CN109609257A - The extracting method of Yi Zhong Channel-catfish fish guts oil - Google Patents
The extracting method of Yi Zhong Channel-catfish fish guts oil Download PDFInfo
- Publication number
- CN109609257A CN109609257A CN201811637503.4A CN201811637503A CN109609257A CN 109609257 A CN109609257 A CN 109609257A CN 201811637503 A CN201811637503 A CN 201811637503A CN 109609257 A CN109609257 A CN 109609257A
- Authority
- CN
- China
- Prior art keywords
- extracting method
- oil
- channel
- fish
- fish guts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B13/00—Recovery of fats, fatty oils or fatty acids from waste materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/74—Recovery of fats, fatty oils, fatty acids or other fatty substances, e.g. lanolin or waxes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides the extracting method of Yi Zhong Channel-catfish fish guts oil, the following steps are included: (1) feedstock processing: after channel catfish fish guts are removed gall-bladder, air bladder and enteral impurity, it is rinsed with water clean, then internal organ are smashed using tissue pulverizer, after homogenate, 4 DEG C of constant temperature are in store for；(2) above-mentioned cold storage raw material is thawed, carries out adding water process, is during which stirred continuously, in Xiang Shangshu aqueous mixture, suitable acid-base accommodation pH value is added；(3) in above-mentioned alkalescent system, papain 2500-3500U/g is added and digests 40min；(4) enzymolysis liquid is sent into supercentrifuge, isolates upper layer fish oil, obtains crude fish oil product.The channel catfishes Application of Viscera Oil recovery rate that extracting method provided by the invention is extracted is up to 90%；And method is easy to operate, extraction efficiency is high, is convenient for industrializing implementation.
Description
Technical field
The present invention relates to flavouring-soy-sauce technical fields, and in particular to the extracting method of Yi Zhong Channel-catfish fish guts oil.
Background technique
Currently, with the continuous improvement of China's fresh water fish crop and process scale, processing fent is also more and more, mainly
Including fish head, fish-bone, fish-skin, fish guts and its remaining meat mincing etc..These leftover bits and pieces remain in addition to being largely used as feed fish meal
Remaining part point arbitrarily can only be abandoned and be buried.
A large amount of fish guts can be generated in fish process, if being discarded, not only result in a large amount of wastes, it can also be to ring
It causes seriously to pollute in border.Therefore carrying out comprehensive processing and utilization to fish guts has important social and economic benefit.
The necessary a variety of fatty acid of 3 series fatty acid of ω and human body rich in fish oil, be widely used in drug and
Health care product.Just because of this, developed using fish guts manufacture fish oil, fish meal and its hydrolysate method, wherein have every
Water cooking process.Shanghai Aquatic Products Univ. 9CN) Yu Luli is to take Steam by water bath with the technique that the internal organ of grass carp, bream and silver carp extract fish oil
Cooking method.This method is that fish guts are cut into segment, is put into warmed-up temperature control water fluid bowl, and heating temperature is 80 DEG C, and the time is
70min carries out water proof boiling.During heating, primary every 5-10 minutes gentle agitations, when heated between half when be added it is fixed
Amount (1.0% fish guts weight) lye, to boiling after, mixed material is put into a centrifuge into centrifugal sedimentation 15min,
Centrifuge speed 3000-4000rpm separates oil mutually in container.
Summary of the invention
The purpose of the present invention is to provide the extracting method of Yi Zhong Channel-catfish fish guts oil, have that easy to operate, extraction efficiency is high,
The features such as convenient for industrializing implementation.
To achieve the above object, the present invention provides the extracting method of Yi Zhong Channel-catfish fish guts oil, comprising the following steps:
(1) it feedstock processing: after channel catfish fish guts are removed gall-bladder, air bladder and enteral impurity, is rinsed with water completely, so
Internal organ are smashed using tissue pulverizer afterwards, after homogenate, 4 DEG C of constant temperature are in store for；
(2) above-mentioned cold storage raw material is thawed, carries out adding water process, is during which stirred continuously, Xiang Shangshu aqueous mixture
In, suitable acid-base accommodation pH value is added；
(3) in above-mentioned alkalescent system, papain 2500-3500U/g is added and digests 40min；
(4) enzymolysis liquid is sent into supercentrifuge, isolates upper layer fish oil, obtains crude fish oil product.
Further, the liquid-to-solid ratio in the step (2) plus after water is 1mL:0.5-1.5g.
Further, the pH value in the step (2) plus after alkali is 7.0-7.6.
Further, the alkali is KOH.
Further, the reaction condition of step (3) enzymatic hydrolysis are as follows: entire enzymolysis process control reaction temperature is 40-60
DEG C, hybrid reaction objects system PH is constant.
Further, the revolving speed of step (4) centrifugation is 4500-5500r/min.
Further, step (4) centrifugation time is 5-15min.
The present invention has the advantages that
1,90% is up to using the channel catfishes Application of Viscera Oil recovery rate that this technique is extracted；
2, this method has the features such as easy to operate, extraction efficiency is high, is convenient for industrializing implementation；
3, raw material is processing of aquatic products by-product channel catfish fish guts, improves byproduct comprehensive utilization rate and adds
Value.
Detailed description of the invention
Fig. 1 is the oil extracting rate figure in embodiment 2 under different time.
Specific embodiment
The present invention will be further described with attached drawing With reference to embodiment, and following embodiment is intended to illustrate hair
Bright rather than limitation of the invention further.
Raw material of the present invention can synthesize commercially or according to the prior art.
Embodiment 1
The extracting method of Yi Zhong Channel-catfish fish guts oil, comprising the following steps:
(1) it feedstock processing: after channel catfish fish guts are removed gall-bladder, air bladder and enteral impurity, is rinsed with water completely, so
Internal organ are smashed using tissue pulverizer afterwards, after homogenate, 4 DEG C of constant temperature are in store for；
(2) above-mentioned cold storage raw material is thawed, carries out adding water process, the liquid-to-solid ratio after water is 1mL:0.5g, during which not
Disconnected to stir, in Xiang Shangshu aqueous mixture, it is 7.0 that suitable acid-base accommodation pH value, which is added,；
(3) in above-mentioned alkalescent system, papain 2500U/g is added and digests 40min；Entire enzymolysis process control
Reaction temperature is 40 DEG C, and hybrid reaction objects system PH is constant；
(4) enzymolysis liquid is sent into supercentrifuge, and the revolving speed of centrifugation is 4500r/min, and centrifugation time 5min is isolated
Layer fish oil, obtains crude fish oil product.
Embodiment 2:
The extracting method of Yi Zhong Channel-catfish fish guts oil, comprising the following steps:
(1) it feedstock processing: after channel catfish fish guts are removed gall-bladder, air bladder and enteral impurity, is rinsed with water completely, so
Internal organ are smashed using tissue pulverizer afterwards, after homogenate, 4 DEG C of constant temperature are in store for；
(2) above-mentioned cold storage raw material is thawed, carries out adding water process, the liquid-to-solid ratio after water is 1mL:1g, during which continuous
It stirs, in Xiang Shangshu aqueous mixture, it is 7.3 that suitable acid-base accommodation pH value, which is added,；
(3) in above-mentioned alkalescent system, papain 3000U/g is added and digests 40min；Entire enzymolysis process control
Reaction temperature is 50 DEG C, and hybrid reaction objects system PH is constant；
(4) enzymolysis liquid is sent into supercentrifuge, and the revolving speed of centrifugation is 5000r/min, centrifugation time 5-15min, separation
Upper layer fish oil out obtains crude fish oil product.
Embodiment 3
The extracting method of Yi Zhong Channel-catfish fish guts oil, comprising the following steps:
(1) it feedstock processing: after channel catfish fish guts are removed gall-bladder, air bladder and enteral impurity, is rinsed with water completely, so
Internal organ are smashed using tissue pulverizer afterwards, after homogenate, 4 DEG C of constant temperature are in store for；
(2) above-mentioned cold storage raw material is thawed, carries out adding water process, the liquid-to-solid ratio after water is 1mL:1.5g, during which not
Disconnected to stir, in Xiang Shangshu aqueous mixture, it is 7.6 that suitable acid-base accommodation pH value, which is added,；
(3) in above-mentioned alkalescent system, papain 3500U/g is added and digests 40min；Entire enzymolysis process control
Reaction temperature is 60 DEG C, and hybrid reaction objects system PH is constant；
(4) enzymolysis liquid is sent into supercentrifuge, and the revolving speed of centrifugation is 5500r/min, and centrifugation time 15min is isolated
Upper layer fish oil obtains crude fish oil product.
By taking specific embodiment 2 as an example, oil extracting rate test, obtained data such as the following table 1 are carried out, table 1 is every in 20-140min
The numerical value of oil extracting rate, and calculated mean value and standard deviation values are tested every 20min, Fig. 1 can be drawn out according to table 1；Table 2 is
Oil extracting rate numerical value under different temperatures, different enzyme concentrations, different time and different pH condition.
Table 1
Table 2
Utilize the secondary multinomial regression model between the Design-Expert oil extracting rate Y established and influence factor are as follows: Y=-
1184.19917+10.92250* temperature+0.029100* enzyme concentration -1.17675* time+259.57667*PH+1.48000*10-4* temperature * enzyme concentration+8.83333*10-3* temperature * time+0.14300* temperature * pH+5.41667*10-5* m- when enzyme concentration *
3.75000*10-3* enzyme concentration * pH+0.19183* time * pH-0.10608* temperature2-2.36333*10-6* enzyme concentration2-
6.15648*10-3* the time2-18.79833*pH2。
Above-mentioned numerical value is subjected to secondary multinomial regression model variance analysis, obtains following Table 3:
Table 3
It can be seen that model significance test is extremely significant (p < 0.01) by secondary multinomial regression model variance analysis, show
The model has statistical significance.Independent variable first order A (temperature) significant (P < 0.05), PH, time and significant (P < 0.01)；
Extremely significant (the P < 0.01) temperature of cross term time * PH, time, pH quadratic term are extremely significant (p < 0.01).Losing quasi- item P value is
0.6484 > 0.05 shows exist without losing pseudo-factor, thus can be used the regression equation replace the true point of test to experimental result into
Row analysis.
Regression coefficient R2(0.9613), coefficient of determination R is corrected2(0.9162) it is all larger than 0.80 and the coefficient of variation 1.69%, into
One step shows that models fitting goodness is preferable.
The above results show that the channel catfishes Application of Viscera Oil recovery rate extracted using this technique is up to 90%.
Finally, it should be noted that above embodiments are only to illustrate the present invention and not limit technology described in the invention
Scheme；Those skilled in the art should understand that still can modify to the present invention or equivalent replacement；And all are not
It is detached from the technical solution and its improvement of the spirit and scope of the present invention, should all be covered within the scope of the claims of the present invention.
Claims (7)
1. the extracting method of Yi Zhong Channel-catfish fish guts oil, which comprises the following steps:
(1) it feedstock processing: after channel catfish fish guts are removed gall-bladder, air bladder and enteral impurity, is rinsed with water completely, then adopts
Internal organ are smashed with tissue pulverizer, after homogenate, 4 DEG C of constant temperature are in store for；
(2) above-mentioned cold storage raw material is thawed, carries out adding water process, is during which stirred continuously, in Xiang Shangshu aqueous mixture, adds
Enter suitable acid-base accommodation pH value；
(3) in above-mentioned alkalescent system, papain 2500-3500U/g is added and digests 40min；
(4) enzymolysis liquid is sent into supercentrifuge, isolates upper layer fish oil, obtains crude fish oil product.
2. extracting method according to claim 1, which is characterized in that in the step (2) plus water after liquid-to-solid ratio be
1mL:0.5-1.5g。
3. extracting method according to claim 1, which is characterized in that the pH value in the step (2) plus after alkali is 7.0-
7.6。
4. extracting method according to claim 3, which is characterized in that the alkali is KOH.
5. extracting method according to claim 1, which is characterized in that the reaction condition of step (3) enzymatic hydrolysis are as follows: entire
It is 40-60 DEG C that enzymolysis process, which controls reaction temperature, and hybrid reaction objects system PH is constant.
6. extracting method according to claim 1, which is characterized in that the revolving speed of step (4) centrifugation is 4500-
5500r/min。
7. extracting method according to claim 1, which is characterized in that step (4) centrifugation time is 5-15min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811637503.4A CN109609257A (en) | 2018-12-29 | 2018-12-29 | The extracting method of Yi Zhong Channel-catfish fish guts oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811637503.4A CN109609257A (en) | 2018-12-29 | 2018-12-29 | The extracting method of Yi Zhong Channel-catfish fish guts oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109609257A true CN109609257A (en) | 2019-04-12 |
Family
ID=66015392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811637503.4A Pending CN109609257A (en) | 2018-12-29 | 2018-12-29 | The extracting method of Yi Zhong Channel-catfish fish guts oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109609257A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE8900206D0 (en) * | 1989-01-20 | 1989-01-20 | Felix Eriksson | PROCEDURE FOR EXTRACTION OF OIL FROM FISHING |
| US20030190392A1 (en) * | 2002-03-29 | 2003-10-09 | Seiichi Hiratsuka | Method for extracting lipid mixture containing phospholipids comprising polyunsaturated fatty acids from viscera of fish, method for preserving viscera prior to extraction, and lipid mixture extracted thereby |
| CN102942987A (en) * | 2012-11-08 | 2013-02-27 | 天津科技大学 | Method for extracting fish oil from head and internal organ of Scomber japonicus by using compound protease |
| CN103773590A (en) * | 2014-01-23 | 2014-05-07 | 吉首大学 | Method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease |
| CN105670764A (en) * | 2016-02-26 | 2016-06-15 | 陶建壮 | Method for extracting squid oil |
| CN105670765A (en) * | 2016-02-26 | 2016-06-15 | 陶建壮 | Method for extracting fish oil of tilapia wastes |
| CN105907456A (en) * | 2016-05-04 | 2016-08-31 | 浙江海洋大学 | Fine processing method of fish oil |
-
2018
- 2018-12-29 CN CN201811637503.4A patent/CN109609257A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE8900206D0 (en) * | 1989-01-20 | 1989-01-20 | Felix Eriksson | PROCEDURE FOR EXTRACTION OF OIL FROM FISHING |
| US20030190392A1 (en) * | 2002-03-29 | 2003-10-09 | Seiichi Hiratsuka | Method for extracting lipid mixture containing phospholipids comprising polyunsaturated fatty acids from viscera of fish, method for preserving viscera prior to extraction, and lipid mixture extracted thereby |
| CN102942987A (en) * | 2012-11-08 | 2013-02-27 | 天津科技大学 | Method for extracting fish oil from head and internal organ of Scomber japonicus by using compound protease |
| CN103773590A (en) * | 2014-01-23 | 2014-05-07 | 吉首大学 | Method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease |
| CN105670764A (en) * | 2016-02-26 | 2016-06-15 | 陶建壮 | Method for extracting squid oil |
| CN105670765A (en) * | 2016-02-26 | 2016-06-15 | 陶建壮 | Method for extracting fish oil of tilapia wastes |
| CN105907456A (en) * | 2016-05-04 | 2016-08-31 | 浙江海洋大学 | Fine processing method of fish oil |
Non-Patent Citations (2)
| Title |
|---|
| 王丹等: "酶解法提取鱿鱼内脏油的工艺研究及其脂肪酸分析", 《中国油脂》 * |
| 陆剑锋等: "水酶法提取斑点叉尾内脏油的工艺研究", 《食品科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102276716B (en) | Method for extracting unmodified natural collagen from bullfrog skin | |
| US11129400B2 (en) | Method of ecological utilization of silver carp | |
| CN105325801B (en) | Preparation method of sea cucumber intestinal peptide | |
| CN106047970B (en) | The extracting method of collagen polypeptide and calcium activated in wall pollack fish-bone | |
| CN103314912B (en) | Method of producing pearls on the basis of pearl shell mantel cell culture | |
| CN103030645B (en) | A kind of method of extensive preparation high-purity haemachrome and application | |
| CN104905256A (en) | Composite amino acid chelated calcium prepared by using oysters as raw materials | |
| CN102604734B (en) | Preparation method of zanthoxylum seed oil | |
| CN110787192B (en) | Application of soluble dietary fiber in kelp in preparing hypoglycemic drugs and functional foods | |
| CN103289812B (en) | Method for processing low-cholesterol and high-purity crab spawn oil | |
| CN107348122A (en) | A kind of raw fish tissue quality-improving special feed and its application | |
| CN103773590A (en) | Method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease | |
| CN104351848A (en) | Preparation method of crispy-bone pickled fish | |
| CN104026356A (en) | Pig feed for enhancing immunity and preparation method thereof | |
| CN108185176A (en) | With the feedstuff for live pigs for improving immune performance | |
| CN109609257A (en) | The extracting method of Yi Zhong Channel-catfish fish guts oil | |
| CN106387304A (en) | Method for preparing abalone viscera phospholipid through HPEF (High Pulsed Electric Field)-coupled biological enzymolysis | |
| CN104889141B (en) | Comprehensive utilization method for soybean dregs | |
| CN109486237A (en) | A kind of preparation method of ocean source melanin | |
| CN103304676A (en) | Method for preparing slowly-digestible starch through malic acid modification | |
| CN104642715A (en) | Method for extracting rice germ protein | |
| CN101999671A (en) | Application of silkworm pupa protein | |
| CN104223199A (en) | Shrimp ball processing process | |
| CN109527499A (en) | A kind of processing technology that ossein voluptuousness is remarkably reinforced | |
| CN108264576A (en) | The method for extracting water shield active material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190412 |
|
| RJ01 | Rejection of invention patent application after publication |