CN109601828A - A kind of preparation method digesting oatmeal - Google Patents
A kind of preparation method digesting oatmeal Download PDFInfo
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- CN109601828A CN109601828A CN201811435984.0A CN201811435984A CN109601828A CN 109601828 A CN109601828 A CN 109601828A CN 201811435984 A CN201811435984 A CN 201811435984A CN 109601828 A CN109601828 A CN 109601828A
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- preparation
- oat
- oatmeal
- digesting
- amylase
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
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Abstract
The invention discloses a kind of preparation methods for digesting oatmeal, are related to food processing technology field, the preparation method of the enzymatic hydrolysis oatmeal, comprising the following steps: (1) will carry out boiling after oat dusting, obtain oat cooking liquor；(2) it is digested to obtain oat enzymolysis liquid using alpha-amylase immobilized；(3) it is spray-dried.The present invention is digested using alpha-amylase immobilized, and alpha-amylase can repeatedly and continuous use, the enzymatic hydrolysis for oat can obtain good hydrolysis result, and obtained enzymatic hydrolysis oatmeal delicate mouthfeel is not sticky, and beta glucan content is high.
Description
Technical field
The present invention relates to food processing technology fields, and in particular to a kind of preparation method for digesting oatmeal.
Background technique
Oat is a kind of rain fed crops of characteristic, and oat is a kind of crop for having both dietotherapy.Protein, rouge in oat
Fat, mineral matter element total amount and unsaturated fatty acid content occupy first of cereal, especially belong to the β-of water-soluble dietary fiber
Glucan content highest in all cereal.FDA assert that avenabeta glucosan has the effect of reducing cholesterol and steady blood glucose.
There are the products such as oatmeal, sowens, fast-food oat powder and enzymatic hydrolysis oatmeal for a series of food of oat exploitation.With it is undressed
Grains of oats compare, digest the easy to use of oatmeal, can be used to make Flour product, cake, drink etc. and widely be closed
Note.Some researches show that, oat is digested using amylase, can significantly improve gallic acid in oat, p-Coumaric Acid,
The content of the extractibilities such as ferulic acid total polyphenols and free phenol, therefore, enzymatic hydrolysis oatmeal have more preferably body anti-oxidation function.
Immobilised enzymes refers to plays catalytic action in certain spatial dimension, and can repeatedly with the enzyme of continuous use.Usually
Enzymic catalytic reaction all carries out in aqueous solution, and immobilised enzymes is to make water-soluble enzyme with either physically or chemically handling
Become it is not soluble in water, but still with enzymatic activity state.In the prior art, the report about enzymatic hydrolysis oatmeal is less,
The report of oatmeal is not digested using enzyme immobilization technology, and a kind of pre-enzymatic oat powder is disclosed in CN102845677A
Preparation method, comprising: amylase is uniformly mixed with water and is admixed in oat meal, the mixture made later is swollen through squeezing
Change and dry, finally wears into fine powder.The disadvantages of that there are hydrolysis results is poor for this method, and product taste and flavor is poor.?
A kind of enzyme solution of oat is disclosed in CN107647285A, oat seed is mainly first passed through into germination treatment, is being carried out
The shortcomings that defibrination and enzymatic hydrolysis, this mode, is that the requirement of oat raw material is high, and germination process will cause beta glucan substantially
Degree loss.
Summary of the invention
Aiming at the deficiencies in the prior art, the present invention provides a kind of preparation method for digesting oatmeal, using fixation
Change alpha-amylase to digest oat, good hydrolysis result can be obtained, obtained enzymatic hydrolysis oatmeal delicate mouthfeel does not glue
Thick, beta glucan content is high, due to alpha-amylase be fixed it is rear not soluble in water, can also repeatedly and continuous use, can
Realize continuous production.
In order to reach above-mentioned purpose of the present invention, the invention adopts the following technical scheme:
The present invention provides a kind of preparation method for digesting oatmeal, comprising the following steps:
(1) boiling will be carried out after oat dusting, obtains oat cooking liquor；
(2) it is digested to obtain oat enzymolysis liquid using alpha-amylase immobilized；
(3) it is spray-dried.
It preferably, further include that the oat enzymolysis liquid is concentrated before step (3) described spray drying.
It is highly preferred that obtaining concentration fluid solid content after the concentration is 30~40%.The concentration uses double effect evaporator
It is concentrated, vacuum degree is -0.06~-0.08MPa, and one effect temperature of concentration is 80~90 DEG C, and two effect temperature are 60~70 DEG C.
In the present invention, the amylase in the alpha-amylase immobilized can use mesophilicα-diastase or high temperature alphalise starch
Enzyme.Mesophilicα-diastase is fixed on glutaraldehyde cross-linking chitosan microball by preparation method using cross-linking method.Glutaraldehyde
Chitosan microball has preferable fixed adsorption effect to mesophilicα-diastase, and alpha-amylase is not easy free precipitation, while energy
The stability for enough improving alpha-amylase, extend enzyme uses the time, since alpha-amylase is through immobilization, so that alpha-amylase is by swimming
Amorph is converted into stationary state, and therefore, gained oat liquid is not required to carry out destroy the enzyme treatment after enzymatic hydrolysis, enormously simplifies production technology, by
In the repeatable and continuous use of immobilised enzymes, continuous production is realized.In addition, the mesophilicα-diastase bought in the market is simultaneously
It is impure, it will usually to contain the other biologicals metabolite such as a small amount of protease, 1,4 beta-glucanase.1,4 beta-glucanase therein can urge
Change the beta glucan in hydrolysis oat cell wall, this may be the reason that beta glucan content is low in common enzymatic hydrolysis oatmeal.It adopts
The alpha-amylase immobilized obtained with method of the invention, since glutaraldehyde chitosan microball is to the selectivity of mesophilicα-diastase
Suction-operated improves the product purity of alpha-amylase, therefore the content obtained for digesting beta glucan in oatmeal product
It is higher.
Preferably, step (1) described dusting includes being crushed oat in micronizer to obtain oat fine powder, so
Oat fine powder is crossed into 100~150 mesh afterwards.It is uniformly and thin that micronizer crushes the obtained particle size distribution of oat, nutrition at
Divide loss few, can preferably be dispersed in water, shorten the time required for starch gelatinization, the cooking liquor obtained after boiling is uniformly thin
It is greasy.
Preferably, the temperature of step (1) described boiling is 90~100 DEG C, and the digestion time is 15~30min.To powder
Oatmeal after change carries out boiling, the starch in oat can rapid gelatinisation, and be gelatinized very complete.
Preferably, the material-water ratio of step (1) described boiling is 1:(4~6).
Preferably, step (2) the enzymatic hydrolysis equipment is jacket type fixed bed reactors.
Preferably, the raw material flow rate of the jacket type fixed bed reactors is 1.0~3.5L/min.
Preferably, 160~170 DEG C of inlet air temperature of step (3) described spray drying, 90~100 DEG C of temperature, air draft in tower
90~100 DEG C.Compared with the method for roller drying, obtained oatmeal is spray-dried due to the oat enzymatic hydrolysis in spray process
Liquid is come into full contact with thermal current, and powder is looser, and partial size is big and is evenly distributed, dissolution and dispersibility in water
It can be more preferable, it is not easy to precipitate, therefore, the oatmeal being spray-dried has better instant capacity, is particularly suited for drink
Processing.
Compared with prior art, the invention has the advantages that:
(1) enzyme preparation utilization rate is high: alpha-amylase immobilized provided by the invention has fixed adsorption effect well, α-
Amylase is not easy free precipitation, and alpha-amylase is repeatable and continuous use, can be realized continous way production, and after digesting also not
It needs to carry out destroy the enzyme treatment.
(2) present invention digests oat using alpha-amylase immobilized in fixed bed reactors, obtained production
Product beta glucan content is high, can reach 4.5% or so, much higher than conventional enzyme solution.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1: the preparation of alpha-amylase immobilized
The acetic acid solution of 2% volumetric concentration is added chitosan into, the chitosan acetic acid that 2% (w/v) is made sufficiently is dissolved
Chitosan acetic acid solution is added dropwise in the sodium hydroxide solution of isometric 2mol/L dropwise, chitosan is collected by filtration by solution
White microballoon, distilled water repeated flushing to water lotion is neutrality, and after placing for 24 hours at room temperature, which is added
Into the glutaraldehyde solution of 2% (v/v), the mass volume ratio of chitosan white microballoon and glutaraldehyde solution is 1:10, will be mixed
Liquid first stands 5h in 35 DEG C of water-bath concussion 1h postpositions at room temperature, filters, distilled water flushing obtains yellow to cleaning solution neutrality
Mesophilicα-diastase is made into 0.1mg/ with dibastic sodium phosphate-phosphate sodium dihydrogen buffer solution of 50mmol/L by crosslinked chitosan microsphere
The enzyme solutions of ml, enzyme solutions and crosslinked chitosan microsphere are that 5:1 is mixed according to volume mass ratio, are placed in shaking flask device and vibrate
2h, filtering are adsorbed, dibastic sodium phosphate-phosphate sodium dihydrogen buffer solution of 50mmol/L washs to obtain alpha-amylase immobilized.
Embodiment 2: the preparation of oatmeal is digested
Oat smashes it through 120 meshes using micronizer and obtains oatmeal.Oatmeal is put into cooker, swallow is added
The water of 5 times of flour amounts (quality) carries out boiling 20min at 98 DEG C and obtains oat cooking liquor after mixing.
In the alpha-amylase immobilized that jacket type fixed bed reactors (internal diameter 40cm) filling embodiment 1 is prepared, enzyme
Filling height be 60cm, jacket temperature is 80 DEG C, oat cooking liquor is continuously entered from upper end with the flow velocity of 2.5L/min solid
Fixed bed immobilizes enzyme digestion reaction, collects oat enzymolysis liquid.Enzymolysis liquid be concentrated in double effect evaporator (vacuum degree be-
0.08MPa, concentration one effect temperature be 85 DEG C, two effect 65 DEG C) to solid content be 35%.Concentrate is sprayed through atomizer tower
Mist is dry, controls 160~170 DEG C of spray tower inlet air temperature, and 90~100 DEG C of temperature in tower, collect drying by 90~100 DEG C of air draft
Enzymatic hydrolysis oatmeal afterwards, beta glucan content is 4.53% (using beta glucan measurement examination in obtained oatmeal after measured
Agent box is measured).
Embodiment 3: the preparation of oatmeal is digested
Oat smashes it through 100 meshes using micronizer and obtains oatmeal.Oatmeal is put into cooker, swallow is added
The water of 4 times of flour amounts (quality) carries out boiling 15min at 100 DEG C and obtains oat cooking liquor after mixing.
In the alpha-amylase immobilized that jacket type fixed bed reactors (internal diameter 40cm) filling embodiment 1 is prepared, enzyme
Filling height be 60cm, jacket temperature is 80 DEG C, oat cooking liquor is continuously entered from upper end with the flow velocity of 1.0L/min solid
Fixed bed immobilizes enzyme digestion reaction, collects oat enzymolysis liquid.Enzymolysis liquid be concentrated in double effect evaporator (vacuum degree be-
0.08MPa, one effect temperature of concentration is 85 DEG C, two 65 DEG C of effects) to solid content 40%.Concentrate is sprayed through atomizer tower
It is dry, 160~170 DEG C of spray tower inlet air temperature of control, 90~100 DEG C of temperature in tower, 90~100 DEG C of air draft, after collecting drying
Enzymatic hydrolysis oatmeal, after measured in obtained oatmeal beta glucan content be 4.49%.
Embodiment 4: the preparation of oatmeal is digested
Oat smashes it through 150 meshes using micronizer and obtains oatmeal.Oatmeal is put into cooker, swallow is added
The water of 6 times of flour amounts (quality) carries out boiling 30min at 90 DEG C and obtains oat cooking liquor after mixing.
In the alpha-amylase immobilized that jacket type fixed bed reactors (internal diameter 40cm) filling embodiment 1 is prepared, enzyme
Filling height be 60cm, jacket temperature is 80 DEG C, oat cooking liquor is continuously entered from upper end with the flow velocity of 3.5L/min solid
Fixed bed immobilizes enzyme digestion reaction, collects oat enzymolysis liquid.Enzymolysis liquid be concentrated in double effect evaporator (vacuum degree be-
0.08MPa, one effect temperature of concentration is 85 DEG C, two 65 DEG C of effects) to solid content 30%.Concentrate is sprayed through atomizer tower
It is dry, 160~170 DEG C of spray tower inlet air temperature of control, 90~100 DEG C of temperature in tower, 90~100 DEG C of air draft, after collecting drying
Enzymatic hydrolysis oatmeal, after measured in obtained oatmeal beta glucan content be 4.65%.
Comparative example
The preparation that enzymatic hydrolysis oatmeal is carried out using conventional method, the distinctive points with 2 scheme of embodiment are as follows: by oat boiling
Enzymatic vessel is added in liquid, and the additional amount of alpha-amylase (medium temperature amylase) is the 0.2% of oat cooking liquor quality, digests at 80 DEG C
90min, the enzyme deactivation 10min at 121 DEG C, remaining step is with embodiment 2, and gained digests beta glucan content in oatmeal after measured
It is 3.20%.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. a kind of preparation method for digesting oatmeal, which comprises the following steps:
(1) boiling will be carried out after oat dusting, obtains oat cooking liquor；
(2) it is digested to obtain oat enzymolysis liquid using alpha-amylase immobilized；
(3) it is spray-dried.
2. a kind of preparation method for digesting oatmeal according to claim 1, which is characterized in that step (3) is described spraying
It further include that concentrate is concentrated to give to the oat enzymolysis liquid before drying.
3. a kind of preparation method for digesting oatmeal according to claim 2, which is characterized in that consolidating for the concentrate contains
Amount is 30~40%.
4. a kind of preparation method for digesting oatmeal according to claim 1 or 2, which is characterized in that the immobilization α-
Amylase the preparation method comprises the following steps: mesophilicα-diastase is fixed on glutaraldehyde cross-linking chitosan microball using cross-linking method.
5. a kind of preparation method for digesting oatmeal according to claim 1, which is characterized in that step (1) described dusting
Including being crushed oat in micronizer to obtain oat fine powder, oat fine powder is then crossed into 100~150 mesh.
6. a kind of preparation method for digesting oatmeal according to claim 1, which is characterized in that step (1) described boiling
Temperature be 90~100 DEG C, the digestion time be 15~30min.
7. a kind of preparation method for digesting oatmeal according to claim 1, which is characterized in that step (1) described boiling
Material-water ratio be 1:(4~6).
8. a kind of preparation method for digesting oatmeal according to claim 4, which is characterized in that step (2) described enzymatic hydrolysis
Equipment is jacket type fixed bed reactors.
9. a kind of preparation method for digesting oatmeal according to claim 8, which is characterized in that the jacket type fixed bed
The raw material flow rate of reactor is 1.0~3.5L/min.
10. a kind of preparation method for digesting oatmeal according to claim 1, which is characterized in that step (3) is described spraying
160~170 DEG C of dry inlet air temperature, 90~100 DEG C of temperature in tower, 90~100 DEG C of air draft.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117652618A (en) * | 2023-10-25 | 2024-03-08 | 无锡赞匠生物科技有限公司 | Enzymolysis oat flour and preparation method thereof |
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| CN117652618A (en) * | 2023-10-25 | 2024-03-08 | 无锡赞匠生物科技有限公司 | Enzymolysis oat flour and preparation method thereof |
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Application publication date: 20190412 |