CN109645936A - A kind of burnt based endoscopic imaging alignment correction system and method for copolymerization - Google Patents
A kind of burnt based endoscopic imaging alignment correction system and method for copolymerization Download PDFInfo
- Publication number
- CN109645936A CN109645936A CN201811583549.2A CN201811583549A CN109645936A CN 109645936 A CN109645936 A CN 109645936A CN 201811583549 A CN201811583549 A CN 201811583549A CN 109645936 A CN109645936 A CN 109645936A
- Authority
- CN
- China
- Prior art keywords
- image
- scanning galvanometer
- axis scanning
- galvanometer
- pixel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003384 imaging method Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000012937 correction Methods 0.000 title claims abstract description 14
- 238000007334 copolymerization reaction Methods 0.000 title claims abstract description 9
- 230000003287 optical effect Effects 0.000 claims abstract description 23
- 230000008878 coupling Effects 0.000 claims abstract description 15
- 238000010168 coupling process Methods 0.000 claims abstract description 15
- 238000005859 coupling reaction Methods 0.000 claims abstract description 15
- 238000005070 sampling Methods 0.000 claims abstract description 15
- 239000000835 fiber Substances 0.000 claims abstract description 14
- 238000005314 correlation function Methods 0.000 claims abstract description 6
- 239000011159 matrix material Substances 0.000 claims description 9
- 230000000875 corresponding effect Effects 0.000 claims description 6
- 238000000386 microscopy Methods 0.000 claims description 5
- 230000002596 correlated effect Effects 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims 1
- 238000010408 sweeping Methods 0.000 claims 1
- 230000003760 hair shine Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000004422 calculation algorithm Methods 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000005622 photoelectricity Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000017105 transposition Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001839 endoscopy Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000003702 image correction Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004621 scanning probe microscopy Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00002—Operational features of endoscopes
- A61B1/00057—Operational features of endoscopes provided with means for testing or calibration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
- A61B1/043—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances for fluorescence imaging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0068—Confocal scanning
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Surgery (AREA)
- Medical Informatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Radiology & Medical Imaging (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Abstract
This application discloses a kind of burnt based endoscopic imaging alignment correction system and method for copolymerization, the light beam that laser generates enters X/Y axis scanning galvanometer after the first optical filter, dichroic mirror；The light beam of X/Y axis scanning galvanometer reflection enters fiber optic bundle through beam-expanding system and coupling object lens, and light beam shines tissue and excites the fluorescence signal of tissue surface；Fluorescence signal via fiber optic bundle, coupling object lens, beam-expanding system, after X/Y axis scanning galvanometer through dichroic mirror, the second optical filter, apeture lens, pin hole and be irradiated to photodetector；Fluorescence signal is converted into electric signal by photodetector, is transmitted to computer disposal after being converted into digital signal via multichannel collecting control panel.Method, including acquisition image；The odd-numbered line of acquired image or even rows are separately constituted into piece image；It is calculated using circulation cross-correlation function；Mobile all or even rows, show after picture mosaic again.The application corrects the parity rows problem of misalignment of image under the premise of guaranteeing sampling efficiency, improves the quality of image.
Description
Technical field
The application belongs to the burnt based endoscopic imaging technical field of copolymerization, and in particular to a kind of copolymerization burnt based endoscopic imaging alignment correction system
System and method.
Background technique
Fluorescence co-focusing based endoscopic imaging technology is to be combined together optical fiber Endoscopy and confocal scanning microscopy
Living body fluorescent high-resolution imaging may be implemented in a kind of technology, is optical microscopy imaging technology and high-resolution based endoscopic imaging technology
It breaks through, will break a new path for the research of diagnosing early malignant tumor.
X-axis scanning galvanometer employed in fluorescence co-focusing based endoscopic imaging technology can form two in a round trip cycle
Row image, but due to the presence of inertia, galvanometer is gone to by backhaul has the time reversely accelerated again of slowing down to turn when backhaul
To the time, this time is with Parameters variations such as amplitude, period, temperature.Due to the presence of turnaround time, capture card is by fixed week
Phase collects pixel can not be completely corresponding with actual position, and spliced image odd-numbered line and even number line will appear mistake
Position.And since there is certain error in the time that capture card starts the time acquired and X-axis scanning galvanometer setting in motion, can aggravate
The dislocation of parity rows.The method that tradition solves parity rows image offset is to use scanning mode as shown in Figure 4, and black is real in figure
Line indicates imaging session, and black dotted lines indicate that line feed section, the data acquired in the section that enters a new line are simply discarded, X-axis scanning galvanometer return
Path the problem of will not being imaged, being thus not in the image offset of parity rows.But since X-axis scanning galvanometer is at one
Period only generates the image of a line, and scan efficiency will be greatly reduced.In order to solve the problems, such as odd even line misregistration, and keep scheming
The collecting efficiency of picture, present applicant proposes a kind of burnt based endoscopic imaging alignment correction system and methods of copolymerization.
Apply for content
The shortcomings that for the above-mentioned prior art or deficiency, the application technical problems to be solved are to provide one kind and are guaranteeing to adopt
Under the premise of sample efficiency, the parity rows problem of misalignment of image is corrected, improves the copolymerization coke based endoscopic imaging alignment correction of picture quality
System and method.
In order to solve the above technical problems, the application has following constitute:
A kind of burnt based endoscopic imaging alignment correction system of copolymerization, comprising: laser, the first optical filter, dichroic mirror, X/Y axis are swept
Retouch galvanometer, beam-expanding system, coupling object lens, fiber optic bundle, the second optical filter, apeture lens, pin hole, photodetector and multichannel
Acquisition control plate, the laser beam that the laser generates enter the X/Y axis after first optical filter, the dichroic mirror and sweep
Retouch galvanometer；The light beam of the X/Y axis scanning galvanometer reflection enters the fiber optic bundle through the beam-expanding system and the coupling object lens,
Light beam is irradiated to the different location of tissue surface, and excites the fluorescence signal of tissue surface corresponding position；The fluorescence signal
Via after the fiber optic bundle, the coupling object lens, the beam-expanding system, the X/Y axis scanning galvanometer penetrate the dichroic mirror and
Second optical filter is successively irradiated on the apeture lens, the pin hole and the photodetector；The photoelectricity is visited
It surveys device and the fluorescence signal is converted into electric signal, be converted into being transferred to calculating after digital signal via multichannel collecting control panel
Microscopy endoscopic image is generated after being handled on machine.
As a further improvement, the X/Y axis scanning galvanometer includes X-axis scanning galvanometer and Y axis scanning galvanometer, wherein
The second rotor in the first rotor and Y axis scanning galvanometer in the X-axis scanning galvanometer with the multichannel collecting control panel
X eyeglass is installed in electrical connection, the end of the first rotor, and Y eyeglass is installed in bitrochanteric end, wherein the deflection angle of X eyeglass is true
Hot spot is determined in the position of sample surfaces X-direction, and the deflection angle of Y-axis eyeglass determines hot spot in the position of sample surfaces Y-direction.
As a further improvement, the X-axis scanning galvanometer and Y axis scanning galvanometer are galvanometer galvanometer.
As a further improvement, the system uses the scanning mode of " bow " font to acquire multiple groups figure on to the sample
As signal.
As a further improvement, constant duration between each group described image signal.
As a further improvement, the imaging session of described image signal and line feed section are successively staggered, wherein adjacent institute
It states imaging session to be arranged in parallel, the adjacent line feed section is arranged in parallel.
Method based on the system, comprising: be based on the complete image of one width of system acquisition；By acquired image
Odd-line pixels extract and be reassembled into piece image, equally even rows are extracted be reassembled into it is another
Width image, then the distance of the correlated characteristic dislocation in above-mentioned two images is Δ L；It is calculated using circulation cross-correlation function；It is mobile
The pixel of even number line is individually moved all pixels, shows after stitching image again.
As a further improvement, two images cross-correlation matrix, matrix peak is calculated using circulation cross-correlation function
The difference of value point and matrix central point abscissa is the distance, delta L of two image offsets.
As a further improvement, if Δ L is even number, by the mobile L/2 position of Δ of all pixels；If Δ L is
1 pixel is individually moved behind the position of all pixels (Δ L-1)/2, then by the pixel of even number line in odd number.
As a further improvement, start acquire image at the time of and X-axis scanning galvanometer scanning initial time time
Between be divided into N number of sampling period, the turnaround time of X-axis scanning galvanometer is X sampling period, and the system is within a sampling period
A pixel is acquired, then, script is 2N+ in the distance that two features of same row are staggered in acquired image on sample
X pixel；Wherein, the Δ L=2N+X.
Compared with prior art, the application has the following technical effect that
The application is in no increase imaging device complexity, data acquisition time and under the premise of with data collection capacity, only
By the algorithm process in later period, the problem of eliminating the parity rows image offset occurred when the imaging of galvanometer galvanometer is realized；This Shen
Feature difference please based on odd-numbered line and even number line two images carries out circulation cross-correlation calculation, obtain the dislocation of two images away from
From, and by the way that whole pixel is mobile or even number line is individually moved, it ensure that image information while corrected transposition problem
Completely.
Detailed description of the invention
By reading a detailed description of non-restrictive embodiments in the light of the attached drawings below, the application's is other
Feature, objects and advantages will become more apparent upon:
Fig. 1: the application is copolymerized the structural schematic diagram of burnt based endoscopic imaging alignment correction system；
Fig. 2: the structural schematic diagram of X/Y axis scanning galvanometer in the application；
Fig. 3: the image scanning mode figure that the application uses；
Fig. 4: the image scanning mode figure used in the prior art；
Fig. 5: odd even line misregistration apart from schematic diagram in the application；
Fig. 6: the application is copolymerized the flow chart of burnt based endoscopic imaging alignment correction method；
Fig. 7: the original graph of burnt based endoscopic imaging alignment correction system compensation is copolymerized without the application；
Fig. 8: the effect picture after the application is copolymerized burnt based endoscopic imaging alignment correction system compensation.
Specific embodiment
It is described further below with reference to technical effect of the attached drawing to the design of the application, specific structure and generation, with
It is fully understood from the purpose, feature and effect of the application.
As shown in Figure 1, the present embodiment is copolymerized burnt based endoscopic imaging alignment correction system, comprising: laser, the first optical filter,
Dichroic mirror, X/Y axis scanning galvanometer, beam-expanding system, coupling object lens, fiber optic bundle, the second optical filter, apeture lens, pin hole and light
Electric explorer and multichannel collecting control panel.Wherein, the laser emits laser, and first optical filter is arranged described
In the transmitting optical path of laser, the dichroic mirror is arranged on the output light path of first optical filter and filters to described first
The light that piece issues is reflected, and the X/Y axis scanning galvanometer is arranged on the dichroiscopic reflected light path, the beam-expanding system
It is arranged on the reflected light path of the X/Y axis scanning galvanometer, the output light path of the beam-expanding system is arranged in the coupling object lens
On, the fiber optic bundle is arranged on the output light path of the coupling object lens and detects to sample, and the sample is after irradiating
It issues fluorescence signal and returns；Second optical filter is arranged on the dichroiscopic transmitted light path, the apeture lens, institute
It states pin hole and the photodetector is arranged on the output light path of second optical filter, wherein the photodetector
Electric signal is converted into the optical signal of the output of second optical filter, the multichannel collecting control panel acquires the photoelectricity and visits
Survey device electric signal and be converted into digital signal be delivered to handled on computer after generate microscopy endoscopic image.
The laser beam that the laser generates enters the X/Y axis after first optical filter, the dichroic mirror and scans
Galvanometer；The light beam of the X/Y axis scanning galvanometer reflection enters the fiber optic bundle through the beam-expanding system and the coupling object lens, will
Light beam is irradiated to the different location of tissue surface, and excites the fluorescence signal of tissue surface corresponding position；The fluorescence signal warp
By after the fiber optic bundle, the coupling object lens, the beam-expanding system, the X/Y axis scanning galvanometer successively through the dichroic mirror,
Second optical filter, the apeture lens, the pin hole are simultaneously irradiated on the photodetector；The photodetector will
The fluorescence signal is converted into electric signal, is converted into being transferred to computer after digital signal via multichannel collecting control panel enterprising
Microscopy endoscopic image is generated after row processing.
As shown in Fig. 2, the X/Y axis scanning galvanometer includes X-axis scanning galvanometer 10 and Y axis scanning galvanometer 20, wherein described
The second rotor 21 in the first rotor 11 and Y axis scanning galvanometer 20 in X-axis scanning galvanometer 10 with the multichannel collecting control
X eyeglass 12 is installed in making sheet electrical connection, the end of the first rotor 11, and Y eyeglass 22 is installed in the end of the second rotor 21, wherein X eyeglass
12 deflection angle determines hot spot in the position of sample surfaces X-direction, and the deflection angle of Y-axis eyeglass determines hot spot in sample surfaces
The position of Y-direction.Wherein, laser beam is realized in entire range to be imaged under the driving of X/Y axis scanning galvanometer on sample
The fluorescence signal scanned is spliced into complete image by scanning, computer.
Wherein, the X-axis scanning galvanometer 10 and Y axis scanning galvanometer 20 are galvanometer galvanometer.Laser beam is swept by X-axis
The deflection of optical path is realized in the reflection for retouching the Y eyeglass 22 on the X eyeglass 12 and Y axis scanning galvanometer 20 on galvanometer 10.X-axis scanning galvanometer
10 deflection angle determines hot spot in the position of sample surfaces X-direction, and the deflection angle of Y axis scanning galvanometer 20 determines hot spot in sample
The position of product surface Y-direction.Wherein, the deflection angle of the X-axis scanning galvanometer 10 and Y axis scanning galvanometer 20 is adopted by multichannel
Collect the control voltage that control panel issues to determine.
The scanning mode such as Fig. 3 of laser beam in sample surfaces under the driving of X-axis scanning galvanometer 10 and Y axis scanning galvanometer 20
It is shown, that is, the system uses the scanning mode of " bow " font to acquire multiple series of images signal on to the sample, it is preferable that each
Constant duration between group described image signal.The imaging session and line feed section of described image signal are successively staggered, wherein adjacent
The imaging session is arranged in parallel, and the adjacent line feed section is arranged in parallel.It wherein, is laterally X-direction in Fig. 3, longitudinal is Y-direction.
Solid black lines line segment is imaging session in Fig. 3, and black dotted lines section is line feed section.In X-axis scanning galvanometer 10 and Y axis scanning galvanometer 20
Capture card acquires pixel at regular intervals in motion process, and is transmitted to computer, computer by pixel in order
It is spliced into piece image.
X-axis scanning galvanometer 10 can form two row images in a round trip cycle, but due to the presence of inertia, X-axis is swept
Retouching galvanometer 10 and being gone to by backhaul has one to slow down the time i.e. turnaround time reversely accelerated again when backhaul, this time is with vibration
The Parameters variations such as width, period, temperature.Due to the presence of turnaround time, capture card collects pixel by the fixed cycle can not
Completely corresponding with actual position, spliced image odd-numbered line and even number line will appear dislocation.And due to X-axis scanning galvanometer
10 position and capture card acquisition signal start opportunity and can not correspond to completely, can aggravate the dislocation of parity rows.But this implementation
The scan efficiency of the scanning mode more as shown in Figure 4 than the prior art of scanning mode used by example is doubled, because in Fig. 4
Solid black lines indicate imaging session, and black dotted lines indicate that line feed section, the data acquired in the section that enters a new line are simply discarded, X-axis scanning vibration
The problem of path of 10 return of mirror will not be imaged, thus be not in the image offset of parity rows.But it shakes since X-axis scans
Mirror 10 only generates the image of a line in a cycle, therefore, such as Fig. 4 institute than the prior art of scanning mode used by the present embodiment
The scan efficiency for the scanning mode shown is doubled.In order to solve the problems, such as odd even line misregistration, and the acquisition of image is kept to imitate
Rate, the present embodiment use the scanning mode of Fig. 3, and reach the mesh of parity rows alignment using the algorithm of image alignment on computers
's.
As shown in figure 5, two circles are the images after the same circle dislocation in figure.The imaging of the present embodiment confocal endoscope is wrong
Bit correction system acquires N group picture signal according to scanning mode as shown in Figure 3 in sample position, whens waiting between each group picture signal
Between be spaced.But start acquire image at the time of and X-axis scanning galvanometer scanning initial time time interval be N number of sampling
Period.And the turnaround time of X-axis scanning galvanometer is X sampling period.Imaging system acquires one within a sampling period
Pixel, so in the distance that is staggered in acquired image of two features of same row being originally 2N+X pixel on sample.
As shown in fig. 6, bearing calibration of the present embodiment based on confocal endoscope image correction system, including walk as follows
It is rapid:
Step 1 is based on the complete image of one width of system acquisition；It collects data and splices image in a row until complete
At the complete image of a width.
It is swept specifically, the laser beam that laser generates enters the X/Y axis after first optical filter, the dichroic mirror
Retouch galvanometer；The light beam of the X/Y axis scanning galvanometer reflection enters the fiber optic bundle through the beam-expanding system and the coupling object lens,
Light beam is irradiated to the different location of tissue surface, and excites the fluorescence signal of tissue surface corresponding position；The fluorescence signal
Via after the fiber optic bundle, the coupling object lens, the beam-expanding system, the X/Y axis scanning galvanometer penetrate the dichroic mirror and
Second optical filter is irradiated on the photodetector；The fluorescence signal is converted into telecommunications by the photodetector
Number, be converted into being transferred to after digital signal via multichannel collecting control panel handled on computer after generate microscopy endoscopic figure
Picture.
The odd-line pixels of acquired image are extracted and are reassembled into piece image by step 2, equally will be even
Several rows of pixel extractions, which come out, is reassembled into another piece image, then the distance, delta L of the correlated characteristic dislocation in above-mentioned two images.
Two images cross-correlation matrix, matrix peak point and matrix is calculated using circulation cross-correlation function in step 3
The difference of central point abscissa is the distance, delta L of two image offsets.
Wherein, when the present embodiment corrects system acquisition N group picture signal, constant duration between each group picture signal.But
It is at the time of starting to acquire image and the time interval of the initial time of X-axis scanning galvanometer scanning is N number of sampling period, and X
The turnaround time of axis scanning galvanometer is X sampling period.Imaging system acquires a pixel within a sampling period, so sample
Script is 2N+X pixel in the distance that two features of same row are staggered in acquired image on product；Wherein, the Δ L
=2N+X.
Step 4 judges that moving all pixels, still the pixel of even number line is individually moved, to eliminate parity rows pixel
Dislocation.
Specifically, if Δ L is even number, by the mobile L/2 position of Δ of all pixels；If Δ L is odd number, will own
Behind the position of pixel (Δ L-1)/2, then 1 pixel is individually moved in the pixel of even number line, eliminates odd even line misregistration to reach
Effect.
Step 5 again after stitching image, shows image.
The image finally shown is as shown in figure 8, it has eliminated parity rows image offset problem.
As shown in Figure 7 and Figure 8, after the present embodiment corrects, image is efficiently solved under the premise of guaranteeing sampling efficiency
The problem of misalignment of parity rows, improves picture quality.
The application is handled to obtain the misplaced data of two images using two images that odd-numbered line and even number line are formed,
To handle acquisition time and X-axis scanning galvanometer position is asynchronous and image offset caused by the turnaround time of galvanometer galvanometer
Problem；Its feature difference based on odd-numbered line and even number line two images carries out circulation cross-correlation calculation, obtains two images
Dislocation distance, and ensure that image is believed by whole pixel movement or being individually moved while corrected transposition problem for even number line
What is ceased is complete；The application corrects the parity rows problem of misalignment of image under the premise of guaranteeing sampling efficiency, improves the quality of image,
With important application value.
Above embodiments are only to illustrate the technical solution of the application and non-limiting, referring to preferred embodiment to the application into
Detailed description is gone.Those skilled in the art should understand that the technical solution of the application can be modified or be waited
With replacement, without departing from the spirit and scope of technical scheme, should all cover within the scope of claims hereof.
Claims (10)
1. a kind of burnt based endoscopic imaging alignment correction system of copolymerization, which is characterized in that the system comprises:
Laser, the first optical filter, dichroic mirror, X/Y axis scanning galvanometer, beam-expanding system, coupling object lens, fiber optic bundle, second are filtered
Piece, apeture lens, pin hole and photodetector and multichannel collecting control panel,
The laser beam that the laser generates enters the X/Y axis scanning galvanometer after first optical filter, the dichroic mirror；
The light beam of the X/Y axis scanning galvanometer reflection enters the fiber optic bundle through the beam-expanding system and the coupling object lens, will
Light beam is irradiated to the different location of tissue surface, and excites the fluorescence signal of tissue surface corresponding position；
After the fluorescence signal is via the fiber optic bundle, the coupling object lens, the beam-expanding system, the X/Y axis scanning galvanometer
It successively penetrates the dichroic mirror, second optical filter, the apeture lens, the pin hole and is irradiated to the photodetector
On；
The fluorescence signal is converted into electric signal by the photodetector, is converted into digital letter via multichannel collecting control panel
Generation microscopy endoscopic image after being handled on computer is transferred to after number.
2. system according to claim 1, which is characterized in that
The X/Y axis scanning galvanometer includes X-axis scanning galvanometer and Y axis scanning galvanometer,
Wherein, the second rotor in the first rotor and Y axis scanning galvanometer in the X-axis scanning galvanometer uniformly adopt by the multichannel
Collect control panel electrical connection,
X eyeglass is installed in the end of the first rotor, and Y eyeglass is installed in bitrochanteric end, wherein the deflection angle of X eyeglass determines
For hot spot in the position of sample surfaces X-direction, the deflection angle of Y-axis eyeglass determines hot spot in the position of sample surfaces Y-direction.
3. system according to claim 2, which is characterized in that the X-axis scanning galvanometer and Y axis scanning galvanometer are inspection stream
Meter formula galvanometer.
4. system according to claim 1, which is characterized in that the system uses sweeping for " bow " font on to the sample
The mode of retouching acquires multiple series of images signal.
5. system according to claim 4, which is characterized in that constant duration between each group described image signal.
6. system according to claim 4 or 5, which is characterized in that the imaging session and line feed section of described image signal are successively
It is staggered, wherein the adjacent imaging session is arranged in parallel, and the adjacent line feed section is arranged in parallel.
7. the method based on the system as described in any one of claim 1 to 6 characterized by comprising
Based on the complete image of one width of system acquisition,
The odd-line pixels of acquired image are extracted and are reassembled into piece image, equally extract even rows
It is reassembled into another piece image out, then the distance, delta L of the correlated characteristic dislocation in above-mentioned two images；
It is calculated using circulation cross-correlation function；
The pixel of even number line is individually moved mobile all pixels, shows after stitching image again.
8. the method according to the description of claim 7 is characterized in that it is mutual that two images are calculated using circulation cross-correlation function
The difference of correlation matrix, matrix peak point and matrix central point abscissa is the distance, delta L of two image offsets.
9. according to the method described in claim 8, it is characterized in that, if Δ L is even number, by the mobile Δ L/2 of all pixels
A position；If Δ L is odd number, 1 is individually moved behind the position of all pixels (Δ L-1)/2, then by the pixel of even number line
Pixel.
10. according to the method described in claim 9, it is characterized in that, being scanned at the time of starting to acquire image with X-axis scanning galvanometer
The time interval of initial time be N number of sampling period, turnaround time of X-axis scanning galvanometer is X sampling period, the system
A pixel is acquired within a sampling period, then, originally in two features of same row in acquired image on sample
On the distance that is staggered be 2N+X pixel；Wherein, the Δ L=2N+X.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811583549.2A CN109645936B (en) | 2018-12-24 | 2018-12-24 | Confocal endoscopic imaging dislocation correction system and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811583549.2A CN109645936B (en) | 2018-12-24 | 2018-12-24 | Confocal endoscopic imaging dislocation correction system and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109645936A true CN109645936A (en) | 2019-04-19 |
| CN109645936B CN109645936B (en) | 2023-12-12 |
Family
ID=66116533
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811583549.2A Active CN109645936B (en) | 2018-12-24 | 2018-12-24 | Confocal endoscopic imaging dislocation correction system and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109645936B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111415312A (en) * | 2020-04-08 | 2020-07-14 | 中国科学院苏州生物医学工程技术研究所 | Confocal endoscope image processing method, system and computer equipment |
| CN112890736A (en) * | 2019-12-03 | 2021-06-04 | 精微视达医疗科技（武汉）有限公司 | Method and device for obtaining field mask of endoscopic imaging system |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020057492A1 (en) * | 1999-03-19 | 2002-05-16 | Olympus Optical Co., Ltd. | Scanning confocal microscope |
| US20020198457A1 (en) * | 2001-04-30 | 2002-12-26 | Tearney Guillermo J. | Method and apparatus for improving image clarity and sensitivity in optical coherence tomography using dynamic feedback to control focal properties and coherence gating |
| CN101449963A (en) * | 2008-12-29 | 2009-06-10 | 浙江大学 | Laser co-focusing micro-endoscope |
| JP2012080490A (en) * | 2010-10-06 | 2012-04-19 | Nikon Corp | Imaging device and image processing device |
| CN102841083A (en) * | 2012-06-11 | 2012-12-26 | 北京大学 | Method and system of laser scanning phase-microscope imaging |
| CN203232047U (en) * | 2013-05-10 | 2013-10-09 | 东南大学 | Flowing two-dimensional velocity field measuring device with interlaced scanning CCD |
| CN104568873A (en) * | 2014-12-22 | 2015-04-29 | 中国科学院苏州生物医学工程技术研究所 | Laser scanning confocal microscope for imaging fluorescent substances |
| CN104837436A (en) * | 2012-11-01 | 2015-08-12 | 阿莱恩技术有限公司 | Motion compensation in a three dimensional scan |
| WO2016032432A1 (en) * | 2014-08-26 | 2016-03-03 | FARKAS, Daniel | Method and system for heterodyned fluorescence tomography |
| CN105796044A (en) * | 2016-05-25 | 2016-07-27 | 珠海康弘发展有限公司 | Endoscope and imaging method thereof |
| CN106769876A (en) * | 2016-11-28 | 2017-05-31 | 电子科技大学 | The new scan method and its device of a kind of optical resolution photoacoustic microscope |
| CN107065157A (en) * | 2017-02-21 | 2017-08-18 | 中国科学院苏州生物医学工程技术研究所 | A kind of co-focusing imaging distortion correction system and method |
| CN108171666A (en) * | 2017-12-27 | 2018-06-15 | 华中科技大学鄂州工业技术研究院 | A kind of sinusoidal fault image antidote for confocal endoscope |
| CN108245126A (en) * | 2018-03-26 | 2018-07-06 | 精微视达医疗科技（武汉）有限公司 | It is a kind of can be with the sonde-type co-focusing micro-endoscope of laser ablation |
| CN209951205U (en) * | 2018-12-24 | 2020-01-17 | 中国科学院苏州生物医学工程技术研究所 | Confocal endoscopic imaging dislocation correction system |
-
2018
- 2018-12-24 CN CN201811583549.2A patent/CN109645936B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020057492A1 (en) * | 1999-03-19 | 2002-05-16 | Olympus Optical Co., Ltd. | Scanning confocal microscope |
| US20020198457A1 (en) * | 2001-04-30 | 2002-12-26 | Tearney Guillermo J. | Method and apparatus for improving image clarity and sensitivity in optical coherence tomography using dynamic feedback to control focal properties and coherence gating |
| CN101449963A (en) * | 2008-12-29 | 2009-06-10 | 浙江大学 | Laser co-focusing micro-endoscope |
| JP2012080490A (en) * | 2010-10-06 | 2012-04-19 | Nikon Corp | Imaging device and image processing device |
| CN102841083A (en) * | 2012-06-11 | 2012-12-26 | 北京大学 | Method and system of laser scanning phase-microscope imaging |
| CN104837436A (en) * | 2012-11-01 | 2015-08-12 | 阿莱恩技术有限公司 | Motion compensation in a three dimensional scan |
| CN203232047U (en) * | 2013-05-10 | 2013-10-09 | 东南大学 | Flowing two-dimensional velocity field measuring device with interlaced scanning CCD |
| WO2016032432A1 (en) * | 2014-08-26 | 2016-03-03 | FARKAS, Daniel | Method and system for heterodyned fluorescence tomography |
| CN104568873A (en) * | 2014-12-22 | 2015-04-29 | 中国科学院苏州生物医学工程技术研究所 | Laser scanning confocal microscope for imaging fluorescent substances |
| CN105796044A (en) * | 2016-05-25 | 2016-07-27 | 珠海康弘发展有限公司 | Endoscope and imaging method thereof |
| CN106769876A (en) * | 2016-11-28 | 2017-05-31 | 电子科技大学 | The new scan method and its device of a kind of optical resolution photoacoustic microscope |
| CN107065157A (en) * | 2017-02-21 | 2017-08-18 | 中国科学院苏州生物医学工程技术研究所 | A kind of co-focusing imaging distortion correction system and method |
| CN108171666A (en) * | 2017-12-27 | 2018-06-15 | 华中科技大学鄂州工业技术研究院 | A kind of sinusoidal fault image antidote for confocal endoscope |
| CN108245126A (en) * | 2018-03-26 | 2018-07-06 | 精微视达医疗科技（武汉）有限公司 | It is a kind of can be with the sonde-type co-focusing micro-endoscope of laser ablation |
| CN209951205U (en) * | 2018-12-24 | 2020-01-17 | 中国科学院苏州生物医学工程技术研究所 | Confocal endoscopic imaging dislocation correction system |
Non-Patent Citations (2)
| Title |
|---|
| YANG, XB等: "Confocal Laser-Induced Fluorescence Detection System for a Microfluidic Chip Based on an Embedded System", 《LASERS IN ENGINEERING》, vol. 23, no. 3, pages 245 - 254 * |
| 张红明: "基于光纤束的共聚焦荧光内窥成像研究", 《中国博士学位论文全文数据库 (医药卫生科技辑)》, pages 080 - 16 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112890736A (en) * | 2019-12-03 | 2021-06-04 | 精微视达医疗科技（武汉）有限公司 | Method and device for obtaining field mask of endoscopic imaging system |
| CN112890736B (en) * | 2019-12-03 | 2023-06-09 | 精微视达医疗科技（武汉）有限公司 | Method and device for obtaining field mask of endoscopic imaging system |
| CN111415312A (en) * | 2020-04-08 | 2020-07-14 | 中国科学院苏州生物医学工程技术研究所 | Confocal endoscope image processing method, system and computer equipment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109645936B (en) | 2023-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10602087B2 (en) | Image acquisition device, and imaging device | |
| US9088729B2 (en) | Imaging apparatus and method of controlling same | |
| CN102439416B (en) | String line scanning encoding multicolor fluorescent microscopy and imaging flow cytometer | |
| CN101449963B (en) | Laser co-focusing micro-endoscope | |
| CN104471462A (en) | Multi-focal structured illumination microscopy systems and methods | |
| CN109645936A (en) | A kind of burnt based endoscopic imaging alignment correction system and method for copolymerization | |
| JPWO2004072624A1 (en) | Fluorescence correlation spectroscopy analyzer | |
| CN110836877A (en) | Light section microscopic imaging method and device based on liquid crystal zoom lens | |
| CN106405826A (en) | Galvanometer scanning system and scanning method for dual optical path imaging | |
| CN111220615A (en) | Inclined three-dimensional scanning microscopic imaging system and method | |
| CN106949961B (en) | Luminous power monitoring in real time and feedback method and device | |
| CN209951205U (en) | Confocal endoscopic imaging dislocation correction system | |
| JP2010256530A (en) | Microscope device | |
| US8004725B2 (en) | Device and method for scanning a document | |
| CN109820471A (en) | A kind of burnt based endoscopic imaging alignment correction system and method for copolymerization | |
| CN211324855U (en) | Confocal endoscopic imaging dislocation correction system | |
| CN106710378A (en) | Full automatic control system for femtosecond time-resolved photoelectron/ion velocity imaging | |
| CN107850766A (en) | System and method for the image procossing in light microscope | |
| CN110766620A (en) | Confocal endoscope image distortion correction method based on optical fiber probe | |
| CN109884053A (en) | Harmonic wave microscopic measuring method based on the micro- and confocal slit detection of piece light | |
| US20210311292A1 (en) | High-throughput optical sectioning imaging method and imaging system | |
| CN106841141A (en) | A kind of fiber optic loop battle array resonance type piezoelectric scanning method and device based on photon restructuring | |
| CN205620293U (en) | High -speed microscopy images collection system based on application | |
| CN108989676A (en) | A kind of autofocus and auto focusing method for increasing reflecting element and improving the depth of field | |
| CN108833788A (en) | A kind of Oblique images capture equipment improves the autofocus and auto focusing method of the depth of field |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20190419 Assignee: SHENYANG SHENDA ENDOSCOPE Co.,Ltd. Assignor: Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Contract record no.: X2020980005472 Denomination of invention: A confocal endoscope imaging dislocation correction system and method License type: Common License Record date: 20200827 |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |