CN109527193A - A kind of preparation method for the gadus collagen peptide that phosphorylation is modified - Google Patents
A kind of preparation method for the gadus collagen peptide that phosphorylation is modified Download PDFInfo
- Publication number
- CN109527193A CN109527193A CN201811652077.1A CN201811652077A CN109527193A CN 109527193 A CN109527193 A CN 109527193A CN 201811652077 A CN201811652077 A CN 201811652077A CN 109527193 A CN109527193 A CN 109527193A
- Authority
- CN
- China
- Prior art keywords
- gadus
- collagen peptide
- phosphorylation
- modified
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a kind of preparation methods of the modified gadus collagen peptide of phosphorylation comprising following steps: (1) successively in clear water, sodium bicarbonate solution, citric acid solution, EDTA solution cleans gadus scale；(2) protease is added in the gadus scale after being cleaned in step 1, hydrolyzes then inactivation, centrifugation obtains supernatant；(3) the supernatant desalination for obtaining step 2, separation discard 1000Da component below, are lyophilized, obtain gadus collagen peptide dry powder, and be dissolved into water；(4) solution of step 3 is heated, adjusts pH, sodium tripolyphosphate is then added, is stirred to react；(5) reaction solution of collection step 4, desalination, freeze-drying obtain the modified gadus collagen peptide of phosphorylation.Method of the invention realizes the more efficient progress of phosphorylation processing, has broad application prospects by pre-processing to collagen.
Description
Technical field
The present invention relates to the preparation of peptide, the preparation method of the modified gadus collagen peptide of especially a kind of phosphorylation.
Background technique
Gadus is the general designation of other section part fish of gadidae (Gadidae) fish and Gadiformes (Gadiformes), there is 50
It is a variety of, it is one of main edible fishes.Gadus originates in from Northern Europe to Canada and North Atlantic Ocean cold waters of eastern United States.
At the beginning of 21 century, the main country of origin of gadus is Canada, Iceland, Norway and Russia, and the Japanese place of production is mainly in Hokkaido.Gadus is
Its flesh of fish is often used for the purposes such as fried by the higher high-level fish of economic value.However its fish scale and fish-skin often cannot get it is good
Good utilization often can only be processed into fertilizer or feed as leftover bits and pieces, and economic value added is lower.Currently, can be by it
Fish scale and fish-skin are prepared into collagen peptide, however the dispersibility of the collagen peptide in water is poor, it is difficult to as top grade
The purposes such as oral solution come carry out using.
Summary of the invention
In order to overcome the shortcomings of the bad dispersibility of existing gadus collagen peptide in water, the purpose of the present invention is to provide
A kind of preparation method for the gadus collagen peptide that phosphorylation is modified.
In one aspect of the invention, a kind of preparation method of gadus collagen peptide that phosphorylation is modified is provided,
Be characterized in that, the method the following steps are included:
(1) gadus scale is successively molten in clear water, the sodium bicarbonate solution of 1-5%, the citric acid solution of 1-3%, the EDTA of 1-5%
It is cleaned in liquid；
(2) protease will be added in the gadus scale after cleaning obtained in step 1, and under 40-50 degrees Celsius, pH 6-
It is hydrolyzed 3-5 hours under 8 and then inactivates, be centrifuged and obtain supernatant；
(3) the supernatant desalination for obtaining step 2, separation discard 1000Da component below, are lyophilized and obtain gadus collagen
Protein peptides dry powder takes 100 parts by weight, and is dissolved into the water of 10000-20000 parts by weight；
(4) solution of step 3 is heated to 30-40 degrees Celsius, pH is adjusted to 7.5-8.5, and sodium tripolyphosphate 1-5 weight is then added
Part is measured, and is stirred to react 2-5 hours；
(5) reaction solution of collection step 4, desalination and freeze-drying obtain the modified gadus collagen peptide of phosphorylation.
In currently preferred aspect, in step 1, by gadus scale successively clear water, 2% sodium bicarbonate solution, 2%
Citric acid solution, 3% EDTA solution in clean, every time cleaning 15 minutes.
In currently preferred aspect, in step 2, under 42 degrees Celsius, pH be 7.2 lower hydrolysis 4.5 hours and with
After inactivate, be centrifuged and obtain supernatant.
In currently preferred aspect, in step 2, the protease be neutral proteinase and alkali protease according to
1:1-3 weight ratio is configured to.
In currently preferred aspect, in step 2, the protease is neutral proteinase or alkali protease.
In currently preferred aspect, in step 3, the separation takes gel exclusion chromatography or dialysis process
It carries out.
In currently preferred aspect, the solution of step 3 is heated to 35 degrees Celsius, pH is adjusted to 8.0, and trimerization phosphorus is added
Sour 3.5 parts by weight of sodium, and be stirred to react 4 hours.
Method of the invention realizes the more efficient progress of phosphorylation processing, tool by pre-processing to collagen
Have broad application prospects.
Specific embodiment
Unless additionally illustrating, gadus scale used in the present invention purchases the leftover bits and pieces area from local aquatic products processing factory
Domain saves under 0-4 degrees Celsius always and keeps fresh.
Embodiment 1
In the present embodiment, a kind of preparation method of the modified gadus collagen peptide of phosphorylation comprising following steps:
(1) by 10 kilograms of gadus scales successively clear water, 2% sodium bicarbonate solution, 2% citric acid solution, 3% EDTA it is molten
It is cleaned in liquid, every time cleaning 15 minutes；
(2) protease will be added in the gadus scale after cleaning obtained in step 1, also, under 42 degrees Celsius, pH 7.2
It is lower to hydrolyze 4.5 hours and then inactivate, it is centrifuged and obtains supernatant, the protease is neutral proteinase and alkaline egg
White enzyme is configured to according to 1:1-3 weight ratio；
(3) the supernatant desalination for obtaining step 2, and deionized water is permeated 10 hours using in the semi-permeable membrane of 1000Da,
It is lyophilized and is obtained 544 grams of gadus collagen peptide dry powder, takes 100 grams, and is dissolved into 15 liters of water；
(4) solution of step 3 is heated to 35 degrees Celsius, pH is adjusted to 8.0, is then added 3.5 grams of sodium tripolyphosphate, and stir
Mix reaction 4 hours；
(5) reaction solution of collection step 4, desalination and freeze-drying obtain the modified gadus collagen peptide of phosphorylation.
Embodiment 2
In the present embodiment, a kind of preparation method of the modified gadus collagen peptide of phosphorylation comprising following steps:
(1) by 10 kilograms of gadus scales successively clear water, 5% sodium bicarbonate solution, 3% citric acid solution, 1% EDTA it is molten
It is cleaned in liquid, every time cleaning 15 minutes；
(2) protease is added in the gadus scale after cleaning obtained in step 1, and at 50 c, pH is lauched for 8
It solves 3 hours and then inactivates, be centrifuged and obtain supernatant, the protease is alkali protease；
(3) the supernatant desalination for obtaining step 2, and deionized water is permeated 12 hours using in the semi-permeable membrane of 1000Da,
It is lyophilized and is obtained 459 grams of gadus collagen peptide dry powder, takes 100 grams, and is dissolved into 20 liters of water；
(4) solution of step 3 is heated to 40 degrees Celsius, pH is adjusted to 8.5, is then added 5 grams of sodium tripolyphosphate, and stir
Reaction 2 hours；
(5) reaction solution of collection step 4, desalination and freeze-drying obtain the modified gadus collagen peptide of phosphorylation.
Embodiment 3
In the present embodiment, a kind of preparation method of the modified gadus collagen peptide of phosphorylation comprising following steps:
(1) by 10 kilograms of gadus scales successively clear water, 1% sodium bicarbonate solution, 1% citric acid solution, 5% EDTA it is molten
It is cleaned in liquid, every time cleaning 15 minutes；
(2) protease is added in the gadus scale after cleaning obtained in step 1, and under 40 degrees Celsius, pH is lauched for 6
It solves 5 hours and then inactivates, be centrifuged and obtain supernatant, the protease is neutral proteinase；
(3) the supernatant desalination for obtaining step 2, and 1000Da ingredient below is removed using gel exclusion chromatography method,
It is lyophilized and is obtained 551 grams of gadus collagen peptide dry powder, takes 100 grams, and is dissolved into 10 liters of water；
(4) solution of step 3 is heated to 30 degrees Celsius, pH is adjusted to 7.5, is then added 1 gram of sodium tripolyphosphate, and stir
Reaction 5 hours；
(5) reaction solution of collection step 4, desalination and freeze-drying obtain the modified gadus collagen peptide of phosphorylation.
Comparative example 1
It is in this comparative example with the difference of embodiment 1, collagen peptide is not separated in step 3.Obtain gadus
605 grams of collagen peptide dry powder, and take 100 grams of tests carried out hereafter therein.
Comparative example 2
It is in this comparative example with the difference of embodiment 1, eliminates 100Da using gel exclusion chromatography method in step 3
Ingredient below.588 grams of gadus collagen peptide dry powder is obtained, and takes 100 grams of tests carried out hereafter therein.
Comparative example 3
This comparative example and embodiment 1 the difference is that:
(1) collagen peptide is not separated in step 3；
(2) in step 4,7 grams of sodium tripolyphosphate are added；
621 grams of gadus collagen peptide dry powder is obtained, and takes 100 grams of tests carried out hereafter therein.
Comparative example 4
This comparative example and embodiment 1 the difference is that:
(1) collagen peptide is not separated in step 3；
(2) in step 4,15 grams of sodium tripolyphosphate are added.
597 grams of gadus collagen peptide dry powder is obtained, and takes 100 grams of tests carried out hereafter therein.
One, stability test
In this test, stabilization of collagen peptide during deploying oral solution in each embodiment and comparative example is determined
Property.The not phosphorylated modification of the phosphorylation modified collagen protein peptides and embodiment 1 of Example 1-3 and comparative example 1-2
Collagen peptide, the allotment for carrying out oral solution according to the charge ratio of table 1.
Table 1: the formula table of each test group oral solution
| Test group | Collagen peptide selection | Collagen peptide dosage/g | Hyaluronic acid/g | Water | Additional conditions |
| A01 | After embodiment 1- phosphorylation | 7.0g | 1.0g | 93.0mL | Nothing |
| B01 | After embodiment 2- phosphorylation | 7.0g | 1.0g | 93.0mL | Nothing |
| C01 | After embodiment 3- phosphorylation | 7.0g | 1.0g | 93.0mL | Nothing |
| D01 | After comparative example 1- phosphorylation | 7.0g | 1.0g | 93.0mL | Nothing |
| E01 | After comparative example 2- phosphorylation | 7.0g | 1.0g | 93.0mL | Nothing |
| F01 | Before embodiment 1- phosphorylation | 7.0g | 1.0g | 93.0mL | Nothing |
Test group A01-E01 obtains clarifying thorough oral solution after 2 hours of stirring, and F01 still has certain flocks shape
Solid and stirring 12 hours after be completely dissolved.One skilled in the art will appreciate that general collagen peptide is daily oral
Amount is 7 grams or so, and the consumer group is based on women, and therefore, it is desirable to taking the photograph for collagen peptide is completed in volume as few as possible
Enter.In general, collagen peptide is given with the bottle containing hyaluronic acid of 100mL or so.It can be seen that each
Embodiment and comparative example, and the collagen peptide without phosphatizing treatment may be configured to as the good drink of appearance.
In view of Ms takes the demand for taste, the drink sample test at different pH has also been attempted.Above
A-F on the basis of, to the collagen peptide solution of each group respectively take its half, carried out filling CO 2 operation.The formula of each group
It is recorded in table 2.
Table 2: the formula of each test group inflation oral solution
| Test group | Collagen peptide selection | Collagen peptide dosage/g | Hyaluronic acid/g | Water | Additional conditions |
| A02 | After embodiment 1- phosphorylation | 3.5g | 0.5g | 46.5mL | Carbon dioxide saturation inflation |
| B02 | After embodiment 2- phosphorylation | 3.5g | 0.5g | 46.5mL | Carbon dioxide saturation inflation |
| C02 | After embodiment 3- phosphorylation | 3.5g | 0.5g | 46.5mL | Carbon dioxide saturation inflation |
| D02 | After comparative example 1- phosphorylation | 3.5g | 0.5g | 46.5mL | Carbon dioxide saturation inflation |
| E02 | After comparative example 2- phosphorylation | 3.5g | 0.5g | 46.5mL | Carbon dioxide saturation inflation |
| F02 | Before embodiment 1- phosphorylation | 3.5g | 0.5g | 46.5mL | Carbon dioxide saturation inflation |
In the case where carbon dioxide is saturated and inflates, discovery A02, B02, C02, D02, E02 keep clarifying, but F02 starts
There is flocculent deposit, slight flocculent deposit occurs in D02.This shows without phosphatizing treatment, in separate equal electricity
The solubility of (pH is 5.6 or so at this time) collagen peptide is decreased obviously when point.
In addition to this, also being adjusted the remaining half of the oral solution of each group to pH using citric acid is 6.0, for simulating acid
The formula of flavor beverage, and the state of each group collagen peptide solution is observed, the formula of each group is referring to table 3:
Table 3: the formula of each test group acidification oral solution
| Test group | Collagen peptide selection | Collagen peptide dosage/g | Hyaluronic acid/g | Water | Additional conditions |
| A03 | After embodiment 1- phosphorylation | 3.5g | 0.5g | 46.5mL | Lemon acid for adjusting pH is to 6.0 |
| B03 | After embodiment 2- phosphorylation | 3.5g | 0.5g | 46.5mL | Lemon acid for adjusting pH is to 6.0 |
| C03 | After embodiment 3- phosphorylation | 3.5g | 0.5g | 46.5mL | Lemon acid for adjusting pH is to 6.0 |
| D03 | After comparative example 1- phosphorylation | 3.5g | 0.5g | 46.5mL | Lemon acid for adjusting pH is to 6.0 |
| E03 | After comparative example 2- phosphorylation | 3.5g | 0.5g | 46.5mL | Lemon acid for adjusting pH is to 6.0 |
| F03 | Before embodiment 1- phosphorylation | 3.5g | 0.5g | 46.5mL | Lemon acid for adjusting pH is to 6.0 |
Observing that obvious flocculent deposit occurs in the oral solution of F03, there are slight flocks in D03, though the solution of C03 and E03
Do not occur flocks so, but becomes slight translucent milky white shape.Remaining each group still maintains clarification.
It is above experiments have shown that, although the dissolubility of collagen oral liquid is generally preferable, it is desirable that preparing has
When the oral solution of particular tastes, certain flocks risk is just faced.The flocks wind is helped avoid using phosphatizing treatment mode
The generation of danger.And in the case where being also surprising that under same phosphorylation level without low molecular weight removal, phosphorus
The anticoagulant wadding effect of acidification bring is just declined.
Comparative example 3-4 is then prepared into common drink, carbon dioxide saturation inflation drink and lemon according to above method
Lemon acid for adjusting pH is recorded as G01, G02, G03, H01, H02 and H03 to 6.0 drink respectively.Wherein G01 and H01 are limpid
Bright, slight translucent milky white shape is presented in G02 and G03, and H02 and H03 keeps limpid.This shows to separate without collagen
Under the premise of, it needs to greatly improve the dosage of sodium tripolyphosphate, the effect of abundant phosphorylation also may be implemented.
Two, solution rate is tested
In addition to this, also to the modified collagen peptide of the phosphorylation of each embodiment and comparative example, embodiment 1 without phosphorus
Acidified modified collagen peptide has carried out the test of solution rate.Specific test method is as follows:
8,500mL beaker are taken, and is fed intake according to the formula of the correspondence test group in stability test, with identical big
Small magneton is stirred under 200 turns of speed per minute.It is primary every observation in 15 minutes, and record various time points
The dissolution situation of collagen peptide.Wherein still there is fluffy solid to be recorded as " 2 ", translucent milky white shape is recorded as " 1 ", completely limpid
It is recorded as " 0 ".Test data records in table 4.After numerical value " 0 " is recorded, stop the observation to the sample.
Table 4: each test group sample solution rate record
The sample that can be seen that embodiment 1-3 from above solution rate can complete within an hour dissolution, tool in stirring
There is good solubility property.In the case where separating without collagen, comparative example 1 has poor solubility property.This is molten
Solution performance improves with the removing of collagen low molecular weight compositions, and good also with the dosage for improving phosphorylation agent
Turn.Product without phosphorylation is not completely dissolved always.
Claims (8)
1. a kind of preparation method of the modified gadus collagen peptide of phosphorylation, which is characterized in that the method includes following
Step:
(1) gadus scale is successively molten in clear water, the sodium bicarbonate solution of 1-5%, the citric acid solution of 1-3%, the EDTA of 1-5%
It is cleaned in liquid；
(2) protease will be added in the gadus scale after cleaning obtained in step 1, and under 40-50 degrees Celsius, pH 6-
It is hydrolyzed 3-5 hours under 8 and then inactivates, be centrifuged and obtain supernatant；
(3) the supernatant desalination for obtaining step 2, separation discard 1000Da component below, are lyophilized and obtain gadus collagen
Protein peptides dry powder takes 100 parts by weight, and is dissolved into the water of 10000-20000 parts by weight；
(4) solution of step 3 is heated to 30-40 degrees Celsius, pH is adjusted to 7.5-8.5, and sodium tripolyphosphate 1-5 weight is then added
Part is measured, and is stirred to react 2-5 hours；
(5) reaction solution of collection step 4, desalination and freeze-drying obtain the modified gadus collagen peptide of phosphorylation.
2. a kind of preparation method of the modified gadus collagen peptide of phosphorylation according to claim 1, which is characterized in that
In step 1, by gadus scale successively clear water, 2% sodium bicarbonate solution, 2% citric acid solution, 3% EDTA solution in
Cleaning is cleaned 15 minutes every time.
3. a kind of preparation method of the modified gadus collagen peptide of phosphorylation according to claim 1, which is characterized in that
In step 2, under 42 degrees Celsius, pH is 7.2 lower hydrolysis 4.5 hours and then inactivates, be centrifuged and obtain supernatant.
4. a kind of preparation method of the modified gadus collagen peptide of phosphorylation according to claim 1, which is characterized in that
In step 2, the protease is that neutral proteinase and alkali protease are configured to according to 1:1-3 weight ratio.
5. a kind of preparation method of the modified gadus collagen peptide of phosphorylation according to claim 1, which is characterized in that
In step 2, the protease is neutral proteinase or alkali protease.
6. a kind of preparation method of the modified gadus collagen peptide of phosphorylation according to claim 1, which is characterized in that
In step 3, the separation takes gel exclusion chromatography or dialysis process to carry out.
7. a kind of preparation method of the modified gadus collagen peptide of phosphorylation according to claim 1, which is characterized in that
In step 3, supernatant desalination step 2 obtained, and using small to deionized water infiltration 10 in the semi-permeable membrane of 1000Da
When, it is lyophilized and is obtained 544 grams of gadus collagen peptide dry powder, takes 100 grams, and is dissolved into 15 liters of water.
8. a kind of preparation method of the modified gadus collagen peptide of phosphorylation according to claim 1, which is characterized in that
The solution of step 3 is heated to 35 degrees Celsius, pH is adjusted to 8.0,3.5 parts by weight of sodium tripolyphosphate is added, and be stirred to react 4
Hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811652077.1A CN109527193A (en) | 2018-12-31 | 2018-12-31 | A kind of preparation method for the gadus collagen peptide that phosphorylation is modified |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811652077.1A CN109527193A (en) | 2018-12-31 | 2018-12-31 | A kind of preparation method for the gadus collagen peptide that phosphorylation is modified |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109527193A true CN109527193A (en) | 2019-03-29 |
Family
ID=65833807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811652077.1A Pending CN109527193A (en) | 2018-12-31 | 2018-12-31 | A kind of preparation method for the gadus collagen peptide that phosphorylation is modified |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109527193A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113957111A (en) * | 2021-10-15 | 2022-01-21 | 海南华研胶原科技股份有限公司 | Preparation method of phosphorylation modified cod collagen peptide |
| CN114560927A (en) * | 2022-03-15 | 2022-05-31 | 厦门元之道生物科技有限公司 | Method for producing collagen peptide by subcritical water extraction assisted fish scales and application of method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100999752A (en) * | 2006-01-13 | 2007-07-18 | 中国农业大学 | Antioxydizing peptide mixture from collagen and its preparation process and use |
| CN101418328A (en) * | 2008-10-28 | 2009-04-29 | 山东好当家海洋发展股份有限公司 | Method for producing fish scale collagen protein |
| CN103044541A (en) * | 2013-01-15 | 2013-04-17 | 青岛贝尔特生物科技有限公司 | Method for preparing phosphorylated collagen |
| CN104839760A (en) * | 2015-05-06 | 2015-08-19 | 河南科技大学 | Process method for modified collaborative preparation of egg white powder through enzymolysis and phosphorylation |
-
2018
- 2018-12-31 CN CN201811652077.1A patent/CN109527193A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100999752A (en) * | 2006-01-13 | 2007-07-18 | 中国农业大学 | Antioxydizing peptide mixture from collagen and its preparation process and use |
| CN101418328A (en) * | 2008-10-28 | 2009-04-29 | 山东好当家海洋发展股份有限公司 | Method for producing fish scale collagen protein |
| CN103044541A (en) * | 2013-01-15 | 2013-04-17 | 青岛贝尔特生物科技有限公司 | Method for preparing phosphorylated collagen |
| CN104839760A (en) * | 2015-05-06 | 2015-08-19 | 河南科技大学 | Process method for modified collaborative preparation of egg white powder through enzymolysis and phosphorylation |
Non-Patent Citations (1)
| Title |
|---|
| 唐旭，等: "碱性蛋白酶水解海洋鱼鳞工艺改进", 厦门大学学报（自然科学版）, vol. 51, no. 6, pages 1097 - 1102 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113957111A (en) * | 2021-10-15 | 2022-01-21 | 海南华研胶原科技股份有限公司 | Preparation method of phosphorylation modified cod collagen peptide |
| CN113957111B (en) * | 2021-10-15 | 2024-01-05 | 海南华研胶原科技股份有限公司 | Preparation method of phosphorylation modified cod collagen peptide |
| CN114560927A (en) * | 2022-03-15 | 2022-05-31 | 厦门元之道生物科技有限公司 | Method for producing collagen peptide by subcritical water extraction assisted fish scales and application of method |
| CN114560927B (en) * | 2022-03-15 | 2024-06-11 | 厦门元之道生物科技有限公司 | Method for producing collagen peptide by sub-critical water extraction assisted fish scales and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102702346B (en) | Method for extracting acid-soluble collagen from fish skin and extracted product | |
| CN103243144B (en) | Collagen powder rich in collagen tripeptide and preparation method thereof | |
| CN102669401A (en) | Production method of fish protein hydrolyzed chelate | |
| CN101697828A (en) | Sweet potato juice beverage and production method thereof | |
| CN109527193A (en) | A kind of preparation method for the gadus collagen peptide that phosphorylation is modified | |
| CN110205353A (en) | A kind of saline taste enhancing peptide and its preparation method and application | |
| CN104041831B (en) | A kind of tree peony polypeptide soft capsule and preparation method thereof | |
| CN101481723A (en) | Method for preparing collagen peptide by using common carp skin | |
| AU2021105562A4 (en) | Preparation Method of Tilapia Protein Peptide with Antioxidant and Antifatigue Activities | |
| CN100496277C (en) | Mantis shrimp protein pulp and its producing process | |
| CN103749689B (en) | A kind of walnut yak milk and processing method thereof | |
| CN1108109C (en) | Process for extracting soybean protein | |
| CN109943612A (en) | Method for producing high-activity minced fillet and product antifreeze agent by enzymolysis of silver carp scales | |
| CN106632665A (en) | Method for extracting collagen from freshwater fish skin by lactic acid | |
| CN107647385A (en) | A kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets | |
| US5874538A (en) | Process for producing soybean protein | |
| JPH05125100A (en) | High-purity pepsin soluble scale collagen and its production | |
| CN103549084B (en) | A kind of houttuynia extract and utilize houttuynia extract to prepare the method for cold tea and effervescent tablet | |
| CN104206640A (en) | Process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae | |
| CN101671400A (en) | Method of preparing soluble soybean polysaccharide | |
| CN101991156B (en) | Method for processing clarified antioxidative nitraria tangutorum bobr juice | |
| RU2320195C1 (en) | Method for producing of protein preparation from cyanobacteria | |
| CN108101980B (en) | Preparation method of high-purity phycocyanin | |
| CN109369775A (en) | Low purine marine fishes oligopeptide and preparation method thereof | |
| CN102422891B (en) | Fish protein formula milk powder for promoting growth and development and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |