CN109588235A - Application of the polysaccharide functionalized nano selenium in selenium-rich rice plantation - Google Patents
Application of the polysaccharide functionalized nano selenium in selenium-rich rice plantation Download PDFInfo
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Abstract
The invention discloses application of the polysaccharide functionalized nano selenium in selenium-rich rice plantation.The present invention is to spray the position of rice using polysaccharide functionalized nano selenium in selenium-rich rice different plantation period or spray the soil of rice cultivation, wherein, the polysaccharide functionalized nano selenium includes one of chitosan nano selenium, pachymaran nanometer selenium and lentinan nanometer selenium or at least two.The content of selenium in Brown Rice can be improved in polysaccharide functionalized nano selenium of the invention, is conducive to human health.Polysaccharide functionalized nano selenium of the invention can be in conjunction with the heavy metal in Brown Rice, husk and stalk, so that the content of heavy metal in rice be effectively reduced.
Description
Technical field
The present invention relates to the applications of nanometer selenium, and in particular to polysaccharide functionalized nano selenium answering in selenium-rich rice plantation
With.
Background technique
Selenium is one of micronutrient element necessary to humans and animals, and plant is the Major Foods of humans and animals intake selemium nutrition
Source.Some results of study show that rice has bioconcentration to selenium, therefore as staple food, rice selenium content and human body selenium
Nutrition condition is in close relations.Have many reports by applying selenium fertilizer raising plant Se content, also has application in production.Pass through blade face
The high inorganic selenium of abiotic activity and toxicity is converted into safety using the biological concentration and transformation of rice by Spraying Se-fertilizer
And the active organic selenium that toxicity is low, biological effectiveness is high, it is to improve and meet in food chain the horizontal insufficient low cost of selenium and can
Capable method.The binding force of selenium and metal is very strong, moreover it is possible to antagonism and the poison for weakening the heavy metal elements such as cadmium, arsenic, mercury, chromium in body
Property, the alleviation mechanism of heavy metal in agricultural product is also started to attract attention.
Currently, being the selenium mineral powder of selenite and activation using most external source selenium.Natural mode divalent selenium and zeroth order selenium are equal
Extremely difficult to be dissolved in water, toxicity is low, but also almost inactive；It is maximally efficient with selenite soluble easily in water that crop applies selenium,
But it is toxic.The selenite of low concentration and the selenium mineral powder of activation are absorbed and utilized that conversion ratio is low, high concentration toxicity is high, as battalion
Element is supported, the dosage of selenium is narrow using safe range, and effective usage amount is difficult to control.
The application that nano material reaches its maturity in biology triggers the development of a new technology --- nano red list
Matter selenium.Nanometer selenium is the red selenium simple substance that a kind of partial size is in Nano grade.This nanometer selenium is to thermostabilization, nontoxic, biological work
Property it is high.The biological utilisation of nanometer selenium is equal with selenomethionine, can be utilized by body efficient absorption.Selenomethionine is closed in various selenizings
There is optimal biological utilisation in object.Nanometer selenium and selenomethionine are to mouse tissue glutathione peroxidase and sulphur oxygen also egg
The vigor of white reductase has identical mediating effect+6, this is different from the elemental selenium of general zero-valent state.Under nutrition dosage, selenium egg
The difference that propylhomoserin and nanometer selenium are accumulated in whole blood, liver and renal tissue selenium is smaller；And under super nutrition dosage, the difference of two kinds of selenium
It is extremely not significant.However, the strong accumulation of selenomethionine in the tissue may cause virulent property, and accumulation is also likely to be its poison by force
One of property mechanism.2000 Nian Gaoxue clouds etc. research shows that: nanometer selenium and sodium selenite utilization rate difference in Mice Body are little, but
Sodium selenite urgency toxicity is about 7 times of nanometer selenium.Nanometer selenium partial size made from general inorganic synthetic method method is big, and stability is poor.
More mature preparation route mainly with various Template synthesis, common template have various carbohydrates, dispersing agent, surfactant with
And weak acid etc..For nanometer selenium made from template at agent stable homogeneous, granularity is small and scattered, but different templates method has part disadvantage
End, such as operates complicated, and time-consuming.
Therefore, using which kind of nanometer selenium and how in Rice Cropping, to solve nothing used in current Rice Cropping
Drawbacks described above existing for machine selenium and nanometer selenium, and the content of selenium and the content for reducing heavy metal in rice in rice are improved simultaneously,
It is current problem to be solved.
Summary of the invention
To solve the shortcomings and deficiencies of the prior art, the primary purpose of the present invention is that providing polysaccharide functionalized nano
Application of the selenium in selenium-rich rice plantation.
It another object of the present invention is to provide a kind of above-mentioned selenium-rich rice, is existed by above-mentioned polysaccharide functionalized nano selenium
Application in selenium-rich rice plantation obtains.
The object of the invention is achieved through the following technical solutions:
Application of the polysaccharide functionalized nano selenium in selenium-rich rice plantation, is that polysaccharide is used in selenium-rich rice planting process
Functionalized nano selenium.The invention is to find that polysaccharide functionalized nano selenium is answered in Rice Cropping based on inventor
With, can not only obtain selenium-rich rice, and can effective antagonism heavy metal absorption.
The polysaccharide functionalized nano selenium be preferably chitosan functionalized nano selenium, lentinan functionalized nano selenium and
One of pachymaran functionalized nano selenium or at least two；More preferably lentinan functionalized nano selenium.
Described includes making in different plantation periods using polysaccharide functionalized nano selenium in selenium-rich rice planting process
The position of rice is sprayed with polysaccharide functionalized nano selenium and/or be the soil for spraying rice cultivation.
The plantation period is preferably tillering stage and full heading time.
The position for spraying rice using polysaccharide functionalized nano selenium includes blade, stalk, root, pistil, spike of rice；
Preferably blade.
The dosage of the polysaccharide functionalized nano selenium is 0.1~10.0g/ mus, preferably 3g/ mus.
The polysaccharide functionalized nano selenium is that polysaccharide functionalized nano selenium is prepared into polysaccharide functionalization to receive when in use
Rice selenium aqueous solution is preferably prepared into the polysaccharide functionalized nano selenium aqueous solution that concentration is 0.05~0.15g/L, more preferably makes
The standby polysaccharide functionalized nano selenium aqueous solution for being 0.1g/L at concentration.
Specific step is as follows for the soil for spraying rice cultivation: before Rice Cropping, by polysaccharide functionalized nano
Selenium aqueous solution is sprayed in soil, rice cultivation and normal water and fertilizer management after polysaccharide functionalized nano selenium balances in the soil；
It is preferred that as follows: when carrying out whole soil before Rice Cropping, by polysaccharide functionalized nano selenium and base fertilizer in such a way that soil sprays
It is sprayed in soil together, rice cultivation and normal water and fertilizer management after polysaccharide functionalized nano selenium balances in the soil.
The balance takes preferably 1~2 week.
The specific plantation side at the described position for spraying rice using polysaccharide functionalized nano selenium in different plantation periods
Method are as follows: the mode of blade spraying is respectively adopted by polysaccharide function when paddy growth is to tillering stage and full heading time in rice cultivation
Nanometer selenium aqueous solution can be changed to be sprayed on rice leaf, and normal water and fertilizer management.
The step of mode of the blade spraying, is preferred are as follows: sprays the front in blade, reverse side or front and back sides, makes to spray
Blade face uniformly overwork solution, and blade does not drip.
A kind of selenium-rich rice is obtained by application of the above-mentioned polysaccharide functionalized nano selenium in selenium-rich rice plantation.
The content of cadmium is 0.1~0.510mg/kg in the selenium-rich rice brown rice.
The content of selenium is 0.082~0.520mg/kg in the selenium-rich rice brown rice.
The content of cadmium is less than 0.2mg/kg in the selenium-rich rice husk.
The content of selenium is 0.079~1.7mg/kg in the selenium-rich rice husk.
The content of cadmium is less than 0.2mg/kg in the selenium-rich rice stalk.
The content of selenium is 0.174~3.56mg/kg in the selenium-rich rice stalk.
To sum up, it is seen that application of the polysaccharide functionalized nano selenium provided through the invention in selenium-rich rice plantation obtains
Selenium-rich rice Se content is high, and whole body is available, and the husk and stalk of selenium-rich rice as mentioned can be further processed into feed,
Be conducive to livestock supplement selenium element.
Compared with prior art, the present invention has the following advantages and beneficial effects:
The present invention effectively improves the content of the selenium element of rice, simultaneously by polysaccharide functionalized nano selenium rice cultivation
Reduce the content of each heavy metal species in rice.
Detailed description of the invention
Fig. 1 is the transmission electron microscope picture of chitosan nano selenium；The size of right figure amplification is 2.5 times of left figure.
Fig. 2 is the transmission electron microscope picture of lentinan nanometer selenium；The size of right figure amplification is 2.5 times of left figure.
Fig. 3 is the transmission electron microscope picture of pachymaran nanometer selenium；The size of right figure amplification is 2.5 times of left figure.
Fig. 4 is polysaccharide nanometer selenium partial size Stable distritation figure in 20 days.
Fig. 5 is effect picture of the pachymaran functionalized nano selenium to Brown Rice antagonism Cd.
Fig. 6 is effect picture of the lentinan functionalized nano selenium to Brown Rice antagonism Cd.
Fig. 7 is effect picture of the chitosan functionalized nano selenium to Brown Rice antagonism Cd.
Fig. 8 is the testing result figure of Se content in the Brown Rice obtained using pachymaran functionalized nano selenium.
Fig. 9 is the testing result figure of Se content in the Brown Rice obtained using lentinan functionalized nano selenium.
Figure 10 is the testing result figure of Se content in the Brown Rice obtained using chitosan functionalized nano selenium.
Figure 11 is effect picture of the pachymaran functionalized nano selenium to the husk of paddy rice antagonism Cd.
Figure 12 is effect picture of the lentinan functionalized nano selenium to the husk of paddy rice antagonism Cd.
Figure 13 is effect picture of the chitosan functionalized nano selenium to the husk of paddy rice antagonism Cd.
Figure 14 is the testing result figure of Se content in the rice rice husk obtained using pachymaran functionalized nano selenium.
Figure 15 is the testing result figure of Se content in the rice rice husk obtained using lentinan functionalized nano selenium.
Figure 16 is the testing result figure of Se content in the rice rice husk obtained using chitosan functionalized nano selenium.
Figure 17 is effect picture of the pachymaran functionalized nano selenium to rice straw antagonism Cd.
Figure 18 is effect picture of the lentinan functionalized nano selenium to rice straw antagonism Cd.
Figure 19 is effect picture of the chitosan functionalized nano selenium to rice straw antagonism Cd.
Figure 20 is the testing result figure of Se content in the rice straw obtained using pachymaran functionalized nano selenium.
Figure 21 is the testing result figure of Se content in the rice straw obtained using lentinan functionalized nano selenium.
Figure 22 is the testing result figure of Se content in the rice straw obtained using chitosan functionalized nano selenium.
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
Experimental cultivation rice of the present invention is day excellent 390, is bought in Guangdong gold seed rice industry Co., Ltd, in March, 2017 kind
Plant early rice.
Preparation method reference literature Tianqi Nie, the Hualian Wu of polysaccharide functionalized nano selenium used in the present invention,
KaHing Wong,et al.Facile synthesis of highly uniform selenium nanoparticles
using glucose as the reductant and surface decorator to induce cancer cell
Apoptosis [J] .Journal of Materials Chemistry B, 2016,4 (13): 2351-2358, polysaccharide therein
Respectively chitosan (Mn=30000), lentinan (Mn=1150) and pachymaran (Mn=1000), obtained polysaccharide are received
Rice selenium is detected by transmission electron microscope, and as shown in figure 1 to 3, wherein Fig. 1 is the transmission electron microscope picture of chitosan nano selenium to testing result,
Fig. 2 is the transmission electron microscope picture of lentinan nanometer selenium, and Fig. 3 is the transmission electron microscope picture of pachymaran nanometer selenium, by result as it can be seen that three
Kind nanometer selenium all has good dispersibility.
The 1mg polysaccharide nanometer selenium being prepared is dissolved in the water of 2mL, it is more then to measure mushroom by Malvern ParticleSizer
Sugared nanometer selenium, chitosan nano selenium, pachymaran nanometer selenium its grain size stability situation in 20 days in water, as a result such as Fig. 4 institute
Show, it is seen that the partial size maximum of chitosan nano selenium reaches 164nm, and the partial size of lentinan nanometer selenium is 114nm, pachymaran
The partial size of nanometer selenium minimum 86nm, polysaccharide nanometer selenium is all with good stability in three.
Embodiment
One, the rice cultivation in such a way that rice leaf sprays:
Rice cultivation 100m2, after paddy growth to tillering stage (35d or so after transplanting seedlings) and full heading time (after transplanting seedlings
70d or so) when, the mode that blade spraying is respectively adopted carries out spraying concentration in the front and back sides of rice leaf or front or back and is
0.1g/L polysaccharide functionalized nano selenium aqueous solution (including chitosan functionalized nano selenium, lentinan functionalized nano selenium or
Pachymaran functionalized nano selenium), blade face uniformly overworks solution when spraying, and rice leaf does not drip, each blade spraying
When polysaccharide functionalized nano selenium the amount of spraying be 0.0g/ mus, 0.5g/ mus, 1.5g/ mus, 3.0g/ mus, then normal water and fertilizer management.
The blade position that specifically sprays, the type of the polysaccharide functionalized nano selenium sprayed, the amount of spraying of polysaccharide functionalized nano selenium are shown in Table 1
In embodiment 1-6,11-16,21-26.
Two, mode rice cultivation is sprayed using soil:
In site preparation, in such a way that soil sprays by polysaccharide functionalized nano selenium (including chitosan functionalized nano selenium,
Lentinan functionalized nano selenium or pachymaran functionalized nano selenium) it is applied in soil together with base fertilizer, wherein spraying use
The concentration of polysaccharide functionalized nano selenium aqueous solution be 0.1g/L, the amount of spraying of polysaccharide functionalized nano selenium is 0.0g/ mus,
3.0g/ mus, 5.0g/ mus, 10.0g/ mus, the rice cultivation after polysaccharide functionalized nano selenium and base fertilizer balance 2 weeks in the soil
100m2, then normal water and fertilizer management.The spray of the type, polysaccharide functionalized nano selenium of the polysaccharide functionalized nano selenium specifically sprayed
The amount of applying is shown in Table the embodiment 7~9,17~19,27~29 in 1.
Three, simultaneously in such a way that soil sprays and rice leaf sprays rice cultivation:
In site preparation, in such a way that soil sprays by polysaccharide functionalized nano selenium (including chitosan functionalized nano selenium,
Lentinan functionalized nano selenium or pachymaran functionalized nano selenium) it is applied in soil together with base fertilizer, wherein spraying use
The concentration of polysaccharide functionalized nano selenium aqueous solution be 0.1g/L, the amount of spraying of polysaccharide functionalized nano selenium is 5.0g/ mus, to more
Rice cultivation 100m after sugared functionalized nano selenium and base fertilizer balance 2 weeks in the soil2, after paddy growth to tillering stage (after transplanting seedlings
35d or so) and full heading time (70d or so after transplanting seedlings), the mode of blade spraying is respectively adopted in the positive and negative of rice leaf
Face carries out the polysaccharide functionalized nano selenium aqueous solution that spraying concentration is 0.1g/L, and (including chitosan functionalized nano selenium, mushroom are more
Sugared functionalized nano selenium or pachymaran functionalized nano selenium), the tow sides of blade all uniformly overwork solution, and water when spraying
Rice blade does not drip, and the amount of spraying of polysaccharide functionalized nano selenium is 1.5g/ mus when each blade spraying, then normal liquid manure pipe
Reason.The type of the polysaccharide functionalized nano selenium specifically sprayed is shown in Table the embodiment 10,20,30 in 1.
1 polysaccharide functionalized nano selenium antagonism heavy metal Rice Cropping embodiment of table
Note: additive amount is calculated in polysaccharide functionalized nano selenium with the content of Se.
Four, testing result
(1) heavy metal content in soil analysis and implementation field conditions
Test point is carried out using ICP (Atomic Emission Spectrometer AES) to Cd, Cr, Ni, Pb, Cu, Zn, Se content in initial soil
Analysis, the results are shown in Table 2.The average content of Cd is 0.313mgkg in the test plot-1, referring to soil environment quality secondary standard
(GB 15618-1995), the exceeded pedotheque number of Cd is up to 63.33% in topsoil, exceeded between 1-2 times, belongs to
In slight degree pollution；And the content of Cr, Ni, Pb, Cu, Zn is not exceeded in soil；The average content of Se is in soil
0.466mg·kg-1, according to national standard, the content of Se is greater than 0.4mgkg in soil-1When as selenium-rich soil, so this
Block experimental plot belongs to selenium-rich soil, can be widely applied to the growth of Se-rich farm products.
Cd, Cr, Ni, Pb, Cu, Zn content in 2 soil of table
Note: being national soil quality standard secondary standard Limited Doses in bracket.
(2) in Brown Rice heavy metal and selenium content
After polysaccharide functionalized nano selenium antagonism heavy metal Rice Cropping rice harvesting, it is utilized respectively ICP atomic emission spectrum
Instrument carries out the analysis test of heavy metal Cd in brown rice, Pb, Zn, Cu, Ni, Cr, Se content.
Specific visible Fig. 5~7 of the polysaccharide functionalized nano selenium effect picture short of money to rice antagonism Cd.Test result shows, with
Blank control is compared, with polysaccharide functionalized nano selenium addition and add the increase of concentration, the content of Cd is successively in brown rice
Decline, thus it is speculated that after polysaccharide functionalized nano selenium enters families of plant, in conjunction with free Cd2+Selenium-albumen-cadmium compound is formed, is reduced
Cd2+Transfer to brown rice.Due to selenium cadmium compound formation and reduce free Cd2+Concentration, to reduce the poison of cadmium
Property, selenium is shown as to the antagonism of cadmium toxicity.The biological effectiveness of blade spraying polysaccharide functionalized nano selenium is substantially better than soil
The effect of earth addition, blade spraying polysaccharide functionalized nano selenium can be directly entered in plant by the stomata of rice leaf,
Selenium has apparent antagonism to the absorption of cadmium, to reduce the accumulation of Cd in brown rice well, wherein embodiment 23,
24,25 and 19 safety compliances for realizing brown rice Cd content produce.
Three kinds of polysaccharide functionalized nano selenium materials are optimal with the effect of pachymaran functionalized nano selenium antagonism heavy metal Cd,
Followed by lentinan functionalized nano selenium.
3.0g (Se)/mu progress leave dual sides spray pachymaran functionalized nano selenium according to quantity, 1.5g (Se)/mu blade
Front is sprayed sprayed with 1.5g (Se)/mu blade reverse side after, in excellent 390 brown rice in the rice day of plantation Cd content and blank control according to
It is secondary to reduce 55.70%, 70.30%, 64.08%, in brown rice the content of Cd be followed successively by 0.142mg/kg, 0.171mg/kg,
0.158mg/kg, the brown rice Cd content of output have been lower than national food safety standard (GB2762-2012) value 0.2mg/kg；It presses
After 10g (Se)/mu addition, Cd content reduces 56.81% compared with blank control in excellent 390 brown rice in the rice day of plantation, rough
The content of Cd drops to 0.206mg/kg in rice.
3g (Se)/mu is carried out after leave dual sides spray lentinan functionalized nano selenium according to quantity, and the rice day of plantation is excellent
Cd content and blank control reduce 55.97% in 390 brown rice, and the content of Cd is dropped to by 0.477mg/kg in brown rice
0.210mg/kg；After 10g (Se)/mu addition, Cd content reduces compared with blank control in excellent 390 brown rice in the rice day of plantation
71.7%, the content of Cd drops to 0.135mg/kg in brown rice, and the brown rice Cd content of output has been lower than food safety country mark
Quasi- (GB2762-2012) value 0.2mg/kg.
Selenium-rich paddy national standard (DB/T 22499-2008) regulation, it is big that sample is processed into three-level as defined in GB 1354
Rice is executed by GB/T 5009.93.The rice checking result Se content of selenium-rich paddy processing is between 0.04~0.30mg/kg
, it is determined as selenium-rich paddy；Inspection result Se content is determined as non-selenium-rich paddy less than 0.04mg/kg；Inspection result selenium contains
Amount is determined as the exceeded paddy of selenium content, should not eat greater than 0.3mg/kg.
Polysaccharide functionalized nano selenium acts on the content of Se in lower output brown rice specifically visible Fig. 8~10.Since the test is shown
Model field belongs to selenium-rich soil, can produce selenium-enriched crop, and the content of brown rice Se is 0.168mg/kg in blank control, is that selenium-rich is big
Rice.According to analysis of experimental data, blade spraying polysaccharide functionalized nano selenium considerably increases the content of Se in brown rice, improves rough
The quality of rice；And after soil addition polysaccharide functionalized nano selenium, reduce the content of Se in Brown Rice.This is mainly by selenium cadmium
Caused by antagonism, currently it is intracorporal to show that soil spraying selenium can reduce plant for the result of study about selenium cadmium antagonism
Cadmium content mainly has the reason of following three aspect, and the formation of selenium cadmium indissoluble object reduces the validity of cadmium in soil in the soil；
In plant root selenium cadmium macromolecular indissoluble object, since this indissoluble object cannot be by casparian strip, to reduce cadmium to overground part
Transport；Reinforce complexing of the cysteine to cadmium ion in plant, reduces its transhipment into seed.
Under the action of pachymaran functionalized nano selenium, in Brown Rice the luffing of Se content be 0.082~
0.257mg/kg；Under the action of lentinan functionalized nano selenium, in Brown Rice the luffing of Se content be 0.093~
0.510mg/kg；Under the action of chitosan functionalized nano selenium, in Brown Rice the luffing of Se content be 0.111~
0.307mg/kg.In addition to lentinan functionalized nano selenium carries out the water that leave dual sides spray output with 3g/ mus of additive amount
Rice brown rice Se content is exceeded outer, under the action of external source selenium, the Brown Rice of output between 0.04~0.30mg/kg,
It is determined as selenium-enriched rice.The toxic action for reducing heavy metal cadmium soil to rice is realized, and produces and obtains high value
Selenium-enriched crop.
Under the action of polysaccharide functionalized nano selenium, the content of Pb, Zn, Cu, Ni, Cr is not exceeded in Brown Rice, with
Blank control does not have significant difference.
(3) in the husk of paddy rice heavy metal and selenium content
After the harvesting of polysaccharide functionalized nano selenium antagonism heavy metal Rice Cropping Study On Rice, pass through ICP atomic emission spectrum
Instrument carries out the analysis test of heavy metal Cd in husk, Pb, Zn, Cu, Ni, Cr, Se content respectively.
Figure 11~13 are effect picture of the polysaccharide functionalized nano selenium to the husk of paddy rice antagonism Cd.Compared with blank control, with
The addition of polysaccharide functionalized nano selenium and add the increase of concentration, the content of Cd successively declines in husk, and blade spraying is more
The biological effectiveness of sugared functionalized nano selenium is substantially better than the effect of soil addition, and the content of Cd is respectively less than in all husks
0.2mg/kg is lower than forage standard (GB 13078-2001), and the content of Pb, Zn, Cu, Ni, Cr is not also exceeded in husk,
There is no significant difference with blank control.
3g (Se)/mu leave dual sides spray pachymaran functionalized nano selenium according to quantity, 1.5g (Se)/mu face of blade spray
It applies, after 1.5g (Se)/mu blade reverse side sprays, Cd content is successively reduced with blank control in excellent 390 husk in the rice day of plantation
69.54%, 66.24%, 66.72%；After 10g (Se)/mu addition, in excellent 390 husk in the rice day of plantation Cd content with
Blank control, which is compared, reduces 58.66%.
3g (Se)/mu is carried out after leave dual sides spray lentinan functionalized nano selenium according to quantity, and the rice day of plantation is excellent
Cd content and blank control reduce 50.51% in 390 husks, and the content of Cd is dropped to by 0.16mg/kg in husk
0.079mg/kg；After 10g (Se)/mu addition, Cd content reduces compared with blank control in excellent 390 husk in the rice day of plantation
64.77%, the content of Cd drops to 0.056mg/kg in husk.
3g (Se)/mu leave dual sides spray chitosan functionalized nano selenium according to quantity, 1.5g (Se)/mu face of blade spray
It applies, after 1.5g (Se)/mu blade reverse side sprays, Cd content is successively reduced with blank control in excellent 390 husk in the rice day of plantation
30.17%, 41.15%, 38.90%.
Polysaccharide functionalized nano selenium acts on the content of Se in lower output the husk of paddy rice specifically visible Figure 14~16.It is more in Poria cocos
Under the action of sugared functionalized nano selenium, it is blank control water that the luffing of Se content, which is 0.202~0.611mg/kg, in the husk of paddy rice
1.38~4.18 times of Se content in rice hulls；Under the action of lentinan functionalized nano selenium, Se content in Brown Rice
Luffing be 0.253~1.70mg/kg, be 1.73~11.7 times of Se content in blank control the husk of paddy rice；In chitosan function
Under the action of nanometer selenium capable of being changed, it is blank control rice paddy that the luffing of Se content, which is 0.154~1.65mg/kg, in Brown Rice
1.05~11.3 times of Se content in shell.The content of heavy metal is below forage standard (GB 13078-2001) in the husk of paddy rice,
The husk of these high selenium can be used as animal feed.Animal immunizing power can be improved in organic selenium feed, enhances disease resistance, selenium energy
The toxic action of antagonism heavy metallic poison element cadmium, mercury, lead, arsenic, has detoxication to aflatoxin, makes its degradation, loses
Carcinogenesis and exclude rapidly external, can also have detoxication to certain bacteriotoxins, such as fusarium toxin.
(4) in rice straw heavy metal and selenium content (in terms of butt)
After the harvesting of polysaccharide functionalized nano selenium antagonism heavy metal Rice Cropping Study On Rice, pass through ICP atomic emission spectrum
Instrument carries out the analysis test of heavy metal Cd in stalk, Pb, Zn, Cu, Ni, Cr, Se content respectively.
Figure 17~19 are effect picture of the polysaccharide functionalized nano selenium to rice straw antagonism Cd.Chitosan functionalized nano selenium
To the effect and blank control of rice straw antagonism heavy metal Cd, significant difference is not present；And pachymaran functionalized nano
Under selenium and the effect of lentinan functionalized nano selenium, the rice straw of output carries out leave dual sides spray compared with blank control
Applying polysaccharide functionalized nano selenium reduces the content of Cd in rice straw, because the blade addition of Se inhibits Cd from rice root
Transfer to stem；Under conditions of the amount of soil addition polysaccharide functionalized nano selenium is 3g/ mus or 5g/ mus, rice straw
The content of middle Cd dramatically increases, because promoting PCs isochela hop protein after a small amount of Se in soil enters plant
Synthesis, to promote transfer of the Cd to stem；Under conditions of the amount of soil addition polysaccharide functionalized nano selenium is 10g/ mus,
In rice straw the content of Cd is to significantly reduce, because under the action of sufficient amount Exogenous selenium, selenium cadmium indissoluble object in the soil
Formation reduces in soil the validity of cadmium and in plant root selenium cadmium macromolecular indissoluble object, since this indissoluble object cannot lead to
Casparian strip is crossed, to reduce transport of the cadmium to overground part.
And the content of Pb, Zn, Cu, Ni, Cr is not also exceeded in husk, does not have significant difference with blank control.
3g (Se)/mu leave dual sides spray pachymaran functionalized nano selenium according to quantity, 1.5g (Se)/mu leave dual sides
It spraying, 1.5g (Se)/mu face of blade sprays, after 1.5g (Se)/mu blade reverse side sprays, excellent 390 stalk in the rice day of plantation
Middle Cd content and blank control in turn reduce 55.26%, 44.80%, 54.93%, 54.35%；By 10g (Se)/mu addition
Afterwards, Cd content reduces 37.50% compared with blank control in excellent 390 stalk in the rice day of plantation.
3g (Se)/mu is carried out after leave dual sides spray lentinan functionalized nano selenium according to quantity, and the rice day of plantation is excellent
Cd content and blank control reduce 49.01% in 390 stalks, and the content of Cd is dropped to by 0.16mg/kg in husk
0.079mg/kg；After 10g (Se)/mu addition, Cd content reduces compared with blank control in excellent 390 stalk in the rice day of plantation
65.34%.
Figure 20~22 are the effect pictures that polysaccharide functionalized nano selenium acts on lower output rice straw accumulation Se.In blank control
The content of stalk Se (in terms of butt) is 0.159mg/kg, under the action of pachymaran functionalized nano selenium, rice straw
The luffing of middle Se content is 0.145~0.892mg/kg；Under the action of lentinan functionalized nano selenium, Se in rice straw
The luffing of content is 0.136~3.56mg/kg；Under the action of chitosan functionalized nano selenium, Se content in Brown Rice
Luffing is 0.174~1.702mg/kg.Under the action of blade applies polysaccharide functionalized nano selenium, rice straw are greatly increased
Largely there is Se in rice and dissociate in conjunction with Cd Cd in the content of Se in stalk2+Selenium-albumen-cadmium compound is formed, Cd is reduced2+To
The transfer of husk and brown rice.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. application of the polysaccharide functionalized nano selenium in selenium-rich rice plantation, which is characterized in that in selenium-rich rice planting process
Use polysaccharide functionalized nano selenium.
2. application of the polysaccharide functionalized nano selenium according to claim 1 in selenium-rich rice plantation, which is characterized in that institute
The polysaccharide functionalized nano selenium stated is chitosan functionalized nano selenium, lentinan functionalized nano selenium and pachymaran functionalization
One of nanometer selenium or at least two.
3. application of the polysaccharide functionalized nano selenium according to claim 2 in selenium-rich rice plantation, which is characterized in that institute
The dosage for the polysaccharide functionalized nano selenium stated is 0.1~10.0g/ mus.
4. application of the polysaccharide functionalized nano selenium according to claim 3 in selenium-rich rice plantation, which is characterized in that institute
The polysaccharide functionalized nano selenium stated is that polysaccharide functionalized nano selenium is prepared into concentration for the more of 0.05~0.15g/L when in use
Sugared functionalized nano selenium aqueous solution.
5. application of the polysaccharide functionalized nano selenium according to any one of claims 1 to 4 in selenium-rich rice plantation, special
Sign is that described includes using in different plantation periods using polysaccharide functionalized nano selenium in selenium-rich rice planting process
Polysaccharide functionalized nano selenium sprays the position of rice and/or is the soil for spraying rice cultivation.
6. application of the polysaccharide functionalized nano selenium according to claim 5 in selenium-rich rice plantation, which is characterized in that
It is described using polysaccharide functionalized nano selenium to spray the soil of rice cultivation specific step is as follows: before Rice Cropping,
Polysaccharide functionalized nano selenium aqueous solution is sprayed in soil, the rice cultivation after polysaccharide functionalized nano selenium balances in the soil
And normal water and fertilizer management.
7. application of the polysaccharide functionalized nano selenium according to claim 6 in selenium-rich rice plantation, which is characterized in that
The specific implantation methods of the soil that rice cultivation is sprayed using polysaccharide functionalized nano selenium are as follows: before Rice Cropping
When carrying out whole soil, polysaccharide functionalized nano selenium is sprayed in soil together with base fertilizer in such a way that soil sprays, to polysaccharide
Rice cultivation and normal water and fertilizer management after functionalized nano selenium balances in the soil.
8. application of the polysaccharide functionalized nano selenium according to claim 5 in selenium-rich rice plantation, which is characterized in that institute
The plantation period stated is tillering stage and full heading time；The position for spraying rice using polysaccharide functionalized nano selenium includes
Blade, stalk, root, pistil, spike of rice.
9. application of the polysaccharide functionalized nano selenium according to claim 8 in selenium-rich rice plantation, which is characterized in that
The specific implantation methods that rice leaf rice cultivation is sprayed using polysaccharide functionalized nano selenium are as follows: rice cultivation,
When paddy growth is to tillering stage and full heading time, polysaccharide functionalized nano selenium aqueous solution is sprayed by the way of blade spraying
It imposes on rice leaf, and normal water and fertilizer management；
The step of mode of the blade spraying are as follows: spray the front in blade, reverse side or front and back sides, make the blade face sprayed
Solution uniformly is overworked, and blade does not drip.
10. a kind of selenium-rich rice, which is characterized in that be by polysaccharide functionalized nano selenium according to any one of claims 1 to 9
Application in selenium-rich rice plantation obtains.
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