CN109568651B - Long-acting liquid dressing - Google Patents
Long-acting liquid dressing Download PDFInfo
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/62—Encapsulated active agents, e.g. emulsified droplets
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Abstract
The invention belongs to the technical field of medical material production, and particularly discloses a long-acting liquid dressing, wherein the long-acting hydrogel dressing is a hydrogel microcapsule dressing prepared from nano-silver, sodium hydroxy cellulose, bacterial cellulose and modified chitosan, and the gel matrix of the hydrogel is polyvinylpyrrolidone; the microcapsule is prepared from a mixed solution of sodium hydroxy cellulose, bacterial cellulose, modified chitosan and nano-silver, liquid paraffin, an emulsifier and carbodiimide as a cross-linking agent. The nano silver is wrapped in the microcapsule, then the microcapsule is added into the gel matrix to form the hydrogel dressing, the microcapsule gradually swells to release the medicinal components, the controllability of the release of the medicinal components is realized, the sustained-release effect is realized, the medicament can continuously and long-acting act on wounds, and the defects that the medicinal components cannot continuously exert the medicinal effect and the dressing needs to be continuously replaced due to the fact that the traditional dressing directly and completely releases the medicament are avoided.
Description
Technical Field
The invention relates to a medical dressing patch, in particular to a long-acting liquid dressing.
Background
The dressing is an essential material for dressing and treating wounds or wound surfaces. The medical dressing is a medical product which is covered on a wound surface, plays a role of temporarily replacing damaged skin to protect tissues in the continuous healing process of the wound surface, is used as a barrier, avoids the wound surface from being polluted by external environment, and provides an excellent healing and rehabilitation environment. In order to promote the healing of the wound, it is often necessary to add functional components to the dressing, such as antimicrobial components, healing promoting components, hemostatic components, and the like. However, the functional ingredients in the conventional dressing are usually prepared into different forms of dressings by directly using the original state of the ingredients as one of raw materials of the dressing, for example, in the hydrogel dressing, an antibacterial agent and a hemostatic ingredient are added, and the functional ingredients are directly used as the raw materials and added into a gel matrix to prepare the hydrogel dressing. Although the dressing prepared by the traditional method can also exert the antibacterial and healing promoting effects of the functional components, the medicinal components of the dressing are completely and directly released at one time, the duration of the medicinal effect of the medicament is short, the long-acting effects of sterilizing and promoting healing of the wound surface cannot be exerted, and the dressing needs to be continuously replaced, so that the use is inconvenient.
The controlled release of the drug is realized by slowly releasing the drug outwards through a controlled release agent, so that the drug effect is kept constant for a long time, and the controlled release function of the drug can be achieved through a membrane permeation control system and an organism diffusion system. The dressing containing the traditional medicine auxiliary materials is difficult to control release and cannot meet the daily medical requirements, in the known gel manufacturing process, because the reaction mixed solution for forming the gel is in a solution state before polymerization reaction, the gel is required to be contained in a container for polymerization reaction to form a gel block, and then the gel block is cut into the gel dressing with proper thickness and size, so the manufacturing process is complicated.
Disclosure of Invention
The invention mainly aims to provide a long-acting liquid dressing which can slowly prepare nano silver and has long-acting antibacterial performance. In particular to a hydrogel microcapsule dressing prepared from nano-silver, sodium hydroxy cellulose, bacterial cellulose and modified chitosan. The gel matrix of the hydrogel is polyvinylpyrrolidone; the microcapsule is prepared from a mixed solution of sodium hydroxy cellulose, bacterial cellulose, modified chitosan and nano-silver, liquid paraffin, an emulsifier and carbodiimide as a cross-linking agent.
The preparation method of the hydrogel microcapsule comprises the following operation steps:
(1) rinsing wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into an alkali solution for heating treatment, neutralizing by using an acid solution, and then washing the obtained product to be neutral by using deionized water;
(2) adding the bacterial cellulose prepared in the step (1), nano silver, sodium hydroxy cellulose and modified chitosan into an organosilicon quaternary ammonium salt solution, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, adding an emulsifier, and uniformly mixing;
(4) dropwise adding the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, after dropwise adding is finished, adding a cross-linking agent carbodiimide into the emulsion, continuously stirring after finishing dropwise adding, centrifuging to remove an upper oil layer, retaining lower-layer particles, washing, and freeze-drying to obtain the microcapsule;
(5) and (4) preparing a polyvinylpyrrolidone aqueous solution, adding glycerol and the microcapsule prepared in the step (5), uniformly mixing, and performing irradiation treatment to form hydrogel.
The preferred mass ratio of the nano-silver, the sodium hydroxy cellulose, the bacterial cellulose and the modified chitosan is (0.01-0.1): (10-20): (20-50): (10-20), and the further preferred mass ratio is: (0.05-0.1): (15-20): 30-50): 10-15), more preferably 0.1:15:40: 10.
The mass concentration of the organosilicon quaternary ammonium salt in the step (2) is preferably 2-5%.
In the step (3), the emulsifier is preferably a mixture of Tween 80 and span80 according to the proportion of 1 (2-5).
The stirring speed in the step (4) is preferably 1200-1500 r/min.
The mass concentration of the polyvinylpyrrolidone aqueous solution in step (5) is preferably 5 to 10%.
The mass ratio of the glycerol in the step (5), the microcapsule prepared in the step (5) and the polyvinylpyrrolidone aqueous solution is preferably 1 (10-20) to (20-30).
The modified chitosan is preferably chitosan deacetylated product, or modified chitosan or chitosan derivative, more preferably chitosan deacetylation degree of 60-80%, and more preferably chitosan deacetylation degree of 80%.
The long-acting hydrogel dressing is formed by wrapping nano silver in the microcapsule and then adding the microcapsule into the gel matrix, and the dressing is coated on a wound surface in the using process of the dressing, so that the microcapsule gradually swells to release medicinal ingredients under the action of wound surface exudate and body temperature, the controllability of the release of the medicinal ingredients is realized, the dressing has a slow release effect, the medicament can continuously and long-acting act on the wound, and the defect that the medicinal ingredients cannot continuously exert the medicinal effect and the dressing needs to be continuously replaced due to the fact that the traditional dressing directly and completely releases the medicament is avoided.
Detailed Description
The following embodiments are provided to describe the principle and features of the present invention, and the examples are only for explaining the present invention and not for limiting the scope of the present invention.
Example 1
The preparation method of the long-acting liquid dressing comprises the following steps:
(1) rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 40g of bacterial cellulose prepared in the step (1), 50mg of nano-silver, 10g of sodium hydroxy cellulose and 20g of modified chitosan (the deacetylation degree of the chitin is 80%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 5%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:3 to ensure that the mass percentage of tween 80 and span80 is 8 percent, and uniformly mixing;
(4) dropwise adding 150g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 5g of carbodiimide as a cross-linking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 5%, adding glycerol according to the mass ratio of 1:20 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Example 2
The preparation method of the long-acting liquid dressing comprises the following steps:
(1) rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 20g of bacterial cellulose prepared in the step (1), 100mg of nano-silver, 20g of sodium hydroxy cellulose and 15g of modified chitosan (the deacetylation degree of the chitin is 70%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 7%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:3 to ensure that the mass percentage content of tween 80 and span80 is 10 percent, and uniformly mixing;
(4) dropwise adding 120g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 8g of carbodiimide as a crosslinking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing a 10 mass percent polyvinylpyrrolidone aqueous solution, adding glycerol according to the mass ratio of 1:25 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Example 3
The preparation method of the long-acting liquid dressing comprises the following steps:
(1) rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 50g of bacterial cellulose prepared in the step (1), 30mg of nano-silver, 15g of sodium hydroxy cellulose and 10g of modified chitosan (the deacetylation degree of the chitin is 60%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 8%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:5 to ensure that the mass percentage content of tween 80 and span80 is 10 percent, and uniformly mixing;
(4) dropwise adding 180g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 5g of carbodiimide as a cross-linking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 8%, adding glycerol according to the mass ratio of 1:10 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:15 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Example 4
The preparation method of the long-acting liquid dressing comprises the following steps:
(1) rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 30g of bacterial cellulose prepared in the step (1), 80mg of nano-silver, 20g of sodium hydroxy cellulose and 20g of modified chitosan (the deacetylation degree of the chitin is 80%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 10%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:4 to ensure that the mass percentage content of tween 80 and span80 is 10 percent, and uniformly mixing;
(4) dropwise adding 160g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion at the stirring speed of 1200r/min in the dropwise adding process, adding 5g of carbodiimide as a crosslinking agent into the emulsion after dropwise adding, continuously stirring at the stirring speed of 1500r/min, centrifuging to remove an upper oil layer, retaining lower particles, washing, and freeze-drying to obtain microcapsules;
(5) preparing a 10% polyvinylpyrrolidone aqueous solution, adding glycerol according to the mass ratio of 1:10 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Example 5
The preparation method of the long-acting liquid dressing comprises the following steps:
(1) rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 50g of bacterial cellulose prepared in the step (1), 60mg of nano-silver, 10g of sodium hydroxy cellulose and 15g of modified chitosan (the deacetylation degree of the chitin is 80%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 10%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:3 to ensure that the mass percentage content of tween 80 and span80 is 10 percent, and uniformly mixing;
(4) dropwise adding 170g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 6g of carbodiimide as a cross-linking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing 9% polyvinylpyrrolidone aqueous solution, adding glycerol according to the mass ratio of 1:10 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Comparative example 1
(1) Rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 50mg of nano silver prepared in the step (1), 10g of sodium hydroxy cellulose and 20g of modified chitosan (the deacetylation degree of chitin is 80%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 5%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:3 to ensure that the mass percentage of tween 80 and span80 is 8 percent, and uniformly mixing;
(4) dropwise adding 150g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 5g of carbodiimide as a cross-linking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 5%, adding glycerol according to the mass ratio of 1:20 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Comparative example 2
(1) Rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 40g of bacterial cellulose prepared in the step (1), 50mg of nano-silver and 20g of modified chitosan (the deacetylation degree of the chitin is 80%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 5%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:3 to ensure that the mass percentage of tween 80 and span80 is 8 percent, and uniformly mixing;
(4) dropwise adding 150g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 5g of carbodiimide as a cross-linking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 5%, adding glycerol according to the mass ratio of 1:20 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Comparative example 3
(1) Rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 40g of bacterial cellulose prepared in the step (1), 50mg of nano-silver and 10g of sodium hydroxy cellulose into an organosilicon quaternary ammonium salt solution, wherein the mass concentration of the organosilicon quaternary ammonium salt is 5%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:3 to ensure that the mass percentage of tween 80 and span80 is 8 percent, and uniformly mixing;
(4) dropwise adding 150g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 5g of carbodiimide as a cross-linking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 5%, adding glycerol according to the mass ratio of 1:20 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Comparative example 4
(1) Rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 40g of bacterial cellulose prepared in the step (1), 50mg of nano-silver, 10g of sodium hydroxy cellulose and 20g of modified chitosan (the deacetylation degree of the chitin is 80%) into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 5%, and uniformly mixing to obtain a mixed solution;
(3) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 5%, adding glycerol according to the mass ratio of 1:20 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding the mixed solution obtained in the step (2) according to the volume ratio of 1:20 of the mixed solution obtained in the step (2) to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Comparative example 5
(1) Rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 40g of bacterial cellulose prepared in the step (1), 50mg of nano-silver, 10g of sodium hydroxy cellulose, 20g of modified chitosan (the deacetylation degree of chitin is 80%), 120g of liquid paraffin, 10g of tween 80 and span80 (tween 80: span80 is 1:3) into an organosilicon quaternary ammonium salt solution, wherein the mass concentration of the organosilicon quaternary ammonium salt is 5%, and uniformly mixing to obtain a mixed emulsion;
(3) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 5%, adding glycerol according to the mass ratio of 1:20 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding the glycerol into the mixed emulsion according to the volume ratio of 1:20 of the mixed emulsion to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form the hydrogel.
Example 6 Release Rate testing
The test method comprises the following steps: citric acid was added to the hydrogels prepared in examples 1 to 5 and comparative examples 1 to 5, respectively, to adjust the pH to 5.5, the hydrogels were heated to 35 to 40 ℃ for accelerated experiments, and the total mass content of nano silver released from the hydrogels was measured every 0.5 hour. The nano silver is measured by adopting Raman spectrum, and the specific test conditions are as follows:
wavelength of excitation light source: 785nm
Excitation light source intensity: about 3mW
Magnification of objective lens: 10
Raman spectrum test interval: 300cm-1-900cm-1
Time interval of each raman spectrum test: 120 s.
The results are as follows:
table 1 release rate test results
The results show that examples 1 to 5 of the present invention have a better controlled release effect, and can prolong the release time, so that the release is more uniform, and a better bacteriostatic effect is achieved, and the hydrogel release rate of comparative examples 1 to 5 is too fast, and the dressing needs to be frequently replaced.
Claims (1)
1. A long-acting liquid dressing is characterized in that the preparation method of the long-acting liquid dressing is as follows:
(1) rinsing the wet flocculent bacterial cellulose, adding the rinsed wet flocculent bacterial cellulose into a NaOH solution with the mass concentration of 30%, heating, neutralizing by utilizing a hydrochloric acid solution with the mass concentration of 20%, and washing the mixture to be neutral by using deionized water;
(2) adding 40g of bacterial cellulose prepared in the step (1), 50mg of nano-silver, 10g of sodium hydroxy cellulose and 20g of modified chitosan with chitin deacetylation degree of 80% into an organic silicon quaternary ammonium salt solution, wherein the mass concentration of the organic silicon quaternary ammonium salt is 5%, and uniformly mixing to obtain a mixed solution;
(3) taking liquid paraffin, and mixing the liquid paraffin and the tween 80: adding span80 into liquid paraffin according to the mass ratio of 1:3 to ensure that the mass percentage of tween 80 and span80 is 8 percent, and uniformly mixing;
(4) dropwise adding 150g of the mixture prepared in the step (3) into the mixed solution prepared in the step (2), continuously stirring to form an emulsion in the dropwise adding process, wherein the stirring speed is 1200r/min, after dropwise adding is finished, adding 5g of carbodiimide as a cross-linking agent into the emulsion, continuously stirring, wherein the stirring speed is 1500r/min, centrifuging to remove an upper oil layer, retaining lower-layer particles, and washing, freeze-drying to obtain microcapsules;
(5) preparing a polyvinylpyrrolidone aqueous solution with the mass concentration of 5%, adding glycerol according to the mass ratio of 1:20 of the glycerol to the polyvinylpyrrolidone aqueous solution, adding microcapsules according to the volume ratio of 1:20 of the microcapsules to the polyvinylpyrrolidone aqueous solution, uniformly mixing, and performing irradiation treatment to form hydrogel, thus obtaining the long-acting liquid dressing.
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