CN109632666A - The method of portable multi-channel spectrophotometer and measurement absorbance based on smart phone - Google Patents
The method of portable multi-channel spectrophotometer and measurement absorbance based on smart phone Download PDFInfo
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- CN109632666A CN109632666A CN201811579162.XA CN201811579162A CN109632666A CN 109632666 A CN109632666 A CN 109632666A CN 201811579162 A CN201811579162 A CN 201811579162A CN 109632666 A CN109632666 A CN 109632666A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02D—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN INFORMATION AND COMMUNICATION TECHNOLOGIES [ICT], I.E. INFORMATION AND COMMUNICATION TECHNOLOGIES AIMING AT THE REDUCTION OF THEIR OWN ENERGY USE
- Y02D30/00—Reducing energy consumption in communication networks
- Y02D30/70—Reducing energy consumption in communication networks in wireless communication networks
Abstract
The present invention relates to the portable multi-channel spectrophotometer based on smart phone and the methods for measuring absorbance, the spectrophotometer includes being protected from light magazine, light source, cell rack, measurement analytical unit, the measurement analytical unit is the smart phone for being provided with camera, the cell rack is located between light source and measurement analytical unit, so that the camera face cell rack of measurement analytical unit；The light source and cell rack setting are being protected from light in magazine, and the measurement analytical unit setting is being protected from light outside magazine.The present invention uses smart phone as the measurement of monochromatic source generator, light intensity signal detector, image grayscale and ratio and data platform, it is simple with accuracy height, structure, it is small in size, light-weight, low energy consumption, the advantages of easy to disassemble and carrying, and it is low in cost, expansibility is strong, easy to operate.
Description
Technical field
The invention belongs to analysis detection fields, and in particular to a kind of portable multi-channel spectrophotometer and measurement absorbance
Method, the spectrophotometer is by intelligent monochromatic source module, multi-channel detection pond, smart phone and peace based on RGB mode
Self-editing image processing software composition on smart phone.
Background technique
Spectrophotometer is a kind of common analysis instrument, is widely used in various analysis detections.Its basic principle
It is a branch of collimated monochromatic ligth of measurement through the variation of its intensity after solution, quantitative basis is Lambert-beer's law, it may be assumed that
In formula (1), A is absorbance, I0For the intensity of incident light, I is the intensity of transmitted light, and ε is extinction material in solution
Molar absorption coefficient under the wavelength (λ), b are the length for absorbing light path, and C is the molar concentration of extinction material.
When measuring solution absorbance, to eliminate loss of the light reflection of cuvette with absorption to incident intensity, and
Influence of the solvent to light absorption is usually arranged reference solution and returns to zero, i.e., zero artificially set by the absorbance of reference solution, with this
For reference point, the absorbance of extinction material in solution is measured, calculates its concentration according to formula (1).According to apparatus structure, list can be divided into
The measurement methods such as channel, binary channels, dual wavelength.The spectrophotometer of early stage mostly uses single-channel, such as domestic 72 type, 721 types,
751 type spectrophotometers, the light issued by light source, by monochromator (prism), slit, convex lens etc., the list of wavelength needed for obtaining
Coloured light, by measuring luminous intensity by battery (72 type), photoelectric tube (721,751) after colorimetric pool, and by reference solution light transmittance (T
=I0/ I) it is adjusted to 100%, i.e. A=0, then measure the absorbance of sample solution.It can be seen that although single has
The simple advantage of instrument and equipment, but surveyed absorbance is affected by intensity of light source fluctuation, spectrophotometer usually require compared with
Long preheating time, and have higher requirement to the stability of power supply.To overcome the above-mentioned deficiency of single-channel spectrophotometer,
Current most of spectrophotometers use two pass bands mode.The collimated monochromatic ligth exported by monochromator, through chopper one
It is divided into two, respectively by reference solution and sample solution, absorbance is calculated with the ratio between its luminous intensity.Therefore, binary channels is divided light
Even if degree meter remains to Accurate Determining absorbance, this is provided favorably for rapid spectrum scanning in the case where intensity of light source variation
Condition.In addition, binary channels spectrophotometer generallys use grating as single scattered element, photomultiplier tube is detector, computer
Data, which are carried out, for control system adopts set record, the price for thereby resulting in spectrophotometer is soaring, and equipment instrument and weight increase,
And power consumption is very high, mainly uses in the lab, the field quick detection of unsuitable non-lab environment, therefore researches and develops one
Kind structure is simple, easy to operate, low in cost, and accuracy is high, and miniaturization is easy to carry, can be used for the light splitting of field quick detection
Photometer is necessary.
In recent years, smart phone has been popularized, and under fierce market and competitive mechanisms, all big enterprises are continued to introduce new,
If the arithmetic speed of central processing unit is getting faster, memory capacity is increasing, and data exchange capability is more and more stronger, and cost performance is got over
Come higher.Smart phone has become palmtop and digital camera combination at present, in addition to possessing basic communication function,
In various sensors and application software, keep the function of smart phone stronger and stronger, the purposes to develop new has created
The condition of power.Wherein, the analysis measuring device development based on smart phone is concerned again.
For example, the built-in camera configured using smart phone, can very easily carry out the capture of optical signalling, and
Luminous intensity measurement is carried out with mobile phone application software (APP), can be used for based on absorbance, fluorescence, (electricity) chemiluminescent sensing inspection
Survey analysis.Wherein, the colorimetric device based on smart phone, spectrophotometer have document report.For example, Grasse etc. is reported
Spectrophotometer as shown in Figure 1 (E.K.Grasse, et al., J.Chem.Educ., 2016,93,146-151), have with
Common spectrophotometer similar sensitivity and spectral resolution, but the device absorbed using complex light after use diffraction grating
It is divided, in addition to structure is complex, the mode of measurement is still single channel, the luminous intensity of sample solution and reference solution
It can measure, be affected by intensity of light source fluctuation respectively.Hussain etc. devises absorbance measurement device as shown in Figure 2
(I.Hussain et al., Sens.Actuators B, 2017,239,1042-1050), uses the headlamp in smart phone
For light source, by fiber optic transmission into colorimetric pool, with the variation of exposed light transducer measurement luminous intensity, which is also single-pass
Road measurement pattern, absorbance measured value are equally influenced by intensity of light source variation, and are changed by replacement optical filter and absorbed wave
It is long, both be inconvenient to operate and wavelength accuracy or poor, absorption light path is single.
Although absorbance or spectrophotomelric assay device based on smart phone have had many document reports, these dresses
Setting all is to use single channel mode determination, and the luminous intensity of sample and reference solution cannot measure simultaneously, it is difficult to eliminate the intensity of light source
Drift bring detection error, this error is particularly evident under conditions of using portable simple light source, influences its measurement knot
The accuracy of fruit.
Summary of the invention
Larger for commodity spectrophotometer instrument volume described in the prior art, power consumption is high, is not suitable for field condition
Analysis is insufficient, and the absorbance measurement device based on smart phone accuracy by intensity of light source influence of fluctuations status, this
The method that invention proposes a kind of the portable multi-channel spectrophotometer based on smart phone and measurement absorbance, absorbance is not
It is influenced again by intensity of light source fluctuation, and can very easily switch monochromatic wavelength, absorb light path.
Spectrophotometer designed by the present invention has the advantages that function is strong, accuracy is high, and structure is simple, volume
Small light-weight, low in energy consumption, manufacture craft is simple, low in cost, easy to carry, is applicable in spectrophotometry on site, can incite somebody to action
Visual colorimetric determination semi-quantitative analysis promotes the level to spectrophotometric method quantitative measurement.
Technical scheme is as follows:
A kind of portable multi-channel spectrophotometer based on smart phone, including be protected from light magazine, light source, cell rack,
Analytical unit is measured, the measurement analytical unit is the smart phone for being provided with camera, and the cell rack is located at light
Between source and measurement analytical unit, so that the camera face cell rack of measurement analytical unit；The light source and cuvette
Frame setting is being protected from light in magazine, and the measurement analytical unit setting is being protected from light outside magazine.Magazine is protected from light to mention for absorbance measurement
For dark surrounds, the interference of ambient is eliminated.Preferably, the size for being protected from light magazine is 180 × 120 × 75mm, and material is black
Color plastics.
, according to the invention it is preferred to, the magazine that is protected from light is provided with the first fixing clamp, is used for fixed light source；Described
It is protected from light magazine and is provided with the second fixing clamp, be used for fixation measuring analytical unit.
, according to the invention it is preferred to, more than two cuvette slots are arranged in parallel in the cell rack, for placing ratio
Color ware；
Preferably, sample cell and reference cell are set in the cell rack, and the sample cell and reference cell are ratio
Color ware；The quantity of the sample cell is two or more, and further preferred 2-7 is a.Cuvette used is to mark made of glass
Quasi- product, absorbing light path can are as follows: 10,20,30,50,100mm, but all absorption light path phases than ware are required in same group of experiment
Deng.Preferably, cuvette external dimensions is 34 × 14 × 45mm, absorbs light path 30mm, liquor capacity 9mL.
, according to the invention it is preferred to, it is additionally provided between the cell rack and the camera for measuring analytical unit wide
Angle mirror.The advantage that wide-angle lens is arranged is that all cuvettes can be made uniformly to be imaged, reduces as caused by the difference of cuvette position
Luminance difference.
, according to the invention it is preferred to, the light source is intelligence GRB virtually monochromatic optical generator, further preferably intelligence
The display screen of energy mobile phone, when the display screen of smart phone is opened, the light of sending is as light source；
Luminous zone is set on wallpaper pattern it is further preferred that showing in smart phone, wallpaper pattern rest part is set as
Black, to reduce the influence of backscatter light；It is furthermore preferred that luminous zone is one group of rectangular patterns, fill opposite with absorbing wavelength
The rgb value answered, the cuvette array in the number of luminous zone, area, position and cell rack match.In the present invention, it is used for
PowerPoint software design can be used by exporting monochromatic wallpaper pattern, and monochromatic light emitting region is rectangle, with group luminous zone area
Identical as shape, the position of position and cuvette matches.It can be closed according to the virtual wavelength and rgb value function of Dan Bruton
Rgb value corresponding to virtual wavelength is arranged in system, and the virtual wavelength and rgb value functional relation of Dan Bruton is as shown in Figure 5.
According to the present invention, using the method for the portable multi-channel spectrophotometric determination absorbance based on smart phone,
It comprises the following steps that
(1) reference solution, sample solution are added separately to reference cell, in sample cell, are then placed on cell rack；
(2) the loading mobile phone wallpaper file on the smart phone display screen as light source, luminous zone face is joined on wallpaper
Than the position of solution, sample solution, the rgb value of luminous zone color is corresponding with required absorbing wavelength, is made with the light of luminous zone
For virtual monochromatic source, reference solution, sample solution are irradiated simultaneously respectively；
(3) image of reference solution, sample solution, meter are shot simultaneously with the camera of the smart phone of measurement analytical unit
The gray value of reference solution, sample solution image is calculated, theoretically absorbance A can be calculated by formula (2):
But when solution brightness of image is very high, it is in non-linear relation between gray value of image and luminous intensity, need to carries out non-thread
Property correction, the present invention propose using formula (3) calculate absorbance A, keep the range of linearity of standard curve wider；
In formula (2), (3), H0For the gray value of reference solution image, H is the gray value of sample solution image.
According to Fig. 5, the rgb value of (2) luminous zone color is changed the step, absorbing wavelength needed for replacing, monochromatic source repeats
Step (2), (3), can measure the absorbance A under different wave length.
According to the present invention, calculate reference solution in step (3), the gray value of sample solution image can be soft by picture editting
Part calculates, and preferably carries out image grayscale measurement with the cell phone application software based on android system of editor, can also pass picture
It is sent in remote computer, image grayscale measurement is carried out by computer graphics analysis software such as Matalab etc., calculates absorbance value
And it feeds back.
According to the present invention, when one pack system measures, the absorbance of one group of standard solution is first measured, standard curve is prepared, is asked
The regression equation for obtaining concentration and absorbance, is stored in smart phone, the absorbance of sample to be tested is then measured, by regression equation
Calculator concentration, display analysis is as a result, and store result.In spectroscopic assay, by the sequence of wavelength from low to high, cut
Display wallpaper of the intelligent GRB virtually in monochromatic optical generator is changed, measures its absorbance under each virtual wavelength.
In the present invention, using bis- (or more) cuvettes, reference solution and sample solution are contained respectively, is remembered simultaneously with camera
The image of each solution is recorded, with the intensity of sample solution and reference solution image than calculating absorbance, the variation of the intensity of light source is to sample
Product solution is identical with reference solution, does not influence its transmitted intensity ratio, to improve the accuracy of absorbance measurement.
On the mobile phone display screen as light source, loading mobile phone wallpaper file, face reference solution, sample are molten on wallpaper
The position of liquid, design rectangle luminous pattern, the rgb value of color is corresponding with required absorbing wavelength, using image it is bright as
Incident wavelength irradiates reference solution, sample solution simultaneously respectively.Its black background of other parts, to reduce spectrophotometer
Bias light, while also reducing the energy consumption of light source, the time is used after extending primary charging.By replacing different RGB histogram walls
Paper changes light absorbing wavelength, realizes spectrophotometry.
The image for shooting reference solution, sample solution simultaneously with the smart phone camera for being equipped with additional wide-angle lens, is used in combination
The gray scale of reference solution, sample solution image is measured from the cell phone application image analysis software based on android system of edlin
Value theoretically can calculate absorbance by formula (2):
In formula (2), H0For the gray value of reference solution, H is the gray value of sample solution；
But when solution brightness of image is very high, it is in non-linear relation between gray value of image and luminous intensity, need to carries out non-thread
Property correction, the present invention propose using formula (3) calculate absorbance, keep the range of linearity of standard curve wider.
Fig. 3 and Fig. 4 is the knot of the portable multi-channel spectrophotometer designed by the present invention based on smart phone respectively
Structure schematic diagram and pictorial diagram.The monochrome of wavelength needed for generating absorbance measurement as light source (intelligent GRB virtually monochromatic optical generator)
Light, the function are completed by smart phone, the basic principle is that any one color can be by RGB (RGB) three primary colors mixing group
At one group of rgb value will correspond to a certain specific color in turn, be equivalent to the monochromatic light of a certain wavelength, pass through and some column are arranged
Specific rgb value can be obtained the virtual monochromatic light within the scope of 380~760nm.The corresponding cell phone wallpaper specially designed in luminous zone
Middle rectangular patterns, wavelength switching can realize that only pattern color is different by replacement same size cell phone wallpaper.It needs to illustrate
It is that the realization of above functions is lower to mobile phone CPU requirement, even if eliminating the smart phone to get off is attained by requirement.
Two (more) monochromic beams that mobile phone display screen issues, be radiated at respectively with the reference cell of upright position at display screen,
On sample cell.Cell rack is four-way in Fig. 4, and settable a luminous zone is equipped with a cuvette, channel absorbance can be realized
While measure.Expand cuvette quantity if necessary, can also increase, realizes the measurement of more multichannel absorbance.Cuvette used
For the standard items made of glass, absorbing light path can are as follows: 10,20,30,50,100mm, but require to own in same group of experiment
Than the absorption equivalent optical path of ware.To reduce the photosensitive difference in cuvette position, in the front of smart phone camera, one is installed additional
All solution are imaged uniformly in a wide-angle lens, reduce the difference as caused by cuvette position.Image taking is by smart phone
It completes, data processing from the cell phone application image analysis software based on android system of edlin by completing.
According to the present invention, a kind of preferred embodiment, comprises the following steps that
(1) reference solution, sample solution are added separately to reference cell, in sample cell, then cuvette is placed in colorimetric
On ware frame；
(2) it opens for generating monochromatic smart phone, recalls required wallpaper pattern, it is ensured that the zone position that shines and colorimetric
The position of ware matches, when necessary the position of the fine-tuning smart phone, selects corresponding wallpaper to number according to absorbing wavelength；
(3) it closes and is protected from light magazine, open smart phone camera and image taking is carried out to detection zone, to reduce solution image
The background value of gray scale proposes to use shorter time for exposure (such as 0.1s) and low CCD luminous sensitivity ISO value (100), lead to
It overregulates and generates monochromatic smart phone brightness of display screen, make the average gray value of reference solution image 180 ± 10；It is fixed
Shooting focal length and exposure parameter are acquired solution image, and are stored in the built-in storage of smart phone；
(4) start cell phone application, it is molten to calculate sample according to formula (3) for the gray value of measurement sample solution, reference solution image
The absorbance of liquid；Determinand in solution can be calculated according to the regression equation of the standard curve between absorbance and material concentration
Concentration, and show on the screen；
(5) it opens and is protected from light magazine, after replacing the solution in sample cell, closing is protected from light magazine, then carries out next group of solution
The shooting of image and Data Analysis Services.
Compared with prior art, the invention has the following advantages:
1, the portable multi-channel spectrophotometer based on smart phone designed by the present invention, by smart phone as intelligence
Energy monochromatic light synthesis module, cuvette array, wide-angle lens (nonessential), smart phone, composition, structure is simple, easy to operate；Intelligence
Data processing can be carried out with image processing software in mobile phone.
2, the present invention emits the monochromatic light of specific wavelength using smart phone display screen, and can be same in one group of specified region
When provide multi beam directional light, compared with commercial spectrophotometer, without using monochromator, slit, chopper, mirror optics device
Multi-channel measuring can be realized in part.
3, the spectrophotometer designed by the present invention, cuvette are placed between incident light source and detector, and centre is not necessarily to
Other optical elements enormously simplify the design of spectrophotometer.In addition, it is not necessary that light emitting region is big using slit, shape rule
It is whole, the utilization efficiency of light source is substantially increased, the intensity needs of measurement absorbance are sufficient for using the light quantity of normal display screen.
Therefore the long cuvette for absorbing light path can be used, such as the cuvette of 50mm, 100mm, the detection for improving spectrophotometry is sensitive
Degree.
4, compared with being reported at present based on smart phone absorbance detection device, the present invention realizes multi-channel measurement mould
Formula eliminates influence of the incident light source strength fluctuation to absorbance measurement.And structure is more simple, and wavelength switching is more square
Just, spectrophotometry, simultaneous determination of multiponents can be realized.And when the cuvette of the different light paths of replacement, without changing other
Parameter is set.
5, compared with reported at present based on smart phone absorbance detection device, present invention employs correcting image ashes
To algorithm nonlinear between light exposure, the range of linearity of surveyed absorbance has been expanded significantly.
6, whole device volume is small, and light-weight, structure is simple, easy to disassemble and carrying, easy to operate, does not need independent confession
Electricity is suitble to field in-situ analysis.In addition, the spectrophotometer, low in cost, can expanding function it is strong, can be used for outlying less-developed
The fields such as the spectrophotometry in area and experimental teaching, family health care measurement, have a good application prospect.
Detailed description of the invention
Fig. 1 is the structural representation of the simple spectrophotometer based on smart phone of the designs such as Grasse in the prior art
Figure.
Fig. 2 is the structural schematic diagram of the absorbance measurement based on smart phone of the designs such as Hussain in the prior art.
Fig. 3 is the portable multi-channel spectrophotometer agent structure schematic diagram of the invention based on smart phone.
Fig. 4 is that the present invention is based on the photos in kind of the portable multi-channel spectrophotometer of smart phone.
Fig. 5 is the virtual wavelength and rgb value function relation figure according to Dan Bruton.
Fig. 6 by the embodiment of the present invention 2 survey methyl orange solution absorption spectrum and its and conventional spectrophotometers ratio
Compared with.
The standard curve and its solution image of free chlorine in the tap water surveyed in Fig. 7 this embodiment of the present invention 3.
Wherein: 1, first fixing clamp, 2, light source, 3, luminous zone, 4, be protected from light magazine, 5, cell rack, 6, sample cell, 7, ginseng
Than pond, 8, wide-angle lens, the 9, second fixing clamp, 10, camera, 11, measurement analytical unit.
Specific embodiment
Below by specific embodiment and in conjunction with attached drawing, the invention will be further described, but not limited to this.
It is right in embodiment by taking the standard curve for the chlorine that dissociates in the absorption spectrum and tap water that measure methyl orange solution as an example
The specific embodiment of portable multi-channel spectrophotometer based on smart phone of the invention, is described in detail.
Concentration as described in the examples is mass percent concentration.
Embodiment 1
As shown in figure 3, a kind of portable multi-channel spectrophotometer based on smart phone, including it is protected from light magazine 4, light source
2, cell rack 5, measurement analytical unit 11, the measurement analytical unit 11 is the smart phone for being provided with camera 10, institute
The cell rack 5 stated is located between light source 2 and measurement analytical unit 11, so that the camera 10 of measurement analytical unit 11 just compares
Color ware frame 5；The light source 2 and the setting of cell rack 5 are being protected from light in magazine 4, and the setting of measurement analytical unit 11 is being kept away
Outside brightness box 4.It is protected from light magazine 4 and provides dark surrounds for absorbance measurement, eliminate the interference of ambient.Preferably, it is protected from light dark
The size of box 4 is 180 × 120 × 75mm, and material is black plastic.
It is protected from light magazine 4 described in the present embodiment and is provided with the first fixing clamp 1, is used for fixed light source 2；Described being protected from light is dark
Box 4 is provided with the second fixing clamp 9, is used for fixation measuring analytical unit 11；
Multiple cuvette slots are arranged in parallel in the cell rack 5, for being placed in parallel cuvette；
Sample cell 6 and reference cell 7 are set in the cell rack 5, and the sample cell 6 and reference cell 7 are colorimetric
Ware；The quantity of the sample cell 6 is two.Cuvette used is the standard items made of glass, and absorbing light path can are as follows: 10,
20,30,50,100mm, but all absorption equivalent optical paths than ware are required in same group of experiment.Cuvette external dimensions is 34
× 14 × 45mm absorbs light path 30mm, liquor capacity 9mL.
Wide-angle lens 8 is additionally provided between the cell rack 5 and the camera 10 for measuring analytical unit 11.Wide-angle is set
The advantage of mirror 8 is that all solution can be made uniformly to be imaged, and reduces the difference as cuvette position caused by different.
The light source 2 is intelligence GRB virtually monochromatic optical generator, the preferably display screen of smart phone, smart phone
Display screen open when, the light of sending is as light source；It is shown in smart phone and designs luminous zone 3, wallpaper figure on wallpaper pattern
Case rest part is set as black, to reduce the influence of backscatter light；Luminous zone 3 is one group of rectangular patterns, filling and absorption wave
Long corresponding rgb value, the cuvette array in the number of luminous zone 3, area, position and cell rack 5 match.
Embodiment 2, methyl orange solution absorption spectrum measurement
Methyl orange standard solution is selected, the spectrally resolved ability of spectrophotometer of the invention is tested.
(1) in the present embodiment, the size for being protected from light magazine is 180 × 120 × 75mm, and material is black plastic.2 institute of light source
The mobile phone model used is Huawei's honor six, for exporting monochromatic wallpaper pattern PowerPoint software design, single-shot
Light region is rectangle, and identical as shape with group luminous zone area, the position of position and cuvette matches.
According to the virtual wavelength of Dan Bruton and rgb value functional relation (Fig. 5), RGB corresponding to virtual wavelength is set
Value.It is 400~560nm, wavelength interval 10nm that the present embodiment, which chooses virtual range of wavelengths,.Glass cuvette external dimensions is 34
× 14 × 45mm absorbs light path 30mm, liquor capacity 9mL.Shoot smart phone model Huawei honor used in image
Seven, rear camera is 20,000,000 pixels.
It is first turned on smart phone of the spectrophotometer of the present invention as light source, it is molten to place reference in two cuvettes
Liquid, recalls wallpaper pattern, observation luminous zone whether with cuvette location matches, shoot solution image, measure the ash of two pond solution
Whether degree is very close, and the adjustment of luminous zone position need to be carried out when difference is larger.
(2) preparation of methyl orange solution: weighing methyl orange 50mg, is dissolved in 100mL deionized water, and concentration is
0.05%, take 1mL to be diluted with water to 100mL, concentration 0.0005% first uses UV-1700 spectrophotometer, is ginseng with water
Than measuring its absorption spectrum as the standard compared, absorption light path used is 10mm.The methyl orange for taking concentration to be 0.0005% is molten
Liquid 10mL, water are diluted to 30mL, and 0.00017%, it is ginseng with water with multichannel spectrophotometer and 30mm cuvette of the invention
Than shooting under certain virtual wavelength illumination, then monochromatic wave is replaced in the image of reference solution and methyl orange solution and storage
It is long, carry out next measurement.
(3) it opens the cell phone application image processing software based on android system write in smart phone and will be deposited
Reference solution, sample solution image successively import under the different wave length of storage, measure its gray value, and right back-pushed-type (3) calculates through non-
Absorbance after linearity correction stores wavelength and absorbance data.It is disposed to all pictures, using wavelength as abscissa, inhales
Luminosity is ordinate, obtains the absorption spectrum of methyl orange, as shown in Figure 6.As seen from Figure 6, the present invention designed by based on intelligent hand
The portable multi-channel spectrophotometer of machine, surveyed absorption spectrum is close with the large-scale measurement result of spectrophotometer, i.e., also has
Good spectral resolution.
The measurement of free chlorine in embodiment 3, tap water
(1) principle: the measurement of tap water free chlorine, referring to state environment protecting standard (HJ 586-2010).Its principle
It is under the conditions of pH is 6.2~6.5, free chlorine directly reacts with N, N- diethyl-Isosorbide-5-Nitrae-phenylenediamine (DPD), generates red
Color compound measures its absorbance using spectrophotometry under 515nm wavelength.Since free chlorine standard solution is unstable and
It is not easy to obtain, which replaces free chlorine to do calibration curve with iodine molecule.On the basis of Potassiumiodate, in acid condition with iodate
Following reaction occurs for potassium:
IO3 -+5I-+6H+=3I2+3H2O, I2+I-=[I3]-,
The iodine molecule or [I of generation3]-With DPD generation chromogenic reaction, the ratio of the amount of the substance of iodine molecule and chlorine molecule is closed
System is 1:1.
(2) operating procedure that operating process is recommended according to HJ 586-2010, Potassiumiodate are prepared dense relative to chlorine quality
Spend ρ (Cl2) be respectively 0.00,0.10,0.20,0.30,0.40,0.50,0.60 and 0.80mg/L standard solution, it is aobvious with DPD
After color, portable multi-channel spectrophotometric determination its absorbance based on smart phone designed with the present invention, cuvette light
Journey is 50mm, and measurement wavelength is 515nm, and gained standard curve is as shown in fig. 7, as seen from Figure 7, after gray scale non-linear correction,
Standard curve it is linear good.Surveying originally water sample middle reaches from cl concn is 0.126mg/L, measurement result and uses dual wavelength point
The result that light photometer UV-1700 is measured matches.
Examples detailed above is used to illustrate the present invention, rather than limits the invention, in spirit and power of the invention
In the protection scope of force request, the modifications and changes that the present invention is made in any non-creative labour of those skilled in the art are both fallen within
In protection scope of the present invention.
Claims (10)
1. a kind of portable multi-channel spectrophotometer based on smart phone, which is characterized in that the spectrophotometer includes keeping away
Brightness box, light source, cell rack, measurement analytical unit, the measurement analytical unit is the smart phone for being provided with camera,
The cell rack is located between light source and measurement analytical unit, so that the camera face cuvette of measurement analytical unit
Frame；The light source and cell rack setting are being protected from light in magazine, and the measurement analytical unit setting is being protected from light outside magazine.
2. the portable multi-channel spectrophotometer according to claim 1 based on smart phone, which is characterized in that described
The magazine that is protected from light be provided with the first fixing clamp, be used for fixed light source.
3. the portable multi-channel spectrophotometer according to claim 1 based on smart phone, which is characterized in that described
The magazine that is protected from light be provided with the second fixing clamp, be used for fixation measuring analytical unit.
4. the portable multi-channel spectrophotometer according to claim 1 based on smart phone, which is characterized in that described
Cell rack more than two cuvette slots are arranged in parallel, for placing cuvette.
5. the portable multi-channel spectrophotometer according to claim 1 based on smart phone, which is characterized in that described
Cell rack in sample cell and reference cell are set, the sample cell and reference cell are cuvette；The sample cell
Quantity is two or more.
6. the portable multi-channel spectrophotometer according to claim 5 based on smart phone, which is characterized in that used
Cuvette is the standard items made of glass, absorbs light path are as follows: 10,20,30,50 or 100mm.
7. the portable multi-channel spectrophotometer according to claim 1 based on smart phone, which is characterized in that described
Cell rack and measure analytical unit camera between be additionally provided with wide-angle lens.
8. the portable multi-channel spectrophotometer according to claim 1 based on smart phone, which is characterized in that described
Light source be intelligence GRB virtually monochrome optical generator；
The intelligent GRB virtually monochromatic optical generator be preferably smart phone display screen, the display screen of smart phone opens
When, the light of sending is as light source.
9. the portable multi-channel spectrophotometer according to claim 8 based on smart phone, which is characterized in that in intelligence
Luminous zone can be set on mobile phone display wallpaper pattern, wallpaper pattern rest part is set as black, to reduce the shadow of backscatter light
It rings；Luminous zone is one group of rectangular patterns, fills corresponding with absorbing wavelength rgb value, the number of luminous zone, area, position and
Cuvette array in cell rack matches.
10. being inhaled using the described in any item portable multi-channel spectrophotometric determinations based on smart phone of claim 1-9
The method of luminosity, comprises the following steps that
(1) reference solution, sample solution are added separately to reference cell, in sample cell, are then placed on cell rack；
(2) the loading mobile phone wallpaper file on the smart phone display screen as light source, luminous zone face reference is molten on wallpaper
The position of liquid, sample solution, the rgb value of luminous zone color is corresponding with required absorbing wavelength, using the light of luminous zone as void
Quasi-simple color light source irradiates reference solution, sample solution simultaneously respectively；
(3) image for shooting reference solution, sample solution simultaneously with the camera of the smart phone of measurement analytical unit, calculates ginseng
Than solution, the gray value of sample solution image, absorbance A is calculated by formula (3)；
In formula (3), H0For the gray value of reference solution image, H is the gray value of sample solution image.
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| CN113310959A (en) * | 2021-05-25 | 2021-08-27 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Ascorbic acid detection method and detection preparation |
| CN113588630A (en) * | 2021-07-13 | 2021-11-02 | 曲靖师范学院 | Colorimetric method detector and detection method thereof |
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