CN109554300A - Utilize the method for oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems - Google Patents
Utilize the method for oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems Download PDFInfo
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- CN109554300A CN109554300A CN201910063084.6A CN201910063084A CN109554300A CN 109554300 A CN109554300 A CN 109554300A CN 201910063084 A CN201910063084 A CN 201910063084A CN 109554300 A CN109554300 A CN 109554300A
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- microalgae
- autoflocculation
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- algae
- municipal sewage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The present invention relates to a kind of methods using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems, including step A: adding step, certain density autoflocculation algae solution is put into oil-producing microalgae algae solution；Step B: flocculation step carries out flocculation treatment to the mixing algae solution that step A is obtained；Step C: separating step carries out algae water separating treatment.Oil-producing microalgae is harvested using the autoflocculation characteristic of autoflocculation microalgae, avoid the physically or chemically high energy consumption of flocculant and the pollution problem to final product, the production and operating cost during harvest are significantly reduced, is a kind of microalgae harvesting method efficiently, economic, environmentally friendly.Microalgae carries out growth metabolism and oil and fat accumulation using nutriments such as carbon, nitrogen, phosphorus in municipal sewage, promote the degradation of organic pollutants, and reduce the carbon emission during municipal sewage treatment to a certain extent, realize conversion of the sewage from pollutant to micro algae growth raw material.
Description
Technical field
The present invention relates to a kind of harvesting methods of microalgae more particularly to a kind of utilization autoflocculation microalgae harvesting municipal sewage to train
The method of oil-producing microalgae in the system of supporting.
Background technique
In face of the double challenge of energy crisis and environment water pollution, biology is produced using the oil-producing microalgae of municipal sewage culture
Diesel oil not only facilitates the new way for exploring a municipal sewage treatment, but also can alleviate the relatively deficient pressure of the energy.
Oil-producing microalgae production this technique of biodiesel using municipal sewage culture mainly includes the culture of microalgae, receives
It obtains, propose rouge and the links such as transesterification, wherein the expense of harvesting biomass accounts for the 20~30% of total cost of production, adds microalgae
Cell has the characteristics that small in size, density is low, is uniformly distributed in culture systems, so that efficient, the economic harvest of microalgae is still deposited
In technical bottleneck.The method of microalgae harvesting mainly includes centrifugation, air bearing, sedimentation, filtering, flocculation etc..Centrifugal process energy consumption is high；Filtering
Method is at high cost, and film is easily caused to block；Time-consuming for sedimentation；Gas floatation process needs to pre-process, and is not suitable for large-scale industrial application.
Currently, multi-pass crosses the efficient harvesting microalgae of flocculence, flocculation can be divided into chemistry, physics and bioflocculation, but chemistry
The use of flocculant will increase production cost, and residue can also bring puzzlement to downstream processing；And the at high cost, energy of physics flocculation
Consumption is big；Bioflocculation can pollution to avoid chemical floc to final product, but technical requirements or higher cost.
For example, CN101748068A discloses a kind of microalgae harvesting method, mainly there are High Density Cultivation Photoreactor, sedimentation
Pond, immersible pump, day photosystem, five part of pulverizer/grinder composition.It the steps include: A, sedimentation；B, supernatant is removed, immersible pump is used
Upper layer algae solution is taken away, is recycled；C, drying, lower layer are concentrated algae solution solar radiation, dry, and form algae piece；D, using
Algae piece algae powder can be used to following process by pulverizer or grinding.The method operation is relatively simple, but often due to frond
It is not easy to settle, so harvest process takes a long time, it is difficult to meet the harvest rate requirement of large-scale production.
CN 106995248A discloses one kind, and the invention discloses a kind of green safe, quick microalgae harvesting method, head
First with food-grade natural macromolecular flocculant " charge neutrality " algae particle surface negative electrical charge, promote algae particle loss of stability, and
Algae wadding body is further formed under " bridging and netting " effect of macromolecular chain.Then it is adhered to using the micro-bubble that electric floating generates
Algae wadding body, forms water-gas-wadding body three-phase mixed system, and floating upward quickly to the water surface realizes that algae is quickly collected.The method is time-consuming
It is shorter, but need that high-power power is maintained to export, consume energy higher, and food-grade natural macromolecular flocculant often cost compared with
It is high, it is difficult to meet algae bio fuel industry metaplasia and produce to inexpensive requirement.
Therefore, a kind of become in the research of algae bio fuel with cost-benefit green harvesting method is found most to challenge
Property one of field, and the limitation commercialized key factor of microalgae.
Summary of the invention
In view of technical problem of the existing technology, the present invention constructs a kind of utilization autoflocculation microalgae harvesting municipal sewage training
The method of oil-producing microalgae in the system of supporting, for overcoming, existing microalgae harvesting method is at high cost, energy consumption is high, is difficult to industrial applications
Problem.
The technical scheme is that providing a kind of micro- using oil-producing in autoflocculation microalgae harvesting municipal sewage culture systems
The method of algae, includes the following steps:
Step A: step is added, the step system that adds is by certain density autoflocculation algae solution investment oil-producing microalgae algae solution
In；
Step B: flocculation step, the flocculation step system carry out flocculation treatment to the mixing algae solution that step A is obtained；
Step C: separating step, the floccule body that the separating step system takes step B to obtain carry out algae water separating treatment.
Since formed wadding body volume is larger, using filter cloth, the separation of algae water is realized under the effect of gravity.Pass through this method
Green, efficient, economical oil-producing microalgae can be harvested.
A further technical solution of the present invention is: the oil-producing microalgae is mutagenesis, domestication, screening gained chlorella, tiltedly life
One or more of scenedesmus, Closterium, Dunaliella salina algae, 20% or more fat content.This method is applied widely.
A further technical solution of the present invention is: the autoflocculation microalgae is the filiform for being easy to settle of municipal sewage culture
One or more of algae, empty star algae, crescent algae fibre, scenedesmus.Sedimentation rate in 12h is up to 90% or more.
A further technical solution of the present invention is: passing through measurement OD680, autoflocculation microalgae and oil-producing microalgae are in city dirt
Culture is harvested when in highest biomass to early stage stationary phase in water.
A further technical solution of the present invention is: autoflocculation algae solution is uniform outstanding at distribution using dispersal mechanism in step A
Liquid, the revolving speed of dispersion machine are 1000-1500rpm；When autoflocculation algae dosage is less, the oil-producing microalgae for capableing of adsorption-flocculation is thin
Born of the same parents are limited, when autoflocculation algae dosage is excessive, the mutual absorption between autoflocculation microalgae easily formed, to influence to oil-producing microalgae
Absorption, can reduce the yield rate to oil-producing microalgae.Therefore autoflocculation algae dosage exist more excellent concentration, experiment gained compared with
Excellent concentration ranges are 0.06~0.10g/L.
A further technical solution of the present invention is: the pH of algae solution is mixed by regulation in step B, adds calcium and magnesium ion etc.
Condition induces the formation of algae floccule body, and above-mentioned condition plays decisive role to the efficiency of crop of microalgae.
A further technical solution of the present invention is: first when utilizing autoflocculation microalgae harvesting oil-producing microalgae in the step B
Beginning, pH was set as 8~10.
A further technical solution of the present invention is: the concentration for adding calcium and magnesium ion in the step B is 4~6mmoL/L.
A further technical solution of the present invention is: mixing algae solution using concussion shaking table in the step B and accelerating algae flocculation
The formation of body, the revolving speed of shaking table are 120~140rpm.
A further technical solution of the present invention is: forming microalgae to be sufficiently mixed autoflocculation algae and oil-producing microalgae
Wadding body after step B carries out 6~12h, stands 30min and carries out step C again.
A further technical solution of the present invention is: filter cloth aperture is too small, filtering rate is influenced；Aperture is excessive, will cause algae
Body is lost.The separation of algae water is carried out using the filter cloth of 60~100 mesh in step C.Algae solution passes through filter cloth, microalgae wadding under the effect of gravity
Body is trapped on filter cloth, realizes the separation of algae water.It must be filtered by vacuum using filter membrane when individually being harvested compared to oil-producing microalgae, it can be big
Width reduces algae water separating energy consumption.
The beneficial effects of the present invention are:
This programme constructs a kind of method using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems, from
Flocculation microalgae has advantage safe and environment-friendly, at low cost, easy to operate；Meanwhile organic matter, nitrogen phosphorus in city domestic sewage
Equal nutrition contents height is conducive to microalgae mass propagation, realizes conversion of the sewage from pollutant to micro algae growth raw material, is
Microalgae harvesting provides a kind of technology with good industrial prospect.
Oil-producing microalgae is harvested using the autoflocculation characteristic of autoflocculation microalgae, avoids the high energy consumption of physically or chemically flocculant
And the pollution problem to final product, the production and operating cost during harvest are significantly reduced, is a kind of efficient, economy, ring
The microalgae harvesting method of guarantor.Microalgae carries out growth metabolism and grease product using nutriments such as carbon, nitrogen, phosphorus in municipal sewage
It is tired, the degradation of organic pollutants is promoted, and reduce the carbon emission during municipal sewage treatment to a certain extent,
Realize conversion of the sewage from pollutant to micro algae growth raw material.
Detailed description of the invention
Fig. 1 is flow chart of the invention, wherein 1, autoflocculation microalgae suspension；2, oil-producing microalgae；3, algae solution is mixed；4, it shakes
Bed；5, floccule body.
Specific embodiment
Combined with specific embodiments below and attached drawing, more excellent case study on implementation of the invention is described in further detail, but
The present invention is not limited to this.
The present invention provides a kind of method using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems.Below
In conjunction with accompanying drawings and embodiments, the present invention is described in detail.
As shown in Figure 1, a specific embodiment of the invention is: providing a kind of utilization autoflocculation microalgae harvesting municipal sewage training
The method of oil-producing microalgae in the system of supporting, comprising: 1, autoflocculation microalgae suspension；2, oil-producing microalgae；3, algae solution is mixed；4, shaking table；5,
Floccule body.
Specific implementation process is as follows: finely dispersed autoflocculation microalgae suspension 1 is made in certain density autoflocculation microalgae
It puts into the oil-producing microalgae algae solution 2 of municipal sewage culture, after calcium and magnesium ion is added into mixing algae solution 3 and adjusts pH, is put
It is placed on shaking table 4 and carries out flocculation reaction, after the completion of flocculation, separated floccule body 5 and municipal sewage using filter cloth.Specific embodiment
In, the autoflocculation microalgae be the filamentous algae for being easy to settle of municipal sewage culture, empty star algae, crescent algae fibre, in scenedesmus
It is one or more of.In specific embodiment, the oil-producing microalgae is mutagenesis, domestication, screening gained chlorella, scenedesmus obliquus, crescent
One or more of algae, Dunaliella salina algae.
This method is applied widely.In specific embodiment, autoflocculation algae solution is uniform outstanding at distribution using dispersal mechanism
Liquid, the revolving speed of dispersion machine are 1000~1500rpm, 0.06~0.10g/L of dosage.In specific embodiment, mixed by regulation
The pH of algae solution, the formation for adding the conditions such as calcium and magnesium ion induction microalgae floccule body；The initial pH for mixing algae solution is 8~10；It adds
The concentration of calcium and magnesium ion is 4~6mmoL/L.In specific embodiment, algae solution is mixed using concussion shaking table and accelerates algae floccule body
It is formed, the revolving speed of shaking table is 120~140rpm.In specific embodiment, after flocculation process carries out 6~12h, stands 30min and adopt again
Algae water separation is carried out with the filter cloth of 60~100 mesh.
Embodiment 1
Using municipal sewage by autoflocculation filamentous algae culture to stationary phase (dry weight about 1g/L or so), existed with dispersion machine
More uniform suspension is formed under 1000rpm revolving speed.The scenedesmus obliquus algae solution of 90mL municipal sewage culture is placed in 150mL taper
In bottle, the autoflocculation filamentous algae suspension 10ml mixed is added.The initial pH to 9 of mixing algae solution is adjusted, calcium ion concentration is
5mmoL/L is placed in the revolving speed immixture 12h on shaking table with 130rpm.After standing 30min, carried out using the filter cloth of 80 mesh
The separation of algae water, the yield rate of gained oil-producing scenedesmus obliquus is up to 84%.
Embodiment 2
By autoflocculation scenedesmus culture to stationary phase (dry weight about 1g/L or so), formed under 1500rpm revolving speed with dispersion machine
More uniform suspension.The mixing algae solution of the chlorella of 90mL municipal sewage culture and scenedesmus obliquus is placed in 150mL conical flask
In, the autoflocculation scenedesmus suspension 10ml mixed is added.Adjust the initial pH to 9, calcium ion concentration 5mmoL/ of mixing algae solution
L is placed in the revolving speed immixture 10h on shaking table with 120rpm.After standing 30min, gained oil-producing chlorella and scenedesmus obliquus
The yield rate of mixed liquor is up to 86%.
Comparative example 1
Design sedimentation tank depth is 10cm, and according to barodynamics principle, municipal sewage culture is reached to the oil-containing of growth stationary phase
Chlorella stands naturally in settling tank, and after 24 hours, microalgae cell sedimentation rate is about 60%, and since frond is smaller, with pump
When supernatant liquor is detached, frond disturbance is easily caused to be resuspended, and microalgae harvesting rate is lower.
Comparative example 2
By autoflocculation scenedesmus culture to stationary phase (dry weight about 1g/L or so), formed under 1500rpm revolving speed with dispersion machine
More uniform suspension.The mixing algae solution of the chlorella of 98mL municipal sewage culture and scenedesmus obliquus is placed in 150mL conical flask
In, the autoflocculation scenedesmus suspension 2ml mixed is added.Initial pH to 9, the calcium ion concentration 1mmoL/L of mixing algae solution are adjusted,
It is placed in the revolving speed immixture 10h on shaking table with 120rpm.After standing 30min, gained oil-producing chlorella and scenedesmus obliquus are mixed
The yield rate for closing liquid is 43%.
From the above and comparing result is as it can be seen that this programme constructs a kind of utilization autoflocculation microalgae harvesting municipal sewage training
The method of oil-producing microalgae in the system of supporting more is pacified using autoflocculation microalgae harvesting oil-producing microalgae compared to traditional chemical method harvest
Entirely, environmentally friendly, there is advantage at low cost, easy to operate compared to physics harvest；Meanwhile organic matter in city domestic sewage,
The nutrition contents height such as nitrogen phosphorus is conducive to microalgae mass propagation, realizes sewage and turns from pollutant to micro algae growth raw material
Change, provides a kind of technology with good industrial prospect for microalgae harvesting.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
Specific implementation of the invention is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to of the invention
Protection scope.
Claims (10)
1. a kind of method using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems, which is characterized in that including
Following steps:
Step A: adding step, and the step system that adds puts into certain density autoflocculation algae solution in oil-producing microalgae algae solution；
Step B: flocculation step, the flocculation step system carry out flocculation treatment to the mixing algae solution that step A is obtained；
Step C: separating step, the floccule body that the separating step system takes step B to obtain carry out algae water separating treatment.
2. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 1
Method, which is characterized in that the oil-producing microalgae is mutagenesis, domestication, screening gained chlorella, scenedesmus obliquus, Closterium, Dunaliella salina
One or more of algae；The autoflocculation microalgae is the filamentous algae for being easy to settle of municipal sewage culture, empty star algae, crescent
One or more of algae fibre, scenedesmus.
3. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 1
Method, which is characterized in that autoflocculation microalgae and oil-producing microalgae are in early stage stationary phase.
4. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 1
Method, which is characterized in that autoflocculation algae solution is at uniform suspension, the revolving speed of dispersion machine is distributed using dispersal mechanism in step A
1000-1500rpm；0.06~0.10g/L of dosage of autoflocculation microalgae.
5. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 1
Method, which is characterized in that the pH of algae solution is mixed by regulation in step B, adds the conditions such as calcium and magnesium ion induction microalgae floccule body
It is formed.
6. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 5
Method, which is characterized in that initial pH when in the step B using autoflocculation microalgae harvesting oil-producing microalgae is set as 8~10.
7. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 5
Method, which is characterized in that the concentration that calcium and magnesium ion is added in the step B is respectively 4~6mmoL/L.
8. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 1
Method, which is characterized in that mix algae solution using concussion shaking table in the step B and accelerate the formation of algae floccule body, the revolving speed of shaking table
For 120~140rpm.
9. a kind of side using oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems according to claim 1
Method, which is characterized in that after step B carries out 6~12h, stand 30min and carry out step C again.
10. a kind of oil-producing microalgae in autoflocculation microalgae harvesting municipal sewage culture systems is utilized according to claim 1
Method, which is characterized in that the separation of algae water is carried out using the filter cloth of 60~100 mesh in step C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110387332A (en) * | 2019-07-09 | 2019-10-29 | 天津大学 | It is a kind of to be accumulated using artificial municipal wastewater culture chlorella and extract the research method of protein |
| CN111908679A (en) * | 2020-07-16 | 2020-11-10 | 重庆工商大学 | Method for removing algae pollutants in water body by improving algae self-flocculation capacity |
| CN112322499A (en) * | 2020-10-29 | 2021-02-05 | 深圳市鑫承诺环保产业股份有限公司 | Autotrophic heterotrophic alternative domestication cultured dunaliella salina and application thereof in treatment of marine ship domestic sewage |
| CN114133039A (en) * | 2021-12-01 | 2022-03-04 | 北京林业大学 | Method for treating urban domestic sewage by using mixed microalgae |
-
2019
- 2019-01-23 CN CN201910063084.6A patent/CN109554300B/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110387332A (en) * | 2019-07-09 | 2019-10-29 | 天津大学 | It is a kind of to be accumulated using artificial municipal wastewater culture chlorella and extract the research method of protein |
| CN111908679A (en) * | 2020-07-16 | 2020-11-10 | 重庆工商大学 | Method for removing algae pollutants in water body by improving algae self-flocculation capacity |
| CN112322499A (en) * | 2020-10-29 | 2021-02-05 | 深圳市鑫承诺环保产业股份有限公司 | Autotrophic heterotrophic alternative domestication cultured dunaliella salina and application thereof in treatment of marine ship domestic sewage |
| CN114133039A (en) * | 2021-12-01 | 2022-03-04 | 北京林业大学 | Method for treating urban domestic sewage by using mixed microalgae |
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