CN109588711B - Fucoxanthin-oyster peptide nanoparticles and preparation method and application thereof - Google Patents

Fucoxanthin-oyster peptide nanoparticles and preparation method and application thereof Download PDF

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CN109588711B
CN109588711B CN201910073381.9A CN201910073381A CN109588711B CN 109588711 B CN109588711 B CN 109588711B CN 201910073381 A CN201910073381 A CN 201910073381A CN 109588711 B CN109588711 B CN 109588711B
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fucoxanthin
oyster peptide
oyster
solution
fatty acid
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CN109588711A (en
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刘翼翔
傅秋叶
刘光明
孙乐常
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Jimei University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/20—Agglomerating; Granulating; Tabletting
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention belongs to the field of foods, and particularly relates to a fucoxanthin-oyster peptide nanoparticle as well as a preparation method and application thereof. The preparation method of the fucoxanthin-oyster peptide nanoparticles comprises the following steps: (1) Dissolving the oyster peptide by water, and adjusting the pH value to 10-12 to obtain alkaline oyster peptide aqueous solution; (2) Dissolving fucoxanthin powder with sodium hydroxide ethanol solution, adding fatty acid, and mixing to obtain fucoxanthin-fatty acid dispersion; (3) Uniformly mixing an alkaline oyster peptide aqueous solution and a fucoxanthin-fatty acid dispersion liquid, and performing ultrasonic treatment; (4) Adjusting the pH value of the solution obtained in the step (3) to 6-8 to obtain a clear fucoxanthin-oyster peptide compound water solution; (5) spray drying or freeze drying. The fucoxanthin-oyster peptide nanoparticles prepared by the method have the advantages of microcapsules and nano emulsion, and have the characteristics of good water solubility, strong stability, high absorptivity, simple process and no chemical residue.

Description

Fucoxanthin-oyster peptide nanoparticles and preparation method and application thereof
Technical Field
The invention belongs to the field of foods, and particularly relates to a fucoxanthin-oyster peptide nanoparticle as well as a preparation method and an application thereof.
Background
Fucoxanthin is xanthophylls in brown algae, and has various biological activities such as eyesight protection, liver protection, lipid metabolism regulation, etc. However, the unique long-chain conjugated structure (containing allene structure) causes the fucoxanthin to have poor stability, is extremely easy to be damaged by conditions such as high temperature, oxygen, illumination and the like, and is difficult to be applied to food production; in addition, the fat-soluble characteristic of the fucoxanthin makes the fucoxanthin difficult to be uniformly dispersed in a hydrophilic system of food, thereby causing multiple barriers for human body absorption and being difficult to exert the unique physiological activity. Therefore, the technical key for implementing the industrial development of the fucoxanthin is to improve the stability of the fucoxanthin and promote the absorption and utilization of the fucoxanthin by human bodies.
The conventional microcapsule embedding technology generally emphasizes the improvement of the carotenoid encapsulation capacity, solubility and stability, neglects the absorption effect of a human body and breaks against the original purpose of nutrition enhancement. For example, the β -carotene water-dispersible dry powder produced by the Xinchang pharmaceutical factory, zhejiang, 10% CWS and 1% β -carotene emulsion can be used directly for coloring and fortifying beverages, liquid foods, etc. However, there are still a number of deficiencies with current carotenoid formulations, mainly manifested as: (1) potential safety hazards exist, and organic solvents remain in products; (2) The carotenoid micro-encapsulation preparation has the particle size of 0.7 to 5 mu m; (3) The production efficiency is low, and the carotenoid is lost more in the production process. In order to make up for the deficiency of the microcapsule technology, in recent years, researchers at home and abroad begin to embed carotenoids by using micro/nano emulsion and biomacromolecules, such as self-emulsifying carriers, nano particles, nano-emulsion composite systems and the like. The Masjian subject group of the south Jiangsu university takes food biomacromolecule octenyl succinate starch as an emulsifier to construct oil-in-water (O/W) type beta-carotene nanoemulsion; salvia-Trujillo et al (2013) use corn oil-Tween 20 as a beta-carotene carrier; tian et al (2014) adopt lecithin-chitosan as a carrier to prepare the lutein nano-liposome, thereby effectively improving the processing stability of lutein; the Huang research team (2010) also inserts octanoyl and polyethylene glycol monomethyl ether groups into the molecular structure of chitosan to obtain modified chitosan, and the modified chitosan is used for carrying carotenoid so as to improve the bioavailability of the carotenoid. In addition, the kaffir-type tablet of chinese agricultural university also prepared beta-carotene nanoemulsion based on Tween series of surfactants by a high-pressure homogenization method (Yuan et al, 2008, qian et al, 2012. However, these technologies neglect the stability, safety and economy of the production process of the product while pursuing high loading and bioavailability of carotenoids. For example, the hemolysis factor can reach 60% when the Tween80 concentration (less than 100 nm) reaches 4 mg/ml (Lee et al, 2003 gong et al, 2009), which is also much lower than the typical concentration of Tween80 in nanoemulsion formulations (10-50 mg/ml); and the high-pressure homogenization and other technologies have higher requirements on equipment and higher production cost.
Disclosure of Invention
Aiming at the problems of poor processing adaptability and large application limitation caused by the fact that fucoxanthin is insoluble in water, poor in stability and low in absorptivity, the invention provides the fucoxanthin-oyster peptide nanoparticles which are good in water solubility, strong in stability and high in absorptivity, and the preparation method and the application thereof through improving the existing processing method.
Specifically, the invention provides a preparation method of fucoxanthin-oyster peptide nanoparticles, which comprises the following steps:
(1) Dissolving the oyster peptide by adopting water, adjusting the pH value to 10-12 to open a hydrophobic inner cavity of the oyster peptide to obtain alkaline oyster peptide aqueous solution;
(2) Dissolving fucoxanthin powder with sodium hydroxide ethanol solution, adding fatty acid, and mixing to obtain fucoxanthin-fatty acid dispersion;
(3) Uniformly mixing the alkaline oyster peptide aqueous solution and the fucoxanthin-fatty acid dispersion liquid, and performing ultrasonic treatment to fully combine fucoxanthin and oyster peptide hydrophobic cavities;
(4) Adjusting the pH value of the solution obtained in the step (3) to 6-8 to seal the oyster peptide inner cavity to obtain a clear fucoxanthin-oyster peptide compound water solution;
(5) And directly spray-drying the fucoxanthin-oyster peptide compound aqueous solution, or freeze-drying after decompression concentration to obtain fucoxanthin-oyster peptide nanoparticle dry powder.
Preferably, in the step (1), the molecular weight of the oyster peptide is 1000-5000 Da.
Preferably, in the step (1), the oyster peptide and water are used in such amounts that the concentration of the obtained oyster peptide solution is 0.5 to 8wt%.
Preferably, in the step (2), the concentration of the sodium hydroxide in the sodium hydroxide ethanol solution is 0.1-1 wt%.
Preferably, in the step (2), the dosage ratio of the fucoxanthin to the sodium hydroxide ethanol solution is 1g (100-300) mu L.
Preferably, in the step (2), the concentration of fucoxanthin in the fucoxanthin-fatty acid dispersion liquid is 30wt%.
Preferably, in the step (2), the fatty acid is an unsaturated fatty acid which has the characteristics of small molecular volume and flexible structure. The unsaturated fatty acid is preferably at least one selected from the group consisting of oleic acid, linoleic acid, linolenic acid, arachidonic acid, and docosahexaenoic acid.
Preferably, in the step (3), the mass ratio of the fucoxanthin-fatty acid dispersion to the basic oyster peptide aqueous solution calculated on the basis of the oyster peptide is 20.
Preferably, in the step (3), the time of the ultrasonic treatment is 0.5 to 2 hours.
Preferably, in the step (4), the reagent used for adjusting the pH of the solution to 6 to 8 is an acidic flavoring agent selected from at least one of acetic acid, citric acid, lactic acid, tartaric acid, malic acid and gluconolactone.
The invention also provides the fucoxanthin-oyster peptide nanoparticles prepared by the method.
Preferably, the fucoxanthin-oyster peptide nanoparticles do not flocculate or delaminate when placed at normal temperature for 12 months, the retention rate of the fucoxanthin is more than 90%, and the retention rate of the fucoxanthin is more than 90% when placed at normal temperature for 24 months.
Preferably, the average particle size of the fucoxanthin-oyster peptide nanoparticles after being dissolved in water is 50-100 nm, and the light transmittance at 660nm is more than 98%.
Preferably, the fucoxanthin-oyster peptide nanoparticles can improve the absorptivity of fucoxanthin by 4.52-6.18 times in an aqueous system.
Preferably, the embedding rate of the fucoxanthin in the fucoxanthin-oyster peptide nanoparticles is more than 85%.
The fucoxanthin-oyster peptide nanoparticle powder is convenient to store and transport, has good absorption effect and high nutritional value, and can be widely used as a nutritional enhancer in food systems such as beverages, baked foods, candies, dairy products, ice cream and the like. Correspondingly, the invention also provides application of the fucoxanthin-oyster peptide nanoparticles as a food nutrition enhancer.
After intensive research, the inventors of the present invention found that zinc element can promote the absorption efficiency of carotenoid nutrients. Therefore, the fucoxanthin-rich oyster peptide is compatible with the oyster peptide rich in organic zinc, so that the absorption and targeted delivery efficiency of the fucoxanthin can be improved. In addition, the invention further improves the eyesight protection effect of the product by taking the protein self-assembly nanotechnology as an effective means for improving the absorption efficiency and stability of the fucoxanthin.
Compared with the prior art, the invention has the beneficial effects that: the fucoxanthin-oyster peptide nanoparticles obtained by the method provided by the invention have the advantages of microcapsules and nano emulsion, and have the characteristics of strong stability, good absorption effect, simple production process and no chemical residue. The method fully utilizes the characteristics of similarity and compatibility of hydrophobic molecules and reversible change of a protein peptide structure under the condition of pH circulation, so that the embedding rate of the fucoxanthin reaches more than 85 percent; the invention selects the unsaturated fatty acid with small molecular volume and flexible structure as a medium, so that the grain diameter of the fucoxanthin-oyster peptide nano-particles is 50-100 nm which is far smaller than the grain diameter of the microcapsules, and meanwhile, the oyster peptide contains abundant zinc and selenium elements, thereby further promoting the absorption of the fucoxanthin, greatly improving the absorption efficiency of the product in a water system, and improving the absorption efficiency by 4.52-6.18 times; the prepared fucoxanthin-oyster peptide nanoparticles are powdery, so that the stability is good, the storage and the transportation are convenient, the advantages of being more stable than nano emulsion are also achieved, and the retention rate of the fucoxanthin can reach over 90 percent after the fucoxanthin-oyster peptide nanoparticles are stored for 24 months at normal temperature; the prepared fucoxanthin-oyster peptide nanoparticles have good rehydration property, can form clear and transparent liquid in aqueous solution, has light transmittance of more than 98 percent at 660nm, enlarges the application range of products, and has wide application in the fields of foods and biological medicines; the preparation method has simple route, does not need high-pressure homogenization and high-speed shearing processing, has the advantage of low production cost, and is suitable for industrial production; in addition, in the process of preparing the fucoxanthin-oyster peptide nanoparticles, the used raw materials are not limited by any addition in food, no toxic and harmful substance residue exists, the product safety is high, and the requirements of food safety are met.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative of the invention and is not to be construed as limiting the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are conventional products which are commercially available, and are not indicated by manufacturers.
Example 1
(1) Weighing 0.5g of oyster peptide with average molecular weight of 1000Da, adding 99.5mL of deionized water, fully stirring for dissolving, and adjusting the pH value to 12 by using sodium hydroxide to obtain an alkaline oyster peptide solution with the concentration of 0.5 wt%;
(2) Weighing 3.0g of fucoxanthin powder, adding 300 mu L of 1.0wt% sodium hydroxide ethanol solution for full dissolution, then adding 7.0g of oleic acid, and uniformly stirring to obtain fucoxanthin dispersion liquid with the fucoxanthin concentration of 30wt%;
(3) Slowly adding 10.0g of fucoxanthin dispersion liquid into all the alkaline oyster peptide solution obtained in the step (1), uniformly stirring, and carrying out ultrasonic treatment for 2 hours to ensure that fucoxanthin and the hydrophobic inner cavity of the oyster peptide are fully combined;
(4) Adjusting the pH value of the solution obtained in the step (3) back to 7.0 by using glucolactone to obtain a fucoxanthin-oyster peptide complex aqueous solution (the fucoxanthin embedding rate is 85%);
(5) And directly spray-drying the fucoxanthin-oyster peptide compound aqueous solution, or freeze-drying after decompression concentration to obtain fucoxanthin-oyster peptide nanoparticle dry powder.
The physicochemical properties and the absorption effect of the fucoxanthin-oyster peptide nanoparticle dry powder are inspected, and specifically: redissolving the fucoxanthin into deionized water to form a clear and transparent solution, wherein the average particle size is 100nm, the light transmittance at 660nm is 98%, the fucoxanthin cannot be flocculated or layered after being stored for 12 months at normal temperature, the retention rate of the fucoxanthin is 91.3%, and the retention rate of the fucoxanthin is 90.2% after being stored for 24 months at normal temperature; after the fucoxanthin-oyster peptide nanoparticle dry powder is dissolved in deionized water (0.2 mu mol/mL) (treatment group), the mice are gazed, fucoxanthin which is not combined with oyster peptide is used as a control group (control group), the content of the fucoxanthin in the plasma of the mice is analyzed after 4 hours, and compared with the control group (91.3 pmol/mL), the content of the fucoxanthin in the plasma of the mice in the treatment group reaches 570.6pmol/mL, which is improved by 5.25 times.
Example 2
(1) Weighing 1.5g of oyster peptide with the average molecular weight of 3000Da, adding 98.5mL of deionized water, fully stirring for dissolving, and adjusting the pH value to 11 by using sodium hydroxide to obtain an alkaline oyster peptide solution with the concentration of 1.5 wt%;
(2) Weighing 4.5g of fucoxanthin powder, adding 450 mu L of 1.0wt% sodium hydroxide ethanol solution for full dissolution, then adding 10.5g of linoleic acid, and uniformly stirring to obtain fucoxanthin dispersion liquid with the fucoxanthin concentration of 30wt%;
(3) Slowly adding 15.0g of fucoxanthin dispersion solution into all the alkaline oyster peptide solution obtained in the step (1), uniformly stirring, and carrying out ultrasonic treatment for 1 hour to ensure that fucoxanthin and the hydrophobic inner cavity of the oyster peptide are fully combined;
(4) Adjusting the pH value of the solution obtained in the step (3) back to 7.0 by using glucolactone to obtain a fucoxanthin-oyster peptide complex aqueous solution (the fucoxanthin embedding rate is 86%);
(5) And directly spray-drying the fucoxanthin-oyster peptide complex water solution, or freeze-drying after decompression concentration to obtain the fucoxanthin-oyster peptide nano-particle dry powder.
The physicochemical property and the absorption effect of the fucoxanthin-oyster peptide nanoparticle dry powder are inspected, and specifically: redissolving the fucoxanthin into deionized water to form a clear and transparent solution, wherein the average particle size is 78nm, the light transmittance at 660nm is 98%, the fucoxanthin cannot be flocculated or layered after being stored for 12 months at normal temperature, the retention rate of the fucoxanthin is 91.7%, and the retention rate of the fucoxanthin is 92.2% after being stored for 24 months at normal temperature; after the fucoxanthin-oyster peptide nanoparticle dry powder is dissolved in deionized water (0.2 mu mol/mL) (treatment group), the gastric lavage is carried out on mice, fucoxanthin which is not combined with oyster peptide is used as a control group (control group), after 4 hours, the content of the fucoxanthin in the plasma of the mice in the treatment group is analyzed, and compared with the control group (91.3 pmol/mL), the content of the fucoxanthin in the plasma of the mice in the treatment group reaches 655.5pmol/mL and is improved by 6.18 times.
Example 3
(1) Weighing 4.0g of oyster peptide with the average molecular weight of 5000Da, adding 96mL of deionized water, fully stirring for dissolving, and adjusting the pH value to 10 by using sodium hydroxide to obtain an alkaline protein solution with the concentration of 4.0 wt%;
(2) Weighing 1.2g of fucoxanthin powder, adding 240 mu L of 0.5wt% sodium hydroxide ethanol solution for full dissolution, then adding 2.8g of linolenic acid, and stirring uniformly to obtain fucoxanthin dispersion liquid with the fucoxanthin concentration of 30wt%;
(3) Slowly adding 4.0g of fucoxanthin dispersion liquid into all the alkaline protein solution obtained in the step (1), uniformly stirring, and carrying out ultrasonic treatment for 1.5 hours to ensure that fucoxanthin is fully combined with the hydrophobic inner cavity of the oyster peptide;
(4) Adjusting the pH value of the solution obtained in the step (3) back to 7.0 by using glucolactone to obtain a fucoxanthin-oyster peptide compound aqueous solution (the fucoxanthin embedding rate is 91.5%);
(5) And directly spray-drying the fucoxanthin-oyster peptide compound aqueous solution, or freeze-drying after decompression concentration to obtain fucoxanthin-oyster peptide nanoparticle dry powder.
The physicochemical properties and the absorption effect of the fucoxanthin-oyster peptide nanoparticle dry powder are inspected, and specifically: redissolving the fucoxanthin into deionized water to form a clear and transparent solution, wherein the average particle size is 100nm, the light transmittance at 660nm is 98%, the fucoxanthin cannot be flocculated or layered after being stored for 12 months at normal temperature, the retention rate of the fucoxanthin is 95.7%, and the retention rate of the fucoxanthin is 94.9% after being stored for 24 months at normal temperature; after the fucoxanthin-oyster peptide nanoparticle dry powder is dissolved in deionized water (0.2 mu mol/mL) (treatment group), the gastric lavage is carried out on mice, fucoxanthin which is not combined with oyster peptide is used as a control group (control group), the content of the fucoxanthin in the plasma of the mice is analyzed after 4 hours, and compared with the control group (91.3 pmol/mL), the content of the fucoxanthin in the plasma of the mice of the treatment group reaches 591.6pmol/mL and is increased by 5.48 times.
Example 4
(1) Weighing 8.0g of oyster peptide with the average molecular weight of 3000Da, adding 92mL of deionized water, fully stirring and dissolving, and adjusting the pH value to 11 by using sodium hydroxide to obtain an alkaline protein solution with the concentration of 8.0 wt%;
(2) Weighing 0.48g of fucoxanthin powder, adding 144 mu L of 0.1wt% sodium hydroxide ethanol solution for full dissolution, then adding 1.12g of arachidonic acid, and stirring uniformly to obtain fucoxanthin dispersion liquid with the fucoxanthin concentration of 30wt%;
(3) Slowly adding 1.6g of fucoxanthin dispersion liquid into all the alkaline protein solution obtained in the step (1), uniformly stirring, and carrying out ultrasonic treatment for 2 hours to ensure that fucoxanthin and oyster peptide are fully combined in a hydrophobic inner cavity;
(4) Adjusting the pH value of the solution obtained in the step (3) to 7.0 by using glucolactone to obtain a fucoxanthin-oyster peptide complex aqueous solution (the fucoxanthin embedding rate is 95.1%);
(5) And directly spray-drying the fucoxanthin-oyster peptide complex water solution, or freeze-drying after decompression concentration to obtain the fucoxanthin-oyster peptide nano-particle dry powder.
The physicochemical properties and the absorption effect of the fucoxanthin-oyster peptide nanoparticle dry powder are inspected, and specifically: redissolving the fucoxanthin into deionized water to form a clear and transparent solution, wherein the average particle size is 97nm, the light transmittance at 660nm is 98%, the fucoxanthin cannot be flocculated or layered after being stored for 12 months at normal temperature, the retention rate of the fucoxanthin is 92.1%, and the retention rate of the fucoxanthin is 94.6% after being stored for 24 months at normal temperature; after the fucoxanthin-oyster peptide nanoparticle dry powder is dissolved in deionized water (0.2 mu mol/mL) (a treatment group), a gastric lavage mouse is perfused, fucoxanthin which is not combined with oyster peptide is used as a control group, after 4 hours, the content of the fucoxanthin in the plasma of the mouse of the treatment group is analyzed, and compared with the control group (91.3 pmol/mL), the content of the fucoxanthin in the plasma of the mouse of the treatment group reaches 546pmol/mL and is increased by 4.98 times.
Example 5
(1) Weighing 8.0g of oyster peptide with the average molecular weight of 3000Da, adding 92mL of deionized water, fully stirring for dissolving, and adjusting the pH value to 11 by using sodium hydroxide to obtain an alkaline protein solution with the concentration of 8.0 wt%;
(2) Weighing 0.24g of fucoxanthin powder, adding 48 mu L of 0.5wt% sodium hydroxide ethanol solution for full dissolution, then adding 0.56g of docosahexaenoic acid, and stirring uniformly to obtain a fucoxanthin dispersion liquid with the fucoxanthin concentration of 30wt%;
(3) Slowly adding 0.8g of fucoxanthin dispersion liquid into all the alkaline protein solution obtained in the step (1), uniformly stirring, and carrying out ultrasonic treatment for 0.5 hour to ensure that fucoxanthin and oyster peptide hydrophobic cavities are fully combined;
(4) Adjusting the pH value of the solution obtained in the step (3) back to 7.0 by using glucolactone to obtain a fucoxanthin-oyster peptide compound aqueous solution (the fucoxanthin embedding rate is 97%);
(5) And directly spray-drying the fucoxanthin-oyster peptide complex water solution, or freeze-drying after decompression concentration to obtain the fucoxanthin-oyster peptide nano-particle dry powder.
The physicochemical properties and the absorption effect of the fucoxanthin-oyster peptide nanoparticle dry powder are inspected, and specifically: redissolving the fucoxanthin into deionized water to form a clear and transparent solution, wherein the average particle size is 100nm, the light transmittance at 660nm is 98%, the fucoxanthin cannot be flocculated or layered after being stored for 12 months at normal temperature, the retention rate of the fucoxanthin is 97.1%, and the retention rate of the fucoxanthin is 96.2% after being stored for 24 months at normal temperature; after the fucoxanthin-oyster peptide nanoparticle dry powder is dissolved in deionized water (0.2 mu mol/mL) (a treatment group), a gastric lavage mouse is gazed, fucoxanthin which is not combined with oyster peptide is used as a control group (a control group), the content of the fucoxanthin in the plasma of the mouse is analyzed after 4 hours, and compared with the control group (91.3 pmol/mL), the content of the fucoxanthin in the plasma of the mouse of the treatment group reaches 504pmol/mL, and is improved by 4.52 times.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various features described in the above embodiments may be combined in any suitable manner without departing from the scope of the invention. The invention is not described in detail in order to avoid unnecessary repetition.
In addition, any combination of the various embodiments of the present invention is also possible, and the same should be considered as the disclosure of the present invention as long as it does not depart from the spirit of the present invention.

Claims (11)

1. A preparation method of fucoxanthin-oyster peptide nanoparticles is characterized by comprising the following steps:
(1) Dissolving oyster peptide with the molecular weight of 1000-5000 Da by adopting water, adjusting the pH value to 10-12, and opening a hydrophobic inner cavity of the oyster peptide to obtain alkaline oyster peptide aqueous solution;
(2) Dissolving fucoxanthin powder by using a sodium hydroxide ethanol solution, adding fatty acid, and uniformly mixing to obtain a fucoxanthin-fatty acid dispersion liquid; the concentration of fucoxanthin in the fucoxanthin-fatty acid dispersion liquid is 30wt%;
(3) Uniformly mixing the alkaline oyster peptide aqueous solution and the fucoxanthin-fatty acid dispersion liquid, and performing ultrasonic treatment to fully combine fucoxanthin and oyster peptide hydrophobic cavities;
(4) Adjusting the pH value of the solution obtained in the step (3) to 6-8 to seal the oyster peptide inner cavity to obtain a clear fucoxanthin-oyster peptide compound water solution;
(5) And directly spray-drying the fucoxanthin-oyster peptide complex water solution, or freeze-drying after decompression concentration to obtain the fucoxanthin-oyster peptide nano-particle dry powder.
2. The method for preparing fucoxanthin-oyster peptide nanoparticles according to claim 1, wherein in the step (1), the oyster peptides and water are used in an amount such that the concentration of the oyster peptide solution is 0.5 to 8wt%.
3. The method for preparing fucoxanthin-oyster peptide nanoparticles according to claim 1 or 2, wherein in the step (2), the concentration of sodium hydroxide in the sodium hydroxide ethanol solution is 0.1 to 1wt%; the dosage ratio of the fucoxanthin to the sodium hydroxide ethanol solution is 1g (100-300) mu L; the fatty acid is an unsaturated fatty acid.
4. The method for preparing fucoxanthin-oyster peptide nanoparticles according to claim 3, wherein the fatty acid is at least one selected from the group consisting of oleic acid, linoleic acid, linolenic acid, arachidonic acid, and docosahexaenoic acid.
5. The method for preparing fucoxanthin-oyster peptide nanoparticles according to claim 1 or 2, wherein in the step (3), the mass ratio of the fucoxanthin-fatty acid dispersion to the basic oyster peptide aqueous solution in terms of oyster peptide is 20 to 1; the ultrasonic treatment time is 0.5-2 hours.
6. The method for preparing fucoxanthin-oyster peptide nanoparticles according to claim 1 or 2, wherein in the step (4), the reagent used for adjusting the pH of the obtained solution back to 6 to 8 is an acidulant selected from at least one of acetic acid, citric acid, lactic acid, tartaric acid, malic acid, and gluconolactone.
7. The fucoxanthin-oyster peptide nanoparticles prepared by the method of any one of claims 1 to 6.
8. The fucoxanthin-oyster peptide nanoparticles according to claim 7, wherein the fucoxanthin-oyster peptide nanoparticles do not flocculate or delaminate when placed at room temperature for 12 months, and have a fucoxanthin retention rate of 90% or more, and a fucoxanthin retention rate of 90% or more when placed at room temperature for 24 months; the average particle size of the fucoxanthin-oyster peptide nanoparticles after being re-dissolved in water is 50-100 nm, and the light transmittance at 660nm is more than 98%.
9. The fucoxanthin-oyster peptide nanoparticles of claim 7, wherein the fucoxanthin-oyster peptide nanoparticles can increase the absorption rate of fucoxanthin by 4.52-fold to 6.18-fold in an aqueous system.
10. The fucoxanthin-oyster peptide nanoparticles of claim 7, wherein an embedding rate of fucoxanthin in the fucoxanthin-oyster peptide nanoparticles is 85% or more.
11. Use of the fucoxanthin-oyster peptide nanoparticles according to any one of claims 7 to 10 as a dietary supplement.
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