CN109655454A - A kind of method of different valence state iron ion content in measurement feed - Google Patents
A kind of method of different valence state iron ion content in measurement feed Download PDFInfo
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- CN109655454A CN109655454A CN201910079255.4A CN201910079255A CN109655454A CN 109655454 A CN109655454 A CN 109655454A CN 201910079255 A CN201910079255 A CN 201910079255A CN 109655454 A CN109655454 A CN 109655454A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention discloses a kind of methods of different valence state iron ion content in measurement feed, sodium thiosulfate is taken to shelter copper ion, the ferric ion in feed is reduced to ferrous ion with hydroxylamine hydrochloride, ferrous ion and luxuriant and rich with fragrance alloxazine are complexed to form the good violet complex of stability in pH=6 and under room temperature, reaction 30 minutes, it is detected at 562 nm by microplate reader, in conjunction with drafting standard curve, quickly, high throughput calculates the content of different valence state iron ion in feed, without complicated sample pre-treatments and expensive instrument, it is very suitable for promoting the use of in laboratories.
Description
Technical field
The present invention relates to feeding quality safety testing fields, more specifically, it relates to different price in a kind of measurement feed
The method of state iron ion content.
Background technique
Iron is that one of most wide, most common metal and the mankind, animal and plant growth are distributed on the earth is necessary micro
Element participates in the metabolic process of endotrophic substance.Iron deficiency easily leads to the diseases such as anaemia, and the iron of excess intake can improve tumour again
And cardiovascular and endocrine system disease risk.The content of iron can have an impact livestock birds health in feed, excessive Tie Tong mistake
Animal waste excretes, and can generate iron to plant and poison, to the growth of plant, chlorophyll content and activities of antioxidant enzymes etc.
It has an impact.Iron also will affect absorption of the plant to other metal ions.China provides that the limit standard of iron in feed is 250
Therefore mg/kg measures in feed iron content to ensureing that the mankind, animal and plant is healthy and safe has great importance.Meanwhile
The valence state of iron ion is affected to its absorption and utilization in animal body.Therefore, it is necessary to establish corresponding method to feeding
Iron ion total amount and valence state are measured in material.
The detection of iron mainly uses atomic absorption spectroscopy (AAS), inductively coupled plasma body-atomic emissions at present
The instrument analytical methods such as spectroscopic methodology (ICP-AES), inductively coupled plasma mass spectrometry analytic approach (ICP-MS), wherein atom is inhaled
Receive the national standard detection method that spectroscopic methodology is iron ion in the existing feed in China.Atomic absorption spectrography (AAS) have accuracy and
The features such as precision is high, but need expensive instrument and be equipped with professional operator, it is not suitable for laboratory, base.Simultaneously
Atomic absorption method sample pre-treatments clear up sample, need to consume strong acid, while digestion time is longer, are not suitable for scene
Quickly analysis.
In recent years, researcher also develops a variety of rapid analysis methods, such as electrochemical assay, spectrophotometry.Electricity
Chemical measure sensitivity with higher, but the preparation of electrode basement and modification are complex, and need to be equipped with electricity
Chem workstation.Spectrophotometry has the advantages that high sensitivity, instrument and equipment are simple, easy to operate, quick, but spectrophotometric
Iron is applied relatively broad at present in method measurement water quality, mainly there is rhodanate spectrophotometry, Phen spectrophotometry etc.,
But these detection method sensitivity are low, chromogenic reaction speed it is slow, be unsuitable for the measurement to iron ion in feed.Meanwhile it is conventional
Rapid analysis method cannot be measured the iron ion of different valence state.Therefore, a kind of aobvious based on micropore the present invention is directed to develop
Color method, and the laboratory method that high sensitivity, the range of linearity are wide, apparatus structure is simple and convenient to operate, for iron total in feed and
Quick, the high-throughput detection of different valence state iron ion.
Summary of the invention
In view of the deficiencies of the prior art, the present invention intends to provide a kind of measurement feed to solve the above problems
The method of middle different valence state iron ion content quick, high-throughput can detect different valence state iron ion content in feed,
Without complicated sample pre-treatments and expensive instrument, at low cost, strong antijamming capability, being highly suitable for laboratories popularization makes
With.
To achieve the above object, the present invention provides the following technical scheme that
The method of different valence state iron ion content, determination step are as follows in a kind of measurement feed:
(1) solution is prepared
A1 liquid: the luxuriant and rich with fragrance total iron working solution of alloxazine, including .4.0mM phenanthrene alloxazine solutions, 0.1M hydroxylamine hydrochloride solution and 0.1 M are thio
Metabisulfite solution, buffer are the acetum of ph=6；
A2 liquid: luxuriant and rich with fragrance alloxazine ferrous iron working solution, including 4.0 mM phenanthrene alloxazine solutions and 0.1 M hypo solution, buffer
For the acetum of ph=6；
B liquid: blank solution, 0.2M hac buffer；
C liquid: 0,0.1,0.5,1,2,4,6,8 μ g/mL of iron standard solution, respectively C1-C8 iron titer；
(2) sample pre-treatments
Feed Sample is mixed with 1% solution of trichloroacetic acid after crushed, ratio 1:100(g/ml), by quantitatively filtering after oscillation
Paper filtering or centrifugation obtain supernatant；
(3) it detects
Standard curve group: the C1-C8 iron titer of various concentration is added in every hole in ELISA Plate hole, and then the A1 of equivalent is added in every hole
Liquid；
Sample sets: the measurement of each sample needs two groups of ELISA Plate holes, and one group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A1 liquid, separately
One group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A2 liquid；
Sample sets and standard curve group are subjected to color contrast, observes by the naked eye and half-quantitative detection is carried out to iron concentration.
The method of different valence state iron ion content, determination step are as follows in a kind of measurement feed:
(1) solution is prepared
A1 liquid: the luxuriant and rich with fragrance total iron working solution of alloxazine, including .4.0mM phenanthrene alloxazine solutions, 0.1M hydroxylamine hydrochloride solution and 0.1 M are thio
Metabisulfite solution, buffer are the acetum of ph=6；
A2 liquid: luxuriant and rich with fragrance alloxazine ferrous iron working solution, including 4.0 mM phenanthrene alloxazine solutions and 0.1 M hypo solution, buffer
For the acetum of ph=6；
B liquid: blank solution, 0.2M hac buffer；
C liquid: 0,0.1,0.5,1,2,4,6,8 μ g/mL of iron standard solution, respectively C1-C8 iron titer；
(2) sample pre-treatments
Feed Sample is mixed with 1% solution of trichloroacetic acid after crushed, ratio 1:100(g/ml), by quantitatively filtering after oscillation
Paper filtering or centrifugation obtain supernatant；
(3) it detects
Standard curve group: the C1-C8 iron titer of various concentration is added in every hole in ELISA Plate hole, and then the A1 of equivalent is added in every hole
Liquid；
Sample sets: the measurement of each sample needs two groups of ELISA Plate holes, and one group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A1 liquid, separately
One group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A2 liquid；
After all measurement holes are reacted 30 minutes at 20-25 DEG C of room temperature after sample-adding, suction is read at 562 nm through microplate reader
Shading value；
(4) concentration calculation
To absorbance value AcWith concentration of standard solution CM-StdLinear fit is carried out, the slope of standard curve is obtained according to following equation
βM:
Total concentration of iron in sample extracting solution is calculated by following publicity:
CM-Sample = (ASample-b)/βM×N
In formula, ASampleFor sample extracting solution detection hole light absorption value, b is the intercept of standard curve, and N is feed extract dilution times
Number；Calculate: it is total concentration of iron that A1 liquid sample well, which is added, and calculates gained, and it is ferrous ion that A2 liquid sample well, which is added, and calculates gained
Content, the difference of the two are ferric ion content.
It is to mix Feed Sample with the solution of trichloroacetic acid of 1%-4% after crushed that the present invention, which is further improved technical solution,
Close, ratio 1:100(g/ml), it shakes 5 minutes, ultrasonic extraction 15-30 minutes, is filtered by quantitative filter paper or centrifugation obtains
Clear liquid is measured.
The further improved technology scheme of the present invention is that each sample measurement needs 4 ELISA Plate detection holes, and two are one group
In parallel.
Luxuriant and rich with fragrance alloxazine be it is a kind of can be with the color developing agent that effectively be complexed of ferrous iron, its high sensitivity, chromogenic reaction is fast, with ferrous iron from
The Fe-Ferrozine Stability of Metal Complexes that son is formed is good, but luxuriant and rich with fragrance alloxazine can also react simultaneously with copper ion, to iron ion
Measurement generate interference.The present invention takes sodium thiosulfate to shelter copper ion.Ferric ion hydrochloric acid in feed
Hydroxylamine reduction is ferrous ion, and ferrous ion is complexed to form violet complex in pH=6, under room temperature with luxuriant and rich with fragrance alloxazine, instead
After answering 30 minutes, absorbance value is read at 562 nm by microplate reader, by drawing standard curve, is calculated different in feed
The content of valence state iron.The present invention passes through the standard curve that system optimization reaction condition, luxuriant and rich with fragrance alloxazine and ferrous iron and ferric iron generate
Substantially it coincide, therefore, this method, which need to only establish a set of standard curve, can measure two kinds of valence state iron ions.
The invention has the advantages that:
One, the present invention is highly suitable for laboratories popularization and use without complicated sample pre-treatments and expensive instrument.
Two, the present invention can be used for the detection of Feed Sample iron ion, and strong antijamming capability, detection limit is low, substantially eliminate it
His interference of the ingredient to detection.
Three, the present invention both can carry out quantitative detection with microplate reader, can also detect by an unaided eye, what standard of comparison solution generated
Color carries out half-quantitative detection to iron concentration.
Four, the present invention can disposably detect batch samples using ELISA Plate as carrier.
Five, the present invention makes full use of color developing agent and different valence state iron response characteristic, can detect different price in feed simultaneously
State iron content.
Detailed description of the invention
Fig. 1 is canonical plotting；
Fig. 2 is the different absorbing wavelength curve graphs of various concentration iron ion；
Fig. 3 is the Fe-Ferrozine complex compound light absorption value influence situation that different PH forms iron ion standard solution, wherein
(A) figure is that pH value influences situation to the Fe-Ferrozine complex compound light absorption value that ferric ion standard solution is formed；(B) figure is
PH value influences situation to the Fe-Ferrozine complex compound light absorption value that ferrous ion standard solution is formed；
Fig. 4 reacts the absorbance change situation in 10 h with luxuriant and rich with fragrance alloxazine for various concentration iron ion under condition of different temperatures；
The case where Fig. 5 is absorbance change of iron ion under the conditions of different luxuriant and rich with fragrance alloxazine concentrations；
Fig. 6 is the disturbed condition of other ion pair phenanthrene alloxazine reaction detection copper before sheltering, wherein (A) figure is the preceding 4 μ g/mL of masking
The disturbed condition of each metal ion and luxuriant and rich with fragrance alloxazine, (B) figure are the interference feelings of 4 each metal ions of μ g/mL and luxuriant and rich with fragrance alloxazine after masking
Condition.
Specific embodiment
Embodiment 1: different valence state iron ion detection scheme in feed
(1) solution is prepared
A1 liquid: the luxuriant and rich with fragrance total iron working solution of alloxazine, including .4.0mM phenanthrene alloxazine solutions, 0.1M hydroxylamine hydrochloride solution and 0.1 M are thio
Metabisulfite solution, buffer are the acetum of ph=6；
A2 liquid: luxuriant and rich with fragrance alloxazine ferrous iron working solution, including 4.0 mM phenanthrene alloxazine solutions and 0.1 M hypo solution, buffer
For the acetum of ph=6；
B liquid: blank solution, 0.2M hac buffer；
C liquid: 0,0.1,0.5,1,2,4,6,8 μ g/mL of iron standard solution, respectively C1-C8 iron titer；
(2) sample pre-treatments
Feed Sample is mixed with 1% solution of trichloroacetic acid after crushed, ratio 1:100(g/ml), by quantitatively filtering after oscillation
Paper filtering or centrifugation obtain supernatant；
(3) it detects
Standard curve group: the C1-C8 iron titer of various concentration is added in every hole in ELISA Plate hole, and then the A1 of equivalent is added in every hole
Liquid；
Sample sets: the measurement of each sample needs two groups of ELISA Plate holes, and one group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A1 liquid, separately
One group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A2 liquid；
After all measurement holes are reacted 30 minutes at 20-25 DEG C of room temperature after sample-adding, suction is read at 562 nm through microplate reader
Shading value；
(4) concentration calculation
To absorbance value AcWith concentration of standard solution CM-StdLinear fit is carried out, the slope of standard curve is obtained according to following equation
βM:
Total concentration of iron in sample extracting solution is calculated by following publicity:
CM-Sample = (ASample-b)/βM×N
In formula, ASampleFor sample extracting solution detection hole light absorption value, b is the intercept of standard curve, and N is feed extract dilution times
Number；Calculate: it is total concentration of iron that A1 liquid sample well, which is added, and calculates gained, and it is ferrous ion that A2 liquid sample well, which is added, and calculates gained
Content, the difference of the two are ferric ion content.
Embodiment 2: the drafting of working curve
Take the Fe of 0,0.1,0.5,1,2,4,8 μ g/mL3+100 μ L of standard solution is added in 96 orifice plates, adds total iron working solution
Absorbance measurement is carried out at 562 nm with microplate reader after 100 μ L, 30 min.Figure l is 5 measurement Fe3+Standard series average value
Regression curve.Find out that correlation coefficient r is 0.9999 from result, can measure concentration range is 0.1-8 μ g/L.The content of iron exists
In a linear relationship within the scope of 0.1-8 μ g/L, regression equation is y=0.1425x+0.0537.
Embodiment 3: the selection of Detection wavelength
As shown in Fig. 2, a length of 562 nm of maximum absorption wave of Fe- phenanthrene alloxazine complex compound, therefore, to improve detection sensitivity and drop
Low interference, the optimal wavelength that we select 562 nm to measure as iron ion.
Embodiment 4: the optimization of determination condition
1, the selection of pH value in reaction
First by adjusting the pH value of reaction system, the influence that different pH value form Fe- phenanthrene alloxazine complex compound is had studied, sees figure
3, wherein figure a is the influence for the Fe-Ferrozine complex compound light absorption value that pH value forms ferric ion standard solution, scheme b
For the influence for the Fe-Ferrozine complex compound light absorption value that pH value forms ferrous ion standard solution.The result shows that working as pH
Value within the scope of 4-11, the complex compound light absorption value of formation without significant difference, but in view of in iron working solution sodium thiosulfate it is steady
It is qualitative, it avoids sodium thiosulfate from decomposing in acid condition and generates sulphur precipitating, influence shading value.Additionally due in different water samples
The difference of acid, basicity, for the consistency for ensuring different example reaction conditions, therefore the final sodium acetate using high concentration of this experiment
Buffer (0.2M, pH=6) is as reaction buffer system.
2, the selection of reaction temperature and time
The present invention has studied the influence that reaction time and temperature form Fe-Ferrozine complex compound, and Fig. 4 is various concentration
(1,2,4,6,8 μ g/mL) iron ion reacts the absorbance change situation in 10 h with luxuriant and rich with fragrance alloxazine under condition of different temperatures.Knot
Fruit shows Fe- phenanthrene alloxazine complex compound synthesis speed quickly, and smaller by reaction temperatures affect, and it is steady to form purple in 30 minutes
Determine complex compound, and Fe- phenanthrene alloxazine complex compound can stablize 10 h at 4 DEG C, 25 DEG C, 37 DEG C.Since object of this investigation is exploitation
A kind of in-situ check and test method, preferred condition are to be reacted under room temperature.Comprehensive analysis, this test have determined room temperature (20-
25 DEG C) it is peak optimization reaction temperature.According to Fig. 4's as a result, the absorbance value of reaction 30 minutes is without significant difference at room temperature, therefore
It was determined as optimum reacting time for 30 minutes.
3, the selection of chromogenic agent
This experiment passes through the influence that the concentration studies for adjusting iron working solution China and Philippines alloxazine chromogenic agent detects iron.Fig. 5 is
The case where absorbance change of iron ion under the conditions of different luxuriant and rich with fragrance alloxazine concentration (0.05%, 0.1%, 0.2%, 1%).Fig. 5 shows, with
The increase of iron working solution China and Philippines alloxazine concentration, the concentration of iron range of linearity of measurement also increases, when concentration increases to 0.2%, iron
The measurement range of linearity remains unchanged.Because the detection range of this experiment is 0-8 μ g/mL, select 0.2% luxuriant and rich with fragrance alloxazine dense
Degree.
4, the interference and masking of other ions
Since there is a lot of other metal ions in feed, the detection of iron ion is very likely by the interference of other ions.
Therefore, the ion that this experiment is higher to content in feed, may generate interference to Fe and Ti has carried out cross reaction experimental analysis.
The light absorption value that the iron ion standard solution of 4 μ g/mL measures is set as background value by this experiment, is divided into iron ion standard solution
Not Tian Jia concentration be 1-1000 μ g/mL Cu2+、Zn2+、Mg2+、Mn2+、Ca2+, add iron working solution measurement light absorption value.According to
Following equation calculates interference percentage (I.P.), with the disturbed condition of the other ions of quantificational expression.
The result of cross reaction analysis is as shown in fig. 6, work as Mg2+、Mn2+、Ca2+And Zn2+Concentration respectively reaches 1000 μ g/ of >
1000 μ g/mL of mL, >, 1000 > μ g/mL and 500 μ g/mL, interference percentage reach 5%.It can thus be assumed that in Feed Sample
The Zn coexisted2+、Mg2+、Mn2+、Ca2+The detection of iron will not be made a significant impact.And works as and be added to greater than 20 μ g/mL Cu2+
Afterwards, interference percentage reaches 5%.Show Cu2+Larger interference can be generated to the measurement of iron, this is consistent with document report, it is therefore desirable to
Interfering ion is sheltered using screening agent.By optimization, this experimental selection sodium thiosulfate shelters copper ion.Through
Repetition test determines that the hypo solution that 0.1M is added in working solution is used for the masking of copper ion.On this basis, it measures
The cross reaction situation of iron ion measurement after masking copper ion.The result shows that sodium thiosulfate has well copper ion
Masking action.Therefore, the masking agent prescription of this optimum experimental can effectively eliminate coexisting ion in feed and measure to iron ion
Interference.Fig. 6 is the disturbed condition of (A) before sheltering, rear (B) other ion pair phenanthrene alloxazine reaction detection copper, wherein before (A) masking
The disturbed condition of 4 each metal ions of μ g/mL and luxuriant and rich with fragrance alloxazine, the interference of 4 each metal ions of μ g/mL and luxuriant and rich with fragrance alloxazine after (B) masking
Situation.
Embodiment 5: addition recovery test
Swine feed and chicken mixed feed are taken, using the content of iron ion background values in aas determination each sample.With
The iron ion standard solution of high concentration is added into each sample afterwards, until it is 0,20,100,200 mg/kg that concentration is added in feed,
Each concentration 6 parallel.It is measured using the method for the present invention.Measured value is calculated into the rate of recovery divided by add value, while calculating 6
The relative standard deviation of a parallel determination.As a result shown in following table.The result shows that: in the concentration range of addition, average recovery rate
Between 89.65 %-107.50 %, relative standard deviation meets the requirement of quantitative analysis less than 15%.It see the table below 1.
Table 1
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-described embodiment,
All technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
For those of ordinary skill, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (4)
1. a kind of method of different valence state iron ion content in measurement feed, which is characterized in that its determination step is as follows:
(1) solution is prepared
A1Liquid: the luxuriant and rich with fragrance total iron working solution of alloxazine, including 4.0 mM phenanthrene alloxazine solutions, 0.1M hydroxylamine hydrochloride be molten and 0.1 M thiosulfuric acid
Sodium solution, buffer are the acetum of pH=6；
A2Liquid: luxuriant and rich with fragrance alloxazine ferrous iron working solution, including 4.0 mM phenanthrene alloxazine solutions and 0.1 M hypo solution, buffer be
The acetum of ph=6；
B liquid: blank solution, 0.2M hac buffer；
C liquid: 0,0.1,0.5,1,2,4,6,8 μ g/mL of iron standard solution, respectively C1-C8 iron titer；
(2) sample pre-treatments
Feed Sample is mixed with the solution of trichloroacetic acid of 1%-4% after crushed, ratio 1:100(g/ml), it shakes 5 minutes,
It ultrasonic extraction 15-30 minutes, is filtered by quantitative filter paper or centrifugation obtains supernatant and is measured；
(3) it detects
Standard curve group: the C1-C8 iron titer of various concentration is added in every hole in ELISA Plate hole, and then the A1 of equivalent is added in every hole
Liquid；
Sample sets: the measurement of each sample needs two groups of ELISA Plate holes, and one group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A1 liquid, separately
One group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A2 liquid；
Sample sets are carried out color with standard curve group to compare, half-quantitative detection is carried out to iron concentration.
2. a kind of method of different valence state iron ion content in measurement feed, which is characterized in that its determination step is as follows:
(1) solution is prepared
A1Liquid: the luxuriant and rich with fragrance total iron working solution of alloxazine, including .4.0mM phenanthrene alloxazine solutions, 0.1M hydroxylamine hydrochloride solution and the thio sulphur of 0.1 M
Acid sodium solution, buffer are the acetum of ph=6；
A2Liquid: luxuriant and rich with fragrance alloxazine ferrous iron working solution, including 4.0 mM phenanthrene alloxazine solutions and 0.1 M hypo solution, buffer be
The acetum of ph=6；
B liquid: blank solution, 0.2M hac buffer；
C liquid: 0,0.1,0.5,1,2,4,6,8 μ g/mL of iron standard solution, respectively C1-C8 iron titer；
(2) sample pre-treatments
Feed Sample is mixed with the solution of trichloroacetic acid of 1%-4% after crushed, ratio 1:100(g/ml), it shakes 5 minutes,
It ultrasonic extraction 15-30 minutes, is filtered by quantitative filter paper or centrifugation obtains supernatant and is measured；
(3) it detects
Standard curve group: the C1-C8 iron titer of various concentration is added in every hole in ELISA Plate hole, and then the A1 of equivalent is added in every hole
Liquid；
Sample sets: the measurement of each sample needs two groups of ELISA Plate holes, and one group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A1 liquid, separately
One group of measurement Kong Zhongxian adds sample liquid, then plus equivalent A2 liquid；
After all measurement holes are reacted 30 minutes at 20-25 DEG C of room temperature after being loaded, read at 562 nm through microplate reader
Absorbance value；
(4) concentration calculation
To absorbance value AcWith concentration of standard solution CM-StdLinear fit is carried out, the slope of standard curve is obtained according to following equation
βM:
Total concentration of iron in sample extracting solution is calculated by following publicity:
CM-Sample = (ASample-b)/βM×N
In formula, ASampleFor sample extracting solution detection hole light absorption value, b is the intercept of standard curve, and N is feed extract dilution times
Number；
Calculate: be added A1 liquid sample well calculate gained be total concentration of iron, be added A2 liquid sample well calculate gained be ferrous iron from
Sub- content, the difference of the two are ferric ion content.
3. the method for different valence state iron ion content, feature exist in a kind of measurement feed according to claim 1 or 2
In: Feed Sample is mixed with the solution of trichloroacetic acid of 1%-4% after crushed, ratio 1:100(g/ml), it shakes 5 minutes, surpasses
Sound extracts 15-30 minutes, is filtered by quantitative filter paper or centrifugation obtains supernatant and is measured.
4. the method for different valence state iron ion content, feature exist in a kind of measurement feed according to claim 1 or 2
In: each sample measurement needs 4 ELISA Plate detection holes, and two are parallel for one group.
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| CN111896418A (en) * | 2020-06-29 | 2020-11-06 | 广东韶钢松山股份有限公司 | Method for measuring sulfur content in ferrosulfur alloy |
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| CN111751355A (en) * | 2020-07-22 | 2020-10-09 | 攀钢集团研究院有限公司 | Method for determining content of ferrous iron ions in Fe (II) -EDTA (ethylene diamine tetraacetic acid) complex denitration liquid |
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| CN113607878A (en) * | 2021-07-29 | 2021-11-05 | 北京首钢股份有限公司 | Assay method for concentration of free acid and ferrous ion in pickling waste hydrochloric acid and application |
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