CN109627192B - Cyclonerane type sesquiterpene derivative and preparation and application thereof - Google Patents
Cyclonerane type sesquiterpene derivative and preparation and application thereof Download PDFInfo
- Publication number
- CN109627192B CN109627192B CN201910007382.3A CN201910007382A CN109627192B CN 109627192 B CN109627192 B CN 109627192B CN 201910007382 A CN201910007382 A CN 201910007382A CN 109627192 B CN109627192 B CN 109627192B
- Authority
- CN
- China
- Prior art keywords
- cyclonerane
- dichloromethane
- culture medium
- sesquiterpene derivative
- petroleum ether
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C291/00—Compounds containing carbon and nitrogen and having functional groups not covered by groups C07C201/00 - C07C281/00
- C07C291/02—Compounds containing carbon and nitrogen and having functional groups not covered by groups C07C201/00 - C07C281/00 containing nitrogen-oxide bonds
- C07C291/04—Compounds containing carbon and nitrogen and having functional groups not covered by groups C07C201/00 - C07C281/00 containing nitrogen-oxide bonds containing amino-oxide bonds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/28—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group; Thio analogues thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to the field of algae inhibitors, in particular to a cyclonerane sesquiterpene derivative derived from algae endophytic fungi, a preparation method thereof and application thereof in algae inhibition. The structure of the cyclonerane sesquiterpene derivative is shown in the formula (I), the preparation method comprises the steps of inoculating seaweed endophytic fungus Trichoderma asperellum A-YMD-9-2 into a fungus culture medium for fermentation culture, and separating and purifying the fermentation product to obtain the cyclonerane sesquiterpene derivative shown in the formula (I). The semiinhibitory concentration of the compound on microalgae obtained by the nerolidine sesquiterpene derivative through a microalgae inhibition activity experiment can reach 2.1 micrograms/ml.
Description
Technical Field
The invention relates to the field of algae inhibitors, in particular to a cyclonerane sesquiterpene derivative derived from algae endophytic fungi, a preparation method thereof and application thereof in algae inhibition.
Background
Worldwide, offshore red tide disasters are increasingly aggravated, and the development of marine fishery, offshore tourism and marine economy is seriously hindered. After 2000, China enters the high-incidence period of red tide, and the incidence frequency and the harm degree of the red tide are obviously increased. The main harm forms of red tide are four, namely, the ecological balance of the ocean is destroyed; secondly, marine fishery resources are damaged; thirdly, the health of human beings is harmed; and fourthly, the development of marine tourism industry is influenced. Statistically, the economic loss caused by red tide is more than 10 billion yuan each year. There are about 300 species of microalgae in the ocean that can trigger red tide, and among them, there are about 80 species of toxic red tide algae. The scientific and effective prevention and treatment of red tide is one of the worldwide problems.
At present, the prevention and treatment strategy of red tide is to prevent the eutrophication of seawater, and also to inhibit the development of red tide organisms, namely red tide algae, and the method for inhibiting the development of the red tide algae is mainly chemical treatment. Although chemical agents such as copper sulfate, chlorine gas, etc. rapidly and effectively kill or inhibit harmful algae or inhibit their growth and reproduction, there are problems of harm to non-red tide organisms, drug residues, secondary pollution to the environment, etc.
Compared with the traditional chemical agents used in the treatment of the red tide, the marine organism-derived natural drug has the advantages of strong pertinence, high safety, obvious activity and environmental friendliness, and provides a new idea for solving the problem of prevention and treatment of the red tide caused by red tide algae at present
Disclosure of Invention
The invention aims to provide a cyclonerane sesquiterpene derivative and preparation and application thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
a cyclonerane sesquiterpene derivative has a structure shown in formula (I)
A preparation method of cyclonerane sesquiterpene derivatives comprises inoculating Trichoderma asperellum A-YMD-9-2 into fungus culture medium, fermenting, and purifying the fermentation product to obtain cyclonerane sesquiterpene derivatives shown in formula (I); the trichoderma asperellum A-YMD-9-2 is preserved in a China Center for Type Culture Collection (CCTCC) in 2018, 6 months and 27 days, and the preservation number is CCTCC M2018405.
The method specifically comprises the following steps:
1) inoculating Trichoderma asperellum A-YMD-9-2 into a fungus culture medium, fermenting for 10-60 days, extracting with an organic solvent, and concentrating to obtain a crude extract;
2) subjecting the crude extract obtained in the step 1) to silica gel column chromatography, performing gradient elution by using an eluent, collecting an elution component, and detecting the elution component by using thin layer chromatography;
3) collecting the eluted components in the step 2), and sequentially separating and purifying by reversed phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain the cyclonerane sesquiterpene derivative shown in the formula (I).
The fungus culture medium in the step 1) is a rice solid culture medium, a jerusalem artichoke glucose liquid culture medium or a potato glucose liquid culture medium.
In the step 1), the organic solvent extracting solution is one or more of ethyl acetate, petroleum ether, n-hexane, cyclohexane, dichloromethane, methanol, ethanol, propanol or isopropanol.
The organic solvent in the step 2) is one or more of petroleum ether-ethyl acetate, petroleum ether-ethanol, petroleum ether-propanol, petroleum ether-isopropanol, dichloromethane-ethyl acetate, dichloromethane-methanol, dichloromethane-ethanol, dichloromethane-propanol and dichloromethane-isopropanol in a volume ratio of 50-0: 1.
The reverse phase silica gel column chromatography eluent in the step 3) is water-methanol or water-ethanol with the volume ratio of 5-0: 1; the gradient of the semi-preparative high performance liquid chromatography eluent is water-methanol or water-acetonitrile with the volume ratio of 10-0: 1.
The application of the cyclonerane sesquiterpene derivative is used for preparing an algistat for microalgae.
The microalgae are marine dunaliella anatipestifer causing red tide hazards.
The invention has the following advantages: the cyclonerolidine sesquiterpene derivative is obtained by fermenting and separating Trichoderma asperellum A-YMD-9-2 separated from marine red algae gracilaria to obtain the cyclonerolidine sesquiterpene derivative, and the half inhibition concentration of the compound on marine dunaliella caused by red tide is 2.1 microgram/milliliter through a microalgae inhibition activity experiment.
The specific implementation mode is as follows:
the invention is further illustrated below with reference to the examples of embodiment.
Example 1
The structure of the cyclonerane sesquiterpene derivative derived from the seaweed endophytic fungi is shown as a formula (I).
The compound has the following physicochemical and spectral characteristics:
colorless oil; specific optical rotation [ alpha ]]20 D-81(c 0.70, MeOH); readily soluble in water and organic solvents; can carry out complex reaction with ferric iron, trivalent chromium, trivalent aluminum and divalent lead ions; nuclear magnetic resonance hydrogen spectrum (deuterium substituted methanol as solvent)H1.01d (6.8),1.57m,1.63m,1.51m,1.83m,1.58m,1.83m,1.46m,1.88m,1.82m,4.89m,4.98s,4.96s,1.22s,1.13s,1.76br s,6.31br s,2.38br t (6.6),3.72t (6.6),2.07br s; nuclear magnetic resonance carbon spectrum (deuterium substituted methanol as solvent)C 15.4(CH3),45.6(CH),82.0(C),41.4(CH2),25.2(CH2),56.2(CH),75.6(C),38.1(CH2),24.2(CH2),61.0(CH),144.7(C),113.5(CH2),26.1(CH3),24.3(CH3),21.2(CH3),170.1(C),118.2(CH),152.1(C),44.7(CH2),60.9(CH2),18.9(CH3) (ii) a High resolution mass spectrum [ M + Na]+m/z 406.2565, calculated 406.2569.
Example 2
The preparation method of the cyclonerane sesquiterpene derivative shown in the formula (I) comprises the following steps:
a Trichoderma asperellum A-YMD-9-2 strain growing well on a plate is taken, cut into small pieces and inoculated into a rice solid culture medium, 50 g of the rice solid culture medium is put into each 1L triangular flask, 200 bottles in total are statically fermented for 40 days at room temperature, then ethyl acetate is used for extraction for three times, reduced pressure concentration is carried out, and 212.4 g of crude extract is obtained after concentration.
The rice solid medium comprises 500 g of rice, 6 g of peptone, 500 ml of distilled water and 500 ml of aged seawater per liter.
Trichoderma asperellum A-YMD-9-2 strain was preserved in the China center for type culture Collection CCTCC at 2018, 6 months and 27 days, address: the preservation number of the university of Wuhan, China is CCTCC M2018405, the classification name is Trichoderma asperellum, and the plant number is A-YMD-9-2.
Subjecting the crude extract to 100-200 mesh silica gel column chromatography, gradient eluting sequentially with petroleum ether-ethyl acetate and dichloromethane-methanol at volume ratios of 50:1, 20:1, 10:1, 5:1, 2:1, 1: 1-0: 1, respectively collecting eluates, detecting the collected components by Thin Layer Chromatography (TLC) (dichloromethane-methanol development, anisaldehyde-sulfuric acid color development or 254nm ultraviolet light detection at volume ratio of 50-1: 1), and determining and combining the same or similar parts according to Rf value to obtain 8 components (1-8).
Component 7 with Rf value of 0.6-0.7 (volume ratio of 6:1 dichloromethane-methanol development, 254nm ultraviolet detection), that is, component eluted with volume ratio of 5:1 dichloromethane-methanol gradient, is sequentially subjected to reversed phase C18Silica gel column and semi-preparative high performance liquid chromatography. Inverse phase C18Performing silica gel column chromatography with water-methanol at volume ratio of 1:1, performing TLC detection (dichloromethane-methanol at volume ratio of 6:1 is developed, ultraviolet light at 254nm is detected, and anisaldehyde-sulfuric acid is developed), and collecting component with fluorescence under ultraviolet light at 254nm and anisaldehyde-sulfuric acid is developed into red; collecting the fractions, performing semi-preparative high performance liquid chromatography with water-acetonitrile gradient of 4-1.5:1 volume ratio for 30 min, and collecting the fraction with peak after 15 min to obtain compound (84.2 mg) of formula (I). Detecting by thin layer chromatography (volume ratio 6:1 dichloromethane-methanol development, 254nm ultraviolet detection, anisaldehyde-sulfuric acid color development), and determining as pure compound, wherein the spot is single, uniform fluorescence and red spot. The structure is identified as a cyclonerane sesquiterpene derivative through spectral analysis, and the structural formula is shown as (I).
Example 3
The difference from the embodiment 2 is that
A Trichoderma asperellum A-YMD-9-2 strain growing well on a flat plate is taken, cut into small pieces and inoculated into a jerusalem artichoke glucose liquid culture medium, 300 ml of the culture medium is put into each 1L triangular flask, 200 bottles in total are statically fermented for 40 days at room temperature, then dichloromethane is used for extraction for three times, reduced pressure concentration is carried out, and 200 g of crude extract is obtained after concentration.
The jerusalem artichoke glucose liquid culture medium comprises 500 ml of boiling juice containing 200 g of jerusalem artichoke tubers per liter, 20 g of glucose, 5 g of peptone and 500 ml of aged seawater.
Subjecting the crude extract to 200-300 mesh silica gel column chromatography, sequentially performing gradient elution with petroleum ether-ethanol at volume ratio of 50:1, 30:1, 15:1, 10:1, 5:1, 2:1, 1:1 to 0:1, respectively collecting eluates, detecting by thin layer chromatography (petroleum ether-ethanol at volume ratio of 50-0:1 is developed, anisaldehyde-sulfuric acid is developed or 254nm ultraviolet light is detected), and judging and combining the same or similar parts according to Rf value to obtain 8 components (1-8).
Developing the component 7 with Rf value of 0.6-0.7 (petroleum ether-ethanol ratio of 2:1, detecting with 254nm ultraviolet light, and developing with anisaldehyde-sulfuric acid), that is, eluting with petroleum ether-ethanol gradient of 2:1, sequentially passing through reversed phase C18Silica gel column and semi-preparative high performance liquid chromatography. Inverse phase C18Performing silica gel column chromatography with water-ethanol at volume ratio of 1:1, performing TLC detection (petroleum ether-ethanol development at volume ratio of 2:1, performing UV detection at 254nm, and performing color development on anisaldehyde-sulfuric acid), and collecting component with fluorescence under UV at 254nm and anisaldehyde-sulfuric acid component with red color; collecting the components, performing semi-preparative high performance liquid chromatography with water-methanol gradient of volume ratio of 4-1:1 for 30 min, and collecting 15 min peak components to obtain cyclonerane sesquiterpene derivatives shown in formula (I).
Example 4
Microalgae inhibition activity experiment:
specifically, the method comprises activating microalgae to be tested, collecting microalgae in logarithmic growth phase, and diluting with sterilized f/2 culture medium to a certain cell concentration (about 5 × 10)4One/ml), take 96-well plate, add 195 microliter algae solution per well as test culture plate. The sample group and the control group were each provided in triplicateSample group plus 5. mu.l of a 4 mg/ml solution of the compound in dimethyl sulfoxide (DMSO) as solvent, blank control group plus 5. mu.l of solvent, positive control group plus 5. mu.l of K at a 4 mg/ml concentration2Cr2O7And (3) solution. The culture temperature is 20 ℃, the illumination intensity is 2000Lux, and the light-dark ratio is 14:10 (h), and the culture is carried out for 24 h. Wherein the microalgae to be tested is marine dunaliella anata.
Counting microalgae in each experimental group under a microscope by using a blood counting plate. For the experimental group with the inhibition rate of more than 50%, the half inhibition concentration IC is further calculated50The value is obtained. The prepared compound solutions with final concentrations of 4 mg/ml were sequentially diluted to finally obtain 15 groups of sequentially reduced concentrations of components (1000, 500, 200, 100, 50, 20, 10, 5, 2, 1, 0.5, 0.25, 0.125, 0.0625, 0.03125 μ g/ml). Observing and calculating microalgae inhibition rate of the compound under each concentration gradient, taking at least 5 concentration gradients with the inhibition rate between 0 and 100, and calculating IC50The value is obtained.
The experimental results are as follows: the obtained cyclonerane sesquiterpene derivative has half inhibitory concentration of 2.1 microgram/ml on marine dunaliella and has effect of inhibiting marine dunaliella.
Claims (3)
2. A method for preparing the cyclonerane-type sesquiterpene derivatives of claim 1, which comprises the following steps: inoculating Trichoderma asperellum A-YMD-9-2 into a fungus culture medium for fermentation culture, and purifying the fermentation product to obtain the cyclonerane sesquiterpene derivative shown in formula (I);
the Trichoderma asperellum A-YMD-9-2 is preserved in a China Center for Type Culture Collection (CCTCC) in 2018, 6 months and 27 days, and the preservation number is CCTCC M2018405;
the preparation method comprises the following specific steps:
1) inoculating Trichoderma asperellum A-YMD-9-2 into a fungus culture medium, fermenting for 10-60 days, extracting with an organic solvent, and concentrating to obtain a crude extract;
2) subjecting the crude extract obtained in the step 1) to silica gel column chromatography, performing gradient elution by using an eluent, collecting an elution component, and detecting the elution component by using thin layer chromatography;
3) collecting the eluted components in the step 2), and sequentially separating and purifying by reversed phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain the cyclonerane sesquiterpene derivative shown in the formula (I);
the fungus culture medium in the step 1) is a rice solid culture medium, a jerusalem artichoke glucose liquid culture medium or a potato glucose liquid culture medium;
the organic solvent in the step 1) is one or more of ethyl acetate, petroleum ether, n-hexane, cyclohexane, dichloromethane, methanol, ethanol, propanol or isopropanol;
the eluent in the step 2) is one or more of petroleum ether-ethyl acetate, petroleum ether-ethanol, petroleum ether-propanol, petroleum ether-isopropanol, dichloromethane-ethyl acetate, dichloromethane-methanol, dichloromethane-ethanol, dichloromethane-propanol and dichloromethane-isopropanol in a volume ratio of 50-0: 1;
the eluent of the reverse phase silica gel column chromatography in the step 3) is water-methanol or water-ethanol with the volume ratio of 5-0: 1; the gradient of the semi-preparative high performance liquid chromatography eluent is water-methanol or water-acetonitrile with the volume ratio of 10-0: 1.
3. Use of the cyclonerane-type sesquiterpene derivatives of claim 1 wherein: the cyclonerane sesquiterpene derivative is used for preparing an algistat for microalgae;
the microalgae are marine dunaliella anatipestifer causing red tide hazards.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910007382.3A CN109627192B (en) | 2019-01-04 | 2019-01-04 | Cyclonerane type sesquiterpene derivative and preparation and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910007382.3A CN109627192B (en) | 2019-01-04 | 2019-01-04 | Cyclonerane type sesquiterpene derivative and preparation and application thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109627192A CN109627192A (en) | 2019-04-16 |
CN109627192B true CN109627192B (en) | 2020-12-29 |
Family
ID=66057806
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910007382.3A Active CN109627192B (en) | 2019-01-04 | 2019-01-04 | Cyclonerane type sesquiterpene derivative and preparation and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109627192B (en) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101225078A (en) * | 2008-01-18 | 2008-07-23 | 中国科学院海洋研究所 | Sea weed sesquiterpenoids as well as preparation method and application thereof |
CN101787008A (en) * | 2009-01-23 | 2010-07-28 | 中国科学院上海药物研究所 | N-acetyl neuraminic acid compounds, medicine compositions thereof and preparation methods and purposes thereof |
CN102659521A (en) * | 2012-04-09 | 2012-09-12 | 中国科学院烟台海岸带研究所 | Alga bromosesquiterpene compound, preparation thereof and application thereof |
FR3012725A1 (en) * | 2013-11-07 | 2015-05-08 | Ard Sa | COMPOSITIONS OF BIOLOGICAL CONTROL AGENTS, PROCESS FOR THEIR PREPARATION AND USES THEREOF |
CN107668374A (en) * | 2017-10-03 | 2018-02-09 | 长沙仲善新能源科技有限公司 | A kind of single cell protein biological feedstuff and preparation method thereof |
CN108409539A (en) * | 2018-04-23 | 2018-08-17 | 江苏省海洋资源开发研究院(连云港) | A kind of preparation method and purposes of Porphyra yezoensis sesquiterpenoids algistatic activity compound |
CN108467337A (en) * | 2018-04-23 | 2018-08-31 | 中国科学院烟台海岸带研究所 | A kind of sesquiterpene alcohols compound and its preparation and application |
-
2019
- 2019-01-04 CN CN201910007382.3A patent/CN109627192B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101225078A (en) * | 2008-01-18 | 2008-07-23 | 中国科学院海洋研究所 | Sea weed sesquiterpenoids as well as preparation method and application thereof |
CN101787008A (en) * | 2009-01-23 | 2010-07-28 | 中国科学院上海药物研究所 | N-acetyl neuraminic acid compounds, medicine compositions thereof and preparation methods and purposes thereof |
CN102659521A (en) * | 2012-04-09 | 2012-09-12 | 中国科学院烟台海岸带研究所 | Alga bromosesquiterpene compound, preparation thereof and application thereof |
FR3012725A1 (en) * | 2013-11-07 | 2015-05-08 | Ard Sa | COMPOSITIONS OF BIOLOGICAL CONTROL AGENTS, PROCESS FOR THEIR PREPARATION AND USES THEREOF |
CN107668374A (en) * | 2017-10-03 | 2018-02-09 | 长沙仲善新能源科技有限公司 | A kind of single cell protein biological feedstuff and preparation method thereof |
CN108409539A (en) * | 2018-04-23 | 2018-08-17 | 江苏省海洋资源开发研究院(连云港) | A kind of preparation method and purposes of Porphyra yezoensis sesquiterpenoids algistatic activity compound |
CN108467337A (en) * | 2018-04-23 | 2018-08-31 | 中国科学院烟台海岸带研究所 | A kind of sesquiterpene alcohols compound and its preparation and application |
Non-Patent Citations (3)
Title |
---|
New fungitoxic sesquiterpenoids, chokols A-G, from stromata of Epichloe typhina and the absolute configuration of chokol E;Koshino Hiroyuki et al;《Agricultural and Biological Chemistry》;19891231;第53卷(第3期);789-796 * |
Tricholumin A, a Highly Transformed Ergosterol Derivative from the Alga-Endophytic Fungus Trichoderma asperellum;Yin Ping Song et al;《Organic letters》;20180926;第20卷;6306-6309 * |
海藻内生真菌棘孢木霉dl-34中的二萜和甾体化合物;梁小蕊等;《化学与生物工程》;20161231;第33卷(第5期);32-36 * |
Also Published As
Publication number | Publication date |
---|---|
CN109627192A (en) | 2019-04-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108467337B (en) | Sesquiterpene enol compound and preparation and application thereof | |
CN109503414B (en) | Nitrogenous ring nerolidine type sesquiterpene derivative and preparation and application thereof | |
CN108383707B (en) | Carotene sesquiterpene compound and preparation and application thereof | |
CN108484363B (en) | Sesquiterpene triol compound and preparation and application thereof | |
CN109503535B (en) | Bicyclic ring nerolidine type sesquiterpene derivative and preparation and application thereof | |
CN107586721B (en) | Benzophenone compound with antioxidant activity and preparation method and application thereof | |
CN108373457B (en) | Epoxy sesquiterpenoids and preparation and application thereof | |
CN109503428B (en) | Cyclonerane hydroxamic acid derivative and preparation and application thereof | |
CN109988180B (en) | Diketopiperazine derivative and preparation and application thereof | |
CN109503413B (en) | Monocyclic ring nerolidine type sesquiterpene derivative and preparation and application thereof | |
CN109956883B (en) | Acetylated nitrogenous ring nerolidine type sesquiterpene derivative and preparation and application thereof | |
CN112111409A (en) | Penicillium oxalicum and application thereof | |
CN109627192B (en) | Cyclonerane type sesquiterpene derivative and preparation and application thereof | |
CN108220357B (en) | Application and preparation method of pyrrole-2-carboxylic acid | |
CN109678749B (en) | Cyclonerane type sesquiterpene amide and preparation and application thereof | |
CN110003041B (en) | Cyclonerane acylated derivative and preparation and application thereof | |
CN108467398B (en) | Diketopiperazine compound and preparation and application thereof | |
CN111635316B (en) | Chlorine-containing sesquiterpene derivative and preparation and application thereof | |
CN111662257B (en) | Benzencadinane derivative, and preparation and application thereof | |
CN111606878B (en) | Cadinane derivative and preparation and application thereof | |
CN108640832B (en) | Cadinane sesquiterpenoids, and preparation and application thereof | |
CN113444131A (en) | N-acetylglucosamine compounds, and preparation method and application thereof | |
CN110951619A (en) | Anthraquinone compound with anti-helicobacter pylori activity and application thereof | |
Stoermer et al. | A new distribution record for Hymenomonas roseola Stein (Prymnesiophyceae, Coccolithophoraceae) and Spiniferomonas trioralis Takahashi (Chrysophyceae, Synuraceae) in the Laurentian Great Lakes | |
CN108586218A (en) | A kind of chloro-cyclopentene ketone compounds and its preparation and application |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |