CN109655588A - A kind of crops are to geological storage CO2Leak the integrated evaluating method of tolerance - Google Patents
A kind of crops are to geological storage CO2Leak the integrated evaluating method of tolerance Download PDFInfo
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Abstract
The invention discloses a kind of crops to geological storage CO2Leak the integrated evaluating method of tolerance, steps are as follows: the calculating of single index tolerance coefficient, according to related coefficient calculation method, correlation analysis is carried out to indices and calculates correlation matrix, based on correlation matrix, principal component analysis is carried out, original multiple indexs being relative to each other are converted into a few new overall target independent of each other, on the basis of principal component analysis, ∑ P is selectedjThe j principal component of > 0.85 calculates the CO of every kind of crop with following formula according to fuzzy synthetic appraisement method as overall target2Comprehensive tolerance index (Dk).The present invention is with the 8 kinds of typical case C in Loess Plateau of North Shaanxi area3、C4Crops are object, establish crop to CO2Tolerance evaluation method, screen to high concentration CO2Tolerant crops, provide reference frame for the Loess Plateau of North Shaanxi area Project Areas CCS Making A Strategic Decision of The Agricultural Productions.
Description
Technical field
The present invention relates to a kind of crops to CO2Tolerance evaluation method, specially a kind of crops are to geological storage CO2
Leak the integrated evaluating method of tolerance.
Background technique
Since CCS project construction is started late, the amount of sealing up for safekeeping is smaller, and there are no apparent CO occur2Leakage phenomenon, current
CO2The field to focus mostly in for CCS project is studied in influence of the leakage to plant or crop or indoor simulation is studied and other
CO2The field research of leakage point.Beaubien, Kr ü ger etc. are by natural CO2The leakage point hair of vegetative coverage research nearby
Existing, distance leakage center 6m range implants are impacted obvious, are just gradually recovered by the outside vegetation coverage in center；Pfanz H
Deng discovery volcano eruption later period CO2The raising of concentration can cause the photosynthetic decrease of timothy grass；Lakkaraju V R,
Amonette J E, Zhou X etc. utilize ZERT (Zero-Emission Research and Technology Centre) people
Work analog platform (USA) carries out simulation CO2Leakage experiment is observed in CO2The plant growth atrophy of source of leaks central area is even withered
Extremely；Pierce S, Patil R H etc. utilize ASGAD (Artificial Soil Gassing and Response
Detection) manual simulation's platform (England) carries out underground CO2It leaks the influence to soybean and herbage growth and studies discovery
Soybean growth is obviously inhibited, and biomass amount of decrease is larger；Wu Yang, field, Deng Hongzhang et al. (China) pass through indoor simulation
CO2Leakage different situations show that the decrement ratio corn of clover, wheat, pea root long and biomass is serious, and degree of susceptibility is more
Greatly.Many researchs are pointed out: different plants may be derived from the difference of different photosynthesis of plant approach to the difference of photosynthesis-carbon dioxide response
It is different.Although having some about CO2The case study influenced on plant/crop is leaked, but the understanding crop of system is to CO2Response
With qualitative assessment crop to CO2Tolerance it is still more rarely seen.Currently, plant is to CO2The correlative study of tolerance is less.Ma Xin
Et al. propose CO2There are the subjectivities of artificial setting target weight for comprehensive tolerance sex index (LCTI).
Summary of the invention
The purpose of the present invention is to provide a kind of crops to geological storage CO2The integrated evaluating method of tolerance is leaked,
The present invention establishes crop to CO2Tolerance evaluation method, screen to high concentration CO2Tolerant crops, for Loess Plateau of North Shaanxi
The area's Project Areas CCS Making A Strategic Decision of The Agricultural Productions provides reference frame, to solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme: a kind of crops are to geological storage CO2Leakage tolerance
The integrated evaluating method of property, specific steps include:
S1: the calculating of single index tolerance coefficient
The calculation formula of the tolerance coefficient of single index is as follows:
Refer to the average value under different disposal, VCKRefer to the measured value of control treatment；
S2: correlation analysis and principal component analysis
According to related coefficient calculation method, correlation analysis is carried out to indices and calculates correlation matrix, with phase
Based on relationship matrix number, carry out principal component analysis, by original multiple indexs being relative to each other be converted into new a few that
This independent overall target, formula are as follows:
Overall target ZjEquation:
ZjIndicate j-th of comprehensive index value, bijIndicate the feature vector of i-th of index in j-th of overall target, xiIt indicates
The tolerance coefficient of i-th of index；Calculate the characteristic value (λ of each principal componentj), contribution rate (Pj) and accumulation contribution rate (∑ Pj), lead to
Often the quantity for the principal component extracted is required to meet ∑ Pj> 0.85；
S3: the calculating of comprehensive tolerance index
On the basis of principal component analysis, ∑ P is selectedjThe j principal component of > 0.85 is comprehensive according to obscuring as overall target
Evaluation method is closed, the CO of every kind of crop is calculated with following formula2Comprehensive tolerance index (Dk):
Wherein:
Formula (4)In, indicate the membership function value of selected overall target；ZmaxIndicate j-th of synthesis of all crops
Index maximum value；ZminIndicate the minimum value of j-th of overall target of all crops, in formula (5), WjFor j-th of overall target
Weight.
Further, testing index is obtained by experiment, chooses 8 kinds of Typical crops, 4 kinds of C3Crop: mung bean, soya bean, buckwheat
Wheat, potato；4 kinds of C4Crop: corn, sorghum, millet, broom corn millet.
Further, experimental procedure is as follows:
Step 1: in an experiment, CO first2Growth cabinet is in obstructed CO2Under conditions of gas, by 8-10 basin, 4-5 plants/
Every kind of basin for selecting 6 basins to grow fine, 1-3 plants/basin crop, then in carbon dioxide when planting experimentally crop cultivation to the 2-3 leaf phase
Under controlled concentration, periodically watering, fixed fertilising are cultivated 30 days；
Step 2: after plantation, choosing plant height, the number of blade, longest root length, fresh weight, dry weight, Net Photosynthetic Rate
(Pn), transpiration rate (Tr), stomatal conductance (Gs), blade intercellular CO29 indexs of concentration (Ci) are as testing index.
Further, CO2CO in growth cabinet2Concentration is controlled respectively in 380 (CK), 10 000,20 000,40
000、80 000μmol·mol-1, experiment condition is set as 12h on daytime: 25 DEG C of temperature, humidity 80%, light intensity 100%；Night
12h: 20 DEG C of temperature, humidity 80%, light intensity 20%.
Further, Pn, Tr, Gs, Ci index are using the photosynthetic analyzer measurement of LI-6400X.
Compared with prior art, the beneficial effects of the present invention are:
Crops of the invention are to geological storage CO2The integrated evaluating method of tolerance is leaked, the present invention is according to CO2To plant
Object has the mechanism of fertilization effect and inhibiting effect double influence, and introduced plant is to CO2It is resistant to the concept of coefficient, with loess in North Shaanxi
8 kinds of highlands typical case C3、C4Crops are object, establish crop to CO2Tolerance evaluation method, screen to high concentration CO2
Tolerant crops, provide reference frame for the Loess Plateau of North Shaanxi area Project Areas CCS Making A Strategic Decision of The Agricultural Productions.
Specific embodiment
It will be detailed below the embodiment of the present invention, however, the embodiment of the present invention is not limited thereto.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts,
It shall fall within the protection scope of the present invention.
A kind of crops are to geological storage CO2The integrated evaluating method of tolerance is leaked, specific steps include:
Step 1: the calculating of single index tolerance coefficient
The calculation formula of the tolerance coefficient of single index is as follows:
Refer to the average value under different disposal, VCKRefer to the measured value of control treatment.
Step 2: correlation analysis and principal component analysis
According to related coefficient calculation method, correlation analysis is carried out to indices and calculates correlation matrix, with phase
Based on relationship matrix number, carry out principal component analysis, by original multiple indexs being relative to each other be converted into new a few that
This independent overall target, formula are as follows:
Overall target ZjEquation:
ZjIndicate j-th of comprehensive index value, bijIndicate the feature vector of i-th of index in j-th of overall target, xiIt indicates
The tolerance coefficient of i-th of index；Calculate the characteristic value (λ of each principal componentj), contribution rate (Pj) and accumulation contribution rate (∑ Pj), lead to
Often the quantity for the principal component extracted is required to meet ∑ Pj> 0.85.
Step 3: the calculating of comprehensive tolerance index
On the basis of principal component analysis, ∑ P is selectedjThe j principal component of > 0.85 is comprehensive according to obscuring as overall target
Evaluation method is closed, the CO of every kind of crop is calculated with following formula2Comprehensive tolerance index (Dk):
Wherein:
Formula (4)In, indicate the membership function value of selected overall target；ZmaxIndicate j-th of synthesis of all crops
Index maximum value；ZminIndicate the minimum value of j-th of overall target of all crops, in formula (5), WjFor j-th of overall target
Weight.
According to aforementioned crop to CO2The definition of tolerance, D value is bigger, and tolerance is stronger.
Testing index is obtained by experiment, chooses 8 kinds of Typical crops (including 4 kinds of C3Crop and 4 kinds of C4Crop), 4 kinds of C3Make
Object: mung bean, soya bean, buckwheat, potato；4 kinds of C4Crop: corn, sorghum, millet, broom corn millet.
Experimental procedure is as follows:
Step 1: in an experiment, CO first2Growth cabinet is in obstructed CO2Under conditions of gas, by 8-10 basin, 4-5 plants/
Every kind of basin for selecting 6 basins to grow fine, 1-3 plants/basin crop, then in carbon dioxide when planting experimentally crop cultivation to the 2-3 leaf phase
Under controlled concentration, periodically watering, fixed fertilising are cultivated 30 days；CO2CO in growth cabinet2Concentration is controlled respectively 380
(CK)、10 000、20 000、40 000、80 000μmol·mol-1, experiment condition is set as 12h on daytime: 25 DEG C of temperature, wet
Degree 80%, light intensity 100%；Night 12h: 20 DEG C of temperature, humidity 80%, light intensity 20%.
Step 2: choosing plant height, the number of blade, longest root length, fresh weight, dry weight, Net Photosynthetic Rate after plantation
(Pn), transpiration rate (Tr), stomatal conductance (Gs), blade intercellular CO29 indexs of concentration (Ci) are as testing index.Wherein,
Pn, Tr, Gs, Ci index are using the photosynthetic analyzer measurement of LI-6400X.
The laggard row crop of testing index is obtained to CO by testing above2Tolerance evaluation
1, single index is resistant to coefficient
It is resistant to coefficient formula (1) using single index, calculates every kind of crop single index CO2It is resistant to factor alpha (table 1).By
Table 1 is it is found that in 8 kinds of crops each single index tolerance coefficient, the tolerance of plant height, the number of blade, longest root length, fresh weight, dry weight
Coefficient is totally less than normal, intercellular CO2Concentration C i tolerance coefficient is obviously bigger than normal, and C3Pn, Tr, Gs index tolerance coefficient of crop are obvious
Less than C4Crop.
18 kinds of crop indices carbon dioxide of table are resistant to coefficient (xi)
2, correlation analysis and principal component analysis
Correlation analysis is carried out to each single index, single index correlation matrix (table 2) can be obtained.As shown in Table 2, it plants
Extremely significant correlation between each index of plant height degree, the number of blade, fresh weight, dry weight, Net Photosynthetic Rate, stomatal conductance；Longest root length
It is significant related to the number of blade, Net Photosynthetic Rate, transpiration rate；Intercellular CO2Concentration is uncorrelated to other indexs.
2 indices correlation matrix of table
Note: * * indicates extremely significant related (P < 0.01)；* significant related (P < 0.05) is indicated.
Principal component analysis is carried out to all indexs, obtains principal component characteristic value (λi), variance contribution ratio (Pj), cumulative variance
Accumulate contribution rate (∑ Pj) (table 3).As shown in Table 3, preceding 3 principal components accumulation variance contribution ratio can reach 92.24%.Therefore,
First three principal component is chosen as crop CO2Three overall targets of tolerance, three overall targets can reflect 9 single indexs
The linear relationship coefficient of 92.24% information, overall target and single index is shown in Table 4.
3 principal component characteristic value of table, variance contribution ratio and accumulation contribution rate
Linear relationship coefficient between 4 overall target of table and single index
3, tolerance overall merit
Each comprehensive index value Z of every kind of crop is calculated according to formula (2) and table 41、Z2And Z3Value calculates every according to formula (4)
Membership function value (the μ of kind crop comprehensive index value(Zj)), the weight of each overall target is calculated according to formula (5), it is last according to public
Formula (3) calculates the synthesis tolerance index (D) (table 5) of various crops.
The membership function of 58 kinds of crops of table and comprehensive tolerance index
According to aforementioned crop to CO2The definition of tolerance, D value is bigger, and tolerance is stronger.As shown in Table 5,8 kinds of crops
Synthesis tolerance index (D value) change the wherein C between 0.1459-0.86453Crop D value between 0.1459-0.4162 it
Between, C4Crop D value is between 0.8148-0.8645, i.e. C4Crop is to CO2Tolerance be apparently higher than C3Crop；8 kinds of experiments
Sex index is descending is followed successively by sorghum > broom corn millet > millet > corn > mung bean > soya bean > potato > buckwheat for crop tolerance,
I.e. in the common crop in Experimental Area, to CO2Strongest tolerance is C4Crop sorghum, it is most weak be C3Crop buckwheat.The present invention
Experiment conclusion be identical with practical potting simulated experiment crop Apparent character and biomass variety result.
It is resistant to law according to Shelford, biology has an ecological minimum and maximum to the adaptation of its living environment
Boundary, this minimum to maximum limit be known as biology tolerance range.Shelford answers the tolerance range of biology
Problem, but within tolerance range, there is also most suitable range and the difference of optimum range, ecological factor is resistant in optimum range
The measurement of degree becomes the object for needing to deepen again.The present invention is according to CO2Have fertilization effect and inhibiting effect dual plant
The mechanism of influence, introduced plant is to CO2It is resistant to the concept of coefficient, to express relative to normal atmosphere CO2High concentration when concentration
CO2Plant growth and development ability under stress.The growth and development ability of plant can be referred to physical signs, morphological index, biomass
The many indexes such as mark are measured, and just produce the difference of single index and overall target.Since there is one between single index
How fixed independence or complementarity, embody the comprehensive function of each single index, it is necessary to recognize and disappear by principal component analysis
Except the correlation of single index.
In summary: crops of the invention are to geological storage CO2Leak the integrated evaluating method of tolerance, root of the present invention
According to CO2There is the mechanism of fertilization effect and inhibiting effect double influence to plant, introduced plant is to CO2It is resistant to the concept of coefficient, with
The 8 kinds of typical case C in Loess Plateau of North Shaanxi area3、C4Crops are object, establish crop to CO2Tolerance evaluation method, screening pair
High concentration CO2Tolerant crops, provide reference frame for the Loess Plateau of North Shaanxi area Project Areas CCS Making A Strategic Decision of The Agricultural Productions.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of crops are to geological storage CO2Leak the integrated evaluating method of tolerance, which is characterized in that specific steps include:
S1: the calculating of single index tolerance coefficient
The calculation formula of the tolerance coefficient of single index is as follows:
Refer to the average value under different disposal, VCKRefer to the measured value of control treatment；
S2: correlation analysis and principal component analysis
According to related coefficient calculation method, correlation analysis is carried out to indices and calculates correlation matrix, with phase relation
Based on matrix number, principal component analysis is carried out, original multiple indexs being relative to each other are converted into new a few is only each other
Vertical overall target, formula are as follows:
Overall target ZjEquation:
ZjIndicate j-th of comprehensive index value, bijIndicate the feature vector of i-th of index in j-th of overall target, xiIt indicates i-th
The tolerance coefficient of index；Calculate the characteristic value (λ of each principal componentj), contribution rate (Pj) and accumulation contribution rate (∑ Pj).Usually want
The quantity of the principal component of extraction is asked to meet ∑ Pj> 0.85；
S3: the calculating of comprehensive tolerance index
On the basis of principal component analysis, ∑ P is selectedjThe j principal component of > 0.85 is commented as overall target according to fuzzy synthesis
Valence method calculates the CO of every kind of crop with following formula2Comprehensive tolerance index (Dk):
Wherein:
Formula (4)In, indicate the membership function value of selected overall target；ZmaxIndicate j-th of overall target of all crops
Maximum value；ZminIndicate the minimum value of j-th of overall target of all crops, in formula (5), WjFor the power of j-th of overall target
Weight.
2. a kind of crops according to claim 1 are to geological storage CO2The integrated evaluating method of tolerance is leaked, it is special
Sign is, obtains testing index by experiment, chooses 8 kinds of Typical crops, 4 kinds of C3Crop: mung bean, soya bean, buckwheat, potato；4
Kind C4Crop: corn, sorghum, millet, broom corn millet.
3. a kind of crops according to claim 2 are to geological storage CO2The integrated evaluating method of tolerance is leaked, it is special
Sign is that experimental procedure is as follows:
Step 1: in an experiment, CO first2Growth cabinet is in obstructed CO2It is under conditions of gas, 8-10 basin, 4-5 plants/basin is every
Then kind is controlled for selecting 6 basins to grow fine, 1-3 plants/basin crop when planting experimentally crop cultivation to the 2-3 leaf phase in carbon dioxide
Under concentration, periodically watering, fixed fertilising are cultivated 30 days；
Step 2: after plantation, choosing plant height, the number of blade, longest root length, fresh weight, dry weight, Net Photosynthetic Rate (Pn), steam
Rise rate (Tr), stomatal conductance (Gs), blade intercellular CO29 indexs of concentration (Ci) are as testing index.
4. a kind of crops according to claim 3 are to geological storage CO2The integrated evaluating method of tolerance is leaked, it is special
Sign is, CO2CO in growth cabinet2Concentration is controlled respectively in 380 (CK), 10 000,20 000,40 000,80 000 μ
mol·mol-1, experiment condition is set as 12h on daytime: 25 DEG C of temperature, humidity 80%, light intensity 100%；Night 12h: 20 DEG C of temperature,
Humidity 80%, light intensity 20%.
5. a kind of crops according to claim 3 are to geological storage CO2The integrated evaluating method of tolerance is leaked, it is special
Sign is that Pn, Tr, Gs, Ci index are using the photosynthetic analyzer measurement of LI-6400X.
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Cited By (1)
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| CN115577981A (en) * | 2022-11-22 | 2023-01-06 | 山东大学 | Screening method, system, equipment and storage medium for cadmium-resistant varieties of crops |
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Application publication date: 20190419 |