CN109580511A - A kind of detection method and detection kit of high-density lipoprotein cholesterol - Google Patents
A kind of detection method and detection kit of high-density lipoprotein cholesterol Download PDFInfo
- Publication number
- CN109580511A CN109580511A CN201811487662.0A CN201811487662A CN109580511A CN 109580511 A CN109580511 A CN 109580511A CN 201811487662 A CN201811487662 A CN 201811487662A CN 109580511 A CN109580511 A CN 109580511A
- Authority
- CN
- China
- Prior art keywords
- density lipoprotein
- enzymatic reagent
- lipoprotein cholesterol
- detection method
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The present invention is suitable for technical field of biological, provide the detection method and detection kit of a kind of high-density lipoprotein cholesterol, detection method uses rate determination method, 500~546nm of dominant wavelength is set, at 500~546nm of dominant wavelength, the increase rate of absorbance is directly proportional to the activity of sample middle-high density lipoprotein cholesterol in reaction system, postpones 60~90s, then the absorbance increase rate for measuring 60~150s, obtains the concentration of high-density lipoprotein cholesterol.Detection kit includes enzymatic reagent 1 and enzymatic reagent 2, and the volume ratio of enzymatic reagent 1 and enzymatic reagent 2 is 2.8~3.3:1.Whereby, the present invention can exclude the interference of serum background, improve accuracy in detection, while shortening the reaction time, improve reaction efficiency.
Description
Technical field
The present invention relates to technical field of biological, and in particular to a kind of detection method of high-density lipoprotein cholesterol and
Detection kit.
Background technique
High-density lipoprotein cholesterol (hereinafter referred to as HDL-C) refers to the cholesterol that high-density lipoprotein molecule is taken, and is
The endogenous cholesterol ester of antiport, is transported into liver, then clear out of blood.Therefore, HDL-C can anti-atherogenic it is hard
Change, the danger for suffering from coronary heart disease can be reduced.
In the prior art, the measuring method of HDL-C mainly includes immune partition method, polyanion masking method and enzyme modification
Method etc., these methods are all made of end-point method measurement, and end-point method measurement is interfered vulnerable to serum background, and the reaction time is long, at least
5min is needed, the accuracy and determination efficiency of result are leveraged.
In summary, the prior art there will naturally be shortcoming in practical applications, it is necessary to be improved.
Summary of the invention
In view of the above technical defects, the purpose of the present invention is to provide a kind of detection methods of high-density lipoprotein cholesterol
And detection kit, rate determination method is used, the interference of serum background can be excluded, accuracy in detection is improved, shortens simultaneously
Reaction time improves reaction efficiency.
To achieve the goals above, the present invention provides a kind of detection method of high-density lipoprotein cholesterol, the detection
Method uses rate determination method, and 500~546nm of dominant wavelength, at 500~546nm of dominant wavelength, absorbance in reaction system is arranged
The activity of increase rate and sample middle-high density lipoprotein cholesterol it is directly proportional, postpone 60~90s, then measure 60~
The absorbance increase rate of 150s, obtains the concentration of high-density lipoprotein cholesterol；
Detecting step is as follows:
A takes blank tube, standard pipe and sample tube, enzymatic reagent 1 is all separately added into three kinds of pipes, then in blank tube, standard
Corresponding distilled water, titer and the sample that same volume is added, is incubated for 3~5min in pipe and sample tube after mixing；Again to three kinds
It is all separately added into enzymatic reagent 2 in pipe, mixes, obtains reaction solution；
After reaction solution described in B postpones 60~90s, absorbance is read at 500~546nm of dominant wavelength, is read again after reacting 60s
Take absorbance, calculate mean light absorbency change rate, obtain blank tube, in standard pipe and sample tube reaction solution absorbance per minute
Change rate；
The concentration of C calculating high-density lipoprotein cholesterol.
A kind of detection method of high-density lipoprotein cholesterol according to the present invention, the enzymatic reagent 1 include cholesterol oxygen
Change enzyme and peroxidase, the enzyme activity of the cholesterol oxidase is 1.4~1.8KU/L, the enzyme activity of the peroxidase
Power is 1.8~2.2KU/L.
A kind of detection method of high-density lipoprotein cholesterol according to the present invention, the enzymatic reagent 2 include cholesterol ester
Enzyme, the enzyme activity of the cholesteryl esterase are 1.8~2.2KU/L.
A kind of detection method of high-density lipoprotein cholesterol according to the present invention, the enzymatic reagent 1 and the enzymatic reagent 2
Volume ratio be 2.8~3.3:1.
A kind of detection method of high-density lipoprotein cholesterol according to the present invention, the enzymatic reagent 1 and enzymatic reagent 2 it is total
Volume and the volume ratio of the sample are 100~120:1.
A kind of detection method of high-density lipoprotein cholesterol according to the present invention, the detection method also set up commplementary wave length
700~800nm.
A kind of detection method of high-density lipoprotein cholesterol according to the present invention, the concentration of the titer is 2~
4mmol/L。
A kind of detection method of high-density lipoprotein cholesterol according to the present invention, the present invention also provides one kind for described
The detection kit of detection method.
A kind of detection kit according to the present invention, the detection kit include enzymatic reagent 1 and enzymatic reagent 2, the enzyme
The volume ratio of reagent 1 and the enzymatic reagent 2 is 2.8~3.3:1.
A kind of detection kit according to the present invention, the enzymatic reagent 1 include cholesterol oxidase and peroxidase, institute
The enzyme activity for stating cholesterol oxidase is 1.4~1.8KU/L, and the enzyme activity of the peroxidase is 1.8~2.2KU/L.
A kind of detection kit according to the present invention, the enzymatic reagent 2 include cholesteryl esterase, the cholesteryl esterase
Enzyme activity is 1.8~2.2KU/L.
The invention has the benefit that
1, the present invention uses rate determination method, and measurement is the rate reacted, and the linear phase during the reaction is read
Number is different from end assay method, can exclude the interference of sample background, improve the accuracy of result.
2, the present invention uses rate determination method, and delay time is short, and the reading duration is also very short, makes the entire reaction time by original
5~10min shorten to 2~3min, improve test efficiency.
3, operation of the present invention is easy, and instrument parameter setting is simple, reduces the detection technique of high-density lipoprotein cholesterol
Difficulty.
4, the present invention also sets up commplementary wave length in addition to dominant wavelength, and high-density lipoprotein gallbladder is measured at 700~800nm of commplementary wave length
The concentration of sterol；It is detected using dual wavelength, the physical disturbances such as the noise of sample background can be eliminated, can also eliminate it
His specificity is interfered and nonspecific interference.
5, detection kit of the invention can be used in automatic clinical chemistry analyzer, semi-automatic biochemical analyzer etc., be applicable in
Range is wide；It can directly use on the machine, be no longer needed to by other operations such as dilution simultaneously.
Detailed description of the invention
Fig. 1 is the response curve of prior art measurement；
Fig. 2 is the response curve that the embodiment of the present invention 1 measures；
Fig. 3 is the response curve that the embodiment of the present invention 2 measures；
Fig. 4 is the response curve that the embodiment of the present invention 3 measures.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, not
For limiting the present invention.
The present invention provides a kind of detection methods of high-density lipoprotein cholesterol to be arranged main wave using rate determination method
Long 500~546nm, at 500~546nm of dominant wavelength, the increase rate of absorbance and sample middle-high density in reaction system
The activity of lipoprotein cholesterol is directly proportional, postpones 60~90s, then measures the absorbance increase rate of 60~150s, obtains sample
The concentration of this middle-high density lipoprotein cholesterol.
Using rate determination method measure be reaction rate, the linear phase during the reaction read, Ke Yipai
Except the interference of sample background, the accuracy of result is improved；Delay time is short, and the reading duration is also very short, thus makes entire anti-
It is greatly shortened between seasonable, 2~3min is shorten to by 5 original~10min, improves test efficiency；It is easy to operate, instrument parameter
Setting is simple, reduces the detection technique difficulty of high-density lipoprotein cholesterol；It can be suitable for various full-automatic, semiautomatic biochemistries
Analyzer and spectrophotometer, it is applied widely.
Detecting step is as follows:
A takes blank tube, standard pipe and sample tube, enzymatic reagent 1 is all separately added into three kinds of pipes, then in blank tube, standard
Corresponding distilled water, titer and the sample that same volume is added, mixes in pipe and sample tube, and 3~5min is incubated under the conditions of 37 DEG C；
Enzymatic reagent 2 is all separately added into three kinds of pipes again, mixes, obtains reaction solution.
After B reaction solution postpones 60~90s under the conditions of 37 DEG C, absorbance A 1 is read at 500~546nm of dominant wavelength, then
Absorbance A 2 is read after reacting 60s, mean light absorbency change rate Δ A/min is calculated, obtains in blank tube, standard pipe and sample tube
The absorbance change rate per minute of reaction solution.
The concentration of C calculating high-density lipoprotein cholesterol
Enzymatic reagent 1 includes cholesterol oxidase and peroxidase, and the enzyme activity of cholesterol oxidase is 1.4~1.8KU/
L, the enzyme activity of peroxidase is 1.8~2.2KU/L, the volume ratio of cholesterol oxidase and peroxidase is 0.8~
1.2:1；Enzymatic reagent 2 includes cholesteryl esterase, and the enzyme activity of cholesteryl esterase is 1.8~2.2KU/L；Enzymatic reagent 1 and enzymatic reagent 2
Volume ratio be 2.8~3.3:1.
Titer is high-density lipoprotein cholesterol solution, and concentration is 2~4mmol/L.
Sample is fresh not haemolysis serum or blood plasma；Sample can stablize storage 6~8 days, -20 DEG C under the conditions of 2~8 DEG C
Under the conditions of can stablize storage 30~35 days.
The volume ratio of the total volume and sample of enzymatic reagent 1 and enzymatic reagent 2 is 100~120:1.
The Direction of Reaction is positive reaction.
Concentration of the present invention in addition to measuring high-density lipoprotein cholesterol at 500~546nm of dominant wavelength, also in commplementary wave length
The concentration of high-density lipoprotein cholesterol is measured at 700~800nm；It is detected using dual wavelength, mainly reads dominant wavelength-pair
The absorbance change rate of the response line of wavelength, can eliminate the physical disturbances such as the noise of sample background, can also eliminate other
Specificity interference and nonspecific interference.
The present invention also provides a kind of detection kits for above-mentioned detection method, including enzymatic reagent 1 and enzymatic reagent 2；
Enzymatic reagent 1 includes cholesterol oxidase and peroxidase, and the enzyme activity of cholesterol oxidase is 1.4~1.8KU/L, peroxidating
The enzyme activity of object enzyme is 1.8~2.2KU/L, and the volume ratio of cholesterol oxidase and peroxidase is 0.8~1.2:1；Enzyme examination
Agent 2 includes cholesteryl esterase, and the enzyme activity of cholesteryl esterase is 1.8~2.2KU/L；Enzymatic reagent 1 and the volume ratio of enzymatic reagent 2 are
2.8~3.3:1.
Application method: it can be used for automatic clinical chemistry analyzer, semi-automatic biochemical analyzer etc.；It can directly use on the machine, nothing
It need to be using other operations such as dilution.
Condition of storage and validity period are as follows: preservation condition is 2~8 DEG C, and validity period is 12 months.
To obtain preferred forms, following several embodiments are arranged in the present invention；Following embodiment is only listed and above-mentioned step
Different parts, identical content are no longer listed in rapid.
Embodiment 1
A takes blank tube, standard pipe and sample tube, enzymatic reagent 1 is all separately added into three kinds of pipes, then in blank tube, standard
Corresponding distilled water, titer and the sample that same volume is added, is incubated for 3~5min in pipe and sample tube after mixing；Again to three kinds
It is all separately added into enzymatic reagent 2 in pipe, mixes, obtains reaction solution；
After reaction solution described in B postpones 60~90s, absorbance is read at 500~546nm of dominant wavelength, is read again after reacting 60s
Take absorbance, calculate mean light absorbency change rate, obtain blank tube, in standard pipe and sample tube reaction solution absorbance per minute
Change rate；
The concentration of C calculating high-density lipoprotein cholesterol.
The enzyme activity of cholesterol oxidase is 1.58KU/L, and the enzyme activity of peroxidase is 1.84KU/L, cholesterol ester
The enzyme activity of enzyme is 1.92KU/L, and the volume ratio of enzymatic reagent 1 and enzymatic reagent 2 is 3.12:1, the totality of enzymatic reagent 1 and enzymatic reagent 2
The long-pending volume ratio with sample is 109:1.
The response curve in the above way measuring high-density lipoprotein cholesterol is shown in Fig. 2.
Embodiment 2
A takes blank tube, standard pipe and sample tube, enzymatic reagent 1 is all separately added into three kinds of pipes, then in blank tube, standard
Corresponding distilled water, titer and the sample that same volume is added, is incubated for 3~5min in pipe and sample tube after mixing；Again to three kinds
It is all separately added into enzymatic reagent 2 in pipe, mixes, obtains reaction solution；
After reaction solution described in B postpones 60~90s, absorbance is read at 500~546nm of dominant wavelength, is read again after reacting 60s
Take absorbance, calculate mean light absorbency change rate, obtain blank tube, in standard pipe and sample tube reaction solution absorbance per minute
Change rate；
The concentration of C calculating high-density lipoprotein cholesterol.
The enzyme activity of cholesterol oxidase is 1.6KU/L, and the enzyme activity of peroxidase is 1.95KU/L, cholesteryl esterase
Enzyme activity be 1.87KU/L, the volume ratio of enzymatic reagent 1 and enzymatic reagent 2 is 2.89:1, the total volume of enzymatic reagent 1 and enzymatic reagent 2
Volume ratio with sample is 113:1.
The response curve in the above way measuring high-density lipoprotein cholesterol is shown in Fig. 3.
Embodiment 3
A takes blank tube, standard pipe and sample tube, enzymatic reagent 1 is all separately added into three kinds of pipes, then in blank tube, standard
Corresponding distilled water, titer and the sample that same volume is added, is incubated for 3~5min in pipe and sample tube after mixing；Again to three kinds
It is all separately added into enzymatic reagent 2 in pipe, mixes, obtains reaction solution；
After reaction solution described in B postpones 60~90s, absorbance is read at 500~546nm of dominant wavelength, is read again after reacting 60s
Take absorbance, calculate mean light absorbency change rate, obtain blank tube, in standard pipe and sample tube reaction solution absorbance per minute
Change rate；
The concentration of C calculating high-density lipoprotein cholesterol.
The enzyme activity of cholesterol oxidase is 1.66KU/L, and the enzyme activity of peroxidase is 2.03KU/L, cholesterol ester
The enzyme activity of enzyme is 2.12KU/L, and the volume ratio of enzymatic reagent 1 and enzymatic reagent 2 is 3.04:1, the totality of enzymatic reagent 1 and enzymatic reagent 2
The long-pending volume ratio with sample is 116:1.
The response curve in the above way measuring high-density lipoprotein cholesterol is shown in Fig. 4.
The implementation procedure of other embodiments repeats no more, and obtained response curve is also no longer listed one by one, each embodiment its
The data that part is not shown in it are shown in Table one.
The data of each embodiment of table one
High-density lipoprotein cholesterol is in the above way measured, response curve has been obtained, Fig. 2, the Fig. 3 listed from the present invention
It can be seen that within 60s in the response curve of Fig. 4, dominant wavelength, commplementary wave length fluctuate, linear after delay 60s to close
System is good, i.e. desirable absorbance after 60s~90s, then reads next absorbance after reacting 60s, calculates mean light absorbency and becomes
The concentration of high-density lipoprotein cholesterol can be calculated after rate, in total 2~3min of time-consuming.
It can also be seen that within 60s from Fig. 2, Fig. 3 and Fig. 4, dominant wavelength, commplementary wave length fluctuate, but main wave
Linear relationship of the length-commplementary wave length in entire reaction period is good, illustrates that setting commplementary wave length has at 700~800nm and disappears
Except the effect of serum background interference, the accuracy of detection is improved.
It is seen in fig. 1, that the prior art measures high-density lipoprotein cholesterol using end-point method, in obtained response curve
It has been shown that, in 5min, terminal is not fully achieved still for reaction, and linear relationship is bad, and detection time need to be greater than 5min；The present invention
Compared with prior art, test period is shortened, the accuracy of test efficiency and testing result is improved.
In conclusion the present invention uses rate determination method, measurement is the rate reacted, linear phase during the reaction
It is read, is different from end assay method, the interference of sample background can be excluded, improve the accuracy of result；The present invention adopts
With rate determination method, delay time is short, and the reading duration is also very short, and the entire reaction time is made to shorten to 2 by 5 original~10min
~3min, improves test efficiency；Operation of the present invention is easy, and instrument parameter setting is simple, and it is solid to reduce high-density lipoprotein gallbladder
The detection technique difficulty of alcohol；The present invention also sets up commplementary wave length in addition to dominant wavelength, and high density is measured at 700~800nm of commplementary wave length
The concentration of lipoprotein cholesterol, is detected using dual wavelength, can be eliminated the physical disturbances such as the noise of sample background, also can
Enough eliminate other specificity interference and nonspecific interference；Detection kit of the invention can be used in full-automatic biochemical analysis
Instrument, semi-automatic biochemical analyzer etc., it is applied widely, while can directly use on the machine, it no longer needs to by other operations such as dilution.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe
It knows those skilled in the art and makes various corresponding changes and modifications, but these corresponding changes and change in accordance with the present invention
Shape all should fall within the scope of protection of the appended claims of the present invention.
Claims (10)
1. a kind of detection method of high-density lipoprotein cholesterol, which is characterized in that the detection method uses rate determination method,
500~546nm of dominant wavelength, at 500~546nm of dominant wavelength, the increase rate and sample of absorbance in reaction system are set
The activity of middle-high density lipoprotein cholesterol is directly proportional, postpones 60~90s, and the absorbance for then measuring 60~150s increases speed
Rate obtains the concentration of high-density lipoprotein cholesterol；
Detecting step is as follows:
A takes blank tube, standard pipe and sample tube, is all separately added into enzymatic reagent 1 in three kinds of pipes, then blank tube, standard pipe and
Corresponding distilled water, titer and the sample that same volume is added, is incubated for 3~5min in sample tube after mixing；Again into three kinds of pipes
It is all separately added into enzymatic reagent 2, mixes, obtains reaction solution；
After reaction solution described in B postpones 60~90s, absorbance is read at 500~546nm of dominant wavelength, reads suction again after reacting 60s
Luminosity calculates mean light absorbency change rate, obtain blank tube, in standard pipe and sample tube reaction solution absorbance per minute variation
Rate；
The concentration of C calculating high-density lipoprotein cholesterol.
2. a kind of detection method of high-density lipoprotein cholesterol according to claim 1, which is characterized in that the enzyme examination
Agent 1 includes cholesterol oxidase and peroxidase, and the enzyme activity of the cholesterol oxidase is 1.4~1.8KU/L, the mistake
The enzyme activity of oxide enzyme is 1.8~2.2KU/L.
3. a kind of detection method of high-density lipoprotein cholesterol according to claim 2, which is characterized in that the enzyme examination
Agent 2 includes cholesteryl esterase, and the enzyme activity of the cholesteryl esterase is 1.8~2.2KU/L.
4. a kind of detection method of high-density lipoprotein cholesterol according to claim 3, which is characterized in that the enzyme examination
The volume ratio of agent 1 and the enzymatic reagent 2 is 2.8~3.3:1.
5. a kind of detection method of high-density lipoprotein cholesterol according to claim 1, which is characterized in that the enzyme examination
The volume ratio of the total volume of agent 1 and enzymatic reagent 2 and the sample is 100~120:1.
6. a kind of detection method of high-density lipoprotein cholesterol according to claim 1, which is characterized in that the detection
Method also sets up 700~800nm of commplementary wave length.
7. a kind of detection method of high-density lipoprotein cholesterol according to claim 1, which is characterized in that the standard
The concentration of liquid is 2~4mmol/L.
8. a kind of detection kit for detection method described in claim 1, which is characterized in that the detection kit packet
Include enzymatic reagent 1 and enzymatic reagent 2, the volume ratio of the enzymatic reagent 1 and the enzymatic reagent 2 is 2.8~3.3:1.
9. a kind of detection kit according to claim 8, which is characterized in that the enzymatic reagent 1 includes cholesterol oxidation
Enzyme and peroxidase, the enzyme activity of the cholesterol oxidase are 1.4~1.8KU/L, the enzyme activity of the peroxidase
For 1.8~2.2KU/L.
10. a kind of detection kit according to claim 8, which is characterized in that the enzymatic reagent 2 includes cholesterol ester
Enzyme, the enzyme activity of the cholesteryl esterase are 1.8~2.2KU/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811487662.0A CN109580511A (en) | 2018-12-06 | 2018-12-06 | A kind of detection method and detection kit of high-density lipoprotein cholesterol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811487662.0A CN109580511A (en) | 2018-12-06 | 2018-12-06 | A kind of detection method and detection kit of high-density lipoprotein cholesterol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109580511A true CN109580511A (en) | 2019-04-05 |
Family
ID=65927574
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811487662.0A Pending CN109580511A (en) | 2018-12-06 | 2018-12-06 | A kind of detection method and detection kit of high-density lipoprotein cholesterol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109580511A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888862A (en) * | 2005-06-29 | 2007-01-03 | 中生北控生物科技股份有限公司 | Low-density lipoprotein cholesterol quantitative determining method reagent and reagent kit |
| CN1888863A (en) * | 2005-06-29 | 2007-01-03 | 中生北控生物科技股份有限公司 | High-density lipoprotein cholesterol quantitative determining method, reagent and reagent kit |
| WO2007007392A1 (en) * | 2005-07-11 | 2007-01-18 | Wako Pure Chemical Industries, Ltd. | Novel polymer and method of measuring cholesterol therewith |
| CN101024149A (en) * | 2007-01-12 | 2007-08-29 | 中国科学院上海有机化学研究所 | Blood plasma lipide component adsorbing separation polymer porous film material, its preparing and use |
| CN101058058A (en) * | 2007-05-23 | 2007-10-24 | 中国科学院上海有机化学研究所 | Surface fixed taurine ligand porous membrane material, preparation method and its application in blood fat adsorption separating |
| CN103122372A (en) * | 2004-03-31 | 2013-05-29 | 电化生研株式会社 | Method of multiquantification for cholesterol of low-density lipoprotein |
| CN104995310A (en) * | 2012-12-10 | 2015-10-21 | 赛拉诺斯股份有限公司 | Rapid, low-sample-volume cholesterol and triglyceride assays |
| CN107449748A (en) * | 2017-08-10 | 2017-12-08 | 威特曼生物科技（南京）有限公司 | HDL-C detection kit and its application method |
| CN107561297A (en) * | 2017-08-25 | 2018-01-09 | 美康生物科技股份有限公司 | Reagent for the detection of blood fat parting |
| CN108690869A (en) * | 2017-04-05 | 2018-10-23 | 广州市伊川生物科技有限公司 | A kind of high-density lipoprotein cholesterol detection kit and its detection method |
-
2018
- 2018-12-06 CN CN201811487662.0A patent/CN109580511A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122372A (en) * | 2004-03-31 | 2013-05-29 | 电化生研株式会社 | Method of multiquantification for cholesterol of low-density lipoprotein |
| CN1888862A (en) * | 2005-06-29 | 2007-01-03 | 中生北控生物科技股份有限公司 | Low-density lipoprotein cholesterol quantitative determining method reagent and reagent kit |
| CN1888863A (en) * | 2005-06-29 | 2007-01-03 | 中生北控生物科技股份有限公司 | High-density lipoprotein cholesterol quantitative determining method, reagent and reagent kit |
| WO2007007392A1 (en) * | 2005-07-11 | 2007-01-18 | Wako Pure Chemical Industries, Ltd. | Novel polymer and method of measuring cholesterol therewith |
| CN101024149A (en) * | 2007-01-12 | 2007-08-29 | 中国科学院上海有机化学研究所 | Blood plasma lipide component adsorbing separation polymer porous film material, its preparing and use |
| CN101058058A (en) * | 2007-05-23 | 2007-10-24 | 中国科学院上海有机化学研究所 | Surface fixed taurine ligand porous membrane material, preparation method and its application in blood fat adsorption separating |
| CN104995310A (en) * | 2012-12-10 | 2015-10-21 | 赛拉诺斯股份有限公司 | Rapid, low-sample-volume cholesterol and triglyceride assays |
| CN108690869A (en) * | 2017-04-05 | 2018-10-23 | 广州市伊川生物科技有限公司 | A kind of high-density lipoprotein cholesterol detection kit and its detection method |
| CN107449748A (en) * | 2017-08-10 | 2017-12-08 | 威特曼生物科技（南京）有限公司 | HDL-C detection kit and its application method |
| CN107561297A (en) * | 2017-08-25 | 2018-01-09 | 美康生物科技股份有限公司 | Reagent for the detection of blood fat parting |
Non-Patent Citations (2)
| Title |
|---|
| 郑铁生，鄢盛恺主编；谢圣高等副主编: "《临床生物化学检验》", 31 August 2015, 北京：中国医药科技出版社 * |
| 韩志钧等主编: "《临床化学常用项目自动分析法 第3版》", 31 August 2005, 沈阳：辽宁科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105296597B (en) | Kit for detecting high density lipoprotein cholesterol content | |
| CN104198473B (en) | A kind of uric acid detection kit of stabilization | |
| CN106383116B (en) | A kind of kit detecting high-density lipoprotein cholesterol | |
| CN103276052B (en) | A kind of Urea nitrogen detection reagent | |
| CN105241873B (en) | A kind of fat enzyme detection kit | |
| CN109239059B (en) | Glycated serum protein assay kit and preparation method and application thereof | |
| CN103266166B (en) | Glucose detecting reagent | |
| CN105388146A (en) | Kit for simultaneously detecting sodium, creatinine and microalbumin in urine | |
| CN107449748A (en) | HDL-C detection kit and its application method | |
| CN104198474A (en) | Detection kit for measuring content of lipase in serum by colorimetric method | |
| CN105652021A (en) | Reagent, method and kit for measuring small-and-dense lipoprotein | |
| Nauck et al. | Homogeneous assay for direct determination of high-density lipoprotein cholesterol evaluated | |
| CN110564810A (en) | High-performance small and dense low-density lipoprotein cholesterol detection kit | |
| CN104198407A (en) | Detection kit for detecting content of beta-hydroxybutyrate in serum by adopting stable enzymatic method | |
| CN103266165B (en) | Amylase detection reagent | |
| CN106367471A (en) | Kit for measuring total cholesterol and method | |
| CN109580511A (en) | A kind of detection method and detection kit of high-density lipoprotein cholesterol | |
| CN107153044A (en) | The homocysteine kit and its detection method of a kind of modified form | |
| CN110736846B (en) | Small and dense lipoprotein determination reagent, method and kit | |
| US11747328B2 (en) | Method for measuring the plasma concentration of an analyte directly on a whole blood sample | |
| CN106324234B (en) | Neu 5 Ac aldolase of modification and its preparation method and application | |
| JPH0218840B2 (en) | ||
| CN103837487A (en) | Uric acid detection method and detection kit | |
| CN114441516A (en) | Uric acid detection kit and preparation method thereof | |
| CN107764877B (en) | A kind of highly sensitive serum tolal bile acid electrochemical detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |
|
| RJ01 | Rejection of invention patent application after publication |