CCTCCNO:M2012031, it is characterized in that: the microbial bacterial group utilizing chemical environment waste water and neighbouring soil sample, by taking phenol as the substratum screening of sole carbon source, adopting high density to contain the domestication of phenol industrial sewage again, bacterial strain Pyrogentisinic Acid has excellent degradation property; This bacterial strain tolerance phenol concentration reaches 2000mg/L, and bacterium colony is apparent becomes sheet for white light yellow complexion.
2. deposit number be CCTCCNO:M2012031 degraded phenol bacterial strain CorynebacteriumlubricantisPhe-05 wastewater containing phenol process on an application, it is characterized in that: the bacterium agent of CorynebacteriumlubricantisPhe-05 be used for wastewater containing phenol biological treatment.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210057537.2A CN102776137B (en) | 2012-03-07 | 2012-03-07 | Application of microorganism bacterium agent in biological treatment of phenolic wastewater |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210057537.2A Division CN102776137B (en) | 2012-03-07 | 2012-03-07 | Application of microorganism bacterium agent in biological treatment of phenolic wastewater |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103409340A CN103409340A (en) | 2013-11-27 |
| CN103409340B true CN103409340B (en) | 2016-06-01 |
Family
ID=47121247
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210057537.2A Active CN102776137B (en) | 2012-03-07 | 2012-03-07 | Application of microorganism bacterium agent in biological treatment of phenolic wastewater |
| CN201310285990.3A Active CN103409340B (en) | 2012-03-07 | 2012-03-07 | A kind of microorganism strains and bacterium agent thereof the application in phenolic wastewater is carried out a biological disposal upon |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210057537.2A Active CN102776137B (en) | 2012-03-07 | 2012-03-07 | Application of microorganism bacterium agent in biological treatment of phenolic wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN102776137B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104845890B (en) * | 2015-02-09 | 2017-10-31 | 暨南大学 | Applications of earth mould (Agromyces sp.) the MT E in a variety of phthalic acid esters of degrading |
| CN104845891B (en) * | 2015-02-09 | 2017-11-24 | 暨南大学 | A kind of earth mould bacteria suspension of a variety of phthalic acid esters of degrading and its application |
| CN105712498B (en) * | 2016-04-28 | 2018-05-08 | 河海大学 | A kind of biological treatment device of phenol wastewater |
| CN106566768A (en) * | 2016-11-04 | 2017-04-19 | 上海昭泉环境技术有限公司 | Combined type bio-membrane reactor combined with mycelium pellet and method for phenolic waste water treatment by bio-membrane reactor |
| CN109097435A (en) * | 2018-09-01 | 2018-12-28 | 长沙理工大学 | A method of screening malaga carbohydrate oxidase strain from air |
| CN111471630B (en) * | 2020-06-11 | 2021-11-02 | 鲁东大学 | Corynebacterium Ytld-phe09 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2061752C1 (en) * | 1993-04-26 | 1996-06-10 | Саратовский филиал Государственного научно-исследовательского института генетики и селекции промышленных микроорганизмов | Strain of bacterium corynebacterium species - a destructor of aromatic compounds |
| EP0694611B1 (en) * | 1994-05-30 | 2000-02-02 | Canon Kabushiki Kaisha | Corynebacterium sp. J1, method for biodegradation of aromatic compounds and/or chlorinated organic compounds, and method for environmental remediation using it |
| CN101805713A (en) * | 2010-02-05 | 2010-08-18 | 北京大学 | Corynebacterium sp strain and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100429304C (en) * | 2006-12-05 | 2008-10-29 | 中国科学院武汉病毒研究所 | Bacillus alcaligenes strain for degrading ortho-nitrophenol and preparing method |
-
2012
- 2012-03-07 CN CN201210057537.2A patent/CN102776137B/en active Active
- 2012-03-07 CN CN201310285990.3A patent/CN103409340B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2061752C1 (en) * | 1993-04-26 | 1996-06-10 | Саратовский филиал Государственного научно-исследовательского института генетики и селекции промышленных микроорганизмов | Strain of bacterium corynebacterium species - a destructor of aromatic compounds |
| EP0694611B1 (en) * | 1994-05-30 | 2000-02-02 | Canon Kabushiki Kaisha | Corynebacterium sp. J1, method for biodegradation of aromatic compounds and/or chlorinated organic compounds, and method for environmental remediation using it |
| CN101805713A (en) * | 2010-02-05 | 2010-08-18 | 北京大学 | Corynebacterium sp strain and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| Biodegradation of phenol using Corynebacterium sp.DJ1 aerobic granules;Kuo-Ling Ho et.al;《Bioresource Technology》;20090621;第100卷;第5051-5055页，尤其是摘要及第3节 * |
| 高效降酚菌株JY03的筛选及其降解特性研究;姜立春等;《环境工程学报》;20110831;第5卷(第6期);第1912-1916页，尤其是摘要及第1.2节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102776137A (en) | 2012-11-14 |
| CN102776137B (en) | 2014-06-18 |
| CN103409340A (en) | 2013-11-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhou et al. | Simultaneous removal of inorganic and organic compounds in wastewater by freshwater green microalgae | |
| CN111423996B (en) | Complex microbial inoculant, preparation method thereof and application thereof in soil remediation field | |
| CN103409340B (en) | A kind of microorganism strains and bacterium agent thereof the application in phenolic wastewater is carried out a biological disposal upon | |
| CN108611285B (en) | Sulfonamide antibiotic degrading bacteria and application thereof | |
| CN106434470B (en) | A kind of polycyclic aromatic hydrocarbon-degrading bacteria and its application | |
| CN114890555B (en) | Solid microbial preparation for treating rural black and odorous water body and preparation method and application thereof | |
| CN105713862B (en) | The bacterial strain and its application of degradable pyridine and ammonia nitrogen | |
| Dalvi et al. | Removal of pollutants from wastewater via biological methods and shifts in microbial community profile during treatment process | |
| CN113215033B (en) | Sulfonamide antibiotic degrading bacteria and application thereof | |
| CN104263682A (en) | Plant-growth-promoting endophytic bacterium having polycyclic aromatic hydrocarbons degrading function and application thereof | |
| CN109337825B (en) | Paecilomyces beijing strain LYZ7 and application thereof | |
| CN107446842A (en) | One bacillus subtilis and its application in purifying water | |
| CN110229766A (en) | Aoxidize microbacterium and its application in degradable organic pollutant | |
| CN106434413A (en) | Raoultella planticola and method for degrading pyrene in soil with the same | |
| Wang et al. | Simultaneous nutrient and carbon removal from azo dye wastewater using a photorotating biological contactor reactor | |
| CN105349481B (en) | A kind of screening technique of Chinese yew rhizosphere polycyclic aromatic hydrocarbon-degrading bacteria and application | |
| CN105199988B (en) | One plant of root telogenesis film bacterium RS2 with luxuriant and rich with fragrance degradation function and its application | |
| CN116254188B (en) | Strain SRB-6 and application thereof in degrading herbicide acetochlor under anaerobic condition | |
| CN110511975A (en) | A kind of brominated waste-water treatment efficient bacterium screening and culturing experimental method | |
| CN113373066B (en) | Humic acid degrading fungus HA-Z3 and application thereof | |
| CN101781624B (en) | Bacterial strain for treating oilfield produced water | |
| CN110261267B (en) | Detection method of photosynthetic bacteria preparation product for fishing | |
| Rajani et al. | Role of purple nonsulfur bacteria Rhodopseudomonas palustris RSOU000 and Rhodopseudomonas thermotolerance RSOU555 in waste water treatment | |
| CN110669716A (en) | Method for separating high-concentration phenol and heavy metal resistant and low-temperature resistant rhodococcus | |
| CN114437954B (en) | Bacillus curvatus for degrading sulfonated drilling fluid, mixed bacteria and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |