CN109609256A - A kind of method of improved extracting tea seed oil by using enzymatic hydrolysis - Google Patents
A kind of method of improved extracting tea seed oil by using enzymatic hydrolysis Download PDFInfo
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- CN109609256A CN109609256A CN201811532426.6A CN201811532426A CN109609256A CN 109609256 A CN109609256 A CN 109609256A CN 201811532426 A CN201811532426 A CN 201811532426A CN 109609256 A CN109609256 A CN 109609256A
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- enzymatic hydrolysis
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- oil
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/008—Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
Abstract
The invention discloses a kind of methods of improved extracting tea seed oil by using enzymatic hydrolysis, take tealeaves powder of seeds, under the rigid condition of 1:5-6 solid-liquid ratio, distilled water, 20-30% ethyl alcohol, 0.15-0.25mol/L sodium carbonate liquor are sequentially added into container, after mixing evenly by tea seeds slurries, it is 50-60 DEG C that temperature, which is arranged, adds 500-700U/g alkali protease；The enzymolysis process of 3-4h is carried out, uses agitating paddle in enzymolysis process, revolving speed 70-80r/min is stirred；After the completion of enzymatic hydrolysis, tea seed slurry liquid is centrifuged, revolving speed 3200-5000r/min, is centrifuged 25-35min, isolates edible vegetable oil and emulsion layer；Suitable dehydrated alcohol is added in emulsion layer, is centrifuged again after mixing evenly, 3200-5000r/min, 25-35min take out edible vegetable oil.Edible vegetable oil yield of the present invention and oily yield are high.
Description
Technical field
The present invention relates to a kind of methods of improved extracting tea seed oil by using enzymatic hydrolysis.
Background technique
In recent years, aqueous enzymatic method is as a kind of relatively advanced oil extracting process, have oil yield is high, quality of crude oil is good, lighter color,
The advantages such as production energy consumption is low, green, the safe and efficient idea of development for meeting clean manufacturing and comprehensive utilization.With aqueous enzymatic method skill
Extensive research of the art in a variety of oil crops greases such as yellow meal worm, peanut, walnut, grape pip, olive extract, it was demonstrated that with tradition
Oil extracting process compare, aqueous enzymatic extraction condition is milder, low operation temperature, and the oil quality of extraction is preferably etc. excellent
Point, production process pollution is less and degradation product does not react generally with extract, can be very good nutrition in protection grease
Substance, especially trace active ingredient, for can the substance of comprehensive development and utilization have good protective effect.
Aqueous enzymatic method is when extracting tea seed oil, using tea seed is mechanically pulverized, is then cut grease using enzyme big with other
The complex that molecule combines decomposes lipoprotein by enzyme, discharges grease, increases the mobility of grease, Lai Tigao tea seeds
Oil yield.But during existing extracting tea seed oil by using enzymatic hydrolysis, there are grease easily emulsify cause edible vegetable oil rate it is low, to be difficult into
The problem of row industrialized production.
Summary of the invention
The main object of the present invention is to provide a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis.
The used technical solution that the present invention solves its technical problem is:
Technical solution one:
A kind of method of improved extracting tea seed oil by using enzymatic hydrolysis, includes the following steps:
Take tealeaves powder of seeds, under the rigid condition of 1:5-6 solid-liquid ratio, sequentially add distilled water, 20-30% ethyl alcohol,
0.15-0.25mol/L sodium carbonate liquor,；The temperature that recirculated water bath is arranged is 50-60 DEG C, and tea seeds slurries are poured into container
In, after mixing evenly, the enzymolysis process that 500-700U/g alkali protease carries out 3-4h is added, uses stirring in enzymolysis process
Paddle, revolving speed 70-80r/min are carried out；After the completion of enzymatic hydrolysis, tea seed slurry liquid is centrifuged, revolving speed 3200-3800r/min, is centrifuged 25-
35min；Aqueous extract and boiled oil layer, emulsion layer are poured into separatory funnel together, 1~2h is stood, isolates edible vegetable oil and emulsification
Layer, is weighed；Suitable dehydrated alcohol is added in emulsion layer, pours into centrifuge tube after mixing evenly, is centrifuged again, 3200-
3800r/min, 25-35min take out edible vegetable oil.
Preferably, the method for a kind of improved extracting tea seed oil by using enzymatic hydrolysis, includes the following steps:
Take tealeaves powder of seeds, under the rigid condition of 1:5.5 solid-liquid ratio, sequentially add distilled water, 25% ethyl alcohol,
0.2mol/L sodium carbonate liquor.The temperature that recirculated water bath is arranged is 55 DEG C, and tea seeds slurries are poured into container, are stirred evenly
Afterwards, the enzymolysis process that 600U/g alkali protease carries out 3.5h is added, uses four leaf agitating paddles, revolving speed 75r/ in enzymolysis process
Min is carried out；After the completion of enzymatic hydrolysis, tea seed slurry liquid is centrifuged, revolving speed 3500r/min, is centrifuged 30min；By aqueous extract
And boiled oil layer, emulsion layer pour into separatory funnel together, stand 1~2h, isolate edible vegetable oil and emulsion layer, weighed；Emulsion layer
It is middle that suitable dehydrated alcohol is added, it pours into centrifuge tube, is centrifuged again after mixing evenly, 3500r/min, 30min take out clear
Oil.
Another technical solution of the invention are as follows:
A kind of method of improved extracting tea seed oil by using enzymatic hydrolysis, includes the following steps:
Take tealeaves powder of seeds, under the rigid condition of 1:5-6 solid-liquid ratio, sequentially add distilled water, 20-30% ethyl alcohol,
0.15-0.25mol/L sodium carbonate liquor；The temperature that recirculated water bath is arranged is 50-60 DEG C, and tea seeds slurries are poured into container
In, after mixing evenly, the enzymolysis process that 500-700U/g alkali protease carries out 3-4h is added, uses stirring in enzymolysis process
Paddle, revolving speed 70-80r/min are carried out；After the completion of enzymatic hydrolysis, tea seed slurry liquid is poured into plate and frame filter press and is separated by solid-liquid separation, by water
Oily to be mutually centrifuged, revolving speed 3200-3800r/min is centrifuged 25-35min；Edible vegetable oil and emulsion layer are isolated, is weighed；In emulsion layer
Suitable dehydrated alcohol is added, pours into centrifuge tube after mixing evenly, is centrifuged again, 3200-3800r/min, 25-35min take
Edible vegetable oil out.
Preferably, the method for a kind of improved extracting tea seed oil by using enzymatic hydrolysis, includes the following steps:
Take tealeaves powder of seeds, under the rigid condition of 1:5.5 solid-liquid ratio, sequentially add distilled water, 25% ethyl alcohol,
0.2mol/L sodium carbonate liquor；The temperature that recirculated water bath is arranged is 55 DEG C, and tea seeds slurries are poured into container, are stirred evenly
Afterwards, the enzymolysis process that 600U/g alkali protease carries out 3.5h is added, uses four leaf agitating paddles, revolving speed 75r/ in enzymolysis process
Min is carried out；After the completion of enzymatic hydrolysis, tea seed slurry liquid is poured into plate and frame filter press and is separated by solid-liquid separation, water oil is mutually centrifuged, is turned
Fast 3500r/min is centrifuged 30min；Edible vegetable oil and emulsion layer are isolated, is weighed；Suitable dehydrated alcohol is added in emulsion layer,
It pours into centrifuge tube, is centrifuged again after mixing evenly, 3500r/min, 30min take out edible vegetable oil.
Preferably, the tealeaves powder of seeds crosses 60 meshes.
Preferably, suitable dehydrated alcohol is added in emulsion layer, for 5-15% volume ratio ethyl alcohol is added.
Further preferably, suitable dehydrated alcohol is added in emulsion layer, for 15% volume ratio ethyl alcohol is added.
The technical program compared with the background art, it has the following advantages:
Aqueous enzymatic method as efficient and safe oil extracting methods, the technical process the problem of mainly because of protein and
Emulsion caused by phosphatide etc., elimination and demulsification to emulsion are that extracting tea seed oil by using enzymatic hydrolysis production technology needs to dash forward
Broken problem.General demulsification means carry out all after oil extracting process, increase the operating procedure of technique and can not break completely
Cream, common demulsification means have heating, freeze-thaw, enzyme process, pH method etc..The present invention assists aqueous enzymatic extraction tealeaves using ethyl alcohol
Seed oil, reduces the generation of emulsion during oil extracting process, improves edible vegetable oil amount, simplifies process flow.The present invention is adding
On the basis of entering ethyl alcohol elimination emulsion, alkaline reaction system is provided for alkali protease using sodium carbonate, is passed through introducing salt
Effect carries out process optimization, efficiently solves the problems, such as demulsification, and the dosage of salt is greatly reduced, and ethyl alcohol revolving recycling is repeatable
It utilizes.
(1) under the conditions of alkali protease aqueous enzymatic extraction technique optimized parameter, the Tea Saponin of its aqueous extract is measured
Content, measuring Tea Saponin yield in waste liquid and waste residue is respectively 2.12% and 3.76%, and it is comprehensive to show that aqueous enzymatic extraction technique has
Run the advantage of hair jointly.
(2) amplify scale, be 55 DEG C in reaction temperature, enzyme concentration is 600U/g alkali protease, and 25% ethyl alcohol substitution is steamed
Distilled water, 0.2mol/L sodium carbonate liquor, solid-liquid ratio 1:5.5, enzyme digestion reaction 3.5h use four leaf agitating paddles, revolving speed 75r/min
Be separated by solid-liquid separation under enzymatic hydrolysis condition using plate and frame filter press, acquisition highest oil yield and edible vegetable oil rate, respectively 19.2%
With 94.5%.
Specific embodiment
In following all examples, tea seeds come from Suizhou, hubei Yunwu Mountain, after tea seeds dry, use cutting and grinding
Machine crushes tea seeds, with 60 mesh sieves.Tealeaves powder of seeds is poured into batches in pot and is fried to yellowish-brown, every batch of keeps matter
Amount is consistent, after 60 meshes.
Embodiment 1
Using 3Kg tealeaves powder of seeds as scale-up dosage, under the rigid condition of 1:5.5 solid-liquid ratio, steaming is sequentially added
Distilled water, 25% ethyl alcohol, 0.2mol/L sodium carbonate liquor.The temperature that recirculated water bath is arranged is 55 DEG C, and tea seeds slurries are poured into
In fermentor, after mixing evenly, the enzymolysis process that 600U/g (9g) alkali protease carries out 3.5h is added, is used in enzymolysis process
Four leaf agitating paddles, revolving speed 75r/min are carried out.After the completion of enzymatic hydrolysis, tea seed slurry liquid is poured into 750mL tophan box and is centrifuged,
Revolving speed 3500r/min is centrifuged 30min；Aqueous extract and boiled oil layer, emulsion layer are poured into separatory funnel together, stand 1~
2h isolates edible vegetable oil and emulsion layer, is weighed；It is added in emulsion layer suitable dehydrated alcohol (volume ratio 15%), stirring is equal
It pours into centrifuge tube after even, is centrifuged again, 3500r/min, 30min take out edible vegetable oil, weigh and calculate.According to formula 2-1,2-
2,2-3 is calculated.
M=m1+m2 (2-1)
Wherein: m --- total oil sample quality (g) of extraction
m1--- the quality (g) of edible vegetable oil
m2--- the oil sample quality (g) obtained after emulsion layer demulsification
M --- tea seed silty amount (g)
The oily yield and edible vegetable oil accounting of acquisition, respectively 19.0% and 94.2%
Embodiment 2
Using 3Kg tealeaves powder of seeds as scale-up dosage, under the rigid condition of 1:5.5 solid-liquid ratio, steaming is sequentially added
Distilled water, 25% ethyl alcohol, 0.2mol/L sodium carbonate liquor.The temperature that recirculated water bath is arranged is 55 DEG C, and tea seeds slurries are poured into
In fermentor, after mixing evenly, the enzymolysis process that 600U/g (9g) alkali protease carries out 3.5h is added, is used in enzymolysis process
Four leaf agitating paddles, revolving speed 75r/min are carried out.After the completion of enzymatic hydrolysis, tea seed slurry liquid is poured into plate and frame filter press and is separated by solid-liquid separation,
Water oil is mutually poured into tophan box and is centrifuged, revolving speed 3500r/min is centrifuged 30min；Edible vegetable oil and emulsion layer are isolated, is carried out
It weighs；Suitable dehydrated alcohol (volume ratio 15%) is added in emulsion layer, pours into centrifuge tube, is centrifuged again after mixing evenly,
3500r/min, 30min take out edible vegetable oil, weigh and calculate.It is calculated according to formula 2-1,2-2,2-3.
Acquisition highest oil yield and edible vegetable oil accounting, respectively 19.2% and 94.5%.
The present embodiment is compared with embodiment 1, after the completion of enzymatic hydrolysis, using plate and frame filter press be separated by solid-liquid separation instead of using
The step of centrifuge separates, the case where comparing tea seed oil yield and edible vegetable oil amount.In the present embodiment, sheet frame is used after the completion of enzymatic hydrolysis
Filter press is separated by solid-liquid separation, and the separating effect compared to centrifuge will more sufficiently can be good at leached tea oil slag and grease phase
It separates, to improve oily yield, oily yield is 19.2%, and edible vegetable oil accounting is 94.5%.As it can be seen that plate and frame filter press is as work
Common equipment in industry production has well adapting to property for technique.Compared to centrifuge as needed for instrument capacity limitation
The longer feature of duration, plate and frame filter press greatly reduce the length of test period, and separation completely, while can also reduce energy
Consumption.
Under the conditions of alkali protease aqueous enzymatic extraction technique optimized parameter, the Tea Saponin for measuring its aqueous extract contains
Amount, measuring Tea Saponin yield in waste liquid and waste residue is respectively 2.12% and 3.76%, shows that aqueous enzymatic extraction technique has synthesis
The advantage of exploitation.
Comparative example 1
It is tested with 2000g tea seed powder, setting ethanol concentration gradient is 20%, 25%, 30%, 35%, 40%.Equally
Under the conditions of solid-liquid ratio is 1:5.5,600U/g alkali protease, pH=9.3, addition respective concentration ethyl alcohol, 55 DEG C, 55r/min
It carries out digesting for 3.5 hours under revolving speed.
As shown in table 1, the highest oily yield of ethyl alcohol auxiliary extracting tea seed oil by using enzymatic hydrolysis and edible vegetable oil measure present addition
When 25% ethyl alcohol, oily yield is 18.4%, and edible vegetable oil accounting reaches 89.8%.But when continuing to improve concentration of alcohol, the oil of system
Yield and edible vegetable oil amount decline instead.It is known that can be obtained using ethyl alcohol auxiliary extracting tea seed oil by using enzymatic hydrolysis from result
Optimal oily yield is obtained, the generation of alleviation emulsion is significantly to improve edible vegetable oil amount.
Influence of 1 concentration of alcohol of table to system emulsion
Comparative example 2
Using 2000g tea seeds powder as test dose, solid-liquid ratio 1:5.5,25% ethyl alcohol, addition 600U/g alkali protease
Under the conditions of, it is digested in various concentration gradient sodium carbonate liquor.As shown in table 2, concentration of alcohol under the same conditions, be added
The sodium carbonate liquor of 0.2mol/L concentration can get highest oily yield 18.8%, wherein boiled oil layer accounting 94.1%, compared to not
The oily yield 18.4% and edible vegetable oil accounting 89.8% of system, are all significantly improved when salting liquid is added.In addition, introducing
0.2mol/L sodium carbonate liquor can save and adjust pH with sodium hydroxide solution, be beneficial to grease safety and quality.Grease simultaneously
During extraction, sodium carbonate liquor can also carry out alkali refining processing to grease, remove free fatty acid, improve oil quality.Most
Importantly, introducing salt effect combination ethyl alcohol assists extracting tea seed oil by using enzymatic hydrolysis, the formation of water phase extracting and emulsifying liquid is reduced, and
It is not to be demulsified again after forming lotion, achievees the purpose that improve edible vegetable oil rate.
The optimization of 2 concentration of salt solution of table
Comparative example 3
It is essentially identical with embodiment 1, the difference is that being applied to tea seed oil aqueous enzymatic extraction work using neutral proteinase
Skill, emulsion is even more serious than alkali protease, compares generally speaking, neutral proteinase to the extraction effect of tea seed oil not
Such as alkali protease, oily yield and edible vegetable oil yield are all substantially reduced.Highest oil yield is 17.6%, edible vegetable oil accounting 81.8%.
Comparative example 4
With 2000g tea seeds, under the conditions of solid-liquid ratio 1:5.5,600U/g alkali protease, pH=9.3, it is added without ethyl alcohol and mentions
Emulsion layer is collected after oil, using 50g emulsion layer as test dose.As shown in table 3, using the dehydrated alcohol of 15% volume ratio to cream
Change layer to be demulsified to obtain highest oil yield 59.8%, the demulsification of ethyl alcohol is best at this time.When dehydrated alcohol volume ratio 5%~
When 15%, with the rising of concentration of alcohol, demulsification is increased accordingly, and when concentration of alcohol is greater than 15%, demulsification is gradually
Decline.
The effect that 3 ethyl alcohol of table is demulsified to emulsion layer
The above is only the preferred embodiment of the present invention, the range implemented of the present invention that therefore, it cannot be limited according to, i.e., according to
Equivalent changes and modifications made by the invention patent range and description, should still be within the scope of the present invention.
Claims (10)
1. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis, includes the following steps:
Tealeaves powder of seeds is taken, under the rigid condition of 1:5-6 solid-liquid ratio, sequentially adds distilled water, 20-30% ethyl alcohol, 0.15-
0.25mol/L sodium carbonate liquor；The temperature that recirculated water bath is arranged is 50-60 DEG C, and tea seeds slurries are poured into container and are stirred
After uniformly, 500-700U/g alkali protease is added, the enzymolysis process of 3-4h is carried out, uses agitating paddle in enzymolysis process, turn
Fast 70-80r/min is carried out；After the completion of enzymatic hydrolysis, tea seed slurry liquid is centrifuged, revolving speed 3200-5000r/min, is centrifuged 25-35min；
Aqueous extract and boiled oil layer, emulsion layer are poured into separatory funnel together, 1~2h is stood, isolates edible vegetable oil and emulsion layer, is carried out
It weighs；Suitable dehydrated alcohol is added in emulsion layer, pours into centrifuge tube after mixing evenly, is centrifuged again, 3200-3800r/
Min, 25-35min take out edible vegetable oil.
2. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis, includes the following steps:
Tealeaves powder of seeds is taken, under the rigid condition of 1:5.5 solid-liquid ratio, sequentially adds distilled water, 25% ethyl alcohol, 0.2mol/L carbon
Acid sodium solution；The temperature that recirculated water bath is arranged is 55 DEG C, and tea seeds slurries are poured into container, after mixing evenly, are added
600U/g alkali protease carries out the enzymolysis process of 3.5h, four leaf agitating paddles is used in enzymolysis process, and revolving speed 75r/min is carried out；
After the completion of enzymatic hydrolysis, tea seed slurry liquid is centrifuged, revolving speed 3500r/min, is centrifuged 30min；By aqueous extract and boiled oil layer, emulsification
Layer pours into separatory funnel together, stands 1~2h, isolates edible vegetable oil and emulsion layer, weighed；Suitable nothing is added in emulsion layer
Water-ethanol pours into centrifuge tube after mixing evenly, is centrifuged again, 3500r/min, 30min, takes out edible vegetable oil.
3. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis according to claim 1 or 2, it is characterised in that: institute
The tealeaves powder of seeds stated crosses 60 meshes.
4. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis according to claim 1, it is characterised in that: emulsion layer
It is middle that suitable dehydrated alcohol is added, for 5-15% volume ratio ethyl alcohol is added.
5. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis according to claim 2, it is characterised in that: emulsion layer
It is middle that suitable dehydrated alcohol is added, for 15% volume ratio ethyl alcohol is added.
6. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis, includes the following steps:
Tealeaves powder of seeds is taken, under the rigid condition of 1:5-6 solid-liquid ratio, sequentially adds distilled water, 20-30% ethyl alcohol, 0.15-
0.25mol/L sodium carbonate liquor,；The temperature that recirculated water bath is arranged is 50-60 DEG C, and tea seeds slurries are poured into container, are stirred
After mixing uniformly, 500-700U/g alkali protease is added, carries out the enzymolysis process of 3-4h, uses agitating paddle in enzymolysis process, turn
Fast 70-80r/min is carried out；After the completion of enzymatic hydrolysis, tea seed slurry liquid is poured into plate and frame filter press and is separated by solid-liquid separation, by water oil mutually into
Row centrifugation, revolving speed 3200-3800r/min are centrifuged 25-35min；Edible vegetable oil and emulsion layer are isolated, is weighed；Add in emulsion layer
Enter suitable dehydrated alcohol, pour into centrifuge tube after mixing evenly, be centrifuged again, 3200-3800r/min, 25-35min take out
Edible vegetable oil.
7. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis according to claim 1, includes the following steps:
Tealeaves powder of seeds is taken, under the rigid condition of 1:5.5 solid-liquid ratio, sequentially adds distilled water, 25% ethyl alcohol, 0.2mol/L carbon
Acid sodium solution；The temperature that recirculated water bath is arranged is 55 DEG C, and tea seeds slurries are poured into container, after mixing evenly, is added
600U/g alkali protease carries out the enzymolysis process of 3.5h, and four leaf agitating paddles are used in enzymolysis process, and revolving speed 75r/min is carried out；
After the completion of enzymatic hydrolysis, tea seed slurry liquid is poured into plate and frame filter press and is separated by solid-liquid separation, water oil is mutually centrifuged, revolving speed 3500r/min,
It is centrifuged 30min；Edible vegetable oil and emulsion layer are isolated, is weighed；Suitable dehydrated alcohol is added in emulsion layer, falls after mixing evenly
Enter in centrifuge tube, be centrifuged again, 3500r/min, 30min take out edible vegetable oil.
8. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis according to claim 6 or 7, it is characterised in that: institute
The tealeaves powder of seeds stated crosses 60 meshes.
9. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis according to claim 6, it is characterised in that: emulsion layer
It is middle that suitable dehydrated alcohol is added, for 5-15% volume ratio ethyl alcohol is added.
10. a kind of method of improved extracting tea seed oil by using enzymatic hydrolysis according to claim 7, it is characterised in that: emulsification
Suitable dehydrated alcohol is added in layer, for 15% volume ratio ethyl alcohol is added.
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| CN104450152A (en) * | 2013-09-23 | 2015-03-25 | 贵州周以晴生物科技有限公司 | Compound technology for extracting tea seed oil employing aqueous enzymatic method |
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| CN105623833A (en) * | 2015-09-16 | 2016-06-01 | 华侨大学 | Demulsification treatment method for aqueous enzymatic method extracted tea seed oil |
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- 2018-12-14 CN CN201811532426.6A patent/CN109609256A/en active Pending
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| CN104450152A (en) * | 2013-09-23 | 2015-03-25 | 贵州周以晴生物科技有限公司 | Compound technology for extracting tea seed oil employing aqueous enzymatic method |
| CN104435363A (en) * | 2013-09-23 | 2015-03-25 | 贵州周以晴生物科技有限公司 | Method for extracting polyphenol from tea seeds by adopting aqueous enzymatic process-ethanol reflux method |
| CN104928012A (en) * | 2015-05-26 | 2015-09-23 | 南昌大学 | Method for extracting tea oil by demulsification based on aqueous enzymatic method |
| CN104974847A (en) * | 2015-06-09 | 2015-10-14 | 武汉轻工大学 | Aqueous enzymatic method for simultaneous preparation of camellia seed oil and camellia seed protein |
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