CN109666675A - Brown paddy plant hopper NlAtg3 gene, coding albumen and its application - Google Patents
Brown paddy plant hopper NlAtg3 gene, coding albumen and its application Download PDFInfo
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- CN109666675A CN109666675A CN201811388131.6A CN201811388131A CN109666675A CN 109666675 A CN109666675 A CN 109666675A CN 201811388131 A CN201811388131 A CN 201811388131A CN 109666675 A CN109666675 A CN 109666675A
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- A—HUMAN NECESSITIES
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
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- Physics & Mathematics (AREA)
- Insects & Arthropods (AREA)
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Abstract
The invention discloses a kind of brown paddy plant hoppersNlAtg3Gene, coding albumen and its application.NlAtg3Gene, for nucleotide sequence as shown in SEQ ID NO:1, which is that brown paddy plant hopper normal existence institute is required, and function, which is suppressed, will lead to the decline of brown paddy plant hopper survival rate.NlAtg3The albumen of gene coding, amino acid sequence is as shown in SEQ ID NO:2.DescribedNlAtg3The application of gene or albumen is used for agricultural chemicals research and development and biological control brown paddy plant hopper.The present invention is to gene successful implementation RNA interference, the results showed that the survival rate of pest declines, and is expected to give full play to the function of bionomic control while realizing inhibition pest.
Description
Technical field
The present invention relates to a kind of brown paddy plant hopper NlAtg3 gene, coding albumen and its applications.
Background technique
Brown paddy plant hopper (Nilaparvata lugensSt l) it is a kind of monophagy rice grub, mainly pass through feeding rice
Phloem sap is caused harm rice.Currently, the emergence control to brown paddy plant hopper is mainly controlled by chemical pesticides, but chemical prevention is easy to lead
The drug resistance of pest is caused to rise, on rice the problems such as harmful substance residual, pest resurgence and environmental pollution.In prevention and treatment brown paddy plant hopper
Pesticide in, once played effective pesticide of better effects, such as Fipronil, imidacloprid, Buprofezin, cleaned out or
Limitation uses, to find out its cause, such chemical pesticide mostly kills pest as target, still, due to brown paddy plant hopper using extensive high intensity
The extremely complex equal practical reasons of population genetic diversity, wherein often have some individuals that can survive, high selection pressure
Exercising result eventually results in population more adaptable and is formed.On the other hand, chemical pesticide is while killing off the insect pests,
The non-target organism including natural enemy can be jeopardized, inevitably adversely affected to farmland ecosystem, cause to give birth to
State prevention and treatment function cannot get due performance.Therefore, it screens and prevents and treats target with new brown paddy plant hopper is found, adjust current brown paddy plant hopper
Control strategy has important practical significance.
CN107988244A discloses a kind of brown paddy plant hopper and survives relevant ATPSb gene, coding albumen and its application, the base
Because playing critical function in maintenance brown paddy plant hopper normally survives, function, which is suppressed, will lead to the decline of brown paddy plant hopper survival rate.
Summary of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of brown paddy plant hopper NlAtg3 gene, coding albumen and its
Using.The present invention according toNlAtg3The protein of gene coding is relatively conservative but nucleic acid sequence and other biological homology are lower
Feature carries out RNA interference to target gene, realizes the inhibition in nucleic acid level to brown paddy plant hopper, reduce brown paddy plant hopper significantly
Survival rate, and avoiding may be to killing caused by non-target organism in spraying pesticide etc. that protein level uses.
Technical scheme is as follows:
A kind of brown paddy plant hopperNlAtg3Gene, for nucleotide sequence as shown in SEQ ID NO:1, which encodes ATG3 albumen, is
Brown paddy plant hopper normal existence institute is required, and function, which is suppressed, will lead to the decline of brown paddy plant hopper survival rate.
A kind of brown paddy plant hopperNlAtg3The albumen of gene coding, amino acid sequence is as shown in SEQ ID NO:2, the base
It will lead to the decline of brown paddy plant hopper survival rate because function is suppressed.
A kind of brown paddy plant hopperNlAtg3The application of gene is used for agricultural chemicals research and development and biological control brown paddy plant hopper.
It is a kind of for the brown paddy plant hopperNlAtg3Application of the RNA perturbation technique of gene in terms of controlling brown paddy plant hopper, institute
The RNA perturbation technique stated causes the survival rate of brown paddy plant hopper to decline.
A kind of brown paddy plant hopper as mentionedNlAtg3The application of the albumen of gene coding, it is brown for agricultural chemicals research and development and biological control
Plant hopper.
Beneficial effects of the present invention: (1) brown paddy plant hopper survival rate declines, and can mitigate pests to the direct of rice crop
Harm.(2) nucleotide sequence and natural enemy nucleotide sequence homology lower feature of the present invention using target gene, Ke Yi
RNA interference is carried out in nucleic acid level, is avoided due to the conservative injury to non-target organisms such as natural enemies of protein structure, is expected to
While realizing inhibition pest, the function of bionomic control is given full play to.
Detailed description of the invention
Fig. 1 is brown paddy plant hopperNlAtg3The mRNA expression of gene.Wherein, 1-2N:1-2 age nymph；3-4N:3-4 age
Nymph；5N:5 age nymph；E1-9: emergence 1-9 days female brown paddy plant hoppers.
Fig. 2 is RNA interference to brown paddy plant hopperNlAtg3The influence of gene expression amount；
Data are 3 duplicate average value ± standard deviations in figure, asterisk indicate on statistical analysis processing group and control group it
Between there are extremely significant difference (TIt examines,P< 0.01).DsGFP: control group；dsNlAtg3:dsNlAtg3The RNA interference group of feeding.
Fig. 3 isNlAtg3The RNA of gene interferes the influence to brown paddy plant hopper survival rate；
Data are 3 duplicate average value ± standard deviations in figure, asterisk indicate on statistical analysis processing group and control group it
Between there are significant difference (TIt examines,P< 0.05), double asterisk indicates to exist between processing group and control group on statistical analysis significant
Difference (TIt examines,P< 0.01).
Specific embodiment
ATPSb gene is a subunit of atp synthase in mitochondria, and atp synthase is responsible for the synthesis of ATP in mitochondria
Journey, the disturbed synthesis (CN107988244A) that will affect ATP in mitochondria of this gene function.
NlAtg3Gene is the gene that ATG3 albumen is encoded in brown paddy plant hopper, and ATG3 albumen is cell autophagy (Autophagy)
One of generating process ubiquitin-like E2 albumen, it is ethanolaminated that it is catalyzed ATG8 protein, phospholipid acyl
(Phosphatidylethanolamine, PE).Cell autophagy is a kind of cell autophagy row for being prevalent in organism
To be the significant process of evolution conservative in eucaryote being had enough to meet the need to intracellular matter.The egg of some damages during this
White or organelle is sent into lysosome by after autophagy vesicle (autophagosome) package of double membrane structure and is degraded and followed
Ring utilizes.There are many cell autophagy albumen (Autophagy-related gene, ATG) to participate in for the formation of cell autophagy corpusculum
And the degradation of target protein is finally completed with lysosome fusion.Cell autophagy has played important function in insect,NlAtg3Gene is interfered, and will affect the autophagy process of insect cell, and then influence the normal growth and development of insect.
So ATPSb gene participate in be ATP in cell mitochondrial synthesis,NlAtg3What gene participated in is cell
Autophagy process, two genes adhere to different cellular physiological processes separately, and as the different targets of brown paddy plant hopper prevention and treatment, the two has essence
It is different.
Below in conjunction with the drawings and specific embodiments, the present invention is described further.
Embodiment 1
1 materials and methods
1.1 for trying brown paddy plant hopper
It is to feel the Tn population raised on worm rice varieties TN1 for examination Population of Rice Brown Planthopper, continuously raises 60 on TN1 by this laboratory
More than generation, raising temperature is 26 ± 2 DEG C, and relative humidity is 80% ± 5%, photoperiod 12L:12D.
Main agents
TaKaRa MiniBEST Universal RNA Extraction Kit, TaKaRa MiniBEST Agarose Gel
DNA Extraction Kit, PrimeScript RT reagent Kit With gDNA Eraser, DNA 2000
Marker,Premix Taq(TaKaRaTaq2.0 plus dye of Version), SYBR Premix Ex Taq is equal
Purchased from Dalian TaKaRa company, 5 '/3 ' Kit User Manual of SMARTer RACE is purchased from U.S. Clontech company,
MEGAscript T7 High Yield Transcription Kit is purchased from U.S. Ambion company, and sequencing and primer close
It is completed at having by Shanghai Sani Biotechnology Co., Ltd.
Brown paddy plant hopperNlAtg3The clone of full length gene cDNA
The brown paddy plant hopper nymph of different larval instar and adult mixing material on TN1 rice are collected, is immediately placed in liquid nitrogen and freezes.When sampling
Nymph takes 30-50, and only adult takes 5.It is extracted using TaKaRa MiniBEST Universal RNA Extraction Kit
Total serum IgE, specific steps are carried out referring to its specification.Use agarose gel electrophoresis and Nanodrop 2000(Thermo) to RNA
Carry out integrality and purity detecting.Using 1 μ g total serum IgE as template, PrimeScript RT reagent Kit With is used
GDNA Eraser kit reverse transcription synthesize cDNA, be stored in -20 DEG C it is spare.
According to the transcript profile sequencing sequence information in this laboratory, the part core sequence of brown paddy plant hopper NlAtg3 gene is obtained,
And it is identified through the website NCBI sequence alignment.Using 5.0 software Design primers of Primer PremierNlAtg3- F andNlAtg3-R
(table 1), verifies core sequence.Using the mixing brown paddy plant hopper cDNA of different larval instar on TN1 as template, its core sequence is verified
Column.NlAtg3For the clone of full-length cDNA referring to the method for Hao et al(2015), pcr amplification reaction system is 50 μ L, wherein
Including 25 μ L of PCR Mix, 2 19 μ L of μ L, ddH2O of each 2 μ L, cDNA template of 10 μm of positive anti-primers of ol/L.PCR reaction interval
Sequence are as follows: 94 DEG C of 4 min；94 DEG C of 30 s, 55 DEG C of 30 s, 72 DEG C of 3 min, 30 circulations；72℃ 10 min；4 DEG C of preservations.
Pcr amplification product is detected through 1% agarose gel electrophoresis, with TaKaRa MiniBEST Agarose Gel DNA
Extraction Kit recycles target fragment, is connected to upper 4 DEG C of carrier pMD-18T overnight, is transformed into JM109 competent cell
Add 1 mL LB liquid medium, 37 DEG C of 2 h of shaking table culture, 200 μ L bacterium solutions is taken to be coated on the LB solid medium containing 1% Amp
9 h are cultivated in 37 DEG C of inversions, and 5 single bacteriums of random picking fall within the 1.5 mL centrifuge tubes of 1 mL of LB fluid nutrient medium containing 1% Amp
In 37 DEG C of 12 h of shaking table culture, take 1 μ L bacterium solution to carry out bacterium solution PCR identification and positive colony bacterial strain and be sent to Shanghai Sani's biology section
The sequencing of skill Co., Ltd.Sequencing result DNAMAN software and former sequence alignment verifying.
Satisfactory RNA sample is chosen, synthesizes 5'- with 5 '/3 ' Kit User Manual of SMARTer RACE
The template of RACE and 3'-RACE.Separately design outer primerNlAtg3- 5O andNlAtg3- 3O(table 1) and inner primerNlAtg3-
5I andNlAtg3- 3I(table 1).It according to RACE kit specification, is expanded using 2 ends of the nest-type PRC to target gene, electricity
Swimming, glue recycling, connects, and converts, and sequencing, sequencing result application DNAMAN software is compared splicing and obtainsNlAtg3Overall length
cDNA.According to the full length cDNA sequence of acquisition, designs overall length and verify primerNlAtg3- FL-F andNlAtg3- FL-R(table 1) to spelling
The full length sequence connect is verified.
1 gene cloning of table, quantitative fluorescent PCR and the primer for synthesizing dsRNA
1.4 brown paddy plant hopperNlAtg3The sequence of gene is analyzed
It is obtained according to DNAMAN splicingNlAtg3Full-length cDNA sequence information analyzes software (ORF using open reading frame
Finder https: //www.ncbi.nlm.nih.gov/orffinder/) open reading frame and protein translation feelings are predicted
Condition, using NCBI Blastx carry out amino acid sequence homology comparison, using online tool ExPASy (http: //
Web.expasy.org/protparam/) molecular weight of protein, theoretical isoelectric point etc. are predicted, using SignalP
4.1 server (http://www.cbs.dtu.dk/services/SignalP/) predict signal peptide, using
The Line tool InterProScan (http://www.ebi.ac.uk/interpro/search/sequence-search) is right
Protein functional domain is predicted.
Brown paddy plant hopperNlAtg3The expression rule of gene is analyzed
Utilize the opposite table of fluorescent quantitative PCR technique detection NlAtg3 gene Population of Rice Brown Planthopper of different larval instar on TN1 rice
Up to amount, including 1-2 age, 3-4 age, 5 age nymphs and the female adult pest sprouted wings 1,3,5,7,9 day and male worm.Quantitative fluorescent PCR is special
Property primer be QNlAtg3- F and QNlAtg3- R(table 1), using RPS11 gene as internal reference (Yuan et al, 2014), detect brown
Plant hopperNlAtg3The relative expression quantity of gene.Quantitative fluorescent PCR refers to the reaction system and method for horse gorgeous equal (2013), wherein moving back
Fiery temperature is changed to 53 DEG C.
Feeding method carries out RNAi
Primer ds according to cDNA full length sequence designed for synthesis dsRNA interference fragmentNlAtg3- F and dsNlAtg3- R(table
1), and at the end specific primer 5' add protection base (GGATCC) and T7 promoter (TAATACGACTCACTATA).
In order to not influence the transcriptional level detection after RNAi, interference fragment does not include the segment of fluorescence quantitative PCR detection.According to
MEGAscript T7 High Yield Transcription Kit(Ambion) kit specification synthesis dsRNA.It closes
DsRNA after is purified using the LiCl precipitation method: being added the ddH2O's and 30ul of 30ul into dsRNA reaction system
LiCl Precipitation Solution, -20 DEG C of placement 1h or so, 11000rpm, 4 DEG C of centrifugation 15min, go after supernatant plus
Enter and washed with the 70% ethyl alcohol 1ml that DEPC water is prepared, ethyl alcohol is removed after centrifugation, 20ulddH is added2O dissolution, places -20
DEG C etc. it is to be used.
Raising 2 age brown paddy plant hopper nymphs on TN1 rice varieties are taken to carry out artificial feeding, rearing conditions are referring to Fu etc.
(2001) nutrient solution prescription, feeding device are the bilateral glass tube (2.5 that both ends cover Parafilm film (including feeding liquid)
The cm of cm × 15), after test worm feeds adaptation in feeding liquid 5 days, RNA interference processing is carried out, the dsRNA feeding liquid of purifying is matched
Make final concentration of 0.5 μ g/ μ L.When processing, using the dsRNA dilution of feeding liquid addition respective volume as control group (CK), often
Feeding device accesses 20 by above-mentioned pretreatment and develops almost the same brown paddy plant hopper nymph, and 3 repetitions are arranged, replace daily
Feeding liquid, the brown paddy plant hopper of cleaning and statistics death, calculates survival rate.
In addition, the RNA interference processing that setting is parallel, every feeding device is put into 25 consistent brown paddy plant hopper nymphs of development, and
Two pipes are sampled each group in every 2 days respectively as a repetition, and every group takes 6 nymphs, and carry out the extracting of RNA and subsequentNlAtg3The fluorescence quantitative PCR detection of gene expression amount.
Data statistics and analysis
Data preparation is carried out using WPS Excel software, with SPSS16.0 independent sampleTIt examines and carries out significant difference point
Analysis.
As a result with analysis
2.1 brown paddy plant hopperNlAtg3The cDNA full-length clone and sequence of gene are analyzed
The brown paddy plant hopper autophagy related gene obtained with biological software analysisNlAtg3The cDNA full length sequence of gene.Use ORF
Finder analysis finds,NlAtg3The ORF of gene totally 990 bp(SEQ ID NO:1), encode 329 amino acid (SEQ ID
NO:2).ExPASy software analysis is foundNlAtg3The molecular weight of albumen of gene coding is 37.78 kDa, and isoelectric point (pI) is
4.46.Protein structure domain prediction discoveryNlAtg3Gene has the typical end N- (being located at 8-158 amino acids) and the end C-
End structure (is located at 300-323 amino acids).
Brown paddy plant hopperNlAtg3The expression rule of gene is analyzed
Utilize fluorescence quantitative PCR detectionNlAtg3Gene brown paddy plant hopper different developmental phases expression rule, as a result, it has been found that,NlAtg3Gene has expression in brown paddy plant hopper different developmental phases, and 1-2 age nymph is relatively high, and 3-4 age is placed in the middle, 5 ages minimum (figure
1, left)；Each stage of development variation of female adult is unobvious (Fig. 1, right).
Interference is to brown paddy plant hopperNlAtg3The influence of gene expression dose
Fluorescence quantitative PCR detection injects the brown paddy plant hopper after the dsRNA 4d of 5 μ g/ μ l, the results show thatNlAtg3Gene injection
Expression after dsRNA declines to a great extent, and compared with dsGFP control group, has reached pole significant difference (P < 0.01) (figure
2).
2.4 NlAtg3The RNA of gene interferes the influence to brown paddy plant hopper survival rate
RNA interference as a result, it has been found that, inject ds NlAtg3Lethal effect it is significant, RNA interference the 4th day, inject dsNlAtg3Processing group survival rate and control group reached significant difference (P < 0.05), just reached with control group since the 5th day
Extremely significant difference (P < 0.01) is arrived, survival rate only has 6.67%(Fig. 3 when by the 7th day).
Sequence table
<110>China's metering university
<120>brown paddy plant hopper NlAtg3 gene, coding albumen and its application
<141> 2018-11-21
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1002
<212> DNA
<213>brown paddy plant hopper (Nilaparvata lugens Stal)
<400> 1
atgcaaagtg ttattaatac tgtaaaaggg actgctctcg gtgttgctga gtatttaaca 60
cctgttttaa aggaatcgaa atttcgtgag acgggagtga tcactcctga agagtttgtg 120
gcggccggcg atcacctggt gcatcactgc cccacctggc agtgggcgtc cggggacgaa 180
gccagggcca agacctactt gcccaagacc aaacagtttc tcatcaccaa gaatgtgccc 240
tgctcacgaa ggtgcaaaga gattgagtat tgtgacgagc aagagaaaat tctagagcct 300
gatgatcctg acggtggctg ggtggacact caccgcgtgg accccactag tggcctcgac 360
gaaaatgtct ccggaatgac attagactca aacacaactg ttcagagggt caatgattca 420
cctccatctg gagactttca gacgaacacc acaaacgcca ataataatgt cgatgatgat 480
gatgatgacg atgatgataa cgaagaagca gccgacatgg aaatgtttga ggaaagtggt 540
cttctagacg aggaggatga ggcgactgct gaggatgtca aaatcgaaaa agatgaaaga 600
aatgctgcag gtgacggcga gatagtcaaa accagaacgt atgacttaca tatcacatac 660
gacaaatatt accagactcc gcgattatgg ctttttggtt atgatgagaa ccataagcca 720
ctgaatgttg aacagatgta tgaagatgtg aatcaagatt atgcgaagaa aactgtgaca 780
atggaaacac atccgcacgt tccagggcct cccatggctt ctgtccatcc ttgcaggcac 840
gctgaagtga tgcagaagat aattcagacc gtattggaag gaggaggcga gctaggtgtt 900
catatgtatt tgataatttt tctgaaattt gttcaatcag tcattccaac tatagagtat 960
gattacacac aaaattttac aatgtggtct aaaacgctgt ga 1002
<210> 2
<211> 333
<212> PRT
<213>brown paddy plant hopper (Nilaparvata lugens Stal)
<400> 2
Met Gln Ser Val Ile Asn Thr Val Lys Gly Thr Ala Leu Gly Val Ala
1 5 10 15
Glu Tyr Leu Thr Pro Val Leu Lys Glu Ser Lys Phe Arg Glu Thr Gly
20 25 30
Val Ile Thr Pro Glu Glu Phe Val Ala Ala Gly Asp His Leu Val His
35 40 45
His Cys Pro Thr Trp Gln Trp Ala Ser Gly Asp Glu Ala Arg Ala Lys
50 55 60
Thr Tyr Leu Pro Lys Thr Lys Gln Phe Leu Ile Thr Lys Asn Val Pro
65 70 75 80
Cys Ser Arg Arg Cys Lys Glu Ile Glu Tyr Cys Asp Glu Gln Glu Lys
85 90 95
Ile Leu Glu Pro Asp Asp Pro Asp Gly Gly Trp Val Asp Thr His Arg
100 105 110
Val Asp Pro Thr Ser Gly Leu Asp Glu Asn Val Ser Gly Met Thr Leu
115 120 125
Asp Ser Asn Thr Thr Val Gln Arg Val Asn Asp Ser Pro Pro Ser Gly
130 135 140
Asp Phe Gln Thr Asn Thr Thr Asn Ala Asn Asn Asn Val Asp Asp Asp
145 150 155 160
Asp Asp Asp Asp Asp Asp Asn Glu Glu Ala Ala Asp Met Glu Met Phe
165 170 175
Glu Glu Ser Gly Leu Leu Asp Glu Glu Asp Glu Ala Thr Ala Glu Asp
180 185 190
Val Lys Ile Glu Lys Asp Glu Arg Asn Ala Ala Gly Asp Gly Glu Ile
195 200 205
Val Lys Thr Arg Thr Tyr Asp Leu His Ile Thr Tyr Asp Lys Tyr Tyr
210 215 220
Gln Thr Pro Arg Leu Trp Leu Phe Gly Tyr Asp Glu Asn His Lys Pro
225 230 235 240
Leu Asn Val Glu Gln Met Tyr Glu Asp Val Asn Gln Asp Tyr Ala Lys
245 250 255
Lys Thr Val Thr Met Glu Thr His Pro His Val Pro Gly Pro Pro Met
260 265 270
Ala Ser Val His Pro Cys Arg His Ala Glu Val Met Gln Lys Ile Ile
275 280 285
Gln Thr Val Leu Glu Gly Gly Gly Glu Leu Gly Val His Met Tyr Leu
290 295 300
Ile Ile Phe Leu Lys Phe Val Gln Ser Val Ile Pro Thr Ile Glu Tyr
305 310 315 320
Asp Tyr Thr Gln Asn Phe Thr Met Trp Ser Lys Thr Leu
325 330
Claims (5)
1. a kind of brown paddy plant hopperNlAtg3Gene, which is characterized in that for nucleotide sequence as shown in SEQ ID NO:1, which is brown
Plant hopper normal existence institute is required, and function, which is suppressed, will lead to the decline of brown paddy plant hopper survival rate.
2. a kind of brown paddy plant hopper as described in claim 1NlAtg3The albumen of gene coding, which is characterized in that amino acid sequence is such as
Shown in SEQ ID NO:2, which, which is suppressed, will lead to the decline of brown paddy plant hopper survival rate.
3. a kind of brown paddy plant hopper as described in claim 1NlAtg3The application of gene, which is characterized in that be used for agricultural chemicals research and development and life
Object prevents and treats brown paddy plant hopper.
4. one kind is directed to brown paddy plant hopper as described in claim 1NlAtg3The RNA perturbation technique of gene is in terms of controlling brown paddy plant hopper
Application, which is characterized in that the RNA perturbation technique causes the survival rate of brown paddy plant hopper to decline.
5. a kind of brown paddy plant hopper as claimed in claim 2NlAtg3The application of the albumen of gene coding, which is characterized in that be used for agriculture
Medicine research and development and biological control brown paddy plant hopper.
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| CN115925856A (en) * | 2022-07-19 | 2023-04-07 | 浙江大学 | Brown planthopper heat shock transcription factor NlHsf1, cDNA and application |
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| CN111019950A (en) * | 2019-11-18 | 2020-04-17 | 中国计量大学 | Nilaparvata lugens NlAtg1 gene, encoding protein and application thereof |
| CN112410346A (en) * | 2020-11-30 | 2021-02-26 | 中国计量大学 | Method for improving insect killing toxicity of biocontrol fungi |
| CN112410346B (en) * | 2020-11-30 | 2023-07-25 | 中国计量大学 | Method for improving biocontrol fungus insecticidal toxicity |
| CN115925856A (en) * | 2022-07-19 | 2023-04-07 | 浙江大学 | Brown planthopper heat shock transcription factor NlHsf1, cDNA and application |
| CN115925856B (en) * | 2022-07-19 | 2024-05-28 | 浙江大学 | Brown planthopper heat shock transcription factor NlHsf, cDNA and application |
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