CN109625635B

CN109625635B - Low temperature storage device

Info

Publication number: CN109625635B
Application number: CN201910150161.1A
Authority: CN
Inventors: 郭福军
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-02-28
Filing date: 2019-02-28
Publication date: 2020-01-21
Anticipated expiration: 2039-02-28
Also published as: CN109625635A

Abstract

The invention discloses a low-temperature storage device, comprising: the device comprises an outer tank, a shaft assembly, an inner container arranged in the outer tank and a driving assembly arranged outside the outer tank; the inner container is fixedly connected with the outer container, shaft holes for penetrating the shaft assemblies are respectively formed in the top of the outer container and the center of the top of the inner container, the shaft holes in the top of the outer container and the shaft holes in the top of the inner container are connected through corrugated pipes, and a first heat insulation assembly for blocking air flow convection is arranged between each corrugated pipe and each shaft assembly; an inner rotary body for placing biological samples in a partition mode is arranged on the shaft assembly positioned in the inner container, and the shaft assembly positioned outside the outer tank is connected with the driving assembly; the eccentric department that outer jar top and inner bag top correspond sets up respectively as getting the thing hole of getting to put biological sample passageway, sets up the heat preservation lid that can open between two thing holes of getting. The low-temperature storage device effectively reduces the liquid nitrogen loss and has better effect of storing the biological sample.

Description

Low temperature storage device

Technical Field

The invention relates to the technical field of low-temperature storage, in particular to a low-temperature storage device.

Background

In the field of biological medicine, biological tissues such as blood, stem cells, vaccines and the like can be preserved for long-term activity by low-temperature storage in a liquid nitrogen environment, biological samples are usually placed in a liquid nitrogen tank in a partitioning mode, and when the biological samples are used, an operator opens a fetching hole to fetch the biological samples to be fetched from the liquid nitrogen tank.

When the existing liquid nitrogen tank is used, most operators need to stretch hands into the liquid nitrogen tank through the object taking hole to stir samples to place partitions, and when the samples to be extracted are rotated to the position below the object taking hole, the samples are taken out. The mode is characterized by multiple manual sampling operations and long time consumption, so that not only can frostbite easily occur, but also larger liquid nitrogen loss can be caused. There is also a liquid nitrogen tank which uses an external motor driving method to reduce manual sampling operation, but this method generates additional liquid nitrogen loss due to the introduction of external driving connection inside and outside the liquid nitrogen tank.

Disclosure of Invention

The invention provides a low-temperature storage device, which is used for overcoming the technical problems in the prior art, not only reducing manual sampling operation, but also reducing extra liquid nitrogen loss.

The invention provides a low-temperature storage device, comprising: the device comprises an outer tank, a shaft assembly, an inner container arranged in the outer tank and a driving assembly arranged outside the outer tank; the inner container is fixedly connected with the outer container, shaft holes for penetrating the shaft assemblies are respectively formed in the top of the outer container and the center of the top of the inner container, the shaft holes in the top of the outer container and the shaft holes in the top of the inner container are connected through corrugated pipes, and a first heat insulation assembly for blocking air flow convection is arranged between each corrugated pipe and each shaft assembly; an inner rotary body for placing biological samples in a partition mode is arranged on the shaft assembly positioned in the inner container, and the shaft assembly positioned outside the outer tank is connected with the driving assembly; the eccentric parts corresponding to the top of the inner container and the top of the outer tank are respectively provided with an object taking hole used as a biological sample taking and placing channel, and an openable heat preservation cover is arranged between the two object taking holes; the shaft assembly includes: the first shaft and the second shaft are connected together through a second heat insulation assembly, the first shaft is connected with the driving assembly, and an inner rotating body is mounted on the second shaft; the first shaft is of a multi-layer sleeve structure, the diameter of each layer of sleeve is different from the diameters of other layers of sleeves, adjacent layers of sleeves of the multi-layer sleeves are sequentially connected end to end, when the free end of the innermost layer of sleeve of the multi-layer sleeves is connected with the driving assembly, the free end of the outermost layer of sleeve is connected with the second shaft, or when the free end of the outermost layer of sleeve of the multi-layer sleeves is connected with the driving assembly, the free end of the innermost layer of sleeve is connected with the second shaft.

According to the invention, the driving assembly is arranged outside the outer tank, so that the volume in the tank is saved, and the storage position of a sample can be accurately positioned outside the tank, thereby reducing the sampling time and reducing the heat leakage; by adopting the corrugated pipe to connect the outer tank and the inner container so as to prolong the heat conduction path and arranging the first heat insulation assembly for blocking the convection of air flow between the corrugated pipe and the shaft assembly, the heat leakage can be reduced, thereby effectively reducing the loss of liquid nitrogen and ensuring that the low-temperature storage device has better effect of storing biological samples.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a low temperature storage device according to an embodiment of the present invention;

FIG. 2 is a schematic view of the shaft assembly of FIG. 1;

FIG. 3 is a partially enlarged view of the connection between the outer vessel and the inner container of FIG. 1 through the shaft assembly;

FIG. 4 is a schematic view of the first shaft of FIG. 1;

FIG. 5 is an enlarged view of a portion of the object hole in FIG. 1.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the technical solution of the present invention clearer, embodiments of the present invention are described in detail below with reference to the accompanying drawings.

Fig. 1 is a schematic structural diagram of a low-temperature storage device according to an embodiment of the present invention, fig. 2 is a schematic structural diagram of a shaft assembly in fig. 1, fig. 3 is a partially enlarged view of a connection portion between an outer tank and an inner tank in fig. 1 where the shaft assembly penetrates, and as shown in fig. 1-3, the low-temperature storage device in the embodiment includes: an outer vessel 200, a shaft assembly 100, an inner container 300 installed inside the outer vessel, and a driving assembly 400 installed outside the outer vessel; the inner container 300 is fixedly connected with the outer tank 200, the center of the top of the outer tank is provided with a shaft hole 209, the center of the top of the inner container is provided with a shaft hole 302 which is respectively used for penetrating through a shaft assembly, the shaft hole 209 of the top of the outer tank is connected with the shaft hole 302 of the top of the inner container by adopting the corrugated pipe 30, and a first heat insulation assembly 31 for blocking air flow convection is arranged between the corrugated pipe 30 and the shaft assembly 100; an inner rotary body 101 for placing biological samples in a subarea way is arranged on the shaft assembly 100 positioned in the inner container 300, and the shaft assembly 100 positioned outside the outer tank 200 is connected with a driving assembly 400; the eccentric department that outer jar top and inner bag top correspond is provided with respectively and gets the thing hole as getting to put biological sample passageway, and two are got and are provided with the heat preservation lid 500 that can open between the thing hole.

The low temperature storage device in this embodiment adopts and sets up drive assembly 400 in outer jar outside and provides pivoted drive power for axle subassembly 100, and drive assembly places the volume in can saving jar on the one hand outside outer jar, and on the other hand can conveniently control the rotational position of the interior rotating body in jar outside outer jar, and the accurate location sample deposit position to reduce the sample time, reduce the heat leakage.

The driving component in the embodiment of the invention can be a hand wheel controlled manually or a motor, the angle of the inner rotary body can be accurately controlled by arranging the partition scale marks on the hand wheel, and the motor can be driven in an electric control mode to accurately control the rotation angle of the inner rotary body, so that the sampling operation time can be reduced.

In use, when need not the sample, the sealed thing hole of getting of heat preservation lid 500 blocks jar inside and outside heat conduction, when needs sample, will wait to sample the sample through drive assembly 400 control internal rotation 101 and rotate and get thing hole below, then open heat preservation lid 500 and take out the sample, cover heat preservation lid 500 again after the sample.

In the embodiment of the invention, the neck pipe at the joint between the outer tank penetrating through the shaft assembly 100 and the shaft hole of the inner container adopts a corrugated pipe connecting structure, the corrugated pipe connection lengthens a heat conducting path, which is beneficial to reducing heat leakage, and the first heat insulation assembly 31 for preventing gas convection is arranged between the shaft assembly 100 and the corrugated pipe 30, so that the heat leakage is further reduced.

According to the embodiment of the invention, the driving assembly is arranged outside the outer tank, so that the volume in the tank is saved, and the storage position of the sample can be accurately positioned outside the tank, thereby reducing the sampling time and reducing the heat leakage; by adopting the corrugated pipe to connect the outer tank and the inner container so as to prolong the heat conduction path and arranging the first heat insulation assembly for blocking the convection of air flow between the corrugated pipe and the shaft assembly, the heat leakage can be reduced, thereby effectively reducing the loss of liquid nitrogen and ensuring that the low-temperature storage device has better effect of storing biological samples.

In the concrete implementation, the first thermal-insulated subassembly 31 that sets up is for following the FRP pipe 12 and the polytetrafluoroethylene gasket 13 that axial interval set up, for transition fit between polytetrafluoroethylene gasket and the axle subassembly, guaranteeing under the prerequisite that the axle can rotate, the relative glass steel material of polytetrafluoroethylene material, the surface is more smooth, can reach and the axle between the clearance minimum to effectively reduce heat leakage.

In a specific implementation, a shaft assembly includes: the first shaft 10 is connected to the driving assembly 400, and the second shaft 20 is mounted with the inner rotary body 101 by the second heat insulation assembly, and the first shaft 10 is connected to the driving assembly 400.

In order to ensure certain strength and rigidity, the first shaft is a stainless steel shaft, and the second shaft is an aluminum or aluminum alloy shaft, so that a heat insulation assembly is required to be arranged at the joint between the two shafts for connection so as to reduce heat conduction between the second shaft positioned in the inner container and the first shaft connected with the outside.

The second heat insulation assembly comprises a first shaft sleeve 14 and a second shaft sleeve 21 which are connected through a first bolt 16, the first shaft sleeve 14 is sleeved at one end of the first shaft 10 and is fixedly connected with the first shaft 10 through the first bolt 16, and the second shaft sleeve 21 is sleeved at one end of the second shaft 20 and is fixedly connected with the second shaft 20 through a second bolt 23. In order to further reduce heat leakage, in the embodiment of the present invention, a heat insulation assembly 15 is disposed between the first bolt 16 and the second bushing 20 to reduce contact between the first bushing and the second bushing, and a heat insulation assembly 22 is disposed between the second bolt 23 and the second bushing 20 to reduce heat conduction between the second bushing and the second shaft, so as to reduce heat conduction from the second shaft to the first shaft.

In the embodiment of the invention, the first shaft sleeve 14 is in a boss structure, the bottom of the second shaft sleeve 21 is sleeved on the boss, the heat insulation pad 25 with corresponding thickness is arranged between the bottom of the first shaft sleeve 14 and the second shaft according to the size of the gap, and the first shaft sleeve and the second shaft sleeve are in line contact 24, so that the heat conduction between the first shaft sleeve and the second shaft sleeve can be reduced due to the line contact, the heat leakage is reduced, and the axial force can be transmitted.

In the above embodiment, under the condition of ensuring the strength requirement, the first shaft 10 adopts a multi-layer sleeve structure, fig. 4 is a schematic structural view of the first shaft in fig. 1, and as shown in fig. 4, adjacent layers of sleeves of the multi-layer sleeve are sequentially connected end to end, so that the heat conduction length is increased within a limited length, and the purpose of reducing heat transfer is achieved. In specific implementation, the steel pipe can be formed by sequentially welding three steel pipes with different diameters end to end. The free end of the outermost sleeve is connected to the second shaft when the free end of the innermost sleeve of the multi-layer sleeves is connected to the drive assembly, or the free end of the innermost sleeve of the multi-layer sleeves is connected to the second shaft when the free end of the outermost sleeve is connected to the drive assembly.

In practical use, if the motor is adopted to drive the shaft assembly to rotate, a coupling connection mode or a gear meshing connection mode can be adopted between the motor output shaft and the shaft assembly. In the embodiment of the invention, in order to make the power transmission more stable, the driving assembly 400 transmits the power to the first shaft 10 in a gear transmission mode, that is, the first gear 401 is installed on the output shaft of the driving assembly 400, the first shaft 10 is provided with the bearing seat 17, the bearing seat 17 is provided with the second gear 18 engaged with the first gear 401, and when the first shaft adopts a multi-layer sleeve structure, the bearing seat 17 is respectively connected with the free end of the multi-layer sleeve and the second gear. First gear 401 and second gear 18 are bevel gears, adopt the bevel gear transmission can make the axial of axle subassembly perpendicular to the axial of drive assembly's output shaft, and the axis of first gear intersects perpendicularly with the axis of second gear promptly, can change driven axial like this, and not only drive assembly can stabilize the installation at outer jar top, and the axle subassembly can reduce radial rocking moreover.

In use, when making the axle subassembly rotate, can also keep radial and axial firm, the fixed U type support piece 40 that sets up in shaft hole 209 department at outer jar top, the bottom of U type support piece 40 is equipped with the through-hole corresponding with shaft hole 209, so that wear to establish the axle subassembly, U type support piece's U type inslot installation thrust bearing 11, the primary shaft that will be connected with bearing frame 17 passes thrust bearing 11 in proper order, U type support piece's through-hole, last sealing member 42 presses on step 43 and U type support piece of bearing frame 17, seal thrust bearing in U type inslot, and connect the fastening through screw 41, make the bearing frame, thrust bearing and U type support piece relative position keep fixed. When the gear rotates, the bearing seat drives the first shaft to rotate relative to the U-shaped supporting piece through the thrust bearing 11.

In the existing low-temperature container, the centers of the bottoms of the inner container and the outer container are directly connected and fixed, and due to the large temperature difference between the inner container and the outer container, the outer part of the outer container is easy to dewfall through the connection mode. In the embodiment of the invention, the bottom of the inner container is fixedly connected with the inside of the outer tank through the heat insulation connecting piece 205 which is a nylon rope, the inner container can be tightly fixed on the inner wall of the outer tank through arranging 3-4 nylon ropes, the length of the connecting structure is lengthened, the transportation requirement is met, and meanwhile, the phenomena of heat leakage, frosting/dewing at the joint of the inner container and the outer container are effectively reduced.

Fig. 5 is a partial enlarged view of the object taking hole in fig. 1, as shown in fig. 5, a hollow cylindrical boss is installed at the object taking hole at the top of the outer tank 200, the inner wall of the boss is communicated with the object taking hole at the top of the inner tank, a groove 502 is formed at the edge of the boss outside the outer tank to reduce dew condensation, and a communication channel between the inner wall of the boss and the object taking hole at the top of the inner tank is of a bellows structure 501.

In the above embodiment of the present invention, the outer tank 200 includes the lower tank 201 and the upper end enclosure 202 hermetically connected to the tank, the outer side of the top of the end enclosure is a planar structure, and the top of the end enclosure 202 adopts a planar structure, so that an electrical box 207, a motor box for installing and fixing the driving assembly 400, a touch screen 208 for operation and control, and the like can be conveniently installed and fixed thereon. The inner side of the seal head is provided with a plurality of ribs 203 which can enhance the rigidity of the seal head and can fix pipelines in the container.

In the above embodiment of the present invention, foldable pedals 204 with different heights are further mounted at the lower part of the outer side of the outer tank 200, so that sampling personnel with different heights can conveniently take samples. The bottom of the outer tank 200 is provided with rollers 206 to facilitate the movement of the tank.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cryogenic storage device, comprising: the device comprises an outer tank, a shaft assembly, an inner container arranged in the outer tank and a driving assembly arranged outside the outer tank; the inner container is fixedly connected with the outer container, shaft holes for penetrating the shaft assemblies are respectively formed in the top of the outer container and the center of the top of the inner container, the shaft holes in the top of the outer container and the shaft holes in the top of the inner container are connected through corrugated pipes, and a first heat insulation assembly for blocking air flow convection is arranged between each corrugated pipe and each shaft assembly; an inner rotary body for placing biological samples in a partition mode is arranged on the shaft assembly positioned in the inner container, and the shaft assembly positioned outside the outer tank is connected with the driving assembly; the eccentric parts corresponding to the top of the inner container and the top of the outer tank are respectively provided with an object taking hole used as a biological sample taking and placing channel, and an openable heat preservation cover is arranged between the two object taking holes;

the shaft assembly includes: the first shaft and the second shaft are connected together through a second heat insulation assembly, the first shaft is connected with the driving assembly, and an inner rotating body is mounted on the second shaft;

the first shaft is of a multi-layer sleeve structure, the diameter of each layer of sleeve is different from the diameters of other layers of sleeves, adjacent layers of sleeves of the multi-layer sleeves are sequentially connected end to end, when the free end of the innermost layer of sleeve of the multi-layer sleeves is connected with the driving assembly, the free end of the outermost layer of sleeve is connected with the second shaft, or when the free end of the outermost layer of sleeve of the multi-layer sleeves is connected with the driving assembly, the free end of the innermost layer of sleeve is connected with the second shaft.

2. The apparatus of claim 1 wherein said first insulation assembly is an axially spaced fiberglass tube and a polytetrafluoroethylene spacer.

3. The apparatus of claim 1, wherein the second thermal insulation assembly comprises a first bushing and a second bushing connected by a first bolt, the first bushing is sleeved on one end of the first shaft and is connected and fixed with the first shaft by the first bolt, and the second bushing is sleeved on one end of the second shaft and is connected and fixed with the second shaft by the second bolt.

4. The apparatus of claim 3, wherein a third thermal insulation assembly is disposed between the first bolt and the second bushing and between the second bolt and the second bushing, respectively.

5. The device as claimed in claim 4, wherein the first sleeve is shaped as a boss, the second sleeve is fitted over the boss at the bottom thereof, and the first sleeve and the second sleeve are in line contact with each other.

6. The device of claim 1, wherein the multi-layer sleeve is a three-layer sleeve.

7. The device as claimed in any one of claims 1 to 6, wherein the output shaft of the drive assembly is provided with a first gear, the shaft assembly is provided with a bearing seat, and the bearing seat is provided with a second gear engaged with the first gear.

8. The apparatus of claim 7, wherein the axis of the first gear is perpendicular to the axis of the second gear.

9. The device according to any one of claims 1-6, wherein the bottom of the inner container is fixedly connected with the inside of the outer tank through a heat insulation connecting piece.

10. The apparatus of claim 9, wherein the insulated connector is a nylon rope.

11. The device as claimed in any one of claims 1 to 6, wherein a hollow cylindrical boss is mounted at the fetching hole on the top of the outer tank, the inner wall of the boss is communicated with the fetching hole on the top of the inner container, and a groove is formed on the edge of the boss positioned outside the outer tank.

12. The device as claimed in claim 11, wherein the communication channel between the inner wall of the boss and the fetching hole at the top of the inner container is of a bellows structure.

13. The device according to any one of claims 1-6, wherein the outer tank comprises a lower tank body and an upper seal head connected with the tank body in a sealing manner, the outer side of the top of the seal head is of a planar structure, and a plurality of ribs are arranged on the inner side of the seal head.