CN109646139A - A kind of calf arterial blood drawing method - Google Patents
A kind of calf arterial blood drawing method Download PDFInfo
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- CN109646139A CN109646139A CN201910017493.2A CN201910017493A CN109646139A CN 109646139 A CN109646139 A CN 109646139A CN 201910017493 A CN201910017493 A CN 201910017493A CN 109646139 A CN109646139 A CN 109646139A
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- blood
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- drawing method
- silicone tube
- blood drawing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
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- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of calf arterial blood drawing method, including the following steps: 1) collection blood bucket with purified water clean 2-3 time, then uses 75% medicinal alcohol immersion 30-50min, it is spare to be placed on clean area after drying；2) by the medical silicone tube after the soaking with sodium hydroxide with 2% and blood tool is connect, is rinsed 2-5 time with purified water, blood drawing tool and both ends are then sealed into the heparin tube after wrapping up with masking foil are put into high-pressure sterilizing pot and sterilizes, it is spare to stay；3) calf is placed into operating table, conventional preserved skin will be carried out at calf neck arteries, is then carried out disinfection with 5% bromogeramine solution；4) calf neck is cut open after sterilizing, finds out arteries, cuts open osculum, is inserted into medical silicone tube, the other end of medical silicone tube is placed in collection blood bucket entrance, and blood was collected.Blood-sampling method of the invention can quickly take a blood sample, and blood sampling volume is big, and blood-sample withdrawal is not subject to pollute, and be suitable for that large-scale promotion uses.
Description
Technical field
The present invention relates to medic laboratory technology fields, and in particular to a kind of calf arterial blood drawing method.
Background technique
The production and preparation of animal antiserum, generally firstly the need of to animal carry out passed examination after can bloodletting.At present
The method of blood sampling bloodletting mainly includes eye socket blood sampling, capillary blood sampling, Culling heart blood, broken end blood sampling etc..Wherein, eye socket
Operator's left hand thumb and index finger gently oppress neck two sides when blood sampling is generally taken a blood sample, and keep orbital venous plexus congested, then adopt
Enter blood sampling after being pierced into blood taking needle；Capillary blood sampling is usually to carry out when needing blood volume few in animal finger or heel
Blood sampling；Culling heart blood, general requirement is stringenter, if entering heart, the depth of inserting needle and the internal diameter of syringe needle through body puncture
Size can all influence the speed and quantity of blood sampling, and the too long easy blood coagulation of blood sampling time, influence taken a blood sample quality, and split
Blood was collected for animal shirtfront, and animal can be dead rapidly, this can seriously affect blood sampling volume；Neck blood sampling is smeared as the term suggests being that will directly move
The neck of object is cut off, and then blood was collected, and this blood-sampling method is generally easy to pollute blood-sample withdrawal by tissue fluid, and then is influenced
Subsequent production, and blood-sampling method is more cruel bloody, is not suitable for being accepted by the public.
Summary of the invention
In view of the above-mentioned problems, the blood-sampling method can be quickly the object of the present invention is to provide a kind of calf arterial blood drawing method
Blood sampling, and blood sampling volume is big, blood-sample withdrawal is not subject to pollute, and is suitable for that large-scale promotion uses.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of calf arterial blood drawing method, including the following steps: 1) collection blood bucket with purified water is cleaned 2-3 time, is then made
30-50min is impregnated with 75% medicinal alcohol, it is spare to be placed on clean area after drying；It 2) will be after the soaking with sodium hydroxide with 2%
Medical silicone tube and blood tool is connect, rinse 2-5 time with purified water, blood drawing tool and both ends with masking foil are then sealed into wrapping
Heparin tube afterwards is put into sterilizing in high-pressure sterilizing pot, spare to stay；3) calf is placed into operating table, by calf neck arteries into
Row routine preserved skin, is then carried out disinfection with 5% bromogeramine solution；4) calf neck is cut open after sterilizing, finds out arteries,
Osculum is cut open, medical silicone tube is inserted into, the other end of medical silicone tube is placed in collection blood bucket entrance, and blood was collected.
It is ligatured, is then reserved with for being inserted into medical silicon using operation silk thread at the osculum cut open in the step 4)
The port of sebific duct.
Medical silicone tube used in the step 4) includes hose, and an end of hose blood sampling is connected with blood sampling pipette tips,
The end face for pipette tips of taking a blood sample is equipped with groove on the outside of the hose of pipette tips of taking a blood sample and hose joint, is adopted in step 4) in conical-surface type
When blood, after being inserted into medical silicone tube, groove be in the position that operation silk thread ligatures, and when blood sampling tenses operation silk thread, operation silk thread
In groove.
Hemostatic clamp is additionally provided on the hose.
The hose is polyethylene material, inner diameter 0.1-0.4mm.
Water in the step 1) and step 2 is purified water.
Sterilising temp in the step 2 is 121 DEG C, sterilization time 15min.
Disinfection is carried out disinfection using 5% bromogeramine solution in the step 3).
Sterilizer model MJQ70-I type or LDZX-50KBS model in the step 2.
Compared with prior art, the present invention the present invention is to calf neck arteries, blood was collected, only need to cut open small mouth i.e.
It can largely take a blood sample, blood sampling efficiency is relatively high, and can make that blood coagulation phenomenon will not occur in the blood sample short time adopted, different to meet
Detection needs, and in addition directly to neck arteries, blood was collected using hose, guarantee during blood sampling will not by vivo other
The pollution of tissue fluid guarantees the clean of blood, improves the accuracy of entire detection test.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of medical silicone tube in the present invention.
Specific embodiment
To more fully understand those skilled in the art to the present invention, the present invention is done With reference to embodiment
It further illustrates:
Calf arterial blood drawing method provided by the invention, including the following steps: 1) blood bucket will be collected and clean 2-3 with purified water
It is secondary, 30-50min then is impregnated using 75% medicinal alcohol, it is spare to be placed on clean area after drying；It 2) will be with 2% sodium hydroxide
Medical silicone tube after immersion and blood tool is connect, is rinsed 2-5 times with purified water, then by blood drawing tool and both ends masking foil
Heparin tube after sealing wrapping is put into sterilizing in high-pressure sterilizing pot, spare to stay；3) calf is placed into operating table, by calf neck
Conventional preserved skin is carried out at artery, is then carried out disinfection with 5% bromogeramine solution；4) calf neck is cut open after sterilizing, is found out dynamic
Arteries and veins blood vessel cuts open osculum, is inserted into medical silicone tube, and the other end of medical silicone tube is placed in collection blood bucket entrance, and blood was collected.
It is ligatured, is then reserved with for being inserted into medical silicone tube using operation silk thread at the osculum cut open in step 4)
Port, operation silk thread can tighten medical silicone tube when guaranteeing blood sampling, guarantee blood in addition to medical silicone tube will not outflow,
Guarantee blood sampling volume and blood sampling efficiency, blood clotting phenomenon occurs to avoid blood sampling time length.
As shown in Figure 1, medical silicone tube used in step 4) includes hose 1, the end that hose 1 is taken a blood sample, which is connected with, is adopted
The end face of blood pipette tips 3, blood sampling pipette tips 3 is in conical-surface type, in order to be rapidly inserted into the osculum that arteries is cut open, pipette tips of taking a blood sample
3 and 1 joint of hose hose on the outside of be equipped with groove 2, when blood was collected for step 4), be inserted into medical silicone tube after, at groove 2
Operation silk thread is tensed when the position that operation silk thread ligatures, blood sampling, operation silk thread is in groove, and being more advantageous to operation silk thread will
Hose tightens, to guarantee that blood will not flow out on the outside of medical silicone tube, that is, the health guaranteed, while blood sampling efficiency is also improved,
Guarantee biggish blood sampling volume.
It is additionally provided with hemostatic clamp on the hose, prevents from falling off to lead to be fixed on hose at the arteria carotis osculum of blood sampling
Blood sample is caused to be polluted.
Used hose is polyethylene material, and according to actual needs, 0.1mm, 0.2mm, 0.3mm can be used in inner diameter
Or 0.4mm.
Water in the step 1) and step 2 is purified water, to guarantee the safety of various utensils used in blood collection procedure
Health avoids other pollutants in common water quality from remaining on utensil and pollutes blood sample and then influence subsequent production use.
Sterilising temp in the step 2 is 121 DEG C, and sterilization time 15min, preferably 15min sterilize at this temperature
Not only it can guarantee within 15 minutes the effect of sterilizing, but also the too many time will not be wasted in preliminary preparation, and improved whole blood sampling efficiency.
Disinfection is carried out disinfection using 5% bromogeramine solution in step 3), clean at the neck arteries to guarantee blood sampling
Health avoids blood-sample withdrawal from being polluted.
According to actual needs, the high-pressure sterilizing pot in step 2 is using MJQ70-I model or LDZX-50KBS model
Autoclave.
Claims (9)
1. a kind of calf arterial blood drawing method, it is characterised in that: including the following steps: 1) collection blood bucket is washed with water into 2-3
It is secondary, 30-50min then is impregnated using 75% medicinal alcohol, it is spare to be placed on clean area after drying；It 2) will be with 2% sodium hydroxide
Medical silicone tube after immersion and blood tool is connect, is rinsed with water 2-5 times, then seals blood drawing tool and both ends with masking foil
Heparin tube after wrapping is put into sterilizing in high-pressure sterilizing pot, spare to stay；3) calf is placed into operating table, by calf neck arteries
Place carries out conventional preserved skin, is then carried out disinfection with bromogeramine solution；4) calf neck is cut open after sterilizing, finds out arteries,
Osculum is cut open, medical silicone tube is inserted into, the other end of medical silicone tube is placed in collection blood bucket entrance, and blood was collected.
2. calf arterial blood drawing method according to claim 1, it is characterised in that: in the osculum cut open in the step 4)
Place is ligatured using operation silk thread, is then reserved with the port for being inserted into medical silicone tube.
3. calf arterial blood drawing method according to claim 2, it is characterised in that: medical silicon used in the step 4)
Sebific duct includes hose, and an end of hose blood sampling is connected with blood sampling pipette tips, and the end face for pipette tips of taking a blood sample is in conical-surface type, blood sampling pipette tips and
Groove is equipped on the outside of the hose of hose joint, when blood was collected for step 4), after being inserted into medical silicone tube, groove is in operation
The position of silk thread ligation, when blood sampling, tense operation silk thread, and operation silk thread is in groove.
4. calf arterial blood drawing method according to claim 3, it is characterised in that: be additionally provided with hemostatic clamp on the hose.
5. calf arterial blood drawing method according to claim 3, it is characterised in that: the hose is polyethylene material, soft
Bore is 0.1-0.4mm.
6. calf arterial blood drawing method according to claim 1, it is characterised in that: the water in the step 1) and step 2
For purified water.
7. calf arterial blood drawing method according to claim 1, it is characterised in that: the sterilising temp in the step 2 is
121 DEG C, sterilization time 15min.
8. calf arterial blood drawing method according to claim 1, it is characterised in that: disinfection is using 5% in the step 3)
Bromogeramine solution carries out disinfection.
9. calf arterial blood drawing method according to claim 1, it is characterised in that: the high-pressure sterilizing pot in the step 2
Model MJQ70-I type or LDZX-50KBS model.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910017493.2A CN109646139A (en) | 2019-01-09 | 2019-01-09 | A kind of calf arterial blood drawing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910017493.2A CN109646139A (en) | 2019-01-09 | 2019-01-09 | A kind of calf arterial blood drawing method |
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| Publication Number | Publication Date |
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| CN109646139A true CN109646139A (en) | 2019-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910017493.2A Pending CN109646139A (en) | 2019-01-09 | 2019-01-09 | A kind of calf arterial blood drawing method |
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| CN (1) | CN109646139A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112674902A (en) * | 2021-01-18 | 2021-04-20 | 皖南医学院 | Experimental animal artery cannula and experimental method thereof |
-
2019
- 2019-01-09 CN CN201910017493.2A patent/CN109646139A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112674902A (en) * | 2021-01-18 | 2021-04-20 | 皖南医学院 | Experimental animal artery cannula and experimental method thereof |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190419 |
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| RJ01 | Rejection of invention patent application after publication |