CN109675094A - A kind of absorbable bone wax and preparation method thereof with hemostatic function - Google Patents
A kind of absorbable bone wax and preparation method thereof with hemostatic function Download PDFInfo
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- CN109675094A CN109675094A CN201710968793.XA CN201710968793A CN109675094A CN 109675094 A CN109675094 A CN 109675094A CN 201710968793 A CN201710968793 A CN 201710968793A CN 109675094 A CN109675094 A CN 109675094A
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- Prior art keywords
- bone wax
- linked
- hyaluronic acid
- solids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/02—Surgical adhesives or cements; Adhesives for colostomy devices containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Abstract
The invention discloses a kind of absorbable bone wax with hemostatic function, raw material are made of solids and water, and the solids includes cross-linked-hyaluronic acid, calcium phosphate and soluble starch, and the weight of the water is the 20%-100% of solids weight；The weight ratio of the solids are as follows: cross-linked-hyaluronic acid: calcium phosphate: soluble starch is (20-70): (20-50): (0-10).Bone wax of the invention is directed to traditional bone wax poor biocompatibility, non-degradable, the defect for influencing sclerotin healing, is retaining the good haemostatic effect of traditional bone wax simultaneously, can in vivo natural degradation without causing residue advanced stage related complication.
Description
Technical field
The present invention relates to technical field of biomedical materials, and in particular to a kind of absorbable bone wax with hemostatic function and
Preparation method.
Background technique
Destruction of bone can be often related in the surgical procedures such as orthopaedics, thoracic surgery, neurosurgery, lead to bone face oozing of blood.Due to
Blood supply is abundant, and this bleeding is difficult to stop by conventional methods such as coagulation, clamp, hemostatic gauze, gelfoam fillings, therefore clinical normal
Cancellous bone surface of a wound hemostasis is carried out with bone wax.The main component of traditional bone wax is beeswax, vaseline etc., and hemostatic mechanism is to pass through machine
Bleeding bone face is clogged and is sealed in the physical barriers effect of tool, and hemostasis quickly, is clinically widely used.But traditional bone wax biology
Poor compatibility, it is difficult to by body degradation, absorb, residual can cause the severe complications such as foreign body reaction, wound infection, state in vivo
It is outer even have oppress the report that spinal cord causes lower limb paralysis because the bone wax used falls off；Simultaneously traditional bone wax can hinder blood at
The aggregation for dividing (fibrin and blood platelet), is unfavorable for the formation of primary callus, seriously affects the normal healing of bone.Therefore, change
Into the biocompatibility of bone wax, allows to natural degradation in vivo under the premise of not influencing its performance and absorb with important meaning
Justice.
Summary of the invention
For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of adsorbable bones with hemostatic function
Wax.
The second object of the present invention is to provide a kind of preparation method of absorbable bone wax with hemostatic function.
To achieve the goals above, present invention firstly provides a kind of absorbable bone wax with hemostatic function, raw material by
Solids and water composition, the solids includes cross-linked-hyaluronic acid, calcium phosphate and soluble starch, and the weight of the water is solid
The 20%-100% of body object weight；The weight ratio of the solids are as follows: cross-linked-hyaluronic acid: calcium phosphate: soluble starch is
(20-70): (20-50): (0-10).Preferably, the water is deionized water.
Preferably, the absorbable bone wax, the weight ratio of the solids are as follows:
Cross-linked-hyaluronic acid: calcium phosphate: soluble starch is (30-60): (35-50): (1-10).
Preferably, the absorbable bone wax, raw material include the composition of following weight percent: cross-linked-hyaluronic acid
33.3%, calcium phosphate 30%, soluble starch 3.3%, water 33.4%.Preferably, the water is deionized water.
Preferably, the molecular weight of the cross-linked-hyaluronic acid is 100,000-100 ten thousand.
Further, the preparation method of the present invention also provides a kind of absorbable bone wax with hemostatic function, feature
It is comprising following steps:
(1) each raw material component is weighed in proportion；
(2) cross-linked-hyaluronic acid and calcium phosphate are placed in reaction vessel, are sufficiently mixed uniformly by stirring；
(3) it adds water in soluble starch to stir and heat and makes its pregelatinated, then by the soluble starch of pregelatinated
It is added in the reaction vessel of step (2) and is sufficiently mixed uniformly with other raw materials up to the bone wax.
Preferably, the stirring in the step (2) is magnetic agitation.
Preferably, the heating temperature in the step (3) is 60-70 DEG C.
Preferably, the step further includes by the bone wax sealed package, ultraviolet disinfection.
Beneficial effect
Bone wax product of the present invention has preparation process simple, biology while the traditional bone wax of reservation good haemostatic effect
Compatibility is good, in vivo degradable absorption and the advantages of noresidue.
Detailed description of the invention
The electron-microscope scanning figure of 1 bone wax material of Fig. 1 embodiment；
The electron-microscope scanning figure of 2 bone wax material of Fig. 2 embodiment；
The electron-microscope scanning figure of 3 bone wax material of Fig. 3 embodiment；
Fig. 4 CCK-8 experimental result.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, reagent used can be commercially available.
Test method without specific conditions in embodiment, usually conventional method in that art or according to reagent manufactory
Condition proposed by family.
Embodiment 1
1, ratio weighs each raw material component in parts by weight: cross-linked-hyaluronic acid 33.3%, calcium phosphate 33.3%, deionization
Water 33.4%；
2, cross-linked-hyaluronic acid and calcium phosphate are placed in reaction vessel, after being sufficiently mixed uniformly by magnetic agitation, are added
Enter deionized water to stir up to the bone wax；
3, by gained bone wax sealed package, ultraviolet disinfection.
Result of the obtained material through electron-microscope scanning is as shown in Figure 1, be the characterization scanned at 20um, 50um respectively.
Embodiment 2
1, ratio weighs each raw material component in parts by weight: cross-linked-hyaluronic acid 33.3%, calcium phosphate 30%, and solubility is formed sediment
Powder 3.3%, deionized water 33.4%；
2, cross-linked-hyaluronic acid and calcium phosphate are placed in reaction vessel, after being sufficiently mixed uniformly by magnetic agitation；
3, will be stirred and heated to 60-70 DEG C in deionized water addition soluble starch makes its pregelatinated, is then added
Enter in the reaction vessel of step 2 and is sufficiently mixed uniformly with other raw materials up to the bone wax；
4, by gained bone wax sealed package, ultraviolet disinfection.
Result of the obtained material through electron-microscope scanning is as shown in Fig. 2, be the characterization scanned at 20um, 50um respectively.
Embodiment 3
1, ratio weighs each raw material component in parts by weight: cross-linked-hyaluronic acid 33.3%, calcium phosphate 26.7%, soluble
Starch 6.7%, deionized water 33.3%；
2, cross-linked-hyaluronic acid and calcium phosphate are placed in reaction vessel, after being sufficiently mixed uniformly by magnetic agitation, are added
Enter deionized water；
3, will be stirred and heated to 60-70 DEG C in deionized water addition soluble starch makes its pregelatinated, is then added
Enter in the reaction vessel of step 2 and is sufficiently mixed uniformly with other raw materials up to the bone wax；
4, by gained bone wax sealed package, ultraviolet disinfection.
Result of the obtained material through electron-microscope scanning is as shown in figure 3, be the characterization scanned at 20um, 50um respectively.
Embodiment 4CCK-8 experiment
1, by 100ul human marrow mesenchymal stem cell (be purchased from 307 ivy translational medicine centers) suspension, (density is
5x104A cell/ml) 96 orifice plates are inoculated in, culture plate is placed in 37 DEG C, 5%CO2It is cultivated under 95% damp condition, culture medium
The next day replace.
2, tri- kinds of different materials of embodiment 1-3 are respectively labeled as sample 1, sample 2, sample 3, by 3 sample bone wax and pass
Bone wax (0.1mg is spherical, German She Pai group, 1029754 type bone wax) of uniting sterilizes 8 hours through ultraviolet irradiation, observes to 24 hours
It is respectively placed in after cell is adherent in culture plate and is set as experimental group (including sample 1, sample 2, sample 3) and Normal group.Inoculation 1
It, 3 days, 5 days and after 7 days, surveyed using Cell counting Kit -8 (CCK-8, eastern Renhua subject skill (Shanghai) Co., Ltd.)
Determine the adherency situation of cell.According to the ratio (CCK-8 reagent: culture medium) of 1:9 by the CCK-8 of 100ml and culture medium mixed liquor
It is added in each hole, in 37 DEG C, 5%CO2Hatch 2 hours under 95% damp condition.Then each hole is read using microplate reader
Absorbance at 450 nm.
3, result as shown in figure 4, with the time growth, numerical value is increasing, illustrates bone wax prepared by embodiment 1-3
Sample is to cytotoxic, and compared to traditional bone wax, bone wax sample cell Proliferation prepared by embodiment 1-3 is more preferable, wherein sample
Cell Proliferation in sheet 2 is best.
5 degradation experiment of embodiment
Respectively by three kinds of different materials in embodiment 1-3 be made diameter be 1cm, highly be 0.2cm bone wax material, respectively
It is designated as sample 1, sample 2, sample 3, three sample bone wax and traditional bone wax (German She Pai group, 1029754 type bone wax) are simultaneously
It is soaked in 20mlPBS solution, (37 DEG C, 60rpm) are vibrated in constant temperature deionization water-bath.Every 24 hours replacement PBS solutions, respectively at
Retained material weight percent is measured at 1 day, 3 days, 5 days, 7 days, the results are shown in Table 1, and the bone wax material of sample 1-3 can be
It is effectively degraded in one week, wherein the degradation speed of sample 2 is faster more thorough.
The degradation of 1 material of table
In conclusion the bone wax of embodiment 1-3 preparation has better biocompatibility compared to traditional bone wax, in vivo may be used
Degradation absorbs and noresidue.Wherein, raw material weight percentage are as follows: cross-linked-hyaluronic acid 33.3%, calcium phosphate 30%, solubility are formed sediment
The bone wax biocompatibility of powder 3.3%, water 33.4% is best, degradable can absorb.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a kind of absorbable bone wax with hemostatic function, which is characterized in that its raw material is made of solids and water, the solid
Object includes cross-linked-hyaluronic acid, calcium phosphate and soluble starch, and the weight of the water is the 20%-100% of solids weight；Institute
State the weight ratio of solids are as follows:
Cross-linked-hyaluronic acid: calcium phosphate: soluble starch is (20-70): (20-50): (0-10).
2. absorbable bone wax as described in claim 1, which is characterized in that the weight ratio of the solids are as follows:
Cross-linked-hyaluronic acid: calcium phosphate: soluble starch is (30-60): (35-50): (1-10).
3. absorbable bone wax as described in claim 1, which is characterized in that its raw material includes the composition of following weight percent:
4. absorbable bone wax as claimed in any one of claims 1-3, which is characterized in that the molecule of the cross-linked-hyaluronic acid
Amount is 100,000-100 ten thousand.
5. the preparation method of the absorbable bone wax as described in claim 1-4 any one, which is characterized in that it includes following step
It is rapid:
(1) each raw material component is weighed in proportion；
(2) cross-linked-hyaluronic acid and calcium phosphate are placed in reaction vessel, are sufficiently mixed uniformly by stirring；
(3) it adds water in soluble starch to stir and heat and makes its pregelatinated, then the soluble starch of pregelatinated is added
It is sufficiently mixed uniformly with other raw materials up to the bone wax in the reaction vessel of step (2).
6. preparation method as claimed in claim 5, which is characterized in that the stirring in the step (2) is magnetic agitation.
7. preparation method as claimed in claim 5, which is characterized in that the heating temperature in the step (3) is 60-70 DEG C.
8. preparation method as claimed in claim 5, which is characterized in that the step further include by the bone wax sealed package,
Ultraviolet disinfection.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710968793.XA CN109675094A (en) | 2017-10-18 | 2017-10-18 | A kind of absorbable bone wax and preparation method thereof with hemostatic function |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710968793.XA CN109675094A (en) | 2017-10-18 | 2017-10-18 | A kind of absorbable bone wax and preparation method thereof with hemostatic function |
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| CN109675094A true CN109675094A (en) | 2019-04-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112138205A (en) * | 2019-06-26 | 2020-12-29 | 广东博与再生医学有限公司 | Bone hemostatic material |
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| JP2004081739A (en) * | 2002-08-29 | 2004-03-18 | Mitsuru Akashi | Composition for hemostasis of hydroxy apatite polymer composite material |
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| CN101444635A (en) * | 2009-01-05 | 2009-06-03 | 北京化工大学 | Degradable bone lacuna inside haemostatic material and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112138205A (en) * | 2019-06-26 | 2020-12-29 | 广东博与再生医学有限公司 | Bone hemostatic material |
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Application publication date: 20190426 |