CN109576257B - 一种局部光热效应的酶催化剂及其制备方法 - Google Patents
一种局部光热效应的酶催化剂及其制备方法 Download PDFInfo
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Abstract
本发明涉及一种局部光热效应的酶催化剂及其制备方法。本发明提出一种催化剂,由光热材料、聚多巴胺及酶组成,经过光热材料将聚多巴胺修饰到石墨烯表面,最后将酶通过聚多巴胺固定在光热材料上形成催化剂。本发明具有可以促进局部发热以达到催化反应的目的,且本发明的制备方法酶适用性广,操作简便,易于工业化,具有良好的应用前景。
Description
技术领域
本发明涉及催化剂领域,尤其涉及一种局部光热效应的酶催化剂及其制备方法。
背景技术
化学反应中存在旧键的断裂和新键的形成,其过程中涉及到反应能的变化。在化学反应中加入催化剂,能够降低反应能,使化学反应更快进行。催化剂分为天然催化剂和人工合成催化剂。无论采用人工合成催化剂还是采用天然催化剂,在催化反应中,经常需要通过加热来提高反应物分子的能级,使其能够更轻易地越过能量壁垒,发生化学反应。但是,对于一些热稳定性差的反应物分子,或者加热引起较多副反应的反应过程,对反应体系进行整体加热往往不是理想的选择。如果能够实现催化剂表面区域的局部加热,而不改变反应体系整体的温度,即只有当反应物分子接近催化剂时才会被加热而发生催化反应,这种方法将非常有利于热稳定性差的反应物分子发生催化反应,也有利于加热会引起较多副反应发生的反应过程。
酶作为一类由生物体产生的天然催化剂,相比传统的人工合成催化剂,具有高效、专一、反应条件温和等优点,在医药合成、生物传感器等领域应用广泛。游离酶的应用仍存在很多问题,例如在储存及反应过程中酶易失活或变性而导致较差的操作稳定性及重复使用性;另外,酶促反应后酶与底物和产物不易分离,导致产品纯度不高且增加了生产成本。为了解决酶存在的问题,人们将酶固定于不同载体材料中,如多孔载体材料(介孔硅,介孔TiO2等)、含多电子基团的载体(水滑石,多糖类,碳材料等)。
发明专利申请CN104569099A的中国专利申请公开了一种快速测酚电极及其制备方法,将1-氨基芘(1-AP)功能化的还原氧化石墨烯(rGO)与戊二醛(GA)一端的醛基交联,然后将酪氨酸酶(Tyr)交联到GA另一端的醛基上,得到rGO-1-AP-GA-Tyr酶液。最后将酶液滴涂到丝网印刷电极(SPE)上,得到修饰电极,此发明的酶电极用于化学分析和环境监测领域,具有成本低,操作简便,响应速度快,灵敏度高,检测限低,选择性好等特点。但其制备的产品不能引起自然光下局部发热,无法达到催化反应物的目的。
发明专利申请CN106542568A公开了一种固定化酶、固定化酶载体及其制备方法。本发明申请中固定化酶载体包括氧化石墨烯及修饰于所述氧化石墨烯表面的含有多电子基团的无机纳米颗粒;固定化酶载体是由溶剂中的修饰剂与表面活性剂充分反应形成无机纳米颗粒并修饰于氧化石墨烯表面而制得,固定化酶则包括固定化酶载体及固定于固定化酶载体上的脂肪酶。本发明固定化酶载体一方面其表面具有大量的含氧官能团与酶结合率较高且能够将酶牢固的固定在载体表面,提高了酶的操作稳定性;另一方面具有较大比表面积,为固定化酶在催化过程中提供了充足的催化界面环境及质量转移;另外,固定化酶载体抑制了氧化石墨烯在反复使用过程中的团聚,减少了酶被石墨层覆盖,提高了酶的重复使用性能。但其制备方法适合修饰的酶的种类少,具有一定的局限性,且未公开是否促进局部升温。
因此,需要一种酶适用性范围广、可以促进局部升温已达到催化化学反应目的的催化剂。所得催化剂可以适用于催化热稳定性差的反应分子的催化反应,以及加热引起较多副反应发生的反应过程,在医药合成、生物传感器等领域有很好的应用前景。
发明内容
为了解决现有技术中酶适用性差、不能促进局部加热等问题,本发明提供一种局部光热效应的酶催化剂及其制备方法。
为实现上述目的,本发明提供一种局部光热效应的酶催化剂,其特征在于,由光热材料、聚多巴胺及酶组成。
进一步地,所述光热材料为四氧化三铁磁性石墨烯或氧化石墨烯中的一种。
进一步地,所述催化剂的结构为:酶通过聚多巴胺固定在光热材料表面。
进一步地,所述催化剂,光热材料与聚多巴胺的质量比为0.00001-10:1。
更进一步地,所述催化剂,光热材料与聚多巴胺的质量比为0.22-1:1。
更进一步地,所述催化剂,光热材料与聚多巴胺的质量比为0.22:1。
更进一步地,所述催化剂,光热材料与聚多巴胺的质量比为3:1。
进一步地,所述催化剂,所述酶与光热材料的质量比为0.00001-1:1。
更进一步地,所述催化剂,所述酶与光热材料的质量比为0.22-0.43:1。
更进一步地,所述催化剂,所述酶与光热材料的质量比为0.22:1。
更进一步地,所述催化剂,所述酶与光热材料的质量比为0.43:1。
进一步地,所述催化剂,酶为细胞色素C、细胞色素P450、辣根过氧化物酶、乙醇脱氢酶、脂肪酶、乙酰胆碱酯酶、漆酶、绿色荧光蛋白、葡萄糖脱氢酶、葡萄糖氧化酶、胰蛋白酶、枯草杆菌蛋白酶、碳酸酐酶、乙醇脱氢酶、蔗糖酶、南极假丝酵母脂肪酶、有机磷水解酶、3α-羟类固醇脱氢酶、黄递酶、尿酸酶、乳酸脱氢酶、过氧化氢酶中的任一种或几种。
进一步地,所述酶的分子量为5-300kDa
一种局部光热效应的酶催化剂的制备方法,包括以下步骤:
(1)将光热材料分散于溶剂中,超声,得到混合物A;
(2)将聚多巴胺溶于溶剂中,将聚多巴胺溶液注入混合物A中,振荡混合反应,利用磁铁吸附,得固体B,去离子水冲洗固体B,然后将B超声分散于溶剂中得到混合物C;
(3)将酶溶于溶剂中,离心,滤膜过滤,得到酶溶液,将酶溶液与C混合,振荡混合反应,然后用磁铁吸附,得固体D,去离子水冲洗固体D。
进一步地,所述制备方法中,所述光热材料分散液浓度为0.5-5mg/mL。
进一步地,所述制备方法中,所述聚多巴胺浓度为0.5-5mg/mL。
进一步地,所述制备方法中,所述酶浓度为0.1-2mg/mL。
进一步地,所述制备方法中,所述步骤(1)、步骤(2)中所述溶剂为水、Tris溶液、HCl溶液、甲醇、磷酸盐溶液、二甲基甲酰胺、乙醇、乙二醇、异丙醇、叔丁醇、丙三醇、二甲基亚砜、乙腈、TritonX-100溶液或丙酮中的一种或几种的混合物。
进一步地,所述制备方法中,所述步骤(1)中,溶剂体积为30ml。
进一步地,所述制备方法中,所述步骤(1)中,超声时间为0.5-1h。
进一步地,所述制备方法中,所述步骤(2)中,溶剂体积为10-100ml。
更进一步地,所述制备方法中,所述步骤(2)中,溶剂体积为30ml。
进一步地,所述制备方法中,所述步骤(2)中,振荡温度为4℃-50℃。
进一步地,所述制备方法中,所述步骤(2)中,振荡时间为0.01-5h。
进一步地,所述制备方法中,所述步骤(2)中,固体B冲洗次数为3-5次。
进一步地,所述制备方法中,所述步骤(3)中,溶剂为PB缓冲溶液。
进一步地,所述制备方法中,所述步骤(3)中,溶剂体积为10-100ml。
更进一步地,所述制备方法中,所述步骤(3)中,溶剂体积为30ml。
进一步地,所述制备方法中,所述步骤(3)中,离心速度为9000-11000r/min。
进一步地,所述制备方法中,所述步骤(3)中,离心时间为5-10min。
进一步地,所述制备方法中,所述步骤(3)中,振荡温度为4℃-50℃。
进一步地,所述制备方法中,所述步骤(3)中,固体D冲洗次数为3-5次。
进一步地,所述制备方法中,包括以下步骤:
(1)将光热材料分散于10-100ml溶剂中,超声0.5-1h,得到混合物A;
(2)将聚多巴胺溶于10-100ml溶剂中,将聚多巴胺溶液注入混合物A中,4℃-50℃下振荡混合反应0.01-5h,利用磁铁吸附,得固体B,去离子水冲洗固体B 3-5次,然后将B超声分散于10-100ml溶剂中得到混合物C;
(3)将酶溶于10-100ml溶剂中,9000-11000r/min下离心5-10min,滤膜过滤,得到酶溶液,将酶溶液与C混合,4℃-50℃下振荡1-3h,混合反应,然后用磁铁吸附,得固体D,去离子水冲洗固体D 3-5次,得到催化剂。
与现有技术相比,本发明提供一种局部光热效应的酶催化剂及其制备方法,通过选择合适的媒介,成功的将酶与石墨烯结合起来,在催化过程中起到局部催化的效果;通过确定聚多巴胺与光热材料的比例、酶与光热材料的比例,使得酶与光热材料的结合更牢固;明确了合适的制备方法、最优的制备条件,得到催化效果良好的酶催化剂;
综上所述,具有以下优点及有益效果:
(1)可以局部促进发热,实现局部催化反应的目的;
(2)可适用的酶种类多,酶适用范围广;
(3)制备方法简单,条件温和,易于工业化。
附图说明
图1是实施例1中为四氧化三铁磁性石墨烯的复合材料(下称Fe3O4@rGO)的扫描电镜照片。
图2是实施例1中Fe3O4@rGO的透射电镜照片。
图3是实施例1中为聚多巴胺、四氧化三铁磁性石墨烯的复合材料(下称PDA-Fe3O4@rGO)的扫描电镜照片。
图4是实施例1中PDA-Fe3O4@rGO的透射电镜照片。
图5是实施例1中为南极假丝酵母脂肪酶、聚多巴胺、四氧化三铁磁性石墨烯的复合材料(下称CALB-PDA-Fe3O4@rGO)的扫描电镜照片。
图6是实施例1中CALB-PDA-Fe3O4@rGO酶催化剂的透射电镜照片。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白,以下结合附图及实施例,对本发明进行进一步详细说明。应当理解,此处所描述的具体实施例仅仅用以解释本发明,并不用于限定本发明。
实施例1
制备南极假丝酵母脂肪酶-光热材料的酶催化剂
(1)将10mg四氧化三铁磁性石墨烯(购自于南京先丰纳米材料科技有限公司,编号XF018,下文所述四氧化三铁磁性石墨烯均为购自于南京先丰纳米材料科技有限公司,编号XF018)分散于20ml,20mM,pH=8.0的Tris-HCl缓冲液中,超声处理0.5h,得到混合物A,扫描电镜和透射电镜图分别见图1和图2;
(2)将1mg聚多巴胺溶于2ml去离子水中得聚多巴胺溶液,将聚多巴胺溶液注入混合物A中,50℃振荡混合反应0.01h,利用磁铁吸附,得固体B,去离子水冲洗固体B 3次,然后将B分散于10ml去离子水中得到混合物C,固体B的扫描电镜和透射电镜分别见图3和图4;
(3)将4.3mg南极假丝酵母脂肪酶溶于43ml,50mM,pH=7.0的PB缓冲液中,在11000r/min转速下离心5min,滤膜过滤,得到南极假丝酵母脂肪酶溶液,将南极假丝酵母脂肪酶溶液与C混合,4℃下振荡混合反应3h,然后用磁铁吸附,得固体D,去离子水冲洗固体D5次,固体D的扫描电镜和透射电镜分别见图5和图6。
实施例2
制备辣根过氧化物酶-光热材料的酶催化剂
(1)将1mg氧化石墨烯分散于0.2ml,20mM,pH=7.0的Tris-HCl缓冲液中,超声处理1h,得到混合物A;
(2)将1mg聚多巴胺溶于0.2ml去离子水中得聚多巴胺溶液,将聚多巴胺溶液注入混合物A中,4℃振荡混合反应1h,利用磁铁吸附,得固体B,去离子水冲洗固体B 5次,然后将B分散于100ml去离子水中得到混合物C;
(3)将0.22mg辣根过氧化物酶溶于0.11ml,50mM,pH=7.0的PB缓冲液中,在9000r/min转速下离心10min,滤膜过滤,得到辣根过氧化物酶溶液,将辣根过氧化物酶溶液与C混合,50℃下振荡混合反应0.01h,然后用磁铁吸附,得固体D,去离子水冲洗固体D 3次。
实施例3
制备有机磷水解酶-光热材料的酶催化剂
(1)将22mg氧化石墨烯分散于30ml,20mM,pH=9.0的Tris-HCl缓冲液中,超声处理1h,得到混合物A;
(2)将100mg聚多巴胺溶于100ml去离子水中得聚多巴胺溶液,将聚多巴胺溶液注入混合物A中,25℃振荡混合反应2h,利用磁铁吸附,得固体B,去离子水冲洗固体B 4次,然后将B分散于50ml去离子水中得到混合物C;
(3)将0.00022mg有机磷水解酶溶于0.00055ml,50mM,pH=9.0的PB缓冲液中,在10000r/min转速下离心10min,滤膜过滤,得到有机磷水解酶溶液,将有机磷水解酶溶液与C混合,20℃下振荡混合反应2h,然后用磁铁吸附,得固体D,去离子水冲洗固体D 4次。
实施例4
制备尿酸酶-光热材料的酶催化剂
(1)将0.01mg四氧化三铁磁性石墨烯分散于0.02ml,20mM,pH=6.5的Tris-HCl缓冲液中,超声处理1h,得到混合物A;
(2)将1000mg聚多巴胺溶于200ml去离子水中得聚多巴胺溶液,将聚多巴胺溶液注入混合物A中,25℃振荡混合反应5h,利用磁铁吸附,得固体B,去离子水冲洗固体B 4次,然后将B分散于100ml去离子水中得到混合物C;
(3)将0.01mg尿酸酶溶于0.05ml,40mM,pH=6.5的PB缓冲液中,在10000r/min转速下离心20min,滤膜过滤,得到尿酸酶溶液,将尿酸酶溶液与C混合,20℃下振荡混合反应5h,然后用磁铁吸附,得固体D,去离子水冲洗固体D 3次。
对比例1
与实施例1相比,聚多巴胺与光热材料的比例超出范围。
制备南极假丝酵母脂肪酶-还原氧化石墨烯的酶催化剂
(1)将10mg四氧化三铁磁性石墨烯分散于20ml,20mM,pH=8.0的Tris-HCl缓冲液中,超声处理0.5h,得到混合物A;
(2)将15mg聚多巴胺溶于30ml去离子水中得聚多巴胺溶液,将聚多巴胺溶液注入混合物A中,50℃振荡混合反应0.01h,利用磁铁吸附,得固体B,去离子水冲洗固体B 3次,然后将B分散于10ml去离子水中得到混合物C;
(3)将4.3mg南极假丝酵母脂肪酶溶于43ml,50mM,pH=7.0的PB缓冲液中,在11000r/min转速下离心5min,滤膜过滤,得到南极假丝酵母脂肪酶溶液,将南极假丝酵母脂肪酶溶液与C混合,4℃下振荡混合反应3h,然后用磁铁吸附,得固体D,去离子水冲洗固体D5次。
对比例2
与实施例1相比,改变了制备方法,合并步骤(1)(2),并省略对固体B的清洗过程。
制备南极假丝酵母脂肪酶-还原氧化石墨烯的酶催化剂
(1)将10mg四氧化三铁磁性石墨烯、1mg聚多巴胺分散于20ml,20mM,pH=8.0的Tris-HCl缓冲液中,超声处理0.5h,振荡混合0.01h,得到混合物A;
(2)将混合物A,利用磁铁吸附,得固体B,将B分散于10ml去离子水中得到混合物C;
(3)将4.3mg南极假丝酵母脂肪酶溶于43ml,50mM,pH=7.0的PB缓冲液中,在11000r/min转速下离心5min,滤膜过滤,得到南极假丝酵母脂肪酶溶液,将南极假丝酵母脂肪酶溶液与C混合,4℃下振荡混合反应3h,然后用磁铁吸附,得固体D,去离子水冲洗固体D5次。
对比例3
与实施例1相比,酶的浓度和离心转速都超过了范围。
制备南极假丝酵母脂肪酶-还原氧化石墨烯的酶催化剂
(1)将10mg四氧化三铁磁性石墨烯分散于20ml,20mM,pH=8.0的Tris-HCl缓冲液中,超声处理0.5h,得到混合物A;
(2)将1mg聚多巴胺溶于2ml去离子水中得聚多巴胺溶液,将聚多巴胺溶液注入混合物A中,50℃振荡混合反应0.01h,利用磁铁吸附,得固体B,去离子水冲洗固体B 3次,然后将B分散于10ml去离子水中得到混合物C;
(3)将4.3mg南极假丝酵母脂肪酶溶于50ml,50mM,pH=7.0的PB缓冲液中,在15000r/min转速下离心5min,滤膜过滤,得到南极假丝酵母脂肪酶溶液,将南极假丝酵母脂肪酶溶液与C混合,4℃下振荡混合反应3h,然后用磁铁吸附,得固体D,去离子水冲洗固体D5次。
实验例1
将20μL实施例1制备的复合催化剂分散液滴加到5mL含有聚乙二醇辛基苯基醚(Triton X-100)的50mM,pH=6.5的磷酸盐缓冲液(Triton质量分数为1.25%)中,保持溶液28℃,使用太阳模拟器模拟太阳光源对反应体系进行照射,光源距离反应容器90cm,此时光照强度约为300mW/cm2。在保持光照的条件下,向反应体系边振荡边缓慢滴加200μL对硝基苯酚棕榈酸酯(p-NPP)的丙酮溶液。
反应15分钟后,在紫外可见分光光度计上观察并记录吸光度值,与底物完全反应后的吸光度值相比较,计算底物转化率为4.2%。
实验例2
将20μL对比例1制备的复合催化剂分散液滴加到5mL含有聚乙二醇辛基苯基醚(Triton X-100)的50mM,pH=6.5的磷酸盐缓冲液(Triton质量分数为1.25%)中,保持溶液28℃,使用太阳模拟器模拟太阳光源对反应体系进行照射,光源距离反应容器90cm,此时光照强度约为300mW/cm2。在保持光照的条件下,向反应体系边振荡边缓慢滴加200μL对硝基苯酚棕榈酸酯(p-NPP)的丙酮溶液。
反应15分钟后,在紫外可见分光光度计上观察并记录吸光度值,与底物完全反应后的吸光度值相比较,计算底物转化率为3.4%。
实验例3
将20μL对比例2制备的复合催化剂分散液滴加到5mL含有聚乙二醇辛基苯基醚(Triton X-100)的50mM,pH=6.5的磷酸盐缓冲液(Triton质量分数为1.25%)中,保持溶液28℃,使用太阳模拟器模拟太阳光源对反应体系进行照射,光源距离反应容器90cm,此时光照强度约为300mW/cm2。在保持光照的条件下,向反应体系边振荡边缓慢滴加200μL对硝基苯酚棕榈酸酯(p-NPP)的丙酮溶液。
反应15分钟后,在紫外可见分光光度计上观察并记录吸光度值,与底物完全反应后的吸光度值相比较,计算底物转化率为3.5%。
实验例4
将20μL对比例3制备的复合催化剂分散液滴加到5mL含有聚乙二醇辛基苯基醚(Triton X-100)的50mM,pH=6.5的磷酸盐缓冲液(Triton质量分数为1.25%)中,保持溶液28℃,使用太阳模拟器模拟太阳光源对反应体系进行照射,光源距离反应容器90cm,此时光照强度约为300mW/cm2。在保持光照的条件下,向反应体系边振荡边缓慢滴加200μL对硝基苯酚棕榈酸酯(p-NPP)的丙酮溶液。
反应15分钟后,在紫外可见分光光度计上观察并记录吸光度值,与底物完全反应后的吸光度值相比较,计算底物转化率为3.4%。
实验例5
将20μL南极假丝酵母脂肪酶分散液滴加到5mL含有聚乙二醇辛基苯基醚(TritonX-100)的50mM,pH=6.5的磷酸盐缓冲液(Triton质量分数为1.25%)中,保持溶液28℃,使用太阳模拟器模拟太阳光源对反应体系进行照射,光源距离反应容器90cm,此时光照强度约为300mW/cm2。在保持光照的条件下,向反应体系边振荡边缓慢滴加200μL对硝基苯酚棕榈酸酯(p-NPP)的丙酮溶液。
反应15分钟后,在紫外可见分光光度计上观察并记录吸光度值,与底物完全反应后的吸光度值相比较,计算底物转化率为3.1%。
实验例6
将20μL南极假丝酵母脂肪酶和分散的四氧化三铁磁性石墨烯分散液滴加到5mL含有聚乙二醇辛基苯基醚(Triton X-100)的50mM,pH=6.5的磷酸盐缓冲液(Triton质量分数为1.25%)中,保持溶液28℃,使用太阳模拟器模拟太阳光源对反应体系进行照射,光源距离反应容器90cm,此时光照强度约为300mW/cm2。在保持光照的条件下,向反应体系边振荡边缓慢滴加200μL对硝基苯酚棕榈酸酯(p-NPP)的丙酮溶液。
反应15分钟后,在紫外可见分光光度计上观察并记录吸光度值,与底物完全反应后的吸光度值相比较,计算底物转化率为3.4%。
南极假丝酵母脂肪酶的最适温度为45~55℃,在300mW/cm2的模拟光源光照下,复合催化剂的催化能力要比四氧化三铁磁性石墨烯与南极假丝酵母脂肪酶分散的情况高出23%,比仅有天然脂肪酶存在的情况高出35%,可以认为是由于酶催化剂表面局部升温导致催化效力提高,复合催化剂局部发生了光热效应,催化了反应进行,具有良好的催化效果,具有高效的优势。
Claims (9)
1.一种局部光热效应的酶催化剂,其特征在于,由光热材料、聚多巴胺及酶组成;
所述光热材料为四氧化三铁磁性石墨烯;所述催化剂结构为:酶通过聚多巴胺固定在光热材料表面;
所述光热材料与聚多巴胺的质量比为0.22-1:1或3:1。
2.根据权利要求1所述的催化剂,其特征在于,光热材料与聚多巴胺的质量比为3:1或0.22:1。
3.根据权利要求1所述的催化剂,其特征在于,所述酶与光热材料的质量比为0.00001-1:1。
4.根据权利要求1所述的催化剂,其特征在于,所述酶与光热材料的质量比为0.22-0.43:1。
5.根据权利要求1所述的催化剂,其特征在于,所述酶为细胞色素C、细胞色素P450、辣根过氧化物酶、乙醇脱氢酶、脂肪酶、乙酰胆碱酯酶、漆酶、绿色荧光蛋白、葡萄糖脱氢酶、葡萄糖氧化酶、胰蛋白酶、枯草杆菌蛋白酶、碳酸酐酶、乙醇脱氢酶、蔗糖酶、南极假丝酵母脂肪酶、有机磷水解酶、3α-羟类固醇脱氢酶、黄递酶、尿酸酶、乳酸脱氢酶、过氧化氢酶中的任一种或几种;所述酶的分子量为5-300kDa。
6.制备权利要求1所述的催化剂的制备方法,其特征在于,包括以下步骤:
(1)将光热材料分散于溶剂中,得到混合物A;
(2)将聚多巴胺溶于溶剂中得聚多巴胺溶液,将聚多巴胺溶液注入混合物A中,振荡混合反应,利用磁铁吸附,得固体B,去离子水冲洗固体B,然后将B分散于溶剂中得到混合物C;
(3)将酶溶于溶剂中,离心,滤膜过滤,得到酶溶液,将酶溶液与C混合,振荡混合反应,然后用磁铁吸附,得固体D,去离子水冲洗固体D。
7.根据权利要求6所述的制备方法,其特征在于,所述光热材料浓度为0.5-5mg/mL;所述聚多巴胺浓度为0.5-5mg/mL;所述酶浓度为0.1-2mg/mL。
8.根据权利要求6所述的制备方法,其特征在于,所述步骤(1)、步骤(2)中所述溶剂为水、Tris溶液、HCl溶液、甲醇、磷酸盐溶液、二甲基甲酰胺、乙醇、乙二醇、异丙醇、叔丁醇、丙三醇、二甲基亚砜、乙腈、TritonX-100溶液或丙酮中的一种或几种的混合物。
9.根据权利要求6所述的制备方法,其特征在于,包括以下步骤:
(1)将光热材料分散于溶剂中,超声0.5-1h,得到混合物A;
(2)将聚多巴胺溶于溶剂中,将聚多巴胺溶液注入混合物A中,4℃-50℃下振荡混合反应0.01-5h,利用磁铁吸附,得固体B,去离子水冲洗固体B 3-5次,然后将B超声分散于溶剂中得到混合物C;
(3)将酶溶于溶剂中,9000-11000r/min下离心5-10min,滤膜过滤,得到酶溶液,将酶溶液与C混合,4℃-50℃下振荡1-3h,混合反应,然后用磁铁吸附,得固体D,去离子水冲洗固体D 3-5次,得到催化剂。
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