CN109628573B - Kit for noninvasive prenatal detection of 12 chromosome microdeletion and microduplication syndrome and special probe set thereof - Google Patents

Abstract

The invention discloses a kit for noninvasive prenatal detection of 12 chromosome microdeletion microduplication syndromes and a special probe set thereof. The probe set comprises 600 specific probes. Each specific probe comprises a DNA fragment 1, a DNA fragment 2 and a DNA fragment 3 from a 5 'end to a 3' end in sequence. The nucleotide sequences of the DNA fragment 2 of the 600 specific probes are sequentially shown as 16 th to 93 th from the 5 'tail end of the sequence 1 in the sequence table to 16 th to 93 th from the 5' tail end of the sequence 600 in the sequence table. Experiments prove that the kit provided by the invention can detect whether the fetus to be detected has 12 chromosome microdeletion micro-repeat syndrome, and the detection result is completely consistent with the clinical SNP Aarray detection result. The invention has important application value.

Description

Kit for noninvasive prenatal detection of 12 chromosome microdeletion and microduplication syndrome and special probe set thereof

Technical Field

The invention belongs to the field of medicine, and particularly relates to a kit for noninvasive prenatal detection of 12 chromosome microdeletion and microduplication syndrome and a special probe set thereof.

Background

The chromosome microdeletion and microduplication syndrome refers to a genetic disease which is characterized in that a deleted or added segment on a chromosome is not very long, and chromosome aberration which is difficult to detect through traditional cytogenetic analysis causes complex clinical manifestations, and is another large group of birth defects of newborns besides chromosome aneuploidy. Common clinical manifestations of chromosomal microdeletion and microduplication are: some viscera abnormal development, abnormal growth and development, mental retardation, special face, abnormal endocrine, mental behavior change, etc. during the development process of the fetus.

For example, the Digeorge syndrome is a chromosome disease caused by deletion of a chromosome 22q11.2 segment, and is also called 22q11.2 microdeletion syndrome. The infant patients often show clinical phenotypes such as congenital heart disease, abnormal development of palate, gastrointestinal and genitourinary systems, immunodeficiency, developmental retardation, cognitive disorder and the like. Although a small proportion of Digeorge syndrome can be found by ultrasound, the vast majority will still be missed. One clinical study showed that one out of every 68 children with congenital heart disease had Digeorge syndrome. The incidence of the syndrome in neonates is 1/1000-1/4000, which is higher than that of trisomy 18 1/3500-1/7000.

At present, the detection technology aiming at the chromosome microdeletion and microreplication mainly comprises a karyotype analysis, a Fluorescence In Situ Hybridization (FISH) technology, a Multiple Ligation Probe Amplification (MLPA) technology, a Chromosome Microarray Analysis (CMA) technology and the like. Karyotyping is more limited because chromosome deletions and duplications below 5Mb are difficult to find. The FISH technique has higher sensitivity and specificity than the karyotype analysis, but can only be used in the diagnosis of specific target diseases or specific exclusion of certain diseases, and if the FISH technique is used as a screening technique of multiple chromosome microdeletion microduplication, the FISH technique is obviously insufficient, time-consuming, labor-consuming and high in cost. Chip technologies including SNP Array and Array CGH have good application to chromosome microdeletion microreplication, but the high detection cost becomes the clinical application's upper limit when the chip technology is also used for screening multiple chromosome microdeletion microreplication. More importantly, the three detection technologies are invasive, the detection sample needs amniotic fluid or chorion, and the abortion risk exists.

The high-throughput characteristic of the second generation sequencing technology is also very suitable for carrying out noninvasive prenatal gene detection. The direct sequencing of the whole genome has the problems of high cost, complex data analysis, long period and the like. The target sequence capture sequencing reduces the sequencing cost, improves the sequencing depth, and can more accurately find out the information of the sequence change of the undetermined area.

Disclosure of Invention

The invention aims to detect whether a fetus to be detected has 12 chromosome microdeletion and microduplication syndrome. The 12 chromosome microdeletion and microduplication syndrome is 1p36 deficiency syndrome, Cat's syndrome, peroneal muscular dystrophy, Digeorge syndrome, Duchen muscular dystrophy, Williams-Berren syndrome, Wolf-Hirschhorn syndrome, 15q13.3 microdeletion syndrome, Miller-Dieker syndrome, Smith-Magenis syndrome, Angel syndrome, Langer-Giediion syndrome.

The invention firstly protects a kit for detecting whether a fetus to be detected has the chromosome microdeletion micro-repeat syndrome shown in the following table, which can comprise a probe group;

the set of probes may comprise 600 specific probes; each specific probe may comprise DNA fragment 2; the DNA fragment 2 may be a part of a chromosome region corresponding to the chromosomal microdeletion and microduplication syndrome shown in the following table;

in the above kit, the DNA fragment 2 may be a single-stranded DNA molecule consisting of 60-140 (e.g., 60-78, 78-100, 100-140, 60, 78, 100 or 140) nucleotides.

In the kit, the nucleotide sequences of the DNA fragments 2 of the 600 specific probes can be sequentially shown as 16 th to 93 th from the 5 'tail end of the sequence 1 in the sequence table to 16 th to 93 th from the 5' tail end of the sequence 600 in the sequence table.

In the kit, each specific probe can also comprise a DNA fragment 1 and a DNA fragment 3; the DNA segment 1 is positioned at the 5' end of the DNA segment 2; the DNA segment 3 is located at the 3' end of the DNA segment 2.

The DNA segment 1 and the DNA segment 3 can be single-stranded DNA molecules consisting of 10-20 (such as 10-15, 15-20, 10, 15 or 20) nucleotides.

Each specific probe may include the DNA fragment 1, the DNA fragment 2 and the DNA fragment 3 in order from the 5 'end to the 3' end.

Any of the above probe sets may specifically consist of 600 specific probes. Each specific probe may specifically consist of the DNA fragment 1, the DNA fragment 2 and the DNA fragment 3 from the 5 'end to the 3' end.

Any one of the kits can also comprise a primer pair A; the primer pair A can consist of a primer 1 and a primer 2; the primer 1 may include the DNA fragment 1; the primer 2 may comprise the DNA fragment 3.

The primer 1 can be specifically the DNA fragment 1.

The primer 2 can be specifically the DNA fragment 3.

The primer 1 and/or the primer 2 can both have a biotin label.

Any one of the above-mentioned DNA fragments 1 and/or any one of the above-mentioned DNA fragments 3 has a biotin label (i.e., the biotin label can be directly performed on the above-mentioned DNA fragments 1 and/or the above-mentioned DNA fragments 3 without including a primer pair A in the kit).

The nucleotide sequence of any one of the DNA fragments 1 can be shown as a sequence 601 in a sequence table.

The nucleotide sequence of any one of the DNA fragments 3 can be shown as a sequence 602 in a sequence table.

The nucleotide sequences of any one of the 600 specific probes can be sequentially shown as a sequence 1 of a sequence table to a sequence 600 of the sequence table.

Any of the above kits may specifically consist of the set of probes.

Any one of the above kits may specifically consist of the probe set and the primer pair A.

Any one of the kits can also comprise a joint 1 (used for preparing a pregnant woman plasma free DNA library to be detected), a joint 2 (used for preparing a pregnant woman plasma free DNA library to be detected), a primer 3 (used for sealing the joint 1), a primer 4 (used for sealing the joint 2), a primer 5 (used for PCR amplification) and a primer 6 (used for PCR amplification);

the joint 1 comprises a DNA fragment a (namely a sequencing joint sequence 1), a DNA fragment b and a DNA fragment c from a 5 'end to a 3' end in sequence; the 3' end of the DNA fragment c can be T;

the joint 2 can comprise a DNA fragment d and a DNA fragment e from the 5 'end to the 3' end in sequence;

both the DNA fragment b and the DNA fragment d can be composed of A, T, C and G randomly;

the 5 'end of the DNA fragment e and the 3' end of the DNA fragment a are reversely complementary;

the primer 5 may include the DNA fragment a;

the primer 3 can comprise the DNA segment A, DNA segment B and the DNA segment C from the 5 'end to the 3' end in sequence; the 5' ends of the DNA fragment C and the DNA fragment a are completely consistent; the DNA fragment B can be composed of A, T, C and G randomly;

the primer 6 may include the DNA fragment D;

the primer 4 can comprise the DNA fragment D, DNA fragment E and the DNA fragment F from the 5 'end to the 3' end in sequence; the 3' ends of the DNA fragment F and the DNA fragment e are completely consistent; the DNA fragment E may be composed of A, T, C and G at random.

The DNA fragment b and the DNA fragment d may be the same or different.

The length of the DNA fragment c can be 1 bp.

The length of the DNA fragment b and the DNA fragment d is 6-14bp (such as 6-8bp, 8-14bp, 6bp, 8bp or 14 bp).

The length of the DNA fragment A is 17-25bp (such as 17-21bp, 21-25bp, 17bp, 21bp or 25 bp).

The length of the DNA fragment B is 1-8bp (such as 1-4bp, 4-8bp, 1bp, 4bp or 8 bp).

The length of the DNA fragment D is 17-25bp (such as 17-21bp, 21-25bp, 17bp, 21bp or 25 bp).

The length of the DNA fragment E is 10-25bp (such as 10-16bp, 16-25bp, 10bp, 16bp or 25 bp).

The primer 4 can also comprise a Barcode tag sequence; the Barcode tag sequence is located upstream, downstream or in the middle of the DNA fragment E (in one embodiment of the invention, the Barcode tag sequence is located in the middle of the DNA fragment E). The Barcode tag sequence has a length of 6-14bp (such as 6-8bp, 8-14bp, 6bp, 8bp or 14 bp). The Barcode labeling sequence can be composed of A, T, C and G randomly and is used for distinguishing different samples to be tested during sequencing data processing.

The 3' ends of the linker 1, the primer 5 and the primer 6 may all be thio modified.

The 5' end of the linker 2 may be modified for phosphorylation.

The linker 1 may specifically consist of a DNA fragment a (sequencing linker sequence 1), a DNA fragment b and a DNA fragment c from the 5 'end to the 3' end. In an embodiment of the invention, sequencing is performed using the illumina platform. Accordingly, the nucleotide sequence (from 5 'to 3') of the DNA fragment a (sequencing adapter sequence 1) may be ACACTCTTTCCCTACACGACGCTCTTCCGATCT. According to the difference of sequencing platform, the sequencing linker sequence composed of other nucleotide sequence can be replaced. The nucleotide sequence (from 5 'to 3') of the linker 1 can be specifically shown as a sequence 607 in a sequence table.

The linker 2 may specifically consist of a DNA fragment d and a DNA fragment e from the 5 'end to the 3' end. In an embodiment of the invention, sequencing is performed using the illumina platform. Accordingly, the nucleotide sequence (from 5 'to 3') of the DNA fragment e (sequencing adapter sequence 2) may be AGATCGGAAGAGCACACGTCT. According to the difference of sequencing platform, the sequencing linker sequence composed of other nucleotide sequence can be replaced. The nucleotide sequence (from 5 'to 3') of the linker 2 can be specifically shown as a sequence 608 in the sequence table.

The primer 3 may specifically consist of the DNA fragment A, the DNA fragment B and the DNA fragment C from the 5 'end to the 3' end. The nucleotide sequence (from 5 'to 3') of the primer 3 can be specifically shown as a sequence 603 in a sequence table.

The primer 4 may specifically consist of the DNA fragment D, the DNA fragment E (containing the Barcode tag sequence) and the DNA fragment F from the 5 'end to the 3' end. The nucleotide sequence (from 5 'to 3') of the primer 4 can be specifically shown as a sequence 604 in a sequence table.

The primer 5 may specifically consist of the DNA fragment A. The nucleotide sequence (from 5 'to 3') of the primer 5 can be specifically shown as a sequence 605 in a sequence table.

The primer 6 may specifically consist of the DNA fragment D. The nucleotide sequence (from 5 'to 3') of the primer 5 can be specifically shown as a sequence 606 in a sequence table.

Any one of the above kits may specifically consist of the probe set, the linker 1, the linker 2, the primer 3, the primer 4, the primer 5, and the primer 6.

Any one of the above kits may specifically consist of the probe set, the primer pair a, the linker 1, the linker 2, the primer 3, the primer 4, the primer 5, and the primer 6.

Any of the above kits may further comprise a carrier carrying a judgment standard.

The judgment criteria may be as follows: firstly, according to a formula (1), measuring the Z-score value of a certain area of a pregnant woman sample;

z-score: the Z-score value of a certain area of a pregnant woman sample to be detected;

readssample: the areas of the pregnant woman samples to be detected are normalized reads;

mean reads reference: referring to the average value of the area reads in the database;

(s.d.reads reference: the standard deviation of reads of the reference database;

then, the following judgment is made: if the Z-score value of a certain area of the pregnant woman sample to be detected is larger than 3, the area is repeated; if the Z-score value of a certain area of the pregnant woman sample to be detected is less than or equal to 3, the area is normal; if the z-score value of a certain area of the pregnant woman sample to be detected is less than-3, the area is absent; if a region is duplicated or deleted, the fetus suffers from the corresponding chromosome microdeletion and microduplication syndrome; if a region is normal, the fetus does not have the corresponding chromosome microdeletion microreplication syndrome.

The reference database may be composed of data of M healthy pregnant women together; m is a natural number of 100 or more. Dividing the reference database into regions, calculating the number of reads of each region, and homogenizing samples based on the median of the reads of each region; and then calculating the average value and the standard deviation of each region ready in the reference database, namely respectively obtaining the average value of the region ready in the reference database and the standard deviation of the region ready in the reference database.

The steps of obtaining data for each healthy pregnant woman may be as follows:

(K1) preparing a plasma free DNA library of a healthy pregnant woman; the linkers for preparing the plasma free DNA library of healthy pregnant women may be the linker 1 and the linker 2;

(K2) after the step (K1) is completed, hybridizing the healthy pregnant woman plasma free DNA library with the probe set to obtain a hybridization product;

(K3) after the step (K2) is completed, collecting the target sequence from the hybridization product to obtain a target sequence capture library;

(K4) performing high-throughput sequencing on the target sequence capture library obtained in the step (K3) to obtain sequencing original data;

(K5) cutting off an adaptor sequence and low-quality bases from the sequencing original data obtained in the step (K4), filtering (if the filtering length is less than 40bp sequence), and then aligning to the corresponding position of the hg19 of the reference genome;

(K6) after completion of step (K5), the repetitive sequences due to PCR amplification preference were removed, and data of the healthy pregnant woman were obtained.

In an embodiment of the present invention, any of the above reference databases may specifically consist of data of 400 healthy pregnant women delivering healthy fetuses.

Any one of the above kits may specifically comprise the probe set, the linker 1, the linker 2, the primer 3, the primer 4, the primer 5, the primer 6, and the vector on which the judgment standard is recorded.

Any one of the above kits may specifically comprise the probe set, the primer pair a, the linker 1, the linker 2, the primer 3, the primer 4, the primer 5, the primer 6, and the vector on which a judgment standard is recorded.

Any of the kits described above may further comprise conventional reagents for performing PCR amplification.

Any of the above kits may further comprise conventional reagents for hybridization of the DNA library to the probe.

The invention also provides a method for detecting whether a chromosome region corresponding to the chromosome microdeletion and microduplication syndrome of a fetus to be detected is deleted, duplicated or normal, which sequentially comprises the following steps:

(1) preparing a pregnant woman plasma free DNA library to be detected or a pregnant woman genome DNA library to be detected;

(2) after the step (1) is finished, hybridizing the pregnant woman plasma free DNA library to be detected or the pregnant woman genome DNA library to be detected with any probe set to obtain a hybridization product;

(3) after the step (2) is completed, collecting the target sequence from the hybridization product to obtain a target sequence capture library;

(4) and (4) after the step (3) is completed, sequencing the target sequence capture library, and judging whether a chromosome region corresponding to a certain chromosome microdeletion and microduplication syndrome of the fetus to be detected is deleted, duplicated or normal according to sequencing data.

In the step (1), the linker for preparing the isolated DNA library of plasma of the pregnant woman to be tested or the genomic DNA library of the pregnant woman to be tested can be any one of the above-mentioned linker 1 and linker 2.

The steps for preparing the pregnant woman plasma free DNA library to be detected can specifically be as follows: firstly, extracting the plasma free DNA of the pregnant woman to be detected, and then preparing the plasma free DNA library of the pregnant woman to be detected by utilizing a KAPA Hyper library construction kit (the joint can be any one of the joint 1 and the joint 2).

The steps for preparing the pregnant woman genome DNA library to be detected can specifically be as follows: firstly, extracting the genomic DNA of the pregnant woman to be detected, then breaking the genomic DNA to about 200bp by ultrasonic, and finally preparing the genomic DNA library of the pregnant woman to be detected by utilizing a KAPA Hyper library construction kit.

Any one of the above KAPA Hyper library construction kits may be specifically a product of KAPA Biosystems, cat # KK 8504.

In the step (2), "hybridizing the isolated DNA library of plasma of the pregnant woman to be tested or the genomic DNA library of the pregnant woman to be tested with the probe set to obtain a hybridization product" may be as follows:

(2-1) preparing an enrichment system; the enrichment system comprises the pregnant woman plasma free DNA library to be detected or the pregnant woman genome DNA library to be detected, the probe group, the primer 3 and the primer 4;

(2-2) taking the enrichment system, and hybridizing and enriching for more than 16h (such as 16h) at 60-70 ℃ (such as 60-65 ℃, 65-70 ℃, 60 ℃, 65 ℃ or 70 ℃) to obtain a hybridization product.

In the step (2-1), the enrichment system can be composed of a pregnant woman plasma free DNA library to be detected or a pregnant woman genome DNA library to be detected, human cot-1DNA, salmon sperm DNA, the primer 3, the primer 4, a probe set and a hybridization buffer (preheated at 65 ℃).

In the step (2-1), 100 μ L of the enrichment system can be composed of 48 μ L of pregnant woman plasma free DNA library to be detected (containing 1 μ g of pregnant woman plasma free DNA to be detected) or pregnant woman genome DNA library to be detected (containing 1 μ g of pregnant woman genome DNA to be detected), 12 μ L of enrichment buffer, 5 μ L of probe set and 35 μ L of hybridization buffer (preheated at 65 ℃). The enrichment buffer may consist of 3.5 parts by volume of human cot-1DNA aqueous solution, 3.5 parts by volume of salmon sperm DNA aqueous solution, and 3 parts by volume of primer mixture (consisting of primer 3, primer 4 and water). In the enrichment buffer, the concentration of human cot-1DNA may be 30% to 45% (e.g., 30% to 35%, 35% to 45%, 30%, 35%, 40% or 45%) (v/v), the concentration of salmon sperm DNA may be 5% to 25% (e.g., 5% to 15%, 15% to 25%, 5%, 10%, 15%, 20% or 25%), the concentration of primer 3 may be 0.2 to 1nmol/μ L (e.g., 0.2 to 0.5nmol/μ L, 0.5 to 1nmol/μ L, 0.2nmol/μ L, 0.3nmol/μ L, 0.5nmol/μ L, 0.6nmol/μ L or 1nmol/μ L), and the concentration of primer 4 may be 0.2 to 1nmol/μ L (e.g., 0.2 to 0.5nmol/μ L, 0.5 to 1nmol/μ L, 0.2nmol/μ L, 0.3nmol/μ L, or μ L), 0.5 nmol/. mu.L, 0.6 nmol/. mu.L, or 1 nmol/. mu.L).

The hybridization buffer may be an aqueous solution containing 1.0-1.5M (e.g., 1.0-1.25M, 1.25-1.5M, 1M, 1.25M, or 1.5M) NaCl, 0.1-0.3M (e.g., 0.1-0.15M, 0.15-0.3M, 0.1M, 0.125M, 0.15M, 0.2M, or 0.3M) sodium citrate, 0.08-0.12g/100mL (e.g., 0.08-0.10g/100mL, 0.10-0.12g/100mL, 0.08g/100mL, 0.10g/100mL, or 0.12g/100mL), and 5-9% (e.g., 5-7%, 7-9%, 5%, 6%, 7%, 8%, or 9%) (v/v) BSA 20.

In the step (2-2), the hybridization may be performed in a PCR apparatus. The reaction procedure may be: 7min at 95 ℃ and more than 16h at 65 ℃.

In the step (3), "collecting target sequence from hybridization product and obtaining target sequence capture library" may be as follows:

(3-a) collecting the hybridization product using magnetic beads;

and (3-b) performing PCR amplification by using the product collected in the step (3-a) as a template and adopting a primer pair consisting of the primer 5 and the primer 6, wherein the PCR amplification product is a target sequence capture library.

The step (3-a) may specifically include the following step (3-1), step (3-2), step (3-3) and step (3-4).

The step (3-b) may specifically include the following steps (3-5), (3-6) and (3-7).

In the step (3), "collecting target sequence from hybridization product and obtaining target sequence capture library" may be as follows:

(3-1) preparing a solution of streptavidin-coated magnetic beads;

(3-2) adding the solution of the streptavidin-coated magnetic beads and the hybridization product into a centrifuge tube, and incubating; then placing the mixture in a magnetic frame or centrifuging, and discarding the supernatant;

(3-3) after the step (3-2) is finished, taking the centrifugal tube, adding a rinsing buffer solution into the centrifugal tube, and washing;

(3-4) after the step (3-3) is finished, taking the centrifuge tube and resuspending the magnetic beads; then placing the mixture in a magnetic frame or centrifuging, and collecting the supernatant;

(3-5) preparing a PCR amplification system; the PCR amplification system comprises the supernatant collected in the step (3-4), the primer 5 and the primer 6 in the claims 7 to 10;

(3-6) taking the PCR amplification system, and carrying out PCR amplification to obtain a PCR amplification product;

(3-7) taking the PCR amplification product, and purifying to obtain a target sequence, namely a target sequence capture library.

In the step (3-2), the incubation may be performed at room temperature for 0.5-2h (e.g., 0.5-1h, 1-2h, 0.5h, 1h or 2 h). The magnetic frame can be placed for 1 min.

In the step (3-3), the "adding a rinsing buffer solution and washing" can be performed in two steps: adding a rinsing buffer solution 1 for washing; the second step was washing with addition of rinse buffer 2. The purpose is to wash away the specific probes that are not bound to the streptavidin magnetic beads.

The rinsing buffer 1 may be a sodium citrate buffer containing 0.05-0.2% (m/v) SDS (e.g., a sodium citrate buffer containing 0.05-0.1% (m/v) SDS, a sodium citrate buffer containing 0.1-0.2% (m/v) SDS, a sodium citrate buffer containing 0.05% (m/v) SDS, a sodium citrate buffer containing 0.1% (m/v) SDS, or a sodium citrate buffer containing 0.2% (m/v) SDS). The sodium citrate buffer solution can be an aqueous solution containing 175g/L NaCl and 88g/L trisodium citrate; the pH was 7.4.

The rinsing buffer 2 may specifically be a dilution of sodium citrate buffer containing 0.05 to 0.2% (m/v) SDS (e.g., a dilution of sodium citrate buffer containing 0.05 to 0.1% (m/v) SDS, a dilution of sodium citrate buffer containing 0.1 to 0.2% (m/v) SDS, a dilution of sodium citrate buffer containing 0.05% (m/v) SDS, a dilution of sodium citrate buffer containing 0.1% (m/v) SDS, or a dilution of sodium citrate buffer containing 0.2% (m/v) SDS). The sodium citrate buffer diluent can be prepared by mixing 1 volume part of sodium citrate buffer and 9 volume parts of water.

In step (3-4), "resuspended beads" are resuspended by adding a library enrichment eluent. The library enrichment eluent may be an aqueous NaOH solution with a concentration of 0.1M.

In the step (3-5), the PCR amplification system may consist of the supernatant collected in the step (3-4), a neutralization buffer and a PCR reaction solution.

The neutralization buffer solution can be specifically Tris-HCl buffer solution with the pH value of 7.5 and the concentration of 1M.

The PCR reaction solution consists of Phusion Hot Start II DNA Polymerase, dNTP, DMSO, primer 5, primer 6 and MgCl ₂ KCl, Nonidet P40 and water.

A3 mL PCR reaction solution may specifically consist of 100. mu.L of Phusion Hot Start II DNA Polymerase (product of thermo company, cat # F549S) and 2900. mu.L of PCR mixbuffer. PCR mixbuffer containing 0.2mM dATP, 0.2mM dTTP, 0.2mM dCTP, 0.2mM dGTP, 5% (v/v) DMSO, 2.5pmol primer 5, 2.5pmol primer 6, 4mM MgCl ₂ 500mM KCl and 0.8% (v/v) Nonidet P40.

In the step (3-6), the procedure of "performing PCR amplification" may be: 30sec at 98 ℃; 30sec at 98 ℃, 30sec at 65 ℃, 30sec at 72 ℃, 15 cycles; 72 ℃ for 5min, 4 ℃ infinity.

In the step (3-7), the purification may be performed by using an Agencourt AMPure XP nucleic acid purification kit (product of Beckman Coulter, product number a 63881).

In the step (4), "sequencing the target sequence capture library, and judging whether a chromosome region corresponding to a certain chromosome microdeletion and microduplication syndrome of a fetus to be detected is missing, duplicated or normal according to sequencing data" may be to sequence the target sequence capture library to obtain sequencing original data; processing sequencing original data to obtain sequencing data; obtaining normalized reads of a certain area of the pregnant woman to be detected according to the sequencing data; and judging whether the chromosome region corresponding to the chromosome microdeletion and microduplication syndrome of the fetus to be detected is deleted, duplicated or normal according to the normalized reads in a certain region of the pregnant woman to be detected.

The processing of the sequencing raw data comprises the following steps:

(4-1) cutting off the adaptor sequence and low-quality base from the sequencing original data, filtering (the filtering length is less than 40bp sequence), and aligning to the corresponding position of the hg19 of the reference genome;

(4-2) after completion of step (4-1), removing repetitive sequences due to PCR amplification bias to obtain sequencing data.

In the step (4), the criterion for determining whether the chromosome region corresponding to the microdeletion and microduplication syndrome of a chromosome of the fetus to be detected is missing, duplicated or normal according to the sequencing data may be any of the above-mentioned criteria.

The invention also provides a target sequence capture library, which consists of the target sequences obtained by any one of the methods.

The invention also protects any probe set.

The invention also protects the application of any probe set, which can be W1) or W2):

w1) detecting whether the chromosome region corresponding to the chromosome microdeletion and microduplication syndrome of the fetus to be detected is deleted, duplicated or normal;

w2) detecting whether the fetus to be detected has the chromosome microdeletion and microduplication syndrome.

The platform for any of the above described sequencing may be Nextseq500, X Ten or NovaSeq.

Any of the above-described chromosomal regions is a chromosomal location of the chromosomal microdeletion and microduplication syndrome (e.g., as indicated by the base position above).

Experiments prove that the kit provided by the invention can detect whether the fetus to be detected has the 12-chromosome microdeletion and microduplication syndrome. The 12 chromosome microdeletion and microdroplet syndrome is respectively 1p36 deletion syndrome, crinis felis syndrome, peroneal muscular dystrophy, Digeorge syndrome, Duchen muscular dystrophy, Williams-Beruren syndrome, Wolf-Hirschhorn syndrome, 15q13.3 microdeletion syndrome, Miller-Dieker syndrome, Smith-Magenis syndrome, Angel syndrome and Langer-Giedion syndrome, and the detection result is completely consistent with the detection result of clinical SNP Aarray. The invention has important application value.

Drawings

FIG. 1 shows the detection result of a peripheral blood sample of a high-risk pregnant woman with Williams-Berren syndrome detected by SNP Aarray in clinic.

FIG. 2 is a detection result of a peripheral blood sample of a pregnant woman at high risk of Wolf-Hirschhorn syndrome detected by SNP Aarray in clinic.

FIG. 3 is a detection result of a peripheral blood sample of a pregnant woman at high risk of Smith-magenta syndrome detected clinically by SNP Aarrray.

FIG. 4 is a detection result of a peripheral blood sample of a pregnant woman at high risk of Miller-Dieker syndrome detected by SNP Aarray in clinic.

Fig. 5 is a detection result of a peripheral blood sample of a pregnant woman at high risk of peroneal muscular atrophy detected by SNP Aarray in clinic.

Detailed Description

The following examples are given to facilitate a better understanding of the invention, but do not limit the invention.

The experimental procedures in the following examples are all conventional ones unless otherwise specified.

The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.

The pregnant women in the following examples gave their informed consent to the experimental contents.

The names, locations and regions of the 12 chromosomal microdeletion microduplication syndromes referred to in the examples below are detailed in table 1.

TABLE 1

Name of chromosome microdeletion microreplication syndrome	Located chromosome	Corresponding chromosomal region
			1p36 deficiency syndrome	Chr1	10001-12840259
Cat cry syndrome	Chr5	10001-12533304
			Peroneal muscular atrophy	Chr17	14097915-15470903
Digeorge syndrome	Chr22	19009792-21452445
			Duchenne muscular dystrophy	ChrX	31135345-33231673
Williams-Berren syndrome	Chr7	72744455-74142672
			Wolf-Hirschhorn syndrome	Chr4	492989-1983934
15q13.3 microdeletion syndrome	Chr15	30910306-32445407
			Miller-Dieker syndrome	Chr17	1-2588909
Smith-Magenis syndrome	Chr17	16773072-20222149
			Syndrome of angel's foot	Chr15	22749354-28438266
Langer-Giedion syndrome	Chr8	108776506-121312414

The nucleotide sequences of the primers referred to in the examples below are shown in Table 2.

TABLE 2

Note: ^* g represents that G is subjected to a thio modification, ^* a represents that A is subjected to thio modification, ^* t is T for thio modification, P is phosphorylation modification, and N is A, T, C or G.

Example 1 preparation of a kit for noninvasive prenatal detection of 12 chromosome microdeletion microduplication syndrome

First, preparation of Probe set

1. 600 probes were designed and synthesized based on the chromosomal region corresponding to the 12 chromosomal microdeletion and microduplication syndrome (shown in column 3 of Table 1). Each probe consists of 108 nucleotides, and comprises a DNA fragment 1, a DNA fragment 2 and a DNA fragment 3 from a 5 'end to a 3' end in sequence. The nucleotide sequence of the DNA fragment 1 is 5'-GACTACATGGGACAT-3'. The nucleotide sequence of the DNA fragment 3 is 5'-GGAACCTACGACGTA-3'. DNA fragment 2 consisted of 78 nucleotides. The DNA fragment 2 is a part of the chromosome region corresponding to 12 chromosome microdeletion and microduplication syndrome.

2. After step 1 was completed, the probe was biotin-labeled. The method comprises the following specific steps: 600 probes were mixed well in ddH with a total volume of 1.2mL ₂ In O, 15. mu.L of the mixture was subjected to PCR amplification (performed in three tubes) using a universal PCR primer set consisting of a primer 1:5 ' -bio-GACTACATGGGACAT-3 ' (bio indicates that the 5 ' end has a biotin label) and a primer 2: 5'-GGAACCTACGACGTA-3' to obtain a PCR amplification product.

3. Purifying the PCR amplification product with MinElute PCR Purification Kit (product of Qiagen, cat # 28006) to obtain a purified product; then 500ng of the purified product was taken and bound with MyOne streptavidin magnetic beads (product of Invitrogen, cat # 35602); then adding NaOH solution for treatment, aiming at denaturing and eluting the complementary strand without biotin labeling; then washing the whole magnetic beads with formamide liquid at 100 ℃ to separate the probes from the magnetic beads; and finally, precipitating with ethanol to obtain the biotin-labeled probe set.

Preparation of kit for noninvasive prenatal detection of 12 chromosome microdeletion and microduplication syndrome

The kit for noninvasive prenatal detection of 12 chromosome microdeletion microduplication syndromes consists of enrichment buffer, hybridization buffer, library combination buffer, rinsing buffer 1, rinsing buffer 2, library enrichment eluent, neutralization buffer, PCR reaction liquid and a probe set prepared in the first step.

The enrichment buffer solution consists of 3.5 volume parts of human cot-1DNA aqueous solution, 3.5 volume parts of salmon sperm DNA aqueous solution and 3 volume parts of primer mixture solution (consisting of a primer 3, a primer 4 and water). In the enrichment buffer, the concentration of human cot-1DNA was 35% (v/v), the concentration of salmon sperm DNA was 15% (v/v), the concentration of primer 3 was 0.5 nmol/. mu.L, and the concentration of primer 4 was 0.5 nmol/. mu.L.

Hybridization buffer: an aqueous solution containing 1.25M NaCl, 0.125M sodium citrate, 0.1g/100mL BSA and 7% (v/v) Tween 20.

Library binding buffer: 10mM Tris-HCl buffer, pH7.5, containing 1M NaCl and 1mM EDTA.

Rinsing buffer 1: sodium citrate buffer containing 0.1% (m/v) SDS. The sodium citrate buffer solution is an aqueous solution containing 175g/L NaCl and 88g/L trisodium citrate; the pH was 7.4.

Rinsing buffer 2: dilutions of sodium citrate buffer containing 0.1% (m/v) SDS. The sodium citrate buffer solution diluent is formed by mixing 1 volume part of sodium citrate buffer solution and 9 volume parts of water.

Library enrichment eluent: aqueous NaOH solution having a concentration of 0.1M.

Neutralization buffer: Tris-HCl buffer (pH 7.5, 1M).

3mL of the PCR reaction solution consisted of 100. mu.L of Phusion Hot Start II DNA Polymerase (product of Thermo, cat # F549S) at a concentration of 0.05U/. mu.L and 2900. mu.L of PCR mixbuffer. PCR mixbuffer was an aqueous solution containing 0.2mM dATP, 0.2mM dTTP, 0.2mM dCTP, 0.2mM dGTP, 5% (v/v) DMSO, 2.5pmol primer 5, 2.5pmol primer 6, 4mM MgCl2, 500mM KCl, and 0.8% (v/v) Nonidet P40.

Example 2 and example 1 establishment of a method for noninvasive prenatal detection of whether a fetus has a chromosome microdeletion and microduplication syndrome by using the kit prepared in example 2

Through a large number of experiments, the inventor establishes a method for noninvasive prenatal detection of whether a fetus has the chromosome microdeletion and microduplication syndrome by using the kit prepared in the embodiment 1. The method comprises the following specific steps:

extraction of free DNA in blood plasma of pregnant woman to be detected

1. Collecting 10mL of peripheral blood of a pregnant woman to be detected by using an EDTA anticoagulant tube, centrifuging for 15min at 4 ℃ at 1600g, and collecting supernatant 1 (placing in a low-adsorption centrifuge tube).

2. After completion of step 1, the supernatant 1 was centrifuged at 12000rpm for 5min and the supernatant 2 was collected (placed in a low adsorption centrifuge tube). Supernatant 2 is plasma.

3. After completion of step 2, 1mL of the supernatant 2 was taken, and plasma free DNA (cfDNA) was extracted using a large-volume free nucleic acid extraction kit (product of Tiangen Biochemical technology (Beijing) Ltd., product No. DP 710). The cfDNA is named as the plasma free DNA of the pregnant woman to be detected.

Preparation of pregnant woman plasma free DNA library to be detected

And (3) taking 5ng of the pregnant woman plasma free DNA to be detected obtained in the step one, and preparing the pregnant woman plasma free DNA library to be detected by using a KAPA Hyper library construction kit (a product of KAPA Biosystems, the product number is KK 8504). The joints for preparing the pregnant woman plasma free DNA library to be detected are single-stranded DNA1 and single-stranded DNA 2.

Taking a pregnant woman plasma free DNA library to be detected, and carrying out Qubit quantification and 2% agarose gel electrophoresis detection.

Thirdly, extracting the genome DNA of the pregnant woman to be detected

2mL of peripheral Blood of a pregnant woman to be tested is collected by an EDTA anticoagulant tube, and genome DNA is extracted by a CWE2100Blood DNA Kit V2 (Kang is a product of century Biotechnology Co., Ltd., product number CW 2543). The genomic DNA is named as the genomic DNA of the pregnant woman to be detected.

Fourth, preparation of pregnant woman genome DNA library to be tested

3 mu g of pregnant woman genome DNA to be detected is taken, ultrasonic breaking (the instrument model is Covaris S220, the parameters are Peak inclusion Power 175W, Duty Factor 10%, Cycles per Burst 200 and Treatment Time 180S) is carried out to about 200bp, and then a KAPA Hyper library construction kit is utilized to prepare the pregnant woman genome DNA library to be detected. The joints for preparing the genome DNA library of the pregnant woman to be detected are single-stranded DNA1 and single-stranded DNA 2.

Taking a genome DNA library of the pregnant woman to be detected, and carrying out Qubit quantification and 2% agarose gel electrophoresis detection.

Fifth, construction of target sequence Capture library

1. And (4) preparing an enrichment system. The enrichment system is 100 mu L and consists of 48 mu L of a DNA library to be detected (a pregnant woman plasma free DNA library to be detected (containing 1 mu g of pregnant woman plasma free DNA to be detected) or a pregnant woman genome DNA library to be detected (containing 1 mu g of pregnant woman genome DNA to be detected)), 12 mu L of enrichment buffer solution, 5 mu L of probe set and 35 mu L of hybridization buffer solution (preheated at 65 ℃).

2. And (3) after the step 1 is finished, taking the enrichment system, and putting the enrichment system in a PCR instrument for overnight hybridization enrichment (the hybridization time is at least 16h) to obtain an enrichment product.

Reaction procedure: 7min at 95 ℃ and more than 16h at 65 ℃.

3. Obtaining solution of streptavidin-coated magnetic beads (streptavidin-coated magnetic beads are stored in a storage solution, which is performed to remove the storage solution)

(1) Adding 50 μ L of the low-adsorption centrifuge tube

M-280 streptavidin magnetic beads, then placed in the magnetic frame for 1 min.

(2) After completion of step (1), the supernatant was discarded, 50. mu.L of library binding buffer was added, the beads were resuspended, and then briefly centrifuged.

(3) And (3) after the step (2) is finished, taking the low-adsorption centrifuge tube, placing the low-adsorption centrifuge tube in a magnetic frame for 1min, discarding the supernatant, adding 50 mu L of library binding buffer solution, and resuspending the magnetic beads.

(4) And (4) after the step (3) is finished, taking the low-adsorption centrifuge tube, placing the low-adsorption centrifuge tube in a magnetic frame for 1min, discarding the supernatant, then adding 50 mu L of library combination buffer solution, and re-suspending the magnetic beads to obtain a solution of streptavidin coated magnetic beads.

4. Co-incubation capture

(1) And (3) after the step 3 is finished, adding all the enriched products obtained in the step 2 into the low-adsorption centrifuge tube, performing vortex oscillation for 5sec, and then placing the tube on a rotary blending machine at room temperature for 1 h.

(2) And (3) after the step (1) is finished, taking the low-adsorption centrifuge tube, placing the low-adsorption centrifuge tube in a magnetic frame for 1min, and discarding the supernatant.

5. Washing and PCR amplification

(1) And (4) after the step 4 is finished, adding 500 mu L of rinsing buffer solution 1 into the low-adsorption centrifuge tube, uniformly mixing, and placing the mixture in a rotary mixer to rotate for 15 min.

(2) And (3) after the step (1) is finished, taking the low-adsorption centrifuge tube, placing the centrifuge tube on a magnetic frame for standing for 2min, and discarding the supernatant.

(3) After step (2) was completed, the low adsorption centrifuge tube was added with 500. mu.L of rinsing buffer 2 (preheated at 65 ℃) and incubated at 65 ℃ for 10 min.

(4) And (4) after the step (3) is finished, taking the low-adsorption centrifuge tube, placing the centrifuge tube on a magnetic frame, standing for 2min, and discarding the supernatant.

(5) And (4) repeating the step (4) twice.

The purpose of carrying out the above steps is to not react with

The probes bound to the M-280 streptavidin magnetic beads were washed away.

(6) And (5) after the step (5) is finished, adding 30 mu L of library enrichment eluent into the low-adsorption centrifuge tube, resuspending magnetic beads, and placing the low-adsorption centrifuge tube in a room-temperature rotary mixer for 10-20 min.

(7) And (5) after the step (6) is finished, taking the low-adsorption centrifuge tube, and placing the centrifuge tube on a magnetic frame for standing for 1 min.

(8) And (5) after the step (7) is finished, transferring the supernatant to a centrifuge tube, adding 40 mu L of neutralization buffer solution and 30 mu L of PCR reaction solution, sucking by a pipette, mixing uniformly, and placing in a PCR instrument for PCR amplification to obtain a PCR amplification product.

PCR procedure: 30sec at 98 ℃; 30sec at 98 ℃, 30sec at 65 ℃, 30sec at 72 ℃, 15 cycles; 72 ℃ for 5min, 4 ℃ infinity.

6. Purification of

After the step 5 is completed, the PCR amplification product is taken and purified by an Agencourt AMPure XP nucleic acid purification kit (product of Beckman Coulter company, the product number is A63881), and a library amplification purification product is obtained.

The library amplification purification product is the target sequence capture library.

Sixth, high throughput sequencing and data analysis

1. And (4) carrying out high-throughput sequencing on the target sequence capture library obtained in the fifth step through a second-generation sequencing platform such as Nextseq500, X Ten, Novaseq and the like to obtain sequencing original data.

2. After completion of step 1, the sequencing raw data were cut out of linker sequences, low quality bases (quality value less than 10), filtered for sequences less than 40bp in length using cutdata software (https:// cutdata. readthe docs. io/en/stable /), and then aligned to the corresponding position of the reference genome hg19 by bwa software (http:// bio-bw. sourceform. net /) or bowtie software (http:// http:// bowtie-bio. sourceform. net/bowtie2 /).

3. After the step 2 is completed, the biobamam 2 software (https:// software. broadinstruction. org/gatk /) is used for removing the repeated sequences caused by the PCR amplification preference, and the data of the pregnant woman sample to be detected are obtained.

4. Establishment of a reference database

(1) Plasma free DNA extraction of the subject

Replacing the pregnant woman to be detected in the step one with a certain person to be detected, and obtaining the plasma free DNA of the person to be detected, wherein other steps are unchanged.

(2) Preparation of a test sample plasma free DNA library

And (2) respectively taking 5ng of plasma free DNA of the testee obtained in the step (1), and preparing the plasma free DNA library of the testee by utilizing a KAPA Hyper library construction kit. The joints for preparing the pregnant woman plasma free DNA library to be detected are single-stranded DNA1 and single-stranded DNA 2.

(3) Construction of target sequence Capture libraries

Replacing the 'pregnant woman plasma free DNA library to be detected' in the third step with the blood plasma free DNA library to be detected prepared in the step (2) respectively, and obtaining a target sequence capture library without changing other steps.

(4) And (4) carrying out high-throughput sequencing on the target sequence capture library obtained in the step (3) through a second-generation sequencing platform such as Nextseq500, X Ten, Novaseq and the like to obtain sequencing original data.

(5) And (3) cutting the joint sequence and the low-quality base (the quality value is less than 10) of the sequencing raw data obtained in the step (4) by using cutadapt software, filtering the sequence with the length of less than 40bp, and then aligning the sequence to the corresponding position of hg19 of a reference genome by using bwa software or bowtie software.

(6) After step (5) is completed, the repetitive sequences caused by the PCR amplification preference are removed by using biobamam 2 software, and the data of the testee are obtained.

The subjects to be tested were 400 normal pregnant women (i.e., healthy pregnant women who delivered healthy fetuses). The data of 400 testees together form a reference database.

5. Dividing the reference database into regions (including non-capture regions) by using cnvkit software (the website is https:// cnvkit: readthetadocs. io/en/stable /), calculating the number of reads in each region, and making sample homogenization based on the median of the reads in each region; the Mean (Mean) and Standard Deviation (SD) of the reads for each region in the reference database are then calculated.

6. After the step 5 is completed, calculating the z-score value of a certain area of the pregnant woman sample to be detected according to the following formula, and then judging as follows: if the z-score value of a certain area of the pregnant woman sample to be detected is larger than 3, the area is repeated; if the z-score value of a certain area of the pregnant woman sample to be detected is less than-3, the area is absent.

Z-score: the z-score value of a certain area of the pregnant woman sample to be detected;

readssample: obtaining the normalized reads of the area of the pregnant woman sample to be detected according to the pregnant woman sample data to be detected obtained in the step 4;

mean reads reference: referring to the average value of the reads of the region in the database;

(s.d.reads reference: the standard deviation of the area reads in the database is referenced.

Example 3 noninvasive prenatal determination of whether a fetus has a chromosomal microdeletion and microduplication syndrome using the kit prepared in example 1

The sample to be detected is 1 peripheral blood sample of a pregnant woman with high risk of Williams-Beuren syndrome through clinical SNP Aarry detection (numbered 1), 1 peripheral blood sample of a pregnant woman with high risk of Wolf-Hirschhorn syndrome through clinical SNP Aarry detection (numbered 2), 1 peripheral blood sample of a pregnant woman with high risk of Smith-Magenis syndrome through clinical SNP Aarry detection (numbered 3), 1 peripheral blood sample of a pregnant woman with high risk of Miller-Dieker syndrome through clinical SNP Aarry detection (numbered 4), 1 peripheral blood sample of a pregnant woman with high risk of peroneal muscular atrophy through clinical SNP Aarry detection (numbered 5), 1 peripheral blood sample of a pregnant woman with high risk of deficiency syndrome through 1p36 through SNP Aarrray detection (numbered 6), 1 peripheral blood sample of a high risk of called syndrome through clinical SNP Aarrage detection (numbered 7), and 1 peripheral blood sample of a Dieoryegray detection of a peripheral blood syndrome through SNP Aarry detection (numbered 8), The kit comprises 1 peripheral blood sample (numbered 9) of a Duchenne muscular dystrophy high-risk pregnant woman detected by an SNP Aarray in clinical cases, 1 peripheral blood sample (numbered 10) of a high-risk pregnant woman with 15q13.3 microdeletion syndrome detected by an SNP Aarray in clinical cases, 1 peripheral blood sample (numbered 11) of a high-risk pregnant woman with angelan syndrome in clinical cases and 1 peripheral blood sample (numbered 12) of a Langer-Giedion syndrome detected by an SNP Aarray in clinical cases.

And taking a sample to be detected, and detecting according to the method established in the embodiment 2. The basic data for a portion of the samples tested are shown in Table 3.

TABLE 3

Numbering	Mean depth of sequencing	Capture efficiency (%)	Depth>＝20X(％)
				1	374.46	31.83	94.52
2	370.07	33.64	94.74
				3	395.79	37.49	96.4
4	364.57	34.28	94.44
				5	395.02	34.72	94.71

And drawing a dot diagram by taking the number of divided areas based on the cnvkit software as an abscissa and the z-score value of each area of each sample as an ordinate. The detection results of a part of the samples to be detected are shown in fig. 1 (sample to be detected with number 1), fig. 2 (sample to be detected with number 2), fig. 3 (sample to be detected with number 3), fig. 4 (sample to be detected with number 4) and fig. 5 (sample to be detected with number 5), and the red boxes in fig. 1 to 5 are all target regions (i.e., chromosome regions shown in column 3 in table 1). The results show that the samples to be tested with the numbers of 1 to 12 are deleted or repeated in the corresponding target areas, and the fetuses are sequentially identified as Williams-beer syndrome, Wolf-Hirschhorn syndrome, Smith-Magenis syndrome, Miller-Dieker syndrome, peroneal muscular atrophy, 1p36 deletion syndrome, Cat's syndrome, Digeorge syndrome, Duchenne muscular dystrophy, 15q13.3 microdeletion syndrome, Angel's syndrome and Langer-Giedion syndrome.

Thus, the kit prepared in example 1 can be used for non-invasive prenatal detection of whether the fetus has 12 chromosome microdeletion micro-repeat syndromes shown in column 1 of table 1.

<110> Beijing Makino Gene science and technology Co., Ltd

<120> a kit for noninvasive prenatal detection of 12 chromosome microdeletion and microduplication syndrome and special probe set thereof

<160> 608

<170> PatentIn version 3.5

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gactacatgg gacatctcac taaaatttaa ttctgatttg taggccaaag aatgcgaagc 60

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cccgtctgca ccagcagcag cctctcttcc tggtacgtcg taggttcc 108

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gactacatgg gacatcaaag cggccctgga gggctggtct ttattttgat gaggctgaga 60

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gactacatgg gacatgacct ggagcagcgg cacaaggccc aggtgctggt cgaggacatc 60

agtgacatcc tggaggagca cgctgagaag cactacgtcg taggttcc 108

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gactacatgg gacatacagc ccaggggtaa ttccagaagc accagaagag gctgcacgca 60

ccatcctcac agttagttcc actgacaccc gggtacgtcg taggttcc 108

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gactacatgg gacatttccc acctttccat catagctccc aactgccagg aactgactgc 60

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gactacatgg gacatgaaga aactctactg ccagatcgcc aagacatgcc ccatccagat 60

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gactacatgg gacatagcat ggcgtctttc gacaccaagg agactcttca gcagaccctg 60

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gactacatgg gacattcttt cagtgctgcc agctgccctt gtaaagcttt tatttcttct 60

ttcttttgtt tttctgcttc ttctgtagcc gcttacgtcg taggttcc 108

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gactacatgg gacatcgagt aaatgatgac aaagaagtct ttgatctccg ggcttggacc 60

actggccacc ttcagaaata tgtcccgtgg ttctacgtcg taggttcc 108

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gactacatgg gacatcccag gccaggcccg tcttgttggt gggattctga tttgttgttg 60

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gactacatgg gacatgtggt gggcggcagc agcacagacc tggatgtcat tgtgcgggtt 60

ccagtccgag tcgtagatga tgacagtgtc cgctacgtcg taggttcc 108

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<212> DNA

<213> Artificial sequence

<400> 37

gactacatgg gacatccctt gccaggtttc aggcacccgt ggatgagcag tcagagagtc 60

tacagaacac gcacgacgac agcaggaaca acgtacgtcg taggttcc 108

<210> 38

<211> 108

<212> DNA

<213> Artificial sequence

<400> 38

gactacatgg gacattgatt tttcattctg taccacgtgg ttgaaattgg agccagctgt 60

aaacatggcc gccttcctca gacattctcc gaatacgtcg taggttcc 108

<210> 39

<211> 108

<212> DNA

<213> Artificial sequence

<400> 39

gactacatgg gacattgtat aaacacctat cagtttatgt ccagtgaact caactggact 60

ctctttttct tcatcagcaa attccaaagc atctacgtcg taggttcc 108

<210> 40

<211> 108

<212> DNA

<213> Artificial sequence

<400> 40

gactacatgg gacatatgcc agaaggtggg ttctgggagc cgccccacca cgctccacgc 60

gcgagtctct gggtcatagg cctctaccac gtctacgtcg taggttcc 108

<210> 41

<211> 108

<212> DNA

<213> Artificial sequence

<400> 41

gactacatgg gacatcctgg ttgagtgaag gtgacaggca ttccttgacc cgaaaggcct 60

aagttcaact tacttgactt tgagagaaac acctacgtcg taggttcc 108

<210> 42

<211> 108

<212> DNA

<213> Artificial sequence

<400> 42

gactacatgg gacatgcaaa tttttcttca gttaaaaaca gaaatagaag cgacggtttt 60

gacccatttt acacctattt atttcaggct ctctacgtcg taggttcc 108

<210> 43

<211> 108

<212> DNA

<213> Artificial sequence

<400> 43

gactacatgg gacatgagct tcaccttcca ggtgtccacc aaggacgtgc cgctggcgct 60

gatggcctgt gccctgcgga agaaggccac agttacgtcg taggttcc 108

<210> 44

<211> 108

<212> DNA

<213> Artificial sequence

<400> 44

gactacatgg gacattgagt aggtgtctcg gtcgcagcct ttccctatgt ggctggtttc 60

tagctccact gtggcctgta ctgaggcgac tggtacgtcg taggttcc 108

<210> 45

<211> 108

<212> DNA

<213> Artificial sequence

<400> 45

gactacatgg gacatcattt ttaccccacc ggcctgttca gcggcttgac tggctcttcc 60

atcttctgcc taggttgctt ctttgcggtt gtgtacgtcg taggttcc 108

<210> 46

<211> 108

<212> DNA

<213> Artificial sequence

<400> 46

gactacatgg gacatcagtg ccgggcccct cataggagag gaagcccggg aggtggccag 60

gcggcaggaa ggcgcacccc cccagcaatc tgctacgtcg taggttcc 108

<210> 47

<211> 108

<212> DNA

<213> Artificial sequence

<400> 47

gactacatgg gacatacctg tttgctcagc tgccttcttc ttgacagctt ctttagagtc 60

tttttgtact tgtacctggg ccagcttcca gcgtacgtcg taggttcc 108

<210> 48

<211> 108

<212> DNA

<213> Artificial sequence

<400> 48

gactacatgg gacattgcga gtgggggttc caggagagcg caggtctgca gggcccagtg 60

gcctacctcg gagctggggc ttttcagcca cagtacgtcg taggttcc 108

<210> 49

<211> 108

<212> DNA

<213> Artificial sequence

<400> 49

gactacatgg gacatctaga ggaggagcag gaggaggaag aagcaacggg tgaaaccttt 60

ttaggggccc agaagccagg gccccaacct gggtacgtcg taggttcc 108

<210> 50

<211> 108

<212> DNA

<213> Artificial sequence

<400> 50

gactacatgg gacatgtttt tgtttatttg cttttgtttt cacccaaaca ggaggaaatg 60

tgtcggattg aagaggaaca gagctttgag gaatacgtcg taggttcc 108

<210> 51

<211> 108

<212> DNA

<213> Artificial sequence

<400> 51

gactacatgg gacataagcc cttggcagct gccctgtctc tgtctccgca gggacccgag 60

accgtcatgg cagagcagtg gtgcaggtcc gcatacgtcg taggttcc 108

<210> 52

<211> 108

<212> DNA

<213> Artificial sequence

<400> 52

gactacatgg gacattgtct tagaactcag aatccaagaa atggcatcca tgggtatagg 60

caaccagcca ttcatggatg tcaagcccag agatacgtcg taggttcc 108

<210> 53

<211> 108

<212> DNA

<213> Artificial sequence

<400> 53

gactacatgg gacataagat gtgaatgaag aagagtggcc aggccaaggg ttgggggtgc 60

ctaatgtgtc ttttcttctc tgacagtcgt gcatacgtcg taggttcc 108

<210> 54

<211> 108

<212> DNA

<213> Artificial sequence

<400> 54

gactacatgg gacattgcga atcggaactg catggaaaac ccaattactc agcaggtact 60

tagaacattt cttatctgat cttttccaca tggtacgtcg taggttcc 108

<210> 55

<211> 108

<212> DNA

<213> Artificial sequence

<400> 55

gactacatgg gacattgacc gaagcctgtc tgtcctcaga tataaagatg aagcgcagct 60

gtataaagaa gagcacctga ggaatcggca gcatacgtcg taggttcc 108

<210> 56

<211> 108

<212> DNA

<213> Artificial sequence

<400> 56

gactacatgg gacatcagcg actccccgaa gacgatgatg aacaggacct cgttgacatg 60

gacctcctca aacacggcca ggacggccac cggtacgtcg taggttcc 108

<210> 57

<211> 108

<212> DNA

<213> Artificial sequence

<400> 57

gactacatgg gacatcccgc agttggtaca gtaccagtgt ggggactctg ggaagcaggg 60

ccgtgagacg aggaggagca gctgcagagg gtgtacgtcg taggttcc 108

<210> 58

<211> 108

<212> DNA

<213> Artificial sequence

<400> 58

gactacatgg gacatcctgg tcagcaccaa tgaacacctg ctgcaggagc tgagccaggt 60

gcgggcgcag cacagagccg aggtggagca gattacgtcg taggttcc 108

<210> 59

<211> 108

<212> DNA

<213> Artificial sequence

<400> 59

gactacatgg gacatgcaga ggccgcaggc tctggagact gaagaaactc ataggggtcg 60

tgggtgacgt ctggctgctc cccgacactc aggtacgtcg taggttcc 108

<210> 60

<211> 108

<212> DNA

<213> Artificial sequence

<400> 60

gactacatgg gacatctcgg ctttttggat ttcttggcag tttctacttg tactggtaca 60

acttcaggga caggttcctc aacagctgta tcttacgtcg taggttcc 108

<210> 61

<211> 108

<212> DNA

<213> Artificial sequence

<400> 61

gactacatgg gacatacatc tgatcctggg cttccccatc tggtgctttt cccatggaga 60

acacacaacc agtaagtgag gttgccccac acatacgtcg taggttcc 108

<210> 62

<211> 108

<212> DNA

<213> Artificial sequence

<400> 62

gactacatgg gacatggctt tgcacgaaaa ctgcaggtgg aaacgtactt atctcgtaag 60

accacgcctt caatgcaggc cccaaacagc acatacgtcg taggttcc 108

<210> 63

<211> 108

<212> DNA

<213> Artificial sequence

<400> 63

gactacatgg gacatgtatg tggacgagtg cgccaggagc tcccctgtgg actacttctg 60

gtaccgagag acgctcaaca tctccacgtc cattacgtcg taggttcc 108

<210> 64

<211> 108

<212> DNA

<213> Artificial sequence

<400> 64

gactacatgg gacatcgtac agaaagctta gcgagttgaa gcccagctgg tagcagcagt 60

gacagctgtc tctggccttg gcaggttcaa taatacgtcg taggttcc 108

<210> 65

<211> 108

<212> DNA

<213> Artificial sequence

<400> 65

gactacatgg gacattccct tacctggtcc tgaccatctt tctcatcaga gggctgaccc 60

tgccaggggc aacaaaagga ctcatctact tgttacgtcg taggttcc 108

<210> 66

<211> 108

<212> DNA

<213> Artificial sequence

<400> 66

gactacatgg gacattttca ggatggagta gcagagggag gccgtgtcag agatgacgcg 60

caggaaaaat gtggggttct tccaaacttg ctgtacgtcg taggttcc 108

<210> 67

<211> 108

<212> DNA

<213> Artificial sequence

<400> 67

gactacatgg gacataacac tgaatgcatc aaaagcaaat caacccccac ccaagccccc 60

ctggggaaga ggaggcctca cccgtcccgc cgatacgtcg taggttcc 108

<210> 68

<211> 108

<212> DNA

<213> Artificial sequence

<400> 68

gactacatgg gacatccgtg tggggcgccc gcgtggcctt ctcctcgtag gactccccaa 60

actcgttcac tctgcgttta tccacaggat aaatacgtcg taggttcc 108

<210> 69

<211> 108

<212> DNA

<213> Artificial sequence

<400> 69

gactacatgg gacattcagg tcactgcccg gaactcacct caatggcggc tccaacaccc 60

gccgggacca gcaccagcag gctcgtctgc tcgtacgtcg taggttcc 108

<210> 70

<211> 108

<212> DNA

<213> Artificial sequence

<400> 70

gactacatgg gacattggag aaagacatgt ccgttctgac ggagaggcac agcctgttat 60

cagtaaccca tgaagaccct cacctgtgta tcatacgtcg taggttcc 108

<210> 71

<211> 108

<212> DNA

<213> Artificial sequence

<400> 71

gactacatgg gacatgctca cggtccttct cgggtgcaat ggcgtaggcc agtttctgaa 60

agagaaagag agtgcagggg tcagtgcaga ccatacgtcg taggttcc 108

<210> 72

<211> 108

<212> DNA

<213> Artificial sequence

<400> 72

gactacatgg gacatgactc ccacttactc aaagtactcg gcagcaggtg tggtcccaaa 60

agtgtcgttg aggcccgagc tgtctccact ggatacgtcg taggttcc 108

<210> 73

<211> 108

<212> DNA

<213> Artificial sequence

<400> 73

gactacatgg gacatgagaa atgaacaatg gtgctgacgt gactgcaccg ccctgtcccc 60

cagtcaaacc catgtgaact ctgcactcac ctttacgtcg taggttcc 108

<210> 74

<211> 108

<212> DNA

<213> Artificial sequence

<400> 74

gactacatgg gacattcgat ttccacttgg ttgtgaaact gacacagcgt gagccacagg 60

atttccagcc ttaaaaacag attgcacaat gtttacgtcg taggttcc 108

<210> 75

<211> 108

<212> DNA

<213> Artificial sequence

<400> 75

gactacatgg gacataatga aaactttgcc attgatttga tagcagagca gcccgtgagc 60

gaggtggaga ctcgggtgat agcgtgcgat ggctacgtcg taggttcc 108

<210> 76

<211> 108

<212> DNA

<213> Artificial sequence

<400> 76

gactacatgg gacatgcgcc ggcgcctcaa gaaggagaac aaggtgacat ggggagcgcg 60

cagcaaggac caggaagatg gagcgctctt cggtacgtcg taggttcc 108

<210> 77

<211> 108

<212> DNA

<213> Artificial sequence

<400> 77

gactacatgg gacattcttc tgaggtccca tagaaatgaa gaactgaacg tggagacctt 60

ggtggtggtc gacaaaaaga tgatgcaaaa ccatacgtcg taggttcc 108

<210> 78

<211> 108

<212> DNA

<213> Artificial sequence

<400> 78

gactacatgg gacatgcagt tactagtagc tcatctcctc tttgtttcag caccgctcaa 60

gctatctgcc ttgctgatca gccaaagcct gtgtacgtcg taggttcc 108

<210> 79

<211> 108

<212> DNA

<213> Artificial sequence

<400> 79

gactacatgg gacattgaaa tgaacgtctc tacctcctac atttctgtgc gcaatgccct 60

cagaaggtac tacctgaatg ggcactggac cgttacgtcg taggttcc 108

<210> 80

<211> 108

<212> DNA

<213> Artificial sequence

<400> 80

gactacatgg gacatgaatt taagaagaca caggtactag ataaaagcag catacacaca 60

ccttaaatac gtggctccag ggtctctgta gtgtacgtcg taggttcc 108

<210> 81

<211> 108

<212> DNA

<213> Artificial sequence

<400> 81

gactacatgg gacatacacc acaaaatgct aatggacttt ggaaattgaa atctacaact 60

cccggtggtg ctttgcctga gtgttttggc acctacgtcg taggttcc 108

<210> 82

<211> 108

<212> DNA

<213> Artificial sequence

<400> 82

gactacatgg gacatcttga gtcccagcaa tccagcagaa atttccaaga tcctggaagc 60

ttggcggaga gaggcctcca aaagcgttcc gtctacgtcg taggttcc 108

<210> 83

<211> 108

<212> DNA

<213> Artificial sequence

<400> 83

gactacatgg gacataaccc cttccttctt cttttctccc aatacctcca gccccatcat 60

cctgagataa tgaagccgtt cattcttggg cactacgtcg taggttcc 108

<210> 84

<211> 108

<212> DNA

<213> Artificial sequence

<400> 84

gactacatgg gacataccat tttgaacaca agtccaatgc atacctggaa aggggacatt 60

catgtcggca tgtagcattt caattaactg tgttacgtcg taggttcc 108

<210> 85

<211> 108

<212> DNA

<213> Artificial sequence

<400> 85

gactacatgg gacattcatc atcttcgtgg tgtacaccat gctgcccttc aacatgcgag 60

acgccatcat tgccagcgtc ctcacctcct ccttacgtcg taggttcc 108

<210> 86

<211> 108

<212> DNA

<213> Artificial sequence

<400> 86

gactacatgg gacatgagta actggacggt tgtcattgct tcacagcttc tttccaagcc 60

aaaattcagt ggagttgaaa agattaagac cattacgtcg taggttcc 108

<210> 87

<211> 108

<212> DNA

<213> Artificial sequence

<400> 87

gactacatgg gacatcacgg ggatgcttca ggatggagca atgtgcccaa agccgggttc 60

cttgaggctg gggatgtcgt tcttcctgta gaatacgtcg taggttcc 108

<210> 88

<211> 108

<212> DNA

<213> Artificial sequence

<400> 88

gactacatgg gacataacac tggtccaccg agtggatgaa ggtcatttcc atttttcgaa 60

tgaaactttt tacaatgggc atgatggaat ttgtacgtcg taggttcc 108

<210> 89

<211> 108

<212> DNA

<213> Artificial sequence

<400> 89

gactacatgg gacatctcga ggacagacct tgtgaagtca gagctgctac acattgaatc 60

tcaagtcgag cttctgagat tcgatgattc aggtacgtcg taggttcc 108

<210> 90

<211> 108

<212> DNA

<213> Artificial sequence

<400> 90

gactacatgg gacattgctc ctgttgggga ggaggaagcc ccagcaaagt atgtgcaaga 60

caacatccag cttcatggcc agcaggtggc gagtacgtcg taggttcc 108

<210> 91

<211> 108

<212> DNA

<213> Artificial sequence

<400> 91

gactacatgg gacattgcgt catgctcagg ctctcctcca ggcttgtgca tttcttcatt 60

accacatccc agccacagta agggtcctgg gcctacgtcg taggttcc 108

<210> 92

<211> 108

<212> DNA

<213> Artificial sequence

<400> 92

gactacatgg gacatgacgg aaggcttacc attgagccat ttggagttgt actgcgccgt 60

gcggagagga ggtaaaatgc ctaggcttcg gtatacgtcg taggttcc 108

<210> 93

<211> 108

<212> DNA

<213> Artificial sequence

<400> 93

gactacatgg gacatcagct gtaagtctct acaagatcag ggaagactac aatatttcag 60

aaaaacactc ccagtcaagt ttatgcttga atgtacgtcg taggttcc 108

<210> 94

<211> 108

<212> DNA

<213> Artificial sequence

<400> 94

gactacatgg gacatacaat gcctgaaaaa caagcacaga ggcatgatgt ctgatcccca 60

cactcatgcc acatgatccc cacacccatg ccgtacgtcg taggttcc 108

<210> 95

<211> 108

<212> DNA

<213> Artificial sequence

<400> 95

gactacatgg gacatgtgtg gttgagagag cagatagtcc atgggggagc accaatttgg 60

ttggagcatg ctgccccacc gttcaatgct gcgtacgtcg taggttcc 108

<210> 96

<211> 108

<212> DNA

<213> Artificial sequence

<400> 96

gactacatgg gacatctcag aaagatcctc tttaatgatg tgggctcctg tttcctaata 60

gatcatgaag actttgatag ttgcggtgct gtttacgtcg taggttcc 108

<210> 97

<211> 108

<212> DNA

<213> Artificial sequence

<400> 97

gactacatgg gacatgtagt tgccggtgga cttgagaccc aggtgcatgg tgtactcgct 60

ggcgggaggg cgatggtgga cctggtcctc gaatacgtcg taggttcc 108

<210> 98

<211> 108

<212> DNA

<213> Artificial sequence

<400> 98

gactacatgg gacatgccga ccaacttctc gatgccaccg gcatcccgta aggccttggc 60

gttctccatg ttcttggtaa tcacttcgtg cagtacgtcg taggttcc 108

<210> 99

<211> 108

<212> DNA

<213> Artificial sequence

<400> 99

gactacatgg gacattatgc cagcacaacc aaccttgcta tcgatctcac tgctccccag 60

cgcagactgg atcacgtaca gcaaggcatc cgttacgtcg taggttcc 108

<210> 100

<211> 108

<212> DNA

<213> Artificial sequence

<400> 100

gactacatgg gacataaacc tctttagaca tcatagggaa agaaaaaggt ttttacccac 60

ctgggatctt tctgaatgct atcaatggac gggtacgtcg taggttcc 108

<210> 101

<211> 108

<212> DNA

<213> Artificial sequence

<400> 101

gactacatgg gacatagggt ctcagctctt gggatcacat tgacacaccc ccacatttta 60

cactctgtgg cccattgctt ctgagctgta ttgtacgtcg taggttcc 108

<210> 102

<211> 108

<212> DNA

<213> Artificial sequence

<400> 102

gactacatgg gacattttgg caaggacatt tcacatggtt tgtgggtgaa tagtttcaca 60

ccagagtggg atcctctatt gcatgtactc gactacgtcg taggttcc 108

<210> 103

<211> 108

<212> DNA

<213> Artificial sequence

<400> 103

gactacatgg gacatccgag gggctggcat aaagagattg ccatggggca atggctactg 60

gcacagatag ggcccctcca agaagcacat ggttacgtcg taggttcc 108

<210> 104

<211> 108

<212> DNA

<213> Artificial sequence

<400> 104

gactacatgg gacatccgca aaagactatt agcttttaat tgaaaccctc cctccacccc 60

cacccccagc cccttccagg aacaggaaca ggttacgtcg taggttcc 108

<210> 105

<211> 108

<212> DNA

<213> Artificial sequence

<400> 105

gactacatgg gacatgtggg gatgttcagt ggtaaagtct cgtgtttctc caaagccctg 60

ggaccgccat tccccagtgg gaaactgact tggtacgtcg taggttcc 108

<210> 106

<211> 108

<212> DNA

<213> Artificial sequence

<400> 106

gactacatgg gacataccat gactaggagc aggcaggtgg cttagagagg tcaggaaggc 60

tatgcttgca aagtggaatt gaagccagag gagtacgtcg taggttcc 108

<210> 107

<211> 108

<212> DNA

<213> Artificial sequence

<400> 107

gactacatgg gacattcctt tcagtctatg cccaggctgg cccacagggg ccctggtgga 60

gccctttccc tccccctcac ttcctcttct tgttacgtcg taggttcc 108

<210> 108

<211> 108

<212> DNA

<213> Artificial sequence

<400> 108

gactacatgg gacattcgtg tgtgtgcaac agctgttggg aacattagga ctgccatctg 60

tgtccaatgg aatgatttcc taccacttgg ccttacgtcg taggttcc 108

<210> 109

<211> 108

<212> DNA

<213> Artificial sequence

<400> 109

gactacatgg gacatatatg catggcgggc acctgaaaaa tgggcaaaac catagtccct 60

ccgtcctaca acacttgtac aactcccact gaatacgtcg taggttcc 108

<210> 110

<211> 108

<212> DNA

<213> Artificial sequence

<400> 110

gactacatgg gacatctatg acagccagct ctggcaaatt tcctggccag actttcagct 60

aatgaagaga catactgggc ttttaggttc ccttacgtcg taggttcc 108

<210> 111

<211> 108

<212> DNA

<213> Artificial sequence

<400> 111

gactacatgg gacatcattt tcccccttgt gactgtgttg tgttgtacgc tatccaaaaa 60

tccagttccc caccaatgat tgtctcaaca ctgtacgtcg taggttcc 108

<210> 112

<211> 108

<212> DNA

<213> Artificial sequence

<400> 112

gactacatgg gacatgatgg tgccaatcat ggctggaaag caatgcacag aaatcttgga 60

gacacgacag aaaagcagtg tggaagcagg tgttacgtcg taggttcc 108

<210> 113

<211> 108

<212> DNA

<213> Artificial sequence

<400> 113

gactacatgg gacatccgtg tgtcagagtg gcggggtctg caggtctctc attcttcctc 60

tgcctaacgt gcattgctag gcaacccaca tagtacgtcg taggttcc 108

<210> 114

<211> 108

<212> DNA

<213> Artificial sequence

<400> 114

gactacatgg gacatctggc agtgtgtctc agagacttgc tcattacgca ccatgaatag 60

caacgtcccc tggttattgc aatcagtgtc cattacgtcg taggttcc 108

<210> 115

<211> 108

<212> DNA

<213> Artificial sequence

<400> 115

gactacatgg gacatgggtc tgtgcaggcc cacccctaga tggcttgcct ggtgggcccc 60

taaatgttat atctgcatca cagaaggact ctgtacgtcg taggttcc 108

<210> 116

<211> 108

<212> DNA

<213> Artificial sequence

<400> 116

gactacatgg gacatccata aaacaacgca taaaaatgtg tcttcccatg ccatttatac 60

ggaaagatgg tgcagttcaa tccctcaagc caatacgtcg taggttcc 108

<210> 117

<211> 108

<212> DNA

<213> Artificial sequence

<400> 117

gactacatgg gacatggggc ccttgagtct cgggatctct ttcagtaggc tcaggctggt 60

gctaggcttt tcactgggac cttagcagtg tcctacgtcg taggttcc 108

<210> 118

<211> 108

<212> DNA

<213> Artificial sequence

<400> 118

gactacatgg gacatctgcg tgtgatccct ctttgctttg agtcacccgc tctcagcttc 60

actgctcagt gcagcagagg ctcctatgag cagtacgtcg taggttcc 108

<210> 119

<211> 108

<212> DNA

<213> Artificial sequence

<400> 119

gactacatgg gacatccgct ctctggattt tgcatatatc tggtggttta tttgtttcat 60

cgattccaga gtccagtgga aacagagtag gtttacgtcg taggttcc 108

<210> 120

<211> 108

<212> DNA

<213> Artificial sequence

<400> 120

gactacatgg gacatgtcaa tttgacttac acattcctga gtttgtcatc tcagcagctc 60

ccagggaaag cacctggagt taaatttcca tgatacgtcg taggttcc 108

<210> 121

<211> 108

<212> DNA

<213> Artificial sequence

<400> 121

gactacatgg gacatagggg aattctaagg gtgggtgtta ttttaacctt tgaagggcaa 60

aatagaaatt ggcacatcac acggatggtc aaatacgtcg taggttcc 108

<210> 122

<211> 108

<212> DNA

<213> Artificial sequence

<400> 122

gactacatgg gacatgcgtt tccgcaagat cacatagatg acaccgctga gaagggccag 60

ggggaaggcc acccaggcca ggatgtaggc gaatacgtcg taggttcc 108

<210> 123

<211> 108

<212> DNA

<213> Artificial sequence

<400> 123

gactacatgg gacatagtaa tccgagttga gatgccactc cgggtgcctc accgtgtaga 60

tggccgcagc actcatcacg cacagacctg gggtacgtcg taggttcc 108

<210> 124

<211> 108

<212> DNA

<213> Artificial sequence

<400> 124

gactacatgg gacatgggcg taatgcctac ggaggatgct acccaaatgc aggattcagt 60

agaaggctgg ctgtggggtg agggagagag gagtacgtcg taggttcc 108

<210> 125

<211> 108

<212> DNA

<213> Artificial sequence

<400> 125

gactacatgg gacatgcccc atgtttggaa atgacaatat gtgacacaag agtgtcaaaa 60

tgaagacacc agaggatgac aacagtcaat caatacgtcg taggttcc 108

<210> 126

<211> 108

<212> DNA

<213> Artificial sequence

<400> 126

gactacatgg gacatttggt gagggtgaag agttggcaga agaacaggaa cagagacaga 60

atgctgaaga tgatcgacag gatcatggtg gcctacgtcg taggttcc 108

<210> 127

<211> 108

<212> DNA

<213> Artificial sequence

<400> 127

gactacatgg gacattcatg aggagtatgg ctggtggaga caatatctta gatttccttc 60

tccaatcctg agatactgtc ttcaccctta acctacgtcg taggttcc 108

<210> 128

<211> 108

<212> DNA

<213> Artificial sequence

<400> 128

gactacatgg gacatacaga tcgccatttt ccttatttct cagtatgaat gtcattccag 60

aaatagctga gtctctgaaa ggcctccatc ctatacgtcg taggttcc 108

<210> 129

<211> 108

<212> DNA

<213> Artificial sequence

<400> 129

gactacatgg gacatgcaca ctcatgtggc ccccagcact ggcagggacc tgaccacagt 60

cagaaggctt catcccagac agccttctct cggtacgtcg taggttcc 108

<210> 130

<211> 108

<212> DNA

<213> Artificial sequence

<400> 130

gactacatgg gacatagcct ctcttacctc tgcccacacg aggcagataa ggagggggtg 60

ccagaaagta tacaaacaca tttagaaaaa cgatacgtcg taggttcc 108

<210> 131

<211> 108

<212> DNA

<213> Artificial sequence

<400> 131

gactacatgg gacatccgtt tggtgatgat gagaaacagt ggtggacatt tcctgaggaa 60

gaggtgctac agttctgcca gagatcagtt gcgtacgtcg taggttcc 108

<210> 132

<211> 108

<212> DNA

<213> Artificial sequence

<400> 132

gactacatgg gacatacgat cgtggagacg aacagcagca ccagcaccgc gacgtggagg 60

acgatgatac tcagcaacag gaggagcatt ctgtacgtcg taggttcc 108

<210> 133

<211> 108

<212> DNA

<213> Artificial sequence

<400> 133

gactacatgg gacattatag accataaagg ctctcctcgt tgtattactc caagacccat 60

tggagggaaa caggacacac aggggaccct gtgtacgtcg taggttcc 108

<210> 134

<211> 108

<212> DNA

<213> Artificial sequence

<400> 134

gactacatgg gacatgtaca gggaattggc tttgacagcc aggtcatact cgagtgtggc 60

tttgatgtgg accagcgact gcagggtgtc ctctacgtcg taggttcc 108

<210> 135

<211> 108

<212> DNA

<213> Artificial sequence

<400> 135

gactacatgg gacataaggc ttctggagtt cacgcactgc ccttggctcc tccagttgct 60

atcaagttga tttacttctg catggagcat ctttacgtcg taggttcc 108

<210> 136

<211> 108

<212> DNA

<213> Artificial sequence

<400> 136

gactacatgg gacattctgg ctcaactaat atttgttgcc taaagcaggc accatgagaa 60

ggaaaagtaa atcgctgcag cctgagaaaa tgttacgtcg taggttcc 108

<210> 137

<211> 108

<212> DNA

<213> Artificial sequence

<400> 137

gactacatgg gacattgcta cttctgtgct gaggctcttg ccttaggtga ttatgctgaa 60

attctaggac tagaaggcct ttctcctttg gtctacgtcg taggttcc 108

<210> 138

<211> 108

<212> DNA

<213> Artificial sequence

<400> 138

gactacatgg gacattagtg agcagaggca tttcctttca ttagagatct gcattctcag 60

tatttattcc tgagttcagc caagatgacc cattacgtcg taggttcc 108

<210> 139

<211> 108

<212> DNA

<213> Artificial sequence

<400> 139

gactacatgg gacatttcaa tggaggaggg tggaatgggg ttggagggcc catcgcatag 60

cctgagggtt agaggtgacc tgagcccatt cagtacgtcg taggttcc 108

<210> 140

<211> 108

<212> DNA

<213> Artificial sequence

<400> 140

gactacatgg gacatcacaa gtttacatga cttgccccac accctgcctt agatcagggg 60

caagcatagg cttgaagggc tcacatacaa acctacgtcg taggttcc 108

<210> 141

<211> 108

<212> DNA

<213> Artificial sequence

<400> 141

gactacatgg gacatgtggg atagggcatt tgcatggatc tggcagagtc caaattcaaa 60

cgcctggagc aaaatgagca gcaaccccgt tcctacgtcg taggttcc 108

<210> 142

<211> 108

<212> DNA

<213> Artificial sequence

<400> 142

gactacatgg gacatagtat ttccacaacc accacctgcc aataccaaaa ttgacttaga 60

tcaaagaaga agcagtggct ttaaagtaca gagtacgtcg taggttcc 108

<210> 143

<211> 108

<212> DNA

<213> Artificial sequence

<400> 143

gactacatgg gacattcata atatctaatc aatcagaaaa aggaagtcct gctccgttga 60

aacaataggg acattgtgaa cctggagaac acttacgtcg taggttcc 108

<210> 144

<211> 108

<212> DNA

<213> Artificial sequence

<400> 144

gactacatgg gacatatcta cctcccaaga ggagtaaggg taaacatgga aatcgttttc 60

ctctgcccct cacaactcac atcttaacag aactacgtcg taggttcc 108

<210> 145

<211> 108

<212> DNA

<213> Artificial sequence

<400> 145

gactacatgg gacatcagca tgacagtctg tctcatcctg gccttcactc ctgtatcccc 60

gtgacagcca cggtgttcac ccattcctga acctacgtcg taggttcc 108

<210> 146

<211> 108

<212> DNA

<213> Artificial sequence

<400> 146

gactacatgg gacatcctcc ttaacaccgt cttctgagca tcctcccgcc tgcccgtctg 60

tcatcagaga tgtgggtggc ctgaggaaat gggtacgtcg taggttcc 108

<210> 147

<211> 108

<212> DNA

<213> Artificial sequence

<400> 147

gactacatgg gacatgggcg ctgggccagc ttccacccag atggggagaa gggaagggct 60

gcctcatctg cagaaacgag tctcttctgt tcttacgtcg taggttcc 108

<210> 148

<211> 108

<212> DNA

<213> Artificial sequence

<400> 148

gactacatgg gacatttctc cctcctctgt gggcagcaaa ttccccgtga gtcaacagat 60

cctgtatctg ggctcaccat gggcttctac tggtacgtcg taggttcc 108

<210> 149

<211> 108

<212> DNA

<213> Artificial sequence

<400> 149

gactacatgg gacatggggc tgtccactct caccacgaat aaacttgctc aatgacggat 60

gagaagccgc tctcaactcg catttcctgg aactacgtcg taggttcc 108

<210> 150

<211> 108

<212> DNA

<213> Artificial sequence

<400> 150

gactacatgg gacatttgga ggcaggtggg cctcccttct tggggtgagc agcccagcaa 60

gcaggccggc gaggagcttc atgcagcatc ctttacgtcg taggttcc 108

<210> 151

<211> 108

<212> DNA

<213> Artificial sequence

<400> 151

gactacatgg gacatgccca gacattcccc aagaacgcca gctcaagggg gcttacttct 60

tttacacaga aatgaagact tctcgtagca gcttacgtcg taggttcc 108

<210> 152

<211> 108

<212> DNA

<213> Artificial sequence

<400> 152

gactacatgg gacatgctcc cagacaaagg actgtcccag aggctcctga ccgggccaac 60

accatccaaa ccctcatttg gaaaggcaaa cactacgtcg taggttcc 108

<210> 153

<211> 108

<212> DNA

<213> Artificial sequence

<400> 153

gactacatgg gacatttatt tcaaaaccta agttgaacac aagatttaac acacgaaaaa 60

ctctatccaa aaaacggggt tacagttaag gtttacgtcg taggttcc 108

<210> 154

<211> 108

<212> DNA

<213> Artificial sequence

<400> 154

gactacatgg gacatgctgg gtgtacagct gccctgcagg ctctgtgaag cgtctatgag 60

cccagcccag gcctggctct aaaagaagtc cgatacgtcg taggttcc 108

<210> 155

<211> 108

<212> DNA

<213> Artificial sequence

<400> 155

gactacatgg gacatagcag ctccccttcg gggacttctt gacgccgggc aggagcctcg 60

cggaagccga cgcgcgcttc tggtgtcgcc atttacgtcg taggttcc 108

<210> 156

<211> 108

<212> DNA

<213> Artificial sequence

<400> 156

gactacatgg gacatcacct ccttccacgt ccgggtgctg ctggccttgc ggctgttgtc 60

cactgctgcc tgatactcac cgaggtgaac caatacgtcg taggttcc 108

<210> 157

<211> 108

<212> DNA

<213> Artificial sequence

<400> 157

gactacatgg gacatagtca ccttcaaggt gtaccaggtg gctggactat acctggagaa 60

atgtctgatg tctgggaacc tggatgtaga acctacgtcg taggttcc 108

<210> 158

<211> 108

<212> DNA

<213> Artificial sequence

<400> 158

gactacatgg gacattagga gttgcttacg cccctgctga agaaccagat ggcaaagttc 60

taaggattca agtttattga gctgaccctg gtctacgtcg taggttcc 108

<210> 159

<211> 108

<212> DNA

<213> Artificial sequence

<400> 159

gactacatgg gacataaact atgggttcca aattactcta taaaattcct caacaacctc 60

tcaaaacagt gctagaaata gtttatacaa tgatacgtcg taggttcc 108

<210> 160

<211> 108

<212> DNA

<213> Artificial sequence

<400> 160

gactacatgg gacatcaggg ctgccaggag cctcaccttc gtttacgagg taccgtgcgt 60

agacgaggag ccaatggcgg tactcgtggc tggtacgtcg taggttcc 108

<210> 161

<211> 108

<212> DNA

<213> Artificial sequence

<400> 161

gactacatgg gacataagac aggaagatct cgccagataa gaccatgaaa ggggaatgaa 60

ccagtaccca gtgtccagct gtgctatgca gagtacgtcg taggttcc 108

<210> 162

<211> 108

<212> DNA

<213> Artificial sequence

<400> 162

gactacatgg gacatcccgc ttccacacca taatcagttt gtcatctccc ccagaagcta 60

aatacatccc actgtttgac caccgcacac agttacgtcg taggttcc 108

<210> 163

<211> 108

<212> DNA

<213> Artificial sequence

<400> 163

gactacatgg gacatctggg aacttggcag acgcagctta gagagactca ccagcgagcg 60

tcattgttgt ctttctggga actcattccc atgtacgtcg taggttcc 108

<210> 164

<211> 108

<212> DNA

<213> Artificial sequence

<400> 164

gactacatgg gacatacaga cctacctttt aatatctcca ggcttgattg gggaggggct 60

gggctctacc cctttcttct ttccatccag tcttacgtcg taggttcc 108

<210> 165

<211> 108

<212> DNA

<213> Artificial sequence

<400> 165

gactacatgg gacatccttc ctttctttcg tcaaggaggg caagtccggc tgagacagaa 60

tgcgagccac gttcaggacc ggcggacgcg acctacgtcg taggttcc 108

<210> 166

<211> 108

<212> DNA

<213> Artificial sequence

<400> 166

gactacatgg gacatccctg ttacaggagt aacctggaca agctgtacca tggcctggaa 60

ctcgccaaga agcagctgcg agccacccag cagtacgtcg taggttcc 108

<210> 167

<211> 108

<212> DNA

<213> Artificial sequence

<400> 167

gactacatgg gacataaggg gaacaagttt tgcagatgca cccgatttga ccggtagaca 60

aaggcgggtg ccgcgctgtg tctaatgtac acatacgtcg taggttcc 108

<210> 168

<211> 108

<212> DNA

<213> Artificial sequence

<400> 168

gactacatgg gacatccgct gttcccccac aggctccccc tcagctgttg gacttccccc 60

aaacatcctc ctccaggttc cctagctggt ccatacgtcg taggttcc 108

<210> 169

<211> 108

<212> DNA

<213> Artificial sequence

<400> 169

gactacatgg gacatttcat ctctggactg gaacatgccc cataaattac agtcctaagt 60

tccagcagct gtttcccaaa cacaccagga gagtacgtcg taggttcc 108

<210> 170

<211> 108

<212> DNA

<213> Artificial sequence

<400> 170

gactacatgg gacatcctgg ctccagcttg gagcatgaag ggtccagtgg agatggtgga 60

gatgcagagg cagacctgcc tgcctccctg tgatacgtcg taggttcc 108

<210> 171

<211> 108

<212> DNA

<213> Artificial sequence

<400> 171

gactacatgg gacatgcgct tggagatgcg cagcttgact acctcctcag agcatgtggg 60

atggataccc acggtccgca tcacctgcgc atatacgtcg taggttcc 108

<210> 172

<211> 108

<212> DNA

<213> Artificial sequence

<400> 172

gactacatgg gacatgatgc tcacctcttt cccacctttg gcaacgccgc aaaccgtgtg 60

ctcgtcaaca aagctggctt tgatgttaaa gtatacgtcg taggttcc 108

<210> 173

<211> 108

<212> DNA

<213> Artificial sequence

<400> 173

gactacatgg gacatgctga cctgctgaga gaagggatga gaggtgggac ggactgtctg 60

tctggttaaa cgtggtacag gatcaatttt atctacgtcg taggttcc 108

<210> 174

<211> 108

<212> DNA

<213> Artificial sequence

<400> 174

gactacatgg gacattgtgc gcatggcccg cctgctgtca ccaggggcga ggctcatcac 60

catcgagatc aaccccgact gtgccgccat cactacgtcg taggttcc 108

<210> 175

<211> 108

<212> DNA

<213> Artificial sequence

<400> 175

gactacatgg gacatagcgc cggcggcagc ctccagggtg ttgaagttcc ggctctccgt 60

gaggagggag aggtagagac gtaccacctc gggtacgtcg taggttcc 108

<210> 176

<211> 108

<212> DNA

<213> Artificial sequence

<400> 176

gactacatgg gacataactg gggaccaagc caggcacatg tgcatatgca tgtgcgtgca 60

tgtgaatatg agtgtgtgtg tgcacctgag atgtacgtcg taggttcc 108

<210> 177

<211> 108

<212> DNA

<213> Artificial sequence

<400> 177

gactacatgg gacatcctcc tgcctgcctc ttcctccagc tgctctttgt ggtctggttc 60

tggccgcctg ggcctcaggg ccaaccctgc atgtacgtcg taggttcc 108

<210> 178

<211> 108

<212> DNA

<213> Artificial sequence

<400> 178

gactacatgg gacatgactg ctgtgtcact gtcccctgcc tggaatgttt gtagagtaga 60

tgccttcccc gagacgacag ctctgtggac acatacgtcg taggttcc 108

<210> 179

<211> 108

<212> DNA

<213> Artificial sequence

<400> 179

gactacatgg gacatgcttg tccctttggc gggagtgttg gtgacggggt aggcataggg 60

ggtgagagtg ctgataagaa ggatgaggag aattacgtcg taggttcc 108

<210> 180

<211> 108

<212> DNA

<213> Artificial sequence

<400> 180

gactacatgg gacatatccg ctctgcaccc ccagactcca ctctctgccc ccactgtcta 60

ctgcaggtgc ccgtggttga cacgagctgt gtctacgtcg taggttcc 108

<210> 181

<211> 108

<212> DNA

<213> Artificial sequence

<400> 181

gactacatgg gacataaatg agggtgatgc agagtgagga gtggggagca cagtggcagg 60

ctgctcccct ggagaaacac ccagagaaag gggtacgtcg taggttcc 108

<210> 182

<211> 108

<212> DNA

<213> Artificial sequence

<400> 182

gactacatgg gacattgatc acatcaccct ctgtgcaaaa ggcctttctt ccccggctgc 60

cctctgagtc aggcatctgg tcacctcgaa ccctacgtcg taggttcc 108

<210> 183

<211> 108

<212> DNA

<213> Artificial sequence

<400> 183

gactacatgg gacatggagc agggagcggg gcagggtgag cacacaggcc gccaggcctg 60

agatggagtt gtccagctgg ggcccttcct ccctacgtcg taggttcc 108

<210> 184

<211> 108

<212> DNA

<213> Artificial sequence

<400> 184

gactacatgg gacatcagca gtagagaggt cgctgagaaa tggccttctc ctccttggcc 60

aggatcacta tgcttgattc caagtggcat ggctacgtcg taggttcc 108

<210> 185

<211> 108

<212> DNA

<213> Artificial sequence

<400> 185

gactacatgg gacatagccc agccaggggg atcagaggcg cagtttcaga tccagggctt 60

tctcccctgg aaaggagttg ttgtggttga ccctacgtcg taggttcc 108

<210> 186

<211> 108

<212> DNA

<213> Artificial sequence

<400> 186

gactacatgg gacatacagt cgcagaccca gcctttggtg tcacaggcgc aagctctccc 60

tggacaaatg ttgtataccc aaccaccact gaatacgtcg taggttcc 108

<210> 187

<211> 108

<212> DNA

<213> Artificial sequence

<400> 187

gactacatgg gacatccgca gccctcccag agcccagtga cggcgcggac cccacagaac 60

ttcagtgtcc cctcacctgg acctttaaac acatacgtcg taggttcc 108

<210> 188

<211> 108

<212> DNA

<213> Artificial sequence

<400> 188

gactacatgg gacattggca agcaccctgc gctggacctc tggtgaggcc tgtgtcaggc 60

tgtggtgccc gggggcccac ctggcaggca gcttacgtcg taggttcc 108

<210> 189

<211> 108

<212> DNA

<213> Artificial sequence

<400> 189

gactacatgg gacataagaa cacgcaactg tgggactgac tgtctctgaa gtgaccggcc 60

tcccaggata aggtcccaag tgatggtgga gattacgtcg taggttcc 108

<210> 190

<211> 108

<212> DNA

<213> Artificial sequence

<400> 190

gactacatgg gacatggtgg ggttcatgcc gctgtccacc caagtccgct tcactgtcga 60

ccgccccttt cttttcctca tctacgagca tcgtacgtcg taggttcc 108

<210> 191

<211> 108

<212> DNA

<213> Artificial sequence

<400> 191

gactacatgg gacataacgc ctccacaatc actgggtcca ccgtcaatcc agccttcagg 60

cacctgagga gcaagaaaac ccatgtggca ctgtacgtcg taggttcc 108

<210> 192

<211> 108

<212> DNA

<213> Artificial sequence

<400> 192

gactacatgg gacatagagg aatagaagga caaggaatgc tatctacttt gcagctctgc 60

ccatgactct cggtatccca ggatcgaggg ccctacgtcg taggttcc 108

<210> 193

<211> 108

<212> DNA

<213> Artificial sequence

<400> 193

gactacatgg gacatggaaa atatgcaaat gcactggccc agcaacagag ctcctgggtc 60

cagaggaggg acatgataga ctgggtgtgt tagtacgtcg taggttcc 108

<210> 194

<211> 108

<212> DNA

<213> Artificial sequence

<400> 194

gactacatgg gacattgggg actatgtgct gtcggtgtcc gagaactcgc gggtctccca 60

ctacatcatc aactcgctgc ccaaccgccg ttttacgtcg taggttcc 108

<210> 195

<211> 108

<212> DNA

<213> Artificial sequence

<400> 195

gactacatgg gacatgtggc aagaccaggt ctgacctttt ctgggatctg tggcccatca 60

ggtgaggcct gaggtcagcc aggaaggaag cggtacgtcg taggttcc 108

<210> 196

<211> 108

<212> DNA

<213> Artificial sequence

<400> 196

gactacatgg gacatgcccg aggccgcaac gtggtcgtcg tgggagccgg cttcctgggt 60

gagaggtagt gggcagtgga gatggtggtc aggtacgtcg taggttcc 108

<210> 197

<211> 108

<212> DNA

<213> Artificial sequence

<400> 197

gactacatgg gacatggttg ccagtcgcta ggtcagccca tggggccacg gtgtacagtg 60

acaagctgtg gatctttgct ggctatgacg gcatacgtcg taggttcc 108

<210> 198

<211> 108

<212> DNA

<213> Artificial sequence

<400> 198

gactacatgg gacatctgca gctgtcccag tccgccggga gccccacatc ctaccccagc 60

gtcggccggc agggaggccc acctgattcc cactacgtcg taggttcc 108

<210> 199

<211> 108

<212> DNA

<213> Artificial sequence

<400> 199

gactacatgg gacatcaggt caccagagcc ttctttcctg cccacaggac agccactcac 60

tggtgggagc aaccgggtgt ggaggcccgc acctacgtcg taggttcc 108

<210> 200

<211> 108

<212> DNA

<213> Artificial sequence

<400> 200

gactacatgg gacatctcag tgtctgagac tgcgagaggg tcgtaaccat gaggggttcc 60

tgtgagaggt ctggggagga tgaagagcag aaatacgtcg taggttcc 108

<210> 201

<211> 108

<212> DNA

<213> Artificial sequence

<400> 201

gactacatgg gacatgtcat gactgatact aaggactcca tcgctctgcc caaatcatct 60

gccatgtgga aaagacttcc tacattgtgt ccttacgtcg taggttcc 108

<210> 202

<211> 108

<212> DNA

<213> Artificial sequence

<400> 202

gactacatgg gacattcact agtgggagat ccataccaaa tgatgttccc atcatttggg 60

gaggagaaca tcattctttc cttgatggct gaatacgtcg taggttcc 108

<210> 203

<211> 108

<212> DNA

<213> Artificial sequence

<400> 203

gactacatgg gacatcgaag tttgactgcc aaccactcgg agcagcatag gctgactgct 60

gtcggacctc tgtagagagg tagaaggaga ggatacgtcg taggttcc 108

<210> 204

<211> 108

<212> DNA

<213> Artificial sequence

<400> 204

gactacatgg gacatctcca gctgtttatt gtggtcttcc aggatttgca tcctggcttc 60

caggcggcct ttgtgttgac gcagtagctt ggctacgtcg taggttcc 108

<210> 205

<211> 108

<212> DNA

<213> Artificial sequence

<400> 205

gactacatgg gacattgcct ggcatacaac tagtctcata cctgctagca taatgttcaa 60

tgcgtgaatg agtatcatcg tgtgaaagct gagtacgtcg taggttcc 108

<210> 206

<211> 108

<212> DNA

<213> Artificial sequence

<400> 206

gactacatgg gacatctact cacgtttcca tgttgtcccc ctctaagaca gtctgcactg 60

gcaggtagcc cattcgggga tgcttcgcaa aattacgtcg taggttcc 108

<210> 207

<211> 108

<212> DNA

<213> Artificial sequence

<400> 207

gactacatgg gacatatggc cttttgcaac tcgaccagaa aaaaagcagc tttggcagat 60

gtcataatta aagtgcttta gactcctgta ccttacgtcg taggttcc 108

<210> 208

<211> 108

<212> DNA

<213> Artificial sequence

<400> 208

gactacatgg gacatcactt ggctcaatgt tactgccccc aaaggatgca acttcaccca 60

actgtcttgg aatttggata gaatcatgca gaatacgtcg taggttcc 108

<210> 209

<211> 108

<212> DNA

<213> Artificial sequence

<400> 209

gactacatgg gacatcgact tacatctgta cttgtcttcc aaatgtgctt tacacaggga 60

aatgatgcca gttttaaaag acaggacacg gattacgtcg taggttcc 108

<210> 210

<211> 108

<212> DNA

<213> Artificial sequence

<400> 210

gactacatgg gacatacata gtatcaagat cttcaaatac tggccaatac ttacagcaaa 60

gggccttctg cagtcttcgg agtttcatgg cagtacgtcg taggttcc 108

<210> 211

<211> 108

<212> DNA

<213> Artificial sequence

<400> 211

gactacatgg gacatatgca gagtggcata ggaaaggaca aagacaagga atggtttgaa 60

ctgggtactt gcctggaggg atggtcccga gagtacgtcg taggttcc 108

<210> 212

<211> 108

<212> DNA

<213> Artificial sequence

<400> 212

gactacatgg gacatgatac ttccaactta cttgatatag tagggcactt tgtttggcga 60

gatggctctc tcccagggac cctggacaga cgctacgtcg taggttcc 108

<210> 213

<211> 108

<212> DNA

<213> Artificial sequence

<400> 213

gactacatgg gacatataaa gttaagtgat aaaagctgaa aatgacttac tggaaagaaa 60

gtgctgagat gctggaccaa agtccctgtg ggctacgtcg taggttcc 108

<210> 214

<211> 108

<212> DNA

<213> Artificial sequence

<400> 214

gactacatgg gacatagtag acggtacctt gactttctcg aggtgatctt ggagagagtc 60

aatgaggaga tcgcccacgg gctgccagga tcctacgtcg taggttcc 108

<210> 215

<211> 108

<212> DNA

<213> Artificial sequence

<400> 215

gactacatgg gacatcccgg agtctttcaa gggtctcatc tatttttctc tgccagtcag 60

cggagtgcag gttcaatttt tcccactcag tattacgtcg taggttcc 108

<210> 216

<211> 108

<212> DNA

<213> Artificial sequence

<400> 216

gactacatgg gacattctgt cagaaatatt cgtacagtct caagagtact catgattaca 60

ggttctttag ttttcaattc cctcttgaag gcctacgtcg taggttcc 108

<210> 217

<211> 108

<212> DNA

<213> Artificial sequence

<400> 217

gactacatgg gacatgctta attcatcatc tttcagctgt agccacacca gaagttcctg 60

cagagaaagg tgcagacgct tccactggtc agatacgtcg taggttcc 108

<210> 218

<211> 108

<212> DNA

<213> Artificial sequence

<400> 218

gactacatgg gacattgtct tcctgtgtaa cattttcagc ttgaaccggg cactacagca 60

ctgatcctgt tgcatagcaa tcctgagaaa acctacgtcg taggttcc 108

<210> 219

<211> 108

<212> DNA

<213> Artificial sequence

<400> 219

gactacatgg gacattggct actgctataa taagatgctg catacttcac atgctgaatc 60

tggaaggggg agcaattcag tcctatctga cggtacgtcg taggttcc 108

<210> 220

<211> 108

<212> DNA

<213> Artificial sequence

<400> 220

gactacatgg gacatcagct tctgtaagcc aggcaagaaa cttttccagg tccaggggga 60

actgttgcag taatctatga gtttcttcca aagtacgtcg taggttcc 108

<210> 221

<211> 108

<212> DNA

<213> Artificial sequence

<400> 221

gactacatgg gacatagagg cattgatatt ctctgttatc atgtggactt ttctggtatc 60

atctgcagaa taatcccgga gaagtttcag ggctacgtcg taggttcc 108

<210> 222

<211> 108

<212> DNA

<213> Artificial sequence

<400> 222

gactacatgg gacataaatt ttgggcagcg gtaatgagtt cttccaactg gggacgcctc 60

tgttccaaat cctgcattgt tgcctgtaag aactacgtcg taggttcc 108

<210> 223

<211> 108

<212> DNA

<213> Artificial sequence

<400> 223

gactacatgg gacataggag ataaccacag cagcagatga tttaactgct cttcaaggtc 60

ttcaagcttt ttttcaagct gcccaaggtc ttttacgtcg taggttcc 108

<210> 224

<211> 108

<212> DNA

<213> Artificial sequence

<400> 224

gactacatgg gacatgagat ttgtctgctt gagcttattt tcaagtttat cttgctcttc 60

tgggcttatg ggagcactta caagcacggg tcctacgtcg taggttcc 108

<210> 225

<211> 108

<212> DNA

<213> Artificial sequence

<400> 225

gactacatgg gacatttttc caggttcaag tgggatacta gcaatgttat ctgcttcctc 60

caaccataaa acaaattcat ttaaatctct ttgtacgtcg taggttcc 108

<210> 226

<211> 108

<212> DNA

<213> Artificial sequence

<400> 226

gactacatgg gacattcatt tccataatat cctctaaaaa gccttgccta acctagagtg 60

gatgtcctat ttctttatct ttcttgatga tcttacgtcg taggttcc 108

<210> 227

<211> 108

<212> DNA

<213> Artificial sequence

<400> 227

gactacatgg gacatggtac cttatgaaga gaacaaagga aacaatagca tgggatatca 60

gagaaactct agcaacacag tatagctggg gtgtacgtcg taggttcc 108

<210> 228

<211> 108

<212> DNA

<213> Artificial sequence

<400> 228

gactacatgg gacatgcaag gcacagaagc ggggatgtaa acaaaggaga catccgtgaa 60

gatgctaata taggttttca caattctaaa aaatacgtcg taggttcc 108

<210> 229

<211> 108

<212> DNA

<213> Artificial sequence

<400> 229

gactacatgg gacattccca attctcagga atttgtgtct ttctgagaaa ctgttcagct 60

tctgttagcc actgattaaa tatctttata tcatacgtcg taggttcc 108

<210> 230

<211> 108

<212> DNA

<213> Artificial sequence

<400> 230

gactacatgg gacatatcaa gctgggagag agcttcctgt agcttcaccc tttccacagg 60

cgttgcactt tgcaatgctg ctgtcttctt gcttacgtcg taggttcc 108

<210> 231

<211> 108

<212> DNA

<213> Artificial sequence

<400> 231

gactacatgg gacatctcag acaagccctc agcttgccta cgcactgcat tcagctcctc 60

tttcttcttc tgcaattccc gatcaatttc ctatacgtcg taggttcc 108

<210> 232

<211> 108

<212> DNA

<213> Artificial sequence

<400> 232

gactacatgg gacatttacc atatctttat tgaagtcttc ctctttcaga ttcaccccct 60

gctgaatttc agcctccagt ggttcaagca atttacgtcg taggttcc 108

<210> 233

<211> 108

<212> DNA

<213> Artificial sequence

<400> 233

gactacatgg gacatgtgaa atggctgcaa atcgatggtt gagctctgag atttggggct 60

ctactaattt cctgcagtgg tcaccgcggt ttgtacgtcg taggttcc 108

<210> 234

<211> 108

<212> DNA

<213> Artificial sequence

<400> 234

gactacatgg gacatgctgg ggtttctttt tctctgattc atccaaaagt gtgtcagcct 60

gaatgatcca ctttgtgatg tggtccacat tcttacgtcg taggttcc 108

<210> 235

<211> 108

<212> DNA

<213> Artificial sequence

<400> 235

gactacatgg gacatttcca cactctttgt ttccaatgca ggcaagtgca tcttcacttc 60

atctaaaatc atcttacttt cttgtagacg ctgtacgtcg taggttcc 108

<210> 236

<211> 108

<212> DNA

<213> Artificial sequence

<400> 236

gactacatgg gacatctcaa gttcctcatt gattagctca tccatgactc cgccatctgt 60

tagggtctgt gccaatatgc gaatctgatt tggtacgtcg taggttcc 108

<210> 237

<211> 108

<212> DNA

<213> Artificial sequence

<400> 237

gactacatgg gacataaatt ctacttcatt tagccacttg tttgctttct ccaagtatga 60

caataactca tgccaacatg cccaaacttc ctatacgtcg taggttcc 108

<210> 238

<211> 108

<212> DNA

<213> Artificial sequence

<400> 238

gactacatgg gacatcgtgc atctctgata gatctttctg gaggcttaca gttttctcca 60

aacctccctt caaggcctcc tttctggcat agatacgtcg taggttcc 108

<210> 239

<211> 108

<212> DNA

<213> Artificial sequence

<400> 239

gactacatgg gacatgagtt tattcatttg ctcctctagc ttttgacaat gctcaaccag 60

ctgggaggag agcttcttcc agcgtccctc aattacgtcg taggttcc 108

<210> 240

<211> 108

<212> DNA

<213> Artificial sequence

<400> 240

gactacatgg gacattcttt cacagtggtg ctgagatagt ataggccact ttgttgctct 60

tgcagagaac tttgtaaagc ctaaaaaaca atttacgtcg taggttcc 108

<210> 241

<211> 108

<212> DNA

<213> Artificial sequence

<400> 241

gactacatgg gacatctgag ctgatctgct ggcatcttgc agttttctga acttctcagc 60

tttttctcgc tctatggcct gcagcatgag agctacgtcg taggttcc 108

<210> 242

<211> 108

<212> DNA

<213> Artificial sequence

<400> 242

gactacatgg gacatgtggt ggaagttcct cttgagcatg ctttaccagg atctgttccc 60

ttgtggtcac cgtagttact gtttccatta cagtacgtcg taggttcc 108

<210> 243

<211> 108

<212> DNA

<213> Artificial sequence

<400> 243

gactacatgg gacataactg ggtgccttgt catctcaatc acgatttcca caccctgcct 60

cacaataggt tttaaccatt atgagaactt tgctacgtcg taggttcc 108

<210> 244

<211> 108

<212> DNA

<213> Artificial sequence

<400> 244

gactacatgg gacattgaca cacctgttct tcagtaagac gttgccattt gagaaggatg 60

tcttgtaaaa gaacccagcg gtcttctgtc cattacgtcg taggttcc 108

<210> 245

<211> 108

<212> DNA

<213> Artificial sequence

<400> 245

gactacatgg gacatctacc ttatgttgtt gtacttggcg ttttaggtct tcaagatcag 60

gtccaagagg ctcttcctcc attttccttg ttctacgtcg taggttcc 108

<210> 246

<211> 108

<212> DNA

<213> Artificial sequence

<400> 246

gactacatgg gacatagcta ccctgaggca ttcccatctt gaatttagga gattcatctg 60

ctcttgtact tcagtttctt catcttctga taatacgtcg taggttcc 108

<210> 247

<211> 108

<212> DNA

<213> Artificial sequence

<400> 247

gactacatgg gacatatgag agcattcaaa gccaggccat cagaccagct ggtggtgaag 60

ttgattacat taacctgtgg ataattacga gtttacgtcg taggttcc 108

<210> 248

<211> 108

<212> DNA

<213> Artificial sequence

<400> 248

gactacatgg gacatcagtg ccttgttgac attgttcagg gcatgaactc ttgtggatcc 60

tttttctttt ggctgagaac aaaacaaaag agttacgtcg taggttcc 108

<210> 249

<211> 108

<212> DNA

<213> Artificial sequence

<400> 249

gactacatgg gacatcctcc tgatcttgcg gtctctcggt catttgttca tgtcccttaa 60

tgacatattg tcattatctg acttgaagtt gtttacgtcg taggttcc 108

<210> 250

<211> 108

<212> DNA

<213> Artificial sequence

<400> 250

gactacatgg gacatcccat tttgtgaatg ttttcttttg aacatcttct ctttcatcta 60

aaatgcaaaa taaaaaaata aaagttagga agctacgtcg taggttcc 108

<210> 251

<211> 108

<212> DNA

<213> Artificial sequence

<400> 251

gactacatgg gacatagggt ctatccgagc agagctggag ggcctgagag gtgggtaaag 60

ggagaaatta gatcaccttc ctggtcgttt ttatacgtcg taggttcc 108

<210> 252

<211> 108

<212> DNA

<213> Artificial sequence

<400> 252

gactacatgg gacatcaaaa gttctctcta gctcagaggt cactcagaca ttcacaaggt 60

gagaaggatg tggtctgccg tcactgtgag tgctacgtcg taggttcc 108

<210> 253

<211> 108

<212> DNA

<213> Artificial sequence

<400> 253

gactacatgg gacatggtga gttctcaatg agaaggaagc actgttgcag gaaaagaaat 60

ggaaaggaca gacgtgactc ttggacagat cactacgtcg taggttcc 108

<210> 254

<211> 108

<212> DNA

<213> Artificial sequence

<400> 254

gactacatgg gacattctcg gggacacatt ataaagtaac actccttcat cccactggaa 60

tctcttccaa atctaaacat ccaggctcca cagtacgtcg taggttcc 108

<210> 255

<211> 108

<212> DNA

<213> Artificial sequence

<400> 255

gactacatgg gacatactga gtaggatata cttaacaaat ccctcttttg gctaagctat 60

ctaacttcaa aggccaactg gcctttgaag agttacgtcg taggttcc 108

<210> 256

<211> 108

<212> DNA

<213> Artificial sequence

<400> 256

gactacatgg gacattcaaa tcagaaacga acaggcactc acctcgaagt cacactcttc 60

tcccacagca tatttggtca tgatctccaa ccatacgtcg taggttcc 108

<210> 257

<211> 108

<212> DNA

<213> Artificial sequence

<400> 257

gactacatgg gacatatggc tgctctcaga taaaggcaga gtccatacag ttggtgctag 60

gaggttccct ggagccagag aggacagcag agatacgtcg taggttcc 108

<210> 258

<211> 108

<212> DNA

<213> Artificial sequence

<400> 258

gactacatgg gacatgaggc tccgtcttgt cccacctgca gtcagggctg aagcgcacca 60

gggtggcgtg gtccagctcc acgttggctc tcatacgtcg taggttcc 108

<210> 259

<211> 108

<212> DNA

<213> Artificial sequence

<400> 259

gactacatgg gacatggccc tgtggttttg ggggtgccag cctgggcccg gggctcacct 60

tgaggctggg ctgggcactg agtgtgctca cgatacgtcg taggttcc 108

<210> 260

<211> 108

<212> DNA

<213> Artificial sequence

<400> 260

gactacatgg gacatagctg ccgcccaccc ggctcctcct ctgggtcccc cagcagcacg 60

gggaccacac tggagaagag ctgtttgcac cattacgtcg taggttcc 108

<210> 261

<211> 108

<212> DNA

<213> Artificial sequence

<400> 261

gactacatgg gacatgctag gcacaggcca agtcttctgc ctccaggaag ctgagccagg 60

aggccaagac attcagggtc ttagaccccc tgctacgtcg taggttcc 108

<210> 262

<211> 108

<212> DNA

<213> Artificial sequence

<400> 262

gactacatgg gacattgggt gagggcacgt ctgggagaac cccctggggc tggtgggggc 60

agttggccat ccggtgggtt ggccttggga agttacgtcg taggttcc 108

<210> 263

<211> 108

<212> DNA

<213> Artificial sequence

<400> 263

gactacatgg gacatatgat tgatatccag accaggatgg ctgggcgagc attggagctt 60

ctttatctgc cagagaataa gccctgttac ctgtacgtcg taggttcc 108

<210> 264

<211> 108

<212> DNA

<213> Artificial sequence

<400> 264

gactacatgg gacatttgta agacagtgat gttcctgttt ctttcaggtc cggggatccc 60

gagctgtcct gcagctgtac cctgagaact cagtacgtcg taggttcc 108

<210> 265

<211> 108

<212> DNA

<213> Artificial sequence

<400> 265

gactacatgg gacatgggcc tgaaacccgt cccccgcccc acacactcac tctcatcggg 60

gttgggggat gccaggatgg cactgtgctt ccgtacgtcg taggttcc 108

<210> 266

<211> 108

<212> DNA

<213> Artificial sequence

<400> 266

gactacatgg gacatgacca ggaagcctcg gatggcagct atgaagtcct gtgggcggtc 60

agcgtggatc cagtggccag cgttcggcac cgttacgtcg taggttcc 108

<210> 267

<211> 108

<212> DNA

<213> Artificial sequence

<400> 267

gactacatgg gacatccggc acgagggtga gcagggcggc gagaaggaac agcacgcctg 60

ccacgatggt gatcttggcc ttggccgtgt cgttacgtcg taggttcc 108

<210> 268

<211> 108

<212> DNA

<213> Artificial sequence

<400> 268

gactacatgg gacatgccca gagggcaggg tctgagccct ctgcccagag cagaacaaag 60

cgctgagcaa gaacacagca ggagacggga aactacgtcg taggttcc 108

<210> 269

<211> 108

<212> DNA

<213> Artificial sequence

<400> 269

gactacatgg gacattgatg gctgacggtt gtctctctct cttcttctgc agttggttca 60

gaaccgagat cacctctata atttcctgct ccttacgtcg taggttcc 108

<210> 270

<211> 108

<212> DNA

<213> Artificial sequence

<400> 270

gactacatgg gacatttcgt agcccctaaa ccttctccat ttcctcattg caaagtgggg 60

attaaaacct cgggctcccc aggccgtcag gtttacgtcg taggttcc 108

<210> 271

<211> 108

<212> DNA

<213> Artificial sequence

<400> 271

gactacatgg gacatcagcg agggagctgg ggagggagga gcctacccag ctgggaatgg 60

gacaaggaaa ctaggaacag gacaaggaag ccatacgtcg taggttcc 108

<210> 272

<211> 108

<212> DNA

<213> Artificial sequence

<400> 272

gactacatgg gacatctgct aaggcagctg caaaggcagc caaatacggt gagtgctatg 60

ctgacagctc tgccccaccc tgtcctggcc ttttacgtcg taggttcc 108

<210> 273

<211> 108

<212> DNA

<213> Artificial sequence

<400> 273

gactacatgg gacattgact ctcccttcat cccatgttcc ttgatggagc tgctcttagc 60

ctttccccga aaggcttctt ggagcagtgg agctacgtcg taggttcc 108

<210> 274

<211> 108

<212> DNA

<213> Artificial sequence

<400> 274

gactacatgg gacatgaggc cttcgggggg caagagggct ctcaacaggc agaggaaacc 60

atctgcacag cggtgggatg gtgcggactg ctgtacgtcg taggttcc 108

<210> 275

<211> 108

<212> DNA

<213> Artificial sequence

<400> 275

gactacatgg gacatcctct cctgcaggtg ttgtgactgc agtgcctccc tgtcgcacca 60

gtactatgag aaggatgggc agctcttctg caatacgtcg taggttcc 108

<210> 276

<211> 108

<212> DNA

<213> Artificial sequence

<400> 276

gactacatgg gacattcaag gtacagagca tgccagggtc tcaggggaca gtctgggtgg 60

gacccctcca tcctccttcc ttcccagtct atgtacgtcg taggttcc 108

<210> 277

<211> 108

<212> DNA

<213> Artificial sequence

<400> 277

gactacatgg gacatcgtgt gaggcaggga tttggcggct ctggcccggg gatggtacga 60

ctccttgcgg gcggggggag accaaggaga taatacgtcg taggttcc 108

<210> 278

<211> 108

<212> DNA

<213> Artificial sequence

<400> 278

gactacatgg gacatatatc agttacactt gtgaagtaag ctccaggaag cattaggaac 60

taaactttgg gttggagtgt gtgggtgagc ctatacgtcg taggttcc 108

<210> 279

<211> 108

<212> DNA

<213> Artificial sequence

<400> 279

gactacatgg gacatgtggg gacttggcgt tctacggcac acagagttgt cggcatagat 60

ccccacgttc tctggaatag caagttcacc ttgtacgtcg taggttcc 108

<210> 280

<211> 108

<212> DNA

<213> Artificial sequence

<400> 280

gactacatgg gacatcctgc cttcattcac cctcatgccc cagaatctca gggctacctc 60

cacagccctg tccacagacc cctcctgacc cgttacgtcg taggttcc 108

<210> 281

<211> 108

<212> DNA

<213> Artificial sequence

<400> 281

gactacatgg gacatccgga agaagatgag gaatctgagg attatcagaa ctcagcatcc 60

atccatcagt ggcgcgagtc caggaaggtc atgtacgtcg taggttcc 108

<210> 282

<211> 108

<212> DNA

<213> Artificial sequence

<400> 282

gactacatgg gacataccca cactagcgct tgtaccttga acacgttctc actgttaatg 60

aagccaaatc ctgagaaggt ggactgcagg ttgtacgtcg taggttcc 108

<210> 283

<211> 108

<212> DNA

<213> Artificial sequence

<400> 283

gactacatgg gacatctggg ctgcaaggct agggccgtca caacagaacc agtaatacaa 60

aactcatgtt tgcagaggaa atgcttcaaa atgtacgtcg taggttcc 108

<210> 284

<211> 108

<212> DNA

<213> Artificial sequence

<400> 284

gactacatgg gacatctgtg ggtggctcca gggcaggcag gccaattgcc agggaggacc 60

ctctagggga ccatccatcc ctccactgta cactacgtcg taggttcc 108

<210> 285

<211> 108

<212> DNA

<213> Artificial sequence

<400> 285

gactacatgg gacatgagca gcatttgagg gggacctttg agagggtctc tcacgctagg 60

aggtggtgca gggctacaag ggagaggtgg tcatacgtcg taggttcc 108

<210> 286

<211> 108

<212> DNA

<213> Artificial sequence

<400> 286

gactacatgg gacatcacat cattcgaggt ggagccctcc ctcccgctcc cccgggtacc 60

acattttggg ttccttcctc agttacgcac atgtacgtcg taggttcc 108

<210> 287

<211> 108

<212> DNA

<213> Artificial sequence

<400> 287

gactacatgg gacatccggc cgcagctgct gcccccgaga agccgggccc caaggcggcg 60

gaagtggggg atgacttcct gggggacttt gtgtacgtcg taggttcc 108

<210> 288

<211> 108

<212> DNA

<213> Artificial sequence

<400> 288

gactacatgg gacatgccat gacaaagtgt tggagttcct gggaatgtcc tgtctctatg 60

taggcagacc tagaaaccac tgatccatgt ctctacgtcg taggttcc 108

<210> 289

<211> 108

<212> DNA

<213> Artificial sequence

<400> 289

gactacatgg gacatagaag cggcgcctgg aggcagagct ggagaccgtg tcccggaaga 60

cccatgacgc ctcgggccag ctagtcctca tcatacgtcg taggttcc 108

<210> 290

<211> 108

<212> DNA

<213> Artificial sequence

<400> 290

gactacatgg gacatggggt tggaatttgg agcctgagac ggtcccatgg gaagacctcg 60

ccatctagtg ggaaaatcgg gaactgctgc ctgtacgtcg taggttcc 108

<210> 291

<211> 108

<212> DNA

<213> Artificial sequence

<400> 291

gactacatgg gacatcttgg ggtagaagtt tcatgtttgc ctgaaataag agcaggactt 60

ctgagggtag ggaaggaagg tggtgaagcc acttacgtcg taggttcc 108

<210> 292

<211> 108

<212> DNA

<213> Artificial sequence

<400> 292

gactacatgg gacataggtg ctgcagtggg gcccccctct ttcccttcct tggacatcct 60

aacccctctt ggggtttccc gagcttcttg gattacgtcg taggttcc 108

<210> 293

<211> 108

<212> DNA

<213> Artificial sequence

<400> 293

gactacatgg gacatgttgg gccctgtgcg gtgtgggaca cagtgtgaga gagtattggt 60

gcctgtgagc tggtggctgt gtccccaccc ctgtacgtcg taggttcc 108

<210> 294

<211> 108

<212> DNA

<213> Artificial sequence

<400> 294

gactacatgg gacatgctag cctcctaact ggccctgagc gccaggtgca gggtgtttgg 60

gattggaaat cacagctgga tttctgttag ttctacgtcg taggttcc 108

<210> 295

<211> 108

<212> DNA

<213> Artificial sequence

<400> 295

gactacatgg gacataacgg ctgcggaccc gaccgctgga actccgcgtt cacccgcaaa 60

gacgagatca tcaccagcct cgtgtctgcc ttatacgtcg taggttcc 108

<210> 296

<211> 108

<212> DNA

<213> Artificial sequence

<400> 296

gactacatgg gacatgctat ttatagcacc aggaactcgg ctggtttatt tcaagctccg 60

cagatcccag aggagaatag gctccaagct gggtacgtcg taggttcc 108

<210> 297

<211> 108

<212> DNA

<213> Artificial sequence

<400> 297

gactacatgg gacatccaaa atgccaagcc ttttccaaat gtcagttcct gagtcacatc 60

caaagagtgt tgggtgtgga gtggtcaaga tcttacgtcg taggttcc 108

<210> 298

<211> 108

<212> DNA

<213> Artificial sequence

<400> 298

gactacatgg gacatactgt tctcatgcct gtctctcttc tctccccagg atgtttgatg 60

agcgaatttt cacaggtatg tggggaccat ctatacgtcg taggttcc 108

<210> 299

<211> 108

<212> DNA

<213> Artificial sequence

<400> 299

gactacatgg gacatctttg ccattgacag gagacatctt tcctcactgt gacttcctgt 60

gccctctagg tgaggccctg ggcctgaacc ggctacgtcg taggttcc 108

<210> 300

<211> 108

<212> DNA

<213> Artificial sequence

<400> 300

gactacatgg gacatagaac acccttctgc ttaagctgag agaattctaa tgacttattt 60

tgtggttttg gatctagcta tatgtggagc cagtacgtcg taggttcc 108

<210> 301

<211> 108

<212> DNA

<213> Artificial sequence

<400> 301

gactacatgg gacatctagc tgaggggtca gggctcacca ggctgacagc tctattgggg 60

gctgatcaac tgctcatctc tcctccgtga tgttacgtcg taggttcc 108

<210> 302

<211> 108

<212> DNA

<213> Artificial sequence

<400> 302

gactacatgg gacattctta atagatcctg ggtttgagca gtttaaaatg tcagaaagat 60

tgcatgggaa ctggatcaga ctgtacttgg aggtacgtcg taggttcc 108

<210> 303

<211> 108

<212> DNA

<213> Artificial sequence

<400> 303

gactacatgg gacatccatc tcatttcagc tctgaccaca aagccgagct ctctgtcctg 60

gctgccctct ccctcctcgt agtttttgtg ctgtacgtcg taggttcc 108

<210> 304

<211> 108

<212> DNA

<213> Artificial sequence

<400> 304

gactacatgg gacatgaaat tgtcttctac aaagtgatcg actacgtcct ccacggcaag 60

gaggagatca aggtcattcc gtaagtgcag gattacgtcg taggttcc 108

<210> 305

<211> 108

<212> DNA

<213> Artificial sequence

<400> 305

gactacatgg gacattctcg tgacacatct gtgtctctgt gtagccaacc agagcgcgcc 60

tggggaagga gcctgctgac tgcgatgagg acgtacgtcg taggttcc 108

<210> 306

<211> 108

<212> DNA

<213> Artificial sequence

<400> 306

gactacatgg gacatacctg aaccggcggc agcacgagca cgtgatccac ctgatggaca 60

tcgccatcat cgccacggac ctggccctgt acttacgtcg taggttcc 108

<210> 307

<211> 108

<212> DNA

<213> Artificial sequence

<400> 307

gactacatgg gacatccatg gggaccctat ggctccctca atcttcaccc actaggattt 60

gggttctgcc tgtggctatt tgctacaaga ggttacgtcg taggttcc 108

<210> 308

<211> 108

<212> DNA

<213> Artificial sequence

<400> 308

gactacatgg gacataattc gctcccggag cccgcaagga gccctttcgc ccccgcccgc 60

aggtggacca gatgttccag ttcgcctcca tcgtacgtcg taggttcc 108

<210> 309

<211> 108

<212> DNA

<213> Artificial sequence

<400> 309

gactacatgg gacataccac acagaccagg cacgggcgtc tcatgacccc tcaccattaa 60

cggaatgtcc tcacccttct tgaccagcac aggtacgtcg taggttcc 108

<210> 310

<211> 108

<212> DNA

<213> Artificial sequence

<400> 310

gactacatgg gacattgggc tgggcaccca tgaggctgag gaccctgggg agggaacgct 60

gcttgtcggg cggtggtgac gatttcatgg atgtacgtcg taggttcc 108

<210> 311

<211> 108

<212> DNA

<213> Artificial sequence

<400> 311

gactacatgg gacattgctt tgatgacagt ctgcaggagc tgcctggtga agtacctgga 60

ggagaacaac acctgcccca cctgcaggat tgttacgtcg taggttcc 108

<210> 312

<211> 108

<212> DNA

<213> Artificial sequence

<400> 312

gactacatgg gacattaggg taaagcatgg cagatataaa acatggcagg tcagtggccg 60

gctctggcct cagccccatg gcccacgttt tgttacgtcg taggttcc 108

<210> 313

<211> 108

<212> DNA

<213> Artificial sequence

<400> 313

gactacatgg gacattccca cacttgacct tgggagtttg gaaactggcg aagccgcctc 60

ttccactgcc cttgctgttc cctcctgagg tcctacgtcg taggttcc 108

<210> 314

<211> 108

<212> DNA

<213> Artificial sequence

<400> 314

gactacatgg gacatacccc agagccagct tggaaggtgc cagctgagct gcgaagggca 60

caggccctgg tgggaaagtg ctgaggccat gagtacgtcg taggttcc 108

<210> 315

<211> 108

<212> DNA

<213> Artificial sequence

<400> 315

gactacatgg gacattcagc ttgagtgggt ccgtgtcttt agccaggtcc tgcttcctca 60

tctctgcaat ggtctttggc cctttcttgt cagtacgtcg taggttcc 108

<210> 316

<211> 108

<212> DNA

<213> Artificial sequence

<400> 316

gactacatgg gacattctgt ggctggcgtg ggggccactg ccgatgctgc agtctcggtc 60

caggcagccc atcctcccag caggtctggg ttgtacgtcg taggttcc 108

<210> 317

<211> 108

<212> DNA

<213> Artificial sequence

<400> 317

gactacatgg gacatacatg cctgtgatgt gtgaaaggcc tctagttcac cccttcctgc 60

tcaccctcta aaacatcctc tccctgcacg aggtacgtcg taggttcc 108

<210> 318

<211> 108

<212> DNA

<213> Artificial sequence

<400> 318

gactacatgg gacatacggc ggcctccgcg gtgctgaaga gacggcagaa gcacaggaag 60

gagcagacgg ccacagcaga cgcggctctc ccgtacgtcg taggttcc 108

<210> 319

<211> 108

<212> DNA

<213> Artificial sequence

<400> 319

gactacatgg gacatgtcgc cagctgtgga aggaacgtgg gcccaccagg ggccgcagtt 60

cctgcatcac gggcaccagt ccagcccagc tcctacgtcg taggttcc 108

<210> 320

<211> 108

<212> DNA

<213> Artificial sequence

<400> 320

gactacatgg gacatgcgtg tcgtgcgggg ggatcgagta cttcccattg actattcgaa 60

gtttcgctcc atcctcaaaa gggtgctgcc ggatacgtcg taggttcc 108

<210> 321

<211> 108

<212> DNA

<213> Artificial sequence

<400> 321

gactacatgg gacatgcggc acgtctggta gaagatcttc agaaccgtgt cgcacgaaag 60

ggggcctcga gattccattt tcttcaaaaa ttctacgtcg taggttcc 108

<210> 322

<211> 108

<212> DNA

<213> Artificial sequence

<400> 322

gactacatgg gacatcgccg tctctgtctc ctcactcccc gccgtttgtt gctgcctcag 60

cttctgtttt ccccacgtct ttgccgtcct gtctacgtcg taggttcc 108

<210> 323

<211> 108

<212> DNA

<213> Artificial sequence

<400> 323

gactacatgg gacattcgtt aatggtgacc ttccctgatg tgcctctggg catcttcttg 60

ttctgtgtgt gtgtgatcgc cattggggtc gtgtacgtcg taggttcc 108

<210> 324

<211> 108

<212> DNA

<213> Artificial sequence

<400> 324

gactacatgg gacatggcgc tgctgcacca ggcggagacg ctgcagccct cggtgtggtt 60

tggcggccgg gagcatgtgc tcatgttcgc caatacgtcg taggttcc 108

<210> 325

<211> 108

<212> DNA

<213> Artificial sequence

<400> 325

gactacatgg gacatcttgg gaggagtaga tgtgactcac ggacaccacg gtggctgcaa 60

aggcaggctg tggtcagggc ggtgggaggc gaatacgtcg taggttcc 108

<210> 326

<211> 108

<212> DNA

<213> Artificial sequence

<400> 326

gactacatgg gacattcgcc agccacgctc gagccaaagc gcttgtggag ctgcccgaag 60

tgcgacacga gtgtggccac cacgatgacc agctacgtcg taggttcc 108

<210> 327

<211> 108

<212> DNA

<213> Artificial sequence

<400> 327

gactacatgg gacatcgaca atgccttcct gagctaccac ccgcacccct tcgcgcagcg 60

cacgctcacc gcgcgcttcc aggtcaacaa cactacgtcg taggttcc 108

<210> 328

<211> 108

<212> DNA

<213> Artificial sequence

<400> 328

gactacatgg gacatccgtg cccgccagga cgatgcgggc atgtacatct gccttggcgc 60

caacaccatg ggctacagct tccgcagcgc ctttacgtcg taggttcc 108

<210> 329

<211> 108

<212> DNA

<213> Artificial sequence

<400> 329

gactacatgg gacatggggc tgatgagtga ggtggcagca ggcatccgtg tggttttgct 60

ggaagagtga tgacgaaaat gccagcgtca gtgtacgtcg taggttcc 108

<210> 330

<211> 108

<212> DNA

<213> Artificial sequence

<400> 330

gactacatgg gacatcgtgc ccgcagttgg tgaggctgaa gcacgacgtc ctgtggggcg 60

gctggaagca gcgattacag aacacccagt tggtacgtcg taggttcc 108

<210> 331

<211> 108

<212> DNA

<213> Artificial sequence

<400> 331

gactacatgg gacatgtggc ggctgtcaga tggtccttgt tgacacagtt cttcaggctg 60

tctgggatcc ggcctgggag atgcaggagg aagtacgtcg taggttcc 108

<210> 332

<211> 108

<212> DNA

<213> Artificial sequence

<400> 332

gactacatgg gacatccggc cgacttgatg tcgatgttgt caaaaccact gccaatccgg 60

acgatgatgc ggagggcttt gaacttctcc aggtacgtcg taggttcc 108

<210> 333

<211> 108

<212> DNA

<213> Artificial sequence

<400> 333

gactacatgg gacatgcggt ggaatccatc caggccgagg acgagagcgc caagctgtgc 60

aagcgccgga tcgagcacct caaagagcat agctacgtcg taggttcc 108

<210> 334

<211> 108

<212> DNA

<213> Artificial sequence

<400> 334

gactacatgg gacatacggt tttgtttccc ctgtgtcctg tggatgcata ccgtgctgtt 60

ctccccggac ctgctctgag ggccccacat ctctacgtcg taggttcc 108

<210> 335

<211> 108

<212> DNA

<213> Artificial sequence

<400> 335

gactacatgg gacatcactt ctcacaagcc gagaaggtgt acatcatgta ggccccacgt 60

cgtgaagcgc acgcctcggg gacgggctgc agttacgtcg taggttcc 108

<210> 336

<211> 108

<212> DNA

<213> Artificial sequence

<400> 336

gactacatgg gacatgcgga gagggagatg caaggcaagt gtccaggacg gagggagggg 60

cccacgcctc tgagggttgc ccgtggtcca gcatacgtcg taggttcc 108

<210> 337

<211> 108

<212> DNA

<213> Artificial sequence

<400> 337

gactacatgg gacatgcatc gttctcttta gtaccgtcag cgtggctctg tctttccttc 60

tgctagttac cgggttactt gtttccgtgg ctgtacgtcg taggttcc 108

<210> 338

<211> 108

<212> DNA

<213> Artificial sequence

<400> 338

gactacatgg gacattcatc caggtacagc gggaaaggca gggggaagaa gaggaggtac 60

cccagcctca ccaacctgaa ggctcaggct gattacgtcg taggttcc 108

<210> 339

<211> 108

<212> DNA

<213> Artificial sequence

<400> 339

gactacatgg gacatagctc acgccgaggc ctcggacgcg ggactcgcac tcgcgggcac 60

agctgtgcgg ctagagcgtg aaaacagcag gcttacgtcg taggttcc 108

<210> 340

<211> 108

<212> DNA

<213> Artificial sequence

<400> 340

gactacatgg gacatgagtg tgaccatggt cggggccccg tgtcctccgt ccacaccaac 60

aggcactgga gtcctggaac atcagacttg cgatacgtcg taggttcc 108

<210> 341

<211> 108

<212> DNA

<213> Artificial sequence

<400> 341

gactacatgg gacattcgta ggcaattgtg ttcttcccat agttgatctg cttctgtctc 60

ctcattagga cactttcatc tgtctcaaag tcatacgtcg taggttcc 108

<210> 342

<211> 108

<212> DNA

<213> Artificial sequence

<400> 342

gactacatgg gacatgagcc gcttttctga agccgcaagg cacatgccca catagtagcc 60

tgcaaaggac agggtgagcc tcaggggaaa gtgtacgtcg taggttcc 108

<210> 343

<211> 108

<212> DNA

<213> Artificial sequence

<400> 343

gactacatgg gacatgcagg agaggacccc cccatgccag cttctcgggg ctcttaccac 60

ctcgactggg acaaaatgga tgacccaaac ttctacgtcg taggttcc 108

<210> 344

<211> 108

<212> DNA

<213> Artificial sequence

<400> 344

gactacatgg gacatgcgac acaggaggag aaccgggagc tgaggagcag gtgtgaggag 60

ctccacggga agaacctgga actggggtaa ggatacgtcg taggttcc 108

<210> 345

<211> 108

<212> DNA

<213> Artificial sequence

<400> 345

gactacatgg gacataatgg cagcaaggtg ggcccggacg gcacacccta cgttaccgtg 60

ctcaaggtgg gccaccgtgt gcacgtgggt gcctacgtcg taggttcc 108

<210> 346

<211> 108

<212> DNA

<213> Artificial sequence

<400> 346

gactacatgg gacattgact gcaggactgt gtcaggcatt tctggctgtg gctcggtccc 60

cggcctttgg ggagcagcta ccacacgttc gggtacgtcg taggttcc 108

<210> 347

<211> 108

<212> DNA

<213> Artificial sequence

<400> 347

gactacatgg gacatagagt gcacgccagt atcacgtaca gttctttggt gacgccccag 60

aaagagcttg gatatttgag aagagcctcg tagtacgtcg taggttcc 108

<210> 348

<211> 108

<212> DNA

<213> Artificial sequence

<400> 348

gactacatgg gacatatctg tctgatgcat gtaaaccact gaagaagcga aatcgggctt 60

ccacggcagc atcttcagct cttgggttta gcatacgtcg taggttcc 108

<210> 349

<211> 108

<212> DNA

<213> Artificial sequence

<400> 349

gactacatgg gacatccgta catggagggg gaccggggca gccgctacca gggggtcaga 60

gggatcggaa gagtcttcaa aaacggtacg gagtacgtcg taggttcc 108

<210> 350

<211> 108

<212> DNA

<213> Artificial sequence

<400> 350

gactacatgg gacatgaccg tataatagac gctggcccca aaggaaacta ctctcgattt 60

atgaatcaca gctgccagcc caactgtgag acctacgtcg taggttcc 108

<210> 351

<211> 108

<212> DNA

<213> Artificial sequence

<400> 351

gactacatgg gacattagac ttaacacctg tgattgaaga attgacgaat gcaggctttc 60

tacagacagg tatgactagt agaaggagat gtgtacgtcg taggttcc 108

<210> 352

<211> 108

<212> DNA

<213> Artificial sequence

<400> 352

gactacatgg gacattcatt gcagctggat tttttgtgaa tctaatgagg tttctttgtt 60

attgttgcaa taggaagccg tcagagaact tgatacgtcg taggttcc 108

<210> 353

<211> 108

<212> DNA

<213> Artificial sequence

<400> 353

gactacatgg gacatcggat gtcttcagaa acgcctgtca ggtactccag tgcccctgcc 60

ccacgagtag gtccttctgc acacatccgt ggctacgtcg taggttcc 108

<210> 354

<211> 108

<212> DNA

<213> Artificial sequence

<400> 354

gactacatgg gacatgtctc agtagacaac catattttgg ccccacttaa aaatttgctt 60

aatggtgttc ctaaaatgct tcgtctgcac agatacgtcg taggttcc 108

<210> 355

<211> 108

<212> DNA

<213> Artificial sequence

<400> 355

gactacatgg gacatagatt ttgttacctc tttctctgat ctctttagat ggttgcatct 60

gtctagaaac tgatatcctg ccatgaccca ctctacgtcg taggttcc 108

<210> 356

<211> 108

<212> DNA

<213> Artificial sequence

<400> 356

gactacatgg gacatatggt taaggacacc aggcaaacaa aagaaaaccc cacaaatcta 60

ccaggagctt tagctctctt cttaagtccc tcttacgtcg taggttcc 108

<210> 357

<211> 108

<212> DNA

<213> Artificial sequence

<400> 357

gactacatgg gacataaaag gactggctcc agttttttaa tcttcggttc cgaattgatc 60

ttatcgtcag tctgaaatac attagcaaaa taatacgtcg taggttcc 108

<210> 358

<211> 108

<212> DNA

<213> Artificial sequence

<400> 358

gactacatgg gacatcccat agagtggggc gcccgagtca gcaaaccctt ggctcacggg 60

cccgcggtgg ggacgctaca cgtacctttc agatacgtcg taggttcc 108

<210> 359

<211> 108

<212> DNA

<213> Artificial sequence

<400> 359

gactacatgg gacatatggc ttgacgggct actccgaaac tcatgagcac gaccagcatg 60

atgaccacaa agtacagcat gtcgatcatc tgatacgtcg taggttcc 108

<210> 360

<211> 108

<212> DNA

<213> Artificial sequence

<400> 360

gactacatgg gacatcttcc catccacata tcggtcagca gcatctggct gcaggtgtga 60

gcaatgaagt cccggtgttt ggctgccacg gcctacgtcg taggttcc 108

<210> 361

<211> 108

<212> DNA

<213> Artificial sequence

<400> 361

gactacatgg gacataggga gaacatacta ataggtcact tgtacaaagt caagatcaat 60

gatatgaatc gaaaaaaaac agtttcaccg gcctacgtcg taggttcc 108

<210> 362

<211> 108

<212> DNA

<213> Artificial sequence

<400> 362

gactacatgg gacatatcgt tggtctcagc tctgaagggc ttcagataaa aatctgccag 60

cattggttaa gagaagattg gagaatttca aattacgtcg taggttcc 108

<210> 363

<211> 108

<212> DNA

<213> Artificial sequence

<400> 363

gactacatgg gacatattat cgggagggaa agggcctgct cttacctgtg ctgacacccc 60

cggtgaagat ccaggccccg gtggtcatag cagtacgtcg taggttcc 108

<210> 364

<211> 108

<212> DNA

<213> Artificial sequence

<400> 364

gactacatgg gacatgtatc aaacacctga acgttctttc tgctgggggg atgcgtcaga 60

ggaggccttt gtaccggggc gatgcgataa gcctacgtcg taggttcc 108

<210> 365

<211> 108

<212> DNA

<213> Artificial sequence

<400> 365

gactacatgg gacatctccg aaaagcacag cagttgggaa ggtgttgtgt taaggagctc 60

ccctcattgc ctgagtgcct ggtctgggtt tcatacgtcg taggttcc 108

<210> 366

<211> 108

<212> DNA

<213> Artificial sequence

<400> 366

gactacatgg gacatagtga gctgatggcc ggcactgcca tggctgggag gtgtggaggc 60

tctgtggtca gcaccaatgg ccagcccgac cggtacgtcg taggttcc 108

<210> 367

<211> 108

<212> DNA

<213> Artificial sequence

<400> 367

gactacatgg gacatgggca caggcctgtg aggaccggcc atggagttat cagtgggagg 60

atcgaaattg gacagttact tcctgtctga ttctacgtcg taggttcc 108

<210> 368

<211> 108

<212> DNA

<213> Artificial sequence

<400> 368

gactacatgg gacatagaat ggcatgtgga tcagcctttg gagcttcgtg tagcgctgtt 60

tttggacaag tgctgtgtca tttctccact gtgtacgtcg taggttcc 108

<210> 369

<211> 108

<212> DNA

<213> Artificial sequence

<400> 369

gactacatgg gacatagaga acgctccatc ctgattatgt aaatcaaagt ccaactgtca 60

acattatgca ctagcaaaga attctcctct atgtacgtcg taggttcc 108

<210> 370

<211> 108

<212> DNA

<213> Artificial sequence

<400> 370

gactacatgg gacatagcca aggacaaagg gtcagatctg ccttgtacaa atagagggcc 60

aggcgagatg accctggaag actcgtacat agttacgtcg taggttcc 108

<210> 371

<211> 108

<212> DNA

<213> Artificial sequence

<400> 371

gactacatgg gacatgcctg tcgtctgctg ttgtcatgtg gagctcagca aacggtggga 60

gtcctagggg acaacataca cagcttagca gcatacgtcg taggttcc 108

<210> 372

<211> 108

<212> DNA

<213> Artificial sequence

<400> 372

gactacatgg gacatgcctg cggcagggca ggcagggact cagattccct ccatcccatc 60

gctgaggtct tacttccttc ttccgcccgc ctttacgtcg taggttcc 108

<210> 373

<211> 108

<212> DNA

<213> Artificial sequence

<400> 373

gactacatgg gacataaagc ccgggaggtg taccgttcct ttggccccca tccccctcac 60

caggggaccc tgccctactc actgtcttcc agttacgtcg taggttcc 108

<210> 374

<211> 108

<212> DNA

<213> Artificial sequence

<400> 374

gactacatgg gacatggatt ggggagggag gggatgagcc gagcaggccc cccagcgtgc 60

agggcacctg gatgccgacc acattggtcc gtttacgtcg taggttcc 108

<210> 375

<211> 108

<212> DNA

<213> Artificial sequence

<400> 375

gactacatgg gacatgttaa ccgcacactg gctcctgaag ccagggaaca ttcccttcaa 60

gatggcagag gaaaggcggg ggcagtgggg tcatacgtcg taggttcc 108

<210> 376

<211> 108

<212> DNA

<213> Artificial sequence

<400> 376

gactacatgg gacatgccct tcgcctgcag ctggcaggac tgcaacaaga agttcgcgcg 60

ctccgacgag ctggcgcggc actaccgcac acatacgtcg taggttcc 108

<210> 377

<211> 108

<212> DNA

<213> Artificial sequence

<400> 377

gactacatgg gacatctagg ccatgctaaa attcctgcca ttggcaccac tcctaaaaag 60

tctcctacgt ggaaagcagt aaagacatgc agctacgtcg taggttcc 108

<210> 378

<211> 108

<212> DNA

<213> Artificial sequence

<400> 378

gactacatgg gacatgagcc tgcagccagg ccaagaagcc gtgggcaagc cctacagtcg 60

cctagtgctc gcctacacag ctccctttga tcttacgtcg taggttcc 108

<210> 379

<211> 108

<212> DNA

<213> Artificial sequence

<400> 379

gactacatgg gacatctcct gcaaaaggtg tggtttgcct agctcagggt cttatctgcc 60

ctcgccatgt gaagaatctt ctggccccag acctacgtcg taggttcc 108

<210> 380

<211> 108

<212> DNA

<213> Artificial sequence

<400> 380

gactacatgg gacatcctgg accttcactg tcccagtccc cactgtcgct ctggtgactg 60

tgacatccgg atgggcggtg ctgaaggagc ggatacgtcg taggttcc 108

<210> 381

<211> 108

<212> DNA

<213> Artificial sequence

<400> 381

gactacatgg gacatgtgac attcttgtca cggccggctg tagcctccag tcgtctctct 60

tcctgtgact tgggttctgc ggctgcacca cgctacgtcg taggttcc 108

<210> 382

<211> 108

<212> DNA

<213> Artificial sequence

<400> 382

gactacatgg gacatcatac tagaacatcc aaggtgtttc tgtaggaaca agctattcct 60

aaacatgcac agatgtgaag tctttttgct gggtacgtcg taggttcc 108

<210> 383

<211> 108

<212> DNA

<213> Artificial sequence

<400> 383

gactacatgg gacataggca gttcccatcc cctgttgtgg ccagaggaag aagccgttta 60

cagctcgtgc acacagtgga ccaccagttc aggtacgtcg taggttcc 108

<210> 384

<211> 108

<212> DNA

<213> Artificial sequence

<400> 384

gactacatgg gacataccac actgtgacca ctacgctgag gagatgtgaa ccctggcctc 60

tgtctctccc acactctggc ccaggcccta tgctacgtcg taggttcc 108

<210> 385

<211> 108

<212> DNA

<213> Artificial sequence

<400> 385

gactacatgg gacatgagaa tcgaggtgct atgtgcacct gcaatcggtg ggacagacaa 60

tctcaaggct gctctcaaca gtgggcacct gtttacgtcg taggttcc 108

<210> 386

<211> 108

<212> DNA

<213> Artificial sequence

<400> 386

gactacatgg gacatttaac ccttcctatt actctaggag tgaatccatt tatctggttt 60

ctttcctcca ggatagaatg accaaagaag ggatacgtcg taggttcc 108

<210> 387

<211> 108

<212> DNA

<213> Artificial sequence

<400> 387

gactacatgg gacatggaca acctgtggct cccactcagg agcccagggg aggacttcca 60

gggcagggtg gggtgagaag ctcaacaaga tggtacgtcg taggttcc 108

<210> 388

<211> 108

<212> DNA

<213> Artificial sequence

<400> 388

gactacatgg gacatgtgtg cttaatccca cctcacccac atgcatgccc tgaggtcaat 60

gcacctttcc ataagcacac ctcacactct ccttacgtcg taggttcc 108

<210> 389

<211> 108

<212> DNA

<213> Artificial sequence

<400> 389

gactacatgg gacataagag tcaacaaccc agggggatcc acagaccacc caagtggatt 60

gtgacgttgt cctggccttc tcgtgggggt cagtacgtcg taggttcc 108

<210> 390

<211> 108

<212> DNA

<213> Artificial sequence

<400> 390

gactacatgg gacatagatt atactagctt ttctttcttt tttaaatgaa agaggaacag 60

caaaaaggtg tgtcccattt ccagacctgg ccttacgtcg taggttcc 108

<210> 391

<211> 108

<212> DNA

<213> Artificial sequence

<400> 391

gactacatgg gacatttaac agagaaaaca aaacagctcc ctgaagactg cagtgtgtgg 60

gcagggcaca agtccaacct ggggtggaat ttctacgtcg taggttcc 108

<210> 392

<211> 108

<212> DNA

<213> Artificial sequence

<400> 392

gactacatgg gacatttatt tcattcataa tgcatcttta tacattcatt gaacatcttc 60

cagacaatct tacaggctgg ttaggtaggc gaatacgtcg taggttcc 108

<210> 393

<211> 108

<212> DNA

<213> Artificial sequence

<400> 393

gactacatgg gacatcattc acccttcctc tgggttaagg gactttgggg cattccaaac 60

atacctcaag aggcatgagg ctggtggctg tactacgtcg taggttcc 108

<210> 394

<211> 108

<212> DNA

<213> Artificial sequence

<400> 394

gactacatgg gacatggcca gtaattaagg tatgctattt aacaatatca caaacgcact 60

ctgaattctg acaagattcg tgactctcca aattacgtcg taggttcc 108

<210> 395

<211> 108

<212> DNA

<213> Artificial sequence

<400> 395

gactacatgg gacatacctc ccttcccccc aggctgctct gcaaggaagg gcctgggtgg 60

ccctcgctgg gatgccaaat ctctaataca atatacgtcg taggttcc 108

<210> 396

<211> 108

<212> DNA

<213> Artificial sequence

<400> 396

gactacatgg gacataatgt gaatgcaacc acacaccgat cttctaataa accatacatg 60

ctaagcagtg ggctggctca cctgtatcca ctctacgtcg taggttcc 108

<210> 397

<211> 108

<212> DNA

<213> Artificial sequence

<400> 397

gactacatgg gacatgtagg agctgacctg tctaagctgt aggtggcagt ggacgaggag 60

taggaaattg cagttgtcat ggttaatgaa tgctacgtcg taggttcc 108

<210> 398

<211> 108

<212> DNA

<213> Artificial sequence

<400> 398

gactacatgg gacatgtttg actcgggtgg cctccaactt gggagggagt gactgctgta 60

ggcagctctg cctgctgatt catggtttca ggctacgtcg taggttcc 108

<210> 399

<211> 108

<212> DNA

<213> Artificial sequence

<400> 399

gactacatgg gacatccagg ggtgaagact gttcgtttcc cagatggcca gatttggaaa 60

ccagacattc ttctctataa caggtaagca tattacgtcg taggttcc 108

<210> 400

<211> 108

<212> DNA

<213> Artificial sequence

<400> 400

gactacatgg gacatatcct gcctgggggg ccaagcagtg gtttgctccc aagccctctt 60

tcagcctcac tgcttagggc aacatggtgt aagtacgtcg taggttcc 108

<210> 401

<211> 108

<212> DNA

<213> Artificial sequence

<400> 401

gactacatgg gacattcact tggctcgaag ggcaagctgc cggtcattgg ggcaaaccca 60

ctgatcattc ccgctgccga agatggtgtc ggctacgtcg taggttcc 108

<210> 402

<211> 108

<212> DNA

<213> Artificial sequence

<400> 402

gactacatgg gacatgaaat agagaacctt gcgaatcgga tcaacgaaag tctgcgttgg 60

gatggaattc ttgctgaccc ggaggcagag aagtacgtcg taggttcc 108

<210> 403

<211> 108

<212> DNA

<213> Artificial sequence

<400> 403

gactacatgg gacatacgat cttggtgtag ctggcgggtg ccttcctcac cacctgcgag 60

ctgatggagg ggagatcgag caggaccatc ttctacgtcg taggttcc 108

<210> 404

<211> 108

<212> DNA

<213> Artificial sequence

<400> 404

gactacatgg gacatacgct tcttcaaaat ctgccaggat ttgctgtcct aactcagtct 60

gtgcagcctt cactctgtga ggaagagaga acatacgtcg taggttcc 108

<210> 405

<211> 108

<212> DNA

<213> Artificial sequence

<400> 405

gactacatgg gacatacaca cgaagcataa cacccaagat gtgccaaaag ctgctgaaag 60

gaaggaaacc tgaaactcta cttacttgct cggtacgtcg taggttcc 108

<210> 406

<211> 108

<212> DNA

<213> Artificial sequence

<400> 406

gactacatgg gacatcaccc ttctgtacaa ggtgaatgga atcctcacgc tggccacctt 60

cctttcctgc cggatccttc tcttcccctt cattacgtcg taggttcc 108

<210> 407

<211> 108

<212> DNA

<213> Artificial sequence

<400> 407

gactacatgg gacatgggga gcaggtctgg agccagtatt cctctcggca cgcgtttgcc 60

tctagagtct ggatgaagat cctcagactg aggtacgtcg taggttcc 108

<210> 408

<211> 108

<212> DNA

<213> Artificial sequence

<400> 408

gactacatgg gacatgtcag gcctcacagg tgctgggcag gaatcacaga ggcttgctct 60

gcatcatgtc ttccgttaac ctgaagcttt gggtacgtcg taggttcc 108

<210> 409

<211> 108

<212> DNA

<213> Artificial sequence

<400> 409

gactacatgg gacatgaaac cggagccagt caagattggc tgcctcatgt tgaggttcag 60

agttacgact cggactggac agaggcgccg gcatacgtcg taggttcc 108

<210> 410

<211> 108

<212> DNA

<213> Artificial sequence

<400> 410

gactacatgg gacatgggct gggtaataag aggaccatat gcacgggctg aggttttacc 60

ttctgggtca tctcggatca ccagcagccc gtttacgtcg taggttcc 108

<210> 411

<211> 108

<212> DNA

<213> Artificial sequence

<400> 411

gactacatgg gacatatcag actggaagaa cagtgtcatc agtggctgca tcacgggagg 60

agctattggt ttcagaggtt agtaaacggc tcttacgtcg taggttcc 108

<210> 412

<211> 108

<212> DNA

<213> Artificial sequence

<400> 412

gactacatgg gacatatggg ctccttctcg gcaaccctaa gaaggagaag atggggagga 60

aagaagccct tcctcagcag ccactcggag ctctacgtcg taggttcc 108

<210> 413

<211> 108

<212> DNA

<213> Artificial sequence

<400> 413

gactacatgg gacatcctgg gcagagcagt gcagaatcca caatggccca cccaacctcg 60

tcctggccag ggcacttact ggctgattgc ttatacgtcg taggttcc 108

<210> 414

<211> 108

<212> DNA

<213> Artificial sequence

<400> 414

gactacatgg gacattcttg gtcccacccc cacccatcct gacctttcca ttccgattgt 60

ggatcctgaa cgggattgtg ggggagttga gcatacgtcg taggttcc 108

<210> 415

<211> 108

<212> DNA

<213> Artificial sequence

<400> 415

gactacatgg gacataccga gctggctggc ctgcagggag gagtcaggga acagagggag 60

aggagggtgg gaggggagag gattaatgaa tggtacgtcg taggttcc 108

<210> 416

<211> 108

<212> DNA

<213> Artificial sequence

<400> 416

gactacatgg gacatcttgc tcccagactc ctagaaagcc aagaggccgg gcgccgcctt 60

ctaagaagaa aacgagtccg cagagggggc tggtacgtcg taggttcc 108

<210> 417

<211> 108

<212> DNA

<213> Artificial sequence

<400> 417

gactacatgg gacattgttc ccatctgtca gcaaaactgt tgaactatac tcagctgaag 60

tcctcatcgg gattctgttg atccagcaga cggtacgtcg taggttcc 108

<210> 418

<211> 108

<212> DNA

<213> Artificial sequence

<400> 418

gactacatgg gacatgccag gtgcccgttc ttggccttgg tgatgagcag ctccgagccg 60

ggtgtgaagt caatggtgcc atccctgccg gggtacgtcg taggttcc 108

<210> 419

<211> 108

<212> DNA

<213> Artificial sequence

<400> 419

gactacatgg gacatcatgc acccctgcgc ccggtcccgc cacctcctca ccgtctctgg 60

ccgcttcttg tcactgagct cgctccggtc aaatacgtcg taggttcc 108

<210> 420

<211> 108

<212> DNA

<213> Artificial sequence

<400> 420

gactacatgg gacatttggc atttccaaag gcctaggagt ggacaggggt atgagggaca 60

cctggcactc tccccctgcc ctccccaccc cgctacgtcg taggttcc 108

<210> 421

<211> 108

<212> DNA

<213> Artificial sequence

<400> 421

gactacatgg gacatatagt cgttggagtt cctatcaaac ttgtaggtgg gcgggaagag 60

taggcggccc tcctggaact cccggagcag cggtacgtcg taggttcc 108

<210> 422

<211> 108

<212> DNA

<213> Artificial sequence

<400> 422

gactacatgg gacatagggg aaagcacatc catgaggaaa ctgctccacg agtcattaaa 60

ggcagcatcg gaaaggaggc ttatgatccc agatacgtcg taggttcc 108

<210> 423

<211> 108

<212> DNA

<213> Artificial sequence

<400> 423

gactacatgg gacattatac ggcttccacg tgtttccacg cctcaggctc cagcttatgc 60

acctgtggga acaagtgggg aaccactatg cagtacgtcg taggttcc 108

<210> 424

<211> 108

<212> DNA

<213> Artificial sequence

<400> 424

gactacatgg gacatcacct ctacttacga gtagtccatg tcctccggcc ccgggaaggg 60

ctcaaggggc cagttaaaga agcagttgag gaatacgtcg taggttcc 108

<210> 425

<211> 108

<212> DNA

<213> Artificial sequence

<400> 425

gactacatgg gacatcacca aagccattca gagccagggc gatgaagatg agcacggaga 60

gagctgcagg gacatggaaa gctcgtgagt ggatacgtcg taggttcc 108

<210> 426

<211> 108

<212> DNA

<213> Artificial sequence

<400> 426

gactacatgg gacatagcca gaggcaaggc cagggctgcc ctttttccga ttcagggcct 60

ggcgcggagg cttagggatg gccctcttga ccttacgtcg taggttcc 108

<210> 427

<211> 108

<212> DNA

<213> Artificial sequence

<400> 427

gactacatgg gacatagccc aggcccacct gcatgtgatg ataatggtct tctcgccatc 60

ccaagatggg ttgtcagcca tgatcttggc atgtacgtcg taggttcc 108

<210> 428

<211> 108

<212> DNA

<213> Artificial sequence

<400> 428

gactacatgg gacatagatg aaatagtctt gagccagtcg tcatagaggt tgaagagaac 60

catctggggc tcagtggctt taaggatgag atctacgtcg taggttcc 108

<210> 429

<211> 108

<212> DNA

<213> Artificial sequence

<400> 429

gactacatgg gacatcatca ccaggccata atactgcacg atgaatgagg caaactgcag 60

gcctctgatg atcccatatg aattcgtatg gtttacgtcg taggttcc 108

<210> 430

<211> 108

<212> DNA

<213> Artificial sequence

<400> 430

gactacatgg gacatccatc gctctaataa aggggtaatg ccacgcatga aaaagagcca 60

gactcgccaa ccggcagccc agaagccaca gcctacgtcg taggttcc 108

<210> 431

<211> 108

<212> DNA

<213> Artificial sequence

<400> 431

gactacatgg gacatgattg ttgtacaagt aaggaaaagc aatcttgtac tcagtgcgga 60

taggctgccg gatgataatc ttgttaatat cattacgtcg taggttcc 108

<210> 432

<211> 108

<212> DNA

<213> Artificial sequence

<400> 432

gactacatgg gacatgggaa cctcgggctg gacagggctc ccctcctccc ttcctgtctg 60

gcttctgccc tcagagtctc accaccaaat gattacgtcg taggttcc 108

<210> 433

<211> 108

<212> DNA

<213> Artificial sequence

<400> 433

gactacatgg gacatgtgcc tactccacag ggacatgcag gcgctgggtg tcctattggc 60

agagatggtg tttgccacca gggtgcggac gcttacgtcg taggttcc 108

<210> 434

<211> 108

<212> DNA

<213> Artificial sequence

<400> 434

gactacatgg gacatctaag cagcgaggac ttcttcctga gcggcagcaa ggatcgtacc 60

gtgcgcctct ggccgctgta caactacggc gactacgtcg taggttcc 108

<210> 435

<211> 108

<212> DNA

<213> Artificial sequence

<400> 435

gactacatgg gacattgcag gatttcccat caaagaagat ttcctggaac aatccgaaca 60

gctatttggg gcaaagcccg tgagcctgac gggtacgtcg taggttcc 108

<210> 436

<211> 108

<212> DNA

<213> Artificial sequence

<400> 436

gactacatgg gacatcgcta tggcttggat tcagatctca gctgcaaggt ctgtagggat 60

aggggcaggg tggggggtgg atggagggag aggtacgtcg taggttcc 108

<210> 437

<211> 108

<212> DNA

<213> Artificial sequence

<400> 437

gactacatgg gacataaaaa gatctgggcc aatttgaaga gctcatggcc catttcaaca 60

ctggacggcc cgtggcgaac ctccaccacg tagtacgtcg taggttcc 108

<210> 438

<211> 108

<212> DNA

<213> Artificial sequence

<400> 438

gactacatgg gacataagag gagcgtccga gtcctttccc accaagtaac ctttagaaag 60

tctttttaga aacagctcat cctcaggtct gcatacgtcg taggttcc 108

<210> 439

<211> 108

<212> DNA

<213> Artificial sequence

<400> 439

gactacatgg gacattagct ctcagaggct taagactgca ggattttgga ggctaaagaa 60

atctctgtgg ttttcagctt tcatgttggc gactacgtcg taggttcc 108

<210> 440

<211> 108

<212> DNA

<213> Artificial sequence

<400> 440

gactacatgg gacatgacgg agcctctccg tgctgtcttc aagcactatc attgcgaatc 60

ctgacatccc agaggcctat aagcttcgtg gattacgtcg taggttcc 108

<210> 441

<211> 108

<212> DNA

<213> Artificial sequence

<400> 441

gactacatgg gacataaaag gggtttcact gctccacacc acactttcac tgctcacaaa 60

ctacttctcc ctttttgaag gacgagtctc gaatacgtcg taggttcc 108

<210> 442

<211> 108

<212> DNA

<213> Artificial sequence

<400> 442

gactacatgg gacatctgga gagatcctgg gctgcacatc cccccgactg tccaaagagg 60

tggaggccgt tgtgtaagtt aaaaatgggg gcctacgtcg taggttcc 108

<210> 443

<211> 108

<212> DNA

<213> Artificial sequence

<400> 443

gactacatgg gacatccgct gctgacaccc tatgaggtaa caccaagctc tgggagagag 60

tgggctttgg acgtggttct caaaggtgag gtctacgtcg taggttcc 108

<210> 444

<211> 108

<212> DNA

<213> Artificial sequence

<400> 444

gactacatgg gacattggtc aatgaagccg ttggtgtctg gtacgaggaa caaaggtctg 60

atttcgagct ccatctgcct catctgactg tggtacgtcg taggttcc 108

<210> 445

<211> 108

<212> DNA

<213> Artificial sequence

<400> 445

gactacatgg gacatagccc ctactctccc accacctggg ctcttttcta tctcagccct 60

gtctggttta ttcgcaactt cctccttcgc aactacgtcg taggttcc 108

<210> 446

<211> 108

<212> DNA

<213> Artificial sequence

<400> 446

gactacatgg gacataacca ggagcctgca gtgatagctg gacaagggac aattgccctg 60

gaagtgctga accaggtaaa atagctgagt tcttacgtcg taggttcc 108

<210> 447

<211> 108

<212> DNA

<213> Artificial sequence

<400> 447

gactacatgg gacataaaag acaagatgga caagaaccca gcttccccat tgctaacctg 60

cagtgtgctg agagaataaa ggtgaggctc ggatacgtcg taggttcc 108

<210> 448

<211> 108

<212> DNA

<213> Artificial sequence

<400> 448

gactacatgg gacattttgt gcatgaagac agcagccaca tcattgcttt atgtggtgag 60

tgagtgactg aaggatgatg ggaggtcggt ctatacgtcg taggttcc 108

<210> 449

<211> 108

<212> DNA

<213> Artificial sequence

<400> 449

gactacatgg gacatcttct cacgtgccag ccgctccatt tcatgctcat attccttctc 60

cttcctcttc agggactggc acacagagta aggtacgtcg taggttcc 108

<210> 450

<211> 108

<212> DNA

<213> Artificial sequence

<400> 450

gactacatgg gacattcgtg agtttactcc ctgaaaaaca acaaaaaacc ctggtgaaaa 60

atgtgtacac cccaagaacc aagtttgaat gtatacgtcg taggttcc 108

<210> 451

<211> 108

<212> DNA

<213> Artificial sequence

<400> 451

gactacatgg gacatagctc tggagacagg tggggcacag cttccgtgac ttttctttgg 60

tgggaaaaag gtatactttg tagggttaaa ctgtacgtcg taggttcc 108

<210> 452

<211> 108

<212> DNA

<213> Artificial sequence

<400> 452

gactacatgg gacatcgttc tgggctgatt cagagccgcg tttccctccc tcagcgattc 60

ctgccaggag agcagacagc tggtaccgcc ccatacgtcg taggttcc 108

<210> 453

<211> 108

<212> DNA

<213> Artificial sequence

<400> 453

gactacatgg gacattcctt ctggttaata cttgcaaaaa tctcaccatc ttctatctgc 60

agaaagaaaa aaaaaaaaat caggaaagca gtttacgtcg taggttcc 108

<210> 454

<211> 108

<212> DNA

<213> Artificial sequence

<400> 454

gactacatgg gacatacctg gcagagatca gcatgtattg aggtcagctg gtttgtattc 60

atctgcatct tgtctatggc ttgcttaagg atgtacgtcg taggttcc 108

<210> 455

<211> 108

<212> DNA

<213> Artificial sequence

<400> 455

gactacatgg gacataggtg gtgaaggcct cggggtggtg aagtcatggg gctgcaggct 60

caacctcctt tctcttgttg acagcactga cgttacgtcg taggttcc 108

<210> 456

<211> 108

<212> DNA

<213> Artificial sequence

<400> 456

gactacatgg gacatgcgcg cggaacatct ggtccaggct gctgttcttc ttggccgaga 60

tctcgaagta ggcgcagcgc tgggggtcgt cgctacgtcg taggttcc 108

<210> 457

<211> 108

<212> DNA

<213> Artificial sequence

<400> 457

gactacatgg gacataccag tgcgagcgca ggaagttcag gagcaggatg gtgacgctta 60

gagagtagca ggccaggtag ggggatccga aggtacgtcg taggttcc 108

<210> 458

<211> 108

<212> DNA

<213> Artificial sequence

<400> 458

gactacatgg gacatcttgc agctggacaa gggcggcaat gccaaggact tcagcccagg 60

gctgtttgaa gacccttccg tggccttcgc tactacgtcg taggttcc 108

<210> 459

<211> 108

<212> DNA

<213> Artificial sequence

<400> 459

gactacatgg gacatctgct ggctggtgtg gtagccaggc tgtcctgcag gtacccactg 60

gccttctcac agatggtcag gctggctgga ccatacgtcg taggttcc 108

<210> 460

<211> 108

<212> DNA

<213> Artificial sequence

<400> 460

gactacatgg gacatggccg ttggccctac ccctccccgc gccgacttca cctgtcaagg 60

cgcggcggat ttttgaagcc gttgagcgct gcgtacgtcg taggttcc 108

<210> 461

<211> 108

<212> DNA

<213> Artificial sequence

<400> 461

gactacatgg gacatactct gttttcgatt gtctagtgca gaagcaagtc ctccgacagc 60

ctgaggatcc tcataggtta ccctggagat gaatacgtcg taggttcc 108

<210> 462

<211> 108

<212> DNA

<213> Artificial sequence

<400> 462

gactacatgg gacattggct tgcctatggc cccgaagtat gcaagtacct gttctgaatt 60

ggctgtgatg gggccagtca cactcagagc tggtacgtcg taggttcc 108

<210> 463

<211> 108

<212> DNA

<213> Artificial sequence

<400> 463

gactacatgg gacattcggg gaccagggcc cccaggagga ggccgggcag ctggccaagc 60

aggagggcat cctcttcaag gatgtcctgt ccctacgtcg taggttcc 108

<210> 464

<211> 108

<212> DNA

<213> Artificial sequence

<400> 464

gactacatgg gacattctgc ctgcagactg cgtttgtgga acacctgaat ggctccttcc 60

tgtttgacag catgtacgct gaggactcag aggtacgtcg taggttcc 108

<210> 465

<211> 108

<212> DNA

<213> Artificial sequence

<400> 465

gactacatgg gacatgggaa ctcttacctt tgtgggtact tgaagattaa aggccttact 60

gaggtaagca cttgctcaca caaaccagca ctatacgtcg taggttcc 108

<210> 466

<211> 108

<212> DNA

<213> Artificial sequence

<400> 466

gactacatgg gacatgaccc agtgctcgga aaagctggtg cagctcatcc tgcacgaata 60

cagtatcctt ccctgaaaga cacgtgaagg aggtacgtcg taggttcc 108

<210> 467

<211> 108

<212> DNA

<213> Artificial sequence

<400> 467

gactacatgg gacatcagcc cggcctggcc accctacggc gaccactact acgggtaccc 60

gcccgaggat ccctacgact actaccaccc cgatacgtcg taggttcc 108

<210> 468

<211> 108

<212> DNA

<213> Artificial sequence

<400> 468

gactacatgg gacatgccgc catgaaccag aaacgggagg tcatgcagca ggtgagtcta 60

cctgtctccc caggacccta ggctgaacac ccttacgtcg taggttcc 108

<210> 469

<211> 108

<212> DNA

<213> Artificial sequence

<400> 469

gactacatgg gacatccccc taccaggtac tgctgtctag tggccctcaa gcatgacctg 60

ccggctaatg gggacatgtg tgtgtcagtg ctgtacgtcg taggttcc 108

<210> 470

<211> 108

<212> DNA

<213> Artificial sequence

<400> 470

gactacatgg gacattgccc tcgcgatcgc tggagccccc tgaggaactc acgcagacgc 60

ggctgcaccg cctcatcaat cccaacttct acgtacgtcg taggttcc 108

<210> 471

<211> 108

<212> DNA

<213> Artificial sequence

<400> 471

gactacatgg gacatccgtg cttacatcct ggatgtggcc tcagagatgg agcaggtgga 60

cggcggctac atgctctggt tccggcgtgt gcttacgtcg taggttcc 108

<210> 472

<211> 108

<212> DNA

<213> Artificial sequence

<400> 472

gactacatgg gacatactct tcatggtcac atgttctgtg tttcttttca gtggatatgc 60

tgctgatgaa atcactcact gcatacggcc tcatacgtcg taggttcc 108

<210> 473

<211> 108

<212> DNA

<213> Artificial sequence

<400> 473

gactacatgg gacatcactc ccttcccctt gttccctgca gtgctccgct cagccttacc 60

cgagtcacag tggtcctgct ggtggctgcc agctacgtcg taggttcc 108

<210> 474

<211> 108

<212> DNA

<213> Artificial sequence

<400> 474

gactacatgg gacatgccag ggccttgcag aaggcgctcc aggcgtggaa gcagcgggta 60

aaggcgtgct gctcattgcc cttgcggacc aggtacgtcg taggttcc 108

<210> 475

<211> 108

<212> DNA

<213> Artificial sequence

<400> 475

gactacatgg gacatctggt tctgcagcga gaagtcgatg ttgtaccact cagcggcacg 60

gtctgcgggc ttgggcggca tgaccaggtt gggtacgtcg taggttcc 108

<210> 476

<211> 108

<212> DNA

<213> Artificial sequence

<400> 476

gactacatgg gacatctgaa aatctcaggt ccaggatctc cctcagggtg tcgtcgcatc 60

cgccgatgca gcaaacaaac tccagaggca tggtacgtcg taggttcc 108

<210> 477

<211> 108

<212> DNA

<213> Artificial sequence

<400> 477

gactacatgg gacatcctgt ctggaacata ccacagtgtc caaggcttga ggccgccact 60

tgctcttggg cttagatctg acctctacca cagtacgtcg taggttcc 108

<210> 478

<211> 108

<212> DNA

<213> Artificial sequence

<400> 478

gactacatgg gacatcaacg acaaaggctt ttactcatct caggcaattg agaaagccaa 60

tgaactggcc agtgtggaga agtccatcat tgatacgtcg taggttcc 108

<210> 479

<211> 108

<212> DNA

<213> Artificial sequence

<400> 479

gactacatgg gacatctgcc tacccacgac aaggagacag aggagctggt agccagccgc 60

cagatgagct tcctaaagct gaccctgggt ctgtacgtcg taggttcc 108

<210> 480

<211> 108

<212> DNA

<213> Artificial sequence

<400> 480

gactacatgg gacatgattt ttttgtgtat tttgaaaaat ttgaactata tgcctttccc 60

tctcttcttt ttccttttcc tgccagaaac aagtacgtcg taggttcc 108

<210> 481

<211> 108

<212> DNA

<213> Artificial sequence

<400> 481

gactacatgg gacataatgt ggccgtgggc atatgggtaa ggggacctgt ggtggtgttg 60

gccaagtggg ctctcagggt tggtgcttgg ggttacgtcg taggttcc 108

<210> 482

<211> 108

<212> DNA

<213> Artificial sequence

<400> 482

gactacatgg gacatggcca gctacctcaa gatgctcccc atgggcctga tcatcatgcc 60

gggcatgatc agccgcgcat tgttcccagg tagtacgtcg taggttcc 108

<210> 483

<211> 108

<212> DNA

<213> Artificial sequence

<400> 483

gactacatgg gacatcaggg gaggtgggag agagctgaag agcctcccac gacgaccgct 60

gcctgccttc cactcgcctg caggctggtc atatacgtcg taggttcc 108

<210> 484

<211> 108

<212> DNA

<213> Artificial sequence

<400> 484

gactacatgg gacatgcccg gaaaacacct gagtccttcc tgggccccaa cgcggccctg 60

gtgaacctgg actcactggt gaccaggcct gcctacgtcg taggttcc 108

<210> 485

<211> 108

<212> DNA

<213> Artificial sequence

<400> 485

gactacatgg gacattcacc ttgtaaactt ctgcagttta gacgtagatt ctgggacaaa 60

catggggatg gtccttttgt gcctgaaaca aattacgtcg taggttcc 108

<210> 486

<211> 108

<212> DNA

<213> Artificial sequence

<400> 486

gactacatgg gacatggctg agtcgactct gcagtttctc caaggtatcc tctggtatgt 60

cccttttccc tgctcctcag gccagcaggt ggctacgtcg taggttcc 108

<210> 487

<211> 108

<212> DNA

<213> Artificial sequence

<400> 487

gactacatgg gacatgccga tattgagtcc ctgcagcgtg agatccagat ggactcccca 60

atgctgctgg ctgacctgcc tgacctccag gactacgtcg taggttcc 108

<210> 488

<211> 108

<212> DNA

<213> Artificial sequence

<400> 488

gactacatgg gacatcgcta agagccgctg ccaggggtac tggaacgagg ggcgcgccgt 60

ggccaggggg gacagacgcc tactcacggg gcatacgtcg taggttcc 108

<210> 489

<211> 108

<212> DNA

<213> Artificial sequence

<400> 489

gactacatgg gacatccttt ttattttagg tccctgcagg cttcagtgtg gctgccagtg 60

tccgggtagg aaacgctctg ggtgctggag acatacgtcg taggttcc 108

<210> 490

<211> 108

<212> DNA

<213> Artificial sequence

<400> 490

gactacatgg gacatcttcc aatagtgcct gtgaaaaatg tagatgaggc cataaatttc 60

ataaatgaac gtgaaaagcc tctggctctt tattacgtcg taggttcc 108

<210> 491

<211> 108

<212> DNA

<213> Artificial sequence

<400> 491

gactacatgg gacatgtccc agggagtcag gctttggatc aaagtggtac ttacacagat 60

ggcctcaaac acgtgaaaga cactataaac cggtacgtcg taggttcc 108

<210> 492

<211> 108

<212> DNA

<213> Artificial sequence

<400> 492

gactacatgg gacataccga tgccagctcc accttgccca ggtgcccgca gaacacagtg 60

ctcacgatgt agatcataaa agtcagcacc tggtacgtcg taggttcc 108

<210> 493

<211> 108

<212> DNA

<213> Artificial sequence

<400> 493

gactacatgg gacattcagg ggccacgcag gtctggccac tgttcatgaa tttcccccag 60

gcgatgcgtc tgtgagaatc ccagactgga ctatacgtcg taggttcc 108

<210> 494

<211> 108

<212> DNA

<213> Artificial sequence

<400> 494

gactacatgg gacatcactc atggcacaaa gagagccccc ggagttgctg ggccccacta 60

caaggaaaca gaggatacat ggcagtgtcc agatacgtcg taggttcc 108

<210> 495

<211> 108

<212> DNA

<213> Artificial sequence

<400> 495

gactacatgg gacatctttg gaaacttgta gatagttggg aggacccaat ggtggggaag 60

ataagttttc ttcctgaata tgctgcatat ctgtacgtcg taggttcc 108

<210> 496

<211> 108

<212> DNA

<213> Artificial sequence

<400> 496

gactacatgg gacatcactg acacacatac acagttacct tgcaaataat ctgcgaggtc 60

tccaccattg caatactgac atagaaaaga gaatacgtcg taggttcc 108

<210> 497

<211> 108

<212> DNA

<213> Artificial sequence

<400> 497

gactacatgg gacatctacc aataaaactt cttgtagttt gatacgtttg aagttctggg 60

ttaccttctc catggttgtc caggcctgac gtatacgtcg taggttcc 108

<210> 498

<211> 108

<212> DNA

<213> Artificial sequence

<400> 498

gactacatgg gacattccct ccaaccccag gaaatcagtg tccagtccaa cttcttccaa 60

cactcccact cctacgaaac acctgaggac ccctacgtcg taggttcc 108

<210> 499

<211> 108

<212> DNA

<213> Artificial sequence

<400> 499

gactacatgg gacatgccag agtcctctga ggtcgatgct gctggtcggt ggcctggtgt 60

ctgtgttagc agaacatctc caacaccccc agatacgtcg taggttcc 108

<210> 500

<211> 108

<212> DNA

<213> Artificial sequence

<400> 500

gactacatgg gacattttaa cttagaagag gaatctcttg ttggcatttg aagcggctga 60

aagtgtaggc atcaaaccca gcctggtacg tattacgtcg taggttcc 108

<210> 501

<211> 108

<212> DNA

<213> Artificial sequence

<400> 501

gactacatgg gacatcaaca gcccttaagc agagaggact ctggaattca ggtagacagg 60

acaccgttag aagaacaaga tcaaaacaga aaatacgtcg taggttcc 108

<210> 502

<211> 108

<212> DNA

<213> Artificial sequence

<400> 502

gactacatgg gacatgcacc tcgattgtct cagctcaagg ttctgcacaa agtgtttagt 60

actggagtag cagaagttcc acctgatact cgatacgtcg taggttcc 108

<210> 503

<211> 108

<212> DNA

<213> Artificial sequence

<400> 503

gactacatgg gacataagtt tatacactct ctttctggaa tctgtacagt cccgtcttcg 60

acatggagaa gattccactc cacaggttct tactacgtcg taggttcc 108

<210> 504

<211> 108

<212> DNA

<213> Artificial sequence

<400> 504

gactacatgg gacatagaag gccttataca tgaacaagac acagttgctc agttcggacc 60

acaaaaagaa ccagtaagac agcagattca tcgtacgtcg taggttcc 108

<210> 505

<211> 108

<212> DNA

<213> Artificial sequence

<400> 505

gactacatgg gacatctttg atttgccctg tgttccattg cagaacgaga tgctggagga 60

gggccaagaa tatgctgtca tgctgtacac ctgtacgtcg taggttcc 108

<210> 506

<211> 108

<212> DNA

<213> Artificial sequence

<400> 506

gactacatgg gacatacatc tataagttgg atgccaagaa aagaataaac ttatccaaaa 60

tcgacaagta cttcaaggtg agttacatgt ttttacgtcg taggttcc 108

<210> 507

<211> 108

<212> DNA

<213> Artificial sequence

<400> 507

gactacatgg gacatccacg ccatccggca caccgtctat gccgcactgc aggacttctc 60

ccaggtgacc cttagggagc cgctgcggca ggctacgtcg taggttcc 108

<210> 508

<211> 108

<212> DNA

<213> Artificial sequence

<400> 508

gactacatgg gacatgtacg tgctctactc cctggacctg tacaatgaca gcgcccacta 60

cgcgctcacc aggttcaaca agcagttcct gtatacgtcg taggttcc 108

<210> 509

<211> 108

<212> DNA

<213> Artificial sequence

<400> 509

gactacatgg gacatacggc ttcgacgcca tgttccggga ggccaaccac aacgtgtcag 60

cgccctacgg gaggatcacc ctgcacgtct tcttacgtcg taggttcc 108

<210> 510

<211> 108

<212> DNA

<213> Artificial sequence

<400> 510

gactacatgg gacatgacat tttgaaacat gaagaataaa tgttgaagtg ttaatagtaa 60

aacatcctca ctttgatggt tgtagccttt gaatacgtcg taggttcc 108

<210> 511

<211> 108

<212> DNA

<213> Artificial sequence

<400> 511

gactacatgg gacataggta gacactgagg tcacttcaaa gtcacataag gcataaccta 60

cctgctctca tagagccttt cctggcaagt cgatacgtcg taggttcc 108

<210> 512

<211> 108

<212> DNA

<213> Artificial sequence

<400> 512

gactacatgg gacatataat ataggcgtgg tttttggtat caatttcttt caattgataa 60

ccaaaggcac actccagctt attgttggca cggtacgtcg taggttcc 108

<210> 513

<211> 108

<212> DNA

<213> Artificial sequence

<400> 513

gactacatgg gacattcctg gacctggagg cttggccatc ggtgctcctg aaggctgagg 60

ctgggtcatc atggctgctg gaggcggctg gactacgtcg taggttcc 108

<210> 514

<211> 108

<212> DNA

<213> Artificial sequence

<400> 514

gactacatgg gacatatctt ggaggataaa acagagacca tgacaaacag cagcatcacc 60

cagcctgccc cttctttctc ccaacctgtg cagtacgtcg taggttcc 108

<210> 515

<211> 108

<212> DNA

<213> Artificial sequence

<400> 515

gactacatgg gacatctgag gacaaaagag agtgaaaagg ggcaaagaac tttctttcct 60

gtctgtcatt ttgcagatgt aagtgggatc tgttacgtcg taggttcc 108

<210> 516

<211> 108

<212> DNA

<213> Artificial sequence

<400> 516

gactacatgg gacatgtcca gcaggcactc tggccttgtt atactgtgct tgaatatgaa 60

aaaaaaaaaa atcaaaatag tgctgacatt ctttacgtcg taggttcc 108

<210> 517

<211> 108

<212> DNA

<213> Artificial sequence

<400> 517

gactacatgg gacattgttg tatcccttct gaatgtaagt gtccactttc caaagtcctg 60

attttcctca tttttgggca tgaataatgt gcatacgtcg taggttcc 108

<210> 518

<211> 108

<212> DNA

<213> Artificial sequence

<400> 518

gactacatgg gacattcaac ttgaagaagc agttactagt gtcctgggtg ggccccaggg 60

aaaatagtac caactgcctg ttatatttaa ctgtacgtcg taggttcc 108

<210> 519

<211> 108

<212> DNA

<213> Artificial sequence

<400> 519

gactacatgg gacatacgtg ctcagcctcc cgtgcgctaa agctcaggtc cttcctgtca 60

tcctggtatc ctgcactgag gtgtggtgag tcctacgtcg taggttcc 108

<210> 520

<211> 108

<212> DNA

<213> Artificial sequence

<400> 520

gactacatgg gacatgtagc ttcctttacc gacgtgtata ttctatctag aacattgagc 60

tatggaagac tcccacctaa gggaattagt ttttacgtcg taggttcc 108

<210> 521

<211> 108

<212> DNA

<213> Artificial sequence

<400> 521

gactacatgg gacatctctt tgggagcact gcatctcctg ggggacttcc tggtcaactg 60

acgtgccagc tgaagttgtg tggggaaaac ccttacgtcg taggttcc 108

<210> 522

<211> 108

<212> DNA

<213> Artificial sequence

<400> 522

gactacatgg gacatcagca ccatgttggc aagtcgggag tagcaccaat gcccgtgaag 60

aatcaagagc ctctccaggt atcggaattt cggtacgtcg taggttcc 108

<210> 523

<211> 108

<212> DNA

<213> Artificial sequence

<400> 523

gactacatgg gacatactgg aagaggagct cctcgctgtt ttccagggag gattcggctt 60

ctaggtggct ttgcaaccag caggcatact ctttacgtcg taggttcc 108

<210> 524

<211> 108

<212> DNA

<213> Artificial sequence

<400> 524

gactacatgg gacataacag agtatgatga ttttaagttc tcttcataaa agtaaagcaa 60

ttattgtacg atacttagta ttgttacctg cagtacgtcg taggttcc 108

<210> 525

<211> 108

<212> DNA

<213> Artificial sequence

<400> 525

gactacatgg gacatgcgaa acctactcag gtcgttgttt ggcttctcgc attcgatcac 60

gctggtgaac gtcaaaggat tgaattcgga gactacgtcg taggttcc 108

<210> 526

<211> 108

<212> DNA

<213> Artificial sequence

<400> 526

gactacatgg gacatccagt gccaggccgg gctggaaggc gttcaccgcc ggcacgaagt 60

tgagcagcgc gatgaagaca aagtacacgt tggtacgtcg taggttcc 108

<210> 527

<211> 108

<212> DNA

<213> Artificial sequence

<400> 527

gactacatgg gacataggag aaccatgggt ttaatctcta aatcaccacc tccacgttgt 60

ttttagaaaa acaatcagat tttggtgaaa ctgtacgtcg taggttcc 108

<210> 528

<211> 108

<212> DNA

<213> Artificial sequence

<400> 528

gactacatgg gacatcacga cagaatcgtt atcagtatag agggcatata agtctgaaga 60

atgaagtatc caatgttcct cttcaaccga aagtacgtcg taggttcc 108

<210> 529

<211> 108

<212> DNA

<213> Artificial sequence

<400> 529

gactacatgg gacataatga acagctccac actctcattt tgcaacctag gaagtggggg 60

tgaggaatat gctcaggacg tcccctgaag acctacgtcg taggttcc 108

<210> 530

<211> 108

<212> DNA

<213> Artificial sequence

<400> 530

gactacatgg gacatgatgc gcacgcttgc cctctgaaat ttggcagctg taagttattt 60

tcacaagtaa gagccttgga catatacttt gggtacgtcg taggttcc 108

<210> 531

<211> 108

<212> DNA

<213> Artificial sequence

<400> 531

gactacatgg gacatcaccc aggcggccgg agccttccct gctgactcag ccctgccttg 60

gaagcctacg ctttagtggg ctgaattaaa ttctacgtcg taggttcc 108

<210> 532

<211> 108

<212> DNA

<213> Artificial sequence

<400> 532

gactacatgg gacatataaa acattttatt ctttttaagg ctctgaagag ctatacttgt 60

cagaatgatt tctgcaccag ggaatcattt agatacgtcg taggttcc 108

<210> 533

<211> 108

<212> DNA

<213> Artificial sequence

<400> 533

gactacatgg gacatagaat gcacaacccc aagagaggag ctctgtgccc atgagtgtga 60

ttaagacctt aagaagtctt ggagtggatt ctttacgtcg taggttcc 108

<210> 534

<211> 108

<212> DNA

<213> Artificial sequence

<400> 534

gactacatgg gacatcagtt ctatatgttc agtgccatat gctcaacata ataatataac 60

tgtagttaaa ctaatgataa ctaagtcaca atatacgtcg taggttcc 108

<210> 535

<211> 108

<212> DNA

<213> Artificial sequence

<400> 535

gactacatgg gacatgctga gtgccagctg cagctgcaca acttccccat ggacgaacac 60

tcctgcccgc tgattttctc cagctgtgag tactacgtcg taggttcc 108

<210> 536

<211> 108

<212> DNA

<213> Artificial sequence

<400> 536

gactacatgg gacatcttcg agcaatagat ggatgtctat taattccatc ccaccaccac 60

atgagccaca aggagataac acatcccaac agttacgtcg taggttcc 108

<210> 537

<211> 108

<212> DNA

<213> Artificial sequence

<400> 537

gactacatgg gacatgttga caaaatagca tgaggcacag gtaatcatgt gcctaaatgg 60

accagtaaga cagcacagcc cattgtgata atctacgtcg taggttcc 108

<210> 538

<211> 108

<212> DNA

<213> Artificial sequence

<400> 538

gactacatgg gacattccat gctgttctgc aatccctgca cacacgacgt ttgccgacgc 60

gccaatcagt gtcccgttac ctaaagtcaa aattacgtcg taggttcc 108

<210> 539

<211> 108

<212> DNA

<213> Artificial sequence

<400> 539

gactacatgg gacatcgtgc acccccaccc ccatgcagtc agcagcccct tacctcccag 60

gcaagcaccg aaggccaggg catacatgag cggtacgtcg taggttcc 108

<210> 540

<211> 108

<212> DNA

<213> Artificial sequence

<400> 540

gactacatgg gacatgatcc cctggtaaag gtgtacaagt gcctcaggta agcacaggga 60

aggctgcacc ctcactggga ttctgagagc tgatacgtcg taggttcc 108

<210> 541

<211> 108

<212> DNA

<213> Artificial sequence

<400> 541

gactacatgg gacattggcc acagggagat gcaccatacg cggaaacact acgtgcagcc 60

gacctggcct ttggggaggc gggcaggcgc agatacgtcg taggttcc 108

<210> 542

<211> 108

<212> DNA

<213> Artificial sequence

<400> 542

gactacatgg gacatggaaa gaaagctggg tacctttttt tggagttctt ggatattggt 60

ctcccaattt ttgtcctcct gtgagatctg tcttacgtcg taggttcc 108

<210> 543

<211> 108

<212> DNA

<213> Artificial sequence

<400> 543

gactacatgg gacatggcag ctcctccaat gtttgtgaag atcacttctg caatcaggac 60

ttgtcttgga tcaaggttga gcacctcaca caatacgtcg taggttcc 108

<210> 544

<211> 108

<212> DNA

<213> Artificial sequence

<400> 544

gactacatgg gacatgcctg ctccaagcca gccctgtgac ctcactctat gacatgttcc 60

tttcttctgg cttaaccttt gggctaactt tgttacgtcg taggttcc 108

<210> 545

<211> 108

<212> DNA

<213> Artificial sequence

<400> 545

gactacatgg gacatgagcc tgccccaaca cctcactcac tgagaactca cctggtcagt 60

acctcaaagg tcctgctcat cactgagtgc tcgtacgtcg taggttcc 108

<210> 546

<211> 108

<212> DNA

<213> Artificial sequence

<400> 546

gactacatgg gacatgcgcc tgcaagagaa aaagtagggc cttagtggca agggcagcag 60

tcctgttgcc cagactcaga cacttttctg cagtacgtcg taggttcc 108

<210> 547

<211> 108

<212> DNA

<213> Artificial sequence

<400> 547

gactacatgg gacatcctgc cgccccaggg agttacctca ctgaggtccg cgatggtgag 60

gctcatccca gccagctgct tccagacagg ctctacgtcg taggttcc 108

<210> 548

<211> 108

<212> DNA

<213> Artificial sequence

<400> 548

gactacatgg gacatccgag tccagtttcg tcgagcgagc cttccaggtc gaacatgggg 60

atgcaggggc agaagagctc ggagaggtgg tgctacgtcg taggttcc 108

<210> 549

<211> 108

<212> DNA

<213> Artificial sequence

<400> 549

gactacatgg gacattagca aatgagacta aaaaaagtac ccttacattc tgctgaagtg 60

tccaagtttt ccatcgtcac cttcgcccca cgatacgtcg taggttcc 108

<210> 550

<211> 108

<212> DNA

<213> Artificial sequence

<400> 550

gactacatgg gacatagcta agaaaggaac tgcagccaaa tcttcctctt ctgcacggat 60

ccgtcccagc aggatgagac catggatttt acatacgtcg taggttcc 108

<210> 551

<211> 108

<212> DNA

<213> Artificial sequence

<400> 551

gactacatgg gacatacctt tgcttcagct cataacatgg caccccgaaa gccccatgca 60

ggaatcatga gttacaatgc catcagtggc ctttacgtcg taggttcc 108

<210> 552

<211> 108

<212> DNA

<213> Artificial sequence

<400> 552

gactacatgg gacatatccc taagtgtctt tgccttaaaa atacagtttt accctttata 60

agtatcaata ttgcattact tttcctggga ccctacgtcg taggttcc 108

<210> 553

<211> 108

<212> DNA

<213> Artificial sequence

<400> 553

gactacatgg gacatcaggg ccagaggacg caagaggaac ctagcagtgg agtctcctgt 60

tttccttgat agcactacct gctggacact gggtacgtcg taggttcc 108

<210> 554

<211> 108

<212> DNA

<213> Artificial sequence

<400> 554

gactacatgg gacatagttc tttggatgac aatcgatatc catcattaca aaaataggta 60

actcgcgttc ctaccaaata gtctgttgtt agttacgtcg taggttcc 108

<210> 555

<211> 108

<212> DNA

<213> Artificial sequence

<400> 555

gactacatgg gacatttcaa agggaaaatg gttgaaaatt aatgattttg tatctgaatg 60

caaaaggagc taaaatagca tcacctccac tcctacgtcg taggttcc 108

<210> 556

<211> 108

<212> DNA

<213> Artificial sequence

<400> 556

gactacatgg gacattataa accatacgaa aaaagagttc tggtattctg gagcagtttt 60

gtgggcatat gtctgttgcc ctttgggaca ctgtacgtcg taggttcc 108

<210> 557

<211> 108

<212> DNA

<213> Artificial sequence

<400> 557

gactacatgg gacattctac aaccttgaaa tgatgcctta taattttctc actgacacct 60

gggtgcactg ttgagttttc agttacctaa ttgtacgtcg taggttcc 108

<210> 558

<211> 108

<212> DNA

<213> Artificial sequence

<400> 558

gactacatgg gacattggca gagatatgca ggcaatataa aaactgttgg aaggtggata 60

caggaagcct ttcagctgct gtggttttct atatacgtcg taggttcc 108

<210> 559

<211> 108

<212> DNA

<213> Artificial sequence

<400> 559

gactacatgg gacatttact tctatttatt actcttcgct tccaccatca aaaaaatatt 60

tttttttcct gtcagacaaa aaaaaaaaaa ccatacgtcg taggttcc 108

<210> 560

<211> 108

<212> DNA

<213> Artificial sequence

<400> 560

gactacatgg gacatgtctt cctgtgtcat cagtcaggca ctgatttcct aagtcaaatt 60

aaaccatcta ttcaaactat tatttgtaat ggctacgtcg taggttcc 108

<210> 561

<211> 108

<212> DNA

<213> Artificial sequence

<400> 561

gactacatgg gacatatatg gacagactct gactccttct cggaggataa ttgataccag 60

aaaaggtgct ctgaaagcaa gtctacttca tggtacgtcg taggttcc 108

<210> 562

<211> 108

<212> DNA

<213> Artificial sequence

<400> 562

gactacatgg gacatttcca aaactgggca gtgagttcaa caacaacgac aacaacagcc 60

gcagctcatc ctggccgtca tggagtttct tgatacgtcg taggttcc 108

<210> 563

<211> 108

<212> DNA

<213> Artificial sequence

<400> 563

gactacatgg gacatactac ttaaaagaaa catatgatat cctaagcaag aaggtaagta 60

ggtgagggag acaactaacc ccagataatt tgctacgtcg taggttcc 108

<210> 564

<211> 108

<212> DNA

<213> Artificial sequence

<400> 564

gactacatgg gacattacac acctctctgt gtaaacgaaa gttgcccgag gtgatctctt 60

gtcttcctgc ctaagaggca atgtcttagc tgttacgtcg taggttcc 108

<210> 565

<211> 108

<212> DNA

<213> Artificial sequence

<400> 565

gactacatgg gacatcacgt ccaagctagg cccccgctag gagtgtgaaa tgcccaacat 60

acctgccagg acaaccatag gacaagagac gagtacgtcg taggttcc 108

<210> 566

<211> 108

<212> DNA

<213> Artificial sequence

<400> 566

gactacatgg gacattgttc tgatgaaaac atgacgagta agttcactga cctggtttca 60

caaggcctga ctttgattcc ttcttatatc gagtacgtcg taggttcc 108

<210> 567

<211> 108

<212> DNA

<213> Artificial sequence

<400> 567

gactacatgg gacataaatg attcactctt ggccatatgg ccaatctgcg gagagaaaat 60

atgaagtaga taaacagaaa gtagtataaa aaatacgtcg taggttcc 108

<210> 568

<211> 108

<212> DNA

<213> Artificial sequence

<400> 568

gactacatgg gacatcctct ggcactgtga tcttccacac acaattcaga ttgttgtcat 60

aaggctcagg gtatccaggt gacaaaatag tcctacgtcg taggttcc 108

<210> 569

<211> 108

<212> DNA

<213> Artificial sequence

<400> 569

gactacatgg gacatcttga ctttacatct gtgaatacaa aaattaggag caagcctccc 60

aatcctaaac catatccagg tcagtaaggt acatacgtcg taggttcc 108

<210> 570

<211> 108

<212> DNA

<213> Artificial sequence

<400> 570

gactacatgg gacatctact gccttagggt cttttgtgag catgactata aagaaaagtt 60

gcctaaaggc aacccctgta gcaaccacct ccatacgtcg taggttcc 108

<210> 571

<211> 108

<212> DNA

<213> Artificial sequence

<400> 571

gactacatgg gacataattc tatccaaaaa ccagtgtgac tttcatagtt ggaggcagta 60

attcactgag taatcacaat ctaatgttcc ttttacgtcg taggttcc 108

<210> 572

<211> 108

<212> DNA

<213> Artificial sequence

<400> 572

gactacatgg gacatcactg attgggaata cattaccaag tagaggtgct gaataaacct 60

tgcgctgatt tcacataatg gctttgacta acatacgtcg taggttcc 108

<210> 573

<211> 108

<212> DNA

<213> Artificial sequence

<400> 573

gactacatgg gacatctgct agatctagca ggctacctgt ctggtactgg gaccttggtt 60

ttaacatgaa tataagaatt cacatttcat ttctacgtcg taggttcc 108

<210> 574

<211> 108

<212> DNA

<213> Artificial sequence

<400> 574

gactacatgg gacatgctcc aggatatatt cactttatgt ctttgaaaag ccacccagct 60

tcttggagtg ggagaatcac atgtataatc cagtacgtcg taggttcc 108

<210> 575

<211> 108

<212> DNA

<213> Artificial sequence

<400> 575

gactacatgg gacatcagag aagacaactt tgactctatg gatatttcta caattttttt 60

tgagataaaa tgtaccattg agaagctccc ccttacgtcg taggttcc 108

<210> 576

<211> 108

<212> DNA

<213> Artificial sequence

<400> 576

gactacatgg gacatatccc agaagtggct gcgcgcgggg taccccacaa tcctctcagg 60

gaagctctgc cacactgtga aggcgaaatc cactacgtcg taggttcc 108

<210> 577

<211> 108

<212> DNA

<213> Artificial sequence

<400> 577

gactacatgg gacatatctg agctatgtgg tggtggcagt ggtgtgtgtg tgtgcgcgcg 60

cacgcgtgta tgtgcatgca catgcacaca tggtacgtcg taggttcc 108

<210> 578

<211> 108

<212> DNA

<213> Artificial sequence

<400> 578

gactacatgg gacatcatta acagtttcca ccacctcaaa aatgatcaac aacccgaaaa 60

gcttggaacc aggtgcagac aggctaccag acttacgtcg taggttcc 108

<210> 579

<211> 108

<212> DNA

<213> Artificial sequence

<400> 579

gactacatgg gacattgcag gtggtgagga gcacaacgtc ctccccgtta tggacgtgat 60

ataaggcatt cctggtgtct gatcctatcc ctgtacgtcg taggttcc 108

<210> 580

<211> 108

<212> DNA

<213> Artificial sequence

<400> 580

gactacatgg gacatcacgt cagtctctaa ccgtgatcct aactgcctgg tcacccacac 60

acaaaaagaa taaaggctga gccagctggt aaatacgtcg taggttcc 108

<210> 581

<211> 108

<212> DNA

<213> Artificial sequence

<400> 581

gactacatgg gacattagct gttgagccat atgaagtttg agttccaatc ttatttccat 60

ttggaggtgt aggtagttct ccacaggaaa taatacgtcg taggttcc 108

<210> 582

<211> 108

<212> DNA

<213> Artificial sequence

<400> 582

gactacatgg gacatctcta ttccatccag gtcttccatc atctcccatg atacaggtga 60

gtgttgaata accttgaaga acataaccag cattacgtcg taggttcc 108

<210> 583

<211> 108

<212> DNA

<213> Artificial sequence

<400> 583

gactacatgg gacatttatt ctcttgacaa acaatggatt tctctccaat taattcaaac 60

ccaaactggc attcaaacct taaaacatca cgatacgtcg taggttcc 108

<210> 584

<211> 108

<212> DNA

<213> Artificial sequence

<400> 584

gactacatgg gacatttaca aactttctat ttctaagggg aatctaaaaa ataccactag 60

catctagttg aggagagcag gccccacagg ttgtacgtcg taggttcc 108

<210> 585

<211> 108

<212> DNA

<213> Artificial sequence

<400> 585

gactacatgg gacatttgca ccatttggat atccatatgg aaaaccaggg ctttctatag 60

tgccattaag tccttttaaa gttccaccac atgtacgtcg taggttcc 108

<210> 586

<211> 108

<212> DNA

<213> Artificial sequence

<400> 586

gactacatgg gacattgtcg ggaaatctct tcttccactt gggtaataaa atcacatttg 60

gtacatgagt gttctttgct ttccttgaca gactacgtcg taggttcc 108

<210> 587

<211> 108

<212> DNA

<213> Artificial sequence

<400> 587

gactacatgg gacatacggc accttctgtc aggcggcgct gcggggccgc atccagctgc 60

gggagcagct gccccgctac ctcatggggg agatacgtcg taggttcc 108

<210> 588

<211> 108

<212> DNA

<213> Artificial sequence

<400> 588

gactacatgg gacatcaatt ggcctctcat gtgttggtgg aacacacagg aaggtttttt 60

cttggcgata aggagaccat catctttcta tcatacgtcg taggttcc 108

<210> 589

<211> 108

<212> DNA

<213> Artificial sequence

<400> 589

gactacatgg gacatgaggg gggctttaaa cctgaaataa aaaggagagt agagcctaat 60

gaagcgctgg aaagcaagac ttagaaaatt acttacgtcg taggttcc 108

<210> 590

<211> 108

<212> DNA

<213> Artificial sequence

<400> 590

gactacatgg gacatggcac agaagagctc tgttcatata catggcacac cctcatgtta 60

aatctttggt tattccagaa tggatccttc taatacgtcg taggttcc 108

<210> 591

<211> 108

<212> DNA

<213> Artificial sequence

<400> 591

gactacatgg gacatataca cttgattccc agctacagac aggtaaaatg ttctgtctct 60

ccaactatgg ccaaacgatt tagcagtttg ttctacgtcg taggttcc 108

<210> 592

<211> 108

<212> DNA

<213> Artificial sequence

<400> 592

gactacatgg gacattgttt aatgtgtctt tgctactcat ccttcccttc atttccctca 60

aacacctcat aactatgact aggtcactat aaatacgtcg taggttcc 108

<210> 593

<211> 108

<212> DNA

<213> Artificial sequence

<400> 593

gactacatgg gacattcgta ggcattggct tgggtttttg tttttatttc tttttttttt 60

aatctactac tggacatcat ggacagtaca acctacgtcg taggttcc 108

<210> 594

<211> 108

<212> DNA

<213> Artificial sequence

<400> 594

gactacatgg gacataattg tgcagtcgta ttttctgcag aagccctcct accactaatg 60

tattaacagt ctcttaccct gccccgggga atttacgtcg taggttcc 108

<210> 595

<211> 108

<212> DNA

<213> Artificial sequence

<400> 595

gactacatgg gacatacgag atgtcccttg ttccctcctg ggcccctccc ttctccaaac 60

cctaacatcc atatgccacg ccttaggcag gcatacgtcg taggttcc 108

<210> 596

<211> 108

<212> DNA

<213> Artificial sequence

<400> 596

gactacatgg gacatgagaa aggaaggaga gaatcagaat ctcggtgtca gaagacaaac 60

ttgccaactt cataattttc catagggctg gcctacgtcg taggttcc 108

<210> 597

<211> 108

<212> DNA

<213> Artificial sequence

<400> 597

gactacatgg gacatgacgt gatataaggc attcctggtg tctgatccta tccctgtcag 60

gtacctcttc cccttgaata ccagcatgta ctttacgtcg taggttcc 108

<210> 598

<211> 108

<212> DNA

<213> Artificial sequence

<400> 598

gactacatgg gacattccaa gccaaaattc agtggagttg aaaagattaa gaccattggc 60

agcacataca tggcagcaac aggtctgagc gcttacgtcg taggttcc 108

<210> 599

<211> 108

<212> DNA

<213> Artificial sequence

<400> 599

gactacatgg gacattgcgg gcactgcttc aatgacacaa ctacaatgtg aaagtctaag 60

agttcttatt agcacttacc caccctgggg tgttacgtcg taggttcc 108

<210> 600

<211> 108

<212> DNA

<213> Artificial sequence

<400> 600

gactacatgg gacatacctc ctgtgagttg ggttgaagca gaggagtcac aatctcaaca 60

gtcagccaag agattaaagc caaaacacct acatacgtcg taggttcc 108

<210> 601

<211> 15

<212> DNA

<213> Artificial sequence

<400> 601

gactacatgg gacat 15

<210> 602

<211> 15

<212> DNA

<213> Artificial sequence

<400> 602

ggaacctacg acgta 15

<210> 603

<211> 50

<212> DNA

<213> Artificial sequence

<400> 603

aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct 50

<210> 604

<211> 66

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (25)..(32)

<223> n is a, c, g, or t

<400> 604

caagcagaag acggcatacg agatnnnnnn nngtgactgg agttcagacg tgtgctcttc 60

cgatct 66

<210> 605

<211> 21

<212> DNA

<213> Artificial sequence

<400> 605

aatgatacgg cgaccaccga g 21

<210> 606

<211> 21

<212> DNA

<213> Artificial sequence

<400> 606

caagcagaag acggcatacg a 21

<210> 607

<211> 42

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (34)..(41)

<223> n is a, c, g, or t

<400> 607

acactctttc cctacacgac gctcttccga tctnnnnnnn nt 42

<210> 608

<211> 29

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(8)

<223> n is a, c, g, or t

<400> 608

nnnnnnnnag atcggaagag cacacgtct 29

Claims (7)

1. A kit for detecting whether a fetus to be tested has a chromosome microdeletion microreplication syndrome as shown in the following table, comprising a probe set;

the probe set comprises 600 specific probes; each specific probe comprises a DNA fragment 2;

the DNA fragment 2 is a part of a chromosome region corresponding to the chromosome microdeletion and microduplication syndrome shown in the following table;

chromosomal microdeletion microduplication synthesisName of sign Located chromosome Corresponding chromosomal region 1p36 deficiency syndrome Chr1 10001-12840259 Cat cry syndrome Chr5 10001-12533304 Peroneal muscular atrophy Chr17 14097915-15470903 Digeorge syndrome Chr22 19009792-21452445 Du's muscular dystrophy ChrX 31135345-33231673 Williams-Berren syndrome Chr7 72744455-74142672 Wolf-Hirschhorn syndrome Chr4 492989-1983934 15q13.3 microdeletion syndrome Chr15 30910306-32445407 Miller-Dieker syndrome Chr17 1-2588909 Smith-Magenis syndrome Chr17 16773072-20222149 Syndrome of angel's foot Chr15 22749354-28438266 Langer-Giedion syndrome Chr8 108776506-121312414

；

The DNA fragment 2 is a single-stranded DNA molecule consisting of 78 nucleotides;

the nucleotide sequences of the DNA fragment 2 of the 600 specific probes are sequentially shown as 16 th to 93 th sites from the 5 'tail end of the sequence 1 in the sequence table to 16 th to 93 th sites from the 5' tail end of the sequence 600 in the sequence table.

2. The kit of claim 1, wherein: each specific probe also comprises a DNA fragment 1 and a DNA fragment 3; the DNA segment 1 is positioned at the 5' end of the DNA segment 2; the DNA fragment 3 is positioned at the 3' end of the DNA fragment 2;

the nucleotide sequence of the DNA fragment 1 is shown as a sequence 601 in a sequence table;

the nucleotide sequence of the DNA fragment 3 is shown as a sequence 602 in a sequence table;

the DNA segment 1 and/or the DNA segment 3 has a biotin label.

3. The kit of claim 1, wherein: the kit also comprises a primer pair A; the primer pair A consists of a primer 1 and a primer 2; the primer 1 comprises the DNA fragment 1; the primer 2 comprises the DNA fragment 3;

the nucleotide sequence of the primer 1 is shown as a sequence 601 in a sequence table;

the nucleotide sequence of the primer 2 is shown as a sequence 602 in a sequence table;

the primer 1 and/or the primer 2 has a biotin label.

4. The kit of claim 1, wherein: the kit also comprises a joint 1, a joint 2, a primer 3, a primer 4, a primer 5 and a primer 6;

the nucleotide sequence of the linker 1 is shown as a sequence 607 in a sequence table;

the nucleotide sequence of the linker 2 is shown as a sequence 608 in a sequence table;

the nucleotide sequence of the primer 3 is shown as a sequence 603 in a sequence table;

the nucleotide sequence of the primer 4 is shown as a sequence 604 in a sequence table;

the nucleotide sequence of the primer 5 is shown as a sequence 605 in a sequence table;

the nucleotide sequence of the primer 6 is shown as a sequence 606 in a sequence table.

5. The kit of claim 4, wherein: the 3' ends of the joint 1, the primer 5 and the primer 6 are all subjected to thio modification; the 5' end of the linker 2 is modified by phosphorylation.

6. The kit of any one of claims 1 to 5, wherein: the kit also comprises a carrier recorded with a judgment standard;

the judgment criteria are as follows: firstly, according to a formula (1), measuring the Z-score value of a certain area of a pregnant woman sample;

z-score: the Z-score value of a certain area of a pregnant woman sample to be detected;

readssample: the areas of the pregnant woman samples to be detected are normalized reads;

mean reads reference: referring to the average value of the reads of the region in the database;

s.d. reads reference: the standard deviation of reads of the reference database;

then, the following judgment is made: if the Z-score value of a certain area of the pregnant woman sample to be detected is larger than 3, the area is repeated; if the Z-score value of a certain area of the pregnant woman sample to be detected is less than or equal to 3, the area is normal; if the z-score value of a certain area of the pregnant woman sample to be detected is less than-3, the area is absent; if a region is duplicated or deleted, the fetus suffers from the corresponding chromosome microdeletion and microduplication syndrome; if a region is normal, the fetus does not have the corresponding chromosome microdeletion and microduplication syndrome;

the reference database consists of data of M healthy pregnant women; m is a natural number of 100 or more;

the steps of obtaining data for each healthy pregnant woman are as follows:

(K1) preparing a plasma free DNA library of a healthy pregnant woman; the linkers for preparing the plasma free DNA library of healthy pregnant women are the linker 1 and the linker 2 in claim 4;

(K2) after the step (K1) is completed, hybridizing the plasma free DNA library of the healthy pregnant woman with the probe set of any one of claims 1 or 2 to obtain a hybridization product;

(K3) after step (K2) is completed, collecting the target sequence from the hybridization product to obtain a target sequence capture library;

(K4) performing high-throughput sequencing on the target sequence capture library obtained in the step (K3) to obtain sequencing original data;

(K5) cutting off an adaptor sequence and low-quality bases from the sequencing original data obtained in the step (K4), filtering, and aligning to a corresponding position of the hg19 of the reference genome;

(K6) after the step (K5) is completed, removing repeated sequences caused by PCR amplification preference to obtain the data of the healthy pregnant woman;

dividing the reference database into regions, calculating the number of reads of each region, and homogenizing samples based on the median of the reads of each region; and then calculating the average value and the standard deviation of each region ready in the reference database, namely respectively obtaining the average value of the region ready in the reference database and the standard deviation of the region ready in the reference database.

7. A set of probes as claimed in claim 1 or 2.

Images