CN109602011A - A kind of modified silkworm chrysalis grease and preparation method thereof - Google Patents
A kind of modified silkworm chrysalis grease and preparation method thereof Download PDFInfo
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- CN109602011A CN109602011A CN201910123006.0A CN201910123006A CN109602011A CN 109602011 A CN109602011 A CN 109602011A CN 201910123006 A CN201910123006 A CN 201910123006A CN 109602011 A CN109602011 A CN 109602011A
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- silkworm chrysalis
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- lipase
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 89
- 239000004519 grease Substances 0.000 title claims abstract description 78
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
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- 238000011049 filling Methods 0.000 description 6
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 6
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- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 5
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- 235000013336 milk Nutrition 0.000 description 3
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- 150000002170 ethers Chemical class 0.000 description 1
- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 235000021472 generally recognized as safe Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
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- FCCDDURTIIUXBY-UHFFFAOYSA-N lipoamide Chemical compound NC(=O)CCCCC1CCSS1 FCCDDURTIIUXBY-UHFFFAOYSA-N 0.000 description 1
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- 230000007935 neutral effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- -1 phosphatide Substances 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
- A23D9/013—Other fatty acid esters, e.g. phosphatides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention discloses a kind of modified silkworm chrysalis greases and preparation method thereof, include the following steps: (1) using dry silkworm chrysalis as raw material, solvent extraction is utilized after crushing, obtains the extract liquor of grease containing silkworm chrysalis and solvent, and moisture content is not higher than 0.5% in extract liquor；(2) immobilized lipase is added in extract liquor, constantly addition ethyl alcohol, control reaction system moisture content is not higher than 2%, under agitation, carries out alcoholysis reaction；(3) lipase is separated and recovered, obtained oil is mutually carried out molecular distillation by recycling design, except the fatty-acid ethyl ester that dereaction generates, obtains modified silkworm chrysalis grease.The present invention improves the hypolipemic function and oxidation stability of silkworm chrysalis grease using the modified silkworm chrysalis grease of fixed lipase catalyzed preparation.This method is not needed silkworm chrysalis oil refinement, while other active materials such as remain phosphatide, and simplifies technical process, has better economy and the feature of environmental protection.
Description
Technical field
The present invention relates to a kind of silkworm chrysalis Grease preparation method for strengthening auxiliary lipid-lowering function and applications.
Background technique
Silkworm chrysalis grease has the function of auxiliary reducing blood lipid, and patent CN108094551A develops a kind of energy using silkworm chrysalis grease
Milk tablet is made in the silkworm chrysalis oil milk tablet for enough reducing blood lipid, the silkworm chrysalis oil by adding 2-10% in milk powder, which can reduce
Blood lipid, cholesterol improve liver function.Main component in silkworm chrysalis grease with auxiliary lipid-lowering function is alpha-linolenic acid (ALA)
Glyceride, phosphatide and liposoluble vitamin and sterol substance.Alpha-linolenic acid (ALA) belongs to n-3 series polyunsaturated fatty acid,
It is the precursor of synthesis EPA, DHA in one of essential fatty acid and human body.Glyceride and phosphatide rich in ALA are to human body
A variety of diseases especially to adjust blood lipid, reduce blood pressure, antitumor, prevention cardiovascular and cerebrovascular disease etc. has special curative effect.Closely
Phase is the study found that the diglyceride containing ALA has more efficient reducing blood lipid, weight losing function.Diglyceride (diglyceride,
Abbreviation DG) it is naturally occurring lubricant component, in the end of the year 2000, U.S. Food and Drug Administration (FDA) will by Safety Examination
Diglyceride has been included in generally recognized as safe food form.It is at present by pass with diglyceride edible oil and fat as main component
Note a kind of health-care edible oil, substitute oil with common edible rouge after have reduce interior fat, inhibit weight gain, reduce blood in
The effect of neutral fat content can be used for prevention and treatment hyperlipidemia and the cardiovascular and cerebrovascular disease closely related with hyperlipidemia
Disease, such as artery sclerosis, coronary heart disease, apoplexy, cerebral thrombosis, obesity, fatty liver.Diglyceride rich in alpha-linolenic acid can incite somebody to action
The two advantage combines, and when eating as dietary supplements, has more efficient reducing blood lipid, effect of weight reducing.
For natural animal-plant grease generally using triglycerides as main component, diacylglycerol content is very low, is usually no more than
10%.Silkworm chrysalis grease is a kind of special insect oils, is rich in ALA ingredient (25-50%) in lipid material.Silkworm chrysalis grease
Main component is also triglycerides (about 85-97%), and the content of diglyceride is usually no more than 3%.Studies have shown that with natural
Animal and plant fat is that raw material carries out partial hydrolysis or alcoholysis prepares diglyceride, is the process routes of most economy.Patent
ZL200610035743.8 discloses a kind of method of production of diglyceride using holoenzyme, using with sn-1 (3) position specificity rouge
Fat or oil is hydrolyzed in fat enzyme, and degree of hydrolysis is 20~55%, and obtained hydrolysate is obtained through molecular distillation or short-path distillation
It is the high-temperature fraction of diglyceride and triglycerides to main ingredient.Patent ZL200710030273.0 discloses a kind of glycerol two
Using the phospholipase A1 catalysis animal and plant fat with sn-1 (3) position specificity part hydrolysis occurs for the preparation method of ester,
Generate diglyceride.ZL1208305C discloses a kind of production method of diglyceride, is by ethyl alcohol or methanol and triglycerides
After mixing, partial alcoholysis reaction is carried out under immobilized lipase enzyme effect, by the isolated diglyceride of reaction product.
The study found that the ALA in silkworm chrysalis grease is more likely to be distributed in glycerol compared with other ALA type animal and plant fats
The position sn-2 of three esters.This structure feature so that its be more suitable for using the specific fat enzymatic of sn-1 (3) position moderately hydrolysis or
Alcoholysis reaction prepares diglyceride.But the phosphatide of 1.5%-2.5% is typically contained in silkworm chrysalis grease, lipase-catalyzed oil
Rouge hydrolysis is interfacial reaction, and lipase is distributed in the water phase of oil-water interfaces, and phosphatide is easier to be distributed in boundary than triglycerides
On face, hydrolysis occurs prior to triglycerides, and largely lose with the separation of water phase.Therefore, lipase-catalyzed portion
The modification for dividing hydrolysis not to be suitable for silkworm chrysalis grease.Alcoholysis reaction generally uses immobilized lipase in non-aqueous system
Middle progress avoids the hydrolysis of phosphatide, but there is also phosphatide by the risk of lipase-catalyzed alcoholysis.It is obtained moreover, extracting
Silkworm chrysalis grease in, typically contain the free fatty acid of 2-8%, seriously affected the oxidation stability of silkworm chrysalis grease, need to be anti-
It answers in process or separation process and free fatty acid content is down to level of security.
Summary of the invention
Poor for existing silkworm chrysalis oil and fat product oxidation stability, existing grease enzyme modification technology is not suitable for silkworm chrysalis oil
The modified disadvantage of rouge, the present invention provide a kind of modified silkworm chrysalis grease and preparation method thereof.In the present invention, pass through hydrophobic material
Triglycerides in fixed lipase-catalyzed silkworm chrysalis grease carries out alcoholysis reaction, and free fatty acid carries out esterification, preparation
Oxidation stability is more preferable, the more preferably modified silkworm chrysalis grease of blood fat reducing function.
The present invention to achieve the above object, is achieved through the following technical solutions:
A kind of preparation method of modified silkworm chrysalis grease, includes the following steps:
(1) using dry silkworm chrysalis as raw material, solvent extraction is utilized after crushing, obtains the extraction of grease containing silkworm chrysalis and solvent
Liquid, and moisture content is not higher than 0.5% in extract liquor；
(2) immobilized lipase is added in extract liquor, constantly addition ethyl alcohol, control reaction system moisture content is not higher than
2%, under agitation, carry out alcoholysis reaction；Wherein the triglycerides in silkworm chrysalis grease and free fatty acid distinguish generating unit
Divide second alcoholysis reaction and esterification, generates diglyceride；
(3) lipase is separated and recovered, obtained oil is mutually carried out molecular distillation, the rouge generated except dereaction by recycling design
Fat acetoacetic ester obtains modified silkworm chrysalis grease.
Preferably, step (2) described immobilized lipase is to be absorbed and fixed on hydrophobic material to have sn-1 (3) position
The lipase of specificity, the immobilization hydrophobic material are polystyrene-based macroporous absorbent resin.
Preferably, step (2) the immobilization hydrophobic material is AB-8 macropore nonpolar adsorption resin, and lipase is
Lipase T1.Immobilized lipase is preferably the Lipase T1 being absorbed and fixed on hydrophobic material.
Preferably, the additive amount of step (2) described immobilized lipase is the 1%-8% of oil quality；The addition of ethyl alcohol
Amount is the 5%-10% of oil quality.
Preferably, step (2) described ethyl alcohol in 3-4h uniformly complete by addition, and the alcoholysis reaction time is 5-8h.
Preferably, the additive amount of the ethyl alcohol is the 7%-8% of oil quality；The alcoholysis reaction time is 5-6h.
Preferably, the temperature of step (3) described molecular distillation is not higher than 140 DEG C, and operation pressure is not higher than 10Pa.
Preferably, the temperature of step (3) described molecular distillation is not higher than 130 DEG C, and operation pressure is not higher than 1Pa.
The modification silkworm chrysalis grease of above method preparation, wherein the content of triglycerides is not higher than 55%, diacylglycerol content
Not less than 45%, free fatty acid content is not higher than 0.5%, and ALA ratio is not less than 35% in total fatty acids composition.Moreover, changing
Property front and back raw material in the fat-soluble active substances such as phosphatide, vitamin E, sterol, do not lose.
This seminar the study found that silkworm chrysalis grease contains a large amount of alpha-linolenic acid, is prepared rich in alpha-linolenic acid glycerol two
The desirable feedstock of ester.But due to the unstability of alpha-linolenic acid, partial hydrolysis is carried out again after silkworm chrysalis grease is extracted refining
When, since refining process has lost a large amount of original functional components, especially phosphatide, vitamin E, sterols etc..To extract
The silkworm chrysalis grease arrived is raw material, and directly progress enzyme modification, preparation is rich in the modification silkworm chrysalis grease of ALA diglyceride.To have
The lipase adsorption of glyceride positions specificity on hydrophobic material, for petroleum ether, in ethyl alcohol enzymatic hydrolysis system when, can be to avoid
The loss of phospholipid substance.Retain the other biological activities substances such as phosphatide while modified silkworm chrysalis grease, silkworm chrysalis can be improved
The hypolipemic function of grease and the oxidation stability of product.
Compared with prior art, the beneficial effects of the present invention are:
(1) present invention is substrate using silkworm chrysalis grease, is rich in alpha-linolenic acid (ALA) by fixed lipase catalyzed preparation
Diglyceride modification silkworm chrysalis grease, improve the hypolipemic function and oxidation stability of silkworm chrysalis grease.Meanwhile modified silkworm chrysalis
The free fatty acid content of grease reduces, other activity substance contents such as vitamin E, sterol and alpha-linolenic acid phosphatide compare silkworm chrysalis oil
Raw material is high.
(2) the method for the present invention manufactures modified silkworm chrysalis grease, does not need silkworm chrysalis oil refinement, and simplify technical process,
With better economy and the feature of environmental protection.
Specific embodiment
Introduce implementation of the invention in more detail by the following examples.In the described embodiment, all percentages with
Quality meter.
Embodiment 1
The dry silkworm chrysalis (moisture content is lower than 5%) for weighing 2000g, extracts it with 2000ml petroleum ether after crushed in two times
In grease.Extract liquor (solvent and grease) and silkworm chrysalis slag are separated and recovered, is tested and analyzed, silkworm chrysalis grease 526g is obtained by extraction,
Wherein content of triglyceride is 88.3%, diacylglycerol content 1.92%, free fatty acid content 7.84%, content of phospholipid
It is 1.5%, content of vitamin E 0.029%, total sterol content is 0.15%.
30g is added in extract liquor with the lipase Lipase T1 of AB-8 resin adsorption immobilization, extract liquor is placed in
In three mouthfuls of boiling flasks of 5000ml, stirring carries out alcoholysis reaction in 35 DEG C of constant temperature blender with magnetic force.It, will using peristaltic pump
48g ethyl alcohol, is equably added in reaction system in 3h.The reaction was continued 2h after the completion of ethyl alcohol addition, then separates and recovers
Petroleum ether is recycled in lipase, (40 DEG C) vacuum evaporation of low temperature, and obtained oil is mutually carried out molecular distillation purifying.Molecular distillation (moral
State VTA company/VKL70-5FDRR molecular distillation apparatus) evaporating surface temperature is 140 DEG C, charging rate 30g/min, pressure is
16Pa.Modified silkworm chrysalis grease is obtained, through testing and analyzing, content of triglyceride 49.07%, diacylglycerol content is
48.67%, free fatty acid content 0.11%, content of phospholipid 1.93%, content of vitamin E 0.034%, total sterol contains
Amount is 0.2%.According to " measurement (accelerated oxidation test) of GB/T 21121-2007 animal and plant fat oxidation stability " method,
At 100 DEG C, its oxidation stability is assessed, obtaining OSI value is 6.27h.The product is denoted as embodiment 1.
Embodiment 2
The dry silkworm chrysalis (moisture content is lower than 5%) for weighing 2000g, extracts it with 2000ml petroleum ether after crushed in two times
In grease.Extract liquor (solvent and grease) and silkworm chrysalis slag are separated and recovered, is tested and analyzed, silkworm chrysalis grease 520g is obtained by extraction,
Wherein content of triglyceride is 88.3%, diacylglycerol content 1.92%, free fatty acid content 7.84%, content of phospholipid
It is 1.5%, content of vitamin E 0.029%, total sterol content is 0.15%.
45g is added in extract liquor with the lipase Lipase T1 of AB-8 resin adsorption immobilization, extract liquor is placed in
In three mouthfuls of boiling flasks of 5000ml, stirring carries out alcoholysis reaction in 35 DEG C of constant temperature blender with magnetic force.It, will using peristaltic pump
50g ethyl alcohol, is equably added in reaction system in 3h.The reaction was continued 2h after the completion of ethyl alcohol addition, then separates and recovers
Petroleum ether is recycled in lipase, (40 DEG C) vacuum evaporation of low temperature, and obtained oil is mutually carried out molecular distillation purifying.Molecular distillation (moral
State VTA company/VKL70-5FDRR molecular distillation apparatus) evaporating surface temperature is 130 DEG C, charging rate 30g/min, pressure is
1.4Pa.Modified silkworm chrysalis grease is obtained, through testing and analyzing, content of triglyceride 48.23%, diacylglycerol content is
49.47%, free fatty acid content 0.17%, content of phospholipid 1.97%, content of vitamin E 0.039%, total sterol contains
Amount is 0.21%.According to " measurement (accelerated oxidation test) of GB/T 21121-2007 animal and plant fat oxidation stability " method,
At 100 DEG C, its oxidation stability is assessed, obtaining OSI value is 7.03h.The product is denoted as embodiment 2.
Embodiment 3
The dry silkworm chrysalis (moisture content is lower than 5%) for weighing 2000g, extracts it with 2000ml petroleum ether after crushed in two times
In grease.Extract liquor (solvent and grease) and silkworm chrysalis slag are separated and recovered, is tested and analyzed, silkworm chrysalis grease 531g is obtained by extraction,
Wherein content of triglyceride is 88.3%, diacylglycerol content 1.92%, free fatty acid content 7.84%, content of phospholipid
It is 1.5%, content of vitamin E 0.029%, total sterol content is 0.15%.
50g is added in extract liquor with Lipozyme TL 100L (Novi's letter of AB-8 resin adsorption immobilization
Company provides), extract liquor is placed in three mouthfuls of boiling flasks of 5000ml, stirs and carries out in 35 DEG C of constant temperature blender with magnetic force
Alcoholysis reaction.50g ethyl alcohol is equably added in reaction system in 3h using peristaltic pump.Ethyl alcohol addition after the completion of after
Then continuous reaction 3h separates and recovers lipase, petroleum ether is recycled in (40 DEG C) vacuum evaporation of low temperature, and obtained oil is mutually carried out molecule
Distillation purifying.Molecular distillation (German VTA company/VKL70-5FDRR molecular distillation apparatus) evaporating surface temperature is 130 DEG C, charging
Speed is 30g/min, pressure 1.0Pa.Modified silkworm chrysalis grease is obtained, through testing and analyzing, content of triglyceride is
49.02%, diacylglycerol content 48.74%, free fatty acid content 0.15%, content of phospholipid 1.99%, vitamin E
Content is 0.037%, and total sterol content is 0.20%.According to " the survey of GB/T 21121-2007 animal and plant fat oxidation stability
Method assesses its oxidation stability at 100 DEG C calmly (accelerated oxidation test) ", and obtaining OSI value is 6.32h.The product is denoted as implementation
Example 3.
Comparative example 1
The dry silkworm chrysalis (moisture content is lower than 5%) for weighing 2000g, is extracted using 2000ml petroleum ether in two times after crushed
Grease therein.Extract liquor (solvent and grease) and silkworm chrysalis slag are separated and recovered, is tested and analyzed, in the silkworm chrysalis grease being obtained by extraction
Content of triglyceride is 88.3%, diacylglycerol content 1.92%, free fatty acid content 7.84%, and content of phospholipid is
1.5%, content of vitamin E 0.029%, total sterol content is 0.15%.Extract liquor is separated and recovered into stone under cryogenic vacuum
Oily ether obtains unmodified silkworm chrysalis grease.According to according to " the measurement of GB/T 21121-2007 animal and plant fat oxidation stability
(accelerated oxidation test) " method, at 100 DEG C, oxidation induction time (OSI) is 2.78h.The product is denoted as comparative example 1.
Comparative example 2
The dry silkworm chrysalis (moisture content is lower than 5%) for weighing 2000g, is extracted using 2000ml petroleum ether in two times after crushed
Grease therein.Extract liquor (solvent and grease) and silkworm chrysalis slag are separated and recovered, is tested and analyzed, silkworm chrysalis grease is obtained by extraction
522g, content of triglyceride is 88.3% in the silkworm chrysalis grease being obtained by extraction, diacylglycerol content 1.92%, free fatty acid
Content is 7.84%, content of phospholipid 1.5%, content of vitamin E 0.029%, and total sterol content is 0.15%.
30g is added in extract liquor with the lipase Lipase T1 (patent of 8285 adsorption of immobilization of epoxy resin
Method described in CN106906194A), extract liquor is placed in three mouthfuls of boiling flasks of 5000ml, is stirred in 35 DEG C of constant temperature magnetic force
It mixes stirring in device and carries out alcoholysis reaction.50g ethyl alcohol is equably added in reaction system in 3h using peristaltic pump.In second
The reaction was continued 2h after the completion of alcohol addition, then separates and recovers lipase, and (40 DEG C) vacuum evaporation recycling petroleum ethers of low temperature will obtain
Oil mutually carry out molecular distillation purifying.Molecular distillation (German VTA company/VKL70-5FDRR molecular distillation apparatus) evaporating surface temperature
Degree is 130 DEG C, charging rate 30g/min, pressure 1.5Pa.Modified silkworm chrysalis grease is obtained, it is sweet through testing and analyzing
Oily three ester contents are 52.6%, diacylglycerol content 45.3%, free fatty acid content 0.21%, and content of phospholipid is
0.43%, content of vitamin E 0.038%, total sterol content is 0.21%.According to " GB/T 21121-2007 animal and plant fat
The measurement (accelerated oxidation test) of oxidation stability " method at 100 DEG C assesses its oxidation stability, and obtaining OSI value is 4.61h.
The product is denoted as comparative example 2.
Comparative example 3
The dry silkworm chrysalis (moisture content is lower than 5%) for weighing 2000g, is extracted using 2000ml petroleum ether in two times after crushed
Grease therein.Extract liquor (solvent and grease) and silkworm chrysalis slag are separated and recovered, is tested and analyzed, silkworm chrysalis grease is obtained by extraction
529g, content of triglyceride is 88.3% in the silkworm chrysalis grease being obtained by extraction, diacylglycerol content 1.92%, free fatty acid
Content is 7.84%, content of phospholipid 1.5%, content of vitamin E 0.029%, and total sterol content is 0.15%.
The enzyme solution that the phospholipase A1 of 10g is added in extract liquor, extract liquor is placed in three mouthfuls of boiling flasks of 5000ml,
Stirring carries out alcoholysis reaction in 35 DEG C of constant temperature blender with magnetic force.45g ethyl alcohol is equably added in 3h using peristaltic pump
It is added in reaction system.The reaction was continued 2h after the completion of ethyl alcohol addition, then fractionation of fatty enzyme, low temperature (40 DEG C) are evaporated in vacuo back
Petroleum ether is received, obtained oil is mutually subjected to molecular distillation purifying.(German VTA company/VKL70-5 FDRR molecule steams for molecular distillation
Distillation unit) evaporating surface temperature be 130 DEG C, charging rate 30g/min, pressure 1.5Pa.Modified silkworm chrysalis grease is obtained,
Through testing and analyzing, content of triglyceride 63.2%, diacylglycerol content 43.7%, free fatty acid content is
0.37%, content of phospholipid 0.02%, content of vitamin E 0.024%, total sterol content is 0.12%.According to " GB/T
The measurement (accelerated oxidation test) of 21121-2007 animal and plant fat oxidation stability " method, at 100 DEG C, when oxidation induces
Between (OSI) be 3.54h.The product is denoted as comparative example 3.
Referring to " the function of blood fat reducing healthcare food in existing " health food is examined and assessment technique enforcement of regulations handbook "
Auxiliary lipid-lowering function comments method in energy evaluation ", and zoopery is utilized to carry out the auxiliary lipid-lowering effect of fat or oil composition.Pass through
The difference of the silkworm chrysalis grease hypolipemic function of embodiment 1-3 and comparative example 1-3 is prepared in rat test evaluation.
(1) test grouping is suitable in the environment of 23 DEG C of humidity are 55% by 80 SPF grades of Wistar rats (200g or so)
It answers 7 days, basal feed and drinking water, free feeding is all fed during adaptation.80 rats are randomly divided into 8 groups after laundering period
(blank control group, model control group, embodiment 1-3 and comparative example 1-3), every group 10, eliminate weight it is low compared with average value and
The rat of morbid state.Wherein blank group feeds basal feed during the experiment, and remaining set is fed high lipid food and (raised on 78.8% basis
Material, 1% cholesterol, 0.2% cholate 3,10% yolk powder, 10% lard).Stomach-filling processing is carried out to rat daily, it is hollow
Tween-80 solution (10mL/ (kgbw), embodiment 1-3 and the comparative example of white control group, model control group stomach-filling 2.5mmol/L
The silkworm chrysalis oil that 1-3 stomach-filling is dissolved with the Tween-80 solution of 2.5mmol/L, dosage are 2mL/ (kgbw), every group of 10 rats.
(2) model foundation with high lipid food (78.8% basal feed, 1% cholesterol, 10% lard, 10%
Yolk powder and cholate 3 of 0.2% it is raising rat 4-6 weeks continuous, so that its serum cholesterol is reached 3.77 scholar 0.41mmol/L
It models successfully.
(3) after modeling successfully, according to each group processing method nutrition purposes, especially for feeding animals shown in (1) 6 weeks i.e. after 42 days, rat was taken and is plucked
Except eyeball method takes blood, execution of then craning one.The blood of acquisition is centrifuged immediately, and upper serum is taken to measure its total cholesterol (TC), height
Density lipoprotein-cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C).
The modified silkworm chrysalis grease hypolipemic function evaluation of table 1
| Group | TC(mmol/L) | LDL-C(mmol/L) | LDL-C/HDL-C(mmol/L) |
| Blank group | 2.48±0.33d | 0.74±0.31c | 0.51±0.25d |
| Model group | 3.77±0.41a | 2.08±0.37a | 1.58±0.47a |
| Embodiment 1 | 2.01±0.35c | 0.27±0.64b | 0.19±0.12bc |
| Embodiment 2 | 2.04±0.44d | 0.36±0.22c | 0.23±0.21d |
| Embodiment 3 | 2.03±0.58d | 0.45±0.31c | 0.29±0.27d |
| Comparative example 1 | 2.34±0.58d | 0.51±0.36c | 0.34±0.37d |
| Comparative example 2 | 2.04±0.38d | 0.43±0.21c | 0.29±0.28d |
| Comparative example 3 | 2.14±0.52d | 0.53±0.37c | 0.35±0.32d |
Through animal experiment it has been observed that compared with model group, embodiment 1-3 and comparative example 1-3 stomach-filling silkworm chrysalis grease group
The ratio between low-density lipoprotein content, low-density lipoprotein and hdl concentration of rat reduce obviously, significant difference (P <
0.05)；Compared with model group, total cholesterol reduces obvious (P in embodiment 1-3 and comparative example 1-3 silkworm chrysalis grease group rat blood serum
<0.05)；Illustrate that the total cholesterol level in High fat diet rats serum can be significantly reduced in silkworm chrysalis grease and low-density lipoprotein contains
Amount.
Compared to the blank group, embodiment 2, total cholesterol level and low-density lipoprotein content difference be not significant in 3 serum
Total cholesterol level and low-density lipoprotein content difference are significant (P<0.05) in (P>0.05) and 1 serum of embodiment, illustrate logical
The silkworm chrysalis grease for crossing stomach-filling embodiment 1-3 preparation can make High fat diet rats blood lipid be restored to normal level.
Meanwhile compared to comparative example 1, total cholesterol in embodiment 1-3 stomach-filling rat blood serum, low-density lipoprotein content and
The ratio between low-density lipoprotein and hdl concentration significant difference (P < 0.05) illustrate that embodiment 1-3 lipid-lowering effect is aobvious
Work is better than comparative example 1 (unmodified silkworm chrysalis grease).The experimental results showed that the silkworm chrysalis oil that the method is prepared through the invention
Rouge has blood fat reducing function, and the silkworm chrysalis grease lipid-lowering effect that embodiment 1-3 is prepared is better than comparative example 1-3.
Claims (10)
1. a kind of preparation method of modified silkworm chrysalis grease, which comprises the steps of:
(1) using dry silkworm chrysalis as raw material, solvent extraction is utilized after crushing, obtains the extract liquor of grease containing silkworm chrysalis and solvent, and
Moisture content is not higher than 0.5% in extract liquor；
(2) immobilized lipase being added in extract liquor, constantly addition ethyl alcohol, control reaction system moisture content is not higher than 2%,
Under agitation, alcoholysis reaction is carried out；
(3) lipase is separated and recovered, obtained oil is mutually carried out molecular distillation, the fatty acid generated except dereaction by recycling design
Ethyl ester obtains modified silkworm chrysalis grease.
2. preparation method according to claim 1, which is characterized in that step (2) immobilized lipase is that absorption is solid
The lipase with sn-1 (3) position specificity being scheduled on hydrophobic material, the immobilization hydrophobic material are polystyrene
Base macroporous absorbent resin.
3. preparation method according to claim 2, which is characterized in that step (2) the immobilization hydrophobic material is AB-
8 macropore nonpolar adsorption resins, lipase are Lipase T1.
4. preparation method according to claim 3, which is characterized in that the additive amount of step (2) described immobilized lipase
For the 1%-10% of oil quality；The additive amount of ethyl alcohol is the 5%-10% of oil quality.
5. the preparation method according to claim 4, which is characterized in that step (2) described ethyl alcohol uniformly adds in 3-4h
It completes, the alcoholysis reaction time is 5-8h.
6. preparation method according to claim 5, which is characterized in that the additive amount of the ethyl alcohol is the 7%- of oil quality
8%；The alcoholysis reaction time is 5-6h.
7. preparation method described in any one according to claim 1~6, which is characterized in that step (3) described molecular distillation
Temperature is not higher than 140 DEG C, and operation pressure is not higher than 20Pa.
8. preparation method according to claim 7, which is characterized in that the temperature of step (3) described molecular distillation is not higher than
130 DEG C, operation pressure is not higher than 10Pa.
9. the modification silkworm chrysalis grease of claim 1~8 any one the method preparation.
10. modified silkworm chrysalis grease according to claim 9, which is characterized in that triglycerides in the modified silkworm chrysalis grease
Content be not higher than 55%, diacylglycerol content be not less than 45%, free fatty acid content be not higher than 0.5%, total fatty acids group
It is not less than 35% at middle ALA ratio.
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| CN1438308A (en) * | 2003-03-06 | 2003-08-27 | 华南理工大学 | Method for producing diglyceride |
| CN101225415A (en) * | 2008-01-30 | 2008-07-23 | 清华大学 | Process for preparing diglyceride by enzyme method in organic medium system |
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|---|---|---|---|---|
| CN1438308A (en) * | 2003-03-06 | 2003-08-27 | 华南理工大学 | Method for producing diglyceride |
| CN101225415A (en) * | 2008-01-30 | 2008-07-23 | 清华大学 | Process for preparing diglyceride by enzyme method in organic medium system |
| CN103361387A (en) * | 2013-07-25 | 2013-10-23 | 华南理工大学 | Production method for coproducing unsaturated monoglyceride by using diglyceride enzyme method |
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