CN109680345A - The biological extraction process of fiber in a kind of waste agronomic crop bast raw material - Google Patents
The biological extraction process of fiber in a kind of waste agronomic crop bast raw material Download PDFInfo
- Publication number
- CN109680345A CN109680345A CN201910136851.1A CN201910136851A CN109680345A CN 109680345 A CN109680345 A CN 109680345A CN 201910136851 A CN201910136851 A CN 201910136851A CN 109680345 A CN109680345 A CN 109680345A
- Authority
- CN
- China
- Prior art keywords
- raw material
- dce
- extraction process
- biological extraction
- fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a kind of biological extraction process of fiber in waste agronomic crop bast raw material, including, immersion treatment is carried out to waste agronomic crop bast raw material with water, filtrate and fibrous raw material are collected after separation of solid and liquid respectively, DCE-01 bacteria suspension is made in the maturing fermentation liquid that DCE-01 bacterium is then added into filtrate, and fibrous raw material degumming is impregnated with DCE-01 bacteria suspension, it is then refined with Laccase/Mediator, is dehydrated after cleaning to obtain the final product.The present invention is refined by the degumming of DCE-01 bacterium and Laccase/Mediator, can moderately remove the colloids such as the pectin, hemicellulose, lignin of banana stem and arghan raw material；The present invention is obtained pineapple leaves and banana bast fiber, was both turned waste into wealth by the green processing mode of " efficiently, energy-saving and environmental protection ", it increases economic efficiency, the source of weaving natural fiber has also been expanded simultaneously, and the economic benefit of comprehensive utilization is good, has a extensive future.
Description
Technical field
The present invention relates to the application technology as the second resource fields of waste agronomic crop, and in particular to a kind of waste agronomic crop bast is former
The biological extraction process of fiber in material.
Background technique
China is one of World banana and pineapple main production country, has the banana of up to ten million tons and pineapple edible for people every year,
Also there is the almost generation such as the banana of equivalent and pineapple by-product such as banana stem, pineapple leaves simultaneously, these byproducts overwhelming majority is
It is abandoned when rubbish, seriously pollutes environment.Contain a large amount of cellulose fibres in banana stem and pineapple leaf, if opening it
Hair utilizes, and can not only expand the source of weaving natural fiber, moreover it is possible to protect environment.
The chemical composition of banana bast fiber and arghan is similar with other bast-fibres, shown in table 1 specific as follows,
It contains more glue impurity, wherein it is especially higher with content of lignin, it is much higher than ramie and flax, and it is lower than jute, it is therefore, fragrant
The pliability of any of several broadleaf plants bast fiber and arghan, spinning property are inferior to ramie, flax better than jute.
1 arghan of table, banana bast fiber, flax and tossa chemical component/%
In order to improve fiber spinnability, gum level in fiber is reduced, spinning yarn quality, banana bast fiber and pineapple are improved
Leaf fibre should take degumming process appropriate before spinning.The single fiber length of pina fibre is shorter, it is necessary to pass through half deguming
Guarantee with the presence of certain residue glue, by very short single fiber adhesion at the bundle fiber for meeting spinning process requirement.
The method of traditional extraction banana and pina fibre mainly has artificial process, Mechanical Method and three kinds of chemical method.Manually mention
The problems such as to follow the example of that there are labor costs high, fiber stiffness is big, Mechanical Method are saved artificial, but fibre strength is lower, and damage is big,
It is unable to satisfy the requirement of spinning.Chemical reaction flows fiber, modified pina fibre is after deep processing is handled, white appearance,
It is soft smooth, excellent strength, but environmental pollution is serious.Therefore, the biological extraction process ten of banana and pina fibre is developed
Divide necessity.
The basis of biological extraction process is to obtain to remove multiple functional degumming enzyme system for banana and pina fibre colloid,
Including pectase, zytase, mannonase laccase etc., but to obtain and meanwhile secrete these enzymes strain it is very difficult.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of biologies of fiber in waste agronomic crop bast raw material
Extracting method.
In order to achieve the above objectives, the invention adopts the following technical scheme:
The biological extraction process of fiber in a kind of waste agronomic crop bast raw material, including, with water to waste agronomic crop bast
Raw material carries out immersion treatment, collects filtrate and fibrous raw material respectively after separation of solid and liquid, and DCE-01 bacterium is then added into filtrate
DCE-01 bacteria suspension is made in maturing fermentation liquid, and impregnates fibrous raw material degumming with DCE-01 bacteria suspension, then with Laccase/Mediator essence
Refining is dehydrated after cleaning to obtain the final product.
In the above-mentioned technical solutions, the immersion Degumming method of the DCE-01 bacteria suspension are as follows: 33-35 DEG C, 120-150r/
Min degumming 10-15h.
Further, in the above-mentioned technical solutions, in the DCE-01 bacteria suspension, the concentration of the DCE-01 bacterium is greater than 1
×108cfu/mL。
Still further, in the above-mentioned technical solutions, the maturing fermentation liquid of the DCE-01 bacterium the preparation method comprises the following steps: will
DCE-01 strain is seeded in GR culture solution and mixes, and 5-6h is cultivated at 33-35 DEG C, and it is flat that YQ nutrient agar is coated on after dilution
In plate, single colonie is separated after cultivating 18-20h at 33-35 DEG C, colonies typical is selected and is seeded in GR culture solution and mixes,
Culture 5-6h obtains primary seed solution under the conditions of 33-35 DEG C and 150-180r/min, and primary seed solution is then seeded to 6-10 times
The GR culture solution of volume, culture 5-6h obtains secondary seed solution under the conditions of 33-35 DEG C and 150-180r/min, finally by second level kind
Sub- liquid is seeded to the FJ culture solution of 20-50 times of volume, cultivates 8-10h under the conditions of 33-35 DEG C and 150-180r/min to obtain the final product.
Further, in the above-mentioned technical solutions, the mass ratio formula of the GR culture solution are as follows: glucose 1%+ beef
Cream 0.5%+ peptone 0.5%+NaCl 0.5%, and be 7.0-7.5 with NaOH tune pH value.
Further, in the above-mentioned technical solutions, the mass ratio formula of the YQ nutrient agar panel are as follows: glucose
0.5%+ nutrient agar 3.5%.
Further, in the above-mentioned technical solutions, the mass ratio formula of the FJ culture solution are as follows: glucose 2%+ corn
Paste dry powder 2%+ bean cake powder 1%+ peptone 0.01%+NaNO30.15%.
In the above-mentioned technical solutions, the method for the Laccase/Mediator refining are as follows: by the laccase HAc- of pH value 4-5.5
NH4It is 500U/mL that Ac buffer, which is diluted to concentration, and 2,2- of addition joins nitrogen-two (3- ethyl-benzothiazole -6- sulfonic acid) di-ammonium salts
To concentration 0.2-0.5wt%, the fibrous raw material after degumming is impregnated by bath raio 1: 12-18, reacts 2- at 38-45 DEG C after mixing
4h。
Further, in the above-mentioned technical solutions, the immersion treatment is ultrasonic pretreatment after water impregnates, the immersion
The bath raio of processing is 1: 15-20, and the processing time is 15-30min.
Still further, in the above-mentioned technical solutions, the frequency and power of the ultrasonic wave be respectively 80-100KHz and
200-300W。
Further, in the above-mentioned technical solutions, the biological extraction process further includes place after cleaning and being dewatered
Reason, the post-processing successively include oil supply deoiling treatment and drying process.
Preferably, in the above-mentioned technical solutions, the method for the deoiling treatment are as follows: will clean and dewatered bast fiber
It shakes up to be placed in the BSK solution that concentration is 1-2wt% and impregnates 10-20min, the drying temperature of the drying process is lower than 50
℃。
Also further, in the above-mentioned technical solutions, the waste agronomic crop bast raw material be pineapple leaves bast raw material or
Banana stem raw material.
Advantages of the present invention:
(1) in a kind of waste agronomic crop bast raw material provided by the present invention fiber biological extraction process, by using energy
The degumming high-efficiency strain DCE-01 bacterium degumming of broad spectrum activity crudefiber crop and the paint of pectase, mannase and zytase are secreted simultaneously
Enzyme/mediator refining, can moderately remove the colloids such as the pectin, hemicellulose, lignin of banana stem and arghan raw material, gram
The strain problem for being difficult to acquire energy while a variety of degummases of efficient secretion is taken；
(2) in a kind of waste agronomic crop bast raw material provided by the present invention fiber biological extraction process, by " efficiently,
The green processing mode of energy-saving and environmental protection ", obtains pineapple leaves and banana bast fiber, and tropical agriculture by-product is both rationally utilized
Object pineapple leaves and banana stem, turn waste into wealth, and realize fruit, leaf, stem dual-purpose, improve the economic benefit of banana and pineapple cultivation industry,
The source of weaving natural fiber has also been expanded simultaneously, and the economic benefit of comprehensive utilization is good, has a extensive future.
Detailed description of the invention
Fig. 1 is the technique of the biological extraction process of fiber in waste agronomic crop bast raw material provided by the embodiment of the present invention
Flow chart.
Specific embodiment
In the following with reference to the drawings and specific embodiments, specific embodiments of the present invention will be described in further detail.
The following examples are intended to illustrate the invention, but is not limited to protection scope of the present invention, protection model of the invention
It encloses and is subject to claims.
Unless otherwise specified, experiment reagent used in the embodiment of the present invention and material etc. are commercially available, if not having
Body indicates, the conventional means that technological means used in the embodiment of the present invention is well known to the skilled person.
A kind of biological extraction process of the pineapple leaves of embodiment 1
As shown in Figure 1, the arghan raw material of the good drying of mechanical striking is impregnated with tap water, bath raio 1: 15-20, when
Between 20min.In power it is 200W with ultrasonic wave, after frequency handles 20min under the conditions of being 80kHz, is separated by solid-liquid separation, collects respectively super
Sound filtrate and fibrous raw material, it is spare.
The one ring DCE-01 strain of inclined-plane original seed lawn picking saved from this project group is seeded to 5.0mL improvement meat soup culture
Liquid sufficiently hangs even, 34 DEG C of culture 5.5h.Nutrient agar panel is coated on after dilution, 34 DEG C of culture 18h separate single colonie.It selects
Colonies typical is seeded to 5.0mL improvement cultured solution of broth, 34 DEG C of cultures 5h, as primary seed solution.Test tube liquid is seeded to Sheng
Have in the 100mL triangular flask of 50mLGR culture solution, mix well, 34 DEG C, 180r/min cultivates 5.5h, as secondary seed solution；
Secondary seed solution is seeded to again in the 500mL triangular flask for filling 200mL FJ culture solution by 2%, is mixed well, 34 DEG C,
180r/min cultivates 9h, obtains maturing fermentation liquid.
The maturing fermentation liquid of addition 2%, mixes well, prepares bacteria suspension in ultrasonic filtrate.Ultrasound is impregnated with bacteria suspension
Processed arghan raw material, 34 DEG C, 140r/min degumming 10-12h.
Laccase is diluted to final concentration of 500U/mL with the HAc-NH4Ac buffer of pH4-5.5,2,2- of addition joins nitrogen-two
(3- ethyl-benzothiazole -6- sulfonic acid) di-ammonium salts (ABTS) impregnate the degumming of DCE-01 bacterium by bath raio 1: 15 to final concentration 0.5%
Fibrous raw material afterwards, mixes well, and refines 3h under the conditions of 40 DEG C, is rinsed well with tap water, dehydration.Dewatered fiber is trembled
Pine, 1.5%BSK impregnate 10-20min, after de-oiling under the conditions of 50 DEG C low temperature drying to get the pineapple leaves for subsequent weaving
Fibrous finished product.
A kind of biological extraction process of the banana bast fiber of embodiment 2
The banana bast fiber raw material of the good drying of mechanical striking, bath raio 1: 20, time 25min are impregnated with tap water.With
Ultrasonic wave is that 200W is separated by solid-liquid separation after frequency handles 30min under the conditions of being 80kHz in power, collects ultrasonic filtrate and perfume (or spice) respectively
Any of several broadleaf plants bast fiber raw material, it is spare.
The one ring DCE-01 strain of inclined-plane original seed lawn picking saved from this project group is seeded to 5.0mL improvement meat soup culture
Liquid sufficiently hangs even, 34 DEG C of culture 5.5h.Nutrient agar panel is coated on after dilution, 34 DEG C of culture 18h separate single colonie.It selects
Colonies typical is seeded to 5.0mL improvement cultured solution of broth, and 34 DEG C of culture 5h obtain primary seed solution.Test tube liquid is seeded to Sheng
Have in the 100mL triangular flask of 50mLGR culture solution, mix well, 34 DEG C, 180r/min cultivates 5.5h, obtains secondary seed solution；
Secondary seed solution is seeded to again in the 500mL triangular flask for filling 200mLFJ culture solution, is mixed well, 34 DEG C, 180r/min training
9h is supported, maturing fermentation liquid is obtained.
The maturing fermentation liquid of addition 2%, mixes well, prepares bacteria suspension in ultrasonic filtrate.Ultrasound is impregnated with bacteria suspension
Processed banana bast fiber raw material, 34 DEG C, 140r/min degumming 10-12h.
Laccase is diluted to final concentration of 500U/mL with the HAc-NH4Ac buffer of pH4-5.5,2,2- of addition joins nitrogen-two
(3- ethyl-benzothiazole -6- sulfonic acid) di-ammonium salts (ABTS) impregnate the degumming of DCE-01 bacterium by bath raio 1: 20 to final concentration 0.5%
Banana bast fiber raw material afterwards, mixes well, and refines 4h under the conditions of 40 DEG C, is rinsed well with tap water, dehydration.After dehydration
Fiber shake up, 2%BSK impregnate 20min, after de-oiling under the conditions of 50 DEG C low temperature drying to get the perfume (or spice) for subsequent weaving
Any of several broadleaf plants bast fiber finished product.
Finally, being not intended to limit the scope of the present invention the above is only preferred embodiment of the invention.It is all this
Within the spirit and principle of invention, any modification, equivalent replacement, improvement and so on should be included in protection model of the invention
Within enclosing.
Claims (10)
1. the biological extraction process of fiber in a kind of waste agronomic crop bast raw material, which is characterized in that with water to waste agronomic crop
Bast raw material carries out immersion treatment, collects filtrate and fibrous raw material respectively after separation of solid and liquid, and DCE-01 is then added into filtrate
DCE-01 bacteria suspension is made in the maturing fermentation liquid of bacterium, and impregnates fibrous raw material degumming with DCE-01 bacteria suspension, then uses laccase/Jie
Body refining, is dehydrated after cleaning to obtain the final product.
2. biological extraction process according to claim 1, which is characterized in that the immersion degumming side of the DCE-01 bacteria suspension
Method are as follows: 33-35 DEG C, 120-150r/min degumming 10-15h.
3. biological extraction process according to claim 1 or 2, which is characterized in that described in the DCE-01 bacteria suspension
The concentration of DCE-01 bacterium is greater than 1 × 108cfu/mL。
4. biological extraction process according to claim 3, which is characterized in that the system of the maturing fermentation liquid of the DCE-01 bacterium
Preparation Method are as follows: DCE-01 strain is seeded in GR culture solution and is mixed, 5-6h is cultivated at 33-35 DEG C, is coated on after dilution
In YQ nutrient agar panel, single colonie is separated after cultivating 18-20h at 33-35 DEG C, colonies typical is selected and is seeded to GR culture solution
In and mix, at 33-35 DEG C and under the conditions of 150-180r/min, culture 5-6h obtains primary seed solution, then connects primary seed solution
Kind is to the GR culture solution of 6-10 times of volume, and culture 5-6h obtains secondary seed solution under the conditions of 33-35 DEG C and 150-180r/min, most
Secondary seed solution is seeded to the FJ culture solution of 20-50 times of volume afterwards, cultivates 8- under the conditions of 33-35 DEG C and 150-180r/min
10h to obtain the final product.
5. biological extraction process according to claim 4, which is characterized in that
The mass ratio formula of the GR culture solution are as follows: glucose 1%+ beef extract 0.5%+ peptone 0.5%+NaCl 0.5%,
It and with NaOH tune pH value is 7.0-7.5；
And/or the mass ratio formula of the YQ nutrient agar panel are as follows: glucose 0.5%+ nutrient agar 3.5%；
And/or the mass ratio formula of the FJ culture solution are as follows: glucose 2%+ Dried Corn Steep Liquor Powder 2%+ bean cake powder 1%+ peptone
0.01%+NaNO30.15%.
6. biological extraction process according to claim 1, which is characterized in that the method for the Laccase/Mediator refining are as follows: will
The HAc-NH of laccase pH value 4-5.54It is 500U/mL that Ac buffer, which is diluted to concentration, and 2,2- of addition joins (the 3- ethyl-benzene of nitrogen-two
And thiazole -6- sulfonic acid) di-ammonium salts are to concentration 0.2-0.5wt%, by the fibrous raw material after the immersion degumming of bath raio 1: 12-18, mixing
2-4h is reacted at 38-45 DEG C afterwards.
7. biological extraction process described in -6 according to claim 1, which is characterized in that the immersion treatment is ultrasound after water impregnates
Wave pretreatment, the bath raio of the immersion treatment are 1: 15-20, and the processing time is 15-30min.
8. biological extraction process according to claim 7, which is characterized in that the frequency and power of the ultrasonic wave be respectively
80-100KHz and 200-300W.
9. biological extraction process described in -8 according to claim 1, which is characterized in that further include place after cleaning and being dewatered
Reason, the post-processing successively include oil supply deoiling treatment and drying process；
Preferably, the method for the deoiling treatment are as follows: will clean and dewatered bast fiber shakes up and is placed on concentration as 1-
10-20min is impregnated in the BSK solution of 2wt%, the drying temperature of the drying process is lower than 50 DEG C.
10. biological extraction process according to claim 9, which is characterized in that the waste agronomic crop bast raw material is spinach
Trailing plants leaf bast raw material or banana stem raw material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910136851.1A CN109680345A (en) | 2019-02-25 | 2019-02-25 | The biological extraction process of fiber in a kind of waste agronomic crop bast raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910136851.1A CN109680345A (en) | 2019-02-25 | 2019-02-25 | The biological extraction process of fiber in a kind of waste agronomic crop bast raw material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109680345A true CN109680345A (en) | 2019-04-26 |
Family
ID=66196689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910136851.1A Pending CN109680345A (en) | 2019-02-25 | 2019-02-25 | The biological extraction process of fiber in a kind of waste agronomic crop bast raw material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109680345A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115486306A (en) * | 2022-09-15 | 2022-12-20 | 湖南艾布鲁环保科技股份有限公司 | Agricultural residue fiber plant-growing net and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235357A (en) * | 2007-09-30 | 2008-08-06 | 中国农业科学院麻类研究所 | Industrial fermentation ramee rapid extraction technique for Erwinia |
| CN107268091A (en) * | 2017-06-30 | 2017-10-20 | 中国农业科学院麻类研究所 | A kind of bluish dogbane bast Degumming method |
-
2019
- 2019-02-25 CN CN201910136851.1A patent/CN109680345A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235357A (en) * | 2007-09-30 | 2008-08-06 | 中国农业科学院麻类研究所 | Industrial fermentation ramee rapid extraction technique for Erwinia |
| CN107268091A (en) * | 2017-06-30 | 2017-10-20 | 中国农业科学院麻类研究所 | A kind of bluish dogbane bast Degumming method |
Non-Patent Citations (5)
| Title |
|---|
| 冯湘沅等: "DCE01菌株降解大麻韧皮的发酵液成分变化规律", 《中国麻业科学》 * |
| 吴洁: "《绢麻纺概论》", 31 October 2001, 中国纺织出版社 第1版 * |
| 张勇兵: "漆酶及漆酶介体体系处理对黄麻纤维中木质素结构影响的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 邓一民等: "《天然纺织纤维加工化学》", 30 September 2010, 西南师范大学出版社 第1版 * |
| 陆强等: "《液体生物燃料技术与工程》", 31 January 2013, 上海科学技术出版社 第1版 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115486306A (en) * | 2022-09-15 | 2022-12-20 | 湖南艾布鲁环保科技股份有限公司 | Agricultural residue fiber plant-growing net and preparation method and application thereof |
| CN115486306B (en) * | 2022-09-15 | 2024-01-26 | 湖南艾布鲁环保科技股份有限公司 | Agricultural residue fiber plant net and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100529201C (en) | Microbiological degumming method for mulberry fibre | |
| CN102174399A (en) | Method for preparing herbal biological fiber and biological paper pulp | |
| CN101597575A (en) | Bio-pulping composite bacteria microbial dry powder and environment-friendly and energy-efficient composite bacteria bio-pulping process | |
| CN101787571A (en) | Resource utilization process for clean hemp biological degumming and waste thereof | |
| CN101880637A (en) | Bacillus subtilis and application thereof in sisal hemp degumming | |
| CN108457113A (en) | The natural pure filametntary method of microbial degradation extraction is carried out to herbaceous plant | |
| CN102409411B (en) | Method for preparing fibrilia by using epicoccum nigrum DB3 strains | |
| CN107287975A (en) | A kind of processing method of discarded leather | |
| CN103835004B (en) | A kind of reparation technology of abutilon fibre | |
| CN105568397B (en) | A kind of Degumming method of ramie | |
| CN109680345A (en) | The biological extraction process of fiber in a kind of waste agronomic crop bast raw material | |
| CN110156913A (en) | A method of discarded shrimp and crab shells chitin extraction is handled using bacillus cereus | |
| CN101423983A (en) | Bacteria mixing and degumming method of ramie phloem fiber | |
| CN112094759B (en) | Method for preparing white rot fungus liquid culture medium by utilizing tanning wastes and application | |
| CN101684571A (en) | Method for biologically degumming bast fibers | |
| CN105401224A (en) | Environment-friendly and degradable excellent modified bamboo fiber | |
| CN101805725B (en) | Complex enzyme generated by aspergillus niger and application thereof in boiling-off of flax roving | |
| CN102409413B (en) | Method for preparing fibrilia by using penicillium purpurogenum DB1 strains | |
| CN109989114A (en) | A kind of method of flax degumming | |
| CN102329736B (en) | Alternaria alternata DB2 strain and preparation and application thereof | |
| CN113789576A (en) | Flax biological degumming method based on solid fermentation method and alkaline composite strain system | |
| CN102337220B (en) | Penicillium purpurogenum DB1 strain and preparation and application thereof | |
| Yinghua et al. | Production of efficient enzymes for flax retting by solid state fermentation with Aspergillus niger | |
| CN100535202C (en) | Microbiological degumming method for smartweed fibre | |
| CN105463861A (en) | Biological flax roving degumming method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190426 |
|
| RJ01 | Rejection of invention patent application after publication |