CN109601537A - One plant growth regulators and its method of administration - Google Patents

One plant growth regulators and its method of administration Download PDF

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Publication number
CN109601537A
CN109601537A CN201811522115.1A CN201811522115A CN109601537A CN 109601537 A CN109601537 A CN 109601537A CN 201811522115 A CN201811522115 A CN 201811522115A CN 109601537 A CN109601537 A CN 109601537A
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CN
China
Prior art keywords
plant growth
sodium
agent
activator protein
toverticilliumdahliaactivitie activitie
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CN201811522115.1A
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Chinese (zh)
Inventor
陈昶
徐军
马超
张刚应
赵宜君
王佰涛
段小莉
张力卜
李佳浩
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Hebei Zhong Bao Green Crop Technology Co Ltd
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Hebei Zhong Bao Green Crop Technology Co Ltd
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Publication of CN109601537A publication Critical patent/CN109601537A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46N-acyl derivatives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/16Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
    • A01N33/18Nitro compounds
    • A01N33/20Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
    • A01N33/22Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to technical field of pesticide more particularly to a plant growth regulators and its method of administration, effective component includes toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, any one in compound sodium nitrophenolate.Plant growth regulator and its wettable powder provided by the invention, water dispersible granules, suspending agent and aqua use toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, any one mutual compatibility in compound sodium nitrophenolate is as effective component, its mechanism of action is different, rice is directed to by the use concentration and application method after compounding in restriction, corn, wheat, peanut, cotton, vegetables, tobacco, fruit tree, gardens, there is synergistic effect under the various crops such as flowers, increasing both production and income, and there is apparent effect of gain for the fruit of fruit tree, effectively improve chlorophyll content of plant, it protects green anti-old, improve sugared content, promote plant growth, effectively improve the quality of fruit.

Description

One plant growth regulators and its method of administration
Technical field
The invention belongs to technical field of pesticide more particularly to a plant growth regulators and its method of administration.
Background technique
Plant growth regulator is a kind of pesticide for coordinate plant growth development, is had naturally including artificial synthesized The compound of plant hormone similar action and the natural phytohormone extracted from biology.Plant growth regulator is in low concentration The growth and development of plant can be adjusted down, can be used for regulating and controlling the synthesis of plant intracorporal nucleic acid, protein and enzyme, it can be right Different phase in plant growth and development process such as germinates, takes root, cell elongation, Organ Differentiation, bud differentiation, blooming, result, Regulation and control is played the role of in fallen leaves, suspend mode etc..The content that plant intracorporal protein and carbohydrate can be improved can promote plant and change Deformation states can enhance the ability of plant cold resistance, drought resisting, salt resistance alkali and disease and insect resistance.Have been widely used for grain, vegetables, fruit tree, Forest, flowers and other crops achieve significant economic benefit, play a role to promotion agricultural production.
Every plant growth regulators have specific purposes, and crops require to compare to the concentration of plant growth regulator Strictly, concentration is excessive, will cause blade getting fat and becomes fragile, and occurs that lopsided blade is withered to fall off or even complete stool is dead;Concentration is too small, Due effect is then not achieved.Plant growth regulator has many purposes, difference due to kind and target plant.
For conventional use of plant growth regulator in the prior art, there are larger difference, middle parts in using effect Divide and have the following problems: plant growth regulator, which exists to include total nutrient and do not strengthened, can not meet plant nutrient demand; Raw material components compatibility is unreasonable, and each component is different to plant effect, and compatibility is unable to reach synergy production-increasing function, especially for fruit Effect of gain is played in fact.
Summary of the invention
The present invention for the above technical issues, proposes a plant growth regulators.
In order to achieve the above object, the technical solution adopted by the present invention are as follows:
One plant growth regulators, effective component include toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, answer Any one in nitre phenol sodium.
As preferred: the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, compound sodium nitrophenolate weight ratio be respectively 1:(0.0005~50), 1:(0.001~100) and 1:(0.0001~50).
As preferred: the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, compound sodium nitrophenolate weight ratio be respectively 1:(0.001~40), 1:(0.01~50) and 1:(0.005~40).(the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, jade Meter Su, compound sodium nitrophenolate weight ratio be respectively 1:(0.05~20), 1:(0.02~20) and 1:(0.01~10).)
As preferred: the effective component accounts for the 0.01~90% of the plant growth regulator gross mass.
As preferred: the effective component accounts for the 0.2~20% of the plant growth regulator gross mass.
As preferred: the dosage form of the plant growth regulator is wettable powder, water dispersible granules, suspending agent and water Any one of agent.
The wettable powder of one plant growth regulators, the wettable powder include the raw material of following weight percent Component: 0.01%~80% effective component, 1%~15% dispersing agent, 1%~6% wetting agent, filler complement to 100%; The effective component include weight ratio be 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, 1:(0.001~ 100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1:(0.0001~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and compound nitrophenol One of sodium;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, detergent alkylate sulphur Any one or more of sour calcium and polycarboxylate;The wetting agent includes Succinate sodium sulfonate, sldium lauryl sulfate and two Any one or more of sodium isobutyl naphthalene sulfonate pull open powder;The filler include kaolin, diatomite, attapulgite, cover it is de- Any one or more of soil, bentonite, clay, talcum powder, white carbon black, calcium carbonate and starch.
The water dispersible granules of one plant growth regulators, the water dispersible granules include the raw material of following weight percent Component: 0.01%~80% effective component, 5%~15% dispersing agent, 2%~8% wetting agent, 0~5% disintegration Agent, 0~5% binder, filler complement to 100%;The effective component include weight ratio be 1:(0.0005~50) pole Alternaria tenuis activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1: One of the toVerticilliumdahliaActivitie Activitie S of Relative activator protein of (0.0001~50) and compound sodium nitrophenolate;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, detergent alkylate sulphur Any one or more of sour calcium and polycarboxylate, wetting agent include Succinate sodium sulfonate, sldium lauryl sulfate and two isobutyls Any one or more of base naphthalene sulfonic acids;The disintegrating agent include urea, ammonium sulfate, sodium chloride, sodium carbonate, in sodium alginate It is any one or several;The binder includes appointing in sucrose, glucose, maltose, dextrin, polyvinyl alcohol and polyethylene glycol It is one or more of;The inert solid carrier includes kaolin, diatomite, attapulgite, montmorillonite, bentonite, clay, talcum Any one or more of powder, white carbon black, calcium carbonate and starch.
The suspending agent of one plant growth regulators, the suspending agent include the raw material components of following weight percent: 0.01%~50% effective component, 2%~8% dispersing agent, 1%~4% wetting agent, 0~2% thickener, 0~ 5% antifreezing agent, 0.01~0.05% defoaming agent, deionized water complement to 100%;The effective component includes that weight ratio is 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein With zeatin or 1:(0.0001~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and one of compound sodium nitrophenolate;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, detergent alkylate sulphur Any one or more of sour calcium and polycarboxylate;The wetting agent includes Succinate sodium sulfonate, sldium lauryl sulfate, two different Any one or more of sodium butylnaphthalenesulfonate and alkyl phenol polyoxyethylene ether;The thickener include xanthan gum, aluminium-magnesium silicate, Sodium carboxymethylcellulose, hydroxyethyl cellulose, gelatin, Arabic gum, polyvinyl alcohol, polyvinylpyrrolidone and polyvinyl acetate Any one or more of ester;The antifreezing agent includes in ethylene glycol, propylene glycol, glycerine, urea, sodium chloride and calcium chloride It is any one or several;The defoaming agent includes polyether antifoam agent and/or organic silicon defoamer.
The aqua of one plant growth regulators, the aqua include the raw material components of following weight percent: 0.01% ~50% effective component, cosolvent 0~5%, emulsifier 1~15%, antifreezing agent 0~5%, defoaming agent 0.01~0.05%, Deionized water complements to 100%;The effective component include weight ratio be 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activate egg White and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1:(0.0001~50) superfine chain One of lattice spore activator protein and compound sodium nitrophenolate;
The cosolvent includes toluene, dimethylbenzene, solvent naphtha, methanol, ethyl alcohol, isopropanol, acetone, acetonitrile, cyclohexanone, N- Any one or more of methyl pyrrolidone and decyl amide;The emulsifier includes anhydrous sorbitol APEO, fat Alcohol polyoxyethylene ether, polyoxyethylene sorbitan ether monoleate, styrylphenol polyoxyethylene ether, dibenzyl phenol polyoxy Any one or more of vinethene, aliphatic amine polyoxyethylene ether and calcium dodecyl benzene sulfonate;The antifreezing agent includes second two Any one or more of alcohol, propylene glycol, glycerine, urea, sodium chloride, calcium chloride;The defoaming agent includes polyether antifoam agent And/or organic silicon defoamer.
A kind of method of administration of above-mentioned plant growth regulator, the application concentration of the plant growth regulator is 1.0~ 20ppm applies using seed dressing, seed soaking, root dipping, pouring root or by the way of spraying, or using in seed dressing, seed soaking, root dipping or pouring root It is sprayed simultaneously in the plant growth critical period 2~3 times.
Compared with prior art, the advantages and positive effects of the present invention are:
Plant growth regulator and its wettable powder provided by the invention, water dispersible granules, suspending agent and aqua use ToVerticilliumdahliaActivitie Activitie S of Relative activator protein and any one mutual compatibility of diethyl aminoethyl hexanoate, zeatin, compound sodium nitrophenolate are as effective component, effect Mechanism is different, and toVerticilliumdahliaActivitie Activitie S of Relative activator protein can cause a system in plant in conjunction with the receptor protein on plant cell membrane Column related enzyme activity increases and the enhancing of gene expression amount, is remarkably improved the content (amber of the intracorporal succinate dehydrogenase of plant Amber acidohydrogenase is connection one of oxidative phosphorylation and the hinge of electron transmission, can be thin for eukaryocyte mitochondria and a variety of protokaryons The aerobic respiratory chain with production capacity of born of the same parents provides electronics, and activity may generally serve as the index of evaluation tricarboxylic acid cycle operation degree), promote Into plant absorption, synthesis and metabolic capability.DA-6 is not plant hormone, but adjustable intracorporal hormone after plant absorption Activity promotes plant growth and degeneration-resistant.Zeatin can promote plant cell division, prevent chlorophyll and protein degradation, slow down Respiration keeps cell viability.Compound sodium nitrophenolate mainly promotes the flowing of plant cell plasm, to improve cell viability, promotees Into plant establishment and increase production degeneration-resistant etc..After compounding, toVerticilliumdahliaActivitie Activitie S of Relative activator protein can greatly facilitate the suction of other several substances It receives, it is maximized to absorb and utilize each plant growth regulators, it plays a role in plant jointly, to play synergy work With, and effectively improve crop yield.By compounding after restriction use concentration and application method be directed to rice, corn, wheat, There is synergistic effect, increasing both production and income under the various crops such as peanut, cotton, vegetables, tobacco, fruit tree, gardens, flowers, and be directed to fruit The fruit of tree has apparent effect of gain.It is remarkably improved crop chlorophyll content, improves quality of agricultural product.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects It encloses.
The embodiment of the invention provides a plant growth regulators, effective component includes toVerticilliumdahliaActivitie Activitie S of Relative activator protein With any one in diethyl aminoethyl hexanoate, zeatin, compound sodium nitrophenolate.Using toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, answer For any one mutual compatibility of nitre phenol sodium as effective component, the mechanism of action is different, passes through the use concentration after compounding in restriction It is directed under the various crops such as rice, corn, wheat, peanut, cotton, vegetables, tobacco, fruit tree, gardens, flowers and has with application method There are synergistic effect, increasing both production and income, and there is apparent effect of gain for the fruit of fruit tree.Crop chlorophyll is remarkably improved to contain Amount improves quality of agricultural product.
Wherein toVerticilliumdahliaActivitie Activitie S of Relative activator protein is to utilize reverse-genetics approach from Alternaria tenuissima (Alterneria Tenuissima new function activator protein gene peaT1 is cloned in), utilizes the engineering strain E.coli of high efficient expression PeaT1 and P.pastoris PeaT1, fermented, extraction a kind of biologically active protein, by 207 amino acid groups At.Activator protein and other technologies are with the use of reaching 65% or more to capsicum, tomato and tobacco virus control effect, simultaneously Have the function of significantly volume increase and improving quality.After touching plant organ surface, can on plant cell membrane by Body protein combines, and causes the enhancing of a series of related enzyme activities increases and gene expression amount in plant, excites one in plant Range of metabolic regulation promotes plant growth, improves chlorophyll content, improves crop yield.
In an alternative embodiment: the weight of the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, compound sodium nitrophenolate Than being respectively 1:(0.0005~50), 1:(0.001~100) and 1:(0.0001~50).
In an alternative embodiment: the weight of the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, zeatin, compound sodium nitrophenolate Than being respectively 1:(0.001~40), 1:(0.01~50) and 1:(0.005~40).(the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and amine Fresh ester, zeatin, compound sodium nitrophenolate weight ratio be respectively 1:(0.05~20), 1:(0.02~20) and 1:(0.01~10).)
In an alternative embodiment: the effective component accounts for the 0.01~90% of the plant growth regulator gross mass.
In an alternative embodiment: the effective component accounts for the 0.2~20% of the plant growth regulator gross mass.
In an alternative embodiment: the dosage form of the plant growth regulator is wettable powder, water dispersible granules, hangs Floating any one of agent and aqua.According to the difference of plant growth regulator dosage form, different auxiliary agents can be used, these auxiliary agents Known in those skilled in the art: as dispersing agent, wetting agent, emulsifier, thickener, antifreezing agent, stabilizer, defoaming agent, One or more of filler.
The wettable powder of one plant growth regulators, the wettable powder include the raw material of following weight percent Component: 0.01%~80% effective component, 1%~15% dispersing agent, 1%~6% wetting agent, filler complement to 100%; The effective component include weight ratio be 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, 1:(0.001~ 100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1:(0.0001~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and compound nitrophenol One of sodium;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, detergent alkylate sulphur Any one or more of sour calcium and polycarboxylate;The wetting agent includes Succinate sodium sulfonate, sldium lauryl sulfate and two Any one or more of sodium isobutyl naphthalene sulfonate pull open powder;The filler include kaolin, diatomite, attapulgite, cover it is de- Any one or more of soil, bentonite, clay, talcum powder, white carbon black, calcium carbonate and starch.
The water dispersible granules of one plant growth regulators, the water dispersible granules include the raw material of following weight percent Component: 0.01%~80% effective component, 5%~15% dispersing agent, 2%~8% wetting agent, 0~5% disintegration Agent, 0~5% binder, filler complement to 100%;The effective component include weight ratio be 1:(0.0005~50) pole Alternaria tenuis activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1: One of the toVerticilliumdahliaActivitie Activitie S of Relative activator protein of (0.0001~50) and compound sodium nitrophenolate;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, detergent alkylate sulphur Any one or more of sour calcium and polycarboxylate, wetting agent include Succinate sodium sulfonate, sldium lauryl sulfate and two isobutyls Any one or more of base naphthalene sulfonic acids;The disintegrating agent include urea, ammonium sulfate, sodium chloride, sodium carbonate, in sodium alginate It is any one or several;The binder includes appointing in sucrose, glucose, maltose, dextrin, polyvinyl alcohol and polyethylene glycol It is one or more of;The inert solid carrier includes kaolin, diatomite, attapulgite, montmorillonite, bentonite, clay, talcum Any one or more of powder, white carbon black, calcium carbonate and starch.
The suspending agent of one plant growth regulators, the suspending agent include the raw material components of following weight percent: 0.01%~50% effective component, 2%~8% dispersing agent, 1%~4% wetting agent, 0~2% thickener, 0~ 5% antifreezing agent, 0.01~0.05% defoaming agent, deionized water complement to 100%;The effective component includes that weight ratio is 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein With zeatin or 1:(0.0001~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and one of compound sodium nitrophenolate;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, detergent alkylate sulphur Any one or more of sour calcium and polycarboxylate;The wetting agent includes Succinate sodium sulfonate, sldium lauryl sulfate, two different Any one or more of sodium butylnaphthalenesulfonate and alkyl phenol polyoxyethylene ether;The thickener include xanthan gum, aluminium-magnesium silicate, Sodium carboxymethylcellulose, hydroxyethyl cellulose, gelatin, Arabic gum, polyvinyl alcohol, polyvinylpyrrolidone and polyvinyl acetate Any one or more of ester;The antifreezing agent includes in ethylene glycol, propylene glycol, glycerine, urea, sodium chloride and calcium chloride It is any one or several;The defoaming agent includes polyether antifoam agent and/or organic silicon defoamer.
The aqua of one plant growth regulators, the aqua include the raw material components of following weight percent: 0.01% ~50% effective component, cosolvent 0~5%, emulsifier 1~15%, antifreezing agent 0~5%, defoaming agent 0.01~0.05%, Deionized water complements to 100%;The effective component include weight ratio be 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activate egg White and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1:(0.0001~50) superfine chain One of lattice spore activator protein and compound sodium nitrophenolate;
The cosolvent includes toluene, dimethylbenzene, solvent naphtha, methanol, ethyl alcohol, isopropanol, acetone, acetonitrile, cyclohexanone, N- Any one or more of methyl pyrrolidone and decyl amide;The emulsifier includes anhydrous sorbitol APEO, fat Alcohol polyoxyethylene ether, polyoxyethylene sorbitan ether monoleate, styrylphenol polyoxyethylene ether, dibenzyl phenol polyoxy Any one or more of vinethene, aliphatic amine polyoxyethylene ether and calcium dodecyl benzene sulfonate;The antifreezing agent includes second two Any one or more of alcohol, propylene glycol, glycerine, urea, sodium chloride, calcium chloride;The defoaming agent includes polyether antifoam agent And/or organic silicon defoamer.
A kind of method of administration of above-mentioned plant growth regulator, the application concentration of the plant growth regulator is 1.0~ 20ppm applies using seed dressing, seed soaking, root dipping, pouring root or by the way of spraying, or using in seed dressing, seed soaking, root dipping or pouring root It is sprayed simultaneously in the plant growth critical period 2~3 times.
Plant growth regulator and its method of administration provided by the embodiment of the present invention are introduced in detail in order to become apparent from, under Face will be described in conjunction with specific embodiments.
The wettable powder of 1: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 3.5%, diethyl aminoethyl hexanoate 0.5%, sodium lignin sulfonate 8%, sldium lauryl sulfate 4%, kaolin are mended Foot obtains the plant growth regulator of the present embodiment to 100% after being pulverized and mixed.
The wettable powder of 2: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.2%, diethyl aminoethyl hexanoate 8%, sodium methylene bis-naphthalene sulfonate 6%, Succinate sodium sulfonate 5%, hard charcoal Black 5%, diatomite complements to 100%, and the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The wettable powder of 3: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 5%, diethyl aminoethyl hexanoate 10%, calcium lignosulfonate 10%, pull open powder 4%, precipitated calcium carbonate complements to 100%, the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The wettable powder of 4: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 3%, zeatin 5%, calcium dodecyl benzene sulfonate 7%, Succinate sodium sulfonate 5%, bentonite 100% is complemented to, the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The wettable powder of 5: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.3%, zeatin 15%, sodium lignin sulfonate 5%, pull open powder 5%, kaolin complements to 100%, the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The wettable powder of 6: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 4.8%, zeatin 0.2%, sodium methylene bis-naphthalene sulfonate 8%, sldium lauryl sulfate 3% are concave-convex Stick soil complements to 100%, and the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The wettable powder of 7: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 2.94%, compound sodium nitrophenolate 0.06%, polycarboxylate 4%, pull open powder 4%, starch complements to 100%, the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The wettable powder of 8: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 2%, compound sodium nitrophenolate 2%, sodium lignin sulfonate 12%, Succinate sodium sulfonate 2%, starch 10%, clay complements to 100%, and the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The wettable powder of 9: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.1%, compound sodium nitrophenolate 2%, calcium lignosulfonate 7%, pull open powder 6%, diatomite complements to 100%, the plant growth regulator of the present embodiment is obtained after being pulverized and mixed.
The water dispersible granules of 10: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 4.2%, diethyl aminoethyl hexanoate 0.3%, sodium methylene bis-naphthalene sulfonate 6%, sldium lauryl sulfate 4%, urea 5%, glucose 5%, kaolin complements to 100%.It is uniformly mixed, mediates be granulated the plant life for obtaining the present embodiment after crushed Long regulator.
The water dispersible granules of 11: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 2.4%, zeatin 12.6%, polycarboxylate 5%, Succinate sodium sulfonate 4%, ammonium sulfate 4%, Sucrose 4%, starch complements to 100%.It is uniformly mixed, mediates granulation after crushed and obtain the plant growth regulating of the present embodiment Agent.
The water dispersible granules of 12: one plant growth regulators of embodiment, the raw material components including following weight percent: ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 5.4%, compound sodium nitrophenolate 0.6%, sodium lignin sulfonate 12%, pull open powder 3%, sodium carbonate 4%, paste Essence 3%, diatomite complements to 100%.It is uniformly mixed, mediates granulation after crushed and obtain the plant growth regulating of the present embodiment Agent.
The suspending agent of 13: one plant growth regulators of embodiment, the raw material components including following weight percent: superfine Rod method Activating protein-1 %, diethyl aminoethyl hexanoate 11%, sodium lignin sulfonate 2%, sodium methylene bis-naphthalene sulfonate 3%, succinate sulfonic acid Sodium 2%, xanthan gum 0.2%, propylene glycol 4%, organic silicon defoamer 0.02%, deionized water complements to 100%.It is uniformly mixed, The plant growth regulator of the present embodiment is obtained after being sanded.
The suspending agent of 14: one plant growth regulators of embodiment, the raw material components including following weight percent: superfine Rod method Activating protein-1 %, zeatin 1%, polycarboxylate 3%, alkyl phenol polyoxyethylene ether 3%, xanthan gum 0.25%, silicic acid Magnalium 0.5%, ethylene glycol 3%, organic silicon defoamer 0.02%, deionized water complements to 100%.It is uniformly mixed, after being sanded Obtain the plant growth regulator of the present embodiment.
The suspending agent of 15: one plant growth regulators of embodiment, the raw material components including following weight percent: superfine Rod method activator protein 2%, compound sodium nitrophenolate 8%, sodium methylene bis-naphthalene sulfonate 4%, pull open powder 1%, xanthan gum 0.1%, silicic acid Magnalium 1.0%, ethylene glycol 5%, organic silicon defoamer 0.01%, deionized water complements to 100%.It is uniformly mixed, after being sanded Obtain the plant growth regulator of the present embodiment.
The aqua of 16: one plant growth regulators of embodiment, the raw material components including following weight percent: superfine chain Lattice spore Activating protein-1 %, diethyl aminoethyl hexanoate 6%, methanol 5%, fatty alcohol polyoxyethylene ether 10%, glycerine 4%, polyethers silicon defoaming agent 0.04%, deionized water complements to 100%.The plant growth regulator of the present embodiment is obtained after mixing.
The aqua of 17: one plant growth regulators of embodiment, the raw material components including following weight percent: superfine chain Lattice spore activator protein 0.2%, zeatin 3.8%, cyclohexanone 3%, styrylphenol polyoxyethylene ether 8%, ethylene glycol 3%, Polyethers silicon defoaming agent 0.02%, deionized water complements to 100%.The plant growth regulating of the present embodiment is obtained after mixing Agent.
The aqua of 18: one plant growth regulators of embodiment, the raw material components including following weight percent: superfine chain Lattice spore activator protein 3%, compound sodium nitrophenolate 1.5%, decyl amide 2%, anhydrous sorbitol APEO 9%, propylene glycol 5% have Machine silicon defoaming agent 0.03%, deionized water complements to 100%.The plant growth regulator of the present embodiment is obtained after mixing.
Performance detection test:
1 3% toVerticilliumdahliaActivitie Activitie S of Relative activator protein wettable powder of comparative example
Raw material components including following weight percent: toVerticilliumdahliaActivitie Activitie S of Relative activator protein 3%, sodium methylene bis-naphthalene sulfonate 8%, Succinate sodium sulfonate 4%, white carbon black 5%, kaolin complements to 100%, this comparative example is obtained after being pulverized and mixed Plant growth regulator formulations.
2 8% diethyl aminoethyl hexanoate aqua of comparative example
Raw material components including following weight percent: diethyl aminoethyl hexanoate 8%, ethyl alcohol 5%, fatty alcohol polyoxyethylene ether 8%, third Triol 5%, polyethers silicon defoaming agent 0.02%, deionized water complements to 100%.The plant for obtaining this comparative example after mixing is raw It is long to adjust agent formulation.
3 5% zeatin aqua of comparative example
Raw material components including following weight percent: zeatin 5%, isopropanol 3%, styrylphenol polyoxyethylene Ether 10%, ethylene glycol 4%, polyethers silicon defoaming agent 0.02%, deionized water complements to 100%.This comparison is obtained after mixing The plant growth regulator formulations of example.
4 1.8% compound sodium nitrophenolate aqua of comparative example
Raw material components including following weight percent: compound sodium nitrophenolate 1.8%, decyl amide 4%, polyoxyethylene sorbitan Monooleate 10%, ethylene glycol 4%, polyethers silicon defoaming agent 0.02%, deionized water complements to 100%.After mixing Obtain the plant growth regulator formulations of this comparative example.
5 6.1% toVerticilliumdahliaActivitie Activitie S of Relative activator protein diethyl aminoethyl hexanoate aqua (1:60) of comparative example
Raw material components including following weight percent: toVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.1%, diethyl aminoethyl hexanoate 6.0%, wooden Plain sodium sulfonate 8%, sldium lauryl sulfate 4%, kaolin complement to 100%, and the plant of this comparative example is obtained after being pulverized and mixed Growth regulator.
6 5.04% toVerticilliumdahliaActivitie Activitie S of Relative activator protein zeatin wettable powder (1:0.0008) of comparative example
Raw material components including following weight percent: alternaria tenuis activator protein 5.0%, zeatin 0.004%, methylene The double sodium naphthalene sulfonates 8% of base, sldium lauryl sulfate 3%, attapulgite complements to 100%, this comparative example is obtained after being pulverized and mixed Plant growth regulator.
7 3.75% toVerticilliumdahliaActivitie Activitie S of Relative activator protein compound sodium nitrophenolate wettable powder (1:74) of comparative example
Raw material components including following weight percent: toVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.05%, compound sodium nitrophenolate 3.7%, Polycarboxylate 4%, pull open powder 4%, starch complements to 100%, and the plant growth regulating of this comparative example is obtained after being pulverized and mixed Agent.
Application Example 1: 1~18 plant growth regulator of embodiment is to peanut yield increasing effect measuring
Test site is located at Hebei province Langfang City Guangyang District Chinese Academy of Agricultural Sciences Langfang scientific research Pilot Base, tests plot Flat neat, soil property is sandy loam, and middle fertility is on the upper side.Reagent agent is mentioned by Institute of Plant Protection, the Chinese Academy of Agricultural Sciences Langfang pesticide pilot plant For.Peanut varieties are Ji oil 4, and mulch sowing on May 11 (machine sowing) in 2017 harvests on August 26, and preceding crop is corn. When sowing, medicament is diluted to respectively with water and is placed in and dries in the air shady and coolly after different disposal soaks seed 4h using concentration accordingly Dry, sowing is in experimental plot after drying.Seeding row spacing 12cm × 50cm.It is control, emergence with clear water and corresponding growth regulator It is each 1 time spraying in early flowering season, knit stitch phase and fruiting period afterwards, at most by spraying 3 times.Period, unified fertilizer and water management.Every processing repeats 4 It is secondary.It is arranged using random district's groups, section stays protection to go.Using parallel lines accidental sampling, every cell investigates 5 clumps totally 10 plants.? Survey production is carried out when harvesting peanut, the full pod number of measurement plant height, single plant, single plant are satisfied, and pod is heavy, satisfy per acre pod yield and seed benevolence yield.As a result It is shown in Table 1.
Effect of 1 plant growth regulator of table to peanut growth
As known from Table 1, plant growth regulator of the invention has obviously due to its distinctive ingredient and combination Synergistic effect.Spraying treatment of the peanut after seed soaking and (or) emergence 3 times, the plant growth regulator treatment and exclusive use are each Growth regulator is compared, when the use of concentration being 5~20ppm, i.e., combined when concentration is less than or equal to the dosage of corresponding single dose The full pod number of the processed peanut plant height of object, single plant, pod weight, Pod Yield and seed benevolence yield are above each plant growth regulator Single dose processing shows that the plant growth regulator has synergistic effect to the growth of peanut and volume increase.
On the basis of increasing production measurement, the present invention has also carried out plant chlorophyll content and peanut to the peanut of different disposal The measurement of quality (fat and protein content).The specific method is as follows:
1, chlorophyll content the measurement of chlorophyll content: is measured using ethyl alcohol, acetone, water mixed liquid extraction.Every cell Select 10 plant, take lower 3rd leaf in top, shred, take 0.5g be put into 25mL mixed extract (ethyl alcohol: acetone: distilled water=4.5: 4.5:1, volume ratio) in, soak extraction is until leaf fragment becomes white completely under dark.It is control with extracting solution, takes leaching Extract measures the absorbance at 645 and 663nm wavelength respectively on Shimadzu UV-1800 spectrophotometer, finally calculates chlorophyll Content.
2, quality determination: after harvest, each cell randomly selects the full dried flower without germination, without breakage, disease-free spot of 100g Non-hibernating eggs carries out fat content and crude protein content measurement.Determination of fat soxhlet extraction, crude protein measurement are micro- with half Measure Kjeldahl nitrogen determination nitrogen pool.Concrete outcome is shown in Table 2.
Influence of 2 plant growth regulator of table to peanut plant chlorophyll and peanut quality
As known from Table 2, plant growth regulator of the invention is green to peanut plant leaf due to its distinctive ingredient and combination Cellulose content has obviously increasing action.Meanwhile it being handled 3 times through root dipping processing and (or) foliar spray, the plant growth Regulator processing is compared with being used alone each growth regulator, when being less than or equal to the dosage of corresponding single dose using concentration, warp The peanut quality that compositions-treated is crossed also has large effect, and fat content and protein content have apparent increase.Show The plant growth regulator, which promotes the chlorophyll content and peanut quality of peanut, has synergistic effect.
Application Example 2: 1~18 plant growth regulator of embodiment is to increasing production of rice effect measuring
Test carries out in Jiangxi Province, the village Shang Tian, Ji'an City Taihe County.Experimental field soil is to stick loam, middle fertility, pH 5~ 6, irrigation conditions is good, experimental field long-term rice cultivation.
Reagent agent is provided by Institute of Plant Protection, the Chinese Academy of Agricultural Sciences Langfang pesticide pilot plant.Varieties of plant be " safe excellent 398 ", 2016 Sowing on June 27, in, the transplanting of 4.5~5.0 leaves harvested October 10.When sowing, medicament is diluted to respectively with water and is answered accordingly It is placed in and is dried shady and coolly, sowed after drying after different disposal seed soaking for 24 hours with concentration, seeding row spacing 18cm × 18cm.With clear Water and corresponding growth regulator single dose are control.Can respectively 7 days after transplanting, tillering stage and boot stage three phases it is each spraying 1 time, at most by spraying 3 times.The relatively uniform paddy field of growth tendency is chosen, referring to " the pesticide field GB/T17980.140-2004 The 140th part of pharmacodynamic test criterion (two): rice growth regulator test " and design concentration tested, every processing repeatedly 4 It is secondary, every plot area 20m2, have 500~550 plants of rice plant, arranged using random district's groups, between great Qu between cell Equipped with isolation strip, independent row irrigation is set, even spraying application is carried out to cell using MATABI-16 type sprayer.Period, system One fertilizer and water management.
Control time and method: plant height investigation: harvest time is investigated 1 time, 5 points of every cell samplings, 3 clumps of rice of every investigation, Totally 15 clumps, record plant height.
Tillering capacity investigation: being investigated in rice tillering latter stage, and 5 points of every cell samplings, every solid point, which is seted the tone, looks into 3 clumps of rice, It totally 15 clumps, calculates and increases tiller rate.
Yield investigation: (1) every total grain panicle number.3 clumps of rice of every cell random searching investigate total grain number of every fringe, calculate flat Mean value.(2) mass of 1000 kernel.Every cell random searching thousand corn weight, is repeated 3 times, and calculates average value.(3) in harvest time to each cell It carries out paid survey to produce, converts yield.It the results are shown in Table 3.
The effect that 3 plant growth regulator of table promotes paddy growth
As known from Table 3, plant growth regulator of the invention has obviously due to its distinctive ingredient and combination Synergistic effect.Spraying treatment of the rice after seed soaking and (or) emergence 2~3 times, the plant growth regulator treatment with individually make It is compared with each growth regulator, when the use of concentration being 3~20ppm, i.e., when concentration is less than or equal to the dosage of corresponding single dose, warp Plant Height of Rice, tiller number, every total grain panicle number, mass of 1000 kernel, cell production and the equivalent per mu yield that compositions-treated is crossed are above respectively The single dose of plant growth regulator is handled, and shows that the plant growth regulator has synergistic effect to the growth of rice and volume increase.
On the basis of increasing production measurement, the present invention has also carried out plant chlorophyll content and rice to the rice of different disposal The measurement of quality (polished rice rate, head rice rate, chalkness ratio, amylose content and protein content).The specific method is as follows:
1, chlorophyll content the measurement of chlorophyll content: is measured using ethyl alcohol, acetone, water mixed liquid extraction.Last 7 days after secondary application, take blade in the middle part of boot leaf, shred, take 0.1g be put into 20mL mixed extract (ethyl alcohol: acetone: distilled water= 4.5:4.5:1, volume ratio) in, soak extraction is until leaf fragment becomes white completely under dark.It is control with extracting solution, It takes leaching liquor to measure the absorbance at 645 and 663nm wavelength respectively on Shimadzu UV-1800 spectrophotometer, finally calculates leaf Chlorophyll contents.
2, quality determination: paddy uses rice polisher husk rice after drying, and measures polished rice rate, whole essence by standard NY 147-88 method Rice rate and chalkness ratio.Amylose content is measured by standard NY 147-88 method, and protein content is surveyed using Kjeldahl's method It is fixed.Concrete outcome is shown in Table 4.
Influence of 4 plant growth regulator of table to rice plant chlorophyll and rice quality
As known from Table 4, plant growth regulator of the invention is green to rice plant leaf due to its distinctive ingredient and combination Cellulose content has obviously increasing action.Meanwhile after seed soaking and (or) foliar spray processing, the plant growth tune Agent processing is saved compared with each growth regulator is used alone, when being less than or equal to the dosage of corresponding single dose using concentration, through group Closing the processed rice quality of object also has large effect, and polished rice rate, head rice rate and protein content have apparent increase, Chalkness ratio, amylose content reduce to a certain degree.Show the plant growth regulator to the chlorophyll content and rice of rice Quality-improving has synergistic effect.
Application Example 3: 1~18 plant growth regulator of embodiment measures Apple Growth facilitation effect
It tests and is carried out in the apple orchard in Mentougou, Beijing City area, the village Huang Antuo, clear water town.Trial zone fertilizer and water condition is medium.For Varieties be state's light, the age of tree 10 years.
Reagent agent is provided by Institute of Plant Protection, the Chinese Academy of Agricultural Sciences Langfang pesticide pilot plant.Use the plant growth tune of suitable concentration Section agent is sprayed, and is control with clear water and corresponding growth regulator single dose.Respectively in the early flowering season, bear fruit after and Fruit Phase three phases are each 1 time spraying, and at most by spraying 3 times.4 repetitions of every processing, cell random alignment, every cell 2 trees, every processing 8 trees.Test is sprayed with JactoHD400 type sprayer.
Investigation method: each cell randomly chooses 1 apple tree, investigates by 4 orientation calibration side shoots, each side shoot is at least There are 25 inflorescences, investigates fruit-setting rate.
Every cell randomly chooses 1 tree, chooses sampling point by 4 orientation.Every takes 10 leaves on branch, and each tree takes altogether Then 40 leaves take the circular blade of same size to weigh fresh weight on every leaf with punch, and weigh and do after it is air-dried Weight.The fruit picking later period weighs single fruit weight.It the results are shown in Table 5.
The effect that 5 plant growth regulator of table promotes Apple Growth
As known from Table 5, plant growth regulator of the invention has obviously due to its distinctive ingredient and combination Synergistic effect.Apple tree through spraying treatment 3 times, the plant growth regulator treatment compared with each growth regulator is used alone, When the use of concentration being 5~20ppm, i.e., when concentration is equal to or less than the dosage of corresponding single dose, the combined processed apple of object Tree fruit-setting rate, fresh weight, leaf dry weight and single fruit weight are above the single dose processing of each plant growth regulator, show the plant Object growth regulator has synergistic effect to the growth and volume increase of apple tree.
In above-mentioned experimental basis, the present invention has also carried out plant chlorophyll content and fruit to the apple tree of different disposal The measurement of quality (soluble solid, total Soluble Sugar and titratable acid content).The specific method is as follows:
1, chlorophyll content the measurement of chlorophyll content: is measured using ethyl alcohol, acetone, water mixed liquid extraction.It takes each small Area chooses 10 plants of apple tree, and the random picking fresh adult blade of every plant of tree shreds, 0.1g is taken to be put into 20mL mixed extract (second Alcohol: acetone: distilled water=4.5:4.5:1, volume ratio) in, soak extraction is until leaf fragment becomes white completely under dark. It is control with extracting solution, leaching liquor is taken to measure the suction at 645 and 663nm wavelength respectively on Shimadzu UV-1800 spectrophotometer Luminosity finally calculates chlorophyll content.
2, quality determination: when harvest, each cell randomly selects 10 fruits, and it is total to carry out soluble solid, solubility Sugar and acidity assaying.Soluble solid uses the " measurement of NY/T 2637-2014 fruits and vegetables soluble solid content Refractometer method " it measures, total Soluble Sugar is measured with Phenol sulfuric acid procedure, and titratable acid refers to " GB/T 12293-1990 water fruit and vegetable The measurement of dish product titrable acidity " it carries out.
It the results are shown in Table 6.
Influence of 6 plant growth regulator of table to apple plants chlorophyll and fruit quality
As known from Table 6, plant growth regulator of the invention is green to apple plants leaf due to its distinctive ingredient and combination Cellulose content has obviously increasing action.Meanwhile it being handled 3 times through foliar spray, the plant growth regulator treatment and list It is solely compared using each growth regulator, when being less than or equal to the dosage of corresponding single dose using concentration, combined object is processed Apple quality also has large effect, and soluble solid, total Soluble Sugar have apparent increase, and titratable acid content is obvious It reduces.Show that the plant growth regulator improves with synergistic effect the chlorophyll content and fruit quality of apple.
Application Example 4: 1~18 Plant Growth Regulators on Tomato growth-promoting effect of embodiment measurement
This test is located at the Shijiazhuang City of Hebei Province Xingtang County village Dong Si, and soil is sand, and the content of organic matter is 1.06%, pH value 6.8.Nursery on March 10 in 2016, field planting on April 28, seeding row spacing are 80 × 40cm.During tomato growth into Row weeding, fertilising and the prevention and control of plant diseases, pest control are managed with conventional.
Reagent agent is provided by Institute of Plant Protection, the Chinese Academy of Agricultural Sciences Langfang pesticide pilot plant.Use the plant growth tune of suitable concentration Section agent is sprayed, and is control with clear water and corresponding growth regulator single dose.The root dipping in transplanting respectively, Seedling Stage, just flower Phase and three phases are each 1 time spraying after bearing fruit, at most by spraying 3 times.4 repetitions of every processing, cell random alignment, plot area are 20m2If protection row.
Investigation method: (1) 20 days plant heights after medicine, every cell are taken 5 point samplings, are recorded after every two plants, 20 days of selection Plant plant height.(2) fruit-setting rate is investigated.Listing mark plant is pinpointed, every plant of bulk registration of florescence is bloomed sum, accumulative note before harvesting Record number of bearing fruit.Fruit-setting rate (%)=(sum of count/blooming of bearing fruit) × 100.(3) single fruit weight and determination of yield.According to field efficacy Each cell selects 10 fruits at random when test rule GB/T 17980.142-2004 is with primary harvest, weighs single fruit weight, harvest Phase, accumulative survey produced, recording cell yield.It the results are shown in Table 7.
The effect that the growth of 7 Plant Growth Regulators on Tomato of table promotes
As known from Table 7, plant growth regulator of the invention has obviously due to its distinctive ingredient and combination Synergistic effect.Tomato is handled through root dipping and (or) foliar spray is handled 3 times, and the plant growth regulator treatment and exclusive use are each Growth regulator is compared, when being less than or equal to the dosage of corresponding single dose using concentration, the processed tomato plant height of combined object, Fruit-setting rate, single fruit weight and cell production are above the single dose processing of each plant growth regulator, show the plant growth regulating Agent has synergistic effect to the growth and volume increase of tomato.
On the basis of increasing production measurement, the present invention has also carried out plant chlorophyll content and fruit to the tomato of different disposal The measurement of quality (soluble solid, total Soluble Sugar and Vitamin C content).The specific method is as follows:
1, chlorophyll content the measurement of chlorophyll content: is measured using ethyl alcohol, acetone, water mixed liquid extraction.In tomato 1st fringe fructescence takes adjacent 1st leaf in fruit lower part of plant in the middle part of each cell, shreds, and 0.1g is taken to be put into 20mL mixing In extracting solution (ethyl alcohol: acetone: distilled water=4.5:4.5:1, volume ratio), soak extraction to leaf fragment becomes completely under dark Until white.It is control with extracting solution, leaching liquor is taken to measure 645 and 663nm wave respectively on Shimadzu UV-1800 spectrophotometer The absorbance of strong point, finally calculates chlorophyll content.
2, quality determination: when harvest, each cell randomly selects 10 fruits, and it is total to carry out soluble solid, solubility Sugar, acidity and Vitamin C content measurement.Soluble solid uses " NY/T 2637-2014 fruits and vegetables solubility solid The measurement refractometer method of object content " it measures, total Soluble Sugar is measured with Phenol sulfuric acid procedure, and titratable acid refers to " GB/T 12293- The measurement of 1990 fruit, vegetable product titrable acidity " it carries out, vitamin C is using " anti-bad in GB 5009.86-2016 food The measurement of hematic acid " it carries out.
It the results are shown in Table 8.
The influence of 8 Plant Growth Regulators on Tomato plant chlorophyll of table and fruit quality
As known from Table 8, plant growth regulator of the invention is green to tomato plant leaf due to its distinctive ingredient and combination Cellulose content has obviously increasing action.Meanwhile it being handled 3 times through root dipping processing and (or) foliar spray, the plant growth Regulator processing is compared with being used alone each growth regulator, when being less than or equal to the dosage of corresponding single dose using concentration, warp The Tomato Quality that compositions-treated is crossed also has large effect, and soluble solid, total Soluble Sugar and vitamin C have obviously Increase, titratable acid content is substantially reduced.Show the chlorophyll content and fruit of the Plant Growth Regulators on Tomato plant Quality improving has synergistic effect.
By above-mentioned 4 Application Examples it is found that plant growth regulator provided by the invention is activated using toVerticilliumdahliaActivitie Activitie S of Relative Albumen and diethyl aminoethyl hexanoate, zeatin, compound sodium nitrophenolate any one mutual compatibility, after compounding, toVerticilliumdahliaActivitie Activitie S of Relative activator protein can be very big The other several substances of promotion absorption, it is maximized to absorb and utilize each plant growth regulators, jointly in plant It plays a role, to play synergistic effect, and effectively improves crop yield.By compounding after in the use concentration of restriction and use Method, which is directed under the various crops such as rice, corn, wheat, peanut, cotton, vegetables, tobacco, fruit tree, gardens, flowers, has synergy Effect, increasing both production and income, and there is apparent effect of gain for the fruit of fruit tree.It is remarkably improved crop chlorophyll content, is mentioned High quality of agricultural product.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above Detail a variety of simple deformations can be carried out to technical solution of the present invention within the scope of the technical concept of the present invention, this A little simple deformations all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally The thought of invention, it should also be regarded as the disclosure of the present invention.

Claims (10)

1. a plant growth regulators, it is characterised in that: its effective component include toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, Any one in zeatin, compound sodium nitrophenolate.
2. plant growth regulator according to claim 1, it is characterised in that: the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and amine Fresh ester, zeatin, compound sodium nitrophenolate weight ratio be respectively 1:(0.0005~50), 1:(0.001~100) and 1:(0.0001~ 50)。
3. plant growth regulator according to claim 2, it is characterised in that: the toVerticilliumdahliaActivitie Activitie S of Relative activator protein and amine Fresh ester, zeatin, compound sodium nitrophenolate weight ratio be respectively 1:(0.001~40), 1:(0.01~50) and 1:(0.005~40).
4. plant growth regulator according to claim 1, it is characterised in that: the effective component accounts for the plant growth The 0.01~90% of regulator gross mass.
5. plant growth regulator according to any one of claims 1 to 4, it is characterised in that: the plant growth tune The dosage form for saving agent is any one of wettable powder, water dispersible granules, suspending agent and aqua.
6. the wettable powder of a plant growth regulators, it is characterised in that: the wettable powder includes following weight hundred The raw material components of score: 0.01%~80% effective component, 1%~15% dispersing agent, 1%~6% wetting agent, filler are mended Enough to 100%;The effective component include weight ratio be 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1:(0.0001~50) toVerticilliumdahliaActivitie Activitie S of Relative activate egg One of white and compound sodium nitrophenolate;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, calcium dodecyl benzene sulfonate Any one or more of with polycarboxylate;The wetting agent includes Succinate sodium sulfonate, sldium lauryl sulfate and two isobutyls Any one or more of base sodium naphthalene sulfonate pull open powder;The filler includes kaolin, diatomite, attapulgite, montmorillonite, swollen Moisten any one or more of soil, clay, talcum powder, white carbon black, calcium carbonate and starch.
7. the water dispersible granules of a plant growth regulators, it is characterised in that: the water dispersible granules include following weight hundred The raw material components of score: 0.01%~80% effective component, 5%~15% dispersing agent, 2%~8% wetting agent, 0~ 5% disintegrating agent, 0~5% binder, filler complement to 100%;The effective component includes that weight ratio is 1:(0.0005 ~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin Or 1:(0.0001~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and one of compound sodium nitrophenolate;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, calcium dodecyl benzene sulfonate Any one or more of with polycarboxylate, wetting agent includes Succinate sodium sulfonate, sldium lauryl sulfate and diisobutyl naphthalene Any one or more of sulfonic acid;The disintegrating agent includes urea, ammonium sulfate, sodium chloride, sodium carbonate, appointing in sodium alginate It is one or more of;The binder includes any one of sucrose, glucose, maltose, dextrin, polyvinyl alcohol and polyethylene glycol Or it is several;The inert solid carrier include kaolin, diatomite, attapulgite, montmorillonite, bentonite, clay, talcum powder, Any one or more of white carbon black, calcium carbonate and starch.
8. the suspending agent of a plant growth regulators, it is characterised in that: the suspending agent includes the original of following weight percent Expect component: 0.01%~50% effective component, 2%~8% dispersing agent, 1%~4% wetting agent, 0~2% thickening Agent, 0~5% antifreezing agent, 0.01~0.05% defoaming agent, deionized water complement to 100%;The effective component includes weight Amount than be 1:(0.0005~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative swash Living protein and zeatin or 1:(0.0001~50) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and one of compound sodium nitrophenolate;
The dispersing agent includes sodium lignin sulfonate, calcium lignosulfonate, sodium methylene bis-naphthalene sulfonate, calcium dodecyl benzene sulfonate Any one or more of with polycarboxylate;The wetting agent includes Succinate sodium sulfonate, sldium lauryl sulfate, diisobutyl Any one or more of sodium naphthalene sulfonate and alkyl phenol polyoxyethylene ether;The thickener includes xanthan gum, aluminium-magnesium silicate, carboxylic first In base sodium cellulosate, hydroxyethyl cellulose, gelatin, Arabic gum, polyvinyl alcohol, polyvinylpyrrolidone and polyvinyl acetate It is any one or several;The antifreezing agent includes any in ethylene glycol, propylene glycol, glycerine, urea, sodium chloride and calcium chloride Kind is several;The defoaming agent includes polyether antifoam agent and/or organic silicon defoamer.
9. the aqua of a plant growth regulators, it is characterised in that: the aqua includes the raw material group of following weight percent Point: 0.01%~50% effective component, cosolvent 0~5%, emulsifier 1~15%, antifreezing agent 0~5%, defoaming agent 0.01 ~0.05%, deionized water complements to 100%;The effective component include weight ratio be 1:(0.0005~50) superfine chain lattice Spore activator protein and diethyl aminoethyl hexanoate, 1:(0.001~100) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and zeatin or 1:(0.0001~50) ToVerticilliumdahliaActivitie Activitie S of Relative activator protein and one of compound sodium nitrophenolate;
The cosolvent includes toluene, dimethylbenzene, solvent naphtha, methanol, ethyl alcohol, isopropanol, acetone, acetonitrile, cyclohexanone, N- methyl Any one or more of pyrrolidones and decyl amide;The emulsifier includes anhydrous sorbitol APEO, poly alkyl alcohol Ethylene oxide ether, polyoxyethylene sorbitan ether monoleate, styrylphenol polyoxyethylene ether, dibenzyl phenol polyethenoxy Any one or more of ether, aliphatic amine polyoxyethylene ether and calcium dodecyl benzene sulfonate;The antifreezing agent includes ethylene glycol, third Any one or more of glycol, glycerine, urea, sodium chloride, calcium chloride;The defoaming agent include polyether antifoam agent and/or Organic silicon defoamer.
10. a kind of method of administration of the described in any item plant growth regulator of Claims 1 to 5, it is characterised in that: the plant The application concentration of object growth regulator is 1.0~20ppm, is applied using seed dressing, seed soaking, root dipping, pouring root or by the way of spraying, or Person uses to be sprayed 2~3 times in the plant growth critical period simultaneously in seed dressing, seed soaking, root dipping or pouring root.
CN201811522115.1A 2018-04-01 2018-12-13 One plant growth regulators and its method of administration Pending CN109601537A (en)

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CN110122482A (en) * 2019-05-08 2019-08-16 北京普绿通生物肥料厂 A kind of preparation process of toVerticilliumdahliaActivitie Activitie S of Relative PeaT1 plant immune protein soluble powder
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CN110205248A (en) * 2019-06-12 2019-09-06 中国矿业大学(北京) A kind of method of simultaneous inoculation AM and the promotion plant growth of DSE fungi and its microbial bacterial agent used
CN110999904A (en) * 2019-11-13 2020-04-14 浙江工业大学 Protein exciton PeaT1 slow controlled release agent and preparation and application thereof
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CN111848240A (en) * 2020-08-03 2020-10-30 山东中创亿丰肥料集团有限公司 Microbial agent capable of promoting flower bud differentiation of plants
CN113133459A (en) * 2021-04-20 2021-07-20 河南省莱恩坪安园林植保有限公司 Special growth regulator for plant infusion
CN113149759A (en) * 2021-04-23 2021-07-23 中国农业科学院油料作物研究所 Crop florescence prolonging agent and preparation method thereof
CN117204427A (en) * 2023-11-08 2023-12-12 西北农林科技大学深圳研究院 Agricultural composition for apple flower thinning and fruit thinning
CN117204427B (en) * 2023-11-08 2024-02-23 西北农林科技大学深圳研究院 Agricultural composition for apple flower thinning and fruit thinning
CN118126846A (en) * 2024-05-06 2024-06-04 云南省林业和草原科学院 Culture method and application of pseudogold Mao Nasheng fungus strain

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Application publication date: 20190412