Background
Generally, when some animal gas concentration model experiments are carried out, an animal raising box is used for isolating experimental animals from the external environment, and the gas model experiments of the animals are carried out through controlling the oxygen concentration. Since such rearing tanks are generally relatively closed and have a low gas exchange capacity, the CO2 gas produced by the metabolism of animals cannot be sufficiently discharged. The gas can be accumulated continuously, the concentration is increased continuously, the health of experimental animals is influenced, and even acidosis of the experimental animals is caused. The following table shows the relationship between the concentration and content of CO2 and the physiological response of the organism.
CO2Concentration of
|
Physiological response
|
350~450ppm
|
The same general outdoor environment
|
350~1000ppm
|
Fresh air and smooth breath
|
>10,00ppm
|
The air was turbid and the drowsiness began to be felt
|
>20,00ppm
|
Headache, lethargy, dull, inattentive, accelerated heartbeat, mild nausea
|
>50,00ppm
|
Can lead to severe hypoxia, permanent brain damage, coma, and even death |
At present, CO reduction2The concentration method mainly comprises the following two methods:
1. gas dilution, i.e. by injection of N2To dilute CO in a specific space2And through O2The control of the concentration reaches a target value. The main disadvantages of this approach are:
due to CO2The limit value of (A) is in the ppm level, a large amount of N needs to be injected2And O2To reduce CO2The concentration of (2) is high, the cost is high, and the efficiency is low.
2. Directly putting a large amount of adsorbent for adsorption. The disadvantages of this approach are:
the utilization rate of the adsorbent is not high, which causes waste.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides CO in a closed environment2The control device improves the utilization rate of the adsorbent and reduces the cost.
The purpose of the invention is realized by the following technical scheme:
CO in closed environment2The control device comprises a closed feeding box; CO in the closed environment2The control device of (1) further comprising:
a plurality of chambers disposed at the top of the feeding box; a switch is arranged on one side of the cavity adjacent to the interior of the feeding box and used for controlling the communication between the interior of the cavity and the interior of the feeding box, and CO is suitable to be placed in the cavity2An adsorbent;
a sensor for detecting CO in the rearing chamber2Content A1、CO2Generation rate of (A)2And O2And transmitting to a processor;
a processor, the processor based on the received A1、A2And B gives the area S:
when A is
1When the carbon content is larger than the carbon content,
when A is
1When the content is less than or equal to C, k is 0; d is CO in the breeding box
2And C is (0, D);
the detection unit is used for detecting the position of the animal in the feeding box and transmitting the control unit;
a control unit that adjusts the switch such that the sum of the areas of the openings at the bottom of the chamber above the location matches the area S.
The invention also aims to provide CO in the closed environment based on the control device2The purpose of the invention is realized by the following technical scheme:
CO in a closed environment according to the above-mentioned control device2The control method of (2), said control method comprising the steps of:
(A1) sensor detects CO in raising case2Content A1、CO2Generation rate of (A)2And O2And transmitting the content B to a processor, wherein the processor outputs an area S;
the detection unit detects the position of the animal in the feeding box and transmits the control unit;
(A2) the control unit adjusts the switch to enable the sum of the areas of the openings at the bottom of the chamber above the position to be matched with the area S;
(A3) the adsorbent in the opened chamber adsorbs CO in the feeding box2Thereby introducing CO into the rearing chamber2The content of (a) is reduced to a safe level.
Compared with the prior art, the invention has the beneficial effects that:
1. accurate control is realized: determining the area of the chamber to be opened (adsorbent adsorption) according to the parameters of the gas in the feeding chamber, and detecting the position of the animal (CO at the position)2High content) to open the chamber above the animal's locus to expose the adsorbent and thereby reduce CO at the animal's locus2In an amount such that it is at a safe level;
2. without using N2Remarkably reduce the costThen, the process is carried out;
3. the capacity prediction of the experimental animals in the feeding boxes with different volumes is realized.
Detailed Description
Fig. 1 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. Some conventional aspects have been simplified or omitted for the purpose of teaching the present invention. Those skilled in the art will appreciate that variations or substitutions from these embodiments will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
FIG. 1 is a schematic diagram showing CO in an enclosed environment according to example 1 of the present invention2The control device of (2) is schematically shown in FIG. 1, and CO is in the closed environment2The control device of (1) comprises:
the breeding box belongs to the prior art in the field, and the specific structure and the working mode are not described again;
a plurality of chambers disposed at the top of the feeding box; a switch is arranged on one side of the cavity adjacent to the interior of the feeding box and used for controlling the communication between the interior of the cavity and the interior of the feeding box, and CO is suitable to be placed in the cavity2An adsorbent;
a sensor for detecting CO in the rearing chamber2Content A1、CO2Generation rate of (A)2And O2And transmitting to a processor;
a processor, the processor based on the received A1、A2And B gives the area S:
when A is
1When the carbon content is larger than the carbon content,
when A is
1When the content is less than or equal to C, k is 0; d is CO in the breeding box
2And C is (0, D); the C value is determined artificially, and CO in the breeding box is expected
2The lower the content of (A), the lower the C value is set, but generally not lower than the CO content in the air
2The content of (a).
The detection unit is used for detecting the position of the animal in the feeding box and transmitting the control unit;
a control unit that adjusts the switch such that the sum of the areas of the openings at the bottom of the chamber above the location matches the area S.
For the sake of uniformity, further, the chambers are arranged on the top of the breeding box in a grid shape.
In order to obtain the position of the animal in the feeding chamber in order to open the chamber above the animal accurately, further, the detecting unit employs a gravity sensing piece or a camera.
To automatically open the chamber to allow the adsorbent to adsorb CO in the feeding chamber2Further, the switch includes:
a door rotatably fixed to a bottom of the chamber;
an electronic lock for controlling whether the door and the chamber are connected or not.
CO in closed Environment of embodiments of the invention2The control method of (2), that is, the working process of the control device, the control method comprises the following steps:
(A1) sensor detects CO in raising case2Content A1、CO2Generation rate of (A)2And O2And transmitting the content B to a processor, wherein the processor outputs an area S;
the detection unit detects the position of the animal in the feeding box and transmits the control unit;
(A2) the control unit adjusts the switch to enable the sum of the areas of the openings at the bottom of the chamber above the position to be matched with the area S; matching does not mean that the sum of the areas and the area S must be equal, in view of the sum of the areas
The discreteness of (A) cannot be achieved by S
nIs equal to S, but requires S
n≤S<S
n+1;
(A3) The adsorbent in the opened chamber adsorbs CO in the feeding box2Thereby introducing CO into the rearing chamber2The content of (a) is reduced to a safe level.
In order to conveniently open the chamber, further, the switch is adjusted in a manner that:
when the control unit outputs an opening instruction, the electronic lock is opened according to the instruction, and the sealing door at the bottom of the cavity is separated from the cavity and descends under the action of gravity, so that the interior of the cavity is communicated with the interior of the feeding box.
Example 2:
CO in closed Environment according to example 1 of the invention2An application example of the control device and the method of (1).
In the application example, the opening area of each chamber is equal, and a plurality of chambers are arranged on the top of the breeding box in a grid manner; the switch adopts a door structure, the door is rotatably fixed at the bottom of the chamber, and a sealing strip is arranged between the door and the chamber; the door and the chamber are connected through the electronic lock, but when the electronic lock is opened, the door and the chamber are separated and descend under the action of gravity; the detection unit adopts a gravity sensing piece to sense the position of the animal in the feeding box.
Using animal raising box, the experimental condition is to put O in the box2Concentration was maintained at 10% in the experimentThe mice were 40 BALB/C mice. Mice in hypoxic environments appear to be quiet, immotile, and clump together.
800ppm of C and 1200ppm of D; the gravity sensing piece senses the mice close to each other, CO2Rate of rise A2At 800ppm/min, after treatment, open the area above the mouse 2280cm2Exposing the adsorbent therein.